Prevalence of the optrA gene among Streptococcus suis isolates from diseased pigs and identification of a novel integrative conjugative element ICESsu988S

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2023-09-01 DOI:10.1016/j.resmic.2023.104078

Junkai Zhang , Yingying Yang , Huarun Sun , Xingwei Luo , Xiaodie Cui , Qingqing Miao , Dandan He , Jinfeng Zhao , Fengbin Yan , Yushan Pan , Yajun Zhai , Gongzheng Hu

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Abstract

Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene optrA in Streptococcus suis (S. suis) isolates from diseased pigs in China. A total of 178 S. suis isolates were screened for the optrA gene by PCR. The phenotypes and genotypes of optrA-positive isolates were investigated by antimicrobial susceptibility testing, core genome Multilocus Sequence Typing (cgMLST), capsular serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) S. suis isolates were positive for optrA. Phylogenetic analysis indicated that the spread of the optrA among S. suis isolates was primarily due to horizontal transfer. Analysis of S. suis serotypes from diseased pigs revealed substantial diversity. The genetic environment of optrA was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICESsu988S, carrying optrA and erm(T) genes. This is to the best of our knowledge the first report of the optrA and erm(T) co-located on an ICE in S. suis. Our results showed a high prevalence of optrA gene in S. suis isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.

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optrA基因在猪链球菌病猪分离株中的患病率和一种新型整合偶联元件ICESsu988S的鉴定。

本研究的目的是调查中国猪链球菌（S.suis）病猪分离株中恶唑烷酮抗性基因optrA的流行情况和遗传环境。共178 S。用聚合酶链式反应（PCR）对猪只分离株进行optrA基因的筛选。采用抗菌药敏试验、核心基因组多基因座序列分型（cgMLST）、荚膜血清型测定和全基因组测序（WGS）对optrA阳性分离株的表型和基因型进行了研究。51株（28.7%）猪链球菌分离株optrA阳性。系统发育分析表明，optrA在猪链球菌分离株中的传播主要是由于水平转移。对患病猪的猪链球菌血清型的分析显示出显著的多样性。optrA的遗传环境复杂多样，可分为12个不同类型。有趣的是，我们发现了一种新的整合和偶联元件ICESsu988S，携带optrA和erm（T）基因。据我们所知，这是关于optrA和erm（T）在猪链球菌ICE上共定位的第一份报告。我们的结果表明，optrA基因在中国猪链球菌分离株中的高患病率。需要进一步的研究来评估ICEs的重要性，因为它们水平传播重要的临床抗性基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.