Termitidicoccus mucosus gen. nov. sp. nov. a novel Verrucomicrobiota species isolated from Reticulitermes chinensis gives insights of high adaptability of symbiotic bacteria to termite gut ecosystem

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2024-05-01 DOI:10.1016/j.resmic.2023.104173

Cheng Mei , Yu Shi , Yu Wang , Zhengyong Qiu , Hong Yang

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引用次数: 0

Abstract

Verrucomicrobiota is widely distributed in various habitats including insect guts. It was found to be prevalent in almost all investigated termite guts, whereas their physiological functions are not very clear. In this study we characterized the physiological and genomic properties of Verrucomicrobiota strain TSB47^T isolated from Reticulitermes chinensis. The cells of strain TSB47^T were Gram-stain-negative, non-motile, and non-spore-forming coccoid with one or more warts. 16S rRNA gene analysis showed that the closest relatives of strain TSB47^T were Opitutaceae strain TAV1 and Ereboglobus luteus Ho45^T (98.3% and 95.4% sequence similarity, respectively). Whole genome analysis revealed that there are a large number of glycoside hydrolase genes, amino acid metabolism genes, complete Mo-Fe nitrogenase and Fe-Fe nitrogenase gene clusters, as well as cbb₃-type cytochrome oxidase gene in the genome of strain TSB47^T. Strain TSB47^T grows well under anaerobic and microaerophilic conditions with a strong tolerance to oxygen. Physiological and genomic characters of strain TSB47^T indicated its high adaptability to termite gut ecosystem. Based on phenotypic and phylogenetic evidence, we suggest strain TSB47^T as the type species of a novel genus in the family Opitutaceae, for which the name Termitidicoccus mucosus sp. nov. is proposed. The type strain is TSB47^T (CCTCC AB2022447^T; KCTC 102044^T).

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从网纹蚁中分离出的新的白蚁微生物群（Termitidicoccus mucosus gen.

Verrucomicrobiota 广泛分布于包括昆虫内脏在内的各种生境中。研究发现，几乎所有调查过的白蚁内脏中都普遍存在这种菌群，但它们的生理功能并不十分明确。在这项研究中，我们研究了从网纹白蚁中分离出的 Verrucomicrobiota 菌株 TSB47T 的生理和基因组特性。TSB47T 菌株的细胞为革兰氏染色阴性、无运动性、无孢子形成的茧状细胞，有一个或多个疣。16S rRNA 基因分析表明，TSB47T 菌株的近亲是 Opitutaceae 菌株 TAV1 和 Ereboglobus luteus Ho45T（序列相似度分别为 98.3% 和 95.4%）。全基因组分析表明，TSB47T 菌株基因组中有大量糖苷水解酶基因、氨基酸代谢基因、完整的 Mo-Fe 氮酶和 Fe-Fe 氮酶基因簇，以及 cbb3 型细胞色素氧化酶基因。TSB47T 菌株在厌氧和微嗜氧条件下生长良好，对氧气有很强的耐受性。TSB47T 菌株的生理和基因组特征表明，它对白蚁肠道生态系统有很强的适应性。根据表型和系统发育证据，我们认为 TSB47T 菌株是 Opitutaceae 科中一个新属的模式种，并将其命名为 Termitidicoccus mucosus sp.模式菌株为 TSB47T（CCTCC AB2022447T; KCTC 102044T）。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.