Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai
{"title":"受 MtrAB 和 MprAB 调控的粘蛋白内肽酶 MepA 参与细胞壁的平衡。","authors":"Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai","doi":"10.1016/j.resmic.2024.104188","DOIUrl":null,"url":null,"abstract":"<div><p>The complete genome of <span><em>Corynebacterium glutamicum</em></span><span> contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis<span> by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that </span></span><em>mepA</em> overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-EGFP was shown to localizes exclusively at the cell cell septum. In addition, <em>mepA</em><span> overexpression increased cell permeability and reduced the resistance of cells to isoniazid, an antibiotic used to treat </span><span><em>Mycobacterium tuberculosis</em></span> infection. Furthermore, transcription analysis showed that <em>mepA</em><span> affected cell division and membrane transport pathways, and was coordinately regulated by the two-component systems MtrAB and MprAB(CgtS/R2).</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104188"},"PeriodicalIF":2.5000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis\",\"authors\":\"Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai\",\"doi\":\"10.1016/j.resmic.2024.104188\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The complete genome of <span><em>Corynebacterium glutamicum</em></span><span> contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis<span> by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that </span></span><em>mepA</em> overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-EGFP was shown to localizes exclusively at the cell cell septum. In addition, <em>mepA</em><span> overexpression increased cell permeability and reduced the resistance of cells to isoniazid, an antibiotic used to treat </span><span><em>Mycobacterium tuberculosis</em></span> infection. Furthermore, transcription analysis showed that <em>mepA</em><span> affected cell division and membrane transport pathways, and was coordinately regulated by the two-component systems MtrAB and MprAB(CgtS/R2).</span></p></div>\",\"PeriodicalId\":21098,\"journal\":{\"name\":\"Research in microbiology\",\"volume\":\"175 5\",\"pages\":\"Article 104188\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0923250824000123\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0923250824000123","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis
The complete genome of Corynebacterium glutamicum contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that mepA overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-EGFP was shown to localizes exclusively at the cell cell septum. In addition, mepA overexpression increased cell permeability and reduced the resistance of cells to isoniazid, an antibiotic used to treat Mycobacterium tuberculosis infection. Furthermore, transcription analysis showed that mepA affected cell division and membrane transport pathways, and was coordinately regulated by the two-component systems MtrAB and MprAB(CgtS/R2).
期刊介绍：
Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.
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