Research in veterinary science最新文献

{"title":"Beneficial effects of probiotics on dysbiosis of gut microbiota induced by antibiotic treatment in healthy dogs","authors":"Sung-Jae Kim ,&nbsp;Hee-Chun Chung ,&nbsp;Soo-Yeon Park ,&nbsp;Jae-Myun Lee ,&nbsp;Jeong-Hee Han","doi":"10.1016/j.rvsc.2025.105674","DOIUrl":"10.1016/j.rvsc.2025.105674","url":null,"abstract":"<div><div>The gut microbiota plays a crucial role in maintaining host health. While numerous studies have explored the impact of antibiotics on the gut microbiota in humans, limited research has examined how antibiotics affect the gut microbiome in dogs. This study investigated the effects of antibiotic treatment on the gut microbiota of dogs and assessed whether probiotic supplementation could prevent antibiotic-induced dysbiosis. Fourteen healthy young dogs undergoing castration were included in the study. All dogs received a single injection of cefovecin immediately after surgery. The probiotics group (7 dogs) was given a probiotic complex daily starting on the day of surgery and continuing for two weeks, while the non-probiotics group (7 dogs) received no supplementation. Fecal samples were collected on the day of surgery and two weeks later during the follow-up visit for suture removal for microbiome analysis. In microbial diversity analysis, α-diversity was significantly higher in the probiotic-supplemented group compared to the non-probiotics group (<em>p</em> &lt; 0.05). β-diversity analysis revealed significant differences in microbial community composition in the non-probiotics group after antibiotic treatment (<em>p</em> &lt; 0.05), while no significant differences were observed in the probiotics group. Relative abundance analysis indicated that <em>Clostridioides</em>, a marker of antibiotic-induced dysbiosis, significantly increased in dogs without probiotics after antibiotic treatment (<em>p</em> &lt; 0.05). In contrast, <em>Butyricicoccus</em>, a butyrate-producing bacterium with gut health benefits, was significantly enriched in the probiotics group (<em>p</em> &lt; 0.05). These findings suggest that probiotic supplementation supports healthier gut microbiome recovery following antibiotic treatment and highlights its potential to enhance gut microbiota restoration and mitigate gut dysbiosis caused by antibiotics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105674"},"PeriodicalIF":2.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac biomarkers N-terminal fragment of the prohormone B-type natriuretic peptide and cardiac troponin I for characterization of the cardiac disease phenotype of the English Bull Terrier","authors":"C. Chompoosan ,&nbsp;J. Koch,&nbsp;A.S. Schrøder,&nbsp;M.B.T. Bach,&nbsp;J.L. Willesen,&nbsp;R. Langhorn","doi":"10.1016/j.rvsc.2025.105686","DOIUrl":"10.1016/j.rvsc.2025.105686","url":null,"abstract":"<div><div>The N-terminal fragment of prohormone B-type natriuretic peptide (NT-proBNP) and cardiac troponin I (cTnI) contribute information regarding cardiac load and function and myocardial injury, respectively, to the clinical work-up of dogs with heart disease. The English bull terrier (BT) has a predisposition to a complex cardiac phenotype. This prospective case-control study aimed to further characterize this phenotype through cardiac biomarker evaluation.</div><div>Fifteen healthy BT (group 1) and 50 BT with various heart disease presentations (groups 2–4) were included. The main presentations were MVD (45 dogs) and MS (13 dogs). 16/50 dogs had echocardiographic evidence of cardiac remodeling: 7 with either increased left atrial (LA) or left ventricular (LV) volumes (group 3) and 9 with increased LA and LV volumes (group 4).</div><div>Cardiac biomarkers were not significantly different between dogs with disease subtypes MVD or MS versus dogs with other disease presentations. When evaluated in relation to remodeling, median NT-proBNP and cTnI in group 4 (1004 pmol/L, 0.09 ng/mL) were significantly higher than in group 1 (257 pmol/L, 0.02 ng/mL), group 2 (300 pmol/L, 0.026 ng/mL) and group 3 (250 pmol/L, 0.02 ng/mL). There was significant discriminatory ability for both biomarkers between group 4 and groups 1, 2 or 3. Furthermore, cTnI significantly differentiated dogs with any degree of remodeling (groups 3 + 4) from those without (groups 1 + 2).</div><div>The cardiac disease phenotype of the BT is not associated with a unique cardiac biomarker profile. Rather, biomarker levels are similar to those reported in other breeds with valvular heart disease.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105686"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereological and morphometric insights into epididymal development in domestic cats (Felis silvestris catus) from 6 to 48 months","authors":"P. Salinas,&nbsp;D. Escobar","doi":"10.1016/j.rvsc.2025.105690","DOIUrl":"10.1016/j.rvsc.2025.105690","url":null,"abstract":"<div><div>This study characterizes age-related morphometric and morphological changes in the epididymis of domestic cats (<em>Felis silvestris catus</em>) from puberty to adulthood (6 to 48 months), emphasizing its essential role in sperm maturation and storage—key processes for male fertility. A total of 42 epididymides were analyzed using histological staining (hematoxylin-eosin) and stereological quantification through the STEPanizer software. Morphometric analyses revealed an age-dependent increase in the diameter of the epididymal duct and epithelial height in the <em>caput</em>, whereas the <em>cauda</em> exhibited a progressive reduction in epithelial height, possibly reflecting adaptations in sperm storage capacity during sexual maturation. Morphological observations showed the presence of intraepithelial cysts in cats aged 24 to 48 months, along with the consistent detection of spermatozoa in all regions and age groups. Stereological findings indicated an increased volumetric density (%VV) of the ductal epithelium, particularly in the <em>caput</em> between 6 and 12 months of age, supporting the influence of androgenic activity on regional epididymal maturation. These changes suggest dynamic, age-related structural remodeling of the epididymal parenchyma, especially in epithelial and luminal components. While this cross-sectional study—conducted during the southern hemisphere spring—provides valuable insights into epididymal development, its design limits the establishment of causal relationships between age and histological changes. Future longitudinal studies examining hormonal modulation of epididymal maturation in domestic cats are encouraged. Overall, these findings contribute foundational knowledge of feline reproductive anatomy and underscore the importance of the epididymis as a hormonally responsive organ central to male fertility.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105690"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in the tear film lipid layer by age in healthy brachycephalic dogs","authors":"Alessandro Cirla ,&nbsp;Valentina Andreani ,&nbsp;Angelo Pasquale Giannuzzi ,&nbsp;Laura Ventura ,&nbsp;Giovanni Barsotti","doi":"10.1016/j.rvsc.2025.105684","DOIUrl":"10.1016/j.rvsc.2025.105684","url":null,"abstract":"<div><div>This cross-sectional study evaluates the morphological and qualitative changes in the meibomian glands (MGs) and tear film lipid layer (TFLL) associated with aging and sex in canine brachycephalic breeds. One hundred and eleven brachycephalic dogs with normal eyes were included. Dogs were divided into three age groups: ≤4 years (G1), &gt;4 and &lt; 10 years (G2) and ≥ 10 years (G3). Schirmer test I (STT I), upper eyelid MGs noncontact infrared meibography (NIM), tear interferometry (TFI) and tear film breakup time (TFBUT) were performed in each eye. Loss of MGs and lid margin abnormalities (LMAs), as well as TFI patterns were scored from 0 to 3 depending on severity. Normality was assessed with the Shapiro-Wilk test. The Kruskal-Wallis test was used for the analysis of variance among groups. Pearson's or Spearman's correlation coefficient was used to assess the correlation between MG loss and STT I values. Significance was set at <em>p</em> &lt; 0.05. Forty-four Shih-Tzus, 37 Pugs and 30 French Bulldogs were enrolled in the study. Median age was 39.5 months (range 30–48 months) in G1, 94.5 months (range 65–118 months) in G2, 154.5 months (range 120–201 months) in G3. Loss of MGs was statistically higher in G2 and G3 (<em>p</em> &lt; 0.001) and was associated with a reduction in TFI scores (p &lt; 0.001) and STT I (p &lt; 0.001). Loss of MGs was then negatively correlated with TFBUT. No statistical differences were associated with sex (<em>p</em> &gt; 0.05) or breed (p &gt; 0.05). The tear film lipid layer appears to be negatively affected by age in brachycephalic dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105684"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from African swine fever virus-infected pigs mediate immune responses through NF-κB and JAK-STAT signaling pathways","authors":"Suyeon Kang ,&nbsp;Thi Hao Vu ,&nbsp;Chaeeun Kim ,&nbsp;Anh Duc Truong ,&nbsp;Yeong Ho Hong","doi":"10.1016/j.rvsc.2025.105687","DOIUrl":"10.1016/j.rvsc.2025.105687","url":null,"abstract":"<div><div>African swine fever (ASF) virus (ASFV) is an infectious disease that affects the pig industry, causing up to 85 % morbidity and 100 % mortality. To date, there are no available vaccines against ASFV. Exosomes are extracellular vesicles that are released from most cell types. Exosomes carry components such as nucleic acids, lipids, and proteins that play a vital role in cell-to-cell communication. This study investigated the effect of exosomes derived from the serum of ASFV-infected pigs on a porcine macrophage cell line. Exosomes derived from the serum of pigs infected with ASFV contained ASFV structural proteins (p30 and p72). Expression levels of interferon (IFN)-α, IFN-γ, IL-6, and CXCL8 in porcine macrophage cells were affected by exposure to exosomes derived from the serum of ASFV-infected pigs. Nuclear factor-κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway play an important role in the immune response to ASFV infection. Exosomes derived from ASFV-infected pigs affected mRNA and protein levels of NF-κB, tank binding kinase 1 (TBK1), JAK1, JAK2, and STAT1, suggesting that exosomes derived from ASFV-infected pigs mediate antiviral response by modulating the expression of inflammatory cytokines and activity of the NF-κB and JAK-STAT signaling pathways. The present study provides novel information about the immunomodulatory effects of exosome derived from pigs infected with ASFV, improving our understanding of ASFV pathogenesis and the host immune response to ASFV infection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105687"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics and metabolomics reveal the role of miR-320b in regulating inflammation of bovine mammary epithelial cells","authors":"Li Zhou ,&nbsp;Zhuo-Ma Luoreng ,&nbsp;Xing-Ping Wang ,&nbsp;Ya-Qing Dou ,&nbsp;Hui Li","doi":"10.1016/j.rvsc.2025.105682","DOIUrl":"10.1016/j.rvsc.2025.105682","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that are widely found in organisms and play an important regulatory role in various biological processes, especially immune and inflammatory responses. However, the function of miR-320b in the inflammatory responses of bovine mammary epithelial cells (bMECs) remains to be elucidated. In this study, we examined the miR-320b mimic transduction group (miR-320b_mimic) and negative control mimic transduction group (NC_mimic) of lipopolysaccharide-treated bMECs using data-independent acquisition (DIA) proteomics and untargeted metabolomics. Subsequently, we performed a joint analysis of the sequencing data. Proteomic analysis identified 330 differentially abundant proteins (DAPs) primarily related to PPAR, ferroptosis, arachidonic acid metabolism, IL-17, and complement and coagulation cascades. Metabolome analysis identified 128 and 66 differentially accumulated metabolites (DAMs) in the positive and negative ion mode primarily involved in linoleic acid metabolism, cholesterol metabolism, AMPK, MAPK, and chemokine. Integrated metabolomics and proteomics analysis revealed the co-enrichment of DAPs and DAMs in choline metabolism in cancer, endocrine resistance, glycerophospholipid metabolism, primary bile acid biosynthesis, and the ferroptosis signaling pathways. The results of quantitative real-time PCR (RT-qPCR) showed that compared with the NC_mimic group, mRNA expression levels of <em>COX-2, IL-12 A, iNOS, MAPK1,</em> and <em>MAPK14</em> genes were significantly down-regulated, and the mRNA expression levels of <em>PPARγ, CEBPα, CEBPβ, FABP4,</em> and <em>LPL</em> genes were significantly up-regulated in the miR-320b_mimic group. These results provide crucial insights into the molecular regulatory functions of miR-320b and offer valuable data for further research on molecular breeding aimed at enhancing mastitis resistance in bovine animals.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105682"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection, epidemiology and phylogenetic evaluation of Babesia ovis in apparently healthy goats","authors":"Asia Taqddus ,&nbsp;Muhammad Naeem ,&nbsp;Hira Muqaddas ,&nbsp;Ceylan Ceylan ,&nbsp;Onur Ceylan ,&nbsp;Ferda Sevinc ,&nbsp;Maryam Rahravani ,&nbsp;Meysam Moravedji ,&nbsp;Alireza Sazmand ,&nbsp;Furhan Iqbal","doi":"10.1016/j.rvsc.2025.105672","DOIUrl":"10.1016/j.rvsc.2025.105672","url":null,"abstract":"<div><div><em>Babesia</em> (<em>B</em>.) <em>ovis</em> is an intra-erythrocytic protozoan parasite that infects small ruminants globally, causing economic losses. This study aimed to investigate the molecular prevalence of <em>B. ovis</em> in 1200 asymptomatic goats of various breeds across four districts in Punjab, Pakistan: Layyah, Lohdran, Dera Ghazi Khan, and Rajanpur. The enrolled goats represented ten breeds, including Daira Din Pannah (<em>n</em> = 890), Pahari goat (<em>n</em> = 68), Nukri (<em>n</em> = 44), Teddy (<em>n</em> = 37), Lail Puri (<em>n</em> = 36), Beetal (<em>n</em> = 36), Dessi (<em>n</em> = 32), Makhi Cheena (<em>n</em> = 27), Muhammad Puri (<em>n</em> = 19) and Fazil Puri (<em>n</em> = 11). The hematological and biochemical profiles of the goats, risk factors associated with the infection, and the phylogenetic relationship of the detected isolates were also evaluated. In total, 105 blood samples (9.6 %) tested positive by PCR. Sanger sequencing of a partial fragment of the <em>18S rRNA</em> gene confirmed <em>B. ovis</em>. Phylogenetic analysis of the <em>18S rRNA</em> gene sequences revealed 99–100 % similarity with isolates previously reported from Iran, Iraq, Turkey, and Spain. The infection rate varied across districts, with the highest prevalence observed in goats from Rajanpur (13 %), followed by Dera Ghazi Khan (11 %), Layyah (7 %), and Lohdran (5 %) (<em>P</em> = 0.003). The susceptibility to infection varied among goat breeds, with Lail Puri breed showing the highest susceptibility (<em>P</em> = 0.03). Risk factor analysis revealed that goats under one year of age and those kept on farms where other animals and dogs were also present had higher <em>B. ovis</em> infection rates. <em>Babesia ovis</em>-infected goats showed reductions in white and red blood cells, hemoglobin concentration, and alterations in serum aspartate aminotransferase and creatinine levels. This study provides updated data on the prevalence of <em>B. ovis</em> in local Pakistani goat populations, emphasizing the need for integrated control strategies against this tick-borne pathogen.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105672"},"PeriodicalIF":2.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer registration in dogs and cats: A narrative review of history, current status, and standardization efforts","authors":"Niccolò Fonti ,&nbsp;Francesca Millanta","doi":"10.1016/j.rvsc.2025.105673","DOIUrl":"10.1016/j.rvsc.2025.105673","url":null,"abstract":"<div><div>Cancer severely affects the health of companion animals, with neoplasia being a leading cause of death in pets. Although pets and humans share the home environment and dogs and cats can serve as sentinel species for environmental carcinogens, cancer surveillance in pets remains fragmented after decades of studies. The aim of this narrative review is to assess the evolution and current status of animal cancer registries (ACRs), highlighting historical milestones and key methodologies with a global perspective. The different types of cancer registries, their different roles and related issues in cancer surveillance are highlighted. Inconsistent diagnostic criteria, limited standardization, and lack of centralized databases hinder the comparability of results in veterinary oncology. Recent initiatives, such as the Global Initiative for Veterinary Cancer Surveillance (GIVCS) and the Veterinary Cancer Guidelines and Protocols (VCGP), seek to standardize cancer reporting and coding systems and promote a One Health approach that links veterinary and human oncology research. Increased standardization and data sharing between databases is critical to advancing cancer surveillance in companion animals, benefiting both veterinary and public health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105673"},"PeriodicalIF":2.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Characterization and validation of bovine Gonadotropin Releasing Hormone Receptor (GnRHR) polymorphisms” [Research in Veterinary Science 91/3 (2010) 1774]","authors":"J.P. Lirón ,&nbsp;A. Prando ,&nbsp;M.V. Ripoli ,&nbsp;A. Rogberg-Muñoz ,&nbsp;D.M. Posik ,&nbsp;A. Baldo ,&nbsp;P. Peral-García ,&nbsp;G. Giovambattista","doi":"10.1016/j.rvsc.2025.105632","DOIUrl":"10.1016/j.rvsc.2025.105632","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105632"},"PeriodicalIF":2.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot study on the use of an off-the-shelf 3D-printed titanium acetabular rim extension implant for treating canine hip dysplasia","authors":"Irin Kwananocha ,&nbsp;Joëll Magré ,&nbsp;Femke Verseijden ,&nbsp;Amir S. Kamali ,&nbsp;Saber Amin Yavari ,&nbsp;Saskia G.M. Plomp ,&nbsp;Ralph J.B. Sakkers ,&nbsp;Marianna A. Tryfonidou ,&nbsp;Harrie Weinans ,&nbsp;Koen Willemsen ,&nbsp;Bart C.H. van der Wal ,&nbsp;Björn P. Meij","doi":"10.1016/j.rvsc.2025.105668","DOIUrl":"10.1016/j.rvsc.2025.105668","url":null,"abstract":"<div><div>Hip dysplasia (HD) is one of the most prevalent orthopedic issues in dogs. Generic shelf implants are 3D-printed devices designed to address HD in dogs of varying sizes and hip laxity scores. This pilot study evaluated the feasibility and effectiveness of generic shelf implants in six dogs diagnosed with bilateral HD. Each dog underwent bilateral hip surgery in a single session followed by a 12-month monitoring period.</div><div>Post-operative CT scans revealed increased femoral head coverage in all 12 hips, resulting in reversed hip laxity. However, implants exhibited positional deviations from optimal congruency for 5.1 ± 1.7 mm. Mild to moderate osteoarthritis (OA) progression was observed during the lead times and post-operatively. Although force plate analysis showed no significant changes, functional outcomes improved, as demonstrated by reduced owner questionnaire scores at 12 months post-surgery. Post-operative internal rotation and abduction angles were reduced compared to pre-operative values, suggesting potential impingement due to implant positioning. Complications were observed in one dog (two hips). Another dog died nine months post-surgery due to unrelated causes. Post-mortem histology and micro-CT analysis of these hip joints revealed localized cartilage depletion around the implant's rim extension area. However, no lameness or hip discomfort was reported by the owner before the dog's death.</div><div>The generic shelf implant effectively enhances hip stability and alleviates pain-related behaviors by improving femoral head coverage. Suboptimal placement was associated with reduced range of motion and OA progression in this study. Refinements to the implant design and adjusting sizing protocols to better match acetabular morphology are necessary.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105668"},"PeriodicalIF":2.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}