Research in veterinary science最新文献

{"title":"Antimicrobial and antibiofilm in vitro effect of oxygen-ozone gas against Staphylococcus aureus","authors":"Fernanda Calmon Blanc ,&nbsp;Isabela Sant Anna Dombrowski ,&nbsp;Naiane Ferreira de Oliveira ,&nbsp;Sandryelle Mercês Freire da Silva ,&nbsp;Júlia Calcado Moreira ,&nbsp;Carolina Oliveira Santos ,&nbsp;Miguel Gabriel Ribeiro Miguel ,&nbsp;Matheus Gomes Salvado ,&nbsp;Bruno de Araújo Penna ,&nbsp;Aline Emerim Pinna","doi":"10.1016/j.rvsc.2025.105814","DOIUrl":"10.1016/j.rvsc.2025.105814","url":null,"abstract":"<div><div>Equine endometritis is the primary reproductive pathology that causes significant economic losses due to subfertility, and <em>Staphylococcus aureus</em> (<em>S. aureus</em>) is one of the main infectious agents. Oxygen-ozone is a gas with high oxidative potential that promotes the destruction of bacterial cells, modulates inflammation, and induces a regenerative response, making it an adequate alternative treatment for endometritis. This study aimed to evaluate the antimicrobial and antibiofilm effects of the oxygen-ozone gas on microbial cultures of <em>S. aureus</em>. Closed ozonated systems were used in three experiments. The <em>S. aureus</em> cultures were then treated with the oxygen-ozone mixture by either bubbling the gas through the Tryptic Soy Broth with the bacteria or directly exposing the plates to the gas while testing different amounts and lengths of the exposure. In the first experiment, it was observed that the bactericidal effect on the planktonic state increased after 10 min, and showed a better result at 60 min. In experiment two a bactericidal effect with exposure for 10 min to concentrations of 19 and 41 μg/mL was observed. After exposure for 20 min, a concentration of 10 μg/mL was effective in killing the strain in the planktonic state. Antibiofilm effects were observed at concentrations of 19, 41, and 70 μg/mL. Therefore, it can be concluded that <em>S. aureus</em> showed sensitivity to oxygen-ozone gas, which also promoted an antibiofilm effect on the studied strain.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105814"},"PeriodicalIF":1.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin improves growth and slaughter performance of broilers by regulating satellite cell proliferation via RORα nuclear receptor","authors":"Shuhui Ma ,&nbsp;Lina Dong ,&nbsp;Aili Fan ,&nbsp;Yang Li ,&nbsp;Yuzhe Fan ,&nbsp;Jiayin Liu ,&nbsp;Liang Hong ,&nbsp;Zonghui Zuo ,&nbsp;Daowen Li ,&nbsp;Gang Xu","doi":"10.1016/j.rvsc.2025.105823","DOIUrl":"10.1016/j.rvsc.2025.105823","url":null,"abstract":"<div><div>To investigate the molecular mechanisms of melatonin on the growth and slaughter performance of broilers, 144 newly hatched male broilers were randomly assigned to dietary melatonin (0, 5, 20, or 80 mg/kg) for 42 days. Chicken skeletal muscle satellite cells (SMSCs) were treated with melatonin (0.5–312.5 ng/mL), and retinoid-related orphan receptor-alpha (RORα) involvement was investigated using agonist SR1078 or inverse agonist SR3335. Growth and slaughter performance, serum antioxidant capacity, meat quality, as well as molecular expression levels analyzed for skeletal muscle growth related factor were conducted. The results demonstrated dietary melatonin significantly decreased feed gain ratio, and elevated muscle percentage (<em>P &lt;</em> 0.05), serum melatonin content (<em>P &lt;</em> 0.05), total antioxidation capability (T-AOC) and the activity of antioxidant enzymes (<em>P &lt;</em> 0.05), while reduced the levels of malondialdehyde (MDA) (<em>P &lt;</em> 0.05). Administration of melatonin notably improved the water-holding capacity and myofiber area of muscles (<em>P &lt;</em> 0.05). Additionally, melatonin significantly upregulated the expression of paired domain homeobox transcription factor 7 (Pax7), myoblast determination protein (MyoD), and myogenic factor 5 (Myf5) in muscles and SMSCs, downregulated Myostatin (MSTN) (<em>P &lt;</em> 0.05). In the presence of melatonin, the SR1078 markedly enhanced Pax7 expression, while the SR3335 reduced the expression of Pax7, MyoD, and Myf5 (<em>P &lt;</em> 0.05). In conclusion, melatonin has the potential to regulate the proliferation of chicken SMSCs through the RORα nuclear receptor, affecting the expression levels of skeletal muscle growth related factor in SMSCs, promoting the growth and slaughter performance of broilers.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105823"},"PeriodicalIF":1.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profile as a prime marker of fertility detection in Egyptian dairy buffaloes (Bubalus bubalis)","authors":"Seham Samir Soliman PhD ,&nbsp;Sherif M. Afifi ,&nbsp;Banny Silva Barbosa Correia ,&nbsp;Abdelaziz M. Sakr ,&nbsp;Mohammed Mahmoud Hegazy ,&nbsp;Naglaa M. Ammar","doi":"10.1016/j.rvsc.2025.105822","DOIUrl":"10.1016/j.rvsc.2025.105822","url":null,"abstract":"<div><div>Buffalo reproductive performance is frequently compromised by postpartum anestrus and repeat breeding, yet the underlying metabolic mechanisms remain largely uncharacterized. This study aimed to identify serum metabolomic differences among four reproductive status groups, smooth inactive ovaries (SIO), repeat breeders (RB), normal cyclic, and pregnant buffalo under thermal-neutral conditions. Twenty-eight adult healthy buffalo cows (4–6 years old, 400–500 kg) were classified by reproductive status using transrectal ultrasonography and rectal palpation. Serum metabolomic analysis revealed distinct profiles among the groups. Infertile buffalo (SIO and RB) exhibited significant disruptions in amino acid, fatty acid, and bile acid metabolism compared to fertile animals. Indole, acetamide, and carbodiimide were relevant to distinguishing the repeat breeder group, while methylmalonic acid and heptadecanoic acid had their changing relevance in the smooth inactive ovaries’ groups. Methylamine, phenylalanine, and galactose methyloxyme discriminate the control group. In contrast, propanediol, urea and carbamic acid were abundant in the pregnant group. These findings suggest that metabolic imbalances in specific pathways are associated with reproductive dysfunctions and may serve as biomarkers for fertility assessment. This integrative approach combining physiological classification and metabolomic profiling enhances understanding of fertility-related metabolic alterations and offers potential for improving reproductive management in buffalo.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105822"},"PeriodicalIF":2.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anticoccidial efficacy of Houttuynia cordata: Insights into gut microbiota modulation and metabolic enhancement in lambs","authors":"Shuaiqi Liu ,&nbsp;Shiheng Li ,&nbsp;Shucheng Huang ,&nbsp;Manyu Liu ,&nbsp;Sumei Zhang ,&nbsp;Longxian Zhang ,&nbsp;Senyang Li ,&nbsp;Fuchun Jian","doi":"10.1016/j.rvsc.2025.105821","DOIUrl":"10.1016/j.rvsc.2025.105821","url":null,"abstract":"<div><div><em>Eimeria</em> primarily infects the intestinal epithelial cells of livestock and poultry, disrupting gut microbiota and metabolism, which can result in diarrhea and weight loss. In severe cases, it can lead to hematochezia and even mortality. Modern intensive and large-scale farming practices, combined with the fecal-oral transmission of coccidia, create environments conducive to coccidial infections. Chemical control methods may lead to drug residues, drug resistance, toxic side effects, and other related issues. In contrast, Chinese herbal medicines typically exhibit multi-target, multi-pathway, and synergistic properties in disease resistance. In this study, the McMaster counting method was employed to assess the anti-coccidial effects of <em>Houttuynia cordata</em> (<em>H. cordata</em>) in its untreated forms (raw, water extract, and alcohol extract). The impact of these treatments on the intestinal microbiota and metabolites of lambs was examined using 16S rRNA gene sequencing technology and non-targeted metabolomics. The findings indicated that <em>H. cordata</em> reduced the excretion of intestinal coccidian oocysts in lambs. Notably, the alcohol extract of <em>H. cordata</em> (HA) significantly enhanced the average daily weight gain (ADG) and increased the abundance of unclassified <em>Lachnospiraceae</em>, <em>Christensenellaceae R_7</em> group, and <em>UCG_005</em>. Additionally, metabolites such as 6-Deoxyerythronolide B, Parthenolide, and Bacilysin were highlighted for their potential significance. Overall, HA demonstrated superior anticoccidial effects and improved weight gain, likely due to the enhancement of beneficial microbiota and metabolites. This study provides a theoretical foundation for understanding the mechanisms by which Chinese herbal medicine combats coccidiosis through the modulation of intestinal microbiota and metabolism.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105821"},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative anatomy analysis of the accessory carpal bone across dog and cat breeds","authors":"Yusuf Altundağ ,&nbsp;Nicoleta Manuta ,&nbsp;Ermiş Özkan ,&nbsp;Sokol Duro","doi":"10.1016/j.rvsc.2025.105817","DOIUrl":"10.1016/j.rvsc.2025.105817","url":null,"abstract":"<div><div>This study investigates the morphological variation of the accessory carpal bone in mixed-breed cats and dogs of different breeds, focusing on 3D shape differences across species. Geometric morphometric analysis was applied to 3D models of 10 dogs and 15 cats, aiming to explore species-specific adaptations in carpal bone morphology. Cats (mix breeds) exhibited negative values for PC1, indicating more compact and slender carpal bones, consistent with their enhanced mobility and smaller body sizes. Both small and large dog breeds displayed positive PC1 values, indicating that the observed shape variation does not directly reflect body size but rather species- or breed-specific morphology. These findings were supported by centroid size analysis, which correlated with the shape variation along PC1. The lack of significant correlations between accessory carpal bone shape and Procrustes distance suggests that, while this may influence skeletal morphology, genetic factors likely play a more substantial role in shaping the carpal bone. This study highlights the utility of geometric morphometry in detecting species-specific skeletal adaptations and emphasizes the potential of this method to explore functional morphology in domestic carnivores. In conclusion, understanding the morphological variation of the accessory carpal bone in dogs and cats mixed breeds contributes to veterinary anatomy and has practical applications in veterinary medicine, particularly in surgical and rehabilitation practices. Therefore, the results contribute to a deeper understanding of carpal bone variation in cats and dogs, with implications for functional and evolutionary studies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105817"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational success in a female dog following early repair of an inguinal gravid hysterocele","authors":"Gisele Almeida Lima da Veiga ,&nbsp;Mariana Semião Francisco Talib ,&nbsp;Marcelo Faustino ,&nbsp;Roberto Rodrigues da Rosa Filho ,&nbsp;Monike Alves Lopes ,&nbsp;Guilherme Santos Souza ,&nbsp;Laura Lima do Val Carneiro Machado ,&nbsp;Camila Infantosi Vannucchi","doi":"10.1016/j.rvsc.2025.105818","DOIUrl":"10.1016/j.rvsc.2025.105818","url":null,"abstract":"<div><div>Inguinal gravid hysterocele is a pregnancy disorder characterized by the presence of the uterus as the contents of an inguinal hernia. This condition often progresses to strangulation of the uterus and uterine vessels, leading to reduced blood flow to the placenta and, consequently, fetal death. This case report details the clinical presentation, diagnostic findings, surgical approach, and perinatal outcomes of a 4-year-old, 17 kg, multiparous American Bully bitch, presented 25 days post-artificial insemination for pregnancy confirmation. Clinical examination revealed bilateral, non-reducible, painless inguinal swellings. Ultrasonographic assessment identified two gestational vesicles within the right inguinal canal and a third in the abdominal cavity, consistent with a diagnosis of bilateral inguinal hernia and unilateral gravid hysterocele. Due to the potential for uterine incarceration and compromise of maternal-fetal viability, early surgical intervention was elected. The postoperative period was uneventful, with serial imaging and laboratory monitoring confirming adequate fetal development and maternal stability. An elective cesarean section was performed at term (60 days of gestation), resulting in the delivery of two viable neonates without congenital anomalies. This case report underscores the importance of early recognition and timely surgical intervention in inguinal gravid hysterocele to preserve maternal health and fetal viability. It also addresses the diagnostic and therapeutic challenges posed by low-prevalence obstetric conditions, emphasizing the need for rigorous prenatal monitoring, rapid and accurate clinical decision-making, and effective interdisciplinary collaboration to ensure favorable outcomes. Moreover, the systematic dissemination of such clinical experiences to the scientific community is essential for the ongoing development and refinement of evidence-based protocols for the management of obstetric emergencies in veterinary medicine.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105818"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of Bovine Gammaherpesvirus-4 (BoHV-4) in diarrheic sheep","authors":"Kadir Yeşilbağ ,&nbsp;Mevlüt Yaşar ,&nbsp;Zafer Ata","doi":"10.1016/j.rvsc.2025.105819","DOIUrl":"10.1016/j.rvsc.2025.105819","url":null,"abstract":"<div><div>While commonly detected in cattle, the presence of <em>Bovine gammaherpesvirus 4</em> (BoHV-4) in small ruminants remains insufficiently examined. This study reports the isolation, identification, and genetic characterization of BoHV-4 from a sheep presenting hemorrhagic diarrhea. A fecal sample collected from an infected adult sheep was submitted to a diagnostic study using a real-time PCR panel for various bacteria, parasites, and viruses, yielding negative results for all the pathogens in the panel. Virus isolation was performed on MDBK, BHK-21, SFT-R cell lines, and primary lamb kidney cell culture (PLK), and cytopathogenic effects were observed in all the cell lines except for PLK. The virus was then identified through chloroform and halogen assays. The presence of BoHV-4 was validated by nested PCR targeting the thymidine kinase (TK) gene, and phylogenetic analysis classified the isolate as Genotype II, showing close genetic similarity with Argentinean strains. Serum neutralization assay demonstrated low cross-reactivity between BoHV-1 polyclonal serum and BoHV-4 isolate. This study reports the first detection of BoHV-4 genotype II in sheep and suggests its potential association with gastrointestinal disease. Further studies are needed to investigate the pathogenic role of BoHV-4 in small ruminants and its impact on various organ systems.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105819"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary probiotic prototypes and their effects on growth performance, immune function, and gut microbiota of rainbow trout (Oncorhynchus mykiss)","authors":"Sahar Mahmoodian ,&nbsp;Amir Meimandipour ,&nbsp;Monireh Faeed ,&nbsp;Mehdi Shamsara ,&nbsp;Javad Daghigh roohi ,&nbsp;Seyed Safa-Ali Fatemi ,&nbsp;Mohades Ghasemi ,&nbsp;Mahsa Chaharmahali","doi":"10.1016/j.rvsc.2025.105808","DOIUrl":"10.1016/j.rvsc.2025.105808","url":null,"abstract":"<div><div>This study focused on developing novel probiotic formulations for rainbow trout aquaculture by testing two formulations containing bacterial strains isolated from the gut of rainbow trout. The effects on growth, immune response, liver enzyme activity, immunity-related gene expression, and gut microbiota composition were evaluated. A completely randomized design was used, involving 450 rainbow trout distributed into 70-l fiberglass tanks with six dietary treatments and three replicates per treatment (total of 18 tanks). Treatments A and B contained three strains of <em>Pediococcus</em> sp. P15, <em>Lactococcus lactis</em> ml3, and <em>Weissella cibaria</em> ml6 administered at two dosages (300 and 500 mg/kg). Treatments C and D included five strains of <em>Pediococcus</em> sp. P15, <em>Lactococcus lactis</em> ml3, <em>Weissella cibaria</em> ml6, <em>Enterococcus faecium</em> E10, and <em>Lactobacillus curvatus</em> 13 A also at 300 and 500 mg/kg. Treatment E was a commercial probiotic at 300 mg/kg, and treatment F served as the control without probiotics. Fish treated with A, B, and E showed significantly higher final weights than the control, with A and B groups exhibiting the lowest feed conversion ratios. Groups A and D had significantly increased immunoglobulin M (<em>IgM</em>) levels. Tumor necrosis factor (<em>TNF</em>) expression decreased in group A, while interleukin 6 (<em>IL6</em>) and immunoglobulin T (<em>IgT</em>) expressions increased by 4.4 and 4.22-fold, respectively. Treatment A also resulted in higher counts of lactic acid bacteria in the gut. Overall, treatment A demonstrated the greatest potential to improve growth, immunity, and gut microbiota, making it a promising probiotic candidate for rainbow trout farming.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105808"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormone receptor discrepancy and molecular subtype changes in canine mammary carcinomas and synchronous lymph node metastasis","authors":"Tiago Ferreira ,&nbsp;Ana Teresa Machado ,&nbsp;Rui Medeiros ,&nbsp;João F. Mano ,&nbsp;Paula A. Oliveira ,&nbsp;Adelina Gama","doi":"10.1016/j.rvsc.2025.105816","DOIUrl":"10.1016/j.rvsc.2025.105816","url":null,"abstract":"<div><div>Mammary tumors are the most prevalent neoplasms in intact female dogs; up to 50 % are considered malignant and have the potential to metastasize to regional lymph node and distant organs. Changes in tumor molecular subtype, including its receptor status, can occur during mammary tumor progression. This study aimed to explore hormone receptor discrepancies and the relationship between molecular subtypes in primary mammary tumors (PTs) and paired lymph node metastases (LNMs). Thirty PTs samples and paired LNMs were evaluated for estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 through immunohistochemistry. After analysis, the discordance rates were 11.1 %, 26.9 %, and 11.5 %, for ER, PR and Ki-67, respectively. All samples were negative for HER2. The molecular subtypes showed a 40.7 % discordance rate between PTs and matched LNMs. Additionally, 44.4 % of luminal A-like, 36.4 % of luminal B-like and 42.8 % of triple-negatives in PTs displayed different subtypes in the corresponding LNMs, respectively. Among discordant cases, five progressed to subtypes usually associated with a poor prognosis, while six presented a transition to subtypes frequently associated with a favorable prognosis. Discordance in molecular subtypes between PTs and LNMs may lead to therapeutic failures. Therefore, the assessment of molecular subtypes at both sites is crucial and could offer potential for the development of more accurate prognostic and treatment models.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105816"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DIA-based serum proteomics in goat pregnancy: biomarkers & mechanisms","authors":"Dongliang Xu ,&nbsp;Di Wang ,&nbsp;Lisheng Zhou,&nbsp;Zhengang Huang,&nbsp;Mingzhi Sun,&nbsp;Xinxin Cao,&nbsp;Jianyu Zhou,&nbsp;Xiaoxiao Gao,&nbsp;Jianning He,&nbsp;Jinshan Zhao,&nbsp;Hegang Li","doi":"10.1016/j.rvsc.2025.105815","DOIUrl":"10.1016/j.rvsc.2025.105815","url":null,"abstract":"<div><div>This study investigates the proteomic characteristics of goat serum during pregnancy by analyzing differentially abundant proteins (DAB) related to pregnancy maintenance. A total of 102 healthy goats underwent estrus synchronization and artificial insemination (AI), with serum samples collected on days 19, 28, 35, and 50 post-AI. Samples were categorized into five groups: A1 (Day 19), A2 (Day 28), A3 (Day 35), A4 (Day 50), and B (non-pregnant), and each group had five biological replicates. Using data-independent acquisition (DIA) technology, 19,740 proteins were identified, and 2201 DAB were recognized based on fold change criteria (FC ≥ 1.5 or FC ≤ 0.66) and <em>P</em> &lt; 0.05. Bioinformatics analyses revealed that these proteins were enriched in immune response, metabolism, cell proliferation, and reproductive pathways. Notably, proteins such as VKORC1L1, PSTPIP2, and CD40LG exhibited continuous upregulation in early pregnancy, indicating their roles in embryonic development and immune regulation. These findings offer valuable insights into the mechanisms of pregnancy maintenance in goats.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105815"},"PeriodicalIF":2.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}