Research in veterinary science最新文献

{"title":"Efficacy of GPE− strain live attenuated vaccine and CP7_E2alf strain recombinant live vaccine (marker vaccine) against Japanese epidemic classical swine fever virus isolated in 2019 and DIVA discrimination ability of the marker vaccine","authors":"Maiko Yamashita ,&nbsp;Shoko Iwamoto ,&nbsp;Mariko Ochiai ,&nbsp;Kasumi Sudo ,&nbsp;Takao Nagasaka ,&nbsp;Akito Saito ,&nbsp;Takashi Kozasa ,&nbsp;Tsutomu Omatsu ,&nbsp;Tetsuya Mizutani ,&nbsp;Kinya Yamamoto","doi":"10.1016/j.rvsc.2024.105484","DOIUrl":"10.1016/j.rvsc.2024.105484","url":null,"abstract":"<div><div>Classical swine fever (CSF) re-emerged in Japan in 2018, with the epidemic virus identified as genotype 2.1, which is moderately virulent and more difficult to detect and control than the highly virulent strain. Domestic pigs were administered with GPE⁻ strain live attenuated vaccine (GPE⁻ vaccine) for outbreak management. CP7_E2alf strain recombinant live vaccine (marker vaccine), approved for differentiation of infected from vaccinated animals (DIVA), was considered optional for obtaining CSF-free country certification issued by the World Organization for Animal Health. This study aimed to assess the efficacy of both vaccines in pigs through experimental challenge tests and evaluate the DIVA ability of the marker vaccine using two enzyme-linked immunosorbent assay (ELISA) antibody detection kits. Results showed that both GPE⁻ and marker vaccines were effective against the Japanese epidemic strain; however, the ability of the ELISA antibody detection kits to discriminate the marker vaccine was limited. Therefore, to achieve CSF-free certification using vaccines with DIVA functionality, alternative detection methods and enhancement of the sensitivity and specificity of ELISA kits are needed.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105484"},"PeriodicalIF":2.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of the Cuvier's beaked whale (Ziphius cavirostris) myogenic cell line","authors":"Antonella Peruffo ,&nbsp;Irene Bassan ,&nbsp;Alice Gonella ,&nbsp;Lisa Maccatrozzo ,&nbsp;Cristina Otero-Sabio ,&nbsp;Leopoldo Iannuzzi ,&nbsp;Angela Perucatti ,&nbsp;Ramona Pistucci ,&nbsp;Marta Giacomello ,&nbsp;Cinzia Centelleghe","doi":"10.1016/j.rvsc.2024.105471","DOIUrl":"10.1016/j.rvsc.2024.105471","url":null,"abstract":"<div><div>In this study we present the first <em>in vitro</em> model based on a muscle-derived cell line from a male Cuvier's Beaked whale stranded along the Italian coastline.</div><div>In the myogenic cell line at T0 stage we performed the growth rate assay, the karyotyping, the CBA chromosome banding, G banding, nucleolar organizer regions and telomere analysis and immunocytochemical analysis. The cell characterization at the T2 and T4 stage included: assessment of fusion index, the ultrastructural analysis and immunocytochemical analysis.</div><div>The population doubling time was determined to be ∼54 h. The cell fusion index at the T2 stage was 8.2 +/− 5 %, at the T4 stage increased at index 28 +/− 10 %. The karyotype analysis revealed a 2n = 42, XY, two pairs of nucleolus organizer regions (NORs), characteristic CBA-banding and PNA-telomeric regions by FISH-mapping.</div><div>The immunocytochemical results revealed that we generated a heterogeneous population of myogenic cells. A cell population express β-actin, myosin and vimentin and a sub-population of cells was desmin-positive.</div><div>In details, at the T0 stage, both cell populations were undifferentiated mononucleated myoblast. At the T2 and T4 stage, the cells were capable of fusion in elongated multinucleated myotubes, probably resulting from the fusion of the myoblasts.</div><div>This Cuvier's Beaked whale cell line, represents a new opportunity to better understand the physiological features of the cetacean <em>Ziphius cavirostris</em> myogenic cells. The possibility of setting up culture conditions that mimic the <em>in vivo</em> microenvironment, strengthen the importance of <em>in vitro</em> models for toxicological studies investigating water pollutants effects on cells.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105471"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus and methicillin-resistant staphylococci and mammaliicocci in the bulk tank milk of dairy cows from a livestock-dense area in northern Italy","authors":"Sara Fusar Poli ,&nbsp;Clara Locatelli ,&nbsp;Valentina Monistero ,&nbsp;Gustavo Freu ,&nbsp;Paola Cremonesi ,&nbsp;Bianca Castiglioni ,&nbsp;Cristina Lecchi ,&nbsp;Carla Maria Longheu ,&nbsp;Sebastiana Tola ,&nbsp;Alessandro Guaraglia ,&nbsp;Carolina Allievi ,&nbsp;Luca Villa ,&nbsp;Maria Teresa Manfredi ,&nbsp;Maria Filippa Addis","doi":"10.1016/j.rvsc.2024.105482","DOIUrl":"10.1016/j.rvsc.2024.105482","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is the main etiologic agent of contagious dairy cow mastitis, while non-<em>aureus</em> staphylococci and mammaliicocci (NASM) are the bacteria most frequently isolated from milk. Beyond their impact on animal health, NASM can harbor antimicrobial resistance (AMR) genes with potential for bidirectional transfer with <em>S. aureus</em>, and methicillin-resistant (MR) staphylococci (MRS) can raise significant One Health concerns. In our study, we evaluated the prevalence and characteristics of MRS in the bulk tank milk (BTM) of 88 dairy farms in the livestock-dense province of Lodi, Lombardy, northern Italy. <em>S. aureus</em> was isolated from 32.95 % of BTM samples, with the Ribosomal Spacer PCR (RS-PCR) genotype B being the most prevalent, identified in 37.93 % of <em>S. aureus</em> positive farms. All isolates carried the <em>ica</em> genes (<em>ica</em>A, <em>ica</em>B, <em>ica</em>C, <em>ica</em>D) indicating the potential to produce biofilm. MRS were isolated in 56.81 % of farms. According to MALDI-TOF MS analysis, the most prevalent MR species included <em>S. epidermidis</em> (MRSE, 35.59 %) followed by <em>S. aureus</em> (MRSA, 18.64 %), <em>M. sciuri</em> (15.25 %), <em>S. saprophyticus</em> (11.86 %), <em>S. borealis</em> (6.78 %), <em>S. haemolyticus</em> (5.08 %), <em>M. fleurettii</em>, (3.39 %), <em>S. cohnii</em>, and <em>S. pettenkoferi</em> (1.70 % each). Most MR isolates carried the <em>mec</em>A gene, while none carried <em>mec</em>C. The staphylococcal cassette chromosome <em>mec</em> (SCC<em>mec</em>) was predominantly type V in MRSA (45.45 %) and type IV in MRSE (61.90 %). Given their relevance to One Health, monitoring AMR in all staphylococci and mammaliicocci isolated from milk is essential for understanding the prevalence, characteristics, and transmission dynamics of MR gene pools within dairy herds.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105482"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the quality of life-enhancing effect of allogeneic feline adipose mesenchymal stem cells in cats with osteoarthritis: A pilot study","authors":"Kosuke Mitani ,&nbsp;Yuki Ito ,&nbsp;Yukio Takene ,&nbsp;Toshio Inaba","doi":"10.1016/j.rvsc.2024.105470","DOIUrl":"10.1016/j.rvsc.2024.105470","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a progressive degenerative disease in older cats, and often leads to decreased quality of life (QOL). Mesenchymal stem cells (MSCs) have been used in novel therapies for inflammatory diseases. We aimed to evaluate quantitatively allogeneic adipose-derived MSC (ADSC) therapy in cats with naturally occurring OA, based on QOL assessment resources. To characterize the in vitro properties of ADSCs, we estimated ADSCs from four healthy cats with respect to morphology, differentiation potential, and immunomodulatory potential. Six cats with OA were administered a single intravenous injection of allogeneic ADSCs. Based on the feline musculoskeletal pain index (FMPI), the outcome measure was QOL. The cultured cells were adherent, exhibited a spindle shape without becoming flattened or large, and maintained doubling time until passage 5. After induction, the cells had osteogenic, adipogenic, and chondrogenic phenotypes. These cells expressed CD44 and CD90 and lacked expression of CD14 and CD45, had significantly suppressed the production of interferon -ɤ released from mitogen-stimulated lymphocytes (<em>P</em> &lt; 0.05). The FMPI of all cats with OA significantly increased one month after ADSC therapy (<em>P</em> &lt; 0.05). No adverse effects associated with ADSC administration were observed during follow-up in any of the cats. In conclusion, ADSC therapy with immunomodulatory potential could have beneficial effects on the QOL in cats with OA. Further research is necessary to carry out larger studies of the effectiveness of ADSC therapy.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105470"},"PeriodicalIF":2.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota composition of an autochthonous Krškopolje pig breed reared in two different organic production systems","authors":"Bojan Papić ,&nbsp;Tim Šteferl ,&nbsp;Jan Plut ,&nbsp;Marina Štukelj","doi":"10.1016/j.rvsc.2024.105449","DOIUrl":"10.1016/j.rvsc.2024.105449","url":null,"abstract":"<div><div>It has been shown that different production systems (conventional vs. pasture-raised pigs) and co-rearing of pigs with other livestock influence the gut microbiota composition in pigs. In this study, two independent trials were conducted to investigate the 16S fecal microbiota of the autochthonous Krškopolje pig. In each trial, three study groups were analyzed: (<em>i</em>) pasture-raised pigs cohabiting with small ruminants (group L1, farm 1), (<em>ii</em>) indoor-raised pigs without contact with other farm animals (group L2, farm 2) and fed the same commercial organic feed as the other two groups, and (<em>iii</em>) pasture-raised pigs cohabiting with cattle (group L3, farm 2). The pigs were sampled four times during the grower-finisher period, corresponding to the different seasons. A total of 18 and 22 pigs were included in trials 1 and 2, respectively. Alpha diversity was comparable between the study groups and the pigs of different age categories. The two predominant bacterial phyla in all three groups were <em>Bacteroidota</em> and <em>Firmicutes</em>. Significant differences in microbiota composition were found between pigs of different age categories in both trials (nonparametric MANOVA, <em>p</em> &lt; 0.008). The microbiota composition of pigs from group L1 was significantly different from that of pigs from groups L2 and L3, whereas groups L2 and L3 tended to be more similar in both trials. The present results indicate that the trial and the farm of origin have a significant influence on the pig gut microbiota, and that their influence is more pronounced than that of the housing system.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105449"},"PeriodicalIF":2.2,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paeonol attenuates LPS-induced inflammatory injury in mastitis by regulating the MAPK and Nrf2 signaling pathways","authors":"Ming Yang ,&nbsp;Jiaze Han ,&nbsp;Zhengui Yan ,&nbsp;Ke Li","doi":"10.1016/j.rvsc.2024.105481","DOIUrl":"10.1016/j.rvsc.2024.105481","url":null,"abstract":"<div><div>Mastitis is an inflammatory udder disease that causes important economic losses in the animal breeding and dairy product industries. While antibiotics have contributed to disease control, their use presents risks due to drug residues in livestock products that can potentially threaten human health. Paeonol, an active ingredient extracted from the dried root bark of <em>paeonia suffruticosa</em>, exhibits various pharmacological effects, including anti-inflammatory and antibacterial properties, but its role in mastitis remains unclear. In this study, we first evaluated the effects of paeonol on LPS-induced mastitis in mice and further explored the potential anti-inflammatory mechanism of paeonol on bovine mammary epithelial cells (BMECs). The results showed that paeonol alleviated LPS-induced mouse mastitis by inhibiting the infiltration of inflammatory cells and the release of inflammatory factors. Cellular assays further demonstrated that paeonol reduced apoptosis and inhibited inflammation and oxidative stress injury by mechanisms involving activation of Nrf2 signaling and inhibition of MAPK signaling in BMECs. Collectively, our results suggest that paeonol holds promise as a therapeutic agent for alleviating LPS-induced mastitis injury while providing novel insights into potential drug targets for mastitis treatment.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105481"},"PeriodicalIF":2.2,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral red flags for optimizing castration-induced acute pain diagnosis in cattle","authors":"Gustavo Venâncio da Silva ,&nbsp;Rubia Mitalli Tomacheuski ,&nbsp;Flavia Augusta de Oliveira ,&nbsp;Monique Danielle Pairis-Garcia ,&nbsp;Stelio Pacca Loureiro Luna ,&nbsp;Pedro Henrique Esteves Trindade","doi":"10.1016/j.rvsc.2024.105468","DOIUrl":"10.1016/j.rvsc.2024.105468","url":null,"abstract":"<div><div>Unesp-Botucatu Cattle Pain Scale (UCAPS) is widely used in experimental settings, however the high number of UCAPS behaviors might represent a barrier to its implementation in the farm's or hospital's routine. We aimed to identify a smaller combination of UCAPS behaviors that could be used as behavioral red flags for optimizing the acute pain diagnosis in cattle. We hypothesize that a specific set of UCAPS behaviors might be used as behavioral red flags for pain. This would represent a quick and simple pain evaluation and might optimize the acute pain assessment in large-scale systems. Data from two previous studies regarding UCAPS assessments before (pain free condition) and after (painful condition) surgical castration of 59 male cattle was used. We fitted a decision tree, resulting in a logic with two behaviors that we used as behavioral red flags. The logic adapted from the decision tree considered the painful diagnosis when the Activity was scored 2. When Activity was scored less than 2, but Locomotion was scored 1 or 2, the diagnosis was also considered positive for pain. When Activity was below 2 and Locomotion was 0, the diagnosis was considered free pain. Behavioral red flags had an area under the curve of 95.95 % for predicting UCAPS diagnosis and 94.13 % for predicting overall pain free and painful conditions. In conclusion, behaviors in the decision tree logic can work as behavioral red flags for optimizing the acute pain diagnosis in cattle, as a quick assessment in large-scale systems.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105468"},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA H19 improves mesenchymal characteristics of buffalo (Bubalus bubalis) bone marrow-derived mesenchymal stem cells under hypoxic conditions","authors":"Lulu Wang ,&nbsp;Jun Zhang ,&nbsp;Sheng Ye ,&nbsp;Fenghua Lu","doi":"10.1016/j.rvsc.2024.105461","DOIUrl":"10.1016/j.rvsc.2024.105461","url":null,"abstract":"<div><div>As adult stem cells with various advantages, Bone marrow-derived mesenchymal stem cells (BMSCs) are valuable resources for veterinary treatment and animal reproduction. Previous studies have shown that hypoxia can induce epithelial-mesenchymal transition (EMT) and improve mesenchymal characteristics of BMSCs in vitro culture. However, the mechanism by which hypoxia improves the interstitial characteristics of buffalo BMSCs (bBMSCs) remains unclear. In this study, the effects of hypoxia on the mesenchymal characteristics of bBMSCs and the expression level of lncRNA H19 were examined, and then the effects of lncRNA H19 on maintaining the mesenchymal characteristics of bBMSCs under hypoxic culture conditions (5 % oxygen) as well as its mechanism also were explored, so as to further understand the molecular mechanism of mesenchymal characteristics maintenance of bBMSCs. The results showed that hypoxic culture conditions promoted EMT of bBMSCs, with lncRNA H19 expression up-regulated. When lncRNA H19 was knocked down in hypoxia, the expression level of Vimentin was down-regulated, the expression level of E-Cadherin was up-regulated, and EMT was inhibited. Meanwhile, the genes (p-PI3K and p-AKT1) involved in PI3K/AKT signaling pathway were inhibited by lncRNA H19 Knockdown. IGF-1 (10 ng/mL), an activator of PI3K/AKT signaling pathway, was added to rescued the inhibition of PI3K/AKT signaling pathway caused by lncRNA H19 Knockdown, with the effects of lncRNA H19 on EMT related genes also partially reversed. These findings not only provide theoretical guidance to elucidate the detailed regulation mechanism of hypoxia on mesenchymal nature maintenance of bBMSCs, but also provide positive support to further establish the stable in vitro culture system of bBMSCs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105461"},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation, in vitro evaluation and therapeutic effect of chitosan coated pluronic F127 micelles containing miltefosine for the treatment of visceral leishmaniasis in domestic dogs naturally infected by Leishmania infantum","authors":"Zahra Alizadeh ,&nbsp;Mehdi Mohebali ,&nbsp;Behzad Aghabarari ,&nbsp;Zabihollah Zarei ,&nbsp;Gholamreza Hassanpour ,&nbsp;Seyed Ebrahim Eskandari ,&nbsp;Mehdi Parsaei ,&nbsp;Shahram Jamshidi ,&nbsp;Abbas Rahimi Foroushani ,&nbsp;Hossein Keshavarz ,&nbsp;Behnaz Akhoundi ,&nbsp;Zahra Heidari ,&nbsp;Fatemeh Mohebali ,&nbsp;Seyedeh Parastoo Yasini ,&nbsp;Akram Hassanpour","doi":"10.1016/j.rvsc.2024.105467","DOIUrl":"10.1016/j.rvsc.2024.105467","url":null,"abstract":"<div><div>There are a limited number of chemotherapeutic agents for the treatment canine visceral leishmaniasis (CVL). This study aimed to formulate and assess micellar formulation of miltefosine (MTF) to increase antileishmanial therapeutic effects in dogs naturally infected by <em>Leishmania (L) infantum</em> for the first time<em>.</em> MTF loaded pluronic F127 (PF/MTF) micelles were formulated and coated with chitosan (Cs/PF/MTF). Toxicity of compounds was evaluated. The <em>in vitro</em> and <em>in vivo</em> antileishmanial effect of Cs/PF/MTF was examined in L. <em>infantum</em> promastigotes and amastigotes as well as naturally infected dogs by L. <em>infantum</em>. <em>In-vitro</em> efficacy studies in promastigotes and amastigotes demonstrated that inhibitory concentration (IC50) of Cs/PF/MTF was significantly lower in comparison to miltefosine. <em>In vivo</em> data showed that Cs/PF/MTF is more effective against CVL compared with miltefosine alone. The result of this study indicated that intravenous injection of new formulation of Cs/PF/MTF was tolerated and treated on L. <em>infantum</em> naturally infected dogs with the dosages of 170 μg/kg daily for 28 days.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105467"},"PeriodicalIF":2.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of point-of-care immunodiagnostic test for Taenia solium cysticercosis in pigs","authors":"Haranahally Vasanthachar Manjunathachar ,&nbsp;Buddhi Chandrasekaran Saravanan ,&nbsp;Chitra Joshi ,&nbsp;Aquil Mohmad ,&nbsp;M. Arvind ,&nbsp;Siju Susan Jacob ,&nbsp;M. Sankar","doi":"10.1016/j.rvsc.2024.105466","DOIUrl":"10.1016/j.rvsc.2024.105466","url":null,"abstract":"<div><div>Cysticercosis, caused by the larval stage of <em>Taenia solium</em>, is a major zoonotic parasitic disease with a global impact, heightened by immigration and transboundary activities. Recognizing its importance, the World Health Organization has prioritized eliminating taeniasis/cysticercosis by 2030, designating it as a priority neglected tropical disease (NTD). Accurate diagnosis is essential for effective disease control, yet traditional methods often fall short due to low sensitivity of the test and the need for specialized laboratory setups. This study focuses on developing a recombinant Ag2 (rAg2)-based dot blot assay to detect <em>T. solium</em> cysticercosis in pigs. The rAg2 antigen was successfully expressed and purified, with immunoreactivity confirmed against sera from naturally infected pigs. The dot blot test evaluated against serological gold-standard enzyme-linked immunotransfer blot (EITB) assay, demonstrated preliminary laboratory sensitivity (89 %, 95 % CI: 68.6–97.1) and specificity (100 %, 95 % CI: 97.6 - 100), highlighting its potential as a reliable alternative diagnostic method. Cost-effectiveness and minimal laboratory requirements make it particularly suited for field use in resource-limited settings.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105466"},"PeriodicalIF":2.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}