Research in veterinary science最新文献

{"title":"Integrated analysis of transcriptome and metabolome reveal the complex molecular regulatory network of chicken house PM2.5 induced lung injury","authors":"Bo Wu ,&nbsp;Wendan Zheng ,&nbsp;Yu Bai ,&nbsp;Hongrui Huang ,&nbsp;Tongjie Chai","doi":"10.1016/j.rvsc.2025.106039","DOIUrl":"10.1016/j.rvsc.2025.106039","url":null,"abstract":"<div><div>Fine particulate matter (PM2.5) in poultry houses is the main component of air pollution. Long-term exposure to high concentrations of PM2.5 could easily damage the respiratory system of animals. In this study, we first investigated the changes in lung tissue morphology and serum cytokines in SPF chickens after exposure to poultry house PM2.5 and further conduct a combined analysis of its lung transcriptome and metabolome. The results showed that chicken exposed to PM2.5 led to decrease in body weight and cause inflammatory damage such as pulmonary fibrosis in chickens. It also significantly upregulated the expression levels of serum cytokines IL-1β, IL-6, TLR-2, TLR-4, IFN-α, TGF-β and CCL2. Additionally, it affected Arachidonic acid metabolism, Ferroptosis, and mTOR signaling pathways, disrupting metabolic processes and the expression of genes related to pulmonary inflammation. In addition, we validated the results of the multi-omics combined analysis and found that the interaction between the differential metabolite Arachidonic acid and the three differentially expressed genes LTA4H, FTL and PLA2G4A led to pulmonary inflammatory damage and metabolic disorders. The above results indicate that the interaction between pulmonary inflammation-related genes and lung metabolites may play a crucial role in the process of PM2.5-induced lung injury in chickens.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106039"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoledronate-induced activation of γδ T cells is associated with NK cell activation and reduced parasite burden in the cecum of Eimeria tenella-infected chicks","authors":"Quan Viet Le ,&nbsp;Makoto Matsubayashi ,&nbsp;Toshimitsu Hatabu","doi":"10.1016/j.rvsc.2025.106030","DOIUrl":"10.1016/j.rvsc.2025.106030","url":null,"abstract":"<div><div>This study aimed to elucidate the role of γδ T cells in chicks infected with <em>Eimeria tenella</em>. Zoledronate was administered in vivo to activate γδ T cells. White Leghorn chicks were divided into four groups: zoledronate-treated (ZOL), infected control (CT), uninfected zoledronate-treated (UN/ZOL), and uninfected control (UN). Chicks in the CT and ZOL groups were orally inoculated with sporulated <em>E. tenella</em> oocysts (1 × 10^4 oocysts/chick) at 14 days of age. Fecal samples were collected between 4 and 15 days post-infection (dpi) to assess oocyst shedding. Cecal samples were obtained at 5 dpi for histopathological examination and gene expression analysis. Lesion and parasite burden scores were significantly reduced in the ZOL group compared with the CT group. Expression levels of IL-17 A, IL-21, and IFN-γ were significantly higher in the ZOL group, whereas IL-13 and PGES expression was significantly higher in the CT group. These findings indicate that activation of γδ T cells alleviates cecal tissue damage caused by <em>E. tenella</em> infection. Moreover, they suggest that NK cells activated by IL-21 may play a role in parasite control and contribute to the establishment of the local immune environment, but the effect of parasite control by IL-21-induced NK cell activation requires further research. Future studies should also explore whether γδ T cell activation and IL-21-mediated immunity are associated with mechanisms of parasite control.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106030"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shannon entropy as a stable diffusion tensor parameter for evaluating normal spinal cord regions in dogs","authors":"Yoshimichi Goda ,&nbsp;Yuko Mizutani ,&nbsp;Mizuki Hirayama ,&nbsp;Taketoshi Asanuma ,&nbsp;Hiroyuki Satoh","doi":"10.1016/j.rvsc.2025.106036","DOIUrl":"10.1016/j.rvsc.2025.106036","url":null,"abstract":"<div><div>Diffusion tensor imaging (DTI) has been used in veterinary medicine for cerebral infarctions and degenerative diseases in recent years. Defining normal values for the apparent diffusion coefficient (ADC) and fractional anisotropy (FA) to detect lesions is challenging because these values depend on anatomical differences in the spinal cord, magnetic resonance imaging (MRI) machine model, magnetic field strength, and measurement protocol. Shannon entropy is the average amount of information and is a measure of the variability or complexity, of distributed numbers. The purpose of this study is to evaluate whether Shannon entropy can serve as a useful new indicator for interpreting the structural integrity of the canine spinal cord. We used six beagle dogs with no spinal cord disease, as confirmed by neurological examination and magnetic resonance imaging. We calculated the respective values for the cervical and thoracolumbar spinal cord under the 3 T magnetic field. The ADC and FA values (mean ± standard error) of the cervical spinal cord were 1.11 ± 0.05 × 10⁻³ mm²/s and 0.64 ± 0.02. The thoracolumbar spinal cord had values of 1.16 ± 0.04 × 10⁻³ mm²/s and 0.58 ± 0.02. The ADC and FA values of the spinal cord reflected anatomical differences, and no fixed normal values were observed for the entire spinal cord. Shannon entropy was 5.67 ± 0.45. No significant differences were observed in the values at any intervertebral level. These results suggest that Shannon entropy is more useful than the ADC and FA values. Shannon entropy may be able to differentiate between normal tissue and lesions, independent of various MRI conditions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106036"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative evaluation and multivariate analysis-based ranking of factors influencing blood proviral load in breeding cattle infected with bovine leukemia virus","authors":"Yuki Fujii ,&nbsp;Masaki Maezawa ,&nbsp;Shun Ishizuka ,&nbsp;Yuki Ishida ,&nbsp;Shoko Ooya ,&nbsp;Yuki Kashima ,&nbsp;Kaoru Yamashita ,&nbsp;Nahoko Kawanishi ,&nbsp;Masataka Akagami ,&nbsp;Yoshiko Ootani ,&nbsp;Akiyuki Watanabe ,&nbsp;Satoshi Takahashi ,&nbsp;Masato Ishii ,&nbsp;Junko Kawakami ,&nbsp;Yuri Fujimoto ,&nbsp;Hisashi Inokuma","doi":"10.1016/j.rvsc.2025.105995","DOIUrl":"10.1016/j.rvsc.2025.105995","url":null,"abstract":"<div><div>Bovine leukemia virus (BLV) infection in cattle results in variable proviral load (PVL), a key indicator of viral transmission and disease progression. However, the relative contributions of host and viral factors to PVL are not fully understood. The present study evaluated four potential determinants of PVL in BLV-infected cattle: two host-related factors (BoLA-DRB3 alleles and age) and two viral factors (Tax amino acid 233 (TAX-233) and 5’LTR nucleotide 175 (LTR-175)) in 1673 Japanese Black (JB) and 979 Holstein Friesian (HF) breeding cows. The contribution of each factor to PVL was quantified using Quantification Theory Type I. BoLA-DRB3 and age accounted for the greatest variation in both JB (55.1 % and 32.4 %) and HF (65.6 % and 18.2 %) cattle, while TAX-233 and LTR-175 made smaller contributions. Notably, the L233 and 175C variants—linked to increased tumorigenicity and transmissibility—were significantly associated with higher PVL in HF but not JB cattle, suggesting breed-specific viral effects. Despite their clinical relevance, these viral markers explained PVL variation to a lesser degree than host factors. These findings underscore the limitations of PVL-based culling strategies, since low-PVL animals may still harbor high-risk viral genotypes. We recommend the integration of viral genotyping into risk assessment frameworks to improve control measures. Although our multivariable models explained only a modest portion of PVL variance (R² = 5.5–11.7 %), the independent contributions of each factor were confirmed. Future studies incorporating additional host, viral, and environmental variables will be essential to refine predictive models for BLV pathogenesis and transmission.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 105995"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal distribution and genetic characteristics of porcine reproductive and respiratory syndrome virus-2 lineage 1 in Japan from 2020 to 2023","authors":"Ryosuke Takai ,&nbsp;Tetsuo Sato ,&nbsp;Yoriko Yonezawa ,&nbsp;Nobuyuki Tsutsumi ,&nbsp;Ryohei Nukui ,&nbsp;Katsuaki Sugiura ,&nbsp;Osamu Taira","doi":"10.1016/j.rvsc.2025.106025","DOIUrl":"10.1016/j.rvsc.2025.106025","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a major swine disease globally. This study analyzed 1207 PRRSV sequences (2020-2023) detected through routine diagnostic submissions in Japan using the standardized open reading frame 5-based lineage classification system. Phylogenetic analysis identified six lineages in Japan: L1, L4, L5, L8, L9, and L11. Notably, the prevalence of L1 showed a marked increase during the study period. L1 strains were predominantly detected in the Kanto and Kyushu regions. In Kanto, L1B was predominant (80 %), whereas Kyushu exhibited greater sublineage diversity (L1A, L1B, L1E, and L1I). Pairwise nucleotide identity analysis revealed high genetic heterogeneity within the L1 population (mean identity 90.1 %), while L5 and L8 populations were highly homogenous (97.9 % and 98.3 %, respectively) and showed high sequence identity (&gt;95 %) to commercial vaccine strains. This study presents the first nationwide molecular epidemiological analysis of PRRSV in Japan based on the global lineage system. The findings highlight Japan's distinct L1 sublineage dynamics (e.g., L1B dominance) compared to global trends (e.g., L1C.5 in the US and NADC30/34-like strains in China and Korea), offering critical insights for informing effective PRRSV control strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106025"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145768955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary supplementation of an oleuropein-enriched olive leaf extract on performance, carcass characteristics, intestinal health, foot pad parameters and meat quality of broilers","authors":"Konstantina Vasilopoulou ,&nbsp;Georgios A. Papadopoulos ,&nbsp;Styliani Lioliopoulou ,&nbsp;Leandros Skaltsounis ,&nbsp;Panagiotis Stathopoulos ,&nbsp;Ioanna Stylianaki ,&nbsp;Dimitrios Galamatis ,&nbsp;Vasileios Tsiouris ,&nbsp;Georgios Arsenos ,&nbsp;Ilias Giannenas","doi":"10.1016/j.rvsc.2025.105990","DOIUrl":"10.1016/j.rvsc.2025.105990","url":null,"abstract":"<div><div>Incorporating agricultural by-products into animal diets represents a sustainable strategy to enhance animal health and product quality. Olive leaves, rich in bioactive compounds such as oleuropein, exhibit notable antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluated the effects of dietary supplementation with an environmentally friendly, oleuropein-enriched OLE (OLE) on broiler chickens. A total of 480 day-old chicks were randomly assigned to four groups: a control diet - CF (T1), CF + 1 % OLE (T2), CF + 2.5 % OLE (T3), and CF + 0.1 % encapsulated oregano oil (T4, positive control). Over 42 days, growth performance, carcass characteristics, intestinal morphology, foot pad health, and meat quality were assessed. While T4 showed superior growth performance, the 1 % OLE diet significantly improved intestinal morphology and meat quality. A dose-dependent yellow pigmentation of the foot pad was observed in OLE-treated groups. Additionally, breast tenderness and darker thigh coloration improved in OLE-fed broilers. These findings suggest that 1 % OLE can be a viable natural additive to support gut health and meat quality in broilers, with comparable antioxidant effects to oregano oil and no adverse outcomes.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 105990"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145668414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot study: Effects of whole-body vibrational therapy on equine thoracolumbar epaxial muscle response to pressure algometry","authors":"Riley Leibeck ,&nbsp;Jeffrey Lehman ,&nbsp;Sheri Birmingham","doi":"10.1016/j.rvsc.2025.106027","DOIUrl":"10.1016/j.rvsc.2025.106027","url":null,"abstract":"<div><h3>Background</h3><div>Whole-body vibrational therapy (WBV) is well-studied in people, but there is a paucity of research regarding its effect on horses. It is suggested that the use of WBV in horses increases performance, aids in recovery, and stimulates nerves, bones, and muscles in ways that normal exercise does not.</div></div><div><h3>Objective</h3><div>The purpose of this pilot study was to determine if frequent WBV therapy sessions affect mechanical nociceptive thresholds based on pressure algometry in horses without clinical signs of lameness or back pain.</div></div><div><h3>Methods</h3><div>The mechanical nociceptive thresholds (MNTs) were measured for 5 horses at 8 points along their thoracolumbar epaxial muscles once every 7 days for 8 weeks. Baseline MNT scores were established during a control period with no vibration therapy treatment over the first 4 weeks. During the second 4-week period, horses received 5, 30-min vibration therapy sessions per week.</div></div><div><h3>Results</h3><div>Despite previous research indicating that WBV increases epaxial muscle symmetry in horses, no significant difference was noted between the horses' mean MNTs across all 8 sites during the baseline and treatment periods. Upon further investigation of the change in sensitivity at the individual sites across all subjects, a statistically significant (<em>p</em> &lt; 0.05) increase in MNT (0.49–0.62 kgf) was observed at 4 out of the 8 sites.</div></div><div><h3>Conclusion</h3><div>While an increase in mean MNTs was not observed, this study found that WBV may increase MNTs at individual sites along the thoracolumbar epaxial musculature in horses. Further investigation is required to assess whether this difference is biologically meaningful and repeatable.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106027"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145775240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, physiological responses, and histopathological changes of Oreochromis niloticus fingerlings exposed to aqueous gold nanoparticles (AuNs) at high stocking density","authors":"Randa Y. Thabet ,&nbsp;Sameh M. Farouk ,&nbsp;Shahad M. Elkim ,&nbsp;Ashraf Y. El-Dakar ,&nbsp;Sherifa H. Ahmed ,&nbsp;Dalia S. Hamza ,&nbsp;Omar Abdel-hamed Ahmed-Farid ,&nbsp;Mohamed F. Abdel-Aziz","doi":"10.1016/j.rvsc.2025.105991","DOIUrl":"10.1016/j.rvsc.2025.105991","url":null,"abstract":"<div><div>Despite the ubiquitous usage of nanoparticles (Ns), there are still significant gaps of knowledge regarding the impact of gold nanoparticles (AuNs) on one of the economically important fish species as Nile tilapia (<em>Oreochromis niloticus</em>). Therefore, this study was conducted to investigate the impact of gold nanoparticles (AuNs) on the growth performance, serum biochemistry, antioxidant enzymes levels, digestive enzyme activity, and histopathological changes of different studied tissues such as gills and liver in Nile tilapia (<em>Oreochromis niloticus</em>) that exposed to various concentrations of AuNs (0, 5, 10, 15 and 20 μgL⁻¹) respectively as aqueous exposure, particularly in the context of heavy metal exposure. Fish were randomly distributed in 15 glass aquariums (70 L) at a stocking rate of 20 fish/aquarium (treatment = 3 aquariums: 60 fish). Fish were within an initial average weight of 4.02 g ± 0.27 and fed a commercial diet containing 30 % crude protein (CP) at a feeding rate of 4 % of biomass. Results showed improvements in growth with AuNs exposure, but the survival rate decreased with increasing concentrations of AuNs exposure. The 20 μg L⁻¹ AuNs group exhibited the highest specific growth rate (SGR: 3.45 %/day), representing a 17.7 %/day increase compared to the control (2.93 %/day), alongside a 44.7 % reduction in survival rate (52.55 % vs. 95 % in controls). Intermediate concentrations (e.g., 5 or 10 μg L-1 AuNs) improved SGR (3.16 and 3.23 %/day) with less severe survival impacts (92.5 and 75 %, respectively).</div><div>Also, increasing doses of AuNs led to negative effects on physiological status and histological study of the liver and gills. Fish that were exposed to ≤5 μg L⁻¹ exhibited the highest survival rate and enhanced resistance to heavy-metal challenge. It can be recommended that aqueous exposure to gold nanoparticles requires further studies, and it appears that doses less than 5 μm may have positive effects on growth and immune functions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 105991"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic use of enzyme-linked immunosorbent assay as a complementary tool for the diagnosis of bovine tuberculosis","authors":"C. Camussone ,&nbsp;R.D. Moyano ,&nbsp;M.N. Alonso ,&nbsp;J. Cicotello ,&nbsp;A. Peña ,&nbsp;A.I. Molineri ,&nbsp;D.M. Astesana ,&nbsp;G. Suárez-Archilla ,&nbsp;F. Tello D'Elia ,&nbsp;D. Aliprandi ,&nbsp;N.M. Welschen ,&nbsp;M.L. Signorini ,&nbsp;M.V. Zbrun","doi":"10.1016/j.rvsc.2025.106017","DOIUrl":"10.1016/j.rvsc.2025.106017","url":null,"abstract":"<div><div>Bovine tuberculosis (bTB) is a global chronic disease. Argentina's bTB eradication program consists of the Caudal-Fold Tuberculin test (CFT) and the sacrifice of positive animals as the principal measure. However, despite the implementation of the control program, CFT-positive animals continue to appear in some herds, which generates skepticism about the potential to eradicate tuberculosis. The objective of this work was to evaluate the suitability of an ELISA test for the detection of <em>M. bovis</em> specific antibodies (bTB-ELISA) in CFT-negative animals as a complementary strategy to control bTB. A convenience sampling of dairy farms was conducted through contact with owners, and those who agreed to the research protocol were selected. Two studies were conducted in eight (study 1) and six (study 2) dairy farms. Cows were tested by CFT, and those negative to this test were tested using ELISA in three cycles, one cycle per year during three consecutive years (study 1) and three cycles applied consecutively for 1 year (study 2). The prevalence of bTB-ELISA-positive animals showed a significant reduction towards the third sampling in both strategies. Although the general trend was towards a reduction in bTB-positive animals using CFT, this behavior was not homogeneous among dairy farms, evidencing that intra-farm factors are relevant. The repeated application of the CFT test together with ELISA was a useful strategy for the reduction of CFT-positive animals. It should be noted that this approach must be accompanied by good management practices in the herd to prevent the entry and spread of the disease.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106017"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145725583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical characterization of the Robert Jones bandage under cyclic compression-bending: An in vitro experimental model","authors":"Samuel Larcheveque ,&nbsp;Stéphanie Claeys ,&nbsp;Marc Balligand ,&nbsp;Pierre P. Picavet","doi":"10.1016/j.rvsc.2025.106018","DOIUrl":"10.1016/j.rvsc.2025.106018","url":null,"abstract":"<div><h3>Objective</h3><div>To assess the rigidity of Robert-Jones Bandage (RJB) used to stabilize a standardized in vitro fracture model, under cyclic compression/bending.</div></div><div><h3>Study design</h3><div>In vitro cyclic testing study. Specimens: wooden rods divided into two segments of identical length by a Cardan Joint enclosed in a thick foam tube and supported by a RJB. Procedures: Part 1: incremental loading applied to 6 specimens in successive stages of 50 N from 5 to 150 to 5–300 N with 3000 cycles per stage. Part 2: 9 specimens were loaded at 150 N (5–150 N) during 60,000 cycles.</div></div><div><h3>Statistics</h3><div>The Wilcoxon signed-rank test was used to compare data obtained at 10,000 and 60,000 cycles.</div></div><div><h3>Results</h3><div>In part 1, 6/6 specimens withstood 150 N for 3000 cycles. Three specimens endured an additional 3000 cycles at 200 N, 2 withstood 250 N for another 3000 cycles, and 1 tolerated 300 N. In part 2, 9/9 specimens withstood 150 N for 60,000 cycles, with displacements ranging from 0.102 mm to 0.309 mm (mean 0.179 ± 0.069 mm) and a median rigidity of 897 ± 375 N/mm. No specimen reached the fixed displacement limit of 2 mm after 60,000 cycles. No statistically significant difference was found between the results at 10,000 and 60,000 cycles (<em>p</em> = 0.129).</div></div><div><h3>Conclusion</h3><div>The Robert-Jones Bandage showed consistent rigidity under prolonged cyclic loading in an in vitro fracture model.</div></div><div><h3>Clinical relevance</h3><div>While this in-vitro model cannot replicate the influence of soft tissues, joint motion, or multidirectional physiological forces, the findings suggest that a properly applied Robert Jones bandage may provide short-term mechanical support to a long-bone fracture prior to definitive stabilization. However, clinical applicability should be interpreted with caution given the inherent limitations of the experimental setup.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106018"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}