DIA-based serum proteomics in goat pregnancy: biomarkers & mechanisms

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-18 DOI:10.1016/j.rvsc.2025.105815

Dongliang Xu , Di Wang , Lisheng Zhou, Zhengang Huang, Mingzhi Sun, Xinxin Cao, Jianyu Zhou, Xiaoxiao Gao, Jianning He, Jinshan Zhao, Hegang Li

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Abstract

This study investigates the proteomic characteristics of goat serum during pregnancy by analyzing differentially abundant proteins (DAB) related to pregnancy maintenance. A total of 102 healthy goats underwent estrus synchronization and artificial insemination (AI), with serum samples collected on days 19, 28, 35, and 50 post-AI. Samples were categorized into five groups: A1 (Day 19), A2 (Day 28), A3 (Day 35), A4 (Day 50), and B (non-pregnant), and each group had five biological replicates. Using data-independent acquisition (DIA) technology, 19,740 proteins were identified, and 2201 DAB were recognized based on fold change criteria (FC ≥ 1.5 or FC ≤ 0.66) and P < 0.05. Bioinformatics analyses revealed that these proteins were enriched in immune response, metabolism, cell proliferation, and reproductive pathways. Notably, proteins such as VKORC1L1, PSTPIP2, and CD40LG exhibited continuous upregulation in early pregnancy, indicating their roles in embryonic development and immune regulation. These findings offer valuable insights into the mechanisms of pregnancy maintenance in goats.

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基于dia的山羊妊娠血清蛋白质组学：生物标志物和机制

本研究通过分析与妊娠维持相关的差异丰度蛋白（DAB），探讨山羊妊娠期血清的蛋白质组学特征。选取102只健康山羊进行同期发情和人工授精，分别于人工授精后第19、28、35和50天采集血清样本。将样品分为A1（第19天）、A2（第28天）、A3（第35天）、A4（第50天）和B（未怀孕）5组，每组5个生物重复。使用数据独立采集（DIA）技术，鉴定了19,740个蛋白，并根据折叠变化标准（FC≥1.5或FC≤0.66）和P <；0.05. 生物信息学分析显示，这些蛋白在免疫应答、代谢、细胞增殖和生殖途径中富集。值得注意的是，VKORC1L1、PSTPIP2和CD40LG等蛋白在妊娠早期表现出持续上调，表明它们在胚胎发育和免疫调节中发挥作用。这些发现为山羊维持妊娠的机制提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.