Research in veterinary science最新文献

{"title":"Corrigendum to \"Cadaveric study of the ultrasound-guided inter-transversospinalis plane block in dogs for the cervical epaxial musculature region\" [Res. Vet. Sci. 180 (2024) 105427].","authors":"Manuel Enrique Herrera-Linares, Rocío Fernández-Parra, Carla Zamora-Perarnau, Nuria Fernández-Salesa, Dylan Yaffy, Sandra Sanchis-Mora","doi":"10.1016/j.rvsc.2026.106435","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106435","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":" ","pages":"106435"},"PeriodicalIF":2.1,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Network pharmacology-based identification and validation of novel vaccine candidates against brucellosis\" [Res. Vet. Sci., 211 (2026), 106359].","authors":"Zhiheng Dong, Sha Li, Jiarong Guo, Lidao Bao","doi":"10.1016/j.rvsc.2026.106426","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106426","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":" ","pages":"106426"},"PeriodicalIF":2.1,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed titanium trail plate for repair of diaphyseal fracture of long bones in dogs.","authors":"Luciane Dos Reis Mesquita, Sheila Canevese Rahal, Washington Takashi Kano, André Luiz Jardini Munhoz, Aline Ryie Toma, Maria Jaqueline Mamprim, Guilherme Rech Cassanego","doi":"10.1016/j.rvsc.2026.106440","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106440","url":null,"abstract":"<p><p>The trail plate is a modular system consisting of a trail bar and synthesis modules made of stainless steel, originally developed for humans. This study aimed to evaluate the applicability and effectiveness of a modified trail plate for the treatment of long bone fractures in dogs. Initially, the trail plate was designed by using FEA to set the final model. Three trail plate sizes were designed according to corresponding locking screw diameters. The designs were created using computer-aided design software, and 3D models were produced from the commercial Ti-6Al-4 V powder via direct metal laser sintering (DMLS). The trail plate application included radius/ulna (n = 5), femur (n = 7), and tibia/fibula (n = 7). Radiographic bone healing occurred within 60 days in 100% of radius/ulna fractures, within 90 days in 70% of tibia/fibula fractures, and between 30 and 120 days postoperatively in femoral fractures. Radius/ulna fractures exhibited minimal callus formation, and all animals were free of lameness by 60 days. Femoral fractures showed mostly moderate callus formation, with lameness resolved in 57% of dogs by 60 days. In tibia/fibula fractures, callus ranged from minimal to abundant, with lameness resolved in six dogs by 60 days. Complications occurred in 36.8% of cases, primarily due to trail bar displacement of the module; only one case involved implant failure. In conclusion, this preliminary case series suggests that modified DMLS-manufactured titanium trail plate was clinically feasible for selected canine diaphyseal fractures. However, fixation-related complications indicate that further design optimization and controlled biomechanical studies are necessary before broader recommendation.</p>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"213 ","pages":"106440"},"PeriodicalIF":2.1,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical disc disease in whippets: Results of an online survey involving Italian owners and breeders","authors":"Diletta Dell'Apa ,&nbsp;Ezio Bianchi ,&nbsp;Luigi Auletta ,&nbsp;Mariaelena de Chiara ,&nbsp;Fabiana Micieli ,&nbsp;Francesco Ferrari ,&nbsp;Barbara Lamagna ,&nbsp;Maria Pia Pasolini","doi":"10.1016/j.rvsc.2026.106068","DOIUrl":"10.1016/j.rvsc.2026.106068","url":null,"abstract":"<div><div>Whippet is considered quite a healthy breed. Nonetheless, the perception of some veterinary neurologists is that the occurrence of neurological diseases, especially cervical disc disease (CDD), is frequent in this breed. This study aimed to get information about the prevalence of the most common disorders and conditions affecting the Italian Whippet population, particularly investigating the prevalence of CDD and the perception of the problem by owners and breeders. Owners and breeders included in the Italian Sighthound Club register were invited to answer anonymously an online questionnaire. Descriptive statistics were used for breeder/owner and dog characteristics, and statistical association with the development of CDD were explored. Over 280 Whippets, the overall prevalence of CDD was 7.2% and 29% in Whippets older than 9 years. Whippets older than 9 years were overrepresented in the CDD group (<em>P</em> &lt; 0.0001; O.R. 0.07; 95% CI: 0.02–0.22) compared with the CDD group. These findings confirm the hypothesis that CDD may be a challenging and more prevalent than expected disorder in this breed and health screening programs could be recommended to breeders and owners to assess cervical spine conformation, especially before sporting activity or breeding.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106068"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA-mRNA integrative profiling in canine tubulopapillary mammary carcinoma","authors":"Nayra Cristina Herreira do Valle ,&nbsp;Matheus Fujimura Soares ,&nbsp;Natália Francisco Scaramele ,&nbsp;Amanda de Oliveira Furlan ,&nbsp;Maria Fernanda da Silva Lopes ,&nbsp;Juliana de Souza Felix ,&nbsp;Fernando Vissani Fernandes ,&nbsp;Vinicius Aquiles Gomes Zamboni ,&nbsp;Igor Luiz Salardani Senhorello ,&nbsp;Alexandre Lima de Andrade ,&nbsp;Flavia Lombardi Lopes","doi":"10.1016/j.rvsc.2026.106107","DOIUrl":"10.1016/j.rvsc.2026.106107","url":null,"abstract":"<div><div>Canine mammary tumors (CMTs) are the most common neoplasia in intact female dogs. There are different histological subtypes, which are related to tumor behavior and prognosis. Among them, we highlight the tubulopapillary subtype, one of the most prevalent subtypes in female dogs. Although not the most aggressive subtype overall, it is associated with significantly higher lethality compared to other common subtypes, suggesting an underlying dysregulation of gene expression. However, global expression of RNAs in tubulopapillary CMTs is not available in the literature. Here, we analyzed the differential expression of mRNAs and miRNAs of tubulopapillary CMTs (<em>n</em> = 4) and compared them with normal mammary tissues (n = 4) using microarray. We found 842 downregulated and 385 upregulated mRNAs in the CMTs, which corresponded to the enrichment of 10 biological pathways. Also, we found 30 upregulated miRNAs and one downregulated (cfa-miR-345) in CMTs. Among the differentially expressed mRNAs, we identified 360 mRNAs that were targets of the differentially expressed miRNAs, which enriched 23 biological pathways, with the “Endocytosis” pathway being of particular significance. These findings highlight the complex interactions between mRNAs and miRNAs in tubulopapillary CMTs, thereby improving the understanding of the pathophysiology of this CMT subtype and providing targets for functional validation of biomarkers and/or therapeutics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106107"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S-palmitoylation of the viral Nsp10 by ZDHHC7 restricts PRRSV replication but is counteracted by Nsp2, Nsp4 and GP5","authors":"Huiyuan Jing ,&nbsp;Zhifeng Peng ,&nbsp;Pandeng Zhao ,&nbsp;Erzhen Duan ,&nbsp;Jie Liu ,&nbsp;Xuyong Zhao ,&nbsp;Huawei Li","doi":"10.1016/j.rvsc.2026.106102","DOIUrl":"10.1016/j.rvsc.2026.106102","url":null,"abstract":"<div><div>Reversible S-palmitoylation, initiated by the Zinc finger aspartate-histidine-histidine-cysteine domain (ZDHHC) proteins and erased by the de-palmitoylases, plays an important role in the antiviral immune pathway. Our previous screening identified ZDHHC7 as a potent restriction factor for PRRSV. However, the mechanisms underlying ZDHHC7-mediated suppression of PRRSV replication remains unclear. Here, we showed that PRRSV RNA replication is impaired by ZDHHC7 in an acyltransferase-dependent manner. This antiviral effect was achieved by S-palmitoylation of Nsp10 (Nonstructural protein 10), which weakened the binding between viral RNA and Nsp10. By cleaving porcine ZDHHC7 at E226, PRRSV Nsp4 impaired the S-palmitoylation of Nsp10, STING, and MAVS. Additionally, the cleaved fragments did not inhibit PRRSV multiplication, whereas the cleavage-resistant mutant ZDHHC7-E226A exhibited enhanced antiviral activity. Finally, we demonstrated that PRRSV Nsp2 and GP5 (glycoprotein 5) induce the transcription of APT1 (Acyl-protein thioesterase 1) through the transcription factor NFATC3 to reverse the S-palmitoylation level of Nsp10. Collectively, this work uncovers the antagonistic strategy employed by PRRSV to dampen the antiviral function of ZDHHC7, which deepens our understanding of the immune evasion mechanisms during PRRSV infection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106102"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From gut health to nutrient digestion and metabolism: An integrative biomarker analysis in nursery pigs","authors":"Marina P. Walter ,&nbsp;Gabriela M. Galli ,&nbsp;Marcos Kipper ,&nbsp;Karine L. Takeuti ,&nbsp;Ines Andretta","doi":"10.1016/j.rvsc.2026.106090","DOIUrl":"10.1016/j.rvsc.2026.106090","url":null,"abstract":"<div><div>This study aimed to explore the relationship between intestinal health biomarkers and nutrient digestion and metabolism in nursery pigs. Thirty-two weaned pigs were individually housed and fed a single experimental diet for 22 days. Feces and urine were collected on days 15 to 21 post-weaning to assess energy and protein digestibility and metabolizability. Blood samples were collected on day 22 to assess intestinal permeability, IgG, and IgA. Fecal samples were collected on the same day to quantify calprotectin, neopterin, lactoferrin, calcium-binding proteins, and fatty acid-binding protein (FABP). All pigs were euthanized on day 22 for intestinal tissue collection to determine villus height, villus width, crypt depth, and villus height-to-crypt depth ratio, thiobarbituric acid reactive substances (TBARS), glutathione S-transferase, protein thiols, resistance to rupture, zonula occludens-1, and occludin. Principal component analysis (PCA), correlation, and classification and regression tree (CART) methods were applied. Fecal calcium-binding proteins and FABP levels were identified in the tree-based models (CART) as key predictors of dry matter digestion. On the other hand, fecal calprotectin and FABP levels were the primary determinants of protein and energy digestibility and metabolizability. Fecal calprotectin levels between 79.4 and 79.6 ng/mL served as the cutoff to distinguish nursery pigs with high and low digestibility in two different CART models. Similarly, the cutoff for FABP levels ranged from 70.5 to 87.4 ng/mL, indicating its role in differentiating groups with high and low efficiency. Our results support the potential of fecal biomarkers as non-invasive indicators of digestibility and metabolism in weaned pigs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106090"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subacute ivermectin–induced affective dysregulation: Evidence for hippocampal microglial and astrocytic activation","authors":"Mustafa Eser ,&nbsp;Cevşen Yazıcı ,&nbsp;Ümmühan Kandemir ,&nbsp;Umut İrfan Üçel ,&nbsp;Mehmet Fatih Bozkurt ,&nbsp;Ümide Demir Özkay ,&nbsp;Özgür Devrim Can","doi":"10.1016/j.rvsc.2026.106105","DOIUrl":"10.1016/j.rvsc.2026.106105","url":null,"abstract":"<div><div>Ivermectin (IVR) is a semi-synthetic macrocyclic lactone widely used against nematodes. Under physiological conditions, its central nervous system (CNS) penetration is limited by P-glycoprotein–mediated efflux at the blood–brain barrier (BBB). Nevertheless, supratherapeutic dosing, BBB-compromising pathologies, concomitant P-glycoprotein inhibitors, or MDR1 variants can enhance CNS entry and precipitate neurotoxicity. To evaluate behavioural and neuroinflammatory sequelae of subacute exposure, rats received IVR (1, 5, or 10 mg/kg; i.p.) once daily for 21 days. Animals were assessed for motor function (activity cage, Rota-rod), affective behaviour (elevated plus maze, modified forced swim test), and cognition (Morris water maze). Assay validation employed diazepam (1 and 3 mg/kg; i.p.), desipramine (30 mg/kg; i.p.), and piracetam (200 mg/kg; i.p.), administered according to their respective protocols. IVR did not alter motor performance or spatial learning and memory across doses. However, 5 and 10 mg/kg reduced the percentage of open-arm entries and increased immobility, consistent with anxiety- and depression-like phenotypes, respectively. At 10 mg/kg, hippocampal immunohistochemistry revealed increased Iba1 and GFAP immunoreactivity within CA1, indicating microglial and astrocytic activation. Quantitative counts demonstrated no significant change in CA1 neuronal number across treatment groups, implying that the observed glial activation occurred in the absence of detectable neuronal loss. Collectively, our data indicate that subacute, high-dose IVR provokes affective behavioural alterations coupled to hippocampal neuroinflammation, while sparing gross motor function and measurable cognitive performance under the present conditions. These findings underscore caution when administering IVR at elevated doses or in settings that compromise BBB integrity and warrant further mechanistic and time-resolved investigation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106105"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Registration, badges, and belonging: Reflections on gender inclusion in the veterinary profession","authors":"Emilian Malek ,&nbsp;Erika Abbondati ,&nbsp;Daniela Denk ,&nbsp;Sarah Kirschner-Kitz ,&nbsp;Maria Jose Navarrete Talloni ,&nbsp;Roberta Rasotto ,&nbsp;Chiara Palmieri","doi":"10.1016/j.rvsc.2026.106106","DOIUrl":"10.1016/j.rvsc.2026.106106","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106106"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146228578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geospatial surveillance of the rabies virus in cattle in Brazil","authors":"Valdir Vieira da Silva ,&nbsp;Denny Parente de Sá Barreto Maia Leite ,&nbsp;Pollyanne Raysa Fernandes de Oliveira ,&nbsp;Lucilene Martins Trindade Gonçalves ,&nbsp;Guilherme Valeriano Silva ,&nbsp;Ana Clara Neves dos Santos ,&nbsp;Raissa Santana Renovato ,&nbsp;Yasmim Victória Santos Pereira ,&nbsp;Rafaela Silva Santos ,&nbsp;Beatriz Maria de Almeida Braz ,&nbsp;Jonatas Campos de Almeida ,&nbsp;Rinaldo Aparecido Mota","doi":"10.1016/j.rvsc.2026.106103","DOIUrl":"10.1016/j.rvsc.2026.106103","url":null,"abstract":"<div><div>In Brazil, bovine rabies causes significant economic losses and poses a risk to both human and veterinary public health. Its persistence is associated with agricultural expansion, environmental factors, and the presence of reservoirs that favor viral circulation. In this context, spatiotemporal analyses are essential to identify high-risk areas and guide effective surveillance strategies. This study aimed to analyze the spatiotemporal, and temporal trends of bovine rabies in Brazil between 2015 and 2024. An ecological study was conducted using data from the National Animal Health Information System (SIZ), applying spatiotemporal, and temporal trend analyses. A total of 7458 cases were reported during the study period, with higher concentrations in the Southeast (28.26%), South (27.76%), and Central-West (21.99%) regions. The highest incidence risks were observed in the South (8.15/100,000), Southeast (5.69/100,000), and Northeast (2.73/100,000). At the state level, Amapá (81.04/100,000), Paraná (8.51/100,000), Pernambuco (8.41/100,000), Amazonas (8.24/100,000), and São Paulo (7.83/100,000) stood out. <em>Desmodus rotundus</em> plays a central role in the maintenance and transmission of rabies virus to herbivores in Brazil, with case density and records of this species overlapping mainly in Minas Gerais, São Paulo, and Paraná, particularly in areas of pasture, temporary crops, and environmental mosaics. Retrospective analysis identified eight high-risk spatiotemporal clusters, with the primary cluster located in the South and Southeast (2015–2019), showing a relative risk (RR) of 2.86. Prospective analysis revealed five recent clusters, with the primary one located in Paraná and Santa Catarina (2022–2024; RR = 3.88). Joinpoint regression demonstrated an overall decreasing trend in incidence risk (APC: −8.6%). This is the first nationwide study integrating spatiotemporal, and temporal analyses of bovine rabies, highlighting its widespread occurrence across Brazil and identifying strategic areas for strengthening control measures.</div></div><div><h3>Keywords</h3><div>Bovine rabies</div><div>Spatiotemporal analysis</div><div>Epidemiology</div><div>Risk clusters</div><div>Brazil</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106103"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}