Research in veterinary science最新文献

{"title":"Rehabilitating wild animal welfare: A focus on veterinary rescue and rehabilitation interventions","authors":"Michaël Beaulieu","doi":"10.1016/j.rvsc.2025.105582","DOIUrl":"10.1016/j.rvsc.2025.105582","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105582"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary study of neutrophils and neutrophil extracellular traps (NETs) in canine mammary tumors","authors":"Gulsum Karaman ,&nbsp;Volkan Ipek","doi":"10.1016/j.rvsc.2025.105573","DOIUrl":"10.1016/j.rvsc.2025.105573","url":null,"abstract":"<div><div>Neutrophils play a complex role in cancer biology, can contributing to tumor progression and immune defense. Neutrophil extracellular traps (NETs) have emerged as key modulators within the tumor microenvironment. Herein, the association between molecular classification, histological grade, necrosis, tumor-infiltrating neutrophils, and NETs was assessed in 19 canine mammary malignant tumors. Immunohistochemistry using citrullinated histone-3 (cith3) and myeloperoxidase (MPO) antibodies were used to detect NETs. A fading and re-staining method was applied on the same sections. NETs were scored based on the presence of cith3 positive areas and compared with tumor grade. The neutrophil score numerically increased as the tumor grade increased. The NET score was slightly higher in grade I carcinomas compared to carcinomas with other grades. On contrary, the necrosis score was also higher in grade II and III tumors than grade I tumors. A low but non-significant negative correlation existed between tumor grade and NET score (<em>r</em> = −0.219). No statistically significant associations between the tumor markers (ER, PR, HER2) and molecular subtypes with tumor grade, NET score, neutrophil count, and necrosis. In this study, the presence of NETs in canine malignant mammary tumor of different histological subtypes and grades was reported. Preliminary evidence was gathered that NETs are negatively correlated with tumor grade, suggesting their potential role in prognostication.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105573"},"PeriodicalIF":2.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of 3D printing in veterinarian science","authors":"Hinpetch Daungsupawong ,&nbsp;Viroj Wiwanitkit","doi":"10.1016/j.rvsc.2025.105571","DOIUrl":"10.1016/j.rvsc.2025.105571","url":null,"abstract":"<div><div>This article explores the transformative impact of 3D printing technology in veterinary medicine. It focuses on applications in various areas such as prosthetics and surgical planning. 3D printing allows for the creation of customized solutions for each animal, thus improving treatment outcomes and reducing surgical risks. Case studies demonstrate the successful adoption of this technology. It highlights innovations that improve patient care and improve veterinary practice. The discussion highlights the future potential of 3D printing in the advancement of veterinary medicine. It also considers ethical and practical challenges.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105571"},"PeriodicalIF":2.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in thickness of dermal layers in cranio-caudal anatomic locations in porcine skin wounds: Implications for wound healing studies","authors":"Guillermo Villa-Martinez ,&nbsp;Hsin-ya Yang ,&nbsp;Anthony Gallegos ,&nbsp;Sriansh Pasumarthi ,&nbsp;Elham Aslankoohi ,&nbsp;Marco Rolandi ,&nbsp;William Ferrier ,&nbsp;Roslyn Rivkah Isseroff","doi":"10.1016/j.rvsc.2025.105572","DOIUrl":"10.1016/j.rvsc.2025.105572","url":null,"abstract":"<div><div>The use of the domesticated swine is important for advancing our understanding of healing in human skin due to the architectural similarities in their skin. In swine wound studies, skin thickness may play a role in healing due to the anatomical variation in the thickness of the different skin compartments, such as the dermis and subcutaneous fat, as well as the differences in anatomical location of the wounds. Here, the thickness of different skin compartments were measured in excisional wounds created in different anatomical locations along the cranio-caudal axis. Full-thickness, 2 cm diameter circular excisions were created on the dorsal paravertebral area of pigs. Thickness of the dermal and adipose layers of excised tissues at day 3 post-excision were measured in fixed H&amp;E sections. Wound re-epithelialization was measured on excised day 10 wounds. A significant difference in skin thickness between the cranial wounds compared to the caudal wounds is noted in skin excised down to the landmark of the subcutaneous fascial plane. There were also significant differences in the thickness of the dermal and fat layers. Skin thickness, and the variations of the dermal and fat compartments, and locations of wounds should be taken into consideration in porcine wound studies as they may contribute to alterations in healing in the different locations.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105572"},"PeriodicalIF":2.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an RFLP-PCR assay for discrimination of three prevalent Old world Leishmania species in canine and human samples","authors":"Fatemeh Fazeli ,&nbsp;Ehsan Rakhshandehroo ,&nbsp;Hassan Sharifiyazdi ,&nbsp;Tina Yaghoobpour","doi":"10.1016/j.rvsc.2025.105570","DOIUrl":"10.1016/j.rvsc.2025.105570","url":null,"abstract":"<div><div>Dogs in endemic regions are susceptible to leishmaniasis, a common vector-borne illness. Serological techniques and direct parasitological inquiry have been employed for diagnosis. However, molecular techniques are more sensitive and specific for identifying <em>Leishmania</em> infection in both clinically healthy and diseased dogs. This research aims to develop an approach for molecular identification of the parasite using restriction fragment length polymorphism (RFLP) analysis. Samples of skin and lymph nodes were taken from suspected dogs and humans and smears were prepared. DNA was extracted, and PCR reactions targeting the ribosomal internal transcribed spacer 1 (ITS1) region were used to identify the infection. Also, the PCR products were sequenced to provide control specimens. Before conducting an RFLP analysis, a bioinformatic study was performed on related sequences from the GenBank, as well as some previous data, to verify the results of <em>RsaI</em> and <em>ApoI</em> enzymatic digestion. The ITS1 PCR products were then subjected to digestion by these two enzymes, and the resulted pattern on the agarose gel were compared to those produced by the enzyme <em>Hae</em>III, commonly used in other studies. Using the <em>Rsa</em>I enzyme, fragments of approximately 95 and 146 bp were obtained for L. <em>infantum</em>, while <em>L. tropica</em> and <em>L. major</em> remained uncut. <em>Apo</em>I produced fragments of 101 and 140 bp for L. <em>infantum</em> and 108 and 157 bp for L. <em>major</em>. Based on these results, RFLP analysis with <em>Rsa</em>I and <em>Apo</em>I proved to be a reliable tool for detecting different <em>Leishmania</em> species using a defined algorithm.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105570"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association analysis reveals genetic loci and candidate genes for white diarrhea in Jingyuan chickens","authors":"Lijuan Yang ,&nbsp;Siyu Chen ,&nbsp;Wei Zhao ,&nbsp;Guojun Zhang ,&nbsp;Hu Zhang ,&nbsp;Tong Zhang ,&nbsp;Lin Xue ,&nbsp;Jinli Tian ,&nbsp;Yaling Gu ,&nbsp;Lanlan Li ,&nbsp;Hu Wang ,&nbsp;Juan Zhang","doi":"10.1016/j.rvsc.2025.105568","DOIUrl":"10.1016/j.rvsc.2025.105568","url":null,"abstract":"<div><div>In order to investigate the basic genetic structure of dysentery in Jingyuan chickens and to explore the genetic markers associated with dysentery resistance in chickens, the present study was based on the genome-wide association analysis (GWAS) technique to investigate the candidate SNPs and genes associated with dysentery resistance in Jingyuan chickens, and a total of 12 SNPs were associated with dysentery resistance in Jingyuan chickens. In addition, some important candidate genes inciuding frizzled class receptor 4 (<em>FZD4</em>), DDB1 and CUL4 associated factor 13(<em>DCAF13</em>), regulating synaptic membrane exocytosis 2 (<em>RIMS2</em>), transmembrane protein 8C (<em>TMEM8C</em>), and RIC1 homolog (<em>RIC1</em>) were identified by selection signal analysis, gene annotation, and enrichment analysis. These results can be used as potential molecular selection markers for chicken dysentery resistance in Jingyuan chickens in order to improve the breeding of disease resistance in Jingyuan chickens.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105568"},"PeriodicalIF":2.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How many subjects are enough in a veterinary trial?—Literature review and insights from industrial statisticians","authors":"Zikun Wang ,&nbsp;Qi Cao ,&nbsp;Qingzhi Liu ,&nbsp;Divine Dufe ,&nbsp;Pieter Wouters ,&nbsp;Lijuan Deng ,&nbsp;Annpey Pong","doi":"10.1016/j.rvsc.2025.105569","DOIUrl":"10.1016/j.rvsc.2025.105569","url":null,"abstract":"<div><div>To approve veterinary medicines, regulatory agencies require substantial evidence of safety and effectiveness from well-designed trials. A critical consideration in planning a veterinary trial is to determine the requisite number of subjects needed to achieve the trial objective(s). Sample sizes for preclinical studies usually follow specific guidelines, whereas clinical studies should follow more rigorous requirements to determine the optimal sample size for detecting significant and meaningful treatment effects. This paper presents a descriptive literature review aiming to explore current reported practices in sample size calculation for published veterinary clinical trials. The review included articles published in 12 top-ranking veterinary health journals from January 1, 2013, to June 4, 2023, sourced from PubMed and Medline regarding animal trials. Initially, 294 articles were identified, of which 98 (33 %) focused on veterinary clinical studies. Among these 98 papers, only 24 (24 %) provided detailed methods on sample size calculations, with individual animals as experimental units across at least two arms. The predominant animal species studied in these trials were canines (39 %), cattle (32 %), and swine (21 %), with most studies aiming for a statistical power of at least 80 %. Unlike human clinical trials, the paper found that statistically rigorous sample size calculations were less commonly reported in animal clinical trials. Our paper provides recommendations for veterinary clinical trial practitioners and offers insights into how sample size determination can be properly conducted and reported. Furthermore, this article extends to discuss practical issues in sample size determination for preclinical studies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105569"},"PeriodicalIF":2.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic potential of mesenchymal stromal cell secretome in treating spontaneous chronic corneal epithelial defects in dogs","authors":"Alejandro Casado-Santos ,&nbsp;Elsa González-Cubero ,&nbsp;Mª. Belén García-Rodríguez ,&nbsp;Álvaro Carrera-Serna ,&nbsp;Mª Luisa González-Fernández ,&nbsp;Vega Villar-Suárez","doi":"10.1016/j.rvsc.2025.105559","DOIUrl":"10.1016/j.rvsc.2025.105559","url":null,"abstract":"<div><div>Corneal ulcers in dogs pose a significant challenge in veterinary ophthalmology, often leading to prolonged visual impairment and discomfort. This study aimed to assess the efficacy of adipose tissue-derived mesenchymal stromal cell (ASCs) secretome as a treatment for complicated corneal ulcers in dogs. Ten dogs with spontaneous chronic corneal epithelial defects, were treated with topical application of ASC secretome eye drops. Our results showed that secretome therapy facilitated complete healing of all corneal ulcers within 4 weeks, with an average healing time of 1.2 weeks. Notably, secretome treatment was effective even in cases that had previously failed to respond to conventional therapies. Clinical signs such as blepharospasm, conjunctival hyperemia, and photophobia were alleviated promptly following secretome administration. Secretome therapy was well-tolerated, with no adverse reactions reported, further supporting its safety profile. The findings suggest that ASC secretome represents a promising cell-free and minimally invasive therapeutic approach for the treatment of complicated corneal ulcers in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105559"},"PeriodicalIF":2.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine fecal biomarkers of intestinal inflammatory process: Calprotectin and lactoferrin, a comparative study","authors":"Yolanda Saco ,&nbsp;Núria Crusellas-Villorbina ,&nbsp;Raquel Peña ,&nbsp;Raquel Pato ,&nbsp;Sonia Marti ,&nbsp;Lucía Pisoni ,&nbsp;Maria Devant ,&nbsp;Anna Pelegrí-Pineda ,&nbsp;Anna Bassols","doi":"10.1016/j.rvsc.2024.105500","DOIUrl":"10.1016/j.rvsc.2024.105500","url":null,"abstract":"<div><div>Fecal lactoferrin and fecal calprotectin have been proposed as biomarkers of intestinal inflammation in several animal species. The main objectives of this work were to validate an analytical procedure for the measurement of lactoferrin in calf feces, to study the correlation between lactoferrin and calprotectin concentrations, and to evaluate the influence of fecal water content in the determination of these proteins. This knowledge is essential for effectively using these biomarkers in young calves exposed to inflammatory gastrointestinal diseases.Seventy-eight male Holstein dairy calves between two and three weeks of age were included in the study. Lactoferrin was determined with a bovine milk lactoferrin ELISA kit and calprotectin was measured using a human immunoturbidimetric method previously validated in bovine feces. Analytical validation of the lactoferrin assay achieved good results, with intra and inter assay CV &lt; 10 %, recovery rates between 80 and 120 %, and optimal linearity under dilution. A robust correlation was observed between fecal calprotectin concentrations in dry and wet feces (<em>r</em> = 0.903), while a moderate correlation was observed for fecal lactoferrin concentrations (<em>r</em> = 0.648). Correlation between both biomarkers was moderate in fresh feces (<em>r</em> = 0.514) as well as in dry feces (<em>r</em> = 0.561). In conclusion, the lactoferrin ELISA kit is valid for its use with calf fecal samples, both biomarkers showed a moderate correlation between them, and fecal lactoferrin concentration is more influenced by feces moisture than fecal calprotectin concentration.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105500"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of chromatin accessibility in porcine oocytes from follicles of different sizes","authors":"Yazhuo Cheng ,&nbsp;Jingyu Li ,&nbsp;Jiyong Shang ,&nbsp;Xilong Jia ,&nbsp;Yeling Bi ,&nbsp;Jiaxu Gu ,&nbsp;Nan Jiang ,&nbsp;Yanjun Huan ,&nbsp;Youwei Li ,&nbsp;Mingju Sun","doi":"10.1016/j.rvsc.2025.105565","DOIUrl":"10.1016/j.rvsc.2025.105565","url":null,"abstract":"<div><div>Chromatin accessibility and transcription levels during oocyte growth are important for oocyte maturation and subsequent development. However, chromatin accessibility changes in porcine oocytes during growth are unclear. The present study demonstrated that porcine oocytes derived from large follicles (LFO) exhibited higher developmental capacity than those derived from small follicles (SFO). Assay for transposase-accessible chromatin using sequencing (ATAC-seq) analysis identified 1117 and 1694 uniquely accessible chromatin peaks in LFO and SFO, respectively. Motif analysis of differential peaks revealed the top 10 significantly enriched transcription factor (TF)-binding motifs in LFO versus SFO, with only one increased peak (<em>Spi1</em> binding site) and nine decreased peaks (<em>NFYA</em>, <em>ATOH1</em>, <em>ZNF549</em>, <em>Foxn1</em>, <em>HAND2</em>, <em>THRB</em>, <em>NHLH2</em>, <em>FoxP1</em>, and <em>FoxP2</em> binding sites). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified key processes in the regulation of oocyte growth and maturation. Integration of ATAC-seq and RNA sequencing data revealed the top 10 hub genes involved in chromatin remodeling (<em>MYSM1</em> and <em>EZH2</em>), histone modification (<em>MYSM1</em>, <em>RNF2</em>, <em>USP1</em>, <em>EZH2</em>, and <em>MIER1</em>), and transcription regulation (<em>MYSM1</em>, <em>ASXL3</em>, and <em>MIER1</em>), as well as those involved in metabolic processes and signal transduction (<em>DOCK7</em>, <em>FGGY</em>, <em>DTL</em>, and <em>DNAJC6</em>). All these genes exhibited increased expression levels in LFO versus SFO. In conclusion, the study demonstrated the dynamic nature of chromatin accessibility during porcine oocyte growth and revealed the TFs and genes closely associated with oocyte growth and maturation. These findings provide new insight into porcine oocyte growth and offer a potential strategy to enhance the in vitro developmental ability of SFO.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105565"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}