Research in veterinary science最新文献

{"title":"Influence of dietary fat source and lysolecithin on growing pigs.","authors":"Gergana Yordanova, Radka Dimitrova Nedeva, Maryana Petrova, Katya Eneva, Sarah Icely, Stephen Charles Mansbridge, Vasil Radoslavov Pirgozliev","doi":"10.1016/j.rvsc.2026.106455","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106455","url":null,"abstract":"<p><p>Insect derived products are an emerging field but need careful consideration in animal nutrition due to their saturated nature. This report describes a digestibility experiment performed on 40 male and female growing Danube White pigs of initial age 93 days and body weight of 54.50 kg (SDEV ±6.95). Dietary treatments were prepared according to a 2 × 2 factorial design, which included two lysolecithin (LL) levels (0 or 1 g/kg diet) and two fat sources: unrefined sunflower oil (USFO) or fat from Black Soldier Fly larvae (BSLF), both at 40 g/kg diet. Fat source by LL interactions (P < 0.05) were observed for digestible energy (DE), dry matter digestibility (DMD) and fibre digestion (FibD), as the diet containing USFO without LL showed the lowest DE and respective coefficients. Fat digestibility coefficient was not affected (P > 0.05) by fat type or LL supplementation. Lysolecithin increased (P < 0.05) dietary nitrogen digestibility (ND) and there was a tendency (P = 0.056) of LL by fat type interaction, following the same pattern as those of DE, DMD and FibD. There were no significant effects (P > 0.05) of dietary LL supplementation or fat source on growth performance of pigs. The results showed that the examined BSLF is a good source of DE and can be incorporated in pig diets as an alternative energy source to vegetable fats. However, further research is needed to study the interaction between insect fats and LL.</p>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"214 ","pages":"106455"},"PeriodicalIF":2.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149380559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrageenan-mediated modulation of inflammatory responses promotes type 17-associated reparative mucosal immunity during Eimeria tenella infection in chickens.","authors":"Rin Huu Dong, Makoto Matsubayashi, Toshimitsu Hatabu","doi":"10.1016/j.rvsc.2026.106454","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106454","url":null,"abstract":"<p><p>Protective immunity against Eimeria tenella infection has traditionally been attributed to strong inflammatory and cell-mediated immune responses. However, excessive inflammation can contribute to intestinal pathology and disease severity. This study investigated whether carrageenan-mediated modulation of inflammatory and antigen-presenting cell-associated responses influences the balance between inflammatory and tissue-protective immunity during primary E. tenella infection in chickens. Carrageenan was administered intraperitoneally at 12, 13, and 16 days of age, and its effects on antigen-presenting cell-associated markers and local immune responses were evaluated. Reduced expression of MHC-II and CD80 confirmed the alteration of antigen-presenting cell-associated markers in carrageenan-treated infected chicks (CAR). Compared with infected controls, CAR group exhibited significantly lower lesion scores and parasite burdens at 3 and 5 days post-infection (dpi). Expression of inflammatory mediators, including interleukin (IL)-1β, interferon-γ, CD8, NLRP3, and caspase-1, was markedly lower in the CAR group at 5 dpi. Conversely, IL-17 A and IL-22 expression was significantly elevated in the CAR group, whereas Arg1 expression showed an increasing trend. Expression of the pro-apoptotic marker Bax was reduced by carrageenan treatment, accompanied by attenuated intestinal tissue damage. These findings suggest that carrageenan-associated modulation of inflammatory and antigen-presenting cell-associated responses was accompanied by a shift in the local immune environment toward a type 17-associated reparative mucosal response. This response was associated with a reduced parasite burden, decreased intestinal pathology, and improved mucosal integrity. Our results suggest that the outcome of primary E. tenella infection is determined by both inflammatory parasite-control mechanisms and tissue-protective immune pathways that promote mucosal repair and resilience.</p>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"214 ","pages":"106454"},"PeriodicalIF":2.1,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149380558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Cadaveric study of the ultrasound-guided inter-transversospinalis plane block in dogs for the cervical epaxial musculature region\" [Res. Vet. Sci. 180 (2024) 105427].","authors":"Manuel Enrique Herrera-Linares, Rocío Fernández-Parra, Carla Zamora-Perarnau, Nuria Fernández-Salesa, Dylan Yaffy, Sandra Sanchis-Mora","doi":"10.1016/j.rvsc.2026.106435","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106435","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":" ","pages":"106435"},"PeriodicalIF":2.1,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Network pharmacology-based identification and validation of novel vaccine candidates against brucellosis\" [Res. Vet. Sci., 211 (2026), 106359].","authors":"Zhiheng Dong, Sha Li, Jiarong Guo, Lidao Bao","doi":"10.1016/j.rvsc.2026.106426","DOIUrl":"https://doi.org/10.1016/j.rvsc.2026.106426","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":" ","pages":"106426"},"PeriodicalIF":2.1,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical disc disease in whippets: Results of an online survey involving Italian owners and breeders","authors":"Diletta Dell'Apa ,&nbsp;Ezio Bianchi ,&nbsp;Luigi Auletta ,&nbsp;Mariaelena de Chiara ,&nbsp;Fabiana Micieli ,&nbsp;Francesco Ferrari ,&nbsp;Barbara Lamagna ,&nbsp;Maria Pia Pasolini","doi":"10.1016/j.rvsc.2026.106068","DOIUrl":"10.1016/j.rvsc.2026.106068","url":null,"abstract":"<div><div>Whippet is considered quite a healthy breed. Nonetheless, the perception of some veterinary neurologists is that the occurrence of neurological diseases, especially cervical disc disease (CDD), is frequent in this breed. This study aimed to get information about the prevalence of the most common disorders and conditions affecting the Italian Whippet population, particularly investigating the prevalence of CDD and the perception of the problem by owners and breeders. Owners and breeders included in the Italian Sighthound Club register were invited to answer anonymously an online questionnaire. Descriptive statistics were used for breeder/owner and dog characteristics, and statistical association with the development of CDD were explored. Over 280 Whippets, the overall prevalence of CDD was 7.2% and 29% in Whippets older than 9 years. Whippets older than 9 years were overrepresented in the CDD group (<em>P</em> &lt; 0.0001; O.R. 0.07; 95% CI: 0.02–0.22) compared with the CDD group. These findings confirm the hypothesis that CDD may be a challenging and more prevalent than expected disorder in this breed and health screening programs could be recommended to breeders and owners to assess cervical spine conformation, especially before sporting activity or breeding.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106068"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA-mRNA integrative profiling in canine tubulopapillary mammary carcinoma","authors":"Nayra Cristina Herreira do Valle ,&nbsp;Matheus Fujimura Soares ,&nbsp;Natália Francisco Scaramele ,&nbsp;Amanda de Oliveira Furlan ,&nbsp;Maria Fernanda da Silva Lopes ,&nbsp;Juliana de Souza Felix ,&nbsp;Fernando Vissani Fernandes ,&nbsp;Vinicius Aquiles Gomes Zamboni ,&nbsp;Igor Luiz Salardani Senhorello ,&nbsp;Alexandre Lima de Andrade ,&nbsp;Flavia Lombardi Lopes","doi":"10.1016/j.rvsc.2026.106107","DOIUrl":"10.1016/j.rvsc.2026.106107","url":null,"abstract":"<div><div>Canine mammary tumors (CMTs) are the most common neoplasia in intact female dogs. There are different histological subtypes, which are related to tumor behavior and prognosis. Among them, we highlight the tubulopapillary subtype, one of the most prevalent subtypes in female dogs. Although not the most aggressive subtype overall, it is associated with significantly higher lethality compared to other common subtypes, suggesting an underlying dysregulation of gene expression. However, global expression of RNAs in tubulopapillary CMTs is not available in the literature. Here, we analyzed the differential expression of mRNAs and miRNAs of tubulopapillary CMTs (<em>n</em> = 4) and compared them with normal mammary tissues (n = 4) using microarray. We found 842 downregulated and 385 upregulated mRNAs in the CMTs, which corresponded to the enrichment of 10 biological pathways. Also, we found 30 upregulated miRNAs and one downregulated (cfa-miR-345) in CMTs. Among the differentially expressed mRNAs, we identified 360 mRNAs that were targets of the differentially expressed miRNAs, which enriched 23 biological pathways, with the “Endocytosis” pathway being of particular significance. These findings highlight the complex interactions between mRNAs and miRNAs in tubulopapillary CMTs, thereby improving the understanding of the pathophysiology of this CMT subtype and providing targets for functional validation of biomarkers and/or therapeutics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106107"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S-palmitoylation of the viral Nsp10 by ZDHHC7 restricts PRRSV replication but is counteracted by Nsp2, Nsp4 and GP5","authors":"Huiyuan Jing ,&nbsp;Zhifeng Peng ,&nbsp;Pandeng Zhao ,&nbsp;Erzhen Duan ,&nbsp;Jie Liu ,&nbsp;Xuyong Zhao ,&nbsp;Huawei Li","doi":"10.1016/j.rvsc.2026.106102","DOIUrl":"10.1016/j.rvsc.2026.106102","url":null,"abstract":"<div><div>Reversible S-palmitoylation, initiated by the Zinc finger aspartate-histidine-histidine-cysteine domain (ZDHHC) proteins and erased by the de-palmitoylases, plays an important role in the antiviral immune pathway. Our previous screening identified ZDHHC7 as a potent restriction factor for PRRSV. However, the mechanisms underlying ZDHHC7-mediated suppression of PRRSV replication remains unclear. Here, we showed that PRRSV RNA replication is impaired by ZDHHC7 in an acyltransferase-dependent manner. This antiviral effect was achieved by S-palmitoylation of Nsp10 (Nonstructural protein 10), which weakened the binding between viral RNA and Nsp10. By cleaving porcine ZDHHC7 at E226, PRRSV Nsp4 impaired the S-palmitoylation of Nsp10, STING, and MAVS. Additionally, the cleaved fragments did not inhibit PRRSV multiplication, whereas the cleavage-resistant mutant ZDHHC7-E226A exhibited enhanced antiviral activity. Finally, we demonstrated that PRRSV Nsp2 and GP5 (glycoprotein 5) induce the transcription of APT1 (Acyl-protein thioesterase 1) through the transcription factor NFATC3 to reverse the S-palmitoylation level of Nsp10. Collectively, this work uncovers the antagonistic strategy employed by PRRSV to dampen the antiviral function of ZDHHC7, which deepens our understanding of the immune evasion mechanisms during PRRSV infection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106102"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From gut health to nutrient digestion and metabolism: An integrative biomarker analysis in nursery pigs","authors":"Marina P. Walter ,&nbsp;Gabriela M. Galli ,&nbsp;Marcos Kipper ,&nbsp;Karine L. Takeuti ,&nbsp;Ines Andretta","doi":"10.1016/j.rvsc.2026.106090","DOIUrl":"10.1016/j.rvsc.2026.106090","url":null,"abstract":"<div><div>This study aimed to explore the relationship between intestinal health biomarkers and nutrient digestion and metabolism in nursery pigs. Thirty-two weaned pigs were individually housed and fed a single experimental diet for 22 days. Feces and urine were collected on days 15 to 21 post-weaning to assess energy and protein digestibility and metabolizability. Blood samples were collected on day 22 to assess intestinal permeability, IgG, and IgA. Fecal samples were collected on the same day to quantify calprotectin, neopterin, lactoferrin, calcium-binding proteins, and fatty acid-binding protein (FABP). All pigs were euthanized on day 22 for intestinal tissue collection to determine villus height, villus width, crypt depth, and villus height-to-crypt depth ratio, thiobarbituric acid reactive substances (TBARS), glutathione S-transferase, protein thiols, resistance to rupture, zonula occludens-1, and occludin. Principal component analysis (PCA), correlation, and classification and regression tree (CART) methods were applied. Fecal calcium-binding proteins and FABP levels were identified in the tree-based models (CART) as key predictors of dry matter digestion. On the other hand, fecal calprotectin and FABP levels were the primary determinants of protein and energy digestibility and metabolizability. Fecal calprotectin levels between 79.4 and 79.6 ng/mL served as the cutoff to distinguish nursery pigs with high and low digestibility in two different CART models. Similarly, the cutoff for FABP levels ranged from 70.5 to 87.4 ng/mL, indicating its role in differentiating groups with high and low efficiency. Our results support the potential of fecal biomarkers as non-invasive indicators of digestibility and metabolism in weaned pigs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106090"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subacute ivermectin–induced affective dysregulation: Evidence for hippocampal microglial and astrocytic activation","authors":"Mustafa Eser ,&nbsp;Cevşen Yazıcı ,&nbsp;Ümmühan Kandemir ,&nbsp;Umut İrfan Üçel ,&nbsp;Mehmet Fatih Bozkurt ,&nbsp;Ümide Demir Özkay ,&nbsp;Özgür Devrim Can","doi":"10.1016/j.rvsc.2026.106105","DOIUrl":"10.1016/j.rvsc.2026.106105","url":null,"abstract":"<div><div>Ivermectin (IVR) is a semi-synthetic macrocyclic lactone widely used against nematodes. Under physiological conditions, its central nervous system (CNS) penetration is limited by P-glycoprotein–mediated efflux at the blood–brain barrier (BBB). Nevertheless, supratherapeutic dosing, BBB-compromising pathologies, concomitant P-glycoprotein inhibitors, or MDR1 variants can enhance CNS entry and precipitate neurotoxicity. To evaluate behavioural and neuroinflammatory sequelae of subacute exposure, rats received IVR (1, 5, or 10 mg/kg; i.p.) once daily for 21 days. Animals were assessed for motor function (activity cage, Rota-rod), affective behaviour (elevated plus maze, modified forced swim test), and cognition (Morris water maze). Assay validation employed diazepam (1 and 3 mg/kg; i.p.), desipramine (30 mg/kg; i.p.), and piracetam (200 mg/kg; i.p.), administered according to their respective protocols. IVR did not alter motor performance or spatial learning and memory across doses. However, 5 and 10 mg/kg reduced the percentage of open-arm entries and increased immobility, consistent with anxiety- and depression-like phenotypes, respectively. At 10 mg/kg, hippocampal immunohistochemistry revealed increased Iba1 and GFAP immunoreactivity within CA1, indicating microglial and astrocytic activation. Quantitative counts demonstrated no significant change in CA1 neuronal number across treatment groups, implying that the observed glial activation occurred in the absence of detectable neuronal loss. Collectively, our data indicate that subacute, high-dose IVR provokes affective behavioural alterations coupled to hippocampal neuroinflammation, while sparing gross motor function and measurable cognitive performance under the present conditions. These findings underscore caution when administering IVR at elevated doses or in settings that compromise BBB integrity and warrant further mechanistic and time-resolved investigation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106105"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Registration, badges, and belonging: Reflections on gender inclusion in the veterinary profession","authors":"Emilian Malek ,&nbsp;Erika Abbondati ,&nbsp;Daniela Denk ,&nbsp;Sarah Kirschner-Kitz ,&nbsp;Maria Jose Navarrete Talloni ,&nbsp;Roberta Rasotto ,&nbsp;Chiara Palmieri","doi":"10.1016/j.rvsc.2026.106106","DOIUrl":"10.1016/j.rvsc.2026.106106","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106106"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146228578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}