Research in veterinary science最新文献

{"title":"Isolation of SSEA-3-positive mesenchymal stem cells from equine bone marrow and evaluation of their pluripotency","authors":"Sangchan Lee ,&nbsp;Mya Theingi Kyaw ,&nbsp;Koji Harada ,&nbsp;Ken Takeshi Kusakabe ,&nbsp;Masaya Igase ,&nbsp;Naoki Sasaki","doi":"10.1016/j.rvsc.2025.105736","DOIUrl":"10.1016/j.rvsc.2025.105736","url":null,"abstract":"<div><div>Generally, most mesenchymal stem cells (MSCs) have lower pluripotency and limited differentiation potential than embryonic stem cells (ESCs). However, a small subpopulation of MSCs, called multilineage differentiating stress-enduring (MUSE) cells, exhibit pluripotency. MUSE cells express stage-specific embryonic antigen 3 (SSEA-3), a sphingoglycolipid. Here, we isolated and investigated the pluripotency of SSEA-3-positive MSCs (MUSE cells).</div><div>Six thoroughbred horses were used as test subjects. MSCs were harvested from the bone marrow of the thoracic vertebrae under ultrasound guidance. Harvested MSCs were sorted into SSEA-3-positive and -negative cells using a cell sorter, and their pluripotency was evaluated by immunostaining for the markers NANOG, OCT4, and SOX2.</div><div>The results showed that 2.1 % of equine MSCs were SSEA-3-positive, and these cells were successfully isolated using a cell sorter. This study confirms that SSEA-3-positive cells are present in equine bone marrow-derived MSCs, similar to that in other species.</div><div>Immunostaining revealed SOX2 (88.3 ± 11.6 %) and NANOG expressions (9.0 ± 3.9 %) in the SSEA-3-negative cell group, whereas OCT4 expression was not detected (0.0 ± 0.0 %). Contrastingly, the SSEA-3-positive cell group showed significant NANOG (19.8 ± 3.8 %), OCT4 (9.7 ± 2.6 %), and SOX2 expressions (97.3 ± 4.1 %), with significantly higher values for NANOG and OCT4 compared to the SSEA-3-negative group (<em>p</em> &lt; 0.05).</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105736"},"PeriodicalIF":2.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facial action units as biomarkers of postoperative pain in ovariohysterectomized bitches treated with cannabidiol and meloxicam","authors":"Alejandro Casas-Alvarado ,&nbsp;Julio Martínez-Burnes ,&nbsp;Patricia Mora-Medina ,&nbsp;Ismael Hernández-Avalos ,&nbsp;Agatha Miranda-Cortes ,&nbsp;Adriana Domínguez-Oliva ,&nbsp;Daniel Mota-Rojas","doi":"10.1016/j.rvsc.2025.105734","DOIUrl":"10.1016/j.rvsc.2025.105734","url":null,"abstract":"<div><div>Facial expression in animals is a suggested method to assess the subtle facial changes associated with pain. This study aimed to evaluate postoperative pain and establish its correlation with facial action units (AUs) in female dogs undergoing elective ovariohysterectomy (OVH) treated with cannabidiol alone or in combination with meloxicam. Sixty healthy female dogs of different breeds, were randomly assigned into four groups according to the treatment. The control group (G₀: <em>n</em> = 15) received a saline solution as placebo; the meloxicam group (G_Mlx: n = 15) received meloxicam at a dose of 0.2 mg kg⁻¹ IV, followed by 0.1 mg kg⁻¹ every 24 h, for 48 h during the postoperative period; the cannabidiol group (G_CBD: <em>n</em> = 15) received 2 mg kg⁻¹ PO every 12 h; and the combination group (G_Mlx/CBD: n = 15) received both treatments. The treatments were administered 30 min before starting the surgery. High-resolution videos were recorded for one minute at baseline and multiple time points up to 48 h post-surgery to quantify facial action units: AU101, AU143 + 145, AU12, ear position, facial muscle tension, orbital tightening, and lip tension. Data was analyzed with a mixed linear model. The frequency of AU143 + 145, AU12, ear position, tension of the facial muscles, orbital tightening, and tension of the lips significantly increased at 30 min, 1 h, 2 h, and 3 h post-surgery (<em>p</em> = 0.0001) in the control group. Thus, facial expressions associated with acute pain in dogs were characterized by AUs such as blink (AU143 + 145), lip corner puller (AU12), ear position, head position, tension of the facial muscles, orbital tightening, and tension of the lips, which maintained a positive moderate correlation with UMPS scores, this association indicates that these facial changes are signs of acute pain in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105734"},"PeriodicalIF":2.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of concern: \"Impact of Cryptosporidium parvum infection on glucose transport and metabolism in calves: Physiological and molecular insight\" [Research in Veterinary Science (2024), In press].","authors":"","doi":"10.1016/j.rvsc.2025.105566","DOIUrl":"https://doi.org/10.1016/j.rvsc.2025.105566","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"189 ","pages":"105566"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LiDSCUNet++: A lightweight depth separable convolutional UNet++ for vertebral column segmentation and spondylosis detection","authors":"Krishna K. Agrawal,&nbsp;Gautam Kumar","doi":"10.1016/j.rvsc.2025.105703","DOIUrl":"10.1016/j.rvsc.2025.105703","url":null,"abstract":"<div><div>Accurate computer-aided diagnosis systems rely on precise segmentation of the vertebral column to assist physicians in diagnosing various disorders. However, segmenting spinal disks and bones becomes challenging in the presence of abnormalities and complex anatomical structures. While Deep Convolutional Neural Networks (DCNNs) achieve remarkable results in medical image segmentation, their performance is limited by data insufficiency and the high computational complexity of existing solutions. This paper introduces LiDSCUNet++, a lightweight deep learning framework based on depthwise-separable and pointwise convolutions integrated with UNet++ for vertebral column segmentation. The model segments vertebral anomalies from dog radiographs, and the results are further processed by YOLOv8 for automated detection of Spondylosis Deformans. LiDSCUNet++ delivers comparable segmentation performance while significantly reducing trainable parameters, memory usage, energy consumption, and computational time, making it an efficient and practical solution for medical image analysis.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105703"},"PeriodicalIF":2.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γδ T cells induced by zoledronate under macrophage-depleted conditions reduce disease severity and parasite number in Eimeria tenella-infected chicks","authors":"Quan Viet Le ,&nbsp;Makoto Matsubayashi ,&nbsp;Toshimitsu Hatabu","doi":"10.1016/j.rvsc.2025.105730","DOIUrl":"10.1016/j.rvsc.2025.105730","url":null,"abstract":"<div><div>Disease severity and local immune responses mediated by Zoledronate (ZOL)-induced γδ T cells were evaluated in chicks infected with <em>E. tenella</em> under macrophage-depleted conditions. Three groups of White Leghorns, including a negative control group (CT), carrageenan-treated group (CAR), and both ZOL and CAR-treated group (ZOL/CAR), were infected orally with <em>E. tenella</em> sporulated oocysts (1 × 10⁴ oocysts/chick) at 14 days of age. Fecal oocyst shedding was assessed at 4–15 days post-infection. The cecum was collected for histopathological and gene expression analyses. Oocyst shedding was significantly reduced in the CAR and ZOL/CAR groups compared with the CT group (<em>p</em> &lt; 0.01). The total oocyst number in the ZOL/CAR group was lower compared with those in the CAR and CT groups (<em>p</em> &lt; 0.05). The lesion score and parasite burden in the CAR and ZOL/CAR groups were lower compared with those in the CT group (<em>p</em> &lt; 0.01). The expression of IFN-λ, IL-17 A, and perforin mRNA in the ZOL/CAR group increased compared with that in the CAR group, whereas the expression of IFN-γ in the ZOL/CAR group decreased. The results suggest that Th1-independent immunity occurs as a local immune response induced by IL-17 A/IL-22-producing γδ T cells, which control <em>E. tenella</em>-induced pathology in the cecum.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105730"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale serosurvey of Chlamydia abortus in zoo ungulates in Spain","authors":"Adrián Beato-Benítez ,&nbsp;Ignacio García-Bocanegra ,&nbsp;Moisés Gonzálvez ,&nbsp;Jessica Villar-Ibáñez ,&nbsp;Débora Jiménez-Martín ,&nbsp;Rosa Martínez-Valverde ,&nbsp;Santiago Borragán ,&nbsp;Manuel de la Riva-Fraga ,&nbsp;David Cano-Terriza","doi":"10.1016/j.rvsc.2025.105731","DOIUrl":"10.1016/j.rvsc.2025.105731","url":null,"abstract":"<div><div><em>Chlamydia abortus</em> is a zoonotic bacterium worldwide distributed, which mainly leads to reproductive disorders in different domestic and wild species. Although <em>C. abortus</em> infection has been widely reported in free-ranging ungulates, information regarding this pathogen in captive wild ungulates remains limited. The aims of the present study were to assess <em>C. abortus</em> exposure in zoo ungulates in Spain and to determine the dynamics of seropositivity in animals sampled longitudinally throughout the study period. Between 2007 and 2023, serum samples from 483 ungulates representing 65 species were collected across 11 zoos from Spain. Additionally, 42 of the 483 animals were longitudinally sampled. Anti-<em>C. abortus</em> antibodies were detected in ten individuals (2.1 %; 95 %CI: 0.8–3.3) using a commercial indirect multi-species ELISA. Seropositivity was found in 9.2 % (6/65) of the species analyzed and in 45.5 % (5/11) of the zoos sampled. Seroprevalence was significantly higher during the period 2019–2020 compared to 2007–2014, 2015–2018 and 2021–2023 (<em>P</em> = 0.013). Three of the 42 longitudinally surveyed animals presented seropositivity throughout the study period. This is the first large-scale serosurvey of <em>C. abortus</em> conducted in zoo ungulates, also describing for the first time exposure to <em>C. abortus</em> in four species, which could expand the host range of this zoonotic bacterium. Our results suggest a limited but widespread circulation of <em>C. abortus</em> in wild ungulates housed in zoos in Spain. Zoo ungulates should be included in surveillance programs for <em>C. abortus</em> to enhance our understanding of the epidemiology of this zoonotic pathogen in anthropized environments under a One Health framework.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105731"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tenogenic potential of tendon-derived mesenchymal stem cells isolated post-mortem: Impact of cryopreservation","authors":"Levoz Marine ,&nbsp;Poirier Wilfried ,&nbsp;Piret Joëlle ,&nbsp;Javaux Justine ,&nbsp;Dubois Axel ,&nbsp;Toppets Vinciane ,&nbsp;Vandenhove Benoît ,&nbsp;Gillet Laurent ,&nbsp;Grobet Luc ,&nbsp;Kaux Jean-François ,&nbsp;Antoine Nadine","doi":"10.1016/j.rvsc.2025.105722","DOIUrl":"10.1016/j.rvsc.2025.105722","url":null,"abstract":"<div><div><em>In situ</em> injection of mesenchymal stem cells appears as a promising treatment of tendinopathies. Tendon-derived mesenchymal stem cells (TDSCs) are widely studied and show a lot of interesting characteristics for clinical use.</div><div>The aim of this study is to confirm the tenogenic potential of cryopreserved TDSCs and to confirm their ability to produce type I and/or type III collagens fibers in culture.</div><div>Tendon-derived mesenchymal stem cells are harvested from the tendon no later than 72 h <em>post-mortem</em>. Their tenogenic potential has been assessed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of different genes involved in tenogenic differentiation. The stemness phenotype of TDSCs has been confirmed by flow cytometry. In order to demonstrate their capacity to synthesize type I and III collagen fibers, a stimulation with Transforming Growth Factor-β1(TGF-β1) has been performed in culture.</div><div>TDSCs comply with the expected phenotype of mesenchymal stem cells as defined by ISCT. All the samples and conditions analyzed by flow cytometry were positive for CD105, CD90, CD29, CD44 and negative for MHC-II. Their tenogenic potential is also confirmed by qRT-PCR. TDSCs are metabolically active cells, showing an ultrastructure reflecting high level of proteins synthesis. Moreover, they are able to synthetize collagen fibers under stimulation by TGF-β1.</div><div>Freshly harvested or cryopreserved TDSCs are involved in the tenogenic pathway and are able to produce an extracellular collagenous network. These properties make them suitable for banking and subsequent clinical use for contributing to tendon repair.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105722"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence factors and strain similarity of Staphylococcus aureus from a dairy farm demonstrate an increased risk of environmental transmission","authors":"Angela Perdomo,&nbsp;Juan Sebastian Solano-Gutierrez,&nbsp;Jonathan Thompson,&nbsp;Alexandra Calle","doi":"10.1016/j.rvsc.2025.105728","DOIUrl":"10.1016/j.rvsc.2025.105728","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is a highly adaptable microorganism that harbors virulence factors driving its pathogenicity, affecting animals and humans. Forty <em>S. aureus</em> isolates were collected from a dairy farm operation and were subjected to several phenomics, genomics, and proteomics tests, including antimicrobial susceptibility, biofilm evaluation, strain typing for isolate-relatedness determination, and enterotoxin production. Further characterization involved whole genome sequencing (WGS) and protein detection using mass spectrometry on a subset of isolates (enterotoxin-positive and enterotoxin-negative). All isolates were susceptible to the tested antibiotics except for Gatifloxacin (GAT), for which 100 % displayed intermediate resistance. All isolates produced biofilms, with a noticeable surge at 48 h compared to 24 h (<em>p</em> &lt; 0.001). Additionally, 10 % of the isolates (<em>n</em> = 4) produced enterotoxins associated with human infections. Strain typing via infrared biotyping (IRBT) categorized the 40 isolates into six distinctive groups, indicating close relationships among <em>S. aureus</em> recovered from different samples, demonstrating a movement of the organism within the farm. Genomic characterization of enterotoxin-positive and enterotoxin-negative isolates identified two sequencing types, ST151 and ST351, accompanied by spa types t529 and t9001. Genomics and proteomics analyses revealed various virulence factors, encompassing cytolytic toxins, immune evasion mechanisms, extracellular matrix (ECM)—binding proteins, proteases, enterotoxins, and chromosomal point mutations. In addition to virulence factors, this study shows how this bacterium moves around the farm, potentially becoming an environmental pathogen, increasing the risk of transmission and infection for humans and animals.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105728"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nationwide serological survey and risk factors for Toxoplasma gondii and Neospora caninum infections in alpacas (Vicugna pacos) and llamas (Lama glama) in Italy","authors":"Elisa Castaldo ,&nbsp;Walter Basso ,&nbsp;Gastón Moré ,&nbsp;Alessia Ciaramelli ,&nbsp;Nicola D'Alessio ,&nbsp;Michele Capasso ,&nbsp;Giovanni Sgroi ,&nbsp;Sara Tonon ,&nbsp;Farwa Humak ,&nbsp;Alessia Gazzonis ,&nbsp;Gereon Schares ,&nbsp;Vincenzo Veneziano","doi":"10.1016/j.rvsc.2025.105729","DOIUrl":"10.1016/j.rvsc.2025.105729","url":null,"abstract":"<div><div><em>Toxoplasma gondii</em> and <em>Neospora caninum</em> are worldwide distributed protozoa, recognized as major causes of economic losses in livestock production due to reproductive failure. Camelids can also serve as intermediate hosts for these protozoa. Research on these parasites in South American Camelids (SAC) in Europe is scarce. Therefore, this study aimed to estimate the seroprevalence and distribution of <em>T. gondii</em> and <em>N. caninum</em> infections in SAC in Italy and investigate risk factors for infection applying a structured online questionnaire filled in by owners. A total of 506 SAC sera (486 alpacas, <em>Vicugna pacos,</em> and 20 llamas, <em>Lama glama</em>) were tested to detect antibodies against <em>T. gondii</em> and <em>N. caninum</em> using enzyme-linked immunosorbent assays (ELISA), and results were confirmed with immunoblot. Seroprevalences of 34.8 % (176/506) for <em>T. gondii,</em> with 33.1 % (161/486) for alpacas and 75.0 % (15/20) for llamas, and 5.7 % (29/506) for <em>N. caninum,</em> with 5.6 % (27/486) for alpacas and 10.0 % (2/20) for llamas were found. Simultaneous presence of antibodies against <em>T. gondii</em> and <em>N. caninum</em> was detected in 3.6 % (18/506) of the samples. The variables older age and llama species were identified as risk factors (<em>p</em> &lt; 0.05) for <em>T. gondii</em>, while the presence of dogs and fertility disorders were associated with <em>N. caninum</em> seropositivity. This study provides the first large-scale serological evidence of <em>T. gondii</em> and <em>N. caninum</em> infections in alpacas and llamas in Italy, highlighting their widespread distribution and contributing valuable data to the national epidemiological scenario. Further studies are needed to evaluate the role of these parasites as cause of abortion.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105729"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre and post fibrate supplementation lipoprotein electrophoresis in hyperlipemic dogs with biliary tree disease","authors":"Eleonora Gori ,&nbsp;Saverio Paltrinieri ,&nbsp;Verena Habermaass ,&nbsp;Ilaria Lippi ,&nbsp;Veronica Marchetti","doi":"10.1016/j.rvsc.2025.105727","DOIUrl":"10.1016/j.rvsc.2025.105727","url":null,"abstract":"<div><div>Hyperlipemia is commonly observed in dogs with biliary tree disease, where cholestasis can both cause and result of hyperlipidemia. Fibrate therapy effectively reduce lipemia in dogs with both primary and secondary hyperlipidemia. This study aimed to investigate whether fenofibrate therapy could alter lipoprotein patterns in hyperlipemic cholestatic dogs. Retrospective cohort study on left-over frozen-stored (−80 °C) serum samples of 25 client-owned dogs with chronic biliary disease with hyperlipidemia (hypercholesterolemia and/or hypertriglyceridemia) that underwent fenofibrate therapy at 4–10 mg/kg once daily (T0) and had a re-check after 4–6 weeks (T1). To be included, dogs with hypercholesterolemia (&gt;280 mg/dL) and/or hypertriglyceridemia (&gt;90 mg/dL) had to present a concurrent increase of two or more between alkaline phosphatase (ALP) &gt;250 U/L, gamma-glutamyl transferase (GGT) &gt;11 U/L and total bilirubin &gt;0.3 mg/dL and abdominal ultrasound alterations suggestive for a chronic biliary tree disease. Pre- and post-treatment serum samples were analysed using lipoprotein electrophoresis. Results showed significant reductions in serum cholesterol (median 293 mg/dL vs. 368 mg/dL) and triglycerides (median 70 mg/dL vs. 181 mg/dL). Lipoprotein analysis revealed a significant increase in HDL percentage (from 51 % to 62.9 %) and reductions in VLDL (from 33.2 % to 18 %) and chylomicrons (from 3.2 % to 1.7 %). These results suggest that fenofibrate is effective in reducing lipemia and improving lipid profiles in cholestatic dogs, helping to limit hepatic injury potentially associated with lipid accumulation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105727"},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}