Research in veterinary science最新文献

{"title":"Loss of viability and impairment of the cell cycle by combining metabolic modulators in canine and feline melanoma cells","authors":"Melisa Denise Pan ,&nbsp;Maria Florencia Arbe ,&nbsp;Gabriela Veronica Salamone ,&nbsp;Gerardo Claudio Glikin ,&nbsp;Liliana María Elena Finocchiaro ,&nbsp;Marcela Solange Villaverde","doi":"10.1016/j.rvsc.2025.105691","DOIUrl":"10.1016/j.rvsc.2025.105691","url":null,"abstract":"<div><div>Despite encouraging advances during the last decade, clinical management of malignant human, canine and feline melanoma continues to be a challenge. Thus, new therapeutic development is required. One of the hallmarks of cancer is metabolic rearrangement, including increased glucose metabolism. This metabolic alteration seems to be involved not only in cell proliferation but also in drug resistance, thus offering potential therapeutic targets. The aim of the present work was to investigate the in vitro effects of a combination of metformin (MET, an antidiabetic drug and OXPHOS inhibitor), 2-deoxyglucose (2DG, an HK inhibitor) and 6-aminonicotinamide (6AN, a G6PDH inhibitor) on two melanoma cell lines, <em>Sc</em> (canine) and <em>Dc</em> (feline) derived from spontaneous tumors. We found that both 2DG and MET treatment significantly decreased the cell viability of both cell lines (<em>p</em> &lt; 0.05) in a concentration-dependent manner, whereas 6AN as monotherapy only significantly affected <em>Sc</em>. In addition, the effect of MET was significantly potentiated (<em>p</em> &lt; 0.05) by the combination with both 2DG and 6AN in both cell lines. MET/2DG and MET/6AN significantly affected the cell cycle and increased the percentage of the subG0 population. These results support further studies to investigate the potential use of these metabolic drugs in a veterinary clinical setting.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105691"},"PeriodicalIF":2.2,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collision tumor of myeloma and infiltrative lipoma in the canine spine","authors":"Aldana Belén Recce ,&nbsp;Luiza Tonietto Mangini ,&nbsp;Vitória Chesini Malvessi ,&nbsp;Glaucia Denise Kommers ,&nbsp;Alexandre Mazzanti ,&nbsp;Diego Vilibaldo Beckmann","doi":"10.1016/j.rvsc.2025.105688","DOIUrl":"10.1016/j.rvsc.2025.105688","url":null,"abstract":"<div><div>Collision tumor is a rare phenomenon in dogs, defined by the coexistence of two distinct neoplasms at the same anatomic site, with no previous reports of multiple myeloma (MM) and infiltrative lipoma collision in dogs. MM is a malignant plasma cell neoplasm, accounting for less than 1 % of canine malignant tumors, primarily affecting the axial skeleton with osteolytic lesions. Infiltrative lipomas can invade adjacent tissues, including the spinal canal, leading to neurological deficits. This study describes a case of tumor collision between MM and infiltrative lipoma in the spine of a 14-year-old mixed-breed dog with a three-month history of lumbosacral swelling and progressive neurological deficits, emphasizing the importance of a thorough diagnostic approach for effective treatment. Myelography revealed extensive osteolysis of the fourth, fifth and sixth lumbar vertebrae, along with a sudden contrast interruption and a filling defect at L6, prompting surgical intervention for tumor excision and cauda equina decompression. During surgery, meticulous tissue sampling from the subcutaneous layer to the epidural fat enabled histopathological identification of MM infiltrating beyond L6 and coexisting with an infiltrative lipoma. MM was confirmed by monoclonal gammopathy detected via serum protein electrophoresis. Following diagnosis, treatment with melphalan and prednisone resulted in pain resolution and reduced serum gamma globulin levels. After 925 days of follow-up, the dog remains clinically stable with no recurrence of clinical signs. This case underscores the critical role of accurate diagnosis in identifying tumor collisions, guiding targeted treatment, and improving survival outcomes.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105688"},"PeriodicalIF":2.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus suis serotypes 2, 1/2, 1, and 14 isolated from the brain of pigs dead with neurological symptoms or sudden death in Italian farms.","authors":"Silvia Faccini ,&nbsp;Luciano Gintoli ,&nbsp;Mario D'Incau ,&nbsp;Elena Cursio ,&nbsp;Carlo Rosignoli","doi":"10.1016/j.rvsc.2025.105683","DOIUrl":"10.1016/j.rvsc.2025.105683","url":null,"abstract":"<div><div>The <em>Streptococcus suis</em> serotypes 2, and 14 are zoonotic pathogens in swine and present a capsular polysaccharide structure closely related to serotypes 1 and 1/2. Most molecular methods applied in veterinary diagnostic laboratories do not detect the single nucleotide polymorphism (SNP) that differentiates the serotypes 2 from 1/2, and 1 from 14. Consequently, epidemiological data is lacking. A collection of strains isolated between 2019 and 2024 from the brain of diseased pigs, and previously classified as 2–1/2 or 1–14, was analysed using three molecular methods able to detect of the SNP distinguishing the four serotypes. The study revealed that 57 % of those formerly classified as serotype 2–1/2 actually belong to serotype 1/2, while no serotype 14 was found among the strains classified as 1–14. The study also evaluates the feasibility of the routine application of these PCR-based methods for SNP detection, emphasizing the importance for accurate and rapid serotype identification to aid vaccine production and epidemiology.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105683"},"PeriodicalIF":2.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial psychometric evaluation of the canine brief pain inventory in a Danish sample of dogs with pain related to osteoarthritis","authors":"Kathrine Højte Dahl,&nbsp;Mikkel Hammer Hannesbo,&nbsp;Anders Christoffer Kruse,&nbsp;James Edward Miles,&nbsp;Michelle Brønniche Møller Nielsen","doi":"10.1016/j.rvsc.2025.105685","DOIUrl":"10.1016/j.rvsc.2025.105685","url":null,"abstract":"<div><h3>Introduction</h3><div>Osteoarthritis (OA) is the most diagnosed arthropathy in dogs. Since the characteristic manifestation of OA is pain, clinical metrology instruments for OA should detect observable outcomes related to OA pain and be validated in the translated form. This study aimed to validate a Danish translated version of Canine Brief Pain Inventory (CBPI) in a cohort of dogs diagnosed with OA.</div></div><div><h3>Methods</h3><div>The CBPI was translated into Danish. Data of clinically healthy dogs were compared with data of dogs with OA, and the translation was analysed for construct validity and internal consistency. Construct validity was tested with an exploratory factor analysis by principal component analysis, while internal consistency was estimated by Cronbach's α coefficient.</div></div><div><h3>Results and conclusions</h3><div>The Danish version of CBPI was completed by owners of 72 dogs with OA and 21 owners of clinically heathy dogs. Dogs with OA had significantly higher CBPI scores than healthy dogs, demonstrating the Danish CBPI's discriminant ability. An identified two-factor model accounted for 77.7 % of the total variance. Good internal consistency was identified across the instrument with high Cronbach's α levels: 0.95 for the total CBPI, 0.93 for the first factor (items 1–6) and.0.88 for the second factor (items 7–10).</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105685"},"PeriodicalIF":2.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel identification of telocytes in the skin of the desert tortoise (Testudo graeca): A correlative study using semithin section, transmission electron microscopy, and immunofluorescence","authors":"Samar M. Ez Elarab ,&nbsp;Hagar M. Mohamed ,&nbsp;Samir A.A. El-Gendy ,&nbsp;Ahmed A. El-Mansi ,&nbsp;Mamdouh B. Eldesoqui ,&nbsp;Amal F. Dawood ,&nbsp;Mohamed A.M. Alsafy ,&nbsp;Ahmed M. Rashwan","doi":"10.1016/j.rvsc.2025.105689","DOIUrl":"10.1016/j.rvsc.2025.105689","url":null,"abstract":"<div><div>This study comprehensively investigates the presence and characteristics of telocytes in the skin of desert tortoises, employing a multifaceted approach that includes light microscopy, transmission electron microscopy (TEM), and immunofluorescence analyses. A groundbreaking revelation is made as our study, for the first time, unveils the existence of telocytes in the skin of the Greek tortoise. The intricate architecture of the skin, comprising scales, a keratin layer, an epidermis, a dermis, and a hypodermis, is meticulously examined. The epidermis, marked by a stratified epithelium and a substantial keratin layer, features a corrugated basement membrane. Notably, the dermis is a significant site housing telocytes, identified by their distinctive rod-shaped morphology and extensive cytoplasmic processes, including a thick podom and a thin podomere containing nanovesicles. Extracellular nanovesicles play a crucial role in facilitating communication between telocytes and melanophores. Utilizing CD34, PDGFRα, and Vimentin markers, we observe the abundance and intensity of CD34, particularly in the central dermis, and the widespread presence of PDGFRα throughout the dermis. Although less abundant near the epidermis and hypodermis, Vimentin increases in the central dermis with reduced intensity compared to CD34 and PDGFRα. Co-expression analyses reveal robust associations between CD34 and PDGFRα, highlighting them as dominant markers in telocytes. This suggests their potential significance in supporting the tortoise's skin health and resilience in harsh desert conditions. The understanding of these molecular dynamics provides insights into the adaptive strategies employed by desert tortoises, emphasizing the therapeutic relevance of these markers in enhancing skin health in challenging environments.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105689"},"PeriodicalIF":2.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial effects of probiotics on dysbiosis of gut microbiota induced by antibiotic treatment in healthy dogs","authors":"Sung-Jae Kim ,&nbsp;Hee-Chun Chung ,&nbsp;Soo-Yeon Park ,&nbsp;Jae-Myun Lee ,&nbsp;Jeong-Hee Han","doi":"10.1016/j.rvsc.2025.105674","DOIUrl":"10.1016/j.rvsc.2025.105674","url":null,"abstract":"<div><div>The gut microbiota plays a crucial role in maintaining host health. While numerous studies have explored the impact of antibiotics on the gut microbiota in humans, limited research has examined how antibiotics affect the gut microbiome in dogs. This study investigated the effects of antibiotic treatment on the gut microbiota of dogs and assessed whether probiotic supplementation could prevent antibiotic-induced dysbiosis. Fourteen healthy young dogs undergoing castration were included in the study. All dogs received a single injection of cefovecin immediately after surgery. The probiotics group (7 dogs) was given a probiotic complex daily starting on the day of surgery and continuing for two weeks, while the non-probiotics group (7 dogs) received no supplementation. Fecal samples were collected on the day of surgery and two weeks later during the follow-up visit for suture removal for microbiome analysis. In microbial diversity analysis, α-diversity was significantly higher in the probiotic-supplemented group compared to the non-probiotics group (<em>p</em> &lt; 0.05). β-diversity analysis revealed significant differences in microbial community composition in the non-probiotics group after antibiotic treatment (<em>p</em> &lt; 0.05), while no significant differences were observed in the probiotics group. Relative abundance analysis indicated that <em>Clostridioides</em>, a marker of antibiotic-induced dysbiosis, significantly increased in dogs without probiotics after antibiotic treatment (<em>p</em> &lt; 0.05). In contrast, <em>Butyricicoccus</em>, a butyrate-producing bacterium with gut health benefits, was significantly enriched in the probiotics group (<em>p</em> &lt; 0.05). These findings suggest that probiotic supplementation supports healthier gut microbiome recovery following antibiotic treatment and highlights its potential to enhance gut microbiota restoration and mitigate gut dysbiosis caused by antibiotics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105674"},"PeriodicalIF":2.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac biomarkers N-terminal fragment of the prohormone B-type natriuretic peptide and cardiac troponin I for characterization of the cardiac disease phenotype of the English Bull Terrier","authors":"C. Chompoosan ,&nbsp;J. Koch,&nbsp;A.S. Schrøder,&nbsp;M.B.T. Bach,&nbsp;J.L. Willesen,&nbsp;R. Langhorn","doi":"10.1016/j.rvsc.2025.105686","DOIUrl":"10.1016/j.rvsc.2025.105686","url":null,"abstract":"<div><div>The N-terminal fragment of prohormone B-type natriuretic peptide (NT-proBNP) and cardiac troponin I (cTnI) contribute information regarding cardiac load and function and myocardial injury, respectively, to the clinical work-up of dogs with heart disease. The English bull terrier (BT) has a predisposition to a complex cardiac phenotype. This prospective case-control study aimed to further characterize this phenotype through cardiac biomarker evaluation.</div><div>Fifteen healthy BT (group 1) and 50 BT with various heart disease presentations (groups 2–4) were included. The main presentations were MVD (45 dogs) and MS (13 dogs). 16/50 dogs had echocardiographic evidence of cardiac remodeling: 7 with either increased left atrial (LA) or left ventricular (LV) volumes (group 3) and 9 with increased LA and LV volumes (group 4).</div><div>Cardiac biomarkers were not significantly different between dogs with disease subtypes MVD or MS versus dogs with other disease presentations. When evaluated in relation to remodeling, median NT-proBNP and cTnI in group 4 (1004 pmol/L, 0.09 ng/mL) were significantly higher than in group 1 (257 pmol/L, 0.02 ng/mL), group 2 (300 pmol/L, 0.026 ng/mL) and group 3 (250 pmol/L, 0.02 ng/mL). There was significant discriminatory ability for both biomarkers between group 4 and groups 1, 2 or 3. Furthermore, cTnI significantly differentiated dogs with any degree of remodeling (groups 3 + 4) from those without (groups 1 + 2).</div><div>The cardiac disease phenotype of the BT is not associated with a unique cardiac biomarker profile. Rather, biomarker levels are similar to those reported in other breeds with valvular heart disease.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105686"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in the tear film lipid layer by age in healthy brachycephalic dogs","authors":"Alessandro Cirla ,&nbsp;Valentina Andreani ,&nbsp;Angelo Pasquale Giannuzzi ,&nbsp;Laura Ventura ,&nbsp;Giovanni Barsotti","doi":"10.1016/j.rvsc.2025.105684","DOIUrl":"10.1016/j.rvsc.2025.105684","url":null,"abstract":"<div><div>This cross-sectional study evaluates the morphological and qualitative changes in the meibomian glands (MGs) and tear film lipid layer (TFLL) associated with aging and sex in canine brachycephalic breeds. One hundred and eleven brachycephalic dogs with normal eyes were included. Dogs were divided into three age groups: ≤4 years (G1), &gt;4 and &lt; 10 years (G2) and ≥ 10 years (G3). Schirmer test I (STT I), upper eyelid MGs noncontact infrared meibography (NIM), tear interferometry (TFI) and tear film breakup time (TFBUT) were performed in each eye. Loss of MGs and lid margin abnormalities (LMAs), as well as TFI patterns were scored from 0 to 3 depending on severity. Normality was assessed with the Shapiro-Wilk test. The Kruskal-Wallis test was used for the analysis of variance among groups. Pearson's or Spearman's correlation coefficient was used to assess the correlation between MG loss and STT I values. Significance was set at <em>p</em> &lt; 0.05. Forty-four Shih-Tzus, 37 Pugs and 30 French Bulldogs were enrolled in the study. Median age was 39.5 months (range 30–48 months) in G1, 94.5 months (range 65–118 months) in G2, 154.5 months (range 120–201 months) in G3. Loss of MGs was statistically higher in G2 and G3 (<em>p</em> &lt; 0.001) and was associated with a reduction in TFI scores (p &lt; 0.001) and STT I (p &lt; 0.001). Loss of MGs was then negatively correlated with TFBUT. No statistical differences were associated with sex (<em>p</em> &gt; 0.05) or breed (p &gt; 0.05). The tear film lipid layer appears to be negatively affected by age in brachycephalic dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105684"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereological and morphometric insights into epididymal development in domestic cats (Felis silvestris catus) from 6 to 48 months","authors":"P. Salinas,&nbsp;D. Escobar","doi":"10.1016/j.rvsc.2025.105690","DOIUrl":"10.1016/j.rvsc.2025.105690","url":null,"abstract":"<div><div>This study characterizes age-related morphometric and morphological changes in the epididymis of domestic cats (<em>Felis silvestris catus</em>) from puberty to adulthood (6 to 48 months), emphasizing its essential role in sperm maturation and storage—key processes for male fertility. A total of 42 epididymides were analyzed using histological staining (hematoxylin-eosin) and stereological quantification through the STEPanizer software. Morphometric analyses revealed an age-dependent increase in the diameter of the epididymal duct and epithelial height in the <em>caput</em>, whereas the <em>cauda</em> exhibited a progressive reduction in epithelial height, possibly reflecting adaptations in sperm storage capacity during sexual maturation. Morphological observations showed the presence of intraepithelial cysts in cats aged 24 to 48 months, along with the consistent detection of spermatozoa in all regions and age groups. Stereological findings indicated an increased volumetric density (%VV) of the ductal epithelium, particularly in the <em>caput</em> between 6 and 12 months of age, supporting the influence of androgenic activity on regional epididymal maturation. These changes suggest dynamic, age-related structural remodeling of the epididymal parenchyma, especially in epithelial and luminal components. While this cross-sectional study—conducted during the southern hemisphere spring—provides valuable insights into epididymal development, its design limits the establishment of causal relationships between age and histological changes. Future longitudinal studies examining hormonal modulation of epididymal maturation in domestic cats are encouraged. Overall, these findings contribute foundational knowledge of feline reproductive anatomy and underscore the importance of the epididymis as a hormonally responsive organ central to male fertility.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105690"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from African swine fever virus-infected pigs mediate immune responses through NF-κB and JAK-STAT signaling pathways","authors":"Suyeon Kang ,&nbsp;Thi Hao Vu ,&nbsp;Chaeeun Kim ,&nbsp;Anh Duc Truong ,&nbsp;Yeong Ho Hong","doi":"10.1016/j.rvsc.2025.105687","DOIUrl":"10.1016/j.rvsc.2025.105687","url":null,"abstract":"<div><div>African swine fever (ASF) virus (ASFV) is an infectious disease that affects the pig industry, causing up to 85 % morbidity and 100 % mortality. To date, there are no available vaccines against ASFV. Exosomes are extracellular vesicles that are released from most cell types. Exosomes carry components such as nucleic acids, lipids, and proteins that play a vital role in cell-to-cell communication. This study investigated the effect of exosomes derived from the serum of ASFV-infected pigs on a porcine macrophage cell line. Exosomes derived from the serum of pigs infected with ASFV contained ASFV structural proteins (p30 and p72). Expression levels of interferon (IFN)-α, IFN-γ, IL-6, and CXCL8 in porcine macrophage cells were affected by exposure to exosomes derived from the serum of ASFV-infected pigs. Nuclear factor-κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway play an important role in the immune response to ASFV infection. Exosomes derived from ASFV-infected pigs affected mRNA and protein levels of NF-κB, tank binding kinase 1 (TBK1), JAK1, JAK2, and STAT1, suggesting that exosomes derived from ASFV-infected pigs mediate antiviral response by modulating the expression of inflammatory cytokines and activity of the NF-κB and JAK-STAT signaling pathways. The present study provides novel information about the immunomodulatory effects of exosome derived from pigs infected with ASFV, improving our understanding of ASFV pathogenesis and the host immune response to ASFV infection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"191 ","pages":"Article 105687"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}