γδ T cells induced by zoledronate under macrophage-depleted conditions reduce disease severity and parasite number in Eimeria tenella-infected chicks

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-05-29 DOI:10.1016/j.rvsc.2025.105730

Quan Viet Le , Makoto Matsubayashi , Toshimitsu Hatabu

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Abstract

Disease severity and local immune responses mediated by Zoledronate (ZOL)-induced γδ T cells were evaluated in chicks infected with E. tenella under macrophage-depleted conditions. Three groups of White Leghorns, including a negative control group (CT), carrageenan-treated group (CAR), and both ZOL and CAR-treated group (ZOL/CAR), were infected orally with E. tenella sporulated oocysts (1 × 10⁴ oocysts/chick) at 14 days of age. Fecal oocyst shedding was assessed at 4–15 days post-infection. The cecum was collected for histopathological and gene expression analyses. Oocyst shedding was significantly reduced in the CAR and ZOL/CAR groups compared with the CT group (p < 0.01). The total oocyst number in the ZOL/CAR group was lower compared with those in the CAR and CT groups (p < 0.05). The lesion score and parasite burden in the CAR and ZOL/CAR groups were lower compared with those in the CT group (p < 0.01). The expression of IFN-λ, IL-17 A, and perforin mRNA in the ZOL/CAR group increased compared with that in the CAR group, whereas the expression of IFN-γ in the ZOL/CAR group decreased. The results suggest that Th1-independent immunity occurs as a local immune response induced by IL-17 A/IL-22-producing γδ T cells, which control E. tenella-induced pathology in the cecum.

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巨噬细胞缺失条件下唑来膦酸钠诱导的γδ T细胞可降低柔嫩艾美耳球虫感染雏鸡的疾病严重程度和寄生虫数量

在巨噬细胞缺失的条件下，研究了唑来膦酸钠（ZOL）诱导的γδ T细胞介导的疾病严重程度和局部免疫反应。采用阴性对照组（CT）、卡拉胶处理组（CAR）、ZOL和CAR处理组（ZOL/CAR）三组白来角鸡，在14日龄时经口感染tenella芽孢卵囊（1 × 104个/只）。感染后4-15天评估粪卵囊脱落情况。收集盲肠进行组织病理学和基因表达分析。与CT组相比，CAR组和ZOL/CAR组的卵囊脱落明显减少(p <；0.01)。ZOL/CAR组总卵囊数低于CAR组和CT组(p <；0.05)。与CT组相比，CAR组和ZOL/CAR组的病变评分和寄生虫负担均较低(p <；0.01)。与CAR组相比，ZOL/CAR组IFN-λ、IL-17 A和perforin mRNA的表达升高，而IFN-γ的表达降低。结果表明，th1非依赖性免疫是由产生IL-17 a / il -22的γδ T细胞诱导的局部免疫反应，该细胞控制着肠内纤毛虫诱导的病理。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.