Tenogenic potential of tendon-derived mesenchymal stem cells isolated post-mortem: Impact of cryopreservation

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-05-29 DOI:10.1016/j.rvsc.2025.105722

Levoz Marine , Poirier Wilfried , Piret Joëlle , Javaux Justine , Dubois Axel , Toppets Vinciane , Vandenhove Benoît , Gillet Laurent , Grobet Luc , Kaux Jean-François , Antoine Nadine

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引用次数: 0

Abstract

In situ injection of mesenchymal stem cells appears as a promising treatment of tendinopathies. Tendon-derived mesenchymal stem cells (TDSCs) are widely studied and show a lot of interesting characteristics for clinical use.

The aim of this study is to confirm the tenogenic potential of cryopreserved TDSCs and to confirm their ability to produce type I and/or type III collagens fibers in culture.

Tendon-derived mesenchymal stem cells are harvested from the tendon no later than 72 h post-mortem. Their tenogenic potential has been assessed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of different genes involved in tenogenic differentiation. The stemness phenotype of TDSCs has been confirmed by flow cytometry. In order to demonstrate their capacity to synthesize type I and III collagen fibers, a stimulation with Transforming Growth Factor-β1(TGF-β1) has been performed in culture.

TDSCs comply with the expected phenotype of mesenchymal stem cells as defined by ISCT. All the samples and conditions analyzed by flow cytometry were positive for CD105, CD90, CD29, CD44 and negative for MHC-II. Their tenogenic potential is also confirmed by qRT-PCR. TDSCs are metabolically active cells, showing an ultrastructure reflecting high level of proteins synthesis. Moreover, they are able to synthetize collagen fibers under stimulation by TGF-β1.

Freshly harvested or cryopreserved TDSCs are involved in the tenogenic pathway and are able to produce an extracellular collagenous network. These properties make them suitable for banking and subsequent clinical use for contributing to tendon repair.

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死后分离的肌腱源间充质干细胞的成腱潜能：冷冻保存的影响

原位注射间充质干细胞似乎是一种很有前途的肌腱病治疗方法。肌腱源性间充质干细胞（tdsc）被广泛研究，并显示出许多有趣的临床应用特性。本研究的目的是确认冷冻保存的tdsc的成腱潜力，并确认它们在培养中产生I型和/或III型胶原纤维的能力。肌腱来源的间充质干细胞不迟于死后72小时从肌腱中采集。通过定量逆转录酶聚合酶链式反应（qRT-PCR）对不同基因参与的腱鞘分化进行了评估。流式细胞术证实了tdsc的干性表型。为了证明它们合成I型和III型胶原纤维的能力，我们在培养中进行了转化生长因子-β1（TGF-β1）的刺激。tdsc符合ISCT定义的间充质干细胞的预期表型。流式细胞术分析的所有样品和条件CD105、CD90、CD29、CD44均呈阳性，MHC-II呈阴性。qRT-PCR也证实了它们的致腱鞘潜能。tdsc是代谢活跃的细胞，其超微结构反映了高水平的蛋白质合成。并且在TGF-β1的刺激下能够合成胶原纤维。新鲜收获或冷冻保存的tdsc参与肌腱生成途径，并能够产生细胞外胶原网络。这些特性使其适合储存和随后的临床应用，有助于肌腱修复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.