Research in veterinary science最新文献

{"title":"Behavioral and orthopedic issues in geldings with Postcastration adhesions: A retrospective study of 35 cases","authors":"Natalia Domańska-Kruppa ,&nbsp;Philippe Benoit ,&nbsp;Katarzyna Żukiewicz-Benoit ,&nbsp;Małgorzata Domino ,&nbsp;Katarzyna Kliczkowska ,&nbsp;Monika Szubart ,&nbsp;Elżbieta Stefanik","doi":"10.1016/j.rvsc.2025.105910","DOIUrl":"10.1016/j.rvsc.2025.105910","url":null,"abstract":"<div><h3>Background</h3><div>Limited data is available on the diagnostic and therapeutic methods for pathologies caused by scarring after castration. It is well recognized that specific behaviors or untypical hindlimb lameness may be observed in geldings with gelding scars and adhesions caused by spermatic cord remnants after castration</div></div><div><h3>Objectives</h3><div>To describe how to diagnose movement disorders associated with excessive adhesions caused by spermatic cord remnant after castration and to present a successful surgical treatment method.</div></div><div><h3>Methods</h3><div>To assess the impact of gelding scars on horses' movement and behavior, medical record data were collected from 35 geldings who underwent surgical treatment for excessive gelding scars. Following non-specific behavioral and clinical signs were observed: reluctance to move forward, bucking, resistance to work, lumbar pain, gluteus spasm, tensed iliopsoas muscles, inguinal pain, and unexplained hind limb irregularity. Presented geldings underwent surgical treatment.</div></div><div><h3>Results</h3><div>Histopathological evaluation of the removed tissues showed evidence of chronic inflammation. The length of the removed spermatic cord remnants ranged between 6 and 24 cm. The detailed method for qualifying horses for surgical procedures and the outcomes of surgical treatment have been shown to be effective in 94.3 % of cases.</div></div><div><h3>Main limitations</h3><div>Histological examination was performed on only 4 out of 35 cases. The study did not include a control group. Symptom severity was not measured using a validated scale.</div></div><div><h3>Conclusions</h3><div>Horses involved in sports and leisure activities may experience unrecognized pain-related changes in their gait and behavior due to adhesions following castration. However, surgical intervention for these postcastration adhesions and inflammatory tissues is a safe and effective solution to restore their well-being.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105910"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocular tolerance and tear film pharmacokinetics of 1 % penciclovir cream in cats","authors":"O. Pe'er ,&nbsp;S.M. Thomasy ,&nbsp;E. Feuer ,&nbsp;H.K. Knych ,&nbsp;R. Ofri ,&nbsp;L. Sebbag","doi":"10.1016/j.rvsc.2025.105909","DOIUrl":"10.1016/j.rvsc.2025.105909","url":null,"abstract":"<div><div>Feline herpesvirus type 1 (FHV-1) is a leading cause of ocular surface disease in cats, often requiring prolonged antiviral therapy. While oral famciclovir is effective, its use is limited by poor bioavailability and compliance challenges. This study evaluated the ocular tolerance and tear film pharmacokinetics of topical 1 % penciclovir cream (Fenlips®), an FDA-approved dermatologic formulation repurposed for ophthalmic use in cats. A retrospective survey of owners whose cats were treated with Fenlips® revealed high levels of satisfaction, with 85 % reporting no adverse effects and 80 % rating the treatment as effective or highly effective. To further assess safety and pharmacokinetics, two prospective studies were conducted in seven healthy domestic cats. In the first, Fenlips® was applied to one eye and artificial tears (DuraTears®) to the other eye for 10 days. No significant differences were observed between eyes in clinical scores, tear production, or tear film stability. Corneal sensitivity increased significantly in Fenlips®-treated eyes. In the second study, tear fluid samples collected over 24 h revealed that penciclovir concentrations remained above the minimal inhibitory concentration (0.30 μg/ml) for FHV-1 for over 8 h on average, with a terminal half-life of 8.75 h. These findings indicate that Fenlips® is well tolerated and achieves pharmacologically relevant concentrations in the tear film, supporting its potential use as a twice-daily topical treatment for herpetic keratoconjunctivitis in cats.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105909"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a bacillus-based biological modulator on Salmonella Heidelberg contamination and cecal microbiota in chickens.","authors":"Jeferson Luiz Richter ,&nbsp;Bruna Gabriela Gheller Kaefer ,&nbsp;Emanoelli Aparecida Rodrigues dos Santos ,&nbsp;Leonardo Ereno Tadielo ,&nbsp;Jhennifer Arruda Schmiedt ,&nbsp;Dany Alberto Mesa Fiaga ,&nbsp;Vinicius Cunha Barcellos ,&nbsp;Geraldo Camilo Alberton ,&nbsp;Luciano dos Santos Bersot","doi":"10.1016/j.rvsc.2025.105914","DOIUrl":"10.1016/j.rvsc.2025.105914","url":null,"abstract":"<div><div>Broiler litter can serve as a favorable environment for the survival and propagation of pathogens, such as <em>Salmonella</em>, creating negative impacts at all stages of the production chain. Given this, it is essential to seek strategies aiming to effectively control litter contamination. With the growing focus of the poultry chain on sustainability, biological treatments, like Environmental Biological Modulation (BM), have been gaining prominence. In the present study, we evaluated the in-situ effect of a <em>Bacillus</em> spp.-based BM on the contamination rates of <em>Salmonella</em> spp. in the poultry litter and the digestive tract of challenged birds, as well as its influence on the composition of the intestinal microbiota. The results showed a strong influence of the BM in reducing the frequency of positivity and the average pathogen count, both in the litter and in the birds' cecal content. Moreover, it was possible to identify significant differences in the taxonomic composition and abundance of the microbiota in the presence of the modulator. Taxa such as <em>Streptococcus</em>, <em>Lactobacillus</em>, <em>Alistipes,</em> and <em>Christensenella minuta</em> showed notable variations in the presence of BM or <em>Salmonella</em> contamination, being highlighted in both the analyses of classification models and network analysis. These results are important for advancing the understanding of biological tools and their contribution to sanitary and environmental control strategies. This study provides novel insights into the interplay between environmental modulation and cecal microbiota dynamics during a <em>S.</em> Heidelberg challenge.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105914"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indirect assessment of autonomic nervous system balance for prediction of hemorrhage: A preclinical study in a porcine model","authors":"Mathieu Magnin ,&nbsp;Amandine Chapelle ,&nbsp;Stéphane Junot ,&nbsp;Bernard Allaouchiche ,&nbsp;Timothée Schuhler ,&nbsp;Romain Lac ,&nbsp;Valentin Bondoux ,&nbsp;Guillaume L. Hoareau ,&nbsp;Mark Kim ,&nbsp;Tatiana Victoni ,&nbsp;Céline Pouzot-Nevoret","doi":"10.1016/j.rvsc.2025.105915","DOIUrl":"10.1016/j.rvsc.2025.105915","url":null,"abstract":"<div><div>Hemorrhage is a common and significant complication of trauma or surgical procedures. Rapid and accurate assessment of a patient's condition during hemorrhage is crucial for management. The primary objective was to describe pupillometry parameters and parasympathetic tone activity (PTA) during hemorrhage. The secondary objective was to develop a model to predict blood losses.</div><div>In this preclinical study, 15 pigs were used. After anesthesia, 35 mL/kg of blood was removed over 1 h. Hypovolemia was maintained for 90 min. Indirect assessment of autonomic nervous system balance was based on the monitoring of heart rate (HR), blood pressure (BP), shock index (SI), PTA and pupil diameter (PD). PD and the pupillary light reflex (PLR) were measured using a portable pupillometer.</div><div>HR and SI significantly increased during hemorrhage, whereas BP decreased. PD increased significantly during hemorrhage (+0.03 mm/min, [0.01; 0.04]; then +0.01 mm/min, [0.01; 0.02]) and the hypovolemia period (+0.004 mm/min, [0.002; 0.005]). PLRs were absent in 251/254 measurements. Large variations in PTA parameters were observed over time, which prevented statistical analysis. The best models predicted blood losses with a mean absolute error ranging from 3.61 to 3.67 mL/kg.</div><div>In conclusion, hemorrhage was associated with an increased PD potentially influenced by anesthesia. The selected models allowed reasonable prediction of blood losses with a slight tendency to overestimate. The study provides proof of concept for the use of this kind of models to predict blood losses. The results must be confirmed in other contexts: with different species, other anesthetic protocols, or different surgical procedures.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105915"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a one-step rapid immunochromatographic test kit for detecting bovine mastitis-causing Staphylococcus aureus and streptococci","authors":"Yusaku Tsugami ,&nbsp;Aoi Sugiyama ,&nbsp;Yu Ando ,&nbsp;Kotaro Oda ,&nbsp;Koji Maehana ,&nbsp;Shintarou Hayashi ,&nbsp;Kento Fujii ,&nbsp;Yuya Nagasawa","doi":"10.1016/j.rvsc.2025.105911","DOIUrl":"10.1016/j.rvsc.2025.105911","url":null,"abstract":"<div><div>Major pathogens causing mastitis, such as <em>Staphylococcus aureus</em> and streptococci can be transmitted between cows. Specific antibiotics are needed to exterminate these pathogens. However, bacterial culture for pathogen identification requires at least 18 h. Thus, a rapid diagnostic method is needed for identifying pathogens. A previously reported immunochromatographic strip (ICS) test, based on a lateral flow assay, uses individual lysis buffers for different mastitis-causing pathogens, which may produce technical errors. In this study, a one-step rapid ICS test kit using simple procedures to detect bovine mastitis-causing <em>S. aureus</em> and streptococci was developed. In the ICS test version (ver.) 2, the new test-strip design integrated bacteriolytic enzymes, while a common lysis buffer (consisting of 3 % Triton X-100, 0.5 % sodium N-lauroylsarcosinate, and 0.15 % Zwittergent 3–12) simplified the testing procedure. In refrigerated fresh milk samples from cows with clinical mastitis (<em>n</em> = 77), the sensitivity, specificity, and κ statistic values (the agreement between the results of the ICS test and bacterial culture) of the ICS test ver. 2 for <em>S. aureus</em> were 0.864, 0.909, and 0.752, respectively, and those for streptococci were 0.778, 0.956, and 0.700, respectively, as compared to traditional bacterial culture. Thus, <em>S. aureus</em> and streptococci can be detected in the milk with a simple one-step procedure. Some cross-reactivity with non-aureus staphylococci was observed in ICS test ver. 2 for <em>S. aureus</em>. Nonetheless, the improved ICS test kit may serve as an effective detection tool for staphylococci and streptococci mastitis, supporting timely and appropriate control of mastitis on farms.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105911"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine ephemeral fever: From underestimated illness to emerging threat - A review of pathogenesis, economic impact, and future control strategies","authors":"Shruti Pyasi ,&nbsp;Neha Rani Sahu ,&nbsp;Umasish Mohanty ,&nbsp;Debasis Nayak","doi":"10.1016/j.rvsc.2025.105904","DOIUrl":"10.1016/j.rvsc.2025.105904","url":null,"abstract":"<div><div>Bovine ephemeral fever (BEF) is an arthropod-borne viral disease caused by the bovine ephemeral fever virus (BEFV) of the genus <em>Ephemeroviru</em>s in the family <em>Rhabdoviridae</em>. BEF affects domestic and wild ruminants, particularly cattle and water buffaloes. BEF outbreaks are seasonal in Africa, Asia, the Middle East, and Australia, aligning with the summer and autumn seasons in temperate zones and during the monsoons in tropical areas. Vectors, like biting midges (Culicoides spp.) and mosquitoes (Anopheles and Culex spp.), facilitate BEFV transmission. The disease presents a spectrum of clinical signs, from mild febrile illness and lameness to severe symptoms. These severe signs, including loss of the swallowing reflex, tachycardia, emphysema, and ruminal atony, can escalate to neurological disorders and paralysis. With morbidity rates as high as 80–100 %, BEF has a significant impact on livestock productivity. While BEF is clinically widespread, comprehensive genomic data is limited. This is due to lack of systematic surveillance and sequencing capability in endemic regions. Additionally, the acute and transient nature of BEFV infection, with short viremia phase of lasting about 2–8 days, makes it difficult to timely obtain high-quality viral RNA for generating full-length genomes for sequencing. Symptomatic treatments involve non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and corticosteroids, yet no licensed antiviral drugs exist. In this review, we highlight BEFV's epizootiology, economic impact, molecular characterization, immune responses, and control measures, aiming to update the current understanding of BEFV for global prevention and eradication efforts.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105904"},"PeriodicalIF":1.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic granulomatosis associated with hairy vetch toxicosis in a cattle herd","authors":"Viju V. Pillai,&nbsp;Mario F. Sola,&nbsp;Stephen B. Hooser","doi":"10.1016/j.rvsc.2025.105905","DOIUrl":"10.1016/j.rvsc.2025.105905","url":null,"abstract":"<div><div>An outbreak of severe nodular to diffuse dermatitis affecting black yearling cattle occurred on a northeast Colorado farm. The outbreak took place during early summer and resulted in the death of nineteen animals, while several others exhibited symptoms of declining appetite, rough, scaly skin, and chronic weight loss. Physical examination findings included poor body condition, hyperthermia, pruritus, and superficial lymphadenopathy. The outbreak was restricted to animals grazing on abandoned farmland, and no improvement was noted following symptomatic treatment. Postmortem examination revealed marked lymphadenopathy with pale raised foci and streaks on the external and cut surfaces of the skin, kidney, spleen, and liver. Histologic evaluation of skin sections showed severe nodular to diffuse granulomatous dermatitis with eosinophils and multinucleated giant cells. Similar eosinophilic granulomatous inflammation was also observed in sections of the kidneys, liver, and spleen. These results were consistent with systemic granulomatous disease, highly suggestive of hairy vetch toxicosis, as the affected animals had been grazing on a pasture containing hairy vetch.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105905"},"PeriodicalIF":1.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics combined with 16S rDNA revealed the effects of low protein diet on rumen microbiome structure, nitrogen utilization rate and differential metabolites in feces and urine of dairy cows","authors":"Yaqiong Ren ,&nbsp;Caijuan Yue ,&nbsp;Wenzhuo Wang ,&nbsp;Li Cai ,&nbsp;Yanming Wang ,&nbsp;Xiaojing Liu ,&nbsp;Depeng Li ,&nbsp;Qiao'’e Zhang","doi":"10.1016/j.rvsc.2025.105901","DOIUrl":"10.1016/j.rvsc.2025.105901","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;The use of low-protein diets on the basis of amino acid balance is conducive to optimizing feed formulations, reducing production costs, and improving economic efficiency. Aims of the study: Research objective: This experiment aims to conduct a feeding test by selecting 40 Holstein dairy cows and randomly dividing them into 2 groups, with 20 cows in each group and 20 replicates in each group, and one cow in each replicate. The effects of reducing dietary protein levels, adding RP-Lys and RP-Met on rumen microbial diversity, nitrogen metabolism and different metabolites in feces and urine of dairy cows were investigated by using 16S rDNA and metabolomics. To provide a certain theoretical basis for guiding dairy cows to save protein feed production. Results: Low protein amino acid balanced diet had no significant effect on rumen fermentation parameters such as rumen pH, NH&lt;sub&gt;3&lt;/sub&gt;-N and TVFA. At the genus level, the relative abundance of &lt;em&gt;Ruminococcus&lt;/em&gt; in LP group [LP, low-protein diet with 16.0 % of crude protein +50 g/cow/d rumen-protected lysine (RP-Lys) + 30 g/cow/d rumen-protected methionine (RP-Met)] was significantly decreased. In the Linear discriminant analysis Effect Size (LEfSe) analysis, a total of 54 microorganisms with significant differences in relative abundance between groups were screened from phylum to genus. The abundance of 35 species such as &lt;em&gt;Ruminococcus&lt;/em&gt; and &lt;em&gt;Ruminobacillus&lt;/em&gt; in HP group (HP = high-protein group, regular diet with 17.5 % of crude protein) was significantly higher than that in LP group, while the abundance of 19 species such as &lt;em&gt;Vibrio butyrate&lt;/em&gt; and &lt;em&gt;Helicobacter&lt;/em&gt; was significantly lower than that in LP group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). The cows in LP group had higher dry matter intake (DMI) than the HP group after 90d. The dietary nitrogen intake in LP group was markedly lower than that in HP group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). Compared to HP group, the total nitrogen excretion was significantly lower in LP group (&lt;em&gt;P&lt;/em&gt; &lt; 0.01). The LP group treatment increased the nitrogen utilization efficiency (&lt;em&gt;P&lt;/em&gt; &lt; 0.05) when compared with HP group. Through metabolomics analysis, 620 differential metabolites were screened out from fecal samples, of which 329 were significantly up-regulated, such as uracil, uridine and uric acid, and 291 were significantly down-regulated, such as cholesterol, xanthine and &lt;em&gt;N&lt;/em&gt;-acetyl serotonin. 502 differential metabolites were screened out from urine samples, of which 268 were significantly up-regulated, such as creatine, tryptamine and phenylacetylglycine (PAGly), and 234 were significantly down-regulated, such as phenol, L-proline (L-Pro) and L-tyrosine (L-Tyr). KEGG pathway enrichment analysis showed that LP group had significant differences in protein digestion and absorption, nucleotide metabolism, amino acid biosynthesis and metabolism. Conclusion: Adding protective rumen lysine and methionine to low-","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105901"},"PeriodicalIF":1.8,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of dysregulated miRNA transcriptomic profile in canine hepatocellular carcinoma and comparison to human HCC","authors":"Saki Hashimoto ,&nbsp;M.D. Nazmul Hasan ,&nbsp;Mohammad Arif ,&nbsp;Nobuhiro Nozaki ,&nbsp;Al Asmaul Husna ,&nbsp;Yu Furusawa ,&nbsp;Takeshi Sogawa ,&nbsp;Kaori Takahashi ,&nbsp;Tomohide Kuramoto ,&nbsp;Aki Noguchi ,&nbsp;Masashi Takahashi ,&nbsp;Md Mahfuzur Rahman ,&nbsp;Naoki Miura","doi":"10.1016/j.rvsc.2025.105903","DOIUrl":"10.1016/j.rvsc.2025.105903","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) is the most common liver tumor in dogs. MicroRNA (miRNA) dysregulation and its clinical significance have been reported in dog and human cancers, including HCC. However, determining the whole miRNA transcriptomic profile is essential for diagnosis and therapeutics. Here, we determined the miRNA transcriptomic profile of canine HCC. First, miRNA expression from three control liver tissues and three canine HCC patients was analyzed via small RNA next-generation sequencing (NGS). After analyzing the sequencing reads, differential miRNA (DEmiRNA) expression was further confirmed in the clinical samples and three HCC cell lines via qRT-PCR. The functional pathways of the DEmiRNAs were analyzed from an experimentally validated human miRNA-target database. Our study revealed 20 upregulated and nine downregulated miRNAs. Among them, 10 miRNAs were further validated by qRT-PCR. qRT-PCR and NGS revealed similar miRNA expression patterns. Furthermore, we investigated the signaling pathways based on the KEGG pathway analysis and performed a functional analysis of the dysregulated miRNAs in canine HCC. The upregulated miRNAs mainly play a role in the p53 and cell cycle pathways and function in DNA damage repair and differentiation. In contrast, the downregulated miRNAs are involved in cancer and cell cycle pathways and mainly function in cell differentiation. Further, these miRNAs may be investigated in light of miRNA-based diagnosis and therapies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105903"},"PeriodicalIF":1.8,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative use of conventional and DNA metabarcoding methods reveals high diversity, prevalence and intensity of gastrointestinal parasites in pigs from Victoria, Australia","authors":"Ghazanfar Abbas ,&nbsp;Yuet On Lee ,&nbsp;Hannah Mitchell ,&nbsp;Abdul Ghafar ,&nbsp;Mark A. Stevenson ,&nbsp;Charles G. Gauci ,&nbsp;Leah G. Starick ,&nbsp;Regina M. Fogarty ,&nbsp;Bernie Gleeson ,&nbsp;Dianne E. Phillips ,&nbsp;Tao Wang ,&nbsp;Robin B. Gasser ,&nbsp;Abdul Jabbar","doi":"10.1016/j.rvsc.2025.105902","DOIUrl":"10.1016/j.rvsc.2025.105902","url":null,"abstract":"<div><div>This cross-sectional study provides the first integrated parasitological and molecular survey of gastrointestinal parasites in pigs from 69 commercial and backyard farms across Victoria, Australia. In total, 1222 faecal samples were tested using the modified McMaster technique to determine faecal egg and oocyst counts. Samples test-positive for strongylid nematode stages were characterised further using next-generation sequencing (NGS) targeting the second internal transcribed spacer (ITS-2) of ribosomal DNA as a species-specific genetic marker. Based on faecal egg/oocyst count, 39.1 % of farms tested positive for at least one parasite taxon. Coccidia were most prevalent (9.5 %) in samples, followed by <em>Ascaris suum</em> (5.2 %), <em>Trichuris suis</em> (4.5 %) and strongylid nematodes (1.8 %). Infection intensity was highest for coccidia, with notable burdens also for <em>Ascaris suum</em> and strongylid in individual samples. Eggs of <em>Metastrongylus</em> spp. were detected for the first time in Australian domestic pigs on an outdoor farm. Prevalence patterns varied substantially according to the production system and pig age group: coccidia dominated in mature breeders (17.8 %) and piglets (15.4 %) in backyard settings (33.7 %), whereas <em>Ascaris</em> peaked in mature breeders (9.1 %) and growers/finishers (up to 5 %) in semi-intensive systems (21.7 %). Strongylid eggs were most frequently detected in gilts and mature breeders from backyard and extensive systems. Notably, NGS revealed a diverse community of strongylid nematodes, including <em>Oesophagostomum dentatum, O. quadrispinulatum</em> and <em>Trichostrongylus axei</em> and <em>Metastrongylus apri</em> and <em>M. salmi</em>. This is the first molecular detection of <em>Metastrongylus</em> species in domestic pigs in Australia. These findings uncover a considerable and underappreciated parasitic burden in pig populations across multiple production systems in Victoria.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105902"},"PeriodicalIF":1.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}