Research in veterinary science最新文献

{"title":"Staphylococcus haemolyticus isolated from the Elaphurus davidianus: Pathogenicity and antimicrobial susceptibility profile","authors":"Yongcheng Meng ,&nbsp;Qingyan Han ,&nbsp;Mengling Han ,&nbsp;Zhenpeng Li ,&nbsp;Jie Wang ,&nbsp;Sihan Zuo ,&nbsp;Zhen Huang ,&nbsp;Qian Mu ,&nbsp;Yongli Hua ,&nbsp;Wanling Yao ,&nbsp;Wangdong Zhang","doi":"10.1016/j.rvsc.2026.106116","DOIUrl":"10.1016/j.rvsc.2026.106116","url":null,"abstract":"<div><div><em>Staphylococcus haemolyticus</em> is an important zoonotic pathogen. The objective of this study was to investigate the pathogenicity of a <em>Staphylococcus haemolyticus</em> isolated from the blood of <em>Elaphurus davidianus</em> and evaluate its potential risks to public health. A combination of methods including 16S rRNA sequencing, PCR, antimicrobial susceptibility testing and mouse challenge assay was employed to systematically investigate its phylogenetic relationships, growth characteristics, virulence and antimicrobial resistance genes, LD₅₀, and antimicrobial resistance profile. The results indicated that the strain was identified as <em>Staphylococcus haemolyticus.</em> The LD₅₀ of the strain in mice was determined to be 6.4 × 10⁸ CFU/ml, and the strain caused significant pathological lesions in the heart, lung, spleen, kidney and liver of the mice. In addition, the strain harbored 8 virulence genes, including <em>Lipase</em>, <em>Polyglutamic acid capsule</em>, <em>Uge</em>, <em>WbtP</em>, <em>Thermonuclease</em>, <em>Type VII secretion system</em>, <em>Autolysin,</em> and <em>Cytolysin</em>. Meanwhile, it exhibited resistance to 18 antibiotics, such as oxacillin, ampicillin, benzylpenicillin, levofloxacin, ceftazidime, streptomycin, imipenem, minocycline, tetracycline, doxycycline, azithromycin, erythromycin, clindamycin, lincomycin, vancomycin, chloramphenicol, florfenicol, and cotrimoxazole, and harbored 12 antimicrobial resistance genes, including <em>msrA</em>, <em>mphC</em>, <em>PC1</em>, <em>AAC(6′)-Ie-APH(2″)-Ia, vanY</em> gene in <em>vanB</em> cluster, <em>vanY</em> gene in <em>vanM</em> cluster, <em>APH(3′)-IIIa, vanT</em> gene in <em>vanG</em> cluster, <em>sepA</em>, <em>norC</em>, <em>sdrM</em>, and <em>dfrG</em>. Collectively, these findings indicated that the strain possessed strong virulence and exhibited resistance to multiple antibiotics. This suggested that it posed considerable potential risks to public health. This study provides a scientific basis for further investigation into the pathogenic mechanisms, antimicrobial resistance mechanisms, and prevention and control strategies of this strain.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106116"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147322110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinary perspectives on biosecurity measures in small-scale farming systems across European countries: Results from a cross-national survey","authors":"F. Duarte ,&nbsp;J. Prodanov-Radulović ,&nbsp;B. Mehmedi ,&nbsp;A. Piccirillo ,&nbsp;M. Stukelj ,&nbsp;E. Kldiashvili ,&nbsp;V. Nedosekov ,&nbsp;B. Milovanovic ,&nbsp;N.D. Ayaz ,&nbsp;R. Yildiz ,&nbsp;S. Meling ,&nbsp;I. Toftaker ,&nbsp;A. Sirbu ,&nbsp;A. Dodovski ,&nbsp;A. Allepuz ,&nbsp;A. Zbikowski ,&nbsp;Xh. Koleci ,&nbsp;C. Berger ,&nbsp;J. Starič ,&nbsp;M.E. Filippitzi ,&nbsp;I.M.G.A. Santman-Berends","doi":"10.1016/j.rvsc.2026.106089","DOIUrl":"10.1016/j.rvsc.2026.106089","url":null,"abstract":"<div><div>Maintaining sufficient levels of biosecurity at the farm level is crucial to prevent the introduction and spread of pathogens. However, data on biosecurity awareness and implementation in small-scale or backyard (hereafter referred to as small-scale) holdings across Europe are lacking. While the risk posed by these farms is generally regarded as limited, they represent a diverse group of farmers and do play a role in disease introduction and spread. The aim of this study was to explore veterinarians' perspectives on small-scale farming in Europe, including the frequency of farm visits, the definition of small-scale farming, the current status of biosecurity, and the most important measures to prevent pathogen introduction and spread. A multidisciplinary team developed a comprehensive survey to collect veterinarians' perspectives on biosecurity in small-scale farms. The survey was translated into 16 languages, digitalised, and distributed online between July and December 2024 by participants of COST Action BETTER. Data were analysed descriptively, and clusters of veterinarians were distinguished using multiple correspondence analysis. In total, 346 veterinarians from 23 countries participated in the survey. There is no standard definition of small-scale farming throughout Europe. According to the participating veterinarians, a small-scale farm was defined as having a median number of fewer than 20 cattle, 30 small ruminants, 13 pigs, or 99 heads of poultry. Three clusters of veterinarians were identified based on geographical location, field experience, biosecurity training, and perceived biosecurity awareness within the small-scale farming community. Biosecurity measures already applied in small-scale farming included fencing, carcass management, and biosecurity measures related to purchasing and feeding practices. According to the participating veterinarians, the most important biosecurity measures for small-scale farms to prevent pathogen introduction and spread included factors related to the introduction of animals, such as quarantine facilities, testing for health status, and purchasing animals from farms with a high health status. Besides factors related to the introduction of animals, hygiene measures and fencing were also frequently indicated as the most important biosecurity measures. Nevertheless, significant differences were found between the clusters regarding the importance of specific biosecurity measures. This study provides valuable insights into the perceived awareness, implementation, and desired biosecurity measures in European small-scale farming and underscores the importance of integrating small farms into “One Health” strategies, given their potential role in the spread of infectious diseases.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106089"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One health strategies for zoonosis prevention in developing countries: A systematic review (2015–2025)","authors":"Kunta Adnan Sahiman ,&nbsp;Siti Azizah ,&nbsp;Kuswati Kuswati ,&nbsp;Andi Moh. Iekram","doi":"10.1016/j.rvsc.2026.106091","DOIUrl":"10.1016/j.rvsc.2026.106091","url":null,"abstract":"<div><h3>Background</h3><div>Despite growing global investment in One Health initiatives, limited systematic synthesis exists examining veterinary authority strategies and their performance outcomes in developing countries, creating a knowledge gap for evidence-based policy formulation.</div></div><div><h3>Objective</h3><div>To develop a comprehensive taxonomy of One Health zoonosis prevention strategies implemented by veterinary authorities in developing countries and establish quantitative effectiveness patterns across resource-constrained settings.</div></div><div><h3>Methods</h3><div>We conducted a systematic review following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines, searching Scopus, Web of Science, and PubMed for peer-reviewed studies published between January 2015 and July 2025. Two reviewers independently screened 126 records after deduplication with inter-rater agreement (κ = 0.82). We employed mixed-methods synthesis integrating thematic analysis and quantitative effectiveness scoring across 42 included studies.</div></div><div><h3>Results</h3><div>From 42 studies spanning 18 countries, we identified 14 distinct One Health strategies organized into three dominant modalities: intersectoral coordination mechanisms (35.7%), surveillance system enhancement (28.6%), and integrated service delivery (21.4%). Integrated approaches consistently demonstrated superior effectiveness scores (77–90, 95% CI: 73–94) compared to single-intervention strategies (25–95, 95% CI: 18–88). Mobile surveillance systems achieved 14-fold reporting increases (RR = 14.0, 95% CI: 13.8–14.2), while integrated surveillance systems demonstrated improved epidemiological understanding across human-animal interfaces.</div></div><div><h3>Conclusions</h3><div>Implementation quality and strategic integration represent more critical determinants of success than intervention type or resource intensity alone. Sub-optimal policy implementation and poor inter-sectoral coordination were consistently identified as barriers to achieving desired outcomes. These findings provide evidence-based guidance for optimizing limited resources in developing countries.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106091"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Amitraz treatments do not increase the frequency of mutations in the β2-adrenergic octopamine receptor in Varroa destructor: a field study in Central Spain","authors":"Antonio Pérez-Pérez ,&nbsp;Carolina Bartolomé ,&nbsp;Soledad Sagastume ,&nbsp;Aránzazu Meana ,&nbsp;Raquel Martín-Hernández ,&nbsp;Xulio Maside ,&nbsp;Mariano Higes","doi":"10.1016/j.rvsc.2026.106101","DOIUrl":"10.1016/j.rvsc.2026.106101","url":null,"abstract":"<div><div>The ectoparasitic mite <em>Varroa destructor</em> is a major threat to <em>Apis mellifera</em> colonies. Beekeepers use acaricides such as amitraz for control, but resistance has been reported, possibly linked to mutations in the <em>Octβ2R</em> gene. This study examined the frequency of these mutations in <em>Varroa</em> populations from two management systems: organic (oxalic acid treatment) and conventional (amitraz treatment). DNA was extracted individually from 83 mites collected from 14 apiaries, and a 1064 bp fragment of <em>Octβ2R</em> was amplified and sequenced. The F290L-related mutation appeared at a high frequency (89%), with no significant differences between treatments. No other reported mutations were found. The widespread homozygosity of F290L and lack of treatment-related variation suggest that this mutation existed before amitraz use, reflecting historical genetic variation rather than recent selection. Further research is needed to clarify its role in amitraz resistance.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106101"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five-year evidence synthesis of African swine fever in domestic pigs in Southeast Asia: A systematic review and meta-analysis","authors":"Lintang Winantya Firdausy ,&nbsp;Faisal Fikri ,&nbsp;Salipudin Tasil Maslamama ,&nbsp;Muhammad Thohawi Elziyad Purnama","doi":"10.1016/j.rvsc.2026.106088","DOIUrl":"10.1016/j.rvsc.2026.106088","url":null,"abstract":"<div><div>African swine fever (ASF) continues to threaten pig production systems across Southeast Asia, yet regional epidemiological patterns remain fragmented. This systematic review and meta-analysis synthesized prevalence data to provide a consolidated assessment of ASF burden, spatial distribution, and temporal trends from 2019 to 2023. Following PRISMA 2020 guidelines, a comprehensive literature search was conducted across eight major databases and supplementary sources, with protocol registration in the Open Science Framework. Four reviewers independently screened studies, and eligible publications underwent quality appraisal using the Joanna Briggs Institute (JBI) checklist. Data extraction encompassed prevalence estimates, diagnostic methods, sample types, and geographic information, which were subsequently mapped using QGIS. Meta-analyses, subgroup analyses, meta-regression modelling, and Egger's test were performed in R Studio using the “meta” and “metafor” packages. Twelve studies met inclusion criteria, representing seven Southeast Asian countries. The random-effects model yielded an overall pooled prevalence of 29.14% (95% CI: 20.56–37.72), with substantial heterogeneity (<em>I</em>^<em>2</em> = 100%; <em>τ</em>^<em>2</em> = 0.1012). Subgroup analyses demonstrated marked variations by study period, diagnostic method, sample type, and country, with the highest prevalence estimates reported in Vietnam and Lao PDR. Meta-regression identified a significant decline in prevalence over time (<em>p</em> &lt; 0.001). Spatial mapping revealed pronounced regional burden prevalence, particularly in the Lao PDR and southern Vietnam. Funnel plot asymmetry and regression testing suggested the presence of small-study effects. These findings underscore the persistent and heterogenous nature of ASF across Southeast Asia, emphasizing the need for harmonized surveillance, strengthened biosecurity in smallholder systems, and enhanced diagnostic capacity.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106088"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of injection site on the pharmacokinetics of danofloxacin in Pekin ducks (Anas platyrhynchos domestica)","authors":"Kamil Uney ,&nbsp;Orhan Corum ,&nbsp;Duygu Durna Corum ,&nbsp;Devran Coskun ,&nbsp;Pedro Marin ,&nbsp;Mario Giorgi ,&nbsp;Amnart Poapolathep ,&nbsp;Saranya Poapolathep ,&nbsp;Tugce Karaahmetli ,&nbsp;Enver Yazar ,&nbsp;Muammer Elmas","doi":"10.1016/j.rvsc.2026.106122","DOIUrl":"10.1016/j.rvsc.2026.106122","url":null,"abstract":"<div><div>The purpose of this study was to investigate the influence of injection site on danofloxacin pharmacokinetics in Pekin ducks. Thirty-two male Peking ducks were divided into 4 equal groups as intravenous (IV), pectoral muscles (IMP), thigh muscles (IMT) and subcutaneous (SC). Danofloxacin was administered to all groups at a dose of 10 mg/kg. Plasma concentrations of danofloxacin were quantified by HPLC-UV. The volume of distribution at steady state and total clearance values of danofloxacin after IV injections in ducks were 4.43 L/kg and 0.42 L/h/kg, respectively. The terminal elimination half-life (t_1/2ʎz), area under the concentration-versus time curve (AUC)_0-last, and peak plasma concentration (C_max) were similar after SC and IMP injections of danofloxacin. However, t_1/2ʎz was shorter and AUC_0-last and C_max were lower in the IMT group compared to the IMP group. The bioavailability was lower in the IMT group than in the IMP and SC groups. Danofloxacin exhibited similar pharmacokinetic profiles after SC and IMP injection into Pekin ducks. In the IMT group, pharmacokinetics altered significantly, resulting in reduced body exposure and residence time of danofloxacin. Optimizing the injection site to subcutaneous or pectoral muscle routes may improve danofloxacin's therapeutic efficacy in Pekin ducks, while caution should be applied with thigh muscle injections due to reduced bioavailability and systemic exposure.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106122"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147309596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in expression of growth, immune activity and adaptation related genes in heat stressed dorper and second cross lambs","authors":"Aleena Joy ,&nbsp;Swagat Mohapatra ,&nbsp;Frank R. Dunshea ,&nbsp;Brian J. Leury ,&nbsp;Kristy DiGiacomo ,&nbsp;Minghao H. Zhang ,&nbsp;Archana Abhijith ,&nbsp;Richard Osei- Amponsah ,&nbsp;Iain J. Clarke ,&nbsp;Surinder Singh Chauhan","doi":"10.1016/j.rvsc.2026.106087","DOIUrl":"10.1016/j.rvsc.2026.106087","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106087"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating multi-omics to characterize the dynamics of rumen microorganisms and metabolites in Angus cattle at different growth stages","authors":"Lin Xue ,&nbsp;Wei Zhao ,&nbsp;Chuanchuan Wang ,&nbsp;Yun Ma ,&nbsp;Jinli Tian ,&nbsp;Lijuan Yang ,&nbsp;Lina Ma ,&nbsp;Qiufei Jiang ,&nbsp;Yafei Chen ,&nbsp;Xiaohua Tian ,&nbsp;Xiaoyun Ji ,&nbsp;Juan Zhang ,&nbsp;Yaling Gu","doi":"10.1016/j.rvsc.2026.106092","DOIUrl":"10.1016/j.rvsc.2026.106092","url":null,"abstract":"<div><div>The development of the bovine rumen microbiome is crucial for growth, yet the dynamic interactions between the microbiome and metabolome during key growth stages remain poorly understood. This study aims to integrate metagenomics and metabolomics approaches to decipher the stage-specific patterns of rumen microbial community and metabolite changes in castrated Angus cattle at three critical growth stages (6, 12, and 18 months of age), and to elucidate their associations with host growth performance. We collected rumen fluid samples from 24 Angus steers (8 per age group) reared under standardized conditions and performed metagenomic and non-targeted metabolomic analyses. Integrated analysis revealed distinct rumen ecosystem succession patterns: multiple species represented by <em>Prevotella_sp._ne3005</em> dominated at 6 months, <em>Fibrobacter_succinogenes</em> showed significantly increased abundance at 12 months, and <em>Methanobrevibacter_millerae</em> exhibited the most pronounced enrichment at 18 months. Concurrently, key metabolites 12,13-Dihydroxyoleic Acid, Delta-12-Pgj2, and Cortisol exhibited a significant positive correlation with age. Further Pearson correlation analysis revealed strong correlations between the 18-month-enriched characteristic microorganism <em>Methanobrevibacter_millerae</em> and key metabolites (12,13-Dihydroxyoleic Acid, Delta-12-Pgj2, and Cortisol) as well as higher body weight. This study delineates a dynamic map of synergistic interactions between the rumen microbiome and metabolome, confirming their close association with host growth performance. This work provides a systematic multi-omics framework for understanding rumen development in ruminants and identifies potential targets for optimizing beef cattle production performance through microbial or metabolic interventions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106092"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146113815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial and temporal network analysis of 2021 swine trading movements in the Philippines using government-issued permits","authors":"Toni Rose M. Barroga ,&nbsp;Michael W. Sanderson ,&nbsp;Helene Avocat ,&nbsp;Reildrin G. Morales","doi":"10.1016/j.rvsc.2026.106100","DOIUrl":"10.1016/j.rvsc.2026.106100","url":null,"abstract":"<div><div>Animal movement plays a critical role in the spread of infectious diseases. Social network analysis can be used to understand animal movement data (AMD) by analyzing trading dynamics and identifying key nodes that are vulnerable to disease spread. This study used swine local shipping permits (LSP) in 2021 from the Philippines' AMD platform. Towns were considered nodes and arcs were defined as shipments, weighted by the number of shipments or pigs per shipment. The year was divided into four quarters to evaluate seasonal trends. R (igraph package) and ArcGISPro were used to analyze and map the network. Results showed a network structure of 685 towns from 37,138 LSPs representing 2,178,536 pigs shipped from 311 towns and delivered to 516 towns with a median travel distance of 102.35 km. Network-level density was 0.06%, transitivity 7.9%, assortativity −0.277, reciprocity 0.014, and average path length 3.73. The mean number of shipments and pigs shipped indicated seasonal variation; October–December was highest (55.5, 3167) while January–March was lowest: (29.5, 1861.5). The Walktrap algorithm identified six large trade communities, involving 21 to 282 towns. Towns with breeder and fattener farms showed high out-degree while high in-degree were associated with metropolitan cities and slaughterhouses. Seasonal trends are consistent with cultural practices with fewer shipments during January–March (Lent) and more shipments during October–December (Christmas). Network-level metrics suggest weak clustering, and one-sided trade relationships. Knowledge of the swine trading patterns is critical in controlling ASF to support risk-based surveillance and proper budgetary resource allocation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106100"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The TND-RTM protocol for controlling sporotrichosis in a free-roaming cat colony in southern Brazil","authors":"Brunna Gabriela Gonçalves de Oliveira Ferreira ,&nbsp;Vitor Gonçalves Teixeira ,&nbsp;Carla Forte Maiolino Molento ,&nbsp;Danielle Ferreira de Magalhães Soares ,&nbsp;Renato Silva de Souza ,&nbsp;Rita de Cassia Maria Garcia","doi":"10.1016/j.rvsc.2026.106108","DOIUrl":"10.1016/j.rvsc.2026.106108","url":null,"abstract":"<div><h3>Objective</h3><div>To describe the outcomes of the Trap, Neuter, Diagnosis, Return, Treatment, and Monitoring (TND-RTM) protocol applied to a free-roaming cat colony in a sporotrichosis-endemic area in southern Brazil.</div></div><div><h3>Methods</h3><div>Cats were captured and neutered, clinically assessed for sporotrichosis, and managed according to predefined colony-level therapeutic criteria, whereby the colony was classified as sporotrichosis-positive when at least one individual was affected. All cats within a positive colony were included in antifungal management, including isolation of clinically affected cats and preventive treatment of exposed animals, with continuous visual monitoring.</div></div><div><h3>Results</h3><div>At baseline, 52.4% of cats (11/21) presented clinical lesions compatible with sporotrichosis. Nineteen cats were captured and clinically evaluated, while two were monitored through visual assessment. Due to demographic changes during follow-up, 11.1% of cats remaining in the colony at 12 months (2/18) still exhibited active lesions. By the end of the 18-month follow-up period, no cats in the colony presented active sporotrichosis lesions (0/18).</div></div><div><h3>Conclusions</h3><div>The application of the TND-RTM protocol in a single free-roaming cat colony was associated with the resolution of clinical sporotrichosis cases and with improvements in animal welfare at the colony level. This descriptive case study highlights both the potential and the operational challenges of a colony-based approach integrating population management with infectious disease control in endemic urban settings.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106108"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}