Research in veterinary science最新文献

{"title":"Virulence factors and strain similarity of Staphylococcus aureus from a dairy farm demonstrate an increased risk of environmental transmission","authors":"Angela Perdomo,&nbsp;Juan Sebastian Solano-Gutierrez,&nbsp;Jonathan Thompson,&nbsp;Alexandra Calle","doi":"10.1016/j.rvsc.2025.105728","DOIUrl":"10.1016/j.rvsc.2025.105728","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is a highly adaptable microorganism that harbors virulence factors driving its pathogenicity, affecting animals and humans. Forty <em>S. aureus</em> isolates were collected from a dairy farm operation and were subjected to several phenomics, genomics, and proteomics tests, including antimicrobial susceptibility, biofilm evaluation, strain typing for isolate-relatedness determination, and enterotoxin production. Further characterization involved whole genome sequencing (WGS) and protein detection using mass spectrometry on a subset of isolates (enterotoxin-positive and enterotoxin-negative). All isolates were susceptible to the tested antibiotics except for Gatifloxacin (GAT), for which 100 % displayed intermediate resistance. All isolates produced biofilms, with a noticeable surge at 48 h compared to 24 h (<em>p</em> &lt; 0.001). Additionally, 10 % of the isolates (<em>n</em> = 4) produced enterotoxins associated with human infections. Strain typing via infrared biotyping (IRBT) categorized the 40 isolates into six distinctive groups, indicating close relationships among <em>S. aureus</em> recovered from different samples, demonstrating a movement of the organism within the farm. Genomic characterization of enterotoxin-positive and enterotoxin-negative isolates identified two sequencing types, ST151 and ST351, accompanied by spa types t529 and t9001. Genomics and proteomics analyses revealed various virulence factors, encompassing cytolytic toxins, immune evasion mechanisms, extracellular matrix (ECM)—binding proteins, proteases, enterotoxins, and chromosomal point mutations. In addition to virulence factors, this study shows how this bacterium moves around the farm, potentially becoming an environmental pathogen, increasing the risk of transmission and infection for humans and animals.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105728"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nationwide serological survey and risk factors for Toxoplasma gondii and Neospora caninum infections in alpacas (Vicugna pacos) and llamas (Lama glama) in Italy","authors":"Elisa Castaldo ,&nbsp;Walter Basso ,&nbsp;Gastón Moré ,&nbsp;Alessia Ciaramelli ,&nbsp;Nicola D'Alessio ,&nbsp;Michele Capasso ,&nbsp;Giovanni Sgroi ,&nbsp;Sara Tonon ,&nbsp;Farwa Humak ,&nbsp;Alessia Gazzonis ,&nbsp;Gereon Schares ,&nbsp;Vincenzo Veneziano","doi":"10.1016/j.rvsc.2025.105729","DOIUrl":"10.1016/j.rvsc.2025.105729","url":null,"abstract":"<div><div><em>Toxoplasma gondii</em> and <em>Neospora caninum</em> are worldwide distributed protozoa, recognized as major causes of economic losses in livestock production due to reproductive failure. Camelids can also serve as intermediate hosts for these protozoa. Research on these parasites in South American Camelids (SAC) in Europe is scarce. Therefore, this study aimed to estimate the seroprevalence and distribution of <em>T. gondii</em> and <em>N. caninum</em> infections in SAC in Italy and investigate risk factors for infection applying a structured online questionnaire filled in by owners. A total of 506 SAC sera (486 alpacas, <em>Vicugna pacos,</em> and 20 llamas, <em>Lama glama</em>) were tested to detect antibodies against <em>T. gondii</em> and <em>N. caninum</em> using enzyme-linked immunosorbent assays (ELISA), and results were confirmed with immunoblot. Seroprevalences of 34.8 % (176/506) for <em>T. gondii,</em> with 33.1 % (161/486) for alpacas and 75.0 % (15/20) for llamas, and 5.7 % (29/506) for <em>N. caninum,</em> with 5.6 % (27/486) for alpacas and 10.0 % (2/20) for llamas were found. Simultaneous presence of antibodies against <em>T. gondii</em> and <em>N. caninum</em> was detected in 3.6 % (18/506) of the samples. The variables older age and llama species were identified as risk factors (<em>p</em> &lt; 0.05) for <em>T. gondii</em>, while the presence of dogs and fertility disorders were associated with <em>N. caninum</em> seropositivity. This study provides the first large-scale serological evidence of <em>T. gondii</em> and <em>N. caninum</em> infections in alpacas and llamas in Italy, highlighting their widespread distribution and contributing valuable data to the national epidemiological scenario. Further studies are needed to evaluate the role of these parasites as cause of abortion.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105729"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre and post fibrate supplementation lipoprotein electrophoresis in hyperlipemic dogs with biliary tree disease","authors":"Eleonora Gori ,&nbsp;Saverio Paltrinieri ,&nbsp;Verena Habermaass ,&nbsp;Ilaria Lippi ,&nbsp;Veronica Marchetti","doi":"10.1016/j.rvsc.2025.105727","DOIUrl":"10.1016/j.rvsc.2025.105727","url":null,"abstract":"<div><div>Hyperlipemia is commonly observed in dogs with biliary tree disease, where cholestasis can both cause and result of hyperlipidemia. Fibrate therapy effectively reduce lipemia in dogs with both primary and secondary hyperlipidemia. This study aimed to investigate whether fenofibrate therapy could alter lipoprotein patterns in hyperlipemic cholestatic dogs. Retrospective cohort study on left-over frozen-stored (−80 °C) serum samples of 25 client-owned dogs with chronic biliary disease with hyperlipidemia (hypercholesterolemia and/or hypertriglyceridemia) that underwent fenofibrate therapy at 4–10 mg/kg once daily (T0) and had a re-check after 4–6 weeks (T1). To be included, dogs with hypercholesterolemia (&gt;280 mg/dL) and/or hypertriglyceridemia (&gt;90 mg/dL) had to present a concurrent increase of two or more between alkaline phosphatase (ALP) &gt;250 U/L, gamma-glutamyl transferase (GGT) &gt;11 U/L and total bilirubin &gt;0.3 mg/dL and abdominal ultrasound alterations suggestive for a chronic biliary tree disease. Pre- and post-treatment serum samples were analysed using lipoprotein electrophoresis. Results showed significant reductions in serum cholesterol (median 293 mg/dL vs. 368 mg/dL) and triglycerides (median 70 mg/dL vs. 181 mg/dL). Lipoprotein analysis revealed a significant increase in HDL percentage (from 51 % to 62.9 %) and reductions in VLDL (from 33.2 % to 18 %) and chylomicrons (from 3.2 % to 1.7 %). These results suggest that fenofibrate is effective in reducing lipemia and improving lipid profiles in cholestatic dogs, helping to limit hepatic injury potentially associated with lipid accumulation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105727"},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and risk factors of infectious diseases in smallholder pig farms in Vietnam","authors":"Yin Li ,&nbsp;Ninh Thi Huyen ,&nbsp;Ngo Thi Kim Cuc ,&nbsp;Nguyen Cong Dinh ,&nbsp;Tran Trung Thong ,&nbsp;Le Thu Ha ,&nbsp;Nguyen Pham Trung Nguyen ,&nbsp;Marta Hernandez-Jover ,&nbsp;Jennifer Kelly ,&nbsp;Jennifer Manyweathers","doi":"10.1016/j.rvsc.2025.105726","DOIUrl":"10.1016/j.rvsc.2025.105726","url":null,"abstract":"<div><div>Biosecurity is critical for a healthy pig herd. This study aims at understanding incidence risk and risk factors of any farmer reported disease infection and African swine fever outbreaks on Vietnamese small-scale pig farms. Using a cross-sectional survey in the Bac Giang, Ha Nam, and Hoa Binh Provinces, 160 randomly chosen small-scale pig farms provided information about husbandry, hygiene and biosecurity practices and occurrence of disease in the last six months, including African swine fever. Farm-level incidence risk of farmers reported “disease outbreaks” and “African swine fever outbreaks” were estimated, and risk factors were explored using logistic regression analyses. The crude incidence risk of any farmer reported disease was 51 % (95 % CI: 43–59 %) and the risk were 60 %, 52 % and 43 % in the Hoa Binh, Ha Nam, and Bac Giang Province, respectively. The relevant risk and protective factors were: Being close to another pig farm (odds ratio (OR) = 3.81, 95 % CI: 1.40–11.70); checking water contamination annually (OR = 0.25, 95 % CI: 0.07–0.77); and, cleaning and disinfecting the vehicles that enter the farm (OR = 0.37, 95 % CI: 0.16–0.83). The findings from this study will offer scientific evidence for establishing biosecurity guidelines in smallholder pig farms in Vietnam.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105726"},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus plantarum ameliorates stress caused by Pseudomonas aeruginosa infection in Nile tilapia","authors":"Lamiaa A. Okasha ,&nbsp;Walaa S. Tawfeek ,&nbsp;Shaimaa Elbaz ,&nbsp;Ahmed H. Sherif","doi":"10.1016/j.rvsc.2025.105721","DOIUrl":"10.1016/j.rvsc.2025.105721","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a highly pathogenic bacterium affecting the health of Nile tilapia and its production. Fifty moribund fish were collected from farms with high mortality. The causative agent was identified to be <em>P. aeruginosa</em>, and the median lethal dose (LD₅₀) was found to be 1.44 × 10⁵ CFU/mL at a water temperature of 26.5 ± 1.2 °C. To enhance fish immunity, one hundred and thirty-five healthy Nile tilapia were divided into three groups: control (basal diet), single dose of dietary <em>Lactobacillus plantarum</em> at dose of 1 × 10¹² CFU/kg feed, and double dose 2 × 10¹² CFU/kg feed, where supplemented fish showed significant enhancements of their growth performance, feed utilization, and innate immunity (up-regulating serum antibacterial activity, oxidative burst activity, and phagocytosis). Before infection, the gene expression of interleukin (<em>IL</em>)<em>-1β</em> and tumor necrosis factor (<em>TNF</em>)<em>-α</em> was slightly increased in the head kidney tissue of fish that received dietary <em>L. plantarum</em>, regardless of supplementation dose. Meanwhile, post <em>P. aeruginosa</em> infection, the expression of <em>IL-1β</em> and <em>TNF-α</em> was up-regulated significantly compared to the control. Post-infection, fish supplemented with dietary <em>L. plantarum</em> had a lower mortality rate (MR) (50 %) compared to the control (70 %), providing a 28.57 % relative level of protection (RLP). The Nile tilapia, experimentally infected with <em>P. aeruginosa</em>, showed gasping, decreased food apprehension, and loss of escape reflex.</div><div>The Nile tilapia, experimentally infected with <em>P. aeruginosa</em>, had some drawbacks, such as immunosuppressed status, abnormal behavior reflexes, and a high mortality rate that dietary <em>L. plantarum</em> could mitigate. Thus, Nile tilapia diet should be supplemented with <em>L. plantarum</em> at 1 × 10¹² or 2 × 10¹² CFU/kg feed.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105721"},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of hydrolysable and condensed tannin sources and levels on in vitro equine cecal fermentation","authors":"Lauren R. Wottlin ,&nbsp;Robin C. Anderson ,&nbsp;Raghavendra Bhatta ,&nbsp;Jessica Leatherwood ,&nbsp;Elena G. Olson ,&nbsp;Todd R. Callaway ,&nbsp;Steven C. Ricke","doi":"10.1016/j.rvsc.2025.105732","DOIUrl":"10.1016/j.rvsc.2025.105732","url":null,"abstract":"<div><h3>Objectives</h3><div>Environmental impacts of animal agriculture are under intense scrutiny, thus strategies to decrease greenhouse gas emissions and volatile nitrogen waste are valuable. As dietary inclusion of tannins has been shown to decrease methanogenesis and improve nitrogen retention in ruminants, this study was designed to determine the effects of graded levels of hydrolysable tannin (chestnut) and condensed tannin (mimosa) on equine <em>in vitro</em> cecal fermentation.</div></div><div><h3>Methods</h3><div>Two <em>in vitro</em> experiments were conducted, each with three replications per treatment. Treatments were 0.2 g alfalfa without or with 5, 10, 15, or 20 % (wt/vol) chestnut or mimosa tannin. Both tannins decreased (<em>P &lt;</em> 0.01) total gas and hydrogen accumulations by as much as 27 and 82 %, respectively, when compared to accumulations in nontreated controls (11 mL and 0.34 μmol/mL incubation fluid).</div></div><div><h3>Results</h3><div>Mimosa, but not chestnut, treatments decreased (<em>P &lt;</em> 0.01) methane accumulations, with decreases ranging from 4 to 83 % compared to controls (1.1 μmol/mL). Mimosa, but not chestnut, treatment decreased acetate accumulations by as much as 36 % compared to controls (14.8 μmol/mL) but neither tannin affected accumulations of butyrate (1.6 μmol/mL). Both tannin treatments increased (<em>P &lt;</em> 0.05) propionate accumulations by as much as 38 % compared to controls (5.7 μmol/mL). Ammonia accumulations were decreased by as much as 96 and 87 % by chestnut and mimosa treatments, respectively, compared to controls (0.24 μmol/mL).</div></div><div><h3>Conclusion</h3><div>These results indicate tannins may effectively reduce volatile nitrogen waste and methane emissions in equids and thus warrant further research to validate these results <em>in vivo</em>.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105732"},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Space–time clustering of rabies in equines in Brazil from 2006 to 2023","authors":"Valdir Vieira da Silva,&nbsp;Denny Parente de Sá Barreto Maia Leite,&nbsp;Lucilene Martins Trindade Gonçalves,&nbsp;Gustavo de Oliveira Alves Pinto,&nbsp;Pollyanne Raysa Fernandes de Oliveira,&nbsp;Rinaldo Aparecido Mota","doi":"10.1016/j.rvsc.2025.105724","DOIUrl":"10.1016/j.rvsc.2025.105724","url":null,"abstract":"<div><div>Rabies is a lethal zoonosis caused by the rabies virus (RABV), primarily transmitted by the common vampire bat (<em>Desmodus rotundus</em>). Brazil, home to the largest equine population in South America, faces ongoing challenges with equine rabies, which impacts both the economy and public health. This study aimed to identify spatial and temporal patterns of rabies in equines in Brazil between 2006 and 2023. Data from the Zoosanitary Information System (SIZ) of the Brazilian Ministry of Agriculture, Livestock, and Food Supply (MAPA) were used. The incidence risk (IR) was calculated for each region and Federative Unit. To identify spatial and temporal clusters, a spatiotemporal scan analysis was conducted using the Poisson model in SaTScan™. Temporal trends were analyzed using a segmented log-linear regression model in Joinpoint Regression. During the study period, 2170 cases of rabies in equines were reported in Brazil. The Southeast region accounted for 41.66 % of cases, followed by the Central-West (24.47 %). The Federative Units with the highest incidence risk were Espírito Santo (124.68/100,000 equines), São Paulo (69.97/100,000), and Rio de Janeiro (69.03/100,000). The retrospective spatiotemporal analysis identified four major clusters, notably in São Paulo, Mato Grosso, Espírito Santo, Rio de Janeiro, and Amazonas. The prospective analysis revealed active clusters in São Paulo, Espírito Santo, and Amazonas. Temporal trend analysis indicated a decline in equine rabies incidence in Brazil (APC: -3.9 %; CI: −6.2 to −1.5). The findings of this study provide valuable insights for the implementation of more effective preventive measures, directing epidemiological surveillance toward high-risk regions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105724"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microcomputed tomographic examination of the osseous structures of the canine carpus","authors":"L. Goldstein ,&nbsp;A.-C. Waselau ,&nbsp;Y. Zablotski ,&nbsp;A. Meyer-Lindenberg","doi":"10.1016/j.rvsc.2025.105725","DOIUrl":"10.1016/j.rvsc.2025.105725","url":null,"abstract":"<div><div>The surgical treatment of carpal joint injuries is associated with a high implant-based complication rate of up to 50 %. For this reason, the aim of the study was to create a database on the bony microarchitecture of the cancellous and cortical structures of the carpus. A total of 80 carpal joints from 20 medium-sized dogs and 20 toy breeds were examined and compared with each other using microcomputed tomography. The parameters bone volume (BV/TV), bone surface (BS/BV), trabecular thickness (Tb.Th), number of trabeculae (Tb.N), trabecular spacing (Tb.Sp), degree of anisotropy (DA) and connectivity density (Conn. D) were measured and compared. In addition, the cortical structure was classified using a three-staged scoring system. It was shown that all carpal bones have a cancellous structure, that differs clearly between the groups, without one group being mechanically superior. The evaluation implies, that the second carpal bone appears to be very stable. The formation of the cortex differs massively between the groups, with the toy breeds having only a very thin, partially interrupted bone lamella, whereas the medium-sized dogs have a normal cortex. Within the toy breed group inhomogeneous results were observed, whereby the values of the Chihuahuas deviated. This breed had significantly fewer (Tb.N) and thinner trabeculae (Tb.Th) with a greater trabecular separation (Tb.Sp), lower bone volume fraction (BV/TV) and higher bone surface (BS/BV). This indicates a decreased stability of the Chihuahua's carpal bones. The results of this study could potentially improve the development of new implants and thus reduce the complication rate.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105725"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies for combating FIPV infection: antiviral agents and vaccines","authors":"Fan Gao,&nbsp;Guilan Wen","doi":"10.1016/j.rvsc.2025.105709","DOIUrl":"10.1016/j.rvsc.2025.105709","url":null,"abstract":"<div><div>Feline infectious peritonitis virus (FIPV) is a deadly virus that causes feline infectious peritonitis (FIP) in cats. FIP is a biotype of feline coronavirus (FCoV). Currently, the prevention and treatment of FIPV is challenged by the absence of adequate clinical treatment drugs and vaccines that offer strong immune protection complicated. In this review, we analyse and explain how small-molecule inhibitors function to protect against viral infections. Additionally, we highlight the challenges and future possibilities in developing FIPV vaccines, which are crucial for enhancing FIPV treatment and creating viable vaccine solution strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105709"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does hypertriglyceridemia induce increased oxidative stress in obese dogs?","authors":"Yuri Lima Pereira ,&nbsp;Laura Elisa Raviolo ,&nbsp;Gabriela Fernanda Vitoretti Machado ,&nbsp;Danielle Venturini ,&nbsp;Karina Keller Marques da Costa Flaiban","doi":"10.1016/j.rvsc.2025.105723","DOIUrl":"10.1016/j.rvsc.2025.105723","url":null,"abstract":"<div><div>Obesity is considered the most common nutritional disorder in pets. It triggers a low-grade inflammatory process that can lead to increased oxidative stress and contribute to various diseases. The purpose of this study was to evaluate the systemic levels of oxidative stress biomarkers in dogs with overweight, obesity and ideal body condition score (BCS). Fifty-eight adult and senior dogs were selected and divided into three groups: overweight (n = 20), obese (n = 20), and ideal BCS (n = 18). Oxidative stress biomarkers were measured using lipid peroxidation products, by ferrous oxidation-xylenol orange (FOX), advanced oxidative protein products (AOPP), and total reactive antioxidant potential (TRAP), with C-reactive protein (CRP) concentration used as an inflammatory biomarker. Protein oxidation was observed in overweight and obese dogs, as evidenced by elevated AOPP levels (p = 0.004) and their ratio to antioxidant levels (AOPP/TRAP) through the prooxidant-antioxidant index (PAI) (p = 0.015) in obese dogs. In obese dogs with hypertriglyceridemia, lipid oxidation (p = 0.018) was observed in addition to protein oxidation (p &lt; 0.001). Furthermore, a positive correlation was observed between the triglyceride levels and AOPP (r = 0.716, p &lt; 0.001) and PAI (r = 0.749, p &lt; 0.001) concentrations. Finally, we concluded that obesity alone may not be sufficient to induce increased oxidative stress and that hypertriglyceridemia is correlated with protein and lipid oxidation in obese dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105723"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}