Research in veterinary science最新文献

{"title":"Interleukin-6 promotes the epithelial mesenchymal transition in canine tonsillar squamous cell carcinoma cells","authors":"Shunsuke Noguchi ,&nbsp;Ryo Shimonishi","doi":"10.1016/j.rvsc.2024.105487","DOIUrl":"10.1016/j.rvsc.2024.105487","url":null,"abstract":"<div><div>Canine oral squamous cell carcinoma (CoSCC) is often associated with suppurative inflammation. Metastasis of malignant tumors is one of the signs of major interest in oncology because it speaks of disease progression, where the involvement of interleukin-6 (IL-6) in cancer progression is so far unknown. Therefore, the aim of this study was the determination of the role of IL-6 in metastasis in CoSCC cells model through expression analysis of mRNA and protein using real-time PCR and western blotting and assessment of cell migration and invasion. The messenger RNA (mRNA) expression level of <em>IL-6</em> was elevated in CoSCC tissues, and the IL-6 receptor protein was expressed in CoSCC cell lines. Furthermore, <em>IL-6</em> levels were associated and showed negative correlation with survival time (rs = −0.92857) in dogs with tonsillar SCC. Recombinant canine IL-6 (rcIL-6) treatment promoted migration and invasion, in addition to increasing the viable cell number of the tonsillar SCC cell line (TSCCLN#6). Consistently, the protein expression of phosphorylated ERK1/2 and STAT3 and Fascin1 (FSCN1) was upregulated by treatment with rcIL-6 in a dose-dependent manner. Treatment with ERK or STAT3 inhibitors abolished the effects of rcIL-6, and the ERK inhibitor successfully downregulated the expression of FSCN1. In conclusion, IL-6 may be involved in tonsillar CoSCC invasion and metastasis through the activation of the mitogen-activated protein kinase and Janus tyrosine kinase/signal transducer and activator of transcription signaling.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105487"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transport-related respiratory pathogens in horses travelling long distances: A prospective study on arrival at the slaughterhouse","authors":"Barbara Padalino ,&nbsp;Francesco Pellegrini ,&nbsp;Martina Felici ,&nbsp;Martina Zappaterra ,&nbsp;Cristiana Catella ,&nbsp;Marco Cordisco ,&nbsp;Eleonora Lorusso ,&nbsp;Francesco Cirone ,&nbsp;Annamaria Pratelli","doi":"10.1016/j.rvsc.2024.105498","DOIUrl":"10.1016/j.rvsc.2024.105498","url":null,"abstract":"<div><div>Respiratory disorders (RDs) are a common transport-related welfare consequence in horses. This prospective study described the journeys of horses transported to two slaughterhouses in Italy, documented the prevalence of potentially RD-related pathogens, and identified possible predisposing factors. Data were recorded from arrival until 48 h after arrival and included the collection of Deep Nasopharyngeal Swabs (DNS), journey details and welfare assessment of horses (<em>n</em> = 155). PCR was used to quantify the presence of influenza A virus, reovirus, equine herpesvirus type 1 and 4, adenovirus, rhinovirus, astrovirus, equine coronavirus, <em>Pasteurellaceae</em>, <em>Streptococcus equi</em> sub. <em>e</em><em>qui</em> and sub. <em>z</em><em>ooepidemicus</em>. One horse each tested positive for reovirus, and rhinovirus and two horses tested positive for adenovirus. The prevalence of <em>Streptococcus equi</em> and <em>zooepidemicus</em> and <em>Pasteurellaceae</em> was 4 %, 19 % and 63 % respectively. <em>Streptococcaceae</em> and <em>Pasteurellaceae</em> were associated with sweating (<em>P</em> &lt; 0.001). In addition, <em>Streptococcaceae</em> was associated with abnormal demeanour (<em>P</em> = 0.003), nasal or ocular discharge (<em>P</em> &lt; 0.001) and higher compromised welfare after transport (P &lt; 0.001). The multivariable regression analysis showed that broken horses departing from Eastern Europe or France were more likely to test positive for <em>Streptococcaceae</em> than unbroken horses coming from Poland (model <em>P</em> &lt; 0.001), while broken horses subjected to short stops <em>en route</em> were more likely to test positive for <em>Pasteurellaceae</em> than unbroken horses on journeys without stops (model <em>P</em> &lt; 0.001). Our findings increase the understanding of predisposing factors for RDs and may be useful in the implementation of regulations to protect the welfare of horse during transport.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105498"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization of infectious bursal disease virus strains with distinct VP2 amino acid profiles emerging in Pakistan","authors":"Sanaullah Sajid ,&nbsp;Haseeb Ullah Sajid","doi":"10.1016/j.rvsc.2024.105509","DOIUrl":"10.1016/j.rvsc.2024.105509","url":null,"abstract":"<div><div>Infectious bursal disease (IBDV) poses a significant threat to the global poultry industry and causes major economic losses. This study presents the genetic profile of IBDV strains emerging in Pakistan, focusing on the VP2 amino acid profile. The effects of these changes on disease transmission, vaccine effectiveness, and overall chicken health are concerning. A meticulous analysis was carried out by isolating and clustering IBDV strains collected from Faisalabad district of Pakistan. The genetics of these viruses were evaluated by analyzing the VP2 gene, the main component of the virus. Importantly, our results reveal the existence of IBDV strains with unprecedented VP2 amino acid profiles, indicating genetic changes that may affect virulence and immunity. This study highlighted the changing IBDV landscape in Pakistan and underscores the importance of continued research and surveillance efforts. Understanding the genetic structure of these emerging diseases is essential for developing effective control strategies, including vaccine development and control. This also emphasized the need for international cooperation to reduce the spread of the new IBDV strains, which could significantly impact the poultry industry outside Pakistan.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105509"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of transdermal ketoprofen on surgical inflammation in dogs","authors":"H.G. Ravuri ,&nbsp;P. Sadowski ,&nbsp;N. Satake ,&nbsp;P.C. Mills","doi":"10.1016/j.rvsc.2024.105512","DOIUrl":"10.1016/j.rvsc.2024.105512","url":null,"abstract":"<div><div>Ketoprofen is a non-steroidal anti-inflammatory drug (NSAID) used to treat pain and inflammation in dogs. Despite having effective analgesic efficacy, prolonged oral administration has been associated with adverse effects. Transdermal delivery of ketoprofen has reduced the incidence of adverse effects in humans and could potentially be used in veterinary clinical medicine. A transdermal (TD) ketoprofen formulation was recently developed for use in dogs and this study aimed to determine the pharmacodynamic activity of this formulation using surgical castration as an acute inflammatory model. Twelve dogs were randomly assigned to either a Control group (<em>n</em> = 6) or a TD group (<em>n</em> = 6). All dogs were castrated using standard surgical protocols, administered with pre-medication, consisting of acepromazine (0.055 mg/kg) and methadone (0.5 mg/kg) intramuscularly (IM) 30 min prior to induction of general anaesthesia. All dogs were then anaesthetised by injecting alfaxalone (2 mg/kg IV) via a 20 G 3 cm catheter in the left cephalic vein and subsequently maintained using isoflurane. Along with that TD group dogs also received TD ketoprofen (10 mg/kg) 1 h before pre-medication. Bloods were collected at 0 – hour (pre-surgery), and 1 and 2-h post-surgery and analysed for circulating eicosanoids using liquid chromatography mass spectrometry (LCMS) methods. Measured levels of Thromboxane B₂ (TXB₂) at both 1 and 2 h and Prostaglandin E₂ (PGE₂) at 2 h post-surgery were higher in the Control group compared to the TD group, suggesting pre-operative application of TD ketoprofen has a possible inhibitory effect on systemic inflammation and could be used to treat pain and inflammation in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105512"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Scalibor® deltamethrin collar chronic exposure in dogs","authors":"Larissa T.P. Rodrigues ,&nbsp;Andriele F. Qualhato ,&nbsp;Carolle V.M. Sprícigo ,&nbsp;Kelly C.A. Arantes ,&nbsp;Rayanne H.S. da Silva ,&nbsp;Alexandre C.M. Barros ,&nbsp;Monica C. Vicente ,&nbsp;Murilo R. Souza ,&nbsp;Luiza G.F. de Paula ,&nbsp;Danilo Roman-Campos ,&nbsp;Artur S. Miranda ,&nbsp;Danieli B. Martins ,&nbsp;Ana F.M. Botelho","doi":"10.1016/j.rvsc.2024.105502","DOIUrl":"10.1016/j.rvsc.2024.105502","url":null,"abstract":"<div><div>Deltamethrin is a pesticide used worldwide with several applications in the control of nuisance arthropods in homes, agriculture, against ectoparasites in dogs and livestock. Recent studies indicate that deltamethrin can induce cardiotoxicity. Therefore, this study aimed to evaluate the toxicity of deltamethrin in dogs through exposure to commercial collars over 8 months. Twenty healthy dogs were selected, 9 males and 11 females, weighing between 10 and 15 kg and aged between 1 and 8 years, with no history of intentional exposure to pyrethroids. Clinical, cardiological, and laboratory evaluations were performed monthly. The results demonstrate that exposure to deltamethrin 4 % via the dermal route, through a commercial collar, was safe for 8 months, with preserved cardiac electrocontractile function, assessed by electrocardiogram and echocardiogram. In addition, tracking of plasma biochemical markers did not indicate signs of cardiac, renal, or hepatic injury. Although deltamethrin 4 % collars demonstrated an acceptable safety profile regarding cardiovascular and laboratory evaluations, the occurrence of dermatological reactions in 25 % of the animals, which is a significant proportion, highlights the need for careful monitoring of dermatological reactions. Future investigations with different concentrations and longer periods are recommended to achieve a more comprehensive understanding of the long-term effects of deltamethrin, improving its safe application in veterinary medicine and promoting animal and human health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105502"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence of lagomorpha coccidiosis from 1951 to 2024: A systematic review and meta-analysis","authors":"Huang-Liang Chen,&nbsp;Qi-Tao Zhang,&nbsp;Rui Qi","doi":"10.1016/j.rvsc.2024.105519","DOIUrl":"10.1016/j.rvsc.2024.105519","url":null,"abstract":"<div><div>The global prevalence of coccidia infection in lagomorphs and potential risk factors were investigated through a meta-analysis of 149 studies published between 1951 and 2024. The pooled prevalence of Eimeriidae, Sarcocystidae and Cryptosporidiidae was found to be 66.0 %, 8.0 % and 6.1 %, respectively. Rabbits and hares exhibited the highest prevalence of Eimeriidae at 63.1 % and 71.8 %, while pikas had the highest prevalence of Cryptosporidiidae at 17.1 %. Countries located in the southern hemisphere exhibited the highest prevalence of Cryptosporidiidae (11.4 %). The African Region had the highest prevalence of Eimeriidae (73.3 %) and Sarcocystidae(14.6 %), while the Eastern Mediterranean Region had the highest prevalence of Cryptosporidiidae(13.6 %). Juvenile rabbits exhibited the highest prevalence of Eimeriidae (67.4 %), while senior Rabbit exhibited the highest prevalence of Sarcocystidae(17.1 %) and Cryptosporidiidae(13.1 %). The prevalence of coccidia in intensively farmed lagomorphs showed a slight upward trend over time, nearly reaching statistical significance (R2 = 7.88 %, coefficient = 0.019, <em>P</em> = 0.127). This suggests that the current intensive farming practices may be losing effectiveness in controlling coccidia infections, which is concerning. Among Eimeriidae in lagomorphs, <em>Eimeria coquelinae</em> showed the highest prevalence (68.4 %), while <em>E. magna</em> was most frequently reported. For Sarcocystidae, <em>Besnoitia</em> spp. had the highest prevalence (31.3 %), with <em>Toxoplasma gondii</em> being most frequently reported. <em>Cryptosporidium cuniculus</em> was both the most prevalent and frequently reported Cryptosporidiidae species (6.9 %), suggesting it may be a major Cryptosporidiidae pathogen in lagomorphs. These findings highlight the differences in prevalence of various Eimeriida species among different lagomorpha genera. This diversity underscores the complex relationships between specific parasites and their host species, contributing to a more nuanced understanding of coccidiosis epidemiology in lagomorphs worldwide.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105519"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey on canine hookworms: Knowledge, perceptions and practices among U.S. veterinarians","authors":"Sara J. Ochoa ,&nbsp;Gregg A. Hanzlicek ,&nbsp;Kamilyah R. Miller ,&nbsp;Justin Kastner ,&nbsp;Jeba R.J. Jesudoss Chelladurai","doi":"10.1016/j.rvsc.2024.105501","DOIUrl":"10.1016/j.rvsc.2024.105501","url":null,"abstract":"<div><div>Canine hookworms (<em>Ancylostoma caninum</em>) are highly prevalent in pet dogs in the United States of America (U.S.). They pose a significant health risk to infected dogs and are also a potential risk to humans as zoonotic agents. Veterinarians play a crucial role in managing this threat through the diagnosis and treatment of infections in pet dogs. However, the rising incidence of anthelmintic resistance in U.S. hookworm populations necessitates increased awareness among veterinarians. To determine U.S. veterinarian's knowledge, perceptions and practices about canine hookworms, a cross-sectional online survey was administered between February and December 2022. 208 veterinarians from 43 states participated in the study. Only 65 % of veterinarians reported awareness of hookworm prevalence. Hookworm infected dogs were encountered more frequently in the South than in Western states. Veterinarians perceived that hookworms were present in all breeds without breed predilection followed by mixed breeds, pit bulls and hound breeds. Most respondents reported that infections were rarely symptomatic, highlighting the importance of diagnostic testing. In-clinic diagnostic modalities were most preferred. Fenbendazole and pyrantel pamoate were the most commonly used anthelmintics. Most veterinarians followed up on infected dogs but retesting time after treatment varied. Awareness of multi-drug resistant hookworms was high but encountering persistent hookworm cases was lower. Veterinarians frequently educated clients about hookworms and prevention, but less about the zoonotic potential. This study provides insights for improving awareness, diagnostics, treatment protocols, and client education regarding canine hookworms in the U.S.. Overall, veterinarians play an active role in hookworm prevention and control.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105501"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of prostatic androgen receptor and alpha-smooth muscle actin in castrated and non-castrated dogs","authors":"Sameh M. Farouk ,&nbsp;Amal A.M. Ahmed ,&nbsp;Mohamed A. Hashem ,&nbsp;Safa H. Badran","doi":"10.1016/j.rvsc.2025.105563","DOIUrl":"10.1016/j.rvsc.2025.105563","url":null,"abstract":"<div><div>The prostate, the only accessory sex gland in dogs, is androgen-dependent and has a vital influence on male fertility. Either for therapeutic or preventative purposes, orchidectomy is performed in both human and animal, especially for androgen-dependent disorders. Thereby, the current study aimed to evaluate the immunohistochemical characteristics of the canine prostate gland after unilateral and bilateral orchiectomy. Primarily, twenty-one healthy male dogs of 18–20 kg and 1–2 years were divided into three main groups; control, unilateral castrated and bilateral castrated groups. The last two groups were subdivided, based on post castration time, into three subgroups; 10, 20, and 30 days. The prostatic tissues were harvested from all experimental groups for immunohistochemical studies. The results revealed progressive significant alterations in the prostate gland immunostaining affinity after bilateral castration as time passed; gradual reduction in the androgen receptors and α-SMA immunoexpression was observed. On the contrary, the unilateral procedure conserves the prostatic tissue nearly similar to the control. It was concluded that, however, immunohistochemical characteristics of prostatic tissue were maintained without detectable changes after unilateral castration, there was a marked ongoing decrease of androgen receptors and α-SMA immunoreactivity post bilateral castration procedure.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105563"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143146623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring endosymbionts and pathogens in Rhipicephalus sanguineus and Ctenocephalides felis felis with Oxford Nanopore Technology","authors":"Luisa Páez-Triana ,&nbsp;David Martinez ,&nbsp;Luz H. Patiño ,&nbsp;Marina Muñoz ,&nbsp;Claudia M. Sandoval-Ramírez ,&nbsp;Juan Carlos Pinilla León ,&nbsp;Juan David Ramirez","doi":"10.1016/j.rvsc.2025.105562","DOIUrl":"10.1016/j.rvsc.2025.105562","url":null,"abstract":"<div><div>Fleas and ticks play a crucial role in public health as vectors of multiple diseases affecting humans and animals. Several rickettsial pathogens and endosymbionts are transmitted by fleas and ticks. Therefore, understanding this group of microorganisms is essential for fully grasping the spectrum of pathogens transmitted by vectors and the interactions between endosymbiotic microorganisms and their hosts. This study evaluated the presence and diversity of <em>Rickettsiales</em> species in fleas and ticks collected from the Santander department in Colombia. For the methodology a 16S gene amplification approach through Oxford Nanopore sequencing technologies in <em>Rhipicephalus sanguineus</em> and <em>Ctenocephalides felis felis</em> was used. Our findings revealed the presence of multiple pathogenic and endosymbiotic microorganisms, particularly from the <em>Rickettsia</em> and <em>Wolbachia</em> groups. We observed a clear association between <em>Rickettsia</em> species and ticks, while <em>Wolbachia</em> was predominantly found in fleas. Additionally, other important microorganisms were identified, including <em>Anaplasma phagocytophilum</em>, <em>Rickettsia conorii</em>, and different strains of <em>Wolbachia</em> that serve as endosymbionts in various arthropods. These results underscore the importance of fleas and ticks in the transmission of both pathogenic and endosymbiotic microorganisms. The distinct patterns of association between specific pathogens and vectors provide insight into their transmission dynamics. Identifying pathogens such as <em>Anaplasma phagocytophilum</em> and <em>Rickettsia conorii</em> further highlights the need for continued research into vector-borne diseases in Colombia. Understanding the interactions between endosymbionts and pathogenic microorganisms in these vectors could lead to the development of more effective strategies for controlling diseases transmitted by fleas and ticks.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105562"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143337940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Enterobacteriaceae from dairy cattle milk: A systematic review and meta-analysis","authors":"Dian Meididewi Nuraini ,&nbsp;Morsid Andityas ,&nbsp;Peerapol Sukon ,&nbsp;Patchara Phuektes","doi":"10.1016/j.rvsc.2024.105497","DOIUrl":"10.1016/j.rvsc.2024.105497","url":null,"abstract":"<div><div>Carbapenem-resistant <em>Enterobacteriaceae</em> (CRE) have been detected in dairy cattle milk, raising concerns about public health risks. This study aimed to assess the global prevalence of CRE in dairy cattle milk through a systematic review and meta-analysis, following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Retrieved articles from four databases were initially screened based on predefined inclusion criteria. The meta-analysis included 49 studies (2011–2024), covering 28,134 milk samples and 3462 <em>Enterobacteriaceae</em> isolates globally. Data from the full text were extracted to a Microsoft Excel spreadsheet and analysed using the ‘meta’ R package in R v.4.3.0 software for pooled prevalence and subgroup meta-analysis with a random-effects for logit transformation. Heterogeneity was assessed using Cochran's Q statistic (χ²), <em>p</em>-value and <em>I</em>^<em>2</em> statistic. Publication bias and sensitivity were evaluated using Egger's test, funnel plot, trim and fill plot, and leave-one-out test. Globally, the prevalence of CRE in dairy cattle milk was 0.73 % (95 % CI, 0.37–1.41). Subgroup meta-analysis based on continent, sample type, <em>Enterobacteriaceae</em> species, diagnostic method, antibiotic type, and interpretation guideline revealed no significant differences among the criteria within the subgroup. Although the overall pooled prevalence of CRE in dairy cattle milk is relatively low, it raises public health concern regarding raw milk consumption. This emphasizes the need for regular monitoring with in a One Health framework for CRE in the dairy industry to anticipate potential transmission between humans, animals, and the environment.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105497"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}