Research in veterinary science最新文献

{"title":"Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples","authors":"Vanina Saraullo ,&nbsp;Micaela Hamer ,&nbsp;Micaela Esteban ,&nbsp;Cristina Sanchez ,&nbsp;Bibiana Brihuega ,&nbsp;Mara Martinez","doi":"10.1016/j.rvsc.2025.105602","DOIUrl":"10.1016/j.rvsc.2025.105602","url":null,"abstract":"<div><div>Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105602"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to PFOS, PFBS, PFOA and PFBA impairs cell cycle progression in bovine brain (Bos taurus) endothelial cells","authors":"Alice Gonella ,&nbsp;Marta Giacomello ,&nbsp;Livio Finos ,&nbsp;Antonella Peruffo","doi":"10.1016/j.rvsc.2025.105585","DOIUrl":"10.1016/j.rvsc.2025.105585","url":null,"abstract":"<div><div>Perfluoroalkylated substances (PFAS) are the large class of synthetic chemicals that persist in the environment and bioaccumulate in different tissues including the brain, inducing blood brain barrier (BBB) disruption. In this study, we assessed cytotoxicity of PFAS in a bovine brain endothelial cell line by exposing the cells to increasing concentrations (0.01, 0.1, 1, 10, and 100 μM) of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorobutane sulfonic acid (PFBS), perfluorobutanoic acid (PFBA) and perfluoro ([5-methoxy-1,3-dioxolan-4-yl]oxy) acetic acid (C6O4). Cell viability, cell cycle profiling and the apoptotic potential were then analyzed. Cells were categorized by grouping nuclei into G0 + G1, Synthesis (S) and Mitotic phases (M), nuclei showing characteristics of senescence and nuclear fragments. By combining high throughput screening with cell nuclei counting for group, we determined the relationship between the dose-response effect of PFAS and their proliferative potential. Our results showed that PFOS decreased the number of cells in S and M phase. PFBS reduced the number of cells in M phase, decreased the senescence phenotype and increased the number of fragment nuclei. PFOA enhanced the number of cells nuclei in S and M phase. The PFBA enhanced the number of nuclei in G0 + G1, S and M phase. C6O4 did not show significant variation under any of the experimental conditions tested. We did not find significant changes in terms of cell viability assay. This bovine endothelial cell line provides an alternative model for studying the mechanisms involved in the decrease of BBB integrity due to PFAS accumulation in a large mammal with large brain.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105585"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doppler ultrasound for the assessment of renal perfusion in kittens up to three months of age and young adult cats","authors":"Maria Cristina Reis Castiglioni,&nbsp;Sheila Canevese Rahal,&nbsp;Jeana Pereira da Silva,&nbsp;Ricardo Shoiti Ichikawa,&nbsp;Maria Jaqueline Mamprim","doi":"10.1016/j.rvsc.2025.105590","DOIUrl":"10.1016/j.rvsc.2025.105590","url":null,"abstract":"<div><div>This study aimed to assess the Doppler-based renal resistive index (RI) and pulsatility index (PI) during the first three months of age and compare them with the indices of young adult cats. Forty-eight healthy mixed-breed cats were evaluated in this study, 24 males and 24 females. The client-owned cats and those from breeding catteries were distributed into six groups, each consisting of eight animals, based on their age: G1 - up to 10 days old, G2 - from 11 to 20 days of age, G3 - from 21 to 30 days of age, G4 - between 1 and 2 months of age, G5 - between 2 and 3 months of age, and G6 – between 1 and 3 years of age. <em>After B-mode ultrasound</em> of the right and left <em>kidney</em>s<em>,</em> values of RI and PI were determined with Doppler ultrasound of renal, interlobar, or arcuate arteries. Each artery was evaluated using spectral Doppler to obtain at least three similar, sequential, and complete Doppler waveforms. Only waveforms obtained when the cats were calm were used to obtain the RI and PI. These Doppler-based indices were automatically calculated using the ultrasound device after manual waveform delimitation. No statistical difference was found between the arteries, kidneys, or sexes, regardless of age (<em>P</em> &gt; 0.05). Mean values for RI and PI that included the three arteries were established. Statistical differences among all groups were observed (<em>P</em> &lt; 0.05). RI values in G1, G2, G3, G4, G5, and G6 were 0.75 ± 0.006, 0.73 ± 0.06, 0.70 ± 0.07, 0.66 ± 0.06, 0.67 ± 0.06, and 0.62 ± 0.070, respectively. PI values in G1, G2, G3, G4, G5, and G6 were 1.25 ± 0.196, 1.16 ± 0.182, 1.08 ± 0.193, 1.02 ± 0.177, 1.00 ± 0.160, and 0.97 ± 0.163, respectively. Comparing RI and PI among groups demonstrates the presence of differences between them (<em>P</em> &lt; 0.05), with higher values in G1 (up to 10 days of age) and a gradual drop in values up to G6 (young adults). A positive linear correlation was observed between RI and PI, with a coefficient greater than 0.80, regardless of the age group. In conclusion, the normal renal ultrasonographic <em>indices were age dependent,</em> being the highest values verified during the first month of age. The RI and PI reference values provided can be used for renal evaluation of kittens up to 3 months of age.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105590"},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Further insights into the hepatic metabolism of benzimidazole anthelmintics in sheep: Impact of dexamethasone-mediated induction of the cytochrome P450 3A pathway","authors":"Guillermo Virkel,&nbsp;Laura Maté,&nbsp;Paula Ichinose,&nbsp;Carlos Lanusse,&nbsp;Adrián Lifschitz","doi":"10.1016/j.rvsc.2025.105589","DOIUrl":"10.1016/j.rvsc.2025.105589","url":null,"abstract":"<div><div>The anthelmintics albendazole (ABZ) and fenbendazole (FBZ) undergo enantiospecific hepatic S‑oxygenation into sulfoxide metabolites, albendazole sulfoxide (ABZSO) and oxfendazole (OFZ), respectively. Flavin-monooxygenases (FMO) and cytochromes P450 (CYP) are involved in this process. While most is known on the FMO-dependent S‑oxygenation of both anthelmintics, the participation of different CYPs remains to be elucidated. This research investigated the role of the CYP3A pathway in the S‑oxygenation of ABZ and FBZ in sheep liver, particularly following induction with the glucocorticoid dexamethasone (DEX). Liver microsomes from DEX-treated (3 mg/kg/day for seven days) and untreated (control) sheep were prepared to determine monooxygenase activities and the involvement of the FMO and CYP pathways in the enantiospecific production of (+) and (−) sulfoxide enantiomers. DEX treatment significantly induced CYP3A marker catalytic activities, triacetyl-oleandomycin (TAO) and erythromycin (ERTM) <em>N</em>-demethylations [3.5-fold (<em>p</em> = 0.01) and 4.8-fold (<em>p</em> = 0.02), respectively]. Additionally, the FMO-dependent methimazole (MTZ) S‑oxygenase activity decreased by 28 % (<em>p</em> = 0.02) in DEX-treated sheep. A significant reduction in FMO-dependent production rates of (+) ABZSO (61 %, <em>p</em> &lt; 0.05) and (+) OFZ (71 %, p &lt; 0.05) was observed in DEX-treated sheep compared to controls. No changes in the FMO-mediated production of (−) enantiomers were observed between both experimental groups. DEX treatment induced the CYP-specific production of (−) ABZSO (2.5-fold, <em>p</em> = 0.03) and both (+) OFZ (1.8-fold, <em>p</em> = 0.04) and (−) OFZ (3-fold, p = 0.04). This work shows some distinctive enantiospecific metabolic differences between the two anthelmintic drugs and further contributes to understanding the pathways involved in their hepatic metabolism.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105589"},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New biomarkers and scintigraphic evaluation of renal function in dogs with canine leishmaniosis","authors":"Mario A. González ,&nbsp;Rafael Barrera-Chacón ,&nbsp;Joaquín Jiménez ,&nbsp;Silvia Belinchón-Lorenzo ,&nbsp;María Gil-Molino ,&nbsp;Nicolás R. Robles ,&nbsp;Francisco J. Duque","doi":"10.1016/j.rvsc.2025.105588","DOIUrl":"10.1016/j.rvsc.2025.105588","url":null,"abstract":"<div><div>This study evaluated the utility of new renal biomarkers in canine leishmaniosis (CanL). A total of 6 healthy dogs (CG) and 22 dogs with CanL (LeishVet stage III or GIII (<em>n</em> = 11) and stage IV or GIV (n = 11)) were included. Plasma creatinine, urea, cystatin C (pCysC), and symmetric dimethylarginine were analyzed. In urine, neutrophil gelatinase-associated lipocalin, retinol-binding protein, Tamm-Horsfall protein, and total protein were measured using their ratios with creatinine (uNGAL/c, uRBP/c, uTHP/c, and UPC, respectively). The estimated glomerular filtration rate (eGFR) was assessed by 99mTc-DTPA scintigraphy. All glomerular biomarkers strongly correlated with eGFR and showed statistically significant differences between CanL stages but not with CG, except for pCysC, which was particularly noteworthy for its statistical significance, instilling confidence in its potential use. All tubular biomarkers presented significant differences between GIV and CG; however, only uNGAL/c and uTHP/c differentiated between GIII and CG. Moreover, none could distinguish between the GIII and GIV groups. These data suggest that the new glomerular biomarkers could help determine stages of renal disease in CanL. uNGAL/c and uTHP/C showed advantages in making an earlier diagnosis. Ultimately, pCysC presented tremendous potential, as it was statistically significant for both uses. This research provides valuable information to understand the behavior of these biomarkers in renal disease secondary to CanL.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105588"},"PeriodicalIF":2.2,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rehabilitating wild animal welfare: A focus on veterinary rescue and rehabilitation interventions","authors":"Michaël Beaulieu","doi":"10.1016/j.rvsc.2025.105582","DOIUrl":"10.1016/j.rvsc.2025.105582","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105582"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Leptospira spp. in goat's milk produced in a semiarid region","authors":"Murilo Duarte de Oliveira ,&nbsp;Nathanael Natércio da Costa Barnabé ,&nbsp;João Paulo de Lacerda Roberto ,&nbsp;Maria Luana Cristiny Rodrigues Silva ,&nbsp;Sérgio Santos de Azevedo ,&nbsp;Clécio Henrique Limeira ,&nbsp;Clebert José Alves","doi":"10.1016/j.rvsc.2025.105584","DOIUrl":"10.1016/j.rvsc.2025.105584","url":null,"abstract":"<div><div>Brazil has a large flock of goats, and in the Northeast, there are around 94.5 % of the total flock, highlighting the states of Bahia, Ceará, Pernambuco, Piauí and Paraíba. Dairy goat farming based on the development of alternative models instigates the generation of income, the creation and strengthening of rural micro-enterprises linked to credit programs, aiming to reach quality in the products for the regional consumer market. Infectious diseases, such as leptospirosis, highlights regarding the drop in productivity, and, for detection of this agent, PCR (Polymerase Chain Reaction) is used as a direct diagnosis method. In the present work, molecular tests were carried out in 189 goat milk samples, collected in the dry period of 2019, from flocks of four municipalities in the backlands of Paraíba state, Brazil, five samples of collective cooling tank milk and four pasteurized milk samples. The primers <em>LipL</em>32-45F and <em>LipL</em>32-286R were used to amplify the gene <em>LipL</em>32, specific to pathogenic leptospires. The PCR technique detected the agent's DNA in 146 (77.24 %) goat milk samples; from collective tank, all five samples (100 %) showed <em>Leptospira</em> spp. DNA, so like the four pasteurized milk samples. The presence of leptospires in the mammary gland in lactating females is associated with the bacteremia phase of the infection, leading to a potential zoonotic risk in the consumption of raw milk by humans. Therefore, the goat milk production chain must consolidate efforts to improve milk quality, through the implementation of more effective health control measures and hygienic milking for flocks, including the use of monitoring and control tools, such as Hazard Analysis and Critical Control Point (HACCP), at all stages of goat milk production.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"187 ","pages":"Article 105584"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opsin 3, encoding a non-visual photoreceptor, is a pseudogene in cattle","authors":"Erina Kachi,&nbsp;Kei Kazama,&nbsp;Masaru Murakami,&nbsp;Ken Onda","doi":"10.1016/j.rvsc.2025.105586","DOIUrl":"10.1016/j.rvsc.2025.105586","url":null,"abstract":"<div><div>The non-visual photoreceptor opsin 3 (OPN3) performs various functions by directly accepting light and activating G protein-coupled receptor signalling in non-visual tissues. OPN3, which is expressed in brown adipocytes, induces the expression of uncoupling protein 1 to generate heat upon exposure to blue light. There have been few reports on OPN3 expression in ruminants, and the mRNA sequence registered in a database as bovine <em>OPN3</em> is the only predicted sequence. Therefore, we examined <em>OPN3</em> expression in cattle. In the bovine retina, amplified fragments of the visual opsins, rhodopsin and opsin 1 and the non-visual opsins, opsin 4 and opsin 5, were detected using reverse transcription PCR, whereas no amplified PCR products of <em>OPN3</em> were detected. Eleven tissues were used to analyse <em>OPN3</em> expression in the entire body of bovines, including the testis and placenta, where <em>OPN3</em> is highly expressed in humans and mice. No amplified PCR products were detected in any of the tissues. In a comparison among animal species, <em>OPN3</em> was found to be expressed in mouse and horse testes, whereas no expression was observed in cattle and goat tissues. RNA sequencing revealed no <em>OPN3</em> transcripts in the bovine placenta. Direct genomic DNA sequencing revealed a stop codon in the middle of the bovine <em>OPN3</em> sequence. These results suggest that bovine OPN3 is pseudogenic as it does not encode a complete receptor protein. They also indicated that OPN3 is pseudogenic not only in cattle but also in other Cetartiodactyla, including goats.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105586"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring trends in reproductive system microbiome research in farm animals: A bibliometric approach","authors":"Necati Esener","doi":"10.1016/j.rvsc.2025.105583","DOIUrl":"10.1016/j.rvsc.2025.105583","url":null,"abstract":"<div><div>This study presents a comprehensive bibliometric analysis of literature on the microbiome of reproductive systems in farm animals, utilizing data from the Web of Science (WoS) and Scopus databases. The primary aim was to evaluate the scientific content and bibliometric characteristics of articles published on the reproductive tract microbiome in farm animals. The advanced tool Bibliometrix was employed to explore bibliometric networks, providing insights into the scope of microbiome research within this field. Findings indicate that research in reproductive microbiomes is still emerging, with significant contributions from developed countries, particularly the United States, China, Brazil, and Spain. The field remains heavily focused on bovine studies, while substantial knowledge gaps persist regarding other livestock, including ovines, caprines, and camelids.</div><div>One of the most notable advancements in this area has been the adoption of next-generation sequencing (NGS), which has shifted microbiome research away from labour-intensive, culture-based methods to culture-independent, high-throughput approaches. Although NGS has significantly facilitated the study of bacterial communities within reproductive systems, limited research has been conducted on fungal and viral microbiomes. This bacterial emphasis is partly due to the accessibility and cost of NGS technologies, which remain prohibitively high for many institutions in developing regions, thus limiting participation. Clustering analysis identified three main themes: genetic influences on pregnancy, microbial-reproductive health interactions, and male fertility with a focus on sperm quality. Additionally, emerging research topics were identified, including microbial species associated with reproductive diseases in livestock.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105583"},"PeriodicalIF":2.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary study of neutrophils and neutrophil extracellular traps (NETs) in canine mammary tumors","authors":"Gulsum Karaman ,&nbsp;Volkan Ipek","doi":"10.1016/j.rvsc.2025.105573","DOIUrl":"10.1016/j.rvsc.2025.105573","url":null,"abstract":"<div><div>Neutrophils play a complex role in cancer biology, can contributing to tumor progression and immune defense. Neutrophil extracellular traps (NETs) have emerged as key modulators within the tumor microenvironment. Herein, the association between molecular classification, histological grade, necrosis, tumor-infiltrating neutrophils, and NETs was assessed in 19 canine mammary malignant tumors. Immunohistochemistry using citrullinated histone-3 (cith3) and myeloperoxidase (MPO) antibodies were used to detect NETs. A fading and re-staining method was applied on the same sections. NETs were scored based on the presence of cith3 positive areas and compared with tumor grade. The neutrophil score numerically increased as the tumor grade increased. The NET score was slightly higher in grade I carcinomas compared to carcinomas with other grades. On contrary, the necrosis score was also higher in grade II and III tumors than grade I tumors. A low but non-significant negative correlation existed between tumor grade and NET score (<em>r</em> = −0.219). No statistically significant associations between the tumor markers (ER, PR, HER2) and molecular subtypes with tumor grade, NET score, neutrophil count, and necrosis. In this study, the presence of NETs in canine malignant mammary tumor of different histological subtypes and grades was reported. Preliminary evidence was gathered that NETs are negatively correlated with tumor grade, suggesting their potential role in prognostication.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"186 ","pages":"Article 105573"},"PeriodicalIF":2.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}