犬肝细胞癌中miRNA转录组谱异常的鉴定及与人类HCC的比较

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-09-20 DOI:10.1016/j.rvsc.2025.105903

Saki Hashimoto , M.D. Nazmul Hasan , Mohammad Arif , Nobuhiro Nozaki , Al Asmaul Husna , Yu Furusawa , Takeshi Sogawa , Kaori Takahashi , Tomohide Kuramoto , Aki Noguchi , Masashi Takahashi , Md Mahfuzur Rahman , Naoki Miura

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引用次数: 0

摘要

肝细胞癌（HCC）是犬类中最常见的肝脏肿瘤。MicroRNA （miRNA）失调及其临床意义已在狗和人类癌症中报道，包括HCC。然而，确定整个miRNA转录组谱对于诊断和治疗至关重要。在这里，我们确定了犬HCC的miRNA转录组谱。首先，通过小RNA下一代测序（NGS）分析了三个对照肝组织和三个犬HCC患者的miRNA表达。在分析测序读数后，通过qRT-PCR进一步证实了临床样本和三种HCC细胞系中miRNA （DEmiRNA）的差异表达。从实验验证的人类mirna靶标数据库中分析了demirna的功能途径。我们的研究揭示了20个上调和9个下调的mirna。其中10个mirna通过qRT-PCR进一步验证。qRT-PCR和NGS显示相似的miRNA表达模式。此外，我们研究了基于KEGG通路分析的信号通路，并对犬HCC中失调的mirna进行了功能分析。上调的mirna主要在p53和细胞周期通路中发挥作用，并在DNA损伤修复和分化中发挥作用。相比之下，下调的mirna参与癌症和细胞周期途径，主要在细胞分化中起作用。此外，这些mirna可以在基于mirna的诊断和治疗中进行研究。

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Identification of dysregulated miRNA transcriptomic profile in canine hepatocellular carcinoma and comparison to human HCC

Hepatocellular carcinoma (HCC) is the most common liver tumor in dogs. MicroRNA (miRNA) dysregulation and its clinical significance have been reported in dog and human cancers, including HCC. However, determining the whole miRNA transcriptomic profile is essential for diagnosis and therapeutics. Here, we determined the miRNA transcriptomic profile of canine HCC. First, miRNA expression from three control liver tissues and three canine HCC patients was analyzed via small RNA next-generation sequencing (NGS). After analyzing the sequencing reads, differential miRNA (DEmiRNA) expression was further confirmed in the clinical samples and three HCC cell lines via qRT-PCR. The functional pathways of the DEmiRNAs were analyzed from an experimentally validated human miRNA-target database. Our study revealed 20 upregulated and nine downregulated miRNAs. Among them, 10 miRNAs were further validated by qRT-PCR. qRT-PCR and NGS revealed similar miRNA expression patterns. Furthermore, we investigated the signaling pathways based on the KEGG pathway analysis and performed a functional analysis of the dysregulated miRNAs in canine HCC. The upregulated miRNAs mainly play a role in the p53 and cell cycle pathways and function in DNA damage repair and differentiation. In contrast, the downregulated miRNAs are involved in cancer and cell cycle pathways and mainly function in cell differentiation. Further, these miRNAs may be investigated in light of miRNA-based diagnosis and therapies.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.