Research in veterinary science最新文献

{"title":"Tetracycline-resistant and -susceptible Chlamydia suis remain viable during manure processing","authors":"Daphne Zubler ,&nbsp;Hanna Marti ,&nbsp;Jiří Kratochvíl ,&nbsp;Nicole Borel","doi":"10.1016/j.rvsc.2026.106127","DOIUrl":"10.1016/j.rvsc.2026.106127","url":null,"abstract":"<div><div><em>Chlamydia suis</em> is commonly found in pigs and the only known chlamydial species to have acquired a tetracycline resistance gene, the <em>tetA</em>(C) gene. It has been established that both <em>tetA</em>(C)-positive and -negative <em>C. suis</em> strains can be isolated from rectal swabs of pigs, implying fecal shedding and fecal-oral transmission. However, little is known about the occurrence, viability and <em>tetA</em>(C) positivity of <em>C. suis</em> during manure processing. In this study, fresh feces and manure from slurry channels (storage up to two weeks), and manure silos (storage for several months until field application) from 30 pig farms across Switzerland were investigated regarding these parameters. Moreover, given the complex nature of feces and manure as matrices for molecular applications, three DNA extraction methods (Maxwell, MagPurix and DNeasy PowerSoil Pro Kit) were compared. Among these tested protocols, the DNeasy PowerSoil Pro Kit yielded the highest DNA quality and most consistent qPCR results. <em>C. suis</em> was detected and successfully isolated in cell culture from all investigated pig farms and sample types, demonstrating its ability to survive throughout the manure production process, with an isolation success rate of 100% (fecal samples), 96% (slurry channel) and 79% (silo). The viability of <em>C. suis</em> was further confirmed using viability PCR, which correlated well with cell culture isolation. Furthermore, the majority of isolated <em>C. suis</em> (66%) carried the <em>tetA</em>(C) gene, confirming the high prevalence of tetracycline resistance. The risk of spreading viable, tetracycline-resistant <em>C. suis</em> on the field is not eliminated by current manure management practices.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106127"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147322142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A standardized osteometric method for identifying Iberian raptors from skeletal remains","authors":"Andrea Miguel-Batuecas ,&nbsp;Juan A. De Pablo-Moreno ,&nbsp;Manuel Fuertes-Recuero ,&nbsp;Ariana Fuentes-Díaz ,&nbsp;Fernando González ,&nbsp;Laura Suárez ,&nbsp;Victoriano García-Matarranz ,&nbsp;Luis Revuelta","doi":"10.1016/j.rvsc.2026.106121","DOIUrl":"10.1016/j.rvsc.2026.106121","url":null,"abstract":"<div><div>Raptors suffer high mortality rates due to anthropogenic threats, such as electrocution from power lines or lead poisoning, so the specific identification of skeletal remains is essential for establishing monitoring and protection programmes.</div><div>This study developed and validated a standardized osteometric method for measuring long bones and craniopelvic bones from skeletal remains to identify raptor species. A database of 26 species of Iberian raptors (<em>n</em> = 853) was developed based on 11 measurements of 9 bones, including the ulna, sternum, femur, humerus, tarsometatarsus, tibiotarsus, and radius, as well as the synsacrum length and postacetabular ilium width and length and width of the skull. Missing values were estimated using multiple linear regression, and canonical discriminant analysis was applied to correct and modify the databases. Cross-validation was added to this analysis. Additionally, the obtained model was verified using external radiographs.</div><div>The highest percentage of success was obtained with the tarsometatarsus-ulna combination (94.53%), followed by tarsometatarsus-radius (94.05%) and tarsometatarsus-humerus (93.02%), when two bone lengths were combined. When a third measurement was incorporated, the tarsometatarsus-ulna-femur combination achieved a 99.45% correct classification rate. In blind verification testing, the radiographs of 15 individuals, the tarsometatarsus-ulna model correctly classified all samples, achieving a 100% success rate. These results show that the study method provides a simple and replicable protocol for identifying raptor species from incomplete skeletal remains. This optimizes the monitoring of mortality and supporting conservation measures.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"203 ","pages":"Article 106121"},"PeriodicalIF":1.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cluster analysis of veterinarians' antimicrobial prescription preferences for companion animals in Portugal","authors":"G.L.C. Alcântara ,&nbsp;K. Pinello ,&nbsp;M. Severo ,&nbsp;J. Niza-Ribeiro","doi":"10.1016/j.rvsc.2026.106070","DOIUrl":"10.1016/j.rvsc.2026.106070","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial stewardship is essential to tackle antimicrobial resistance, particularly in veterinary practice where antimicrobials of high importance for human health require careful oversight. This study assessed prescribing patterns and concordance with FECAVA recommendations for prudent antimicrobial use across common clinical scenarios, providing a Europe-wide framework for responsible antimicrobial prescribing in companion animals.</div></div><div><h3>Methods</h3><div>A cross-sectional survey was conducted among veterinarians in Portugal (October 2019–January 2020), covering 11 clinical scenarios and 18 therapeutic options, including ‘no antimicrobial therapy’. Descriptive, inferential, and cluster analyses were performed.</div></div><div><h3>Results</h3><div>Among 416 respondents, amoxicillin–clavulanic acid (27.4%) and fluoroquinolones (13.8%) were most frequently chosen. Six prescribing clusters were identified: Cluster D (33.2%) was the largest, followed by Clusters C (23.3%) and F (22.1%). Younger veterinarians (&lt;42 years) were more likely to belong to Cluster A (<em>p</em> &lt; 0.001). Clusters A and F frequently selected ‘no antimicrobials,’ whereas Cluster B predominantly relied on amoxicillin–clavulanic acid and aminoglycosides. Concordance with FECAVA recommendations was highest in Cluster A and lowest in Cluster B. Importantly, consensus across clusters was observed for post-operative prophylaxis, wound treatment, and osteomyelitis, where prescription patterns aligned with FECAVA guidance.</div></div><div><h3>Limitations</h3><div>Limitations include potential recall and social desirability bias inherent to self-reported data, and the hypothetical scenarios did not account for case severity or previous antimicrobial exposure.</div></div><div><h3>Conclusion</h3><div>Six distinct prescribing profiles were identified, with broad-spectrum antimicrobials frequently selected. The variability in antimicrobial prescription recommendation between the profiles underscores the need for targeted antimicrobial stewardship interventions to strengthen adherence to guidelines in companion animal practice.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106070"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumpy skin disease threat in Europe: Current situation, transmission dynamics and future prospects","authors":"Kadir Yeşilbağ ,&nbsp;Eda Baldan Toker ,&nbsp;Mevlüt Yaşar ,&nbsp;Jordi Casal ,&nbsp;Annamaria Pratelli","doi":"10.1016/j.rvsc.2026.106061","DOIUrl":"10.1016/j.rvsc.2026.106061","url":null,"abstract":"<div><div>Lumpy skin disease (LSD), caused by Lumpy Skin Disease Virus (LSDV, genus <em>Capripoxvirus</em>), is an emerging transboundary disease of cattle and water buffalo. Although rarely fatal, it results in severe economic losses due to decreased productivity, hide damage, infertility, and trade limitations. This review summarizes the etiology, susceptible hosts, modes of transmission, and advances in control, vaccination, and treatment strategies, with a particular focus on Europe, where spread into previously unaffected areas has recently been reported. Since its first identification in Zambia in 1929, LSD remained endemic in Africa for six decades before spreading into the Middle East, Asia, and Europe. Major outbreaks have subsequently occurred in Türkiye, the Balkans, Russia, Asian territories, and, most recently, in Italy, France, and Spain in 2025. Transmission is primarily vector-borne through blood-feeding arthropods, while animal movements and inadequate farm-level biosecurity further exacerbate the spread. Control measures such as vaccination with homologous live-attenuated vaccines, quarantine, and movement restrictions have proven effective in limiting outbreaks. LSD poses a significant threat to livestock health and trade globally. Coordinated surveillance, improved biosecurity, and vaccination remain the cornerstones of control. Continued research into antiviral and alternative therapeutic strategies to complement existing prevention measures is warranted. Killed vaccines may currently help to protect animals in pre-epidemic areas and slow the rate of epidemics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106061"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure of the nucleoli in the spermatocytes of three canid species","authors":"Katarzyna Andraszek ,&nbsp;Marta Kuchta-Gładysz ,&nbsp;Piotr Niedbała","doi":"10.1016/j.rvsc.2026.106063","DOIUrl":"10.1016/j.rvsc.2026.106063","url":null,"abstract":"<div><div>The nucleolus is a structure of the cell nucleus associated mainly with ribosome biogenesis and indirectly responsible for protein biosynthesis. The nucleolus also plays a role in the regulation of cell growth and cell cycle progression, the detection and repair of DNA damage, and the export or degradation of mRNA. It is also involved in the most critical processes for the organism, such as ageing at the cellular level and broadly defined carcinogenesis. Analysis of the structure of nucleoli, which are the products of nucleolar organizer regions, can be an alternative source of information on the activity of rRNA-encoding genes. The material for the study was testes collected post mortem from sexually mature males of three canid species: silver fox (<em>Vulpes vulpes</em>), blue fox (<em>Alopex lagopus</em>) and Chinese raccoon dog (<em>Nyctereutes procyonoides procyonoides</em>). The morphology and morphometry of nucleoli identified in early primary spermatocytes were analysed. The values for all morphometric parameters of the spermatocyte nucleus were lowest for the silver fox and highest for the raccoon dog. In the case of nucleolus parameters, the lowest values were found in the raccoon dog and the lowest in the blue fox. The size of the nucleoli and spermatocyte nucleus proved to be a species-specific trait associated with the karyotype and number of nucleolar organizer regions in the species. The nucleolus is believed to play an important role in controlling meiosis. It is involved in preventing chromosome segregation during crossing over, and proteins associated with the nucleoli identify damaged synaptonemal complexes. Epigenetic regulations at the level of the nucleolus and rRNA genes remain a crucial subject of genetic research.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106063"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of fresh frozen plasma immunotherapy as an adjuvant treatment of neonatal sepsis in puppies","authors":"Keylla Helena Nobre Pacífico Pereira ,&nbsp;Kárita da Mata Fuchs ,&nbsp;Gleice Mendes Xavier ,&nbsp;Júlia Cosenza Mendonça ,&nbsp;Diogo Ribeiro Câmara ,&nbsp;Luiz Eduardo Cruz dos Santos Correia ,&nbsp;Paulo Fernandes Marcusso ,&nbsp;Fabiana Ferreira de Souza ,&nbsp;Márcio Garcia Ribeiro ,&nbsp;Maria Lucia Gomes Lourenço","doi":"10.1016/j.rvsc.2026.106085","DOIUrl":"10.1016/j.rvsc.2026.106085","url":null,"abstract":"<div><div>The aim of this study was to evaluate the effects of fresh frozen plasma (passive immunotherapy) on clinical and laboratorial findings in puppies treated for neonatal sepsis. A total of thirty-one puppies were included in this study, 16 were diagnosed with sepsis and treated either with plasma + antibiotic (plasma group, <em>n</em> = 7; PG) or antibiotic only (control group, <em>n</em> = 9; CG); whereas 15 were healthy neonates (HG). The groups were evaluated at the first appointment (M0), after 24 (M24), 48 (M48), 72 h (M72) and at the end of treatment (ME). At M0, neonates with sepsis presented a reduction (<em>p</em> &lt; 0.05) in heart rate (HR), reflexes, blood glucose, body temperature, and leukocytes (leukopenia), with an increase (p &lt; 0.05) in lactate and troponin I, compared to HG puppies. During treatment, significant improvement of laboratory parameters was observed in PG compared to CG puppies, with higher IgM level at M24, and higher blood glucose and leukocyte count at M24 and M48 (<em>p</em> &lt; 0.05). At ME, the lactate was lower in PG than CG puppies (p &lt; 0.05). There were no differences in the serum IgG or IgA concentrations among groups. Evaluating clinical and laboratory improvement between time points within the same group, differences (<em>p</em> &lt; 0.05) were observed in the PG puppies, which increased blood glucose, temperature, leukocytes count, and IgM at M24 compared to M0. In the CG, a decrease in IgM concentration was detected at M24 compared to M0 (<em>p</em> &lt; 0.05). Approximately 57% (4/7) of the neonates in the PG presented leukograms without alterations at M24. In the CG, leukograms without alterations were observed only at M72 in 55.5% (5/9) of the animals. The mortality rate was 22% (2/9) in the CG. There was no mortality in the PG. This was the first study using fresh frozen plasma as an adjuvant in the treatment of neonatal sepsis in dogs and evaluation of biomarkers in diagnosis. It was concluded that sepsis led to changes in the physiological parameters of neonatal dogs, demonstrating that the evaluation of leukocytes, troponin I and lactate can be used in the diagnosis and monitoring of sepsis. It was concluded that, besides being an important source of IgM, fresh frozen plasma stimulated healing of puppies with neonatal sepsis, by prompt (within 24 h) improvement in both clinical and laboratory parameters. Plasma therapy has been shown to be beneficial in the treatment of sepsis and is a promising therapeutic strategy for reducing neonatal mortality.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106085"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence, molecular characterization, biotyping, and associated risk factors of bovine viral diarrhea in dairy cattle in Bangladesh","authors":"Eaftekhar Ahmed Rana ,&nbsp;Md Saiful Islam ,&nbsp;Belayet Hossain ,&nbsp;Abdul Ahad ,&nbsp;David J. Hampson ,&nbsp;Sam Abraham ,&nbsp;Subir Sarker ,&nbsp;Jully Gogoi-Tiwari ,&nbsp;Jasim M. Uddin","doi":"10.1016/j.rvsc.2026.106065","DOIUrl":"10.1016/j.rvsc.2026.106065","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) is globally endemic, with the ability to establish persistent infection (PI) being central to its complex epidemiology. Currently the genetic variability of BVDV in Bangladesh remains poorly understood. This study involved a survey in commercial dairy herds in the south-eastern part of Bangladesh in 2024/2025. A total of 373 blood samples were collected from cattle in 24 dairy herds. Serum and buffy coat samples were analyzed using antibody-ELISA and RT-qPCR targeting the 5′-UTR region, followed by sequencing. The MDBK cell line was used for virus isolation and biotyping. Herd and animal-level seroprevalences were 83.3% and 15.3%, respectively, while the corresponding viremic rates were 79.2% and 11.0%. Analysis of 41 sequences identified nine distinct BVDV-1 subgenotypes (1a, 1b, 1c, 1d, 1e, 1 k, 1p, 1o, and 1v), with BVDV-1b (41.5%) and BVDV-2a (14.6%) predominating. Additionally, five HoBiPeV-a pestiviruses were detected. Among antigen-positive cattle, 38 (92.68%) were identified as transiently infected and 3 (7.3%) were confirmed as PI. Six (14.6%) and 27 (65.9%) were identified as cytopathic and non-cytopathic biotypes, respectively. Risk factors for BVDV seropositivity included: female sex (OR: 3.0), clinical disease in the past three months (OR: 2.4), crowding (OR: 2.9), and lack of dedicated clothing for farm workers (OR: 5.7). Active infection was associated with calves (OR: 6.2), heifers (OR: 2.3), stunted growth (OR: 3.0), technician-performed artificial insemination (OR: 10.4), and frequent neighboring farm visits (OR: 3.1). This study has provided data crucial for formulating prevention and control strategies against BVDV to safeguard the Bangladeshi dairy industry.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106065"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146012063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological factors of Eucoleus aerophilus infection in red foxes (Vulpes vulpes) from semi-arid Mediterranean environments","authors":"I. Arcenillas-Hernández ,&nbsp;M. Català-Barrasetas ,&nbsp;P. Pérez-Cutillas ,&nbsp;M.R. Ruiz de Ybáñez ,&nbsp;C. Martínez-Carrasco","doi":"10.1016/j.rvsc.2026.106064","DOIUrl":"10.1016/j.rvsc.2026.106064","url":null,"abstract":"<div><div><em>Eucoleus aerophilus</em> is a ubiquitous zoonotic nematode found in the tracheobronchial mucosa of the definitive hosts, described in wild and domestic canids, including the red fox (<em>Vulpes vulpes</em>). To assess <em>E. aerophilus</em> occurrence and intensity in red foxes, the respiratory system of 126 foxes from Region of Murcia (SE, Spain) were examined. Statistical analyses were performed to investigate the influence of biotic and abiotic factors on the parasite's occurrence. The occurrence of <em>E. aerophilus</em> was 28.6% (95% CI: 20.6–36.4), with a mean intensity of 3.1 nematodes per parasitised fox. The infection rate and parasite intensity were higher in forested areas, suggesting that earthworm abundance and a longer survival of infective <em>E. aerophilus</em> eggs increase the risk of transmission and maintenance in the environment. Moreover, an inverse relationship between <em>E. aerophilus</em> occurrence and temperature (<em>p</em> &lt; 0.05) was observed. Results demonstrated that foxes participate in maintaining the cycle of <em>E. aerophilus</em> in semi-arid Mediterranean environments. This should be taken into account at the wild-domestic-human interface. The development of a predictive model of <em>E. aerophilus</em> infection risk in red foxes will allow the design of future strategies for the prevention of capillariosis, especially in areas where contact between foxes, domestic carnivores, and humans exists.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106064"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146012034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroretinographic measures and their correlation with retinal vascular calibers in bitches with pyometra-induced systemic inflammatory response syndrome","authors":"Fábio Dumit Pizzinatto ,&nbsp;Alexandre Pinto Ribeiro ,&nbsp;Bianca Eidt Rodrigues ,&nbsp;Emilio Fernandes Rodrigues Junior","doi":"10.1016/j.rvsc.2026.106067","DOIUrl":"10.1016/j.rvsc.2026.106067","url":null,"abstract":"<div><div>Pyometra is characterized by a uterine inflammatory process that leads to systemic inflammatory response syndrome (SIRS). Bitches with pyometra-induced SIRS (P-SIRS) develop marked retinal microvascular alterations that may affect ERG findings. Therefore, this study aimed to assess potential changes in implicit times, wave amplitudes in pyometra-induced SIRS. Furthermore, we evaluated possible correlations between retinal vascular calibers and ERG parameters in the same patients. A prospective observational study was conducted on 14 bitches diagnosed with P-SIRS and 10 clinically healthy bitches (CG). In the P-SIRS group, the caliber of the retinal arterioles was significantly smaller (<em>p</em> = 0.0004), whereas venules were significantly larger (<em>p</em> = 0.008). The amplitude of the a-wave in the mixed rod–cone response was significantly smaller in the P-SIRS group compared to the CG (<em>p</em> = 0.03). The a-wave implicit times in the mixed rod–cone response were negatively correlated with both retinal arteriolar (<em>p</em> = 0.01) and venular (<em>p</em> = 0.03) calibers (Table 5; Fig. 3). Positive correlations were also observed between arteriolar (<em>p</em> = 0.04) and venular (<em>p</em> = 0.01) calibers and photoreceptor negative response (PhNR). This study demonstrated that retinal arteriolar constriction and venular dilation in pyometra-induced SIRS reduces the a-wave amplitude in the mixed rod–cone response. The observed correlations between retinal vascular calibers and the implicit times of the a- and b-waves, as well as between PhNR and vessel calibers, suggest that SIRS-induced changes in retinal vasculature can alter retinal blood flow, which is reflected in ERG parameters.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106067"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrauterine oxygen/ozone mixture for the treatment of subclinical endometritis in repeat breeder cows","authors":"Alice Carbonari ,&nbsp;Matteo Burgio ,&nbsp;Lorenza Frattina ,&nbsp;Vincenzo Cicirelli ,&nbsp;Fabrizio Iarussi ,&nbsp;Maria Tempesta ,&nbsp;Maria Stella Lucente ,&nbsp;Annalisa Rizzo ,&nbsp;Grazia Greco","doi":"10.1016/j.rvsc.2026.106075","DOIUrl":"10.1016/j.rvsc.2026.106075","url":null,"abstract":"<div><div>Repeat Breeder Syndrome is a multifactorial condition in which subclinical endometritis (SCE) may represent an underlying cause. The aim of this study was to evaluate the efficacy of an intrauterine oxygen/ozone gas mixture administered at heat in Repeat Breeder (RB) cows with SCE. Of 156 RB cows, 57 affected with SCE based on cytological evaluation using the cytobrush technique were enrolled in the controlled clinical trial. These cows were randomly allocated, using randomization software, into two groups including treated (GM; 30) and untreated (CON; 27) animals. The intrauterine bacteria occurrence was monitored before (T0) and 12 h (T12) after treatment, along with an evaluation of reproductive parameters after the 1st and 2nd artificial insemination (AI). At T0, both groups showed similar bacterial positivity rates (12/30, 40% GM vs. 14/27, 51.85% CON; <em>p</em> = 0.43). At T12, all GM-positive cows had cleared cultivable bacteria, whereas all CON-positive cows remained bacteriological positive (<em>p</em> = 0.00001). This result indicated a 100% in-vivo clearance of cultivable bacteria. Furthermore, increasing pregnancy percentages were recorded after the 1st and 2nd AI in GM group (9/30, 30% and 9/21, 43%, respectively; <em>p</em> &lt; 0.05) compared to the CON group (2/27, 7% and 1/25, 4%, respectively; <em>p</em> &lt; 0.001).</div><div>Overall, these preliminary findings suggest that intrauterine ozone therapy may represent a promising complementary tool for the management of SCE in RB cows, although further studies are required to confirm its efficacy and long-term benefits.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"202 ","pages":"Article 106075"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}