Research in veterinary science最新文献

{"title":"Histological differences between brachycephalic and normocephalic dog palatine tonsils","authors":"Isabella A. Smith ,&nbsp;Philip A. Moses ,&nbsp;William B. Moses ,&nbsp;Jamie Fu ,&nbsp;Xena Yap ,&nbsp;Daniel McGilp ,&nbsp;Michelle M. Story ,&nbsp;Rachel Allavena","doi":"10.1016/j.rvsc.2025.105733","DOIUrl":"10.1016/j.rvsc.2025.105733","url":null,"abstract":"<div><h3>Objective</h3><div>To compare the histological morphology of palatine tonsils from brachycephalic and normocephalic dogs.</div></div><div><h3>Material and methods</h3><div>Surgically resected palatine tonsils from 116 dogs undergoing corrective surgery for brachycephalic obstructive airway syndrome (BOAS) were received from a veterinary specialist clinic. Control tonsils were opportunistically collected at autopsy from 11 normocephalic dogs that died of diseases unrelated to the upper respiratory tract. The tonsils were sectioned longitudinally and prepared using standard histological techniques. Digital microscopy was used to obtain standardised measurements of tonsillar epithelium and lymphoid follicles, and the means of these measurements were then compared between the two groups.</div></div><div><h3>Results</h3><div>Epithelial width and lymphoid follicle area were significantly greater for brachycephalic tonsils. Mean epithelium width was 48 % thicker (<em>p</em> &lt; 0.0001) and mean lymphoid follicle area was 52 % larger (<em>p</em> = 0.0019) for brachycephalic tonsils compared to normocephalic controls. Inflammation, indicated by neutrophil migration within the epithelium, was found in 54 % of brachycephalic tonsils but was absent in controls (<em>p</em> = 0.0012).</div></div><div><h3>Conclusions</h3><div>Significant histopathological differences were found in brachycephalic palatine tonsils compared to normocephalic controls. Changes identified in brachycephalic tonsils included lymphoid and epithelial hyperplasia, and neutrophilic inflammation, which we postulate are secondary to airway irritation caused by BOAS. As these pathological changes in the tonsils are potentially reversible, we hypothesise that they may resolve if the primary BOAS abnormalities are surgically corrected, rendering tonsillectomy unnecessary. Therefore, further research is needed to understand how hyperplastic tonsillar tissue responds to correction of primary BOAS abnormalities, and if tonsillectomy is a necessary component of BOAS corrective surgery.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105733"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breed predispositions and malignancy analysis for canine tumors: A multicenter histopathological retrospective study from Central Italy","authors":"Niccolò Fonti ,&nbsp;Azzurra Carnio ,&nbsp;Cristiano Cocumelli ,&nbsp;Elena Sophie Dhein ,&nbsp;Claudia Eleni ,&nbsp;Valentina Galietta ,&nbsp;Franco Guscetti ,&nbsp;Alessio Lachi ,&nbsp;Francesca Parisi ,&nbsp;Alessandro Poli ,&nbsp;Paola Scaramozzino ,&nbsp;Francesca Millanta","doi":"10.1016/j.rvsc.2025.105752","DOIUrl":"10.1016/j.rvsc.2025.105752","url":null,"abstract":"<div><div>Animal Cancer Registries (ACRs) enhance our understanding of oncology by providing insights into epidemiologic and clinical trends and fostering comparative research. This study analyzed a large dataset of canine tumors, evaluating their distribution and malignancy profiles by breed, sex, neuter status, age, and district of origin. Histologically diagnosed tumors from two pathology-based ACRs in central Italy (2008–2023) were coded using Vet-ICD-O-canine-1. Logistic regression assessed the influence of variables on tumor behavior, modeled as a binary outcome (“malignant” vs “benign”). Of 26,218 tumors, 41.6 % were benign and 58.4 % malignant. Malignancy risk increased by 8 % per year of age. Females had higher risk of malignancy (OR = 2.16; 95 %CI 2.04–2.29). Neuter status didn't affect malignancy risk (OR = 1.02; 95 %CI 0.96–1.09) but influenced risk for specific tumor groups. Highest malignancy risk was in Dogo Argentinos (OR = 2.18; 95 %CI 1.49–3.25), Rottweilers (OR = 2.00; 95 %CI 1.56–2.57), Pugs (OR = 1.99; 95 %CI 1.38–2.92), and Doberman Pinschers (OR = 1.79; 95 %CI 1.35–2.40); lowest in West Highland White Terriers (OR = 0.54; 95 %CI 0.38–0.76), Siberian Huskies (OR = 0.66; 95 %CI 0.49–0.90), Cocker Spaniels (OR = 0.69; 95 %CI 0.58–0.83), and Poodles (OR = 0.72; 95 %CI 0.60–0.87). Potential new predispositions emerged in Cane Corsos (lymphomas, basal cell tumors, histiocytic tumors) and Maremma Sheepdogs (odontogenic, adnexal, and skin appendage neoplasms). This study confirms known patterns, reveals novel risk factors, and highlights the importance of multicenter collaboration in identifying high-risk oncology patients.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105752"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144261375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose- and time-dependent effects of intravenous irisin administration on GnRH, LH, FSH, and estrogen in Kurdish ewes during the breeding season","authors":"Borhan Shokrollahi ,&nbsp;Farshad Sharifi","doi":"10.1016/j.rvsc.2025.105737","DOIUrl":"10.1016/j.rvsc.2025.105737","url":null,"abstract":"<div><div>Efficient reproduction in seasonally breeding ewes hinges on a precise integration of metabolic cues and the hypothalamic–pituitary–gonadal (HPG) axis. This study investigated the dose- and time-dependent effects of intravenous administration of irisin on reproductive (GnRH, LH, FSH, and estrogen) and metabolic (glucose, insulin, leptin) hormones in Kurdish ewes during the breeding season. Twenty-four clinically healthy ewes were randomly assigned to four groups (<em>n</em> = 6 each): Control (saline), Low-dose (10 ng/kg), Medium-dose (20 ng/kg), and High-dose (40 ng/kg) irisin. Treatments were administered every three days for 45 days, and blood samples were collected up to Day 60. Estrous cycle phase (luteal or follicular), confirmed by progesterone measurements and behavioral observations, did not differ among groups. Medium- and high-dose irisin elicited significant increases in GnRH, LH, FSH, and estrogen concentrations, particularly on Days 30 and 45, whereas low-dose irisin produced minimal endocrine effects. Insulin and leptin also increased markedly in the medium- and high-dose groups, suggesting a parallel dose-related impact on metabolic pathways. By Day 60, reproductive hormone levels returned near baseline, indicating that continuous irisin exposure is required to sustain these changes. Correlation analyses revealed that insulin and leptin are intricately linked to gonadotropin and estrogen variations, underscoring irisin's role as a metabolic-reproductive mediator. These findings imply that exogenous irisin can modulate reproductive function in ewes by coordinating metabolic and endocrine signals, offering potential applications for managing fertility in seasonally breeding ruminants.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105737"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TYROBP modulates NFκB phosphorylation and SQSTM1 expression in macrophages during Brucella Abortus LPS infection","authors":"Wenping Wu ,&nbsp;Wenbo Chen ,&nbsp;Shicheng Wan ,&nbsp;Xuan Luo ,&nbsp;Donghui Yang ,&nbsp;Congliang Wang ,&nbsp;Haijing Zhu ,&nbsp;Haisheng Yu ,&nbsp;Na Li ,&nbsp;Jinlian Hua","doi":"10.1016/j.rvsc.2025.105750","DOIUrl":"10.1016/j.rvsc.2025.105750","url":null,"abstract":"<div><div>TYROBP (also known as DAP12), a transmembrane signaling adaptor protein, activates downstream signaling cascades through its immunoreceptor tyrosine-based activation motif (ITAM) by recruiting tyrosine kinase SYK. TYROBP plays an important role in various immune responses, including inflammation and phagocytosis. Although the role of TYROBP in defense against bacterial infections has been partially investigated, its mechanism in <em>Brucella</em> infection is unclear. Using <em>Tyrobp</em>-knockout RAW264.7 macrophages, we demonstrated that gene deletion impaired macrophage phagocytosis. Following <em>B. abortus</em> lipopolysaccharide (LPS) stimulation, <em>Tyrobp</em> deficiency exacerbated mitochondrial damage and enhanced NF-κB phosphorylation. Quantitative proteomics identified SQSTM1 as a TYROBP-interacting partner, and its expression in knockout cells was reduced under LPS treatment conditions in Western blot results (<em>P</em> = 0.0887). RNAi-mediated SQSTM1 depletion phenocopied TYROBP ablation, recapitulating mitochondrial dysfunction and NF-κB hyperactivation. These results suggest that TYROBP inhibits NFκB phosphorylation through upregulation of SQSTM1, affecting macrophage activation and intracellular bacterial clearance. The study also suggests that the TYROBP-SQSTM1-NFκB signaling pathway may play a key role in <em>Brucella</em> infection, providing a new perspective for understanding the mechanism of <em>Brucella</em> infection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105750"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of SSEA-3-positive mesenchymal stem cells from equine bone marrow and evaluation of their pluripotency","authors":"Sangchan Lee ,&nbsp;Mya Theingi Kyaw ,&nbsp;Koji Harada ,&nbsp;Ken Takeshi Kusakabe ,&nbsp;Masaya Igase ,&nbsp;Naoki Sasaki","doi":"10.1016/j.rvsc.2025.105736","DOIUrl":"10.1016/j.rvsc.2025.105736","url":null,"abstract":"<div><div>Generally, most mesenchymal stem cells (MSCs) have lower pluripotency and limited differentiation potential than embryonic stem cells (ESCs). However, a small subpopulation of MSCs, called multilineage differentiating stress-enduring (MUSE) cells, exhibit pluripotency. MUSE cells express stage-specific embryonic antigen 3 (SSEA-3), a sphingoglycolipid. Here, we isolated and investigated the pluripotency of SSEA-3-positive MSCs (MUSE cells).</div><div>Six thoroughbred horses were used as test subjects. MSCs were harvested from the bone marrow of the thoracic vertebrae under ultrasound guidance. Harvested MSCs were sorted into SSEA-3-positive and -negative cells using a cell sorter, and their pluripotency was evaluated by immunostaining for the markers NANOG, OCT4, and SOX2.</div><div>The results showed that 2.1 % of equine MSCs were SSEA-3-positive, and these cells were successfully isolated using a cell sorter. This study confirms that SSEA-3-positive cells are present in equine bone marrow-derived MSCs, similar to that in other species.</div><div>Immunostaining revealed SOX2 (88.3 ± 11.6 %) and NANOG expressions (9.0 ± 3.9 %) in the SSEA-3-negative cell group, whereas OCT4 expression was not detected (0.0 ± 0.0 %). Contrastingly, the SSEA-3-positive cell group showed significant NANOG (19.8 ± 3.8 %), OCT4 (9.7 ± 2.6 %), and SOX2 expressions (97.3 ± 4.1 %), with significantly higher values for NANOG and OCT4 compared to the SSEA-3-negative group (<em>p</em> &lt; 0.05).</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105736"},"PeriodicalIF":2.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facial action units as biomarkers of postoperative pain in ovariohysterectomized bitches treated with cannabidiol and meloxicam","authors":"Alejandro Casas-Alvarado ,&nbsp;Julio Martínez-Burnes ,&nbsp;Patricia Mora-Medina ,&nbsp;Ismael Hernández-Avalos ,&nbsp;Agatha Miranda-Cortes ,&nbsp;Adriana Domínguez-Oliva ,&nbsp;Daniel Mota-Rojas","doi":"10.1016/j.rvsc.2025.105734","DOIUrl":"10.1016/j.rvsc.2025.105734","url":null,"abstract":"<div><div>Facial expression in animals is a suggested method to assess the subtle facial changes associated with pain. This study aimed to evaluate postoperative pain and establish its correlation with facial action units (AUs) in female dogs undergoing elective ovariohysterectomy (OVH) treated with cannabidiol alone or in combination with meloxicam. Sixty healthy female dogs of different breeds, were randomly assigned into four groups according to the treatment. The control group (G₀: <em>n</em> = 15) received a saline solution as placebo; the meloxicam group (G_Mlx: n = 15) received meloxicam at a dose of 0.2 mg kg⁻¹ IV, followed by 0.1 mg kg⁻¹ every 24 h, for 48 h during the postoperative period; the cannabidiol group (G_CBD: <em>n</em> = 15) received 2 mg kg⁻¹ PO every 12 h; and the combination group (G_Mlx/CBD: n = 15) received both treatments. The treatments were administered 30 min before starting the surgery. High-resolution videos were recorded for one minute at baseline and multiple time points up to 48 h post-surgery to quantify facial action units: AU101, AU143 + 145, AU12, ear position, facial muscle tension, orbital tightening, and lip tension. Data was analyzed with a mixed linear model. The frequency of AU143 + 145, AU12, ear position, tension of the facial muscles, orbital tightening, and tension of the lips significantly increased at 30 min, 1 h, 2 h, and 3 h post-surgery (<em>p</em> = 0.0001) in the control group. Thus, facial expressions associated with acute pain in dogs were characterized by AUs such as blink (AU143 + 145), lip corner puller (AU12), ear position, head position, tension of the facial muscles, orbital tightening, and tension of the lips, which maintained a positive moderate correlation with UMPS scores, this association indicates that these facial changes are signs of acute pain in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105734"},"PeriodicalIF":2.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of concern: \"Impact of Cryptosporidium parvum infection on glucose transport and metabolism in calves: Physiological and molecular insight\" [Research in Veterinary Science (2024), In press].","authors":"","doi":"10.1016/j.rvsc.2025.105566","DOIUrl":"https://doi.org/10.1016/j.rvsc.2025.105566","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"189 ","pages":"105566"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LiDSCUNet++: A lightweight depth separable convolutional UNet++ for vertebral column segmentation and spondylosis detection","authors":"Krishna K. Agrawal,&nbsp;Gautam Kumar","doi":"10.1016/j.rvsc.2025.105703","DOIUrl":"10.1016/j.rvsc.2025.105703","url":null,"abstract":"<div><div>Accurate computer-aided diagnosis systems rely on precise segmentation of the vertebral column to assist physicians in diagnosing various disorders. However, segmenting spinal disks and bones becomes challenging in the presence of abnormalities and complex anatomical structures. While Deep Convolutional Neural Networks (DCNNs) achieve remarkable results in medical image segmentation, their performance is limited by data insufficiency and the high computational complexity of existing solutions. This paper introduces LiDSCUNet++, a lightweight deep learning framework based on depthwise-separable and pointwise convolutions integrated with UNet++ for vertebral column segmentation. The model segments vertebral anomalies from dog radiographs, and the results are further processed by YOLOv8 for automated detection of Spondylosis Deformans. LiDSCUNet++ delivers comparable segmentation performance while significantly reducing trainable parameters, memory usage, energy consumption, and computational time, making it an efficient and practical solution for medical image analysis.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105703"},"PeriodicalIF":2.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γδ T cells induced by zoledronate under macrophage-depleted conditions reduce disease severity and parasite number in Eimeria tenella-infected chicks","authors":"Quan Viet Le ,&nbsp;Makoto Matsubayashi ,&nbsp;Toshimitsu Hatabu","doi":"10.1016/j.rvsc.2025.105730","DOIUrl":"10.1016/j.rvsc.2025.105730","url":null,"abstract":"<div><div>Disease severity and local immune responses mediated by Zoledronate (ZOL)-induced γδ T cells were evaluated in chicks infected with <em>E. tenella</em> under macrophage-depleted conditions. Three groups of White Leghorns, including a negative control group (CT), carrageenan-treated group (CAR), and both ZOL and CAR-treated group (ZOL/CAR), were infected orally with <em>E. tenella</em> sporulated oocysts (1 × 10⁴ oocysts/chick) at 14 days of age. Fecal oocyst shedding was assessed at 4–15 days post-infection. The cecum was collected for histopathological and gene expression analyses. Oocyst shedding was significantly reduced in the CAR and ZOL/CAR groups compared with the CT group (<em>p</em> &lt; 0.01). The total oocyst number in the ZOL/CAR group was lower compared with those in the CAR and CT groups (<em>p</em> &lt; 0.05). The lesion score and parasite burden in the CAR and ZOL/CAR groups were lower compared with those in the CT group (<em>p</em> &lt; 0.01). The expression of IFN-λ, IL-17 A, and perforin mRNA in the ZOL/CAR group increased compared with that in the CAR group, whereas the expression of IFN-γ in the ZOL/CAR group decreased. The results suggest that Th1-independent immunity occurs as a local immune response induced by IL-17 A/IL-22-producing γδ T cells, which control <em>E. tenella</em>-induced pathology in the cecum.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105730"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale serosurvey of Chlamydia abortus in zoo ungulates in Spain","authors":"Adrián Beato-Benítez ,&nbsp;Ignacio García-Bocanegra ,&nbsp;Moisés Gonzálvez ,&nbsp;Jessica Villar-Ibáñez ,&nbsp;Débora Jiménez-Martín ,&nbsp;Rosa Martínez-Valverde ,&nbsp;Santiago Borragán ,&nbsp;Manuel de la Riva-Fraga ,&nbsp;David Cano-Terriza","doi":"10.1016/j.rvsc.2025.105731","DOIUrl":"10.1016/j.rvsc.2025.105731","url":null,"abstract":"<div><div><em>Chlamydia abortus</em> is a zoonotic bacterium worldwide distributed, which mainly leads to reproductive disorders in different domestic and wild species. Although <em>C. abortus</em> infection has been widely reported in free-ranging ungulates, information regarding this pathogen in captive wild ungulates remains limited. The aims of the present study were to assess <em>C. abortus</em> exposure in zoo ungulates in Spain and to determine the dynamics of seropositivity in animals sampled longitudinally throughout the study period. Between 2007 and 2023, serum samples from 483 ungulates representing 65 species were collected across 11 zoos from Spain. Additionally, 42 of the 483 animals were longitudinally sampled. Anti-<em>C. abortus</em> antibodies were detected in ten individuals (2.1 %; 95 %CI: 0.8–3.3) using a commercial indirect multi-species ELISA. Seropositivity was found in 9.2 % (6/65) of the species analyzed and in 45.5 % (5/11) of the zoos sampled. Seroprevalence was significantly higher during the period 2019–2020 compared to 2007–2014, 2015–2018 and 2021–2023 (<em>P</em> = 0.013). Three of the 42 longitudinally surveyed animals presented seropositivity throughout the study period. This is the first large-scale serosurvey of <em>C. abortus</em> conducted in zoo ungulates, also describing for the first time exposure to <em>C. abortus</em> in four species, which could expand the host range of this zoonotic bacterium. Our results suggest a limited but widespread circulation of <em>C. abortus</em> in wild ungulates housed in zoos in Spain. Zoo ungulates should be included in surveillance programs for <em>C. abortus</em> to enhance our understanding of the epidemiology of this zoonotic pathogen in anthropized environments under a One Health framework.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105731"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}