Research in veterinary science最新文献

{"title":"Untargeted analysis of the plasma metabolome in canine acute pancreatitis","authors":"Piyada Na Nakorn ,&nbsp;Duangthip Chatchaisak ,&nbsp;Boonrat Chantong ,&nbsp;Supitcha Pannengpetch ,&nbsp;Sasiwimon Lalitmanat ,&nbsp;Weerapat Chawanlawuthi ,&nbsp;Sataporn Phochantachinda","doi":"10.1016/j.rvsc.2025.105900","DOIUrl":"10.1016/j.rvsc.2025.105900","url":null,"abstract":"<div><div>Acute pancreatitis is a common condition in dogs associated with significant diagnostic and management challenges. Although current treatments largely provide supportive and symptomatic care, a more profound understanding of the metabolic response of canine acute pancreatitis might facilitate the development of more targeted interventions. This study characterized the plasma metabolomic profile of dogs with acute pancreatitis. Twelve dogs, including six clinically diagnosed with acute pancreatitis and six healthy controls, were investigated. Plasma samples were analyzed using liquid chromatography–tandem mass spectrometry, and the resulting metabolomic data were assessed using partial least squares discriminant analysis, revealing clear separation between the groups. Pathway analysis identified significant alterations in glycine, serine, and threonine metabolism; histidine metabolism; and arginine biosynthesis, with additional trends observed in nitrogen metabolism. Five metabolites displayed diagnostic relevance, with γ-glutamylmethionine, 4-hydroxynonenal–glutathione, γ-glutamylalanine, and pyrocatechol levels being significantly reduced in dogs with acute pancreatitis (all <em>p</em> &lt; 0.001) and <em>N</em>-acetyl-Leu levels being significantly elevated (<em>p</em> &lt; 0.001). These metabolites demonstrated strong discriminatory power, and they represent potential biomarkers for canine acute pancreatitis, particularly reflecting oxidative stress, inflammation, and altered amino acid metabolism. Further research is warranted to validate their clinical utility and explore their mechanistic roles in the pathophysiology of acute pancreatitis, potentially guiding future diagnostic and therapeutic innovations in veterinary medicine.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105900"},"PeriodicalIF":1.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance, sequence typing, and virulence gene profiles of Salmonella enterica isolated from the broiler production chain in southern Thailand","authors":"Bussara Sripaurya ,&nbsp;Wattana Pelyuntha ,&nbsp;Ruttayaporn Ngasaman ,&nbsp;Chai Lay Ching ,&nbsp;Vincent Guyonnet ,&nbsp;Kitiya Vongkamjan","doi":"10.1016/j.rvsc.2025.105891","DOIUrl":"10.1016/j.rvsc.2025.105891","url":null,"abstract":"<div><div><em>Salmonella</em> is a major foodborne pathogen from poultry farms and potentially transferred to meat products during processing in slaughterhouses or transportation to wet markets. From a total of 351 samples, only 73 samples (20.8 %) collected from broiler farms, slaughterhouses, and chicken meat stalls at a wet market, which were selected independently within the same province, were positive for <em>Salmonella</em>. Fifty-six <em>Salmonella</em> isolates were evaluated for the presence of nine known virulence genes (<em>sipB, prgH, spaN, orgA, tolC, sitC, sifA, cdtB,</em> and <em>sopB</em>) and their antibiotic resistance profiles. Pattern II (<em>cdt</em>B negative) represented the most predominant pattern found in 23 <em>Salmonella</em> isolates. The most common antibiotic resistance profile was AMP-TE-CIP-NA, found in 10 isolates from all sources. The relationship of subtypes of <em>Salmonella</em> spp. isolated, analyzed by multilocus sequence typing (MLST), revealed twelve sequence types (STs). The most frequently detected sequence type was ST198 (26.8 %), identified in <em>S.</em> Kentucky. In this study, ST198 was identified in six multidrug resistance (MDR) profiles from all sources and was associated with ampicillin and ciprofloxacin resistance. Overall, our study indicated the potential public health benefits of monitoring STs and antibiotic resistance phenotypes to track the spread of MDR in <em>Salmonella</em> isolates from specific sources, including within the broiler value chain.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105891"},"PeriodicalIF":1.8,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning enhanced thermographic modeling for early and precise mastitis detection in Sahiwal cows","authors":"S.L. Gayathri ,&nbsp;M. Bhakat ,&nbsp;T.K. Mohanty ,&nbsp;R.R. Kumar ,&nbsp;K.K. Chaturvedi ,&nbsp;S. Kumar","doi":"10.1016/j.rvsc.2025.105899","DOIUrl":"10.1016/j.rvsc.2025.105899","url":null,"abstract":"<div><div>Mastitis, a multifactorial production disease, poses a significant challenge to dairy farming, necessitating the adoption of integrated and precision-based diagnostic approaches. This study explores the potential of thermal imaging combined with deep learning to enhance mastitis detection in lactating dairy cows. In this study, thermal images of the udder region of Sahiwal cows were captured using a handheld thermal camera and analyzed to classify udder quarters as healthy, Sub-clinical Mastitis (SCM), and Clinical Mastitis (CM). The classification was based on California Mastitis Test (CMT) scores, Somatic Cell Count (SCC) values, and thermal image analysis. Further, Convolutional Neural Network (CNN) models were developed to distinguish between healthy udder quarters and those affected by CM or SCM. The CNN model differentiating healthy quarters from CM achieved training, validation, and testing accuracies of 99 %, with precision, recall, and F1-score all at 0.99. Similarly, the model distinguishing healthy quarters from SCM recorded training and validation accuracies of 89 % and 85 %, respectively, while testing results showed an accuracy of 84 %, a precision of 0.87, a recall of 0.79, and an F1-score of 0.83. These findings highlight the potential of CNN-based thermal imaging for accurate mastitis detection, contributing to advancements in precision dairy farming and livestock health management.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105899"},"PeriodicalIF":1.8,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study of synovial fluid-derived mesenchymal stromal cell therapy for canine osteoarthritis","authors":"Yasuhiro Koga ,&nbsp;Makoto Fujiki ,&nbsp;Tatsuro Hifumi ,&nbsp;Erisa Moriwaki ,&nbsp;Shunmei Chiba ,&nbsp;Kazuhiro Misumi","doi":"10.1016/j.rvsc.2025.105897","DOIUrl":"10.1016/j.rvsc.2025.105897","url":null,"abstract":"<div><div>Dogs with osteoarthritis (OA) may benefit from stromal cell therapies, but there is a lack of such established therapies. Accordingly, we investigated the feasibility and safety of synovial fluid (SF)-derived mesenchymal stromal cell (MSC) transplantation for canine OA. MSCs were isolated from SF (<em>n</em> = 15) or by joint lavage (JLF; <em>n</em> = 12) from the knee joints of dogs undergoing orthopedic surgery (<em>n</em> = 25), and then cultured on a dish (passage 1) and a polyethylene terephthalate (PET) nonwoven fabric scaffold (passage 2). We obtained 2166 ± 1177 (SF) or 1640 ± 1064 (JLF) × 10⁴ primary cells and 18,673 ± 8500 (SF) or 16,870 ± 3348 (JLF) × 10⁴ passaged cells, surpassing the target number. Passaged cells were morphologically uniform, self-proliferating, CD29, CD44, CD90, and CD105-positive, and CD31-negative cells, with the ability to differentiate into osteoblasts, chondrocytes, and adipocytes. Healthy dogs (<em>n</em> = 3) then received a 1-mL allogenic MSC suspension (at 1×, 5×, and 10 × 10⁷ cells), harvested and prepared with the same method, by intraarticular injection (with contralateral knees as negative controls), and were evaluated for adverse clinical signs, hematologic and biochemical parameters periodically, and joint images and pathology at day 90. No abnormal findings were found in any dogs, confirming the safety of allogeneic MSC transplantation</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105897"},"PeriodicalIF":1.8,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitological, serological and molecular evaluation of Trypanosoma vivax in cattle experimentally infected and early treated with isometamidium chloride in a region lacking the biological vector","authors":"Haryie Victória Iuasse ,&nbsp;Vanessa Ferreira Salvador ,&nbsp;Igor Maciel Lopes de Morais ,&nbsp;Luccas Lourenzzo Lima Lins Leal ,&nbsp;Luiz Fellipe Monteiro Couto ,&nbsp;Artur Siqueira Nunes Trindade ,&nbsp;Gabriel Lopes Tamiozo ,&nbsp;Lídia Mendes de Aquino ,&nbsp;Thiago Souza Azeredo Bastos ,&nbsp;Rosangela Zacarias Machado ,&nbsp;Lorena Lopes Ferreira ,&nbsp;Fabiano Antônio Cadioli ,&nbsp;Welber Daniel Zanetti Lopes","doi":"10.1016/j.rvsc.2025.105898","DOIUrl":"10.1016/j.rvsc.2025.105898","url":null,"abstract":"<div><div>This study aimed to evaluate whether early treatment with isometamidium chloride (ISM) affects the production of anti-<em>Trypanosoma vivax</em> antibodies in cattle and whether it can induce parasitological cure, based on tissue analysis 546 days post-treatment in animals without evidence of parasitological relapse. Ten male cattle were experimentally infected with ≈ 1 × 10⁶ viable <em>T. vivax</em> trypomastigotes (D-6) and treated with ISM six days post-infection (D0). The animals were euthanized 546 days post-treatment. During this period, the presence of <em>T. vivax</em> in blood was assessed using direct methods (Woo, Brener, and conventional PCR – cPCR) and serological analysis (indirect iELISA - iELISA). Liver, spleen, brain, heart and pre-scapular lymph node were collected and examined using cPCR. Molecular analysis of blood detected <em>T. vivax</em> DNA up to the day of treatment, but none thereafter. No <em>T. vivax</em> relapses in bloodstream was detected. Anti-<em>T. vivax</em> antibodies were detected from 42 to 546 days post-treatment, except in one animal. However, <em>T. vivax</em> DNA was still detected in the liver, brain, heart, and spleen of at least one animal at 546 days post-treatment. These findings suggest that early ISM treatment does not achieve complete parasitological clearance, as both antibodies and parasite DNA were detected.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105898"},"PeriodicalIF":1.8,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraosseous adipose mesenchymal stem cells and plasma rich in growth factors: A promising approach for articular cartilage and subchondral bone regeneration","authors":"Marta Torres-Torrillas ,&nbsp;Elena Damiá ,&nbsp;Pau Peláez ,&nbsp;Ayla del Romero ,&nbsp;Laura Miguel-Pastor ,&nbsp;Mónica Rubio ,&nbsp;Belén Cuervo ,&nbsp;José M. Carrillo ,&nbsp;Joaquín J. Sopena","doi":"10.1016/j.rvsc.2025.105896","DOIUrl":"10.1016/j.rvsc.2025.105896","url":null,"abstract":"<div><div>Intra-articular (IA) infiltration of plasma rich in growth factors (PRGF) and adipose-derived mesenchymal stem cells (ASCs) have been proposed as a potential treatment for patients with osteoarthritis, due to their regenerative properties and ability to promote cartilage healing. However, in patients with severe osteoarthritis, where not only cartilage but also the subchondral bone is affected, IA infiltration of these regenerative therapies appears to be less effective. To overcome this limitation, intraosseous (IO) infiltration of PRGF and ASCs has recently been proposed. The objective of this study was to evaluate the effectiveness of IO infiltration with PRGF, ASCs, and their combination (PRGF+ASCs) in treating acute full-thickness chondral lesions in a rabbit model. Forty healthy rabbits were allocated into four groups based on the IO treatment administered (control, PRGF, ASCs, or PRGF+ASCs). A full-thickness chondral defect was created in the medial femoral condyle, followed by IO treatment infiltration. Additionally, all rabbits, regardless of group, received an IA infiltration of PRGF. At 56- and 84-days post-infiltration, the groups treated with any of the three regenerative therapies showed improved histological outcomes compared to the control group, with the PRGF+ASCs group demonstrating the most significant cartilage healing. The results suggest that IO infiltration of PRGF, ASCs, or their combination enhances the regeneration of articular cartilage and subchondral bone and provides more sustained effects compared to IA infiltration of PRGF alone.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105896"},"PeriodicalIF":1.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niclosamide modulates chronic Cryptosporidium-induced ileocecal adenocarcinoma in immunocompromised infected mice via targeting IL-6/IL-22-STAT3 axis","authors":"Eman S. El-Wakil ,&nbsp;Walaa H. El-Maadawy ,&nbsp;Ahmed A. Bayomy ,&nbsp;Mohammed Y. Shakra ,&nbsp;Ahmed M.A. Mohamed ,&nbsp;Mostafa El shahat Mostafa ,&nbsp;Hajar S. Helal","doi":"10.1016/j.rvsc.2025.105895","DOIUrl":"10.1016/j.rvsc.2025.105895","url":null,"abstract":"<div><div>Cryptosporidiosis, a protozoal disease caused by <em>Cryptosporidium parvum</em> (<em>C. parvum</em>), is a globally emerging waterborne infection leading to moderate to severe diarrhea, particularly in immunocompromised individuals. Emerging evidence proposes a potential correlation between chronic infection and digestive cancers, though the underlying mechanisms remain unclear. Therapeutic options are minimal, with nitazoxanide (NTZ) being the only drug available, despite its constrained effectiveness. Niclosamide (NICLO), an anthelmintic drug, exhibits antiparasitic properties and modulates oncogenic signaling pathways, making it a promising candidate for dual therapeutic effects. This study investigated NICLO's efficacy against chronic <em>C. parvum</em> infection and its potential to prevent ileocecal adenocarcinoma development in immunocompromised mice. Mice were immunosuppressed using dexamethasone, infected with <em>C. parvum</em> oocysts, and administered NTZ or NICLO. NICLO administration significantly reduced parasite burden by 90 %, restored histopathological alterations, and suppressed <em>C. parvum</em>-induced inflammation, as evidenced by suppressed IL-6 and IL-22 levels and STAT3 activation. NICLO also counteracted the antiapoptotic effects of infection by downregulating Bcl-2 and upregulating Bax and cleaved caspase-3 levels. Moreover, NICLO deactivated the Wnt/β-catenin pathway and its target genes (survivin, c-myc, and cyclin D) while elevating p53 levels. These findings highlight NICLO's dual therapeutic potential, which effectively targets <em>C. parvum</em> infection and mitigates its tumorigenic effects by disrupting key signaling pathways involved in ileocecal cancer progression. This study provides novel insights into NICLO's chemotherapeutic efficacy in managing cryptosporidiosis and its associated oncogenic potential.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105895"},"PeriodicalIF":1.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Lactobacillus supplementation on seizure control, gut microbiota, and blood neurotransmitters in dogs with idiopathic epilepsy","authors":"Sylvia García-Belenguer ,&nbsp;Belén Rosado ,&nbsp;Adelaida Hernaiz ,&nbsp;Jon Moral ,&nbsp;Inmaculada Martín-Burriel ,&nbsp;Jorge Palacio","doi":"10.1016/j.rvsc.2025.105888","DOIUrl":"10.1016/j.rvsc.2025.105888","url":null,"abstract":"<div><div>Idiopathic epilepsy is one of the most common neurological diseases in dogs; however, epileptogenesis and the mechanisms underlying drug resistance remain poorly understood. This study aimed to evaluate the effects of three months of supplementation with canine-derived <em>Lactobacillaceae</em> strains on seizure frequency in dogs with drug-sensitive epilepsy (DSE) and drug-refractory epilepsy (DRE). Additionally, gut microbiota profiles and plasma levels of GABA, L-glutamate, and serotonin were analysed and compared to those of healthy control dogs.</div><div>The probiotic supplementation was associated with a reduction in both the number of seizures and seizure days in epileptic dogs. An increase in the relative abundance of gut <em>Lactobacillus</em> and plasma GABA levels did not accompany clinical improvement. However, it was associated with a significant decrease in the GABA/L-Glutamate ratio in dogs with DSE but not in those with DRE. At baseline, heterogeneity of the fecal microbial community (β-diversity) was higher in epileptic compared to controls. Following probiotic supplementation, the differences in β-diversity between control and DSE dogs were no longer observed. The probiotic had no notable effect on the control group but was associated with a significant reduction in β-diversity in dogs with DRE.</div><div>While the relative abundance of <em>Lactobacillus</em> remained unchanged after probiotic administration, some changes in gut microbiota were observed in epileptic dogs. Numerous associations were identified between seizure frequency and specific bacterial taxa with potential protective effects (e.g.<em>,Prevotella_9, Ligilactobacillus</em>) or risk effects (e.g.<em>,Parasutterella, Helicobacter</em>). These findings suggest that long-term use of <em>Lactobacillus</em> may serve as a promising adjuvant strategy for seizure control.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105888"},"PeriodicalIF":1.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of a new muscle cell line developed from pearl spot, Etroplus suratensis (Bloch 1790)","authors":"M. Goswami ,&nbsp;Gowhar Iqbal ,&nbsp;Nevil Pinto ,&nbsp;Darshan Pawaskar,&nbsp;Kiran D. Rasal,&nbsp;A. Sathiyanarayanan","doi":"10.1016/j.rvsc.2025.105880","DOIUrl":"10.1016/j.rvsc.2025.105880","url":null,"abstract":"<div><div>Cell lines originating from diverse fish species inhabiting freshwater, brackish water, and marine environments have emerged as an innovative <em>in vitro</em> research tool in bioscience. A new cell line was developed from the muscle tissue of <em>Etroplus suratensis</em> (Pearl spot). The primary muscle cell culture system was successfully obtained from the dorsal muscle tissue was used for explant preparation of <em>Etroplus suratensis</em> and subsequently subcultured up to 15 passages, resulting in the development of a novel muscle cell line termed ESM (<em>Etroplus suratensis</em> muscle). Initially, the ESM cells were cultured in Leibovitz's 15 media (L-15) supplemented with 20 % fetal bovine serum (FBS) at a temperature of 28 °C. Muscle cells were then optimized for growth in culture media by reducing the FBS concentration to 15 % FBS and 5 % FBS. Additionally, basic fibroblast growth factor (bFGF), at a concentration of 10 ng/mL, was added to the cell culture flask so that the proliferation capacity of the ESM cell line could be increased and also reduce the time interval of subculture at different passages. Immunocytochemistry was carried out to understand the expression of myogenic regulatory factors MyoD and Pax7 in the developed cell line. The transfection efficiency of the ESM cells was 9 % using the pMaxGFP vector. The cytotoxicity assay of three heavy metal salts (Cd, Cu, Pb) was assessed in ESM cells using XTT and MTT assays. The COI gene was used to authenticate the species of origin of the developed ESM cell line. The ESM cell line was cryopreserved in liquid nitrogen (−196 °C). This is the first report of a muscle cell line derived from the fish species <em>Etroplus suratensis.</em></div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105880"},"PeriodicalIF":1.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance and genetic characterization of infectious pancreatic necrosis virus in rainbow trout farms of East Azerbaijan, Iran","authors":"Amin Reza Makhdoum ,&nbsp;Amin Gholamhosseini ,&nbsp;Mohammadreza Ghorani ,&nbsp;Vahideh Taghadosi ,&nbsp;Nasser Hajipour","doi":"10.1016/j.rvsc.2025.105894","DOIUrl":"10.1016/j.rvsc.2025.105894","url":null,"abstract":"<div><div>Infectious pancreatic necrosis virus (IPNV), a member of the <em>Birnaviridae</em> family, is a significant pathogen in salmonid aquaculture, causing notable economic losses worldwide. This study aimed to detect and genetically characterize IPNV in rainbow trout (<em>Oncorhynchus mykiss</em>) farms in East Azerbaijan Province, Iran. Sampling was conducted at 12 farms. Five tissue samples were taken from each farm. A total of 60 tissue samples from juvenile fish (&lt;10 g) were collected across 12 farms. RNA was extracted and subjected to nested reverse transcription polymerase chain reaction (RT-PCR) targeting the NS/VP3 region in segment A of the IPNV genome. The virus was detected in 8 out of 60 samples (13.3 %), with positive cases identified in three farms. Sequencing was performed on four positive samples. Sequence analysis of four representative isolates (PP316090, PP316091, PP429499, PP429500) revealed high nucleotide similarity to previously reported Iranian strains from Shiraz and Ahvaz, as well as European strains, particularly from Spain and Turkey. Phylogenetic analysis confirmed close clustering with endemic Iranian isolates, indicating minimal genetic variation and possible intra-regional transmission. The persistence of genetically stable strains over time suggests that current biosecurity measures may be insufficient to prevent viral spread. These results highlight the need for improved monitoring systems, targeted molecular diagnostics, and the development of region-specific vaccines to manage IPNV effectively. Furthermore, the genetic resemblance to European isolates highlights the importance of broader epidemiological studies. Continued molecular surveillance and strain tracking are essential for early detection and control. Future studies should also explore the pathogenic potential of local strains and assess their impact on fish health and farm productivity under field conditions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105894"},"PeriodicalIF":1.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}