Research in veterinary science最新文献

{"title":"Exploring endosymbionts and pathogens in Rhipicephalus sanguineus and Ctenocephalides felis felis with Oxford Nanopore Technology","authors":"Luisa Páez-Triana ,&nbsp;David Martinez ,&nbsp;Luz H. Patiño ,&nbsp;Marina Muñoz ,&nbsp;Claudia M. Sandoval-Ramírez ,&nbsp;Juan Carlos Pinilla León ,&nbsp;Juan David Ramirez","doi":"10.1016/j.rvsc.2025.105562","DOIUrl":"10.1016/j.rvsc.2025.105562","url":null,"abstract":"<div><div>Fleas and ticks play a crucial role in public health as vectors of multiple diseases affecting humans and animals. Several rickettsial pathogens and endosymbionts are transmitted by fleas and ticks. Therefore, understanding this group of microorganisms is essential for fully grasping the spectrum of pathogens transmitted by vectors and the interactions between endosymbiotic microorganisms and their hosts. This study evaluated the presence and diversity of <em>Rickettsiales</em> species in fleas and ticks collected from the Santander department in Colombia. For the methodology a 16S gene amplification approach through Oxford Nanopore sequencing technologies in <em>Rhipicephalus sanguineus</em> and <em>Ctenocephalides felis felis</em> was used. Our findings revealed the presence of multiple pathogenic and endosymbiotic microorganisms, particularly from the <em>Rickettsia</em> and <em>Wolbachia</em> groups. We observed a clear association between <em>Rickettsia</em> species and ticks, while <em>Wolbachia</em> was predominantly found in fleas. Additionally, other important microorganisms were identified, including <em>Anaplasma phagocytophilum</em>, <em>Rickettsia conorii</em>, and different strains of <em>Wolbachia</em> that serve as endosymbionts in various arthropods. These results underscore the importance of fleas and ticks in the transmission of both pathogenic and endosymbiotic microorganisms. The distinct patterns of association between specific pathogens and vectors provide insight into their transmission dynamics. Identifying pathogens such as <em>Anaplasma phagocytophilum</em> and <em>Rickettsia conorii</em> further highlights the need for continued research into vector-borne diseases in Colombia. Understanding the interactions between endosymbionts and pathogenic microorganisms in these vectors could lead to the development of more effective strategies for controlling diseases transmitted by fleas and ticks.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105562"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143337940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Enterobacteriaceae from dairy cattle milk: A systematic review and meta-analysis","authors":"Dian Meididewi Nuraini ,&nbsp;Morsid Andityas ,&nbsp;Peerapol Sukon ,&nbsp;Patchara Phuektes","doi":"10.1016/j.rvsc.2024.105497","DOIUrl":"10.1016/j.rvsc.2024.105497","url":null,"abstract":"<div><div>Carbapenem-resistant <em>Enterobacteriaceae</em> (CRE) have been detected in dairy cattle milk, raising concerns about public health risks. This study aimed to assess the global prevalence of CRE in dairy cattle milk through a systematic review and meta-analysis, following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Retrieved articles from four databases were initially screened based on predefined inclusion criteria. The meta-analysis included 49 studies (2011–2024), covering 28,134 milk samples and 3462 <em>Enterobacteriaceae</em> isolates globally. Data from the full text were extracted to a Microsoft Excel spreadsheet and analysed using the ‘meta’ R package in R v.4.3.0 software for pooled prevalence and subgroup meta-analysis with a random-effects for logit transformation. Heterogeneity was assessed using Cochran's Q statistic (χ²), <em>p</em>-value and <em>I</em>^<em>2</em> statistic. Publication bias and sensitivity were evaluated using Egger's test, funnel plot, trim and fill plot, and leave-one-out test. Globally, the prevalence of CRE in dairy cattle milk was 0.73 % (95 % CI, 0.37–1.41). Subgroup meta-analysis based on continent, sample type, <em>Enterobacteriaceae</em> species, diagnostic method, antibiotic type, and interpretation guideline revealed no significant differences among the criteria within the subgroup. Although the overall pooled prevalence of CRE in dairy cattle milk is relatively low, it raises public health concern regarding raw milk consumption. This emphasizes the need for regular monitoring with in a One Health framework for CRE in the dairy industry to anticipate potential transmission between humans, animals, and the environment.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105497"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of delayed milk ejection on mammary gland health and milking performance in dairy cows: A systematic review and meta-analysis","authors":"Mohammad Dahl ,&nbsp;Matthias Wieland","doi":"10.1016/j.rvsc.2024.105510","DOIUrl":"10.1016/j.rvsc.2024.105510","url":null,"abstract":"<div><div>Delayed milk ejection (DME) could compromise udder health and milking performance. The objectives of this systematic review and meta-analysis were to (i) explore the current evidence, assess the risk of bias, and identify the knowledge gaps in published studies that investigated the effect of DME on udder health and milking performance in dairy cows, and (ii) quantify the magnitude of the difference in milk yield per milking session (MY), milking duration (MD), and peak milk flow rate (PFR) between cows with and without DME. We performed the current systematic review and meta-analysis according to PRISMA 2020 statement. We tracked the pertinent peer-reviewed publications from PubMed using the following syntax (delayed milk ejection OR disturbed milk ejection OR bimodal milk flow OR bimodality) AND (bovine OR cattle OR cows), and additional relevant studies from the identified articles' reference lists. We calculated the pooled-mean difference in MY (kg), MD (seconds), and PFR (kg/min) between cows with and without DME using the random-effects models. The selection process identified 15 studies as the final number of studies qualified for review, including six interventional and nine observational studies published between 1980 and 2023 conducted in seven countries, including the USA, Germany, Italy, Switzerland, Netherlands, New Zealand, and Portugal. The results indicated that while the DME had a minimal influence on the udder health, it can reduce MY by 1.55 kg per milking session (95 % CI = −2.10, −1.00). The observed evidence here suggests that DME can negatively influence the milking profitability.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105510"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of prevalence, risk factors, and therapeutic approach for subclinical endometritis and oviductal occlusion in repeat breeder Holstein cattle","authors":"Sofía L. Villar ,&nbsp;Carlos C. Pérez-Marín ,&nbsp;Jacobo Álvarez ,&nbsp;Uxía Yáñez ,&nbsp;Juan J. Becerra ,&nbsp;Ana I. Peña ,&nbsp;Pedro G. Herradón ,&nbsp;Luis A. Quintela","doi":"10.1016/j.rvsc.2024.105511","DOIUrl":"10.1016/j.rvsc.2024.105511","url":null,"abstract":"<div><div>Due to the productive and economic consequences of Repeat Breeder (RB) syndrome, the objectives of this study were to determine the prevalence and risk factors for subclinical endometritis (SE) and oviductal occlusion (OO) in RB cows, and to make a therapeutic approach for these pathologies. In 99 RB cows, endometrial cytologies were performed to assess the presence of SE (&gt;5 % polymorphonuclear neutrophils), and the oviductal patency was checked using the phenolsulfonphthalein test. Body condition score was evaluated, and data from each animal were obtained from on-farm software (parity, calving date, artificial insemination (AI) date, number of AI, and occurrence of postpartum diseases). Cows positive to SE were assigned to one treatment protocol: a) Non-steroidal anti-inflammatory drug (NSAID), b) Prostaglandin F_2α (PGF_2α), c) NSAID+ PGF_2α. Similarly, cows with OO were assigned to one treatment: a) Therapeutic embryo, b) Artificial insemination. The prevalence for SE and OO was 22 % and 3 %, respectively. Regarding SE, the logistic regression did not show any significant difference for the risk factors evaluated. Moreover, cows that were administered the NSAID, either alone or in combination with PGF_2α, showed higher conception rates in the following AI (<em>p</em> &lt; 0.05). Due to the low prevalence of OO, it was not possible to perform the logistic regression analysis. In conclusion, neither SE nor OO seem to be the main cause of RB syndrome in the cows under study. Additionally, when SE is diagnosed as the main cause of RB syndrome, treatment with NAISDs seems to increase conception rates.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105511"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary steps for fabrication of microfluidic systems for swine sperm sorting: Materials, perfusing systems and flow","authors":"A. Lacueva-Aparicio ,&nbsp;R. Monge ,&nbsp;L. Serrano ,&nbsp;C. Malo","doi":"10.1016/j.rvsc.2024.105488","DOIUrl":"10.1016/j.rvsc.2024.105488","url":null,"abstract":"<div><div>The success rate of assisted reproductive techniques in the livestock production can be optimized by improving the quality of the semen sample by selecting only the good quality sperm from the ejaculate. Microfluidic technology has been studied for sperm sorting mainly in human ejaculates but has not been studied for boar sperm. Spermatozoa have been proven to be highly sensitive to different microplastics, but the potential toxic effects of the materials used to set up microfluidic systems have not been studied. The main goal of this study was to assess the possible toxic effect on boar sperm of materials commonly used for a microfluidic system and to evaluate the effect of different flow control systems (peristaltic pump, syringe pump and a microfluidic flow controller) at different flow rates (10 μl*min-1, 100 μl*min-1 and 1 ml*min-1) on sperm quality, as preliminary information for the development of a swine sperm sorting microfluidic system. Results showed no negative effect of the different materials at different concentrations. The control reached the highest curvilinear velocity compared to the peristaltic pump and the pressure-based flow control system. In the flow rates, 10 μm*min-1 showed the poorest results and no significant differences were observed between control and 1 mlmin-1 flow in any of the parameters. In conclusion, all materials that were studied for microfluidic fabrications were suitable for sperm sorting, any of the pumps would be suitable for sperm selection and 1 ml*min-1 flow rate would be the flow rate of choice for sperm pumping.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105488"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct amino acid substitutions in the EEV glycoprotein and DNA-dependent RNA polymerase of lumpy skin disease virus identified in wetland areas of Bangladesh","authors":"Moslema Jahan Mou ,&nbsp;S.M. Nazmul Hasan ,&nbsp;Anandha Mozumder ,&nbsp;Marjana Akter ,&nbsp;Riyan Al Islam Reshad ,&nbsp;Roni Mia ,&nbsp;Md. Salauddin ,&nbsp;M. Shaminur Rahman ,&nbsp;Md. Mahmudul Alam ,&nbsp;Sharmin Akter ,&nbsp;Sukumar Saha ,&nbsp;Tofazzal Islam ,&nbsp;Md. Golzar Hossain","doi":"10.1016/j.rvsc.2024.105508","DOIUrl":"10.1016/j.rvsc.2024.105508","url":null,"abstract":"<div><div>The recent outbreak of lumpy skin disease virus (LSDV) in the wetland areas of Bangladesh presents a significant concern for both animal health and regional biosecurity. Epidemiological investigations into nine major outbreaks in the wetland areas revealed distinctive clinical symptoms in affected cattle, including elevated body temperature, excessive salivation, and the presence of skin nodules. Histopathological examination unveiled larger nodules compared to previous outbreaks, along with signs of secondary infection. Molecular analysis confirmed the presence of LSDV in all samples, with subsequent sequencing revealing genetic similarities with virus isolates of Bangladesh, India, China, Russia, Serbia and Greece. Most importantly amino acid variations in the viral EEV glycoprotein and DNA-dependent RNA polymerase were revealed that also altered the structures of the respective proteins significantly suggesting potential implications for viral pathogenesis. Additionally, successful isolation of LSDV in Vero cells demonstrated cytopathic effects, supporting the potential for vaccine development. In conclusion, this study provides comprehensive insights into the epidemiology, genomic characters with altered predicted structures of two major viral proteins and pathogenesis of LSDV outbreaks in Bangladesh. These findings emphasize the critical need for ongoing monitoring and adaptive control strategies, including the development of effective vaccines, to mitigate the impact of LSDV in affected regions and safeguard regional livestock health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105508"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel hydroxy naphthol blue-based recombinase polymerase amplification for the rapid detection of Staphylococcus pseudintermedius","authors":"Pavarish Jantorn ,&nbsp;Aekkaraj Nualla-ong ,&nbsp;Dennapa Saeloh Sotthibandhu","doi":"10.1016/j.rvsc.2024.105520","DOIUrl":"10.1016/j.rvsc.2024.105520","url":null,"abstract":"<div><div><em>Staphylococcus pseudintermedius</em> (<em>S. pseudintermedius</em>) is a significant cause of pyoderma, soft tissue, urinary tract, and ear infections in cats and dogs. Bacterial culture and biochemical phenotypic assays are the gold standards for clinical diagnosis of bacteria but molecular methods have now been developed to identify and differentiate <em>S. pseudintermedius</em>. However, these methods require complex and expensive equipment, restricting usage in many laboratories. This study developed a rapid, specific, and sensitive detection method for <em>S. pseudintermedius</em> based on recombinase polymerase amplification (RPA) combined with hydroxy naphthol blue (HNB) dye. The RPA primer was designed, optimized, and subjected to amplify the <em>sps</em>L gene as the molecular diagnostic target. HNB dye was added to the reaction, successfully enabling the development of a colorimetric detection method for <em>S. pseudintermedius.</em> This RPA-HNB assay completed amplification at 37 °C within 20 min, with results visualized by the naked eye as a color change. All processes were completed within an hour. The assay showed the limit of detection at 1 copy per reaction, with sensitivity and specificity against <em>S. pseudintermedius</em> 0.98 and 1.00, respectively. Results indicated that our assay provided high sensitivity and specificity for detecting <em>S. pseudintermedius</em> with reduced detection time.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105520"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating compliance with cow comfort and mastitis control recommendations on mastitis outcomes in smallholder dairy farms in Central Kenya","authors":"E.K. Ng'ang'a ,&nbsp;J. VanLeeuwen ,&nbsp;G.K. Gitau ,&nbsp;S.L. McKenna ,&nbsp;L.C. Heider ,&nbsp;G.K. Keefe","doi":"10.1016/j.rvsc.2024.105469","DOIUrl":"10.1016/j.rvsc.2024.105469","url":null,"abstract":"<div><div>A randomized controlled trial was conducted in Kenya in 2020 with the objectives being to assess compliance of smallholder dairy farmers (SDF) with farm-specific mastitis and cow comfort recommendations, to determine factors associated with compliance, and to determine the impact of these recommendations in reducing cases of subclinical mastitis.</div><div>A total of 114 SDFs (124 cows) were recruited into the study and randomly allocated into intervention (74 farms) and control (40 farms) groups during the first farm visit. Existing farm-level mastitis control and cow comfort strategies were assessed in both intervention and control farms. Cow parameters (e.g. udder hygiene) were observed or measured from individual cows. A California Mastitis Test (CMT) was done on all quarters and scored from 0 (negative) to 3 (strong positive). All quarters with a CMT score of 2 and 3 were treated with intramammary antibacterial cefapirin preparation according to the label (Cefalak®). Intervention farms received farm-specific mastitis and cow comfort recommendations at the end of the first visit. On the second farm visit, cow parameters were measured again, CMT was repeated on all quarters on all cows, and compliance with recommendations was assessed and given a percentage score. Control farms were then given their farm-specific recommendations. Regression models were utilized to assess associations with compliance and improvement in CMT scores between visits.</div><div>Intervention farms received an average of 3.3 and 3.9 recommendations pertaining to mastitis control and cow comfort, respectively. Farms complied with an average of 2.8 and 2.1 mastitis and cow comfort recommendations, respectively, leading to an overall average compliance score of 63.2 % for all recommendations. On the first visit, CMT scores 1 and 2 were significantly higher in quarters among cows on the intervention than control farms. There was a significantly lower proportion of quarters with CMT scores 1 and 2 in the intervention group on the second visit than the first visit and no quarters with CMT score of 3. The principal farmer, type of recommendation and number of recommendations given were significantly associated with compliance to the recommendations. The principal farmer and their age group were significantly associated with quarter CMT scores improving between the first and second visits.</div><div>Farm-specific mastitis and cow comfort recommendations can significantly reduce the number of cases of subclinical mastitis in SDFs and should thus be included in farmers' training. Farmers should be given a small number of recommendations at a time to enhance compliance.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105469"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and herd-level risk factors associated with Brucella infection in smallholders keeping large ruminants","authors":"Abrar Hussain ,&nbsp;Sabir Hussain ,&nbsp;Mamoona Chaudhry ,&nbsp;Muhammad Asif Ali ,&nbsp;Ibrahim Elsohaby ,&nbsp;Ubaid-ur-Rehman Zia ,&nbsp;Olivier Sparagano","doi":"10.1016/j.rvsc.2024.105506","DOIUrl":"10.1016/j.rvsc.2024.105506","url":null,"abstract":"<div><div>Brucellosis is a disease that poses a higher risk of transmission to animals and people who have close interactions with them, such as farmers. Therefore, the present study was designed to determine the seroprevalence of bovine brucellosis and associated risk factors in Kasur, Punjab, Pakistan. One hundred pooled milk samples from 100 smallholder mixed herds of cows and buffaloes with a total study population of 425 animals (277 cattle and 148 buffalos) with a range of up to seven animals in each herd were collected and tested through a milk ring test (MRT). Blood samples were collected from the animals of MRT-positive herds and then subjected to an indirect enzyme-linked immunosorbent assay (i-ELISA) for the presence of antibodies against <em>Brucella abortus.</em> Of the 100 herds, 16 % (<em>n</em> = 16) were MRT-positive. In MRT-positive herds, 77 animals (43 female cattle, three male cattle, 21 female buffaloes, and ten male buffaloes) were present; 32 (41.6 %) were i-ELISA seropositive, with the highest seropositivity (54.8 %) observed in buffaloes as compared to cows (32.6 %). Almost 38 % of the farmers were unaware of brucellosis. Herd-level risk factors indicated <em>Brucella</em> seropositivity increased in herds with recent abortion history and bull exchange during breeding. Conversely, herds with veterinary assistance exhibited a decreased <em>Brucella</em> seropositivity. The findings highlight the need for a government-led awareness campaign emphasizing routine screenings, especially for breeding bulls, to stop further disease spread. Early detection and management can enhance animal health, milk production, and livestock holders' economic well-being.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105506"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the sarcoptic mange upon metabolome profiling in wild boars","authors":"Nadia Piscopo ,&nbsp;Michele Costanzo ,&nbsp;Monica Gelzo ,&nbsp;Luigi Sacchettino ,&nbsp;Chiara Vitiello ,&nbsp;Anna Balestrieri ,&nbsp;Francesco Napolitano ,&nbsp;Luigi Esposito","doi":"10.1016/j.rvsc.2024.105505","DOIUrl":"10.1016/j.rvsc.2024.105505","url":null,"abstract":"<div><div>Sarcoptic mange is a highly contagious disease and represents one of the main health concerns for humans and non-human mammals worldwide. It is caused by the mite <em>Sarcoptes scabiei</em> and can course with different morphological and physiological presentations. Accordingly, aside from skin inflammation, hosts may experience changes in body condition, immune system, biochemistry, reproduction, and thermoregulation, although the understanding of the downstream metabolic burden is still missing. In this context, mange-derived fat store depletion and following imbalance of fatty acid composition might contribute to the severity of the illness. The lack of a tool for early detection of this etiological agent often results in significant financial losses for farmers and harm to animal welfare. Therefore, using targeted LC-MS/MS-based metabolomics approach, we sought to investigate the impact of sarcoptic mange upon metabolome profiling in the blood serum of mangy wild boars. Thirteen wild boars were analyzed in three different clinical conditions, namely when they were sick, during the therapeutic treatment with ivermectin, and when they were deemed recovered from the disease. We identified specific long-chain acylcarnitines highly abundant in the blood serum of the subjects within the infection phase, when compared to the ivermectin-treated and healthy conditions. Overall, data from our preliminary study highlighted the need for more accurate and broad-based studies, about the potential role of the long chain acylcarnitines in the metabolic homeostasis, to help early diagnose of the sarcoptic mange.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105505"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}