Research in veterinary science最新文献

{"title":"Crosstalk between myostatin and callipyge in CRISPR/Cas9-edited goat fibroblast cells","authors":"Helia Fathpour ,&nbsp;Mansooreh Fouladi ,&nbsp;Farnoosh Jafarpour ,&nbsp;Reza Moradi-Hajidavaloo ,&nbsp;Tayebeh Izadi ,&nbsp;Hanieh Shiralian-Esfahani ,&nbsp;Wilfried Kues ,&nbsp;Mohammad-Hossein Nasr-Esfahani ,&nbsp;Mehdi Hajian ,&nbsp;Shahin Eghbalsaied","doi":"10.1016/j.rvsc.2025.105992","DOIUrl":"10.1016/j.rvsc.2025.105992","url":null,"abstract":"<div><div>Myostatin (<em>MSTN</em>) and Callipyge (<em>CLPG</em>) genes are key regulators of muscle growth. While MSTN inhibits muscle development, the <em>CLPG</em> mutation induces muscle hypertrophy through a specific imprinted genetic mechanism. The interaction between these genes remains of interest for improving livestock muscle traits. In this study, CRISPR/Cas9 was employed to edit <em>MSTN</em> and <em>CLPG</em> genes in goat fibroblast cells via electrotransfection. Cells were selected using puromycin antibiotic, and gene-editing efficiency was evaluated through Sanger sequencing. Gene expression changes were analyzed using RT-qPCR analysis. <em>MSTN</em> gene knockout resulted in significant downregulation of <em>MSTN</em> and <em>CLPG</em>, while <em>GTL2</em> expression was upregulated by more than 50-fold. Additionally, myosin heavy chain genes (<em>MYH1</em>, <em>MYH3</em>, <em>MYH4</em>) were strongly upregulated, with <em>MYH3</em> 13-fold and <em>MYH4</em> 30-fold increase in the expression. In <em>CLPG</em>-edited cells, the expression of <em>MSTN</em>, <em>TRIM28</em>, and <em>CLPG</em> was reduced, while <em>GTL2</em> was upregulated by 6-fold. <em>MYH3</em> and <em>MYH4</em> expression increased 4-fold in <em>CLPG</em>-edited cells, though the increase was less pronounced compared to <em>MSTN</em>-edited cells. <em>DLK1</em> expression was undetectable in both non-edited control and gene-edited fibroblast cells. Our findings support the interaction between <em>MSTN</em> and <em>CLPG</em>, contributing to the regulation of muscle growth. Notably, the study also highlights the challenges associated with editing imprinted genes like <em>CLPG</em> and suggests that <em>TRIM28</em> may play a role downstream of <em>CLPG</em> regulation. These results provide valuable insights into muscle development regulation, offering potential applications in livestock genetic improvement.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105992"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing Ebselen for a potential blending-based therapeutic strategy against gastrointestinal nematodes of small ruminants","authors":"Irineu Romero-Neto ,&nbsp;Júlia Dall'Anese ,&nbsp;Taynara Gabriele Ribeiro Piano ,&nbsp;Verônica Wosniaki Ferreira ,&nbsp;Alda Lúcia Gomes Monteiro ,&nbsp;Leandro Piovan ,&nbsp;Marcelo Beltrão Molento","doi":"10.1016/j.rvsc.2025.105980","DOIUrl":"10.1016/j.rvsc.2025.105980","url":null,"abstract":"<div><div>Gastrointestinal nematode (GIN) infections pose a significant challenge to the health of ruminant animals. The infection also presents a substantial financial impact worldwide. This study aimed to test the anthelmintic effect of Ebselen (C₁₃H₉NOSe), a selenium-containing organochalcogen compound, against eggs and third-stage larvae (L3) of GIN of small ruminants. Ebselen and ivermectin (IVM) were evaluated using the egg hatch test (EHT) and the larval migration inhibition test (LMIT). Additionally, we tested the inhibitory effect of drug-drug interactions using both compounds. The compounds individually showed concentration-dependent effect on eggs and L3. Ebselen inhibited 50 % of the eggs and L3 at concentrations of 0.4835 mmol L⁻¹ and 1.562 mmol L⁻¹, respectively. IVM had an half-maximal inhibitory concentration (IC₅₀) value of 0.4449 mmol L⁻¹ on eggs and 0.9141 mmol L⁻¹ on L3. IVM had an efficacy of 96.6 % in avoiding egg hatch and 78.4 % in preventing L3 migration at the highest concentration. The combination of Ebselen and IVM resulted in a statistically significant increase in larval migration inhibition, demonstrating a synergistic effect (&gt;30 %). The data confirm the use of Ebselen and IVM in a novel blending-based therapeutic strategy to manage GIN that affects small ruminants.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105980"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hair samples for PCR detection of Leishmania infantum in dogs","authors":"Geovanna Vioti ,&nbsp;João Augusto Franco Leonel ,&nbsp;Trícia Maria Ferreira de Sousa Oliveira ,&nbsp;Diogo Tiago da Silva ,&nbsp;Maria Luana Alves ,&nbsp;Andrea Cristina Higa Nakaghi ,&nbsp;Lara Borges Keid ,&nbsp;Rodrigo Martins Soares","doi":"10.1016/j.rvsc.2025.105979","DOIUrl":"10.1016/j.rvsc.2025.105979","url":null,"abstract":"<div><div>Canine leishmaniosis (CanL) caused by <em>Leishmania infantum</em> remains a major public health concern in endemic regions, where dogs are the primary domestic reservoir. Despite high accuracy, the invasiveness of current diagnostic methods limits their practical application. This study evaluated the diagnostic potential of hair as a non-invasive sample for molecular detection of L. <em>infantum</em> using a nested PCR targeting the ITS1 region (HAIR-test). All PCR-based tests in this study, including those performed on lymph node aspirates (LN-test), blood (BL-test), and conjunctival swabs (CS-test), employed the same nested ITS1-PCR protocol. A cohort of 44 naturally infected dogs, defined by parasitological confirmation or LN-test positivity, was evaluated using the HAIR-test, with hair samples independently collected by two different operators. The combined sensitivity of the HAIR-test was 59.1 %, outperforming the BL-test and matching the performance of the CS-test. Sequencing confirmed L. <em>infantum</em> DNA in hair samples, but also revealed co-amplification of non-<em>Leishmania</em> trypanosomatids in a subset, underscoring the need for high-specificity assays. Inter-collector agreement was moderate, indicating potential variability in parasite distribution or inconsistencies in sample collection and handling. No correlation was observed between hair mass and PCR-positivity, suggesting that L. <em>infantum</em> DNA is present in follicular bulbs rather than in hair shafts. Beyond suboptimal sensitivity, limitations in assay reproducibility and uncertainties regarding the precise origin of the detected DNA underscore the need for further methodological refinement and validation. The HAIR-test offers a viable, non-invasive alternative in settings where invasive sampling is impractical, but its routine diagnostic application requires methodological refinements and validation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105979"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital sternal cleft in a DSH cat: A case report","authors":"Seyyed Hossein Modarres Tonekabony ,&nbsp;Sarang Soroori ,&nbsp;Parham Soufizadeh ,&nbsp;Kimia Hajarizadeh","doi":"10.1016/j.rvsc.2025.105976","DOIUrl":"10.1016/j.rvsc.2025.105976","url":null,"abstract":"<div><div>The sternum, essential for protecting intrathoracic structures and enabling thoracic stability and breathing motion, is a vital part of small animal thoracic anatomy. Evaluating the sternum is crucial in small animal practice, with typical anatomy including eight to nine sternebrae and species-specific variations. Congenital sternal anomalies, such as pectus excavatum, pectus carinatum, and sternal cleft, though rare, can have significant clinical implications. Sternal cleft, characterized by incomplete fusion of sternebrae, is particularly uncommon in veterinary literature, with limited documented cases. A 2-year-old neutered male domestic shorthair cat, presenting with vomiting and anorexia, underwent comprehensive diagnostic workup at the Small Animal Veterinary Hospital. Laboratory tests indicated leukocytosis and renal dysfunction. Imaging revealed enlarged kidneys, dilation of the urinary system, mild bronchointerstitial lung patterns, and a unique sternal structure. CT and 3D reconstruction confirmed a complete “U”-shaped sternal cleft involving all sternebrae except manubrium process and seventh sternebra. Supportive treatment was administered, but the cat succumbed to systemic complications. Congenital sternal abnormalities in small animals, including sternal cleft, are rare and often associated with other thoracic or abdominal defects. This case highlights the complexity of diagnosing and managing such anomalies, emphasizing the importance of imaging for accurate assessment. This report of a complete “U”-shaped sternal cleft in a cat without respiratory symptoms underscores the necessity of documenting congenital anomalies. Comprehensive diagnostic imaging and multidisciplinary management are crucial for improving outcomes in similar cases. Despite the rarity of sternal cleft, reporting these cases enhances understanding and informs clinical decision-making.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105976"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum iron levels as a biomarker for monitoring fracture healing in dogs: A longitudinal study","authors":"Ersoy Baydar ,&nbsp;Ugur Aydogdu ,&nbsp;Feyyaz Kaya ,&nbsp;Muharrem Erol","doi":"10.1016/j.rvsc.2025.105993","DOIUrl":"10.1016/j.rvsc.2025.105993","url":null,"abstract":"<div><div>Iron is an important mineral involved in various physiological processes and can be used as a biomarker, since its serum concentration changes during the inflammatory response. Eight crossbreed dogs with closed fractures in the antebrachium bone were included in the study. The fracture was treated with a closed reduction method and blood samples were taken initially at 0 h and subsequently at 7, 14, 21, 28, and 45 days. Iron, ferritin, hepcidin, tumor necrosis factor-alpha (TNF-α), C reactive protein (CRP), bone alkaline phosphatase (BALP), osteocalcin, total antioxidant status (TAS), total oxidant status (TOS) as well as routine hemogram and biochemistry analyses were performed in the blood samples taken. It was determined that the decrease in serum Fe levels reversed as the process progressed even though hepcidin, ferritin, osteocalcin, BALP, and TNF-α levels increased at the beginning of the healing process. As a result, it can be suggested that the analysis of serum Fe levels may be a useful biomarker in monitoring the fracture healing process.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105993"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracheal resection and anastomosis combined with the application of collagen-PVP in a dog with traumatic tracheal rupture: A new approach to improve tracheal healing","authors":"Axel E. Guzmán-Cedillo ,&nbsp;Mariana Silva-Martínez ,&nbsp;Roxana Pulido-Quintero ,&nbsp;Patricia Rizo ,&nbsp;J. Raúl Olmos-Zúñiga","doi":"10.1016/j.rvsc.2025.105978","DOIUrl":"10.1016/j.rvsc.2025.105978","url":null,"abstract":"<div><div>The treatment for tracheal injuries is tracheal resection and anastomosis (TRA). Although there are few reports in the veterinary literature, TRA can be complicated and lead to the development of tracheal stenosis, fistulas, or malacias. In human medicine and experimentally in dogs, to avoid such sequelae, TRA combined with wound healing modulators (WHM), such as polyvinyl-pyrrolidone collagen (collagen-PVP), can be applied. In this report, we present a case of tracheal rupture in a dog caused by a bite and treated with TRA combined with collagen-PVP. We observed that from the first week after surgery, the animal presented good clinical evolution without complications, and at 6 months, tracheoscopy revealed normal scarring of the anastomosis; therefore, we believe further studies are worthwhile for its potential clinical application.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105978"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cost divide: Why veterinary medicinal products for dogs and cats in the EU cost more than their human equivalents","authors":"Alessandro Stasi ,&nbsp;Alfonso Pellegrino","doi":"10.1016/j.rvsc.2025.105977","DOIUrl":"10.1016/j.rvsc.2025.105977","url":null,"abstract":"<div><div>This paper provides a comprehensive analysis of the European Union (EU) pricing policies for <strong>veterinary medicinal products (VMPs)</strong>, focusing on the comparative costs of human and pet veterinary formulations of common medications. It examines market dynamics, regulatory frameworks, and structural factors that influence price disparities, drawing on case studies from Italy, France, and Germany. The analysis highlights the role of Regulation (EU) 2019/6, the evolution of the EU VMP market, and the specific economic and regulatory conditions that allow for significant price differences despite identical active ingredients. Using comparative data on furosemide, amoxicillin, benazepril, and prednisone, the paper identifies how lack of public price negotiation, high VAT rates, concentrated market structure, and limitations in pet insurance coverage contribute to affordability challenges. The findings provide policy-relevant insights into how the EU could better align VMP pricing with principles of accessibility, sustainability, and innovation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105977"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoid-based platforms in livestock: Current advances and future prospects","authors":"Dong-Hyeok Kwon ,&nbsp;Hanbyul Kwon ,&nbsp;Goo Jang","doi":"10.1016/j.rvsc.2025.105985","DOIUrl":"10.1016/j.rvsc.2025.105985","url":null,"abstract":"<div><div>Organoid research has been extensively developed and investigated in humans and rodents; however, its applications in livestock species remain comparatively limited. To address this knowledge gap, this review provides a comprehensive synthesis of the historical evolution, establishment protocols, and current organ-specific applications of livestock-derived organoids, spanning tissues such as the intestine, liver, lung, kidney, testis, mammary gland, and reproductive tract. These self-organizing three-dimensional cultures serve as powerful platforms for investigating fundamental questions in veterinary science, from modeling host–pathogen interactions in infectious diseases to exploring the complex biology of reproduction, toxicology, and productivity. Additionally, recent advances in bioengineering and genome-editing technologies, including CRISPR/Cas9, base editing, and prime editing, are highlighted for their potential to enhance the physiological relevance and reproducibility of livestock organoid systems. Despite ongoing challenges, including the lack of standardized protocols, limited availability of pluripotent stem cell–derived models, and insufficient multicellular complexity, livestock organoids represent a strategically important frontier in veterinary science and agricultural biotechnology. In conclusion, the combination of stem cell biology, microphysiological platforms, and genetic engineering positions livestock organoids as indispensable tools for veterinary medicine, agricultural innovation, and translational biomedical research.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105985"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacilli isolated from chickens of Italian native breeds: Antimicrobial susceptibility profile, probiotic potential and anti-Salmonella activity","authors":"Ilaria Resci ,&nbsp;Marta Raffaelli ,&nbsp;Francesca Pedonese ,&nbsp;Mario Forzan ,&nbsp;Filippo Fratini ,&nbsp;Sonia Salvucci ,&nbsp;Beatrice Torracca ,&nbsp;Margherita Marzoni ,&nbsp;Barbara Turchi","doi":"10.1016/j.rvsc.2025.105988","DOIUrl":"10.1016/j.rvsc.2025.105988","url":null,"abstract":"<div><div>Salmonellosis is the second most prevalent foodborne disease in Europe, with eggs, mixed foods, and broiler meat being the primary sources of transmission. Among human salmonellosis cases, the most prevalent <em>Salmonella enterica</em> serovars are <em>S.</em> Enteritidis, <em>S.</em> Typhimurium, and monophasic S. Typhimurium. However, <em>S.</em> Infantis and <em>S.</em> Newport are the most frequently detected serovars in broiler and turkey meat, respectively. Over time, <em>Salmonella</em> spp. have developed multidrug resistance, and the global rise of antimicrobial resistance has driven the search for alternative strategies to antimicrobials use. Probiotics represent a promising alternative to antimicrobials, with the added benefit of enhancing production performance. This study aimed to isolate lactobacilli from chickens of Italian native breeds to evaluate their antimicrobial susceptibility, probiotic potential (tolerance to acidic pH and bile salts), and anti-<em>Salmonella</em> activity against <em>S. enterica</em> strains previously isolated from poultry meat. Antimicrobial susceptibility testing was carried out on all 205 obtained isolates as a preliminary screening. Only nine isolates (4.4 %) were susceptible to all tested antimicrobials. Among them, one strain exhibited notable probiotic potential and anti-<em>Salmonella</em> activity. <em>Lactobacillus kitasatonis</em> VALFI55 demonstrated survival at acidic pH for up to 120 min and tolerance to bile salt concentrations of 0.3 % and 1 %, with limited reduction in viable cell counts compared to other isolates. Furthermore, <em>L. kitasatonis</em> VALFI55 exhibited the strongest inhibitory effect, particularly against <em>S.</em> Typhimurium and <em>S.</em> Infantis. The VALFI55 strain represents a promising probiotic candidate for application in poultry breeding. However, further in vivo studies are required to confirm its beneficial effects.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105988"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared pupillometry to measure pupil light reflex in awake cats: Feasibility and evaluation pre- and post-opioid administration","authors":"Mathieu Magnin ,&nbsp;Léa Goffette ,&nbsp;Stéphane Junot ,&nbsp;Jeanne Marie Bonnet-Garin ,&nbsp;Bernard Allaouchiche ,&nbsp;Céline Pouzot-Nevoret","doi":"10.1016/j.rvsc.2025.105982","DOIUrl":"10.1016/j.rvsc.2025.105982","url":null,"abstract":"<div><div>Assessing pupil diameter and pupillary light reflexes (PLR) in a clinical setting can be challenging due to the pupil's sensitivity to light and the rapid nature of these reflexes. Pupillometry is a technique that is helpful in human medicine in anesthesiology and critical care medicine. However, evidence in veterinary medicine is scarce on the application of pupillometry and analgesic response. Therefore, the aim of this pilot observational study was to assess the feasibility and reliability of portable pupillometry in cats, identify individual factors influencing pupillometric parameters, and compare measurements before and after analgesic administration. Twenty-eight cats, including healthy and hospitalized sick cats, underwent pupillometry using a Neurolight® device. Pupil diameter, PLR parameters, and 4 A-VET pain score were measured before and after opioid administration. Test-retest reliability was evaluated, and the influence of factors was analyzed using multivariate models. Pupillometry was feasible in 97 % of cats, with measurements being easier in hospitalized sick cats. Good reliability was observed for pupil diameter measurements, while PLR parameters showed poor reliability. Weight was significantly associated with pupil size, potentially due to the relationship between body weight and eyeball size. No association was found between pain scores and pupil diameter, and no significant changes in pupil size were observed after opioid administration. In conclusion, portable pupillometry using the Neurolight® is feasible in cats and provides reliable pupil diameter measurements. However, the device is not suitable for objective PLR assessment in this species. Further studies are needed to better understand the factors influencing pupillometry measurements in cats.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105982"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}