Research in veterinary science最新文献

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Effect of cranial tibial artery laceration on radiographic bone healing and perioperative complications in dogs undergoing tibial plateau leveling osteotomy 颅胫动脉裂伤对接受胫骨平台平整截骨术的狗的放射骨愈合和围手术期并发症的影响
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-06-02 DOI: 10.1016/j.rvsc.2024.105322
Francesco Ferrari , Roberto Tamburro , Maurizio Longo , Federica Alessandra Brioschi , Luigi Auletta , Damiano Stefanello
{"title":"Effect of cranial tibial artery laceration on radiographic bone healing and perioperative complications in dogs undergoing tibial plateau leveling osteotomy","authors":"Francesco Ferrari ,&nbsp;Roberto Tamburro ,&nbsp;Maurizio Longo ,&nbsp;Federica Alessandra Brioschi ,&nbsp;Luigi Auletta ,&nbsp;Damiano Stefanello","doi":"10.1016/j.rvsc.2024.105322","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105322","url":null,"abstract":"<div><p>During tibial plateau leveling osteotomy (TPLO), the laceration of the cranial tibial artery (LCTA) may occur, and the ligation of the cranial tibial artery might lead to impaired blood supply to the osteotomy site. The present case-control study aimed to evaluate the effect of LCTA on TPLO healing and the occurrence of perioperative complications. The incidence and predisposing factors to LCTA were also investigated.</p><p>Fourteen cases experiencing LCTA were retrospectively enrolled from medical records of two veterinary teaching hospitals (LCTA group), whereas 28 randomly selected TPLOs that did not experience LCTA were included in the control group. Signalment data, proximal tibial epiphysis conformation, osteotomy features, perioperative complications, and bone healing were compared between the two groups. Bone healing was evaluated using the modified radiographic union scale for tibial fracture and the visual analog scale.</p><p>The mean incidence was 9.6%. Bodyweight was significantly higher in the LCTA group compared to the control group (<em>P</em> = 0.009). Dogs belonging to the LCTA groups were significantly younger (<em>P</em> = 0.01). Intraoperative hypotension was significantly overreported in the LCTA group (<em>P</em> = 0.0001). None of the other variables differed significantly between the two groups.</p><p>Dogs' size seems to be a predisposing factor, with dogs weighing &gt;15 kg having 22 times more chance of experiencing LCTA. Due to the well-developed collateral blood supply of the canine hindlimb, LCTA and the closure of the cranial tibial artery did not appear to delay the radiographic bone healing or affect the incidence of perioperative complications.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105322"},"PeriodicalIF":2.4,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824001887/pdfft?md5=2a6fa47ebb0d8afd8ecf4e43d5f326a6&pid=1-s2.0-S0034528824001887-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141264029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mesenchymal stem cell transplantation: Systematic review, meta-analysis and clinical applications for acute kidney injury and chronic kidney disease in dogs and cats 间充质干细胞移植:系统性综述、荟萃分析及犬猫急性肾损伤和慢性肾病的临床应用
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105313
Leonardo Gaspareto dos Santos, Priscila Inês Ferreira, Alexandre Krause
{"title":"Mesenchymal stem cell transplantation: Systematic review, meta-analysis and clinical applications for acute kidney injury and chronic kidney disease in dogs and cats","authors":"Leonardo Gaspareto dos Santos,&nbsp;Priscila Inês Ferreira,&nbsp;Alexandre Krause","doi":"10.1016/j.rvsc.2024.105313","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105313","url":null,"abstract":"<div><p>Chronic kidney disease (CKD) and acute kidney injury (AKI) are diseases which affect the urinary tract characterized by the loss of renal function. Their therapy requires different therapeutic goals. Mesenchymal stem cells (MSC) transplantation has spread over the years as a treatment for many diseases. In the urinary tract, studies report anti-inflammatory, antiapoptotic, antifibrotic, antioxidant and angiogenic effects. This work reports the results of a meta-analysis about the effects of the MSC application in serum levels of creatinine in dogs and cats with AKI and CKD. The work followed PRISMA guidelines. Data were screened, selected, and extracted with characteristics about the studies. The kinds of injury were classified according to their identification and the risk of bias was calculated by the system SYRCLE. The results of each group were combined by the inverse variance method. The heterogeneity was evaluated by the I<sup>2</sup> test. For the mean of creatinine, a meta-analysis was performed according to the study group and number of applications and separately for the control and treatment groups according to the kind of injury, dose, application route, and moment. At all, 4742 articles were found. Of these, 40 were selected for eligibility, 16 underwent qualitative analysis and 9 to the quantitative. The results denote advantage to the group treated with MSC over placebo. A statistical difference was observed both in combined analysis and in the subgroups division. However, a high heterogeneity was found, which indicates considerable variation between the studies, which indicates caution in generalize the results.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105313"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141292433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Artificial intelligence in veterinary diagnostic imaging: Perspectives and limitations 兽医诊断成像中的人工智能:前景与局限
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105317
Silvia Burti , Tommaso Banzato , Simon Coghlan , Marek Wodzinski , Margherita Bendazzoli , Alessandro Zotti
{"title":"Artificial intelligence in veterinary diagnostic imaging: Perspectives and limitations","authors":"Silvia Burti ,&nbsp;Tommaso Banzato ,&nbsp;Simon Coghlan ,&nbsp;Marek Wodzinski ,&nbsp;Margherita Bendazzoli ,&nbsp;Alessandro Zotti","doi":"10.1016/j.rvsc.2024.105317","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105317","url":null,"abstract":"<div><p>The field of veterinary diagnostic imaging is undergoing significant transformation with the integration of artificial intelligence (AI) tools. This manuscript provides an overview of the current state and future prospects of AI in veterinary diagnostic imaging.</p><p>The manuscript delves into various applications of AI across different imaging modalities, such as radiology, ultrasound, computed tomography, and magnetic resonance imaging. Examples of AI applications in each modality are provided, ranging from orthopaedics to internal medicine, cardiology, and more. Notable studies are discussed, demonstrating AI's potential for improved accuracy in detecting and classifying various abnormalities.</p><p>The ethical considerations of using AI in veterinary diagnostics are also explored, highlighting the need for transparent AI development, accurate training data, awareness of the limitations of AI models, and the importance of maintaining human expertise in the decision-making process. The manuscript underscores the significance of AI as a decision support tool rather than a replacement for human judgement.</p><p>In conclusion, this comprehensive manuscript offers an assessment of the current landscape and future potential of AI in veterinary diagnostic imaging. It provides insights into the benefits and challenges of integrating AI into clinical practice while emphasizing the critical role of ethics and human expertise in ensuring the wellbeing of veterinary patients.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105317"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824001838/pdfft?md5=47a045a989df859b813a3b072b74ebd0&pid=1-s2.0-S0034528824001838-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141264026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beware with the backpack! New hosts and pathogens identified for Ixodes simplex ticks collected from bats in the Iberian Peninsula 小心背包!从伊比利亚半岛的蝙蝠身上发现的单纯蜱新宿主和病原体。
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105316
Alberto Moraga-Fernández , Marta Sánchez-Sánchez , Clara Muñoz-Hernández , Xosé Pardavila , Jorge Sereno-Cadierno , João Queirós , Joaquín Vicente , Isabel G. Fernández de Mera
{"title":"Beware with the backpack! New hosts and pathogens identified for Ixodes simplex ticks collected from bats in the Iberian Peninsula","authors":"Alberto Moraga-Fernández ,&nbsp;Marta Sánchez-Sánchez ,&nbsp;Clara Muñoz-Hernández ,&nbsp;Xosé Pardavila ,&nbsp;Jorge Sereno-Cadierno ,&nbsp;João Queirós ,&nbsp;Joaquín Vicente ,&nbsp;Isabel G. Fernández de Mera","doi":"10.1016/j.rvsc.2024.105316","DOIUrl":"10.1016/j.rvsc.2024.105316","url":null,"abstract":"<div><p>To improve the knowledge on the role of bats in the maintenance and transmission of tick-borne pathogens, a molecular approach was used to characterize <em>Anaplasma</em> spp., <em>Rickettsia</em> spp., <em>Coxiella burnetii</em>, <em>Borrelia burgdorferi</em> s.l., piroplasmids, <em>Hepatozoon</em> spp., flaviviruses and nairoviruses in ticks collected from Iberian bats. A total of 732 bats from 25 species were captured at 38 sampling sites distributed in seven provinces of Spain between 2018 and 2022. Seventy-nine <em>Ixodes simplex</em> ticks were collected from 31 bats (<em>Eptesicus isabellinus</em>, <em>Hypsugo savii</em>, <em>Myotis capaccini</em>, <em>Myotis emarginatus</em>, <em>Myotis myotis</em>, <em>Miniopterus schreibersii</em>, <em>Pipistrellus pipistrellus</em> and <em>Rhinolophus ferrumequinum</em>). Sixty of 79 <em>I. simplex</em> were positive for at least one pathogen tested and were collected from 23 bats captured in southeast Spain. We detected the presence of <em>Rickettsia slovaca</em> in 12 ticks collected from <em>M. emarginatus</em>, <em>H. savii</em>, <em>M. schreibersii</em> and <em>E. isabellinus</em>; <em>Rickettsia aeschlimannii</em> in 1 tick from <em>M. schreibersii</em>; <em>Anaplasma ovis</em> in 3 ticks from <em>H. savii</em> and <em>M. schreibersii</em>; <em>C. burnetii</em> in 2 ticks from <em>H. savii</em>; <em>Occidentia massiliensis</em> in 1 tick from <em>H. savii</em>; piroplasmids in 12 ticks from <em>H. savii</em>, <em>M. schreibersii</em> and <em>E. isabellinus</em>; and a novel nairovirus in 1 tick from <em>M. schreibersii</em>. Furthermore, blood samples obtained from 14 of the 31 tick-infested bats were negative in all PCR analyses. This study describes new host and pathogen associations for the bat-specialist <em>I. simplex</em>, highlights the risk of spread of these pathogens, and encourages further research to understand the role of Iberian bats in the epidemiology of tick-borne pathogens.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"176 ","pages":"Article 105316"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824001826/pdfft?md5=5693a593d269f2ffdfacd6ada652e88f&pid=1-s2.0-S0034528824001826-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systemic AL kappa chain amyloidosis in a captive Bornean orangutan (Pongo pygmaeus) 一只圈养的婆罗洲红毛猩猩(Pongo pygmaeus)患上了全身性 AL kappa 链淀粉样变性病
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105315
Susumu Iwaide , Hitoshi Takemae , Mami Oba , Kenta Owaku , Natsumi Kobayashi , Yoshiyuki Itoh , Takuma Kozono , Miki Hisada , Takako Miyabe-Nishiwaki , Koshiro Watanuki , Tokuma Yanai , Hisafumi Inoue , Tomoaki Murakami
{"title":"Systemic AL kappa chain amyloidosis in a captive Bornean orangutan (Pongo pygmaeus)","authors":"Susumu Iwaide ,&nbsp;Hitoshi Takemae ,&nbsp;Mami Oba ,&nbsp;Kenta Owaku ,&nbsp;Natsumi Kobayashi ,&nbsp;Yoshiyuki Itoh ,&nbsp;Takuma Kozono ,&nbsp;Miki Hisada ,&nbsp;Takako Miyabe-Nishiwaki ,&nbsp;Koshiro Watanuki ,&nbsp;Tokuma Yanai ,&nbsp;Hisafumi Inoue ,&nbsp;Tomoaki Murakami","doi":"10.1016/j.rvsc.2024.105315","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105315","url":null,"abstract":"<div><p>Systemic amyloid light-chain (AL) amyloidosis is an infrequent disease in which amyloid fibrils derived from the immunoglobulin light chain are deposited in systemic organs, resulting in functional impairment. This disease has been notably uncommon in animals, and nonhuman primates have not been reported to develop it. In this study, we identified the systemic AL kappa chain amyloidosis in a captive Bornean orangutan (<em>Pongo pygmaeus</em>) and analyzed its pathogenesis. Amyloid deposits were found severely in the submucosa of the large intestine, lung, mandibular lymph nodes, and mediastinal lymph nodes, with milder lesions in the liver and kidney. Mass spectrometry-based proteomic analysis revealed an abundant constant domain of the immunoglobulin kappa chain in the amyloid deposits. Immunohistochemistry further confirmed that the amyloid deposits were positive for immunoglobulin kappa chains. In this animal, AL amyloidosis resulted in severe involvement of the gastrointestinal submucosa and lymph nodes, which is consistent with the characteristics of AL amyloidosis in humans, suggesting that AL amyloid may have a similar deposition mechanism across species. This report enhances the pathological understanding of systemic AL amyloidosis in animals by providing a detailed characterization of this disease based on proteomic analysis.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105315"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141250119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Frequency of low-dose dexamethasone suppression test (LDDST) response patterns and their correlation with clinicopathologic signs in dogs suspected of having Cushing's syndrome: A retrospective study 低剂量地塞米松抑制试验 (LDDST) 反应模式的频率及其与疑似库欣综合征犬临床病理症状的相关性:回顾性研究
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105318
Nádia Rebelo , Maria Joana Dias , Ryane Englar , Luísa Mateus , Rodolfo Oliveira Leal
{"title":"Frequency of low-dose dexamethasone suppression test (LDDST) response patterns and their correlation with clinicopathologic signs in dogs suspected of having Cushing's syndrome: A retrospective study","authors":"Nádia Rebelo ,&nbsp;Maria Joana Dias ,&nbsp;Ryane Englar ,&nbsp;Luísa Mateus ,&nbsp;Rodolfo Oliveira Leal","doi":"10.1016/j.rvsc.2024.105318","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105318","url":null,"abstract":"<div><p>A retrospective cross-sectional study was conducted to assess the frequency of low-dose dexamethasone suppression test (LDDST) patterns in canine patients that had clinicopathologic signs consistent with Cushing's syndrome (CS). Medical records for patients of interest (<em>N</em> = 128) were reviewed between January 2014 and December 2020 to analyse and classify LDDST results based upon the following patterns: lack of suppression, partial suppression, complete suppression, escape, or inverse. Complete suppression, lack of suppression, partial suppression, escape, and inverse patterns were identified in 39.1%, 31.2%, 14.1%, 10.1% and 5.5% of cases respectively. LDDST results were also evaluated with respect to clinical signs, serum alkaline phosphatase (ALP) activity, urine specific gravity (USG) and adrenal ultrasonographic findings. There was no association between LDDST patterns and clinical signs (<em>p</em> = 0.11), increased ALP (<em>p</em> = 0.32), USG (<em>p</em> = 0.33) or adrenal ultrasonographic findings (<em>p</em> = 0.19). In all dogs that demonstrated complete suppression or an inverse pattern, CS was excluded by the attending clinician. The diagnosis of CS was also excluded without further exploration in 23.1%, 7.5% and 5.6% of dogs that demonstrated an escape pattern, lack of suppression and partial suppression pattern, respectively. These results suggest that the clinical significance of LDDST patterns, particularly escape and inverse patterns, are misunderstood by some clinicians, leading them to prematurely exclude the diagnosis of CS.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105318"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S003452882400184X/pdfft?md5=00c790e602db3cb43650c34940c844fe&pid=1-s2.0-S003452882400184X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141292380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New insight on porcine carboxylesterases expression and activity in lung tissues 猪肺组织中羧酸酯酶表达和活性的新发现
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105314
Andrea Cavallero , Giorgia Donadel , Paola Puccini , Pier Giovanni Gervasi , Khatia Gabisonia , Vincenzo Longo , Morena Gabriele
{"title":"New insight on porcine carboxylesterases expression and activity in lung tissues","authors":"Andrea Cavallero ,&nbsp;Giorgia Donadel ,&nbsp;Paola Puccini ,&nbsp;Pier Giovanni Gervasi ,&nbsp;Khatia Gabisonia ,&nbsp;Vincenzo Longo ,&nbsp;Morena Gabriele","doi":"10.1016/j.rvsc.2024.105314","DOIUrl":"10.1016/j.rvsc.2024.105314","url":null,"abstract":"<div><p>Over the course of the last twenty years, there has been a growing recognition of the pig's potential as a valuable model for studying human drug metabolism. This study aimed to investigate the expression, enzymatic activity, inhibitory susceptibility, and cellular localization of carboxylesterases (CES) in porcine lung tissue not yet explored. Our results showed that CESs hydrolysis activity followed Michaelis-Menten kinetics in both cytosolic and microsomal fractions of porcine lung tissues (<em>N</em> = 8), with comparable hydrolysis rates for tested substrates, namely 4-nitrophenyl acetate (pNPA), 4-methylumbelliferyl acetate (4-MUA), and fluorescein diacetate (FD). We also determined the CESs hydrolysis activity in a representative sample of the porcine liver that, as expected, displayed higher activity than the lung ones. The study demonstrated variable levels of enzyme activities and interindividual variability in both porcine lung fractions. Inhibition studies used to assess the CESs' involvement in the hydrolysis of pNPA, 4-MUA, and FD suggested that CESs may be the enzymes primarily involved in the metabolism of ester compounds in the pig lung tissue. Overall, this study provides insight into the distribution and diversity of CES isoforms involved in substrate hydrolysis across different cellular fractions (cytosol and microsomes) in porcine lungs.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105314"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824001802/pdfft?md5=d3a1bef7cd6a1090febe8c744b3e5b8f&pid=1-s2.0-S0034528824001802-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Action of mycocins produced by Wickerhamomyces anomalus on Malassezia pachydermatis isolated from the ear canal of dogs Wickerhamomyces anomalus 产生的霉菌素对从狗耳道分离出的马拉色菌的作用。
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105319
Jessica Cassia da Silva , Maria Gabriele Cerny , Bruna Larissa Nascimento , Eloiza Cristina Martelli , Jessica Vieira , Rafaela de Souza Marquezoni , Mario Tatsuo Makita , Claudete Rodrigues Paula , Francisco de Assis Baroni , Rinaldo Ferreira Gandra
{"title":"Action of mycocins produced by Wickerhamomyces anomalus on Malassezia pachydermatis isolated from the ear canal of dogs","authors":"Jessica Cassia da Silva ,&nbsp;Maria Gabriele Cerny ,&nbsp;Bruna Larissa Nascimento ,&nbsp;Eloiza Cristina Martelli ,&nbsp;Jessica Vieira ,&nbsp;Rafaela de Souza Marquezoni ,&nbsp;Mario Tatsuo Makita ,&nbsp;Claudete Rodrigues Paula ,&nbsp;Francisco de Assis Baroni ,&nbsp;Rinaldo Ferreira Gandra","doi":"10.1016/j.rvsc.2024.105319","DOIUrl":"10.1016/j.rvsc.2024.105319","url":null,"abstract":"<div><p>This study aims to examine the effectiveness of mycocins produced by <em>Wickerhamomyces anomalus</em> in inhibiting <em>Malassezia pachydermatis</em>, a yeast commonly found in the ear canal of dogs. <em>M. pachydermatis</em> has a zoophilic origin and can be found in mammals, and frequently in dogs, where it mainly colonizes the ear canal region and the skin, leading to lesions that are difficult to treat. The antimicrobial mechanism was evaluated using dilutions of supernatant with enzymatic activity, which may include β-glucanases, glycoproteins known to act on microorganism cell walls. However, it is important to note that this supernatant may contain other compounds as well. β-glucanases in the mycocins supernatant were found at a concentration of 0.8 U/mg. The susceptibility of <em>M. pachydermatis</em> isolates was tested using the microdilution method. The isolates suffered 100% inhibition when tested with the culture supernatant containing mycocins. In the proteinases production test, 44% of the isolates tested were strong proteinases producers. Subsequently all these isolates suffered inhibition of their activity when tested in research medium containing mycocins supernatant at a subinhibitory concentration of β-glucanases. This shows that mycocins can inhibit the production of proteinases, a virulence factor of <em>M. pachydermatis</em>. The viability test showed the antifungal action of mycocins in inhibiting the viability of <em>M. pachydermatis</em> cells after a period of 8  hours of contact. These results support the antimicrobial potential of mycocins and their promise as a therapeutic option.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"176 ","pages":"Article 105319"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the Surgical Pleth Index (SPI) for the monitoring of the nociception-antinociception balance in dogs undergoing castration: A prospective clinical trial 评估用于监测接受阉割的狗的痛觉-反痛觉平衡的手术痛觉指数 (SPI):前瞻性临床试验
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105320
Morgane Gavet , Martina Cardinali , Angelique Bernady , Clara Conde Ruiz , Bernard Allaouchiche , Stephane Junot
{"title":"Evaluation of the Surgical Pleth Index (SPI) for the monitoring of the nociception-antinociception balance in dogs undergoing castration: A prospective clinical trial","authors":"Morgane Gavet ,&nbsp;Martina Cardinali ,&nbsp;Angelique Bernady ,&nbsp;Clara Conde Ruiz ,&nbsp;Bernard Allaouchiche ,&nbsp;Stephane Junot","doi":"10.1016/j.rvsc.2024.105320","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105320","url":null,"abstract":"<div><p>The aim of this prospective clinical study was to evaluate the efficacy of the Surgical Pleth Index (SPI), a validated nociception monitor in human anaesthesia, in dogs. The technology uses a plethysmographic signal from a specific pulse oximetry probe to analyse pulse wave amplitudes and heartbeat intervals. Twenty-six healthy dogs anaesthetised for castration were included. SPI, invasive mean arterial pressure (MAP) and heart rate (HR) were continuously monitored. The occurrence or resolution of a haemodynamic reaction (HDR), defined as a &gt; 20% increase in HR and/or MAP, was assessed at predefined times: cutaneous incision, testicles' exteriorization, cutaneous suture, and fentanyl administration. Following nociceptive events, the dogs presenting a HDR showed a significant 8% and 10% increase in SPI at 3 and 5 min respectively, whereas after fentanyl administration, a 13% and 16% significant decrease in SPI were noted. Receiver operating characteristic curves analysis indicated a moderate performance for the dynamic variations of SPI over 1 min to predict a HDR (AUC: 0.68, threshold value: +15%) or its resolution after fentanyl administration (AUC of 0.72, threshold value: −15%) within 3 min. The SPI varied according to perioperative nociceptive events and analgesic treatment; however, its performance to anticipate a HDR was limited with high specificity but low sensivity. Refinement of the algorithm to specifically accommodate for the canine species may be warranted. Further studies are required to evaluate the influence of other factors on the performance of this index.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105320"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141250606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The adipokines progranulin and omentin can directly regulate feline ovarian granulosa cell functions 脂肪素原和网膜素能直接调节猫卵巢颗粒细胞的功能
IF 2.4 3区 农林科学
Research in veterinary science Pub Date : 2024-05-31 DOI: 10.1016/j.rvsc.2024.105321
Alexander V. Sirotkin , Zuzana Fabová , Barbora Loncová , Maria Bauerová , Abdel Halim Harrath
{"title":"The adipokines progranulin and omentin can directly regulate feline ovarian granulosa cell functions","authors":"Alexander V. Sirotkin ,&nbsp;Zuzana Fabová ,&nbsp;Barbora Loncová ,&nbsp;Maria Bauerová ,&nbsp;Abdel Halim Harrath","doi":"10.1016/j.rvsc.2024.105321","DOIUrl":"https://doi.org/10.1016/j.rvsc.2024.105321","url":null,"abstract":"<div><p>The aim of the present study was to determine the effects of the adipokines progranulin and omentin on the basic functions of feline ovarian cells. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the proliferation (accumulation of PCNA and cyclin B1), apoptosis (accumulation of Bax and caspase 3) and progesterone release of cultured feline ovarian granulosa cells by quantitative immunocytochemistry and enzyme-linked immunosorbent assays (ELISAs). Both progranulin and omentin increased cell proliferation and decreased apoptosis. Both progranulin and omentin promoted progesterone release. The present findings demonstrate that the adipokines progranulin and omentin can directly regulate basic feline ovarian cell functions.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"175 ","pages":"Article 105321"},"PeriodicalIF":2.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141250120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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