Research in veterinary science最新文献_第10页

Beyond rodents: The integral role of domestic animals in biomedical research 超越啮齿动物：家畜在生物医学研究中不可或缺的角色

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-09 DOI: 10.1016/j.rvsc.2025.105803

Ravindran Kumar Pramod , Vandana Yadav , M.R. Vishnuraj , Mukesh Kumar Gupta

{"title":"Beyond rodents: The integral role of domestic animals in biomedical research","authors":"Ravindran Kumar Pramod , Vandana Yadav , M.R. Vishnuraj , Mukesh Kumar Gupta","doi":"10.1016/j.rvsc.2025.105803","DOIUrl":"10.1016/j.rvsc.2025.105803","url":null,"abstract":"<div><div>Annually, around 100 million vertebrate animals are utilized globally in biomedical research, with rodents, specifically mice and rats, being the most commonly used animals in laboratory settings. While rodent models have substantially advanced our understanding of basic biological mechanisms, the translation of these findings often benefits from the use of large animal models, which offer several key advantages for bridging preclinical research to human applications. Thus, domestic animals are increasingly recognized as valuable laboratory animal models to mirror human diseases, assess pharmaceuticals, vaccines, and biologics; conduct xenotransplantation to alleviate human organ shortages; evaluate medical devices; test new surgical techniques; and assist in medical imaging research (X-ray, computed tomography and magnetic resonance imaging). Sheep, goats, pigs, cattle, dogs, cats, horses, donkeys and rabbits represent key domestic species employed in biomedical research. Their comparable body size and lifespan, anatomical and physiological similarities to human organs, and the resemblance of some of their naturally occurring diseases to human conditions have driven the increased use of domestic animals as laboratory models. Advances in genomic technologies and gene editing have further enhanced the value of domestic animals, enabling the development of tailored disease models and translational studies. Future strategic and sustained investment in genetic engineering, biobanking, and the development of species-specific research tools is essential to fully realize the translational potential of domestic animal models. By examining key examples and emerging trends, this review highlights the indispensable role of different species of domestic animals in bridging the gap between basic research and clinical applications.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105803"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prepartum metabolic profiles as predictors of postpartum health in dairy cows 产前代谢谱作为奶牛产后健康的预测因子

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-08 DOI: 10.1016/j.rvsc.2025.105801

B.G.C. de Bruijn , S.G.A. van der Drift , R.M. de Mol , J.T.N. van der Werf , C. van Weeghel , E.A.M. Bokkers , C.G. van Reenen , I.D.E. van Dixhoorn

{"title":"Prepartum metabolic profiles as predictors of postpartum health in dairy cows","authors":"B.G.C. de Bruijn , S.G.A. van der Drift , R.M. de Mol , J.T.N. van der Werf , C. van Weeghel , E.A.M. Bokkers , C.G. van Reenen , I.D.E. van Dixhoorn","doi":"10.1016/j.rvsc.2025.105801","DOIUrl":"10.1016/j.rvsc.2025.105801","url":null,"abstract":"<div><div>Dairy cows are at risk for postpartum diseases in the transition period and early identification of dry cows at risk can support interventions to improve transition management. The objective of this study was to evaluate the predictive value of several prepartum clinical-chemical serum indicators for identification of dairy cows at increased risk for impaired postpartum health. On four farms, 180 cows were scored on 19 clinical signs twice weekly from two wk prepartum until six wk postpartum. Serum samples were collected between day 25 and 1 prepartum and wk one and five postpartum, and analyzed using colorimetric methods, enzymatic methods or AlphaLISA. Based on postpartum clinical scores and serum variables, a Total Deficit Score (TDS) was calculated and used as integral indicator of postpartum health. Cows were divided into three categories: low TDS (healthy), high TDS (impaired health), or culled within six wk after parturition. Increased prepartum serum concentrations of haptoglobin and total protein, and decreased magnesium and albumin were associated with impaired postpartum health, and were especially deviating for cows culled after parturition. A generalized mixed linear model was developed to detect impaired postpartum health in individual cows and contained parity group, prepartum magnesium and albumin concentrations. Performance of the individual-level prediction model was limited, likely because few cows had serum variables outside the cut-off values in this study. In conclusion, prepartum serum haptoglobin, albumin, total protein, and magnesium concentrations were associated with postpartum health of cows. Farmers could monitor these prepartum serum variables to improve transition cow health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105801"},"PeriodicalIF":2.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic signatures in in vitro-expanded equine chondrocytes induced by a histone deacetylase inhibitor 组蛋白去乙酰化酶抑制剂诱导体外扩增马软骨细胞的表观遗传特征

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-08 DOI: 10.1016/j.rvsc.2025.105800

Tomasz Ząbek , Wojciech Witarski , Tomasz Szmatoła , Ewelina Semik-Gurgul , Sebastian Sawicki , Katarzyna Ropka-Molik

{"title":"Epigenetic signatures in in vitro-expanded equine chondrocytes induced by a histone deacetylase inhibitor","authors":"Tomasz Ząbek , Wojciech Witarski , Tomasz Szmatoła , Ewelina Semik-Gurgul , Sebastian Sawicki , Katarzyna Ropka-Molik","doi":"10.1016/j.rvsc.2025.105800","DOIUrl":"10.1016/j.rvsc.2025.105800","url":null,"abstract":"<div><div>We have explored the impact of DNA methylation changes on gene transcription in expanded equine chondrocytes treated with the histone deacetylase inhibitor (HDACi), Trichostatin A (TSA). The subjects were DNA and RNA samples prepared from articular cartilage cells derived from four animals in our previous study. Using Reduced Representation Bisulfite Sequencing (RRBS), we determined differentially methylated sites (DMS) and regions (DMRs) in the genomes of TSA-treated cells. We linked them to gene differential expression, as obtained from 3’ mRNA sequencing data and the single-locus quantification results of mRNA abundance (Real-time PCR) for the four chondrocyte cell lines. We have identified a set of genes exhibiting a negative correlation between methylation and gene expression, which are involved in cartilage development, cell proliferation, and chromatin organization. While TSA was found to hypermethylate and downregulate genes crucial for chondrocyte function, it also hypomethylated and upregulated genes involved in cartilage and bone formation. The findings highlight the TSA's selective activity in modifying epigenetic marks, suggesting its potential as well as limitations in promoting chondrocyte differentiation and regeneration, which is notably relevant for regenerative medicine applications in treating cartilage damage.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105800"},"PeriodicalIF":2.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence, identification and antimicrobial sensitivity profile of methicillin resistant Staphylococcus colonizing dogs, cats and humans in a veterinary hospital, Brazil 巴西一家兽医医院犬、猫和人对甲氧西林耐药葡萄球菌的流行、鉴定和抗菌敏感性分析

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-05 DOI: 10.1016/j.rvsc.2025.105793

Ricardo Antonio Pilegi Sfaciotte , Leandro Parussolo , Fernanda Danielle Melo , Luis Henrique Pompelli , Naiara Dognani Israel , David Germano Gonçalves Schwarz , Ubirajara Maciel da Costa , Sandra Maria Ferraz

{"title":"Prevalence, identification and antimicrobial sensitivity profile of methicillin resistant Staphylococcus colonizing dogs, cats and humans in a veterinary hospital, Brazil","authors":"Ricardo Antonio Pilegi Sfaciotte , Leandro Parussolo , Fernanda Danielle Melo , Luis Henrique Pompelli , Naiara Dognani Israel , David Germano Gonçalves Schwarz , Ubirajara Maciel da Costa , Sandra Maria Ferraz","doi":"10.1016/j.rvsc.2025.105793","DOIUrl":"10.1016/j.rvsc.2025.105793","url":null,"abstract":"<div><div>The presence of Methicillin-resistant <em>Staphylococcus</em> (MRS) is not limited to humans and sick animals but can also colonize healthy animals and professionals working in veterinary hospitals. The aim of this study was to investigate the presence of MRS in hospitalized animals and staff at a university veterinary hospital, as well as to identify the main species of <em>Staphylococcus</em> and their antimicrobial susceptibility profile. Nasal swabs were collected from 106 animals upon admission to the Veterinary Clinical Hospital and another swab after the patients were discharged from hospital. 20 nasal swabs were collected from staff involved in the routine of the Hospital. Strains identified as MRS were tested for their antimicrobial susceptibility profile and identification of <em>Staphylococcus</em> species by the multiplex PCR. It was found that 48.11 % of the animals were positive for MRS. Of these, 38.7 % were dogs and 10.4 % were cats. From the 76 MRS isolated from animals, 52.63 % were identified as MRSA, 40.76 % as MRSP, and 6.58 % as MRSS. All phenotypically identified MRS carried the <em>mec</em>A gene. As for the individuals, 90 % were colonized by MRS, with 66,67 % by MRSA and 33.33 % by MRSP. The presence of MRS in the staff and, animals highlights the widespread dissemination of these pathogens within a veterinary hospital, contributes to the spread of multidrug-resistant microorganisms.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105793"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance of Escherichia coli and associated factors in pet dogs from Cajamarca city (Peru) 秘鲁卡哈马卡市宠物犬大肠杆菌耐药性及相关因素分析

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-05 DOI: 10.1016/j.rvsc.2025.105799

Rodolfo G. Gamarra-Ramírez, Luis Vargas-Rocha

{"title":"Antimicrobial resistance of Escherichia coli and associated factors in pet dogs from Cajamarca city (Peru)","authors":"Rodolfo G. Gamarra-Ramírez, Luis Vargas-Rocha","doi":"10.1016/j.rvsc.2025.105799","DOIUrl":"10.1016/j.rvsc.2025.105799","url":null,"abstract":"<div><div>The inherent susceptibility of dogs to multiple bacterial infections, combined with inadequate management of antibacterial agents, represents significant public and veterinary health concerns. This study aimed to determine the prevalence of resistance in <em>Escherichia coli</em> isolates to commonly used antibacterials and identify associated resistance factors in pet dogs from Cajamarca city. One hundred fecal samples were collected, from which <em>E. coli</em> strains were cultured and isolated. Antibiogram testing was conducted using the Kirby-Bauer method. Simultaneously, a survey was administered to the owners of the sampled animals to identify risk factors. One hundred percent of the isolates were resistant to at least one antibacterial agent. The prevalence of resistance to streptomycin [STR] (61 %) and ampicillin [AMP] (53 %) was categorized as “very high”. In comparison, resistance to cephalexin [CEP] (47 %), sulfamethoxazole/trimethoprim [SXT] (41 %), gentamicin [GEN] (40 %), and tetracycline [TET] (33 %) was categorized as “high”, and resistance to enrofloxacin [ENR] (10 %) was classified as “low”. Resistance to STR was associated with age, while resistance to SXT was associated with sex and cohabitation with other animal species (<em>p</em> < 0.05). The risk of acquiring STR resistance in <em>E. coli</em> was higher in adult dogs than in younger ones (OR 2.304 [95 % CI: 1.012–5.244], <em>p</em> = 0.047). Regarding SXT, the likelihood of <em>E. coli</em> acquiring resistance in females was higher than in males (OR 3.987 [95 % CI: 1.672–9.088], <em>p</em> = 0.002), and cohabitation with other animal species was also a risk factor (OR 3.468 [95 % CI: 1.258–9.562], <em>p</em> = 0.016).</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105799"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chondroprotective effects of ozone and hyaluronic acid in rat knee osteoarthritis: Comparison of intra-articular and systemic administration 臭氧和透明质酸对大鼠膝关节骨性关节炎的软骨保护作用：关节内和全身给药的比较

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-05 DOI: 10.1016/j.rvsc.2025.105798

Elif Unlu , Nihal Y. Gül Satar , Onur Onguncan , Sabire Güler , Nevzat Uslu , M. Omer Ukum

{"title":"Chondroprotective effects of ozone and hyaluronic acid in rat knee osteoarthritis: Comparison of intra-articular and systemic administration","authors":"Elif Unlu , Nihal Y. Gül Satar , Onur Onguncan , Sabire Güler , Nevzat Uslu , M. Omer Ukum","doi":"10.1016/j.rvsc.2025.105798","DOIUrl":"10.1016/j.rvsc.2025.105798","url":null,"abstract":"<div><div>This study aimed to evaluate the therapeutic efficacy of intra-articular (i.a.) ozone, hyaluronic acid (HA), their combination (ozone–HA), and systemic (intraperitoneal, i.p.) ozone in a rat model of surgically induced knee osteoarthritis (OA). OA was induced by anterior cruciate ligament transection in 60 female Sprague Dawley rats randomized into six groups: i.a. control (saline), i.a. HA, i.a. ozone, i.a. ozone–HA, i.p. control, and i.p. ozone. Treatments were administered weekly for five weeks, starting on day 14 post-surgery. Therapeutic outcomes were assessed using computed tomography, histopathological scoring (Modified Mankin, cartilage degeneration, and matrix loss width), immunohistochemistry (type II collagen, MMP-13), and serum TNF-α levels. At week eight, CT imaging revealed reduced structural damage in the i.a. ozone–HA group. The i.a. ozone–HA group had the lowest Modified Mankin score, significantly lower than the i.a. control (<em>p</em> < 0.001) and i.p. control (<em>p</em> = 0.007) groups. Cartilage matrix loss was significantly reduced in treated groups, particularly in the superficial zone (<em>p</em> = 0.010). Cartilage degeneration scores in the Z1 region were significantly improved in the i.a. ozone (<em>p</em> = 0.011) and i.a. ozone–HA (<em>p</em> = 0.004) groups compared to i.a. control. Additionally, type II collagen expression was significantly enhanced in both i.a. ozone and ozone–HA groups (<em>p</em> = 0.010). These results indicate that intra-articular ozone, particularly combined with HA, offers superior chondroprotective and regenerative effects compared to HA alone or systemic ozone, highlighting its potential as a novel therapeutic approach for early-stage osteoarthritis in veterinary medicine.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105798"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the reliability of the use of infrared thermography in the categorization of tumors in dogs 红外热成像在犬肿瘤分类中的可靠性评价

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-05 DOI: 10.1016/j.rvsc.2025.105785

Keylla Hörbe Steffen dos Santos , Larissa Bennett de Avellar , Daniel Guimarães Gerardi

{"title":"Evaluation of the reliability of the use of infrared thermography in the categorization of tumors in dogs","authors":"Keylla Hörbe Steffen dos Santos , Larissa Bennett de Avellar , Daniel Guimarães Gerardi","doi":"10.1016/j.rvsc.2025.105785","DOIUrl":"10.1016/j.rvsc.2025.105785","url":null,"abstract":"<div><div>Infrared thermography (IRT) has been explored as a complementary diagnostic tool in dogs, particularly in oncology. However, few studies have assessed its applicability to non-neoplastic skin tumors or compared thermal patterns between malignant and benign tumors. This study evaluated IRT as a method for distinguishing thermal patterns between healthy skin and cutaneous tumoral lesions in dogs. A total of 103 skin lesions from 57 dogs, either male or female, of different breeds, and average age of 10 years, were analyzed and classified into benign neoplasms, malignant neoplasms, and non-neoplastic tumors. Mean surface temperatures were obtained for three areas: tumoral (El1), transitional (LiM), and healthy adjacent skin (El2). Rectal temperature was also assessed using a digital thermometer. Results showed that, in the malignant neoplasm group, the temperature of El1 was significantly lower when compared to both El2 and LiM within the same group (<em>p</em>= 0.008). Additionally, El1 exhibited slightly higher average temperatures (0.5‐0.6 °C) in the malignant neoplasm (33.2 ± 2.4 °C) and non-neoplastic group (33.5 ± 1.5 °C) compared to benign neoplasms (32.6 ± 2.5 °C), although this difference was not statistically significant (<em>p</em>=0.301). In conclusion, IRT is a promising tool for differentiating thermal emissions between skin tumors/lesions and healthy skin in dogs. Healthy areas were significantly warmer than affected tissue. Malignant neoplasms and non-neoplastic lesions showed higher thermal means in the tumoral area compared to benign neoplasms.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105785"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary bladder lesions of bovine enzootic hematuria in Argentina 阿根廷牛地方性血尿的膀胱病变

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-04 DOI: 10.1016/j.rvsc.2025.105797

Laura S. Aguirre , Claudio G. Barbeito , Diego M. Medina , Luis A. Colque Caro , Juan F. Micheloud

{"title":"Urinary bladder lesions of bovine enzootic hematuria in Argentina","authors":"Laura S. Aguirre , Claudio G. Barbeito , Diego M. Medina , Luis A. Colque Caro , Juan F. Micheloud","doi":"10.1016/j.rvsc.2025.105797","DOIUrl":"10.1016/j.rvsc.2025.105797","url":null,"abstract":"<div><div>Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105797"},"PeriodicalIF":2.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of pre-slaughter logistics duration on stress responses and coping profiles in commercial finishing pigs 屠宰前物流持续时间对商品育肥猪应激反应和应对特征的影响

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-03 DOI: 10.1016/j.rvsc.2025.105796

Nancy F. Huanca-Marca , Laura X. Estévez-Moreno , Adriana Pastrana-Camacho , Matilde Piñeiro , Gustavo A. María , Genaro C. Miranda-de la Lama

{"title":"Effects of pre-slaughter logistics duration on stress responses and coping profiles in commercial finishing pigs","authors":"Nancy F. Huanca-Marca , Laura X. Estévez-Moreno , Adriana Pastrana-Camacho , Matilde Piñeiro , Gustavo A. María , Genaro C. Miranda-de la Lama","doi":"10.1016/j.rvsc.2025.105796","DOIUrl":"10.1016/j.rvsc.2025.105796","url":null,"abstract":"<div><div>The present study examined the impact of pre-slaughter logistics duration and coping profiles on physiological indicators of stress and inflammation in commercial finishing pigs. A total of 13 journeys involving 2465 commercial pigs (Hybrid DanBred × Piétrain) were monitored. A subset of 359 pigs was selected for blood sampling at slaughter to assess cortisol, glucose, lactate, creatine kinase (CK), Pig-MAP, and C-reactive protein (CRP). The logistics duration were categorised as follows: short logistics time or ST (< 210 min or <3.5 h), medium logistics time MT (>210–300 min or 3.5–5 h), and long logistics time or LT (<em>></em>300 <em>- <</em> 420 min or >5 h). The results obtained demonstrate that acute physiological stress responses are associated withST logistics, as evidenced by elevated cortisol and lactate levels. This phenomenon is presumably attributable to inadequate recovery during the period of lairage subsequent to handling and transportation. It has been demonstrated that MT are associated with elevated glucose levels, which may be indicative of a metabolic adaptation to moderate stress. Conversely, LT were associated with elevated CK levels, suggesting the possibility of muscle fatigue or exertion, potentially attributable to protracted logistics processes. Furthermore, the identification of three coping profiles provides additional insight into individual differences in stress responses and offers a potential framework for the early detection of compromised welfare in commercial settings. These findings underscore the need for tailored transport and lairage strategies that integrate both logistical and animal-based factors, particularly resilience.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105796"},"PeriodicalIF":2.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating somatic cell count, the California mastitis test, and infrared thermography for subclinical mastitis detection in meat ewes 评估体细胞计数、加州乳腺炎试验和红外热成像对肉羊亚临床乳腺炎检测的影响

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-07-02 DOI: 10.1016/j.rvsc.2025.105791

Gretchen L. Peckler, Sarah J.J. Adcock

{"title":"Evaluating somatic cell count, the California mastitis test, and infrared thermography for subclinical mastitis detection in meat ewes","authors":"Gretchen L. Peckler, Sarah J.J. Adcock","doi":"10.1016/j.rvsc.2025.105791","DOIUrl":"10.1016/j.rvsc.2025.105791","url":null,"abstract":"<div><div>Mastitis is a major concern in sheep farming. Detecting subclinical mastitis, which manifests without apparent signs, is particularly challenging. While somatic cell count (SCC) and the California Mastitis Test (CMT) are widely used in dairy animals, and infrared thermography has shown some promise, their diagnostic performance in meat sheep remains under-evaluated. This study aimed to 1) characterize SCC and udder skin surface temperature across lactation in subclinically infected and uninfected udder halves; 2) determine diagnostic cutoffs for SCC, CMT, and thermography; and 3) compare diagnostic performance using receiver operating characteristic curves. Milk and thermal data were collected weekly from 37 clinically healthy nursing ewes over the first 8 weeks postpartum. Infection was defined by ≥100 CFU/mL of a bacterial species. Infected udder halves had higher log SCCs than uninfected halves in weeks 1–4 and 6 postpartum. Infection status did not predict udder skin temperature, which was more influenced by ambient temperature. SCC had the highest diagnostic performance (AUC = 0.78), with an optimal cutoff of 148,500 cells/mL (sensitivity = 0.77; specificity = 0.66). The CMT yielded an AUC of 0.70 with an optimal cutoff score above negative (i.e., trace or positive reaction; sensitivity = 0.77; specificity = 0.51). Udder skin temperature had the lowest AUC (0.56). SCC is the most effective of the three tools for detecting subclinical mastitis, although CMT may serve as a useful on-farm screening tool. Infrared thermography did not detect subclinical infection, highlighting the need for further research into non-invasive diagnostics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105791"},"PeriodicalIF":2.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0