Research in veterinary science最新文献

{"title":"Declining female representation with advancing academic rank in veterinary institutions from non-Western countries","authors":"Ursula Norton ,&nbsp;Rebecca Dunlop ,&nbsp;Rachel Allavena ,&nbsp;María José Navarrete Talloni ,&nbsp;Chiara Palmieri","doi":"10.1016/j.rvsc.2025.106016","DOIUrl":"10.1016/j.rvsc.2025.106016","url":null,"abstract":"<div><div>Gender equity in academic veterinary science remains underexplored outside of Western countries. This study aimed to evaluate gender representation across academic ranks within veterinary institutions in Asia, Sub-Saharan Africa, Latin America, and the Middle East and North Africa.</div><div>Data on academic staff were collected from 96 veterinary schools across 39 countries between September 2023 and April 2024. Gender was inferred through institutional websites, online academic platforms, and machine-learning tools, and academic ranks were standardised into four categories: Lecturer, Assistant Professor, Associate Professor, and Professor. Generalised linear models were used to assess the relationship between gender and academic rank in each region.</div><div>Across all regions, male academics were more represented at higher academic ranks. Female representation declined significantly with increasing rank in all regions (<em>p</em> &lt; 0.0001). The percentage of female academics ranged widely, from 7 % (Nepal) to 68 % (Myanmar and Peru). Asia had the lowest overall female representation (29 %), followed by the Middle East (40 %), Latin America (43 %), and Sub-Saharan Africa (44 %). The decline in female representation across ranks was steepest in Asia and the Middle East, while Sub-Saharan Africa exhibited a slower decline and was the only region where female representation increased at the Professor level compared to Associate Professor.</div><div>Despite regional variation, veterinary academia in non-Western countries remains male-dominated, particularly at senior levels. However, the observed patterns in Africa and Latin America may reflect emerging progress in gender equity. Addressing structural and cultural barriers to academic advancement is essential to support the career progression of women in veterinary science globally.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106016"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ochratoxin A induces intestinal barrier impairment and concurrent hepatorenal toxicity in broiler chicks via modulation of autophagy, ferroptosis, and the intestinal microbiota","authors":"Qixi Li,&nbsp;Ting Wu,&nbsp;Yueqing Xie,&nbsp;Mingzhen Jiang,&nbsp;Xuhai Li,&nbsp;Hongsen Yu,&nbsp;Ershun Zhou,&nbsp;Zhengtao Yang,&nbsp;Jingjing Wang","doi":"10.1016/j.rvsc.2025.106007","DOIUrl":"10.1016/j.rvsc.2025.106007","url":null,"abstract":"<div><div>Ochratoxin A (OTA), a mycotoxin prevalent in contaminated feed, induces systemic toxicity upon intestinal absorption. In this study, broiler chicks were randomly divided into control, vehicle, or OTA-treated groups (0.05, 0.1, and 0.2 mg/kg bw/day). The results indicated that OTA caused histopathological damage to the intestine, liver, and kidney. Notably, OTA at 0.2 mg/kg bw induced hepatocellular injury, as evidenced by significantly elevated serum AST levels and hepatic inflammatory infiltration, and triggered renal damage characterized by increased relative kidney weight and tubular cell fragmentation. In the intestine, Structural alterations included shortened intestinal villi, deepened crypts, and disrupted tight junctions, accompanied by downregulated tight junction gene expression. Concurrently, OTA exposure induced intestinal oxidative stress. Mechanistically, OTA exposure reduced autophagy-related genes (SQSTM1, mTOR, and PI3K) while upregulating LC3B, ATG5, and BECN1, and triggered ferroptosis via increased jejunal mucosal iron accumulation, elevated ACSL4 and PTGS2 mRNA levels, and reduced GPX4, SLC7A11, and FTH1 expression. Additionally, 16S rRNA analysis revealed OTA changed the microbiota composition, marked by enrichment of g__norank_f__<em>Muribaculaceae</em>, g__<em>Alloprevotella</em>, g__<em>Alistipes</em>, and g__<em>Bacteroides</em>. Altogether, these findings indicated that OTA impaired the intestinal barrier resulting in intestinal injury, involving modulation of autophagy, ferroptosis, and microbial composition, while also inducing concurrent hepatorenal toxicity.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106007"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bordetella hinzii isolated from samples of commercially available raw pet food for cats in the United Kingdom (SHORT COMMUNICATION)","authors":"Genever Morgan ,&nbsp;Isabelle Kingsley ,&nbsp;Jenny Llewelyn ,&nbsp;Sam Haldenby ,&nbsp;Nicola Williams ,&nbsp;Dorina Timofte","doi":"10.1016/j.rvsc.2025.106024","DOIUrl":"10.1016/j.rvsc.2025.106024","url":null,"abstract":"<div><div>Raw meat diets for pets are frequently contaminated with potentially pathogenic and zoonotic bacteria<em>.</em> Here we present the first report, to our knowledge, of <em>Bordetella hinzii</em>, a respiratory pathogen of poultry and rare but emerging opportunistic respiratory pathogen in humans, detected incidentally in commercially available frozen pre-prepared raw meat diets for cats. <em>B. hinzii</em> was isolated from five samples of raw meat diets, from two separate brands, purchased from a retailer in the United Kingdom. Samples were a single-protein source and comprised chicken, beef, and rabbit. Whole genome sequencing indicated the presence of antimicrobial resistance genes with potential to confer resistance to multiple drug classes, including classes of highest priority critically important antibiotics. Whilst this study was limited by a small dataset and additional research surrounding the risks of transmission of <em>B. hinzii</em> is required, this novel finding indicates a further potential risk associated with the provision of raw meat diets for pets, particularly for the immunocompromised, that warrants further investigation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106024"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of otological examination techniques in captive Leopardus guttulus","authors":"Maria C.A. Martini ,&nbsp;Ana L.R. Marques ,&nbsp;Marina Marangoni ,&nbsp;Gabrielle C. Freitas ,&nbsp;Gentil F. Golçalves ,&nbsp;Zalmir S. Cubas ,&nbsp;Wanderlei de Moraes ,&nbsp;Fabíola Dalmolin ,&nbsp;Tatiana Champion","doi":"10.1016/j.rvsc.2025.106028","DOIUrl":"10.1016/j.rvsc.2025.106028","url":null,"abstract":"<div><div>This study evaluated the use of conventional otoscopy, digital otoscopy, and video-otoscopy for the examination of the external ear canal and tympanic membrane in 14 southern tiger cats (<em>Leopardus guttulus</em>). While traditional otoscopic methods did not allow detailed visualization of the epithelium or cerumen, the video-otoscopy technique provided superior image magnification, enabling better identification of anatomical structures, including vascularization of the tympanic membrane. However, mild bleeding was observed in two individuals, likely due to overheating of the xenon light source. Anatomically, the auricle, external acoustic meatus, and tympanic membrane of L. <em>guttulus</em> were similar to those of domestic cats, with a cartilaginous prominence hindered access to the horizontal ear canal in some cases. Cytological analysis revealed desquamated epithelial cells in all individuals, and <em>Malassezia</em> spp. was identified in one animal, highlighting the relevance of fungal monitoring in captive wildlife. Bacterial cultures revealed the presence of coagulase-negative Gram-positive cocci suggestive of <em>Staphylococcus</em> spp. in three samples, with minimal antibiotic resistance. Two older animals presented small, benign-appearing masses near the tympanic membrane. Overall, video-otoscopy was the most effective method for otological examination, offering high-definition imaging and documentation capabilities, although its use requires caution to prevent thermal injury.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106028"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of selected phytogenic feed additives against Ascaris suum infection in pigs","authors":"Amalie C. Pedersen ,&nbsp;Geng Pan ,&nbsp;Helena Mejer ,&nbsp;Thibaut Chabrillat ,&nbsp;Simon Guillaume ,&nbsp;Sylvain Kerros ,&nbsp;Stig M. Thamsborg ,&nbsp;Andrew R. Williams","doi":"10.1016/j.rvsc.2025.105994","DOIUrl":"10.1016/j.rvsc.2025.105994","url":null,"abstract":"<div><div>Gastrointestinal nematodes are a serious problem in pig production, and control relies mainly on the use of synthetic anthelmintics. Alternative control options would be of high value to pig producers. In this study, we tested three different feed supplements for their anthelmintic properties using <em>in vitro</em> assays and an acute, controlled 15-day infection study with <em>Ascaris suum</em> in growing pigs. <em>In vitro</em> assays showed dose-dependent activity against <em>A. suum</em> for all three supplements. <em>In vivo</em>, <em>A. suum</em> larvae numbers were reduced by 57.9 % and 58.0 % by two of the supplements (containing garlic/areca and essential oils, respectively), but these differences were not statistically significant when compared to the control group (<em>p</em> = 0.13 and 0.09, respectively). Further large-scale cohort studies are required to confirm whether the tested feed supplements could act as alternative options to control <em>A. suum</em> infections.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 105994"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145645844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine oviduct-specific glycoprotein is modulated by hormones and sperm cells","authors":"Pedro Pinto-Bravo ,&nbsp;Maria Rosa Rebordão ,&nbsp;Ana S. Amaral ,&nbsp;Anna Szóstek-Mioduchowska ,&nbsp;Carina Fernandes ,&nbsp;António M. Galvão ,&nbsp;Elisabete Silva ,&nbsp;Joana Alpoim-Moreira ,&nbsp;Rosário P. Roberto da Costa ,&nbsp;Dariusz J. Skarzynski ,&nbsp;Graça M Ferreira-Dias","doi":"10.1016/j.rvsc.2025.106022","DOIUrl":"10.1016/j.rvsc.2025.106022","url":null,"abstract":"<div><div>Oviduct glycoprotein 1 (OVGP1) is a key protein involved in oviductal functions. β-estradiol (E₂) and progesterone (P₄), oxytocin (OXT) and tumor necrosis factor-α (TNFα) modulate the equine oviduct function, through prostaglandin regulation. The objective was to evaluate OVGP1 expression within each equine oviduct segment (infundibulum, ampulla isthmus), throughout the estrous cycle. The <em>in vitro</em> effect of (i) E₂, P₄, OXT, TNFα; and (ii) spermatozoa, on oviduct OVGP1 transcription and secretion was studied. Gene transcription was assessed by real-time PCR; protein expression by western blot; and protein production by enzyme immunoassay. <em>OVGP1</em> mRNA increased in the ampulla, in the early-luteal phase (<em>P</em> &lt; 0.05). OVGP1 protein expression increased in the follicular phase, in all portions (<em>P</em> &lt; 0.05). A temporal desynchronization between transcription and protein synthesis might maintain oviduct function. In ampulla explants, OXT and TNFα up-regulated <em>OVGP1</em> transcripts in follicular phase; E₂ in early-luteal phase; and P₄ in mid-luteal phase (<em>P</em> &lt; 0.05). OXT and TNFα effect on OVGP<em>1</em> transcripts might be ascribed to prostaglandin modulation. Oviductal endogenous E₂ in follicular phase, could prime E₂ stimulation of <em>OVGP1</em> transcripts in early-luteal phase. The stimulatory effect of P₄ on <em>OVGP1</em> transcripts may modulate early embryogenesis. OVGP1 <em>in vitro</em> production was not dependent of E₂, P₄, OXT or TNFα treatments. Sperm cells, either in direct or indirect contact with oviduct explants, up-regulated OVGP1 production, in the isthmus (<em>P</em> &lt; 0.05). These data suggest that OVGP1 modulates sperm and mare's oviduct cross-talk and may play an important role in improving assisted reproductive technologies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106022"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145768913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidimensional impacts of Achyranthes bidentata polysaccharide supplementation on weaned piglets: From nutrient metabolism to systemic antioxidant enhancement","authors":"Xiaofeng Li ,&nbsp;Jing Li ,&nbsp;Bo Zhang ,&nbsp;Bing Yang","doi":"10.1016/j.rvsc.2025.106020","DOIUrl":"10.1016/j.rvsc.2025.106020","url":null,"abstract":"<div><div>This study investigated the dose-dependent effects of <em>Achyranthes bidentata</em> polysaccharides (ABPS) on physiological parameters in weaned piglets. Thirty-six piglets (25 ± 1 days old) were randomly assigned to three dietary treatments: control (0 mg/kg ABPS), low-dose (800 mg/kg), and high-dose (1600 mg/kg). On day 28 post-treatment, blood samples were collected and analyzed for mineral balance, liver function, metabolic profiles, immune parameters, and antioxidant capacity. Results showed that high-dose ABPS significantly increased serum phosphorus (<em>P</em> &lt; 0.05) without affecting calcium, chloride, or potassium levels. Low-dose ABPS elevated serum iron while reducing ceruloplasmin levels (<em>P</em> &lt; 0.05). High-dose ABPS significantly decreased activities of hepatic enzymes (glutamic pyruvic transaminase, 5′-nucleotidase, alkaline phosphatase) (<em>P</em> &lt; 0.05). Nutritionally, high-dose ABPS reduced blood urea nitrogen, creatinine, triglycerides, and LDL cholesterol, while low-dose ABPS lowered total cholesterol (<em>P</em> &lt; 0.05). Immunologically, low-dose ABPS enhanced IgM, IgG, lysozyme, and lactate dehydrogenase levels (P &lt; 0.05), with both doses reducing serum lactate. Antioxidant effects demonstrated dose dependency: high-dose ABPS upregulated superoxide dismutase and glutathione peroxidase activities, while both doses improved total antioxidant capacity and catalase activity while reducing malondialdehyde levels (<em>P</em> &lt; 0.05). In conclusion, ABPS, particularly at 1600 mg/kg, enhances mineral homeostasis, liver function, lipid metabolism, immune response, and oxidative stress resistance in weaned piglets, demonstrating its potential as a functional feed additive to alleviate post-weaning stress.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 106020"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of phytogenic additive and sodium monensin on broiler gut microbiota and immune response to Eimeria infection","authors":"T.B. Stefanello ,&nbsp;K.M. Cardinal ,&nbsp;C. Orso ,&nbsp;C.H. Franceschi ,&nbsp;J.P. Silva ,&nbsp;M.B. Mann ,&nbsp;A.P.M. Varela ,&nbsp;J. Severo ,&nbsp;J. Frazzon ,&nbsp;A.M.L. Ribeiro","doi":"10.1016/j.rvsc.2025.105989","DOIUrl":"10.1016/j.rvsc.2025.105989","url":null,"abstract":"<div><div>This study evaluated the effect of Sodium Monensin or Phytogenic additive (based on <em>Acacia concinna</em> and <em>Saccharum officinarum</em>) on the gut microbiota and immune response of broiler chickens infected with <em>E. tenella</em>, <em>E. acervulina</em>, and <em>E. maxima</em>. A total of 120 male broiler were divided in 3 treatments: Control group (without infection and additives); Ionophore group (sodium monensin, 100 ppm + <em>Eimeria</em> challenged) and Phytogenic group (poly-herbal product at 750 ppm + <em>Eimeria</em> challenged). At 28 days, the intestinal content of four birds per treatment was collected for microbiota analysis by Miseq Illumina platform. Samples of jejunum-ileum were collected for mRNA gene transcription reactions using StepOnePlus™ Real-Time PCR System. The intestinal microbiota was dominated by members of the phylum Firmicutes, regardless of treatment. Phytogenic group had a higher relative proportion of Firmicutes in the ileum-jejunum, and a higher proportion of Bacteroidetes in the cecum, while Ionophore group exhibited a higher relative proportion of Proteobacteria and Cyanobacteria. The Phytogenic group enhanced the relative abundances of Lactobacillaceae, Bacteroidaceae, and Peptostreptococcaceae, while monensin enhanced Lactobacillaceae, Rikenellaceae, Bacteroidaceae, and Clostridiaceae. Under a coccidiosis challenge, the ionophore and phytogenic additives modulated the immune system by increasing <em>IL-6</em> and <em>MUC13</em> gene transcription compared to the Control group. The phytogenic product, a mixture composed by <em>A. concinna</em> and <em>S. officinarum,</em> and sodium monensin enhanced the abundance of important bacterial groups for the establishment of a favorable intestinal microbiota in the face of an intestinal challenge. This study found that both phytogenic additives and sodium monensin altered gut microbiota composition and enhanced immune responses in broilers infected with <em>Eimeria</em> spp.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"199 ","pages":"Article 105989"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocular abnormalities, tear production, and intraocular pressure findings in Italian holstein calves during their first four weeks of life","authors":"Irene Nocera ,&nbsp;Francesca Bonelli ,&nbsp;Samanta Nardi ,&nbsp;Luca Turini ,&nbsp;Grace Ann West ,&nbsp;Caterina Puccinelli ,&nbsp;Giovanni Barsotti","doi":"10.1016/j.rvsc.2025.105983","DOIUrl":"10.1016/j.rvsc.2025.105983","url":null,"abstract":"<div><div>The study evaluated ocular abnormalities, Schirmer tear test 1 (STT 1) and intraocular pressure (IOP) values in healthy Italian-Holstein calves from birth to 4 weeks old, and compared STT 1 and IOP with those in adult bovines. Fifty-four calves underwent ophthalmic examination, and STT 1 and IOP assessment were performed within 48 h from birth, then weekly for the first weeks of age. Ophthalmic examination, STT 1 and IOP were assessed in calves' dams. Results for ocular abnormalities were shown as frequency and percentage. Differences in STT 1 and IOP were evaluated according to age, gender, and weight with ANOVA test. A <em>t</em>-test was used to compare calves' STT 1 and IOP at each measurement time with values in the calves' dams. Ocular lesions recorded were: 50 % subconjunctival haemorrhages, 25 % retinal haemorrhages, and 25 % cataract. At birth, remnants of the hyaloid system (51/51, 100 %) and <em>tunica vasculosa lentis</em> (49/51, 96 %) were detected, which regressed completely by the 16th week and 6th week, respectively. No statistical differences were found related to STT 1 and IOP. Statistical differences were observed between IOP as well as STT 1 in calves' dams and calves, for all time points (<em>p</em>-value &lt;0.0001), with higher values in adult bovines. Ocular haemorrhages were the main ocular lesion observed, and cataract was the only congenital ocular abnormality diagnosed. At birth, calf STT 1 and IOP did not change during the first month of life, and adult bovines showed higher IOP and STT 1 compared to neonatal calves.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105983"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative histologic survey and transcriptomic investigation into canine prostate carcinoma","authors":"Nathan K. Hoggard ,&nbsp;Said M. Elshafae ,&nbsp;Nigel A. Daniels ,&nbsp;Jonathan A. Young ,&nbsp;Chris Premanandan ,&nbsp;John B. Echols ,&nbsp;Darshan S. Chandrashekar ,&nbsp;Blake E. Hildreth III ,&nbsp;Michael C. Haffner ,&nbsp;Thomas J. Rosol","doi":"10.1016/j.rvsc.2025.105981","DOIUrl":"10.1016/j.rvsc.2025.105981","url":null,"abstract":"<div><div>Dogs share features in prostate gland anatomy, physiology, and pathology with men. However, human and canine prostate carcinoma (PC) have histologic and molecular differences. Particularly, the histogenesis of canine PC (cPC) is unclear. This study investigated the origin of cPC using histopathology and transcriptomics with comparison to men. Prostate glands retrospectively and prospectively collected from 445 dogs (approximately 95 % autopsy samples) were surveyed for early carcinomas and preneoplastic lesions, particularly high-grade prostatic intraepithelial neoplasia (HGPIN) due to its role in the pathogenesis of PC in men. Lineage gene signatures defining prostate luminal epithelium and urothelium were identified for inter- and intraspecies RNA-sequencing comparisons, including between cPC and canine urinary bladder urothelial carcinoma (UC). Postmortem prostate lesion frequencies were similar to previously reported canine studies. Intraductal/intra-acinar growth (31/35; 88.6 %) was common in representative samples of cPC. Prostate epithelial changes consistent with HGPIN in men were not observed. Proliferative lesions and early carcinomas were rare (7/445; 1.6 %). Patterns in prostate and urothelium marker gene expression signatures differed between human and canine PC. Compared to non-neoplastic prostate gland, cPC had significantly decreased prostate-specific and increased urothelium gene signatures. The results suggest many cases diagnosed as cPC are UC or have urothelial differentiation and thus differ from PC in men, with important implications for canine tumor classification and translational studies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"198 ","pages":"Article 105981"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}