Research in veterinary science最新文献

{"title":"Attenuated Salmonella Typhimurium expressing chimeric antigen from Leptospira as a recombinant vaccine candidate against leptospirosis","authors":"Domitila Brzoskowski Chagas ,&nbsp;Francisco Denis Souza Santos ,&nbsp;Natasha Rodrigues de Oliveira ,&nbsp;Eugenia Gutiérrez Noble ,&nbsp;Mara Andrade Colares Maia ,&nbsp;Amilton Clair Pinto Seixas Neto ,&nbsp;Jady Duarte Nogueira ,&nbsp;Lucia Yim ,&nbsp;Maria Moreno ,&nbsp;Jose Alejandro Chabalgoity ,&nbsp;Thaís Larré Oliveira Bohn ,&nbsp;Odir Antônio Dellagostin","doi":"10.1016/j.rvsc.2025.105802","DOIUrl":"10.1016/j.rvsc.2025.105802","url":null,"abstract":"<div><div>Leptospirosis is a significant zoonotic disease caused by pathogenic spirochetes of the genus <em>Leptospira</em>. Attenuated <em>Salmonella</em> strains represent a promising vector for the development of safe and effective vaccines capable of eliciting a specific immune response. A chimeric <em>Leptospira</em> antigen (rQ1), previously described and based on the gene sequences of <em>lipL32</em>, <em>lemA</em>, and <em>ligAni</em>, was cloned into the pTECH2 vector for antigen expression in <em>Salmonella</em> Typhimurium LVR01. Hamsters were vaccinated orally (OR) and intramuscularly (IM) with 2 × 10^7 CFU of <em>S.</em> Typhimurium LVR01 carrying pTECH2/rQ1, pTECH2 alone, or PBS as a control. Vaccinations were administered twice, with a 14-day interval. Following OR and IM administration, serum samples were collected, and IgG antibodies against rQ1 were measured using indirect ELISA. The results showed that after the second immunization, IgG antibody levels in hamsters immunized IM with pTECH2/rQ1 were significantly higher (<em>P</em> &lt; 0.0001) than in the control groups (PBS and pTECH2). Isotype analyses revealed significant levels of IgG2 (<em>P</em> &lt; 0.05) in the group vaccinated IM with pTECH2/rQ1. However, the attenuated <em>S.</em> Typhimurium vaccine did not confer protective efficacy against homologous challenge in the hamsters immunized with pTECH2/rQ1. Further studies are necessary to explore this novel recombinant attenuated <em>S.</em> Typhimurium LVR01 vaccine and to further characterize the immune mechanisms involved.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105802"},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of vaginal microflora in healthy postpartum cows and cows with incomplete uterine involution by 16S rRNA gene sequencing","authors":"Xiaofeng Li ,&nbsp;Xiaoli Yang ,&nbsp;Ziming Ma ,&nbsp;Min Liu ,&nbsp;Yun Ma ,&nbsp;Xingang Dan","doi":"10.1016/j.rvsc.2025.105813","DOIUrl":"10.1016/j.rvsc.2025.105813","url":null,"abstract":"<div><div>This study aimed to uncover the characteristics of vaginal microflora alterations between healthy postpartum cows and those with incomplete uterine involution. Vaginal swabs were collected from both healthy cows and those with incomplete uterine involution at 5, 10, 15, 20, 30, and 40 days postpartum. 16S rRNA sequencing was employed to analyze the features, changing trends, and differential species of the vaginal microflora between the two groups. The outcomes indicated that during the early postpartum phase, the diversity of vaginal microflora in cows with incomplete uterine involution was significantly lower than that in the healthy group. At the phylum level, the abundances of the <em>Fusobacteriota</em> and <em>Bacteroidota</em> in the vaginas of cows with incomplete uterine involution were significantly higher than those in healthy cows at 5 days postpartum. <em>Fusobacteriota</em> also showed significantly higher abundance in the vaginas of cows with incomplete uterine involution at 20 days postpartum. Furthermore, <em>Synergistota</em> was significantly higher in the vaginas of cows with incomplete uterine involution at 30 days postpartum, while significantly lower at 5 days postpartum. In contrast, the abundance of vaginal <em>Firmicutes</em> was significantly lower in cows with incomplete uterine involution at 5, 15, and 20 days postpartum. At the genus level, the abundances of <em>Fusobacterium</em>, <em>Porphyromonas</em>, and <em>Prevotella</em> in the vaginas of cows with incomplete uterine involution were significantly higher than in the healthy group during the postpartum period 5–20 days. Additionally, the abundances of <em>Anaerosalibacter</em>, unidentified_<em>Clostridia_vadinBB60_group</em>, <em>Rikenellaceae</em>_RC9_<em>gut_group</em>, <em>UCG_005, Ureaplasm</em>a, and <em>UCG_010</em> showed significant differences between the healthy cows and cows with incomplete uterine involution. These differential vaginal microflora might serve as potential microbial targets for the early detection of incomplete uterine involution in postpartum dairy cows. Nevertheless, current research has yet to establish a correlation between the changes in the diversity and abundance of vaginal bacterial and postpartum uterine involution in dairy cows.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105813"},"PeriodicalIF":2.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-directed mutagenesis of transferrin binding protein B enhances immunogenicity and protective efficacy against Bibersteinia trehalosi","authors":"Zhihao Yan,&nbsp;Xingyi Ma,&nbsp;Wenyao Long,&nbsp;Rongyue Yang,&nbsp;Jiaquan Zhu,&nbsp;Chenyu Wei,&nbsp;Xin Yu,&nbsp;Ling Gan,&nbsp;Jianhua Guo","doi":"10.1016/j.rvsc.2025.105812","DOIUrl":"10.1016/j.rvsc.2025.105812","url":null,"abstract":"<div><div><em>Bibersteinia trehalosi (B. trehalosi)</em> is a gram-negative bacterial pathogen that causes respiratory diseases in goats. Its surface transferrin receptor, transferrin binding protein B(TbpB), is an ideal vaccine target as its expression is essential for colonization of the upper respiratory tract. Previous studies have shown that mutations that reduce affinity of TbpB toward transferrin can significantly enhance the immunogenicity of TbpB. In this study, amino acid residues that are key to the function of TbpB were determined using 3-D modelling of the protein structure. The key residues were mutated to alanine via site-directed mutagenesis, and a solid-phase binding assay was carried out to assess their binding activities to goat transferrin. Mice were immunized thrice with TbpB antigens. TbpB-specific antibody titers and bactericidal activity were evaluated. The mice were challenged with the pathogen after immunization, and histopathological analysis was performed after euthanasia. The results showed that the L156A mutant TbpB exhibited the highest serum antibody levels and maintained a 48.11 % bactericidal rate even after 12 h of co-incubation with <em>B. trehalosi</em>. Histopathological examination revealed that mice immunized with L156A TbpB mutant had the mildest pulmonary lesions. Altogether, these findings suggest that the leucine residue at position 156 of <em>B. trehalose</em> TbpB is crucial for transferrin binding and induced a stronger immune response and provided better protection.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105812"},"PeriodicalIF":2.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of heat stress on intestinal histopathology and cellular immune response in chickens infected with Eimeria tenella","authors":"Nguyen Thi Hoa,&nbsp;Le Dinh Phung,&nbsp;Van Nguyen Chao,&nbsp;Pham Hoang Son Hung,&nbsp;Tran Nguyen Thao,&nbsp;Tran Thi Na,&nbsp;Ho Thi Dung","doi":"10.1016/j.rvsc.2025.105811","DOIUrl":"10.1016/j.rvsc.2025.105811","url":null,"abstract":"<div><div>This study was conducted to investigate the pathological characteristics and cellular immune response of chickens infected with <em>Eimeria tenella</em> under heat stress conditions. A total of 140 chicks were randomly assigned to four groups: a control group (CON), a group infected with <em>E. tenella</em> (Et), a heat stress group (HS), and a heat stress group infected with <em>E. tenella</em> (HS-Et). The HS and HS-Et groups were maintained at 38 ± 1 °C from 1 to 24 days old, while chickens in the CON and Et group were raised in thermoneutral conditions. At 14 days old, chicks in the Et and HS-Et groups were orally inoculated with 2 × 10⁴ sporulated <em>E. tenella</em> oocysts. Fecal samples were collected between 4 and 10 days post-inoculation (dpi) to quantify oocyst shedding. Chickens were sacrificed at 0, 5, and 10 dpi for histopathological examination and cytokines gene (IL-12, IL-1β, IFN-γ, IL-4, IL-10, IL-13) expression analysis using qRT-PCR. The results showed that although fecal oocyst shedding was significantly lower in the HS-Et group compared to the Et group (<em>P</em> &lt; 0.05), the histopathological lesion scores were highest in this group at 10 dpi. There were no significant differences in Th1 and Th2 cytokines expression levels among the three infected groups, except for IL-12. Notably, IL-12 expression was significantly higher in the HS-Et group than in the other three groups. These findings suggest that heat stress suppresses the growth of <em>E. tenella</em> but exacerbates tissue damage by enhancing inflammation and activating the Th1 response via the IL-12 pathway.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105811"},"PeriodicalIF":2.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of avian pathogenic Escherichia coli (APEC) and antimicrobial resistance in layer parent stock in Bangladesh: Insights into phenotypic and genotypic profiles","authors":"Md. Nowshad Atiq ,&nbsp;Md. Saiful Islam ,&nbsp;Md. Ashek Ullah ,&nbsp;Nirab Chakroborty ,&nbsp;Raihana Islam ,&nbsp;Md. Liton Rana ,&nbsp;Farhana Binte Ferdous ,&nbsp;Md. Abdus Sobur ,&nbsp;Mohammad Ferdousur Rahman Khan ,&nbsp;Md. Tanvir Rahman","doi":"10.1016/j.rvsc.2025.105810","DOIUrl":"10.1016/j.rvsc.2025.105810","url":null,"abstract":"<div><div>Avian pathogenic <em>Escherichia coli</em> (APEC) poses a major threat to poultry health and productivity, particularly in layer parent stock, resulting in substantial economic losses. This study aimed to determine the occurrence of APEC along with their virulence and resistance profile in the layer parent stock. A total of 89 samples, including cloacal swabs from 36 live birds and liver swabs from 53 dead birds, were collected. <em>E. coli</em> was isolated using culture, biochemical, and PCR methods. APEC-related virulence genes were detected via PCR, while antimicrobial susceptibility was evaluated using the disc diffusion method and PCR. The overall prevalence of <em>E. coli</em> was 82 %, with similar detection rates in live (83.3 %) and dead birds (81.1 %). In PCR, APEC-associated gene <em>fimC</em> was detected in 95.9 % of the isolates, <em>papC</em> in 36.9 %, and <em>iucD</em> in 15.1 %, with no significant difference between those from live and dead birds. All isolates were multidrug-resistant, where all <em>E. coli</em> isolates from both live and dead birds were resistant to ampicillin (100 %), with high resistance to cefotaxime (live: 100 % vs. dead: 93 %), tetracycline (80 % vs. 88.4 %), nalidixic acid (100 % vs. 62.8 %), streptomycin (66 % vs. 39.5 %), norfloxacin (40 % vs. 20.9 %), and chloramphenicol (20 % vs. 25.6 %). Moreover, genes conferring resistance to tetracyclines [<em>tetA</em> (live: 10/10 vs. dead: 10/10) and <em>tetB</em> (1/10 vs. 10/10)] and beta-lactams [<em>bla</em>_TEM (10/10 vs. 10/10), <em>bla</em>_CTX-M (1/10 vs. 4/10), and <em>bla</em>_SHV (2/10 vs. 0/10)] were detected in isolated <em>E. coli</em>. The results recommend periodic surveillance and policy interventions to mitigate health risks and economic losses.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105810"},"PeriodicalIF":2.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bacteriophage supplementation on performance, gut health and blood biochemistry in broilers challenged with necrotic enteritis","authors":"Muhammad Muneeb ,&nbsp;Ehsaan Ullah Khan ,&nbsp;Saima ,&nbsp;Muhammad Suleman ,&nbsp;Mian Muhammad Awais ,&nbsp;Sohail Ahmad","doi":"10.1016/j.rvsc.2025.105809","DOIUrl":"10.1016/j.rvsc.2025.105809","url":null,"abstract":"<div><div>Necrotic enteritis (NE), primarily caused by <em>Clostridium perfringens</em> (CP) type G, poses a significant threat to broiler health and performance, especially in antibiotic-restricted production systems. Bacteriophage therapy is emerging as a promising alternative to antibiotics for controlling enteric pathogens in poultry, including CP. However, a critical knowledge gap exists in specifically determining the in vivo effects of bacteriophages combined with probiotics against CP strains associated with NE in broilers under practical conditions. Therefore, this study aimed to evaluate the efficacy of a bacteriophage preparation (PathoGone™-DWA; containing <em>Bacillus subtilis</em> + Phages cocktail) administered via drinking water in NE-challenged broilers. In total, 576 one-day-old (Ross-308) male chicks were randomly assigned to four treatments (6 pens of 24 birds): (1) Negative Control (NC; uninfected + basal diet), (2) Positive Control (PC; infected + basal diet), (3) Low Dose Bacteriophage (LD-BP; infected +0.6 g/pen/day), and (4) High Dose Bacteriophage (HD-BP; infected +1.2 g/pen/day). Birds were orally predisposed with 10× coccidia vaccine (1 mL/bird) on day 15, followed by oral inoculation with a pathogenic (NetB-positive) field strain of <em>C. perfringens</em> type G (1 mL; 1 × 10⁸ CFU/mL/ bird) on day 19 and 20. Bacteriophage supplementation significantly improved feed intake, body weight gain, and feed conversion ratio (<em>P</em> &lt; 0.05), while reducing intestinal lesion scores and mortality. Jejunal histomorphology and hematological indices depicted a notable restoration (P &lt; 0.05) of NE-associated gut damage and blood abnormalities. Serum biochemistry also showed marked improvement (P &lt; 0.05) in protein, glucose, lipid profile, liver enzymes, and renal markers. In conclusion, bacteriophage supplementation (PathoGone™-DWA) significantly improved performance metrics, mitigated intestinal damage, and restored hematological and biochemical disturbances in broilers challenged with NetB-positive CP, highlighting its efficacy as a targeted, antibiotic-free intervention against NE.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105809"},"PeriodicalIF":2.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of enflicoxib efficacy in the control of postoperative pain and inflammation in dogs undergoing orthopaedic surgery. A pilot randomised clinical trial","authors":"Marta Salichs ,&nbsp;Antonio Arcas ,&nbsp;Josep Homedes ,&nbsp;Cristina Costa-Farré","doi":"10.1016/j.rvsc.2025.105804","DOIUrl":"10.1016/j.rvsc.2025.105804","url":null,"abstract":"<div><div>Postoperative pain is most effectively managed pre-emptively and enflicoxib characteristics make it a good candidate to control it with a single administration. To assess the efficacy of enflicoxib in the control of postoperative pain and inflammation associated with orthopaedic surgery, twenty-eight dogs were randomised to receive 8 mg/kg enflicoxib (<em>n</em> = 14) orally the day before surgery, or 0.2 mg/kg meloxicam (n = 14) subcutaneously at induction and orally at 0.1 mg/kg daily every 24 h for seven days. Veterinarians assessed efficacy with the Glasgow Composite Pain Scale Short-Form (GCPS-SF) at 1.5, 3, 5, 8, 24 and 168 h after surgery. Visual analog scales (VAS) were also used to assess pain at rest, pain at palpation and inflammation. Enflicoxib showed to be non-inferior to meloxicam in the GCPS-SF total scores, at each time point, and the area under the curve (GCPS-SF AUC) for the first 24 h. No differences were observed in VAS scores at any time point after surgery or the global efficacy as assessed by the veterinarians, or the dog's wellbeing assessed by the owners. Enflicoxib administered 24 h before surgery is efficacious and safe for the control of postoperative pain associated with orthopaedic surgery. One single dose would control postoperative pain and inflammation for one whole week. The results need further confirmation in larger sample size studies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105804"},"PeriodicalIF":2.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructure of the intestinal canal in healthy turkeys (Meleagris gallopavo) using light and scanning electron microscopy","authors":"Fatma Işbilir ,&nbsp;İhsan Işbilir ,&nbsp;Senem Esin Yavaş ,&nbsp;Barış Can Güzel","doi":"10.1016/j.rvsc.2025.105805","DOIUrl":"10.1016/j.rvsc.2025.105805","url":null,"abstract":"<div><div>This study investigated the ultrastructural and histological features of the intestinal canal in healthy adult turkeys (<em>Meleagris gallopavo</em>) using light and scanning electron microscopy. Tissue samples from the duodenum, jejunum, ileum, and caecum were analyzed to characterize the organization of the intestinal wall. All segments exhibited the classical four-layered structure: tunica mucosa, submucosa, muscularis externa, and serosa. Significant morphological differences were identified among the intestinal segments. Villi in the duodenum showed thick, leaf-like or finger-like forms depending on location, whereas jejunal villi were thinner and elongated. Ileal villi presented a knob-like appearance, while caecal villi changed from finger-shaped at the base to leaf-shaped at the apex. Lieberkühn crypt depth and the density of Paneth cells increased from the duodenum to the ileum. Importantly, abundant lymphoid structures—including diffuse lymphoid cells, Peyer's patches, and caecal tonsils—were prominent in the lamina propria and submucosa, especially in the ileum and caecum. These findings emphasize the regional specialization of the turkey intestine for absorption and immune defense, contributing valuable reference data for future avian gastrointestinal studies and comparative histology.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105805"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical profiling of VISTA expression in canine mammary tumors: A pilot study for immunotherapeutic targeting","authors":"Muhammed Ali Yamac ,&nbsp;Leyla Elif Ozgü Ayozger ,&nbsp;Volkan Ipek","doi":"10.1016/j.rvsc.2025.105807","DOIUrl":"10.1016/j.rvsc.2025.105807","url":null,"abstract":"<div><div>VISTA (V-domain Ig-containing suppressor of T cell activation) is an immune checkpoint protein that regulates the activity of T cells and is involved in immune tolerance. Although its role has been elucidated in human oncology, its significance in canine mammary tumors (CMTs) is unclear. This research was focused on investigating VISTA expression in CMTs and its correlation with histologic grade and molecular subtype. Archived formalin-fixed, paraffin-embedded mammary gland tumors taken from 26 canine patients diagnosed with malignant tumors were studied. Histopathologic classification and grading were performed using hematoxylin and eosin–stained sections. Immunohistochemical staining for estrogen receptor (ER), progesterone receptor (PR), HER2, and VISTA was done, and molecular subtypes were defined as based on ER, PR, and HER2 status. VISTA immunoreactivity was semiquantitatively evaluated in tumor cells and in inflammatory cells. VISTA expression in neoplastic cells and in cells of the inflammatory infiltrate was found. Increased tumor grade was found to have significantly increased VISTA staining intensity and overall score in tumor cells. Inflammatory cell staining differed according to grade, yet this difference was not significant. No significant difference in VISTA expression was detected in molecular subtypes. This is the initial research to shed light on VISTA expression in CMTs. Its identification within tumor cells and within cells of the infiltrate, and its correlation with tumor grade, places its importance as an immunotherapeutic and prognostic marker in mammary cancer in dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105807"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the mechanisms of ovulatory failure in dairy cows: The role of lipid metabolism","authors":"Natalia Carolina Gareis ,&nbsp;María Lucía Cattaneo-Moreyra ,&nbsp;Antonela Florencia Stassi ,&nbsp;Maria Emilia Odriozola ,&nbsp;Emmanuel Angeli ,&nbsp;Fernanda Mariel Rodríguez ,&nbsp;Hugo Héctor Ortega ,&nbsp;Natalia Raquel Salvetti ,&nbsp;Gustavo Juan Hein ,&nbsp;Florencia Rey","doi":"10.1016/j.rvsc.2025.105806","DOIUrl":"10.1016/j.rvsc.2025.105806","url":null,"abstract":"<div><div>Ovulatory failure in dairy cows can stem from various causes, including the formation of follicular cysts, which disrupt the estrous cycles and extend reproductive intervals, ultimately hindering herd management and reproductive performance. Reproductive dysfunctions are frequently associated with metabolic disorders, commonly observed in these animals. Therefore, this study aimed to evaluate key components of lipid metabolism, primarily related to β-oxidation, including AdipoR1, AdipoR2, AMPK, PPARγ, ObR, ACC1, CPT1, and ACOX1 in ovary, as well as adiponectin, leptin, triglyceride (TG) and non-esterified fatty acid (NEFA) concentrations in plasma and follicular fluid of dairy cows with induced persistent follicles, spontaneous follicular cysts and dominant control follicles. Ovary protein immunoexpression was assessed <em>in situ</em> by immunohistochemistry, plasma and follicular fluid adiponectin and leptin concentrations were quantified by ELISA, and TG and NEFA concentrations were determined by colorimetric methods. The results indicated increased protein expression of adiponectin receptors in follicles of 15 days of persistence, along with an upregulation of CPT1 expression. ACC1 expression was lower in both follicles of 15 days of persistence and in spontaneous cysts than in preovulatory follicles. Furthermore, PPARγ expression was decreased in follicles of 15 days of persistence compared to dominant control follicles. These findings indicate changes in key molecules involved in ovarian lipid metabolism, with a pronounced shift towards β-oxidation pathways and decreased lipid synthesis and lipid storage. Such metabolic disruptions may contribute to impaired ovarian function, potentially leading to ovulatory failure of the preovulatory follicle.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"193 ","pages":"Article 105806"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}