Research in veterinary science最新文献_第9页

Pathogenicity of a chicken-origin cluster 3 isolate of Tembusu virus in Pekin ducklings and Kunming mice 天布苏病毒鸡源聚群3分离株对北京雏鸭和昆明小鼠的致病性

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-28 DOI: 10.1016/j.rvsc.2025.105670

Qiong Li, Jingjing Huang, Xiaoyang Hu, Duo Peng, Yinuo Song, Dabing Zhang

{"title":"Pathogenicity of a chicken-origin cluster 3 isolate of Tembusu virus in Pekin ducklings and Kunming mice","authors":"Qiong Li, Jingjing Huang, Xiaoyang Hu, Duo Peng, Yinuo Song, Dabing Zhang","doi":"10.1016/j.rvsc.2025.105670","DOIUrl":"10.1016/j.rvsc.2025.105670","url":null,"abstract":"<div><div>Tembusu virus (TMUV) is genetically classified into three clusters. To date, information relating to the pathogenicity of TMUV cluster 3 in ducks is limited; whether TMUV cluster 3 has the potential to induce disease in mice remains unclear. Here, we evaluated the pathogenicity of a chicken-origin TMUV cluster 3 strain G (TMUV/G) in 5-day-old Pekin duck and 3-week-old Kunming mouse models by comparing with TMUV cluster 2.1 strain H (TMUV/H). TMUV/G caused neurological diseases in both ducklings and mice. Whereas TMUV/G was less virulent than TMUV/H, with more mild clinical signs and microscopic lesions and significantly lower morbidity. TMUV/G caused 10 % mortality in ducklings and failed to cause mortality in mice, in sharp contrast with 90 % mortality in ducklings and 60 % mortality in mice induced by TMUV/H. Further analysis with the mouse model revealed that TMUV/G replicated to lower levels in the central nervous system, and induced lower IFN-β, IL-6, TNF-α, and Viperin responses in the infected cerebrum. Compared with TMUV/H, TMUV/G contained a number of mutations throughout the genome and had a reduced replication efficiency in cultured hippocampal neuronal (HT22) cells. Together these findings suggest that TMUV/G displays markedly lower virulence in Pekin ducklings and Kunming mice relative to TMUV/H, indicating that different virus-host interactions exist between cluster 3 and 2.1 TMUVs. The present work contributes to the understanding of virulence phenotype of TMUV cluster 3, and will stimulate further studies on the mechanism for substantial virulence attenuation of TMUV cluster 3 relative to TMUV cluster 2.1.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105670"},"PeriodicalIF":2.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive biomarkers for monitoring intestinal health in broilers – A systematic review 肉鸡肠道健康监测的非侵入性生物标志物系统综述

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-24 DOI: 10.1016/j.rvsc.2025.105669

Paula Gabriela da Silva Pires , Kátia Maria Cardinal , Shaaban Saad Elnesr , Vanessa Peripolli , Betina Raquel Cunha dos Santos , Priscila de Oliveira Moraes

{"title":"Non-invasive biomarkers for monitoring intestinal health in broilers – A systematic review","authors":"Paula Gabriela da Silva Pires , Kátia Maria Cardinal , Shaaban Saad Elnesr , Vanessa Peripolli , Betina Raquel Cunha dos Santos , Priscila de Oliveira Moraes","doi":"10.1016/j.rvsc.2025.105669","DOIUrl":"10.1016/j.rvsc.2025.105669","url":null,"abstract":"<div><div>This systematic review aimed to identify and evaluate non-invasive biomarkers for assessing gut health in broilers, following PRISMA guidelines and using the PICO strategy. A comprehensive search was conducted in Web of Science, Scopus, and PubMed for papers written in English, Portuguese, and Spanish. After screening 1196 papers, 21 studies met the inclusion criteria and were included in the review. The studies, primarily conducted in Europe, Oceania, and North America, were published between 2003 and 2024. The review highlighted the intestinal microbiota as the most studied non-invasive biomarker, using excreta, cloacal swabs, poultry litter, and poultry dust as sample sources. Poultry litter and dust were identified as promising non-invasive alternatives for assessing gut microbiota, with excreta being the most commonly used sample type. Biomarkers like ovotransferrin, lipocalin-2, and calprotectin are under investigation for broiler gut health assessment. Cecal samples were frequently used as a gold standard for comparison. The choice of reference databases, such as SILVA and Greengenes, varied among studies, which may have influenced taxonomic classification and microbial group abundance. Methodological quality varied across studies, with a mix of observational and experimental designs. Many studies involved comparative analyses related to dietary and sanitary challenges, though inconsistencies in reporting diet composition and performance data were observed. In conclusion, many non-invasive biomarkers are under investigation for broiler gut health assessment, but challenges remain in validating these markers due to the complexity of the gut ecosystem. These challenges emphasize the need for rigorous study design, thorough data collection, and standardized protocols.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105669"},"PeriodicalIF":2.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital cerebello-cerebral cortical degeneration in a calf 小牛先天性小脑-大脑皮质变性

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-23 DOI: 10.1016/j.rvsc.2025.105652

Susumu Ohfuji

{"title":"Congenital cerebello-cerebral cortical degeneration in a calf","authors":"Susumu Ohfuji","doi":"10.1016/j.rvsc.2025.105652","DOIUrl":"10.1016/j.rvsc.2025.105652","url":null,"abstract":"<div><div>Among neurodegenerative disorders affecting neurons in the central nervous system of domestic animals is a distinct entity known as multisystem neuronal degeneration which is often associated with significant cerebellar involvement. Occurrence of such a multisystem neuronal degeneration has extremely been uncommon in the bovine species. A female Shorthorn calf clinically presented with progressive ataxic signs that had lasted since birth until her euthanasia at 4.5 months of age. Confirmed histopathologically, the cerebellum exhibited severe and mild cortical degeneration: the former was present in grossly atrophied anterior vermis and flocculonodular lobe, which demonstrated total and subtotal loss of Purkinje and granule cells, respectively; the latter, in grossly normal posterior vermis which displayed varying degrees of Purkinje cell loss, while preserving granule cells. Concurrent with these cerebellar cortical lesions were shrinkage of a few neurons in the olivary nuclei of the medulla oblongata and focal cerebral cortical degeneration comprising neuronal red cell change in the left parietal lobe. This congenital progressive neurodegenerative disorder manifesting as combined cerebellar and cerebral cortical degeneration was regarded as a new variant of multisystem neuronal degeneration in the bovine species, most likely giving rise to a functional impairment of the feedback circuit between the cerebellar and cerebral cortex. This neurodegenerative disorder should be subclassified on clinical and neuropathologic grounds as a descriptive term <em>congenital cerebello-cerebral cortical degeneration.</em></div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105652"},"PeriodicalIF":2.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive modeling based on machine learning for mapping risk areas of human sporotrichosis in southeastern Brazil 基于机器学习的预测建模，用于绘制巴西东南部人类孢子虫病的风险区域

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-22 DOI: 10.1016/j.rvsc.2025.105651

Ailton Junior Antunes da Costa , Maria Helena Franco Morais , Isadora Martins Pinto Coelho , Fernanda do Carmo Magalhães , Rafael Romero Nicolino , Marcelo Antônio Nero , Otávia Augusta de Mello , Marcos Xavier Silva

{"title":"Predictive modeling based on machine learning for mapping risk areas of human sporotrichosis in southeastern Brazil","authors":"Ailton Junior Antunes da Costa , Maria Helena Franco Morais , Isadora Martins Pinto Coelho , Fernanda do Carmo Magalhães , Rafael Romero Nicolino , Marcelo Antônio Nero , Otávia Augusta de Mello , Marcos Xavier Silva","doi":"10.1016/j.rvsc.2025.105651","DOIUrl":"10.1016/j.rvsc.2025.105651","url":null,"abstract":"<div><div>Sporotrichosis, a zoonotic mycosis with a growing public health impact, requires innovative methods to map risk areas. This study applied machine learning techniques, Artificial Neural Networks (ANN), and Decision Trees (DT) to integrate sociodemographic, epidemiological, environmental, and urban data from Contagem, Minas Gerais, Brazil. Both models exhibited high predictive capacity, with complementary performances: the ANN stood out in class discrimination (mean accuracy of 0.9106, AUC of 0.939, MSE of 0.4040, RMSE of 0.6313, R<sup>2</sup> of 0.5955), while the DT demonstrated greater consistency and lower errors (mean accuracy of 0.9185, AUC of 0.9147, MSE of 0.0695, RMSE of 0.2625, R<sup>2</sup> of 0.6285). The DT also identified key risk factors, such as the presence of parks, squares, soccer fields, positive cats, health facilities, and suburban clusters. Spatial analysis reinforced the findings, with the comparative map showing high similarity between actual and predicted data: of the 884 census sectors, 221 (25 %) recorded positive human cases against 219 (24.78 %) predicted by the ANN. These results highlight the potential of the techniques used to optimize the monitoring and control of sporotrichosis, enriching the understanding of its epidemiology and providing robust instruments for developing more effective control strategies, promoting significant advances in public health and animal welfare.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105651"},"PeriodicalIF":2.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the inflammatory response in horses undergoing gastric ulceration using salivary ADA and S100A12 as biomarkers 使用唾液ADA和S100A12作为生物标志物评估胃溃疡马的炎症反应

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-22 DOI: 10.1016/j.rvsc.2025.105667

M. Matas-Quintanilla , C.A.S. Bonifay , L. Whitacre , I.R. Ipharraguerre , A.M. Gutiérrez

{"title":"Assessing the inflammatory response in horses undergoing gastric ulceration using salivary ADA and S100A12 as biomarkers","authors":"M. Matas-Quintanilla , C.A.S. Bonifay , L. Whitacre , I.R. Ipharraguerre , A.M. Gutiérrez","doi":"10.1016/j.rvsc.2025.105667","DOIUrl":"10.1016/j.rvsc.2025.105667","url":null,"abstract":"<div><div>The inflammatory status in horses with gastric ulcers was monitored by two inflammatory biomarkers, S100A12 and adenosine deaminase (ADA), using saliva samples from healthy and horses with gastric ulcers. Two trials were developed: an experimental trial, in which gastric ulceration was induced in 8 horses by feed deprivation, and a clinical trial, in which 20 horses without any symptomatology and 37 with symptoms compatible with gastric ulcers were used. S100A12 and ADA levels were measured in the saliva samples of all horses using previously validated assays. In both trials, horses with gastric ulcers had higher levels of S100A12 and ADA than healthy horses, demonstrating that inflammation is involved in the pathophysiology of gastric ulcers in horses. An intense reaction of ADA with a moderate increase of S100A12 was detected in the experimental trial, while different intensities in the inflammatory biomarkers were observed in clinical conditions when the ulcers were divided by gastric localization in glandular (EGGD) and squamous (ESGD). Since ADA has been shown to be released early in the inflammatory process and S100A12 is released later, by measuring them together we could more accurately assess the inflammatory process in equine gastric ulcers. Furthermore, a strong association was found between ADA and S100A12 (correlation coefficient of 0.84) in the experimental trial, while it was low-moderate (correlation coefficient <u>of 0.39</u>) in the clinical trial. Therefore, it could be concluded that both salivary biomarkers provide valuable information about the inflammation involved in gastric ulcers of horses and the state of the process.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105667"},"PeriodicalIF":2.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered expression of collagen gene family members and its epigenetic background in equine Sarcoids 马肉瘤中胶原基因家族成员表达的改变及其表观遗传背景

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-21 DOI: 10.1016/j.rvsc.2025.105656

Klaudia Pawlina-Tyszko , Ewelina Semik-Gurgul , Przemysław Podstawski , Weronika Herc , Maciej Witkowski , Katarzyna Ropka-Molik

{"title":"Altered expression of collagen gene family members and its epigenetic background in equine Sarcoids","authors":"Klaudia Pawlina-Tyszko , Ewelina Semik-Gurgul , Przemysław Podstawski , Weronika Herc , Maciej Witkowski , Katarzyna Ropka-Molik","doi":"10.1016/j.rvsc.2025.105656","DOIUrl":"10.1016/j.rvsc.2025.105656","url":null,"abstract":"<div><div>Alterations in the genes involved in the creation of the extracellular matrix (ECM) were observed in our earlier transcriptome studies of sarcoids and their cell culture model. For a complete characterization of the underlying molecular pathways, it is imperative to comprehend the involvement of ECM modifications in the oncogenic transformation of sarcoid fibroblasts. Thus, the aim of this investigation was to describe the expression patterns of a set of genes that are essential for the rearrangements of the extracellular matrix, namely collagen genes, and elucidate possible mechanisms underlying the observed disruptions. To this end, we applied the RT-qPCR method on BPV-negative skin samples and sarcoid samples (<em>n</em> = 6 and 7; respectively) to perform relative quantification of the expression level of eight genes belonging to the collagen family and carried out an integrative analysis of the obtained data with previously characterized epigenetic signatures. The results showed aberrations in the level of chosen collagen genes in the sarcoids compared to the control, manifesting in their elevated levels in the tumor samples (<em>p</em>-value≤0.05). The upregulation of <em>Col1A2, Col11A1, Col6A3, Col5A2, Col4A1, Col6A6, Col5A1, Col6A2</em> genes was detected in sarcoid samples. The identified changes were statistically significant (<em>p</em>-value≤0.05) and ranged from 1.43 (Col6A2) to 1.88 (Col6A3). Further investigation into the potential involvement of epigenetic mechanisms in the regulation of collagen gene levels in sarcoids revealed compelling evidence of DNA methylation and microRNAs playing significant roles. The findings suggest a complex interplay between gene expression, epigenetic regulation, and the dysregulation of the ECM in sarcoid pathogenesis.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105656"},"PeriodicalIF":2.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attitudes towards cattle handling among Western Canadian cow-calf handlers: The role of handling training and perspectives on beef cattle welfare 加拿大西部小牛处理者对牛处理的态度：处理训练的作用和对肉牛福利的看法

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-18 DOI: 10.1016/j.rvsc.2025.105654

Nathanael H. Lutevele , Karin Orsel , Meagan T.M. King , Ed A. Pajor , Maria C. Ceballos

{"title":"Attitudes towards cattle handling among Western Canadian cow-calf handlers: The role of handling training and perspectives on beef cattle welfare","authors":"Nathanael H. Lutevele , Karin Orsel , Meagan T.M. King , Ed A. Pajor , Maria C. Ceballos","doi":"10.1016/j.rvsc.2025.105654","DOIUrl":"10.1016/j.rvsc.2025.105654","url":null,"abstract":"<div><div>Western Canadian cow-calf handlers (<em>n</em> = 118) were surveyed to determine: (1) attitudes towards beef cattle and handling; (2) influence of handling training on handling attitudes; (3) cattle welfare assessment strategies and improvement prioritization areas, and (4) involvement in and perspectives about handling training. Respondents agreed with statements related to positive attitudes, including the importance of proper handling practices, knowledge, and patience for effective cattle handling. They also agreed with statements favouring cattle cognitive abilities and the importance of good handling facilities, while disagreed with statements indicating little knowledge is required to handle cattle and the use of prods, hitting cattle, and yelling. Key welfare assessment indicators that participants reported using were general health and body condition scores, and they prioritized improving nutrition, herd health programs, and pain management. Economic returns and a sense of responsibility were major motivations for improving cattle welfare. Most respondents had attended cattle handling training, including in-person with hands-on demonstrations (33.7 %), in-person without hands-on demonstrations (10.9 %), hybrid formats combining online modules with hands-on demonstrations (19.6 %), and fully online training (35.9 %). Safety and desire to learn were major motivations for participating in handling training. Respondents acknowledged the benefits of handling training on improving handling skills and strongly agreed that handling training should focus on teaching patience strategies. Although training influenced attitudes, impacts of training modalities were inconsistent. The effectiveness of various training approaches may vary depending on the individual or context, indicating a need for further research to determine which methods yield the most positive outcomes for both handlers and cattle.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105654"},"PeriodicalIF":2.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-occurrence of leptospirosis and Opisthorchis viverrini infection in cats and their risk factors 猫钩端螺旋体病与猪腹弧菌共发感染及其危险因素分析

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-17 DOI: 10.1016/j.rvsc.2025.105657

Morsid Andityas , Pornphutthachat Sota , Peerapol Sukon , Prasarn Tangkawattana , Banchob Sripa , Ruttayaporn Ngasaman , Sirikachorn Tangkawattana

{"title":"Co-occurrence of leptospirosis and Opisthorchis viverrini infection in cats and their risk factors","authors":"Morsid Andityas , Pornphutthachat Sota , Peerapol Sukon , Prasarn Tangkawattana , Banchob Sripa , Ruttayaporn Ngasaman , Sirikachorn Tangkawattana","doi":"10.1016/j.rvsc.2025.105657","DOIUrl":"10.1016/j.rvsc.2025.105657","url":null,"abstract":"<div><div>Leptospirosis is a waterborne disease with a worldwide distribution. In Northeast Thailand, its prevalence overlaps with the endemicity of <em>Opisthorchis viverrini,</em> (OV). This study aimed to investigate the prevalence and risk factors of leptospirosis in cats and its association with opisthorchiasis in endemic area of Thailand. 115 cats were sampled for blood and feces. We tested <em>Leptospira</em> antibodies in blood using the Lepto-latex test. For fecal analysis, we applied the formalin-ether concentration technique (FECT) for OV and identified <em>Leptospira</em> spp. through PCR targeting the 16S rRNA and LipL32. Non-spatial analyses included Pearson's Chi-square, Fisher's exact test, and logistic regression, while spatial analyses involved Inverse Distance Weighted (IDW), spatial autocorrelation, and Geographically Weighted Logistic Regression (GWLR). Serological analysis revealed 15.65 % of cats positive for <em>Leptospira</em> spp., whereas 44.35 % of fecal samples positive for OV. PCR analysis identified 1 <em>L. interrogans</em> with the 16S rRNA, while the LipL32 identified 2 samples related to L. <em>interrogans</em> and 1 to L. <em>borgpetersenii</em>. The spatial autocorrelation indicated clustering of leptospirosis cases. Logistic regression revealed 2 significant risk factors; OV infection (OR 3.44, 95 % CI 1.10–10.73; p 0.03) and proximity to flooded areas (OR 3.47, 95 % CI 1.12–10.74; p 0.03). Furthermore, GWLR showed median coefficients of 0.59 (−1.62–5.15) for OV infection and 2.15 (1.30–6.47) for proximity to flooded areas. This study demonstrates a significant association between cat leptospirosis with OV infection and proximity to flooded areas. These highlight the importance of monitoring environmental and biological risk factors to mitigate leptospirosis in endemic regions.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105657"},"PeriodicalIF":2.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of mechanical transmission of Anaplasma marginale by Stomoxys calcitrans 钙化Stomoxys calcitrans对边缘无原体机械传播的评价

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-16 DOI: 10.1016/j.rvsc.2025.105655

Luciana Maffini Heller , Dina María Beltrán Zapa , Igor Maciel Lopes de Morais , Vanessa Ferreira Salvador , Luccas Lourenzzo Lima Lins Leal , Luiz Fellipe Monteiro Couto , Lídia Mendes de Aquino , Lucianne Cardoso Neves , Bianca Barbara Fonseca da Silva , Lorena Lopes Ferreira , Antonio Thadeu Medeiros de Barros , Paulo Henrique Duarte Cançado , Felipe da Silva Krawczak , Caio Marcio de Oliveira Monteiro , Welber Daniel Zanetti Lopes

{"title":"Evaluation of mechanical transmission of Anaplasma marginale by Stomoxys calcitrans","authors":"Luciana Maffini Heller , Dina María Beltrán Zapa , Igor Maciel Lopes de Morais , Vanessa Ferreira Salvador , Luccas Lourenzzo Lima Lins Leal , Luiz Fellipe Monteiro Couto , Lídia Mendes de Aquino , Lucianne Cardoso Neves , Bianca Barbara Fonseca da Silva , Lorena Lopes Ferreira , Antonio Thadeu Medeiros de Barros , Paulo Henrique Duarte Cançado , Felipe da Silva Krawczak , Caio Marcio de Oliveira Monteiro , Welber Daniel Zanetti Lopes","doi":"10.1016/j.rvsc.2025.105655","DOIUrl":"10.1016/j.rvsc.2025.105655","url":null,"abstract":"<div><div>The objective of this study was to investigate the mechanical transmission of <em>Anaplasma marginale</em> to cattle by <em>Stomoxys calcitrans</em>. The <em>S. calcitrans</em> were obtained in field condition and their offspring (first generation) were used in this study originating in the laboratory. In method 1 (flies released freely in cattle pens), from D0 to D + 10, approximately 3300 flies fasted for 24 h were released daily per pen with infected and healthy calves. In the second method (feeding chambers for artificial interruption of blood meals), for each negative calf, three chambers were used, each containing four flies fasted for 24 h, totaling 12 flies per calf. Blood smears, PCR and ELISA were performed to detect <em>A. marginale</em> in the animals. Furthermore, the animals' head tossing and hoof stomping and the number of flies near the pens' inner walls were recorded. In the presence of the flies, the calves exhibited defensive movements compared to those without flies. None of the negative calves demonstrated the presence of <em>A. marginale</em> (blood smear, PCR, and ELISA). In conclusion, although <em>A. marginale</em> was found on the abdomen of <em>S. calcitrans</em>, this fly failed to transmit this rickettsia to cattle in either transmission method employed.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105655"},"PeriodicalIF":2.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytauxzoon europaeus, Babesia vulpes, and Hepatozoon felis circulating simultaneously in a European wildcat (Felis silvestris) population in northern Spain 在西班牙北部的欧洲野猫（felis silvestris）种群中，欧洲巴贝斯虫、秃鹫巴贝斯虫和猫科动物肝虫同时传播

IF 2.2 3区农林科学

Research in veterinary science Pub Date : 2025-04-15 DOI: 10.1016/j.rvsc.2025.105653

Clara Muñoz-Hernández , Diego Villanúa , Rocío Checa , Marta Sánchez-Sánchez , Efrén Estévez-Sánchez , Alberto Moraga-Fernández , Fermín Urra , Guadalupe Miró , Isabel G. Fernández de Mera , Javier Millán

{"title":"Cytauxzoon europaeus, Babesia vulpes, and Hepatozoon felis circulating simultaneously in a European wildcat (Felis silvestris) population in northern Spain","authors":"Clara Muñoz-Hernández , Diego Villanúa , Rocío Checa , Marta Sánchez-Sánchez , Efrén Estévez-Sánchez , Alberto Moraga-Fernández , Fermín Urra , Guadalupe Miró , Isabel G. Fernández de Mera , Javier Millán","doi":"10.1016/j.rvsc.2025.105653","DOIUrl":"10.1016/j.rvsc.2025.105653","url":null,"abstract":"<div><div>The Iberian population of the European wildcat (<em>Felis silvestris</em>) is undergoing a decline and is highly fragmented. This study aimed to determine the occurrence and identity of tick-borne parasites (piroplasms and <em>Hepatozoon</em> spp.) in Navarre, one of the strongholds for wildcats in Spain. DNA was extracted from spleen samples of 63 road-killed wildcats and analyzed using a suite of molecular protocols targeting the 18S rRNA and CytB gene fragments. Eighty percent of the wildcats tested positive for at least one parasite. <em>Cytauxzoon</em> spp. was detected in 65 % of the individuals. Molecular characterization and phylogenetic analysis confirmed the presence of <em>Cytauxzoon europaeus</em> in nine samples, representing the first report of this species in the Iberian Peninsula. <em>Hepatozoon</em> spp. was found in 46 % of the wildcats, and molecular characterization of four cases identified <em>Hepatozoon felis</em>. <em>Babesia vulpes</em> was detected in a single individual. Coinfection with <em>C</em><em>ytauxzoon</em> sp. and <em>H</em><em>epatozoon</em> sp. was observed in 32 % of the wildcats, while one individual was coinfected with <em>C. europaeus</em> and <em>B. vulpes</em>. Occurrence of <em>H. felis</em> was higher in adult wildcats. No spatial structure or correlation with body condition was observed for any of the parasites. Given the widespread presence of <em>Cytauxzoon</em> and other parasites in this and other wildcat populations, their impact on wildcat health, if any, is likely to be minimal. However, wildcats appear to serve as important maintenance hosts for these parasites.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"190 ","pages":"Article 105653"},"PeriodicalIF":2.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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