犬乳腺肿瘤中VISTA表达的免疫组织化学分析：免疫治疗靶向的初步研究

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-09 DOI:10.1016/j.rvsc.2025.105807

Muhammed Ali Yamac , Leyla Elif Ozgü Ayozger , Volkan Ipek

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引用次数: 0

摘要

VISTA （V-domain Ig-containing suppressor of T cell activation）是一种调节T细胞活性并参与免疫耐受的免疫检查点蛋白。虽然它在人类肿瘤中的作用已被阐明，但其在犬乳腺肿瘤（cmt）中的意义尚不清楚。本研究的重点是研究VISTA在CMTs中的表达及其与组织学分级和分子亚型的关系。本文对26例诊断为恶性肿瘤的犬乳腺肿瘤进行了福尔马林固定、石蜡包埋的研究。采用苏木精和伊红染色切片进行组织病理学分类和分级。对雌激素受体（ER）、孕激素受体（PR）、HER2和VISTA进行免疫组化染色，并根据ER、PR和HER2状态定义分子亚型。在肿瘤细胞和炎症细胞中半定量评价VISTA免疫反应性。肿瘤细胞和炎性浸润细胞中可见VISTA的表达。肿瘤分级增加，肿瘤细胞的VISTA染色强度和总体评分显著增加。炎症细胞染色随分级不同而不同，但差异不显著。VISTA在分子亚型中的表达无显著差异。这是阐明VISTA在CMTs中的表达的初步研究。它在肿瘤细胞和浸润细胞内的识别，以及它与肿瘤分级的相关性，使得它作为犬乳腺癌免疫治疗和预后标志物具有重要意义。

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Immunohistochemical profiling of VISTA expression in canine mammary tumors: A pilot study for immunotherapeutic targeting

VISTA (V-domain Ig-containing suppressor of T cell activation) is an immune checkpoint protein that regulates the activity of T cells and is involved in immune tolerance. Although its role has been elucidated in human oncology, its significance in canine mammary tumors (CMTs) is unclear. This research was focused on investigating VISTA expression in CMTs and its correlation with histologic grade and molecular subtype. Archived formalin-fixed, paraffin-embedded mammary gland tumors taken from 26 canine patients diagnosed with malignant tumors were studied. Histopathologic classification and grading were performed using hematoxylin and eosin–stained sections. Immunohistochemical staining for estrogen receptor (ER), progesterone receptor (PR), HER2, and VISTA was done, and molecular subtypes were defined as based on ER, PR, and HER2 status. VISTA immunoreactivity was semiquantitatively evaluated in tumor cells and in inflammatory cells. VISTA expression in neoplastic cells and in cells of the inflammatory infiltrate was found. Increased tumor grade was found to have significantly increased VISTA staining intensity and overall score in tumor cells. Inflammatory cell staining differed according to grade, yet this difference was not significant. No significant difference in VISTA expression was detected in molecular subtypes. This is the initial research to shed light on VISTA expression in CMTs. Its identification within tumor cells and within cells of the infiltrate, and its correlation with tumor grade, places its importance as an immunotherapeutic and prognostic marker in mammary cancer in dogs.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.