Chondroprotective effects of ozone and hyaluronic acid in rat knee osteoarthritis: Comparison of intra-articular and systemic administration

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-05 DOI:10.1016/j.rvsc.2025.105798

Elif Unlu , Nihal Y. Gül Satar , Onur Onguncan , Sabire Güler , Nevzat Uslu , M. Omer Ukum

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引用次数: 0

Abstract

This study aimed to evaluate the therapeutic efficacy of intra-articular (i.a.) ozone, hyaluronic acid (HA), their combination (ozone–HA), and systemic (intraperitoneal, i.p.) ozone in a rat model of surgically induced knee osteoarthritis (OA). OA was induced by anterior cruciate ligament transection in 60 female Sprague Dawley rats randomized into six groups: i.a. control (saline), i.a. HA, i.a. ozone, i.a. ozone–HA, i.p. control, and i.p. ozone. Treatments were administered weekly for five weeks, starting on day 14 post-surgery. Therapeutic outcomes were assessed using computed tomography, histopathological scoring (Modified Mankin, cartilage degeneration, and matrix loss width), immunohistochemistry (type II collagen, MMP-13), and serum TNF-α levels. At week eight, CT imaging revealed reduced structural damage in the i.a. ozone–HA group. The i.a. ozone–HA group had the lowest Modified Mankin score, significantly lower than the i.a. control (p < 0.001) and i.p. control (p = 0.007) groups. Cartilage matrix loss was significantly reduced in treated groups, particularly in the superficial zone (p = 0.010). Cartilage degeneration scores in the Z1 region were significantly improved in the i.a. ozone (p = 0.011) and i.a. ozone–HA (p = 0.004) groups compared to i.a. control. Additionally, type II collagen expression was significantly enhanced in both i.a. ozone and ozone–HA groups (p = 0.010). These results indicate that intra-articular ozone, particularly combined with HA, offers superior chondroprotective and regenerative effects compared to HA alone or systemic ozone, highlighting its potential as a novel therapeutic approach for early-stage osteoarthritis in veterinary medicine.

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臭氧和透明质酸对大鼠膝关节骨性关节炎的软骨保护作用：关节内和全身给药的比较

本研究旨在评价关节内（i.a）臭氧、透明质酸（HA）及其组合（臭氧- HA）和全身（腹腔）臭氧对大鼠手术性膝骨关节炎（OA）模型的治疗效果。将60只雌性Sprague Dawley大鼠随机分为6组：对照组（生理盐水组）、HA组、臭氧组、HA - HA组、对照组和臭氧组。从术后第14天开始，每周进行5周的治疗。通过计算机断层扫描、组织病理学评分（改良曼金、软骨退变和基质损失宽度）、免疫组织化学（II型胶原、MMP-13）和血清TNF-α水平评估治疗结果。第8周，CT成像显示臭氧- ha组结构损伤减轻。臭氧- ha组的改良曼金评分最低，显著低于对照组(p <；0.001)和对照组（p = 0.007）。治疗组软骨基质损失明显减少，特别是在浅表区（p = 0.010）。与对照组相比，臭氧组（p = 0.011）和ha -臭氧组（p = 0.004） Z1区软骨退行性变评分显著提高。此外，臭氧组和臭氧- ha组II型胶原蛋白表达均显著增强（p = 0.010）。这些结果表明，与单独使用透明质酸或全身臭氧相比，关节内臭氧，特别是与透明质酸联合使用，具有更好的软骨保护和再生作用，突出了其作为兽医学早期骨关节炎新治疗方法的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.