巴西一家兽医医院犬、猫和人对甲氧西林耐药葡萄球菌的流行、鉴定和抗菌敏感性分析

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-05 DOI:10.1016/j.rvsc.2025.105793

Ricardo Antonio Pilegi Sfaciotte , Leandro Parussolo , Fernanda Danielle Melo , Luis Henrique Pompelli , Naiara Dognani Israel , David Germano Gonçalves Schwarz , Ubirajara Maciel da Costa , Sandra Maria Ferraz

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引用次数: 0

摘要

耐甲氧西林葡萄球菌（MRS）的存在不仅局限于人类和患病动物，也可以在健康动物和兽医医院工作的专业人员中定植。本研究的目的是调查某大学兽医院住院动物和工作人员中MRS的存在情况，并确定葡萄球菌的主要种类及其抗菌药物敏感性。在106只动物入院时采集鼻拭子，在患者出院后采集鼻拭子。从参与医院日常工作的工作人员中收集了20份鼻拭子。用多重PCR法检测MRS菌株的药敏谱和葡萄球菌种类。结果发现，48.11%的动物mrs呈阳性，其中狗38.7%，猫10.4%。从动物分离的76例MRS中，52.63%为MRSA， 40.76%为MRSP， 6.58%为MRSS。所有表型鉴定的MRS都携带mecA基因。其中MRS占90%，MRSA占66.67%，MRSP占33.33%。工作人员和动物中存在MRS，突出表明这些病原体在兽医医院内广泛传播，有助于多重耐药微生物的传播。

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Prevalence, identification and antimicrobial sensitivity profile of methicillin resistant Staphylococcus colonizing dogs, cats and humans in a veterinary hospital, Brazil

The presence of Methicillin-resistant Staphylococcus (MRS) is not limited to humans and sick animals but can also colonize healthy animals and professionals working in veterinary hospitals. The aim of this study was to investigate the presence of MRS in hospitalized animals and staff at a university veterinary hospital, as well as to identify the main species of Staphylococcus and their antimicrobial susceptibility profile. Nasal swabs were collected from 106 animals upon admission to the Veterinary Clinical Hospital and another swab after the patients were discharged from hospital. 20 nasal swabs were collected from staff involved in the routine of the Hospital. Strains identified as MRS were tested for their antimicrobial susceptibility profile and identification of Staphylococcus species by the multiplex PCR. It was found that 48.11 % of the animals were positive for MRS. Of these, 38.7 % were dogs and 10.4 % were cats. From the 76 MRS isolated from animals, 52.63 % were identified as MRSA, 40.76 % as MRSP, and 6.58 % as MRSS. All phenotypically identified MRS carried the mecA gene. As for the individuals, 90 % were colonized by MRS, with 66,67 % by MRSA and 33.33 % by MRSP. The presence of MRS in the staff and, animals highlights the widespread dissemination of these pathogens within a veterinary hospital, contributes to the spread of multidrug-resistant microorganisms.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.