Melatonin improves growth and slaughter performance of broilers by regulating satellite cell proliferation via RORα nuclear receptor

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-25 DOI:10.1016/j.rvsc.2025.105823

Shuhui Ma , Lina Dong , Aili Fan , Yang Li , Yuzhe Fan , Jiayin Liu , Liang Hong , Zonghui Zuo , Daowen Li , Gang Xu

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引用次数: 0

Abstract

To investigate the molecular mechanisms of melatonin on the growth and slaughter performance of broilers, 144 newly hatched male broilers were randomly assigned to dietary melatonin (0, 5, 20, or 80 mg/kg) for 42 days. Chicken skeletal muscle satellite cells (SMSCs) were treated with melatonin (0.5–312.5 ng/mL), and retinoid-related orphan receptor-alpha (RORα) involvement was investigated using agonist SR1078 or inverse agonist SR3335. Growth and slaughter performance, serum antioxidant capacity, meat quality, as well as molecular expression levels analyzed for skeletal muscle growth related factor were conducted. The results demonstrated dietary melatonin significantly decreased feed gain ratio, and elevated muscle percentage (P < 0.05), serum melatonin content (P < 0.05), total antioxidation capability (T-AOC) and the activity of antioxidant enzymes (P < 0.05), while reduced the levels of malondialdehyde (MDA) (P < 0.05). Administration of melatonin notably improved the water-holding capacity and myofiber area of muscles (P < 0.05). Additionally, melatonin significantly upregulated the expression of paired domain homeobox transcription factor 7 (Pax7), myoblast determination protein (MyoD), and myogenic factor 5 (Myf5) in muscles and SMSCs, downregulated Myostatin (MSTN) (P < 0.05). In the presence of melatonin, the SR1078 markedly enhanced Pax7 expression, while the SR3335 reduced the expression of Pax7, MyoD, and Myf5 (P < 0.05). In conclusion, melatonin has the potential to regulate the proliferation of chicken SMSCs through the RORα nuclear receptor, affecting the expression levels of skeletal muscle growth related factor in SMSCs, promoting the growth and slaughter performance of broilers.

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褪黑素通过rna α核受体调节卫星细胞增殖，提高肉鸡生长性能和屠宰性能

为研究褪黑素对肉仔鸡生长和屠宰性能影响的分子机制，试验选用144只刚孵化的雄性肉仔鸡，随机饲喂褪黑素（0、5、20和80 mg/kg） 42 d。用褪黑素（0.5 ~ 312.5 ng/mL）处理鸡骨骼肌卫星细胞（SMSCs），并使用激动剂SR1078或逆激动剂SR3335研究类视黄酮相关孤儿受体α (rora)的参与情况。对生长性能、屠宰性能、血清抗氧化能力、肉品质及骨骼肌生长相关因子的分子表达水平进行分析。结果表明，饲粮中褪黑素显著降低了饲料增重比，提高了肌肉率(P <；0.05)，血清褪黑素含量(P <；0.05)、总抗氧化能力（T-AOC）和抗氧化酶活性(P <；0.05)，同时降低丙二醛（MDA）水平(P <；0.05)。褪黑素显著改善了肌肉的持水能力和肌纤维面积(P <；0.05)。此外，褪黑素显著上调成对结构域同源盒转录因子7 （Pax7）、成肌细胞决定蛋白（MyoD）和肌生成因子5 （Myf5）在肌肉和SMSCs中的表达，下调肌肉生长抑制素（MSTN） (P <；0.05)。在褪黑激素存在下，SR1078显著增强了Pax7的表达，而SR3335则降低了Pax7、MyoD和Myf5的表达(P <；0.05)。综上所述，褪黑素可能通过RORα核受体调控鸡SMSCs的增殖，影响SMSCs中骨骼肌生长相关因子的表达水平，促进肉鸡生长和屠宰性能。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.