Isolation and characterization of Bovine Gammaherpesvirus-4 (BoHV-4) in diarrheic sheep

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-22 DOI:10.1016/j.rvsc.2025.105819

Kadir Yeşilbağ , Mevlüt Yaşar , Zafer Ata

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引用次数: 0

Abstract

While commonly detected in cattle, the presence of Bovine gammaherpesvirus 4 (BoHV-4) in small ruminants remains insufficiently examined. This study reports the isolation, identification, and genetic characterization of BoHV-4 from a sheep presenting hemorrhagic diarrhea. A fecal sample collected from an infected adult sheep was submitted to a diagnostic study using a real-time PCR panel for various bacteria, parasites, and viruses, yielding negative results for all the pathogens in the panel. Virus isolation was performed on MDBK, BHK-21, SFT-R cell lines, and primary lamb kidney cell culture (PLK), and cytopathogenic effects were observed in all the cell lines except for PLK. The virus was then identified through chloroform and halogen assays. The presence of BoHV-4 was validated by nested PCR targeting the thymidine kinase (TK) gene, and phylogenetic analysis classified the isolate as Genotype II, showing close genetic similarity with Argentinean strains. Serum neutralization assay demonstrated low cross-reactivity between BoHV-1 polyclonal serum and BoHV-4 isolate. This study reports the first detection of BoHV-4 genotype II in sheep and suggests its potential association with gastrointestinal disease. Further studies are needed to investigate the pathogenic role of BoHV-4 in small ruminants and its impact on various organ systems.

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牛γ -疱疹病毒-4 （BoHV-4）在腹泻羊中的分离与鉴定

牛γ -疱疹病毒4 （BoHV-4）通常在牛中检测到，但在小反刍动物中的存在仍未得到充分检查。本研究报道了从出现出血性腹泻的绵羊中分离、鉴定和BoHV-4的遗传特征。从一只受感染的成年羊身上收集粪便样本，使用实时荧光定量PCR检测各种细菌、寄生虫和病毒，进行诊断研究，检测结果显示所有病原体均为阴性。对MDBK、BHK-21、SFT-R细胞系和原代羊肾细胞培养（PLK）进行病毒分离，除PLK外，其余细胞系均有细胞致病性。然后通过氯仿和卤素测定鉴定出病毒。针对胸苷激酶（TK）基因的巢式PCR验证了BoHV-4的存在，系统发育分析将分离物归类为基因型II，与阿根廷菌株具有密切的遗传相似性。血清中和试验表明，BoHV-1多克隆血清与BoHV-4分离物的交叉反应性较低。本研究报道了首次在绵羊中检测到BoHV-4基因型II，并提示其与胃肠道疾病的潜在关联。BoHV-4在小反刍动物中的致病作用及其对各器官系统的影响有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.