Research in veterinary science最新文献

{"title":"Evaluation of serum and fecal parameters in cats with low-grade intestinal T-cell lymphoma (LGITCL)","authors":"Alessandra Gavazza ,&nbsp;Sara Mangiaterra ,&nbsp;Livio Galosi ,&nbsp;Alessia Dottori ,&nbsp;Lucia Biagini ,&nbsp;Graziano Pengo ,&nbsp;Jan Suchodolski ,&nbsp;Matteo Cerquetella ,&nbsp;Giacomo Rossi","doi":"10.1016/j.rvsc.2024.105521","DOIUrl":"10.1016/j.rvsc.2024.105521","url":null,"abstract":"<div><div>Lymphoma is the most common neoplasia in the intestine of cats. According to ACVIM consensus statement, low-grade intestinal T-cell lymphoma (LGITCL) represents a monomorphic infiltration of the lamina propria or epithelium or both of cats with small, mature, neoplastic (clonal) T lymphocytes. Despite the importance as contributing factors of inheritance and environment in the pathogenesis of LGITCL, the chronic inflammatory status plays a fundamental role. The aim of the present study was to investigate possible diagnostic and prognostic parameters in LGITCL. Selected fecal bacteria and serum biomarkers (serum amyloid A [SAA]; citrulline; total protein; amylase; lipase; DGGR lipase; cholesterol; lipopolysaccharides [LPS], and zonulin) were evaluated in 12 cats (lymphoma group – LG) with histologically diagnosed low-grade intestinal T- cell lymphoma (LGITCL) and then compared with a control group consisting of 12 clinically healthy cats (CG). The evaluation of fecal bacterial population showed the significant decrease of <em>Faecalibacterium</em> spp. (<em>P</em> = 0,045) and <em>Clostridium hiranonis</em> (<em>P</em> = 0,0433) and a significant increase in <em>E. coli</em> (P = 0,045), <em>Streptococcus</em> spp. (P = 0,0003) and <em>Turicibacter</em> spp. (<em>P</em> = 0,0056) in the lymphoma group. Serology showed a significant decrease of total proteins (<em>P</em> = 0,092), amylase (P = 0,092) and cholesterol (P = 0,0112) in LG group compared to controls. The present results allowed the authors to state that in cats LGITCL is possibly associated to a change in the gastrointestinal environment and to a condition of protein losing enteropathy. Further studies with a larger cohort of patients are needed to confirm the present results and to point out other possible changes.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105521"},"PeriodicalIF":2.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis","authors":"Ximena Ferrara Muñiz ,&nbsp;Wanderson Marques da Silva ,&nbsp;Sergio Gabriel Garbaccio ,&nbsp;Carlos Javier Garro ,&nbsp;Romina Ayelén Sammarruco ,&nbsp;Micaela Encinas ,&nbsp;Hugo Adrián Carignano ,&nbsp;María Verónica Bianco ,&nbsp;Martín José Zumárraga ,&nbsp;Ángel Adrián Cataldi ,&nbsp;María Emilia Eirin","doi":"10.1016/j.rvsc.2024.105513","DOIUrl":"10.1016/j.rvsc.2024.105513","url":null,"abstract":"<div><div>Bovine tuberculosis (bTB), a global zoonotic disease, causes negative effects on human and animal health. PhoP protein is a key regulator of pathogenic phenotypes in members of the <em>Mycobacterium tuberculosis complex</em>, which includes the causative agent of bTB. Despite extensive research on this protein focused in deciphering its regulatory role, little was explored about it as a diagnostic antigen. In humans, a novel role of anti-PhoP antibodies as a possible marker for the diagnosis of TB was demonstrated. However, this issue was not addressed in bovines. In this study, antigenic properties of the PhoP protein were evaluated in naturally <em>Mycobacterium bovis</em> (<em>M. bovis</em>) infected bovines. A high homology of PhoP (≥ 75 %) was observed in environmental mycobacterial species and other genera such as <em>Salmonella</em> and <em>Pasteurella</em>. Using the IFN-gamma release assay (IGRA), we detected cell-mediated immune response against PhoP in cattle from infected herds (25 %; IC 95 % 3.2–65.1), although it was significantly lower than that evoked by the reference antigens, ESAT-6/CFP-10/Rv3615c (75 %; IC95 % 34.9–96.8), and the purified protein derivative (87.5 %; IC 95 % 47.4–99.7) (<em>p</em> &lt; 0.05)). Animals from a bTB free area showed no response against PhoP when analyzed by IGRA. Although, the humoral response detected 62.5 % (CI95% 24.5–91.5) of naturally infected animals, there was 100 % cross-reactivity among TB-free cattle. These results suggest that the PhoP protein is not a promising candidate for bTB diagnosis, due to it had relatively low levels of test sensitivity in the IGRA test, and very low specificity in a humoral antibody western blot assay.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105513"},"PeriodicalIF":2.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The presence of sexed sperm in bovine oviduct epithelial cells alters the protein profile related to stress and immune response","authors":"Daiane Gonzaga Ribeiro ,&nbsp;José de Oliveira Carvalho ,&nbsp;Roberto Sartori ,&nbsp;Pedro Leopoldo Jerônimo Monteiro ,&nbsp;Wagner Fontes ,&nbsp;Mariana de Souza Castro ,&nbsp;Marcelo Valle de Sousa ,&nbsp;Margot Alves Nunes Dode ,&nbsp;Angela Mehta","doi":"10.1016/j.rvsc.2024.105522","DOIUrl":"10.1016/j.rvsc.2024.105522","url":null,"abstract":"<div><div>Although sperm sexing technology has progressed considerably in the last decade, there are still challenges to fully understand the reason for the low fertility of sexed sperm. Thus, we aimed to evaluate the effect of sexed and non-sexed sperm on the proteome of bovine oviduct epithelial cells (BOECs). Semen from six Nellore bulls was used and one ejaculate from each bull was collected and separated into three fractions: non-sexed, sexed for X-sperm and sexed for Y-sperm. Previously synchronized females were artificially inseminated with either a pool of non-sexed sperm from 6 sires (NS), or a pool of sexed X and Y sperm from 6 sires (XY) or saline solution (Control). After insemination, animals were slaughtered and oviducts were collected to obtain BOECs samples, which were used for proteomic analysis. The results revealed that the oviductal response on isthmus region to the presence of sperm is different when sexed and non-sexed sperm are used. Sexed sperm seemed to induced a more intense and imbalanced response to several processes, such as oxidative and heat stress, immune response and movement of the oviduct muscle.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105522"},"PeriodicalIF":2.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donkey endometrium: Characterization of resident immune cells","authors":"Ariana Radar-Chafirovitch ,&nbsp;Miguel Quaresma ,&nbsp;Ivan Yánez-Ortiz ,&nbsp;Belén Leiva ,&nbsp;Graça Ferreira-Dias ,&nbsp;Rita Payan-Carreira ,&nbsp;Jordi Miro ,&nbsp;Maria dos Anjos Pires","doi":"10.1016/j.rvsc.2024.105516","DOIUrl":"10.1016/j.rvsc.2024.105516","url":null,"abstract":"<div><div>The <em>Burro de Miranda</em> is an endangered donkey breed. The dynamics of the immune system of the donkey's reproductive tract are essential to manage the fertility of these animals for the improvement and survival of the species. In mares, high numbers of immune cells infiltrating the endometrium promote endometrosis, which is still to be confirmed in jennies. Uterine biopsies of 32 jennies were evaluated based on the Kenney and Doig grading system used in mares. Hematoxylin and eosin stain was used to assess the infiltration and distribution of neutrophils and eosinophils. Macrophage and B and T lymphocytes endometrial distribution was conducted through immunohistochemistry.</div><div>T lymphocytes were the most predominant cells in jenny endometrium, macrophages being the second. T lymphocytes were also found in the superficial and glandular epithelium. Eosinophils, neutrophils and B lymphocytes were the least common cells. No differences were found in the inflammatory infiltrate compared to the different endometrosis grades (IIA, IIB and III).</div><div>This study mapped the immune cells in jenny's endometrium, providing core valuable information for additional immunological and reproductive studies in this species. It also highlighted significant differences in endometrial immune cell distribution between the jenny and the mare during estrus and diestrus, supporting the need to develop of a more suitable scoring system than the current Kenney and Doig categorization to assess the morphology and clinical feature of jenny's endometrium.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105516"},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental exposure and cancer occurrence in dogs: a critical appraisal of evidence.","authors":"Kei Owada ,&nbsp;Emma Nicholls ,&nbsp;Ricardo J. Soares Magalhães ,&nbsp;Chiara Palmieri","doi":"10.1016/j.rvsc.2024.105517","DOIUrl":"10.1016/j.rvsc.2024.105517","url":null,"abstract":"<div><div>Cancer is a significant contributor to morbidity and mortality in humans and is also the leading cause of death in dogs. It is estimated that up to 20 % of human cancers can be explained by environmental exposures to carcinogens. It has been hypothesized that companion animals such as dogs could not only be a model for the complex pathogenic processes of human cancers, but also act as sentinels for environmental carcinogens due to their relative shorter longevity, spontaneous occurrence of tumours, and intimate relationship with our everyday environments. This review aimed to provide the epidemiological evidence on the role of the environment in the development and progression of tumours in the canine species.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105517"},"PeriodicalIF":2.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamical modeling of Salmonellosis in humans and dairy cattle with temperature and pH effects","authors":"Herman Trazias ,&nbsp;Maranya Mayengo ,&nbsp;Jacob Irunde ,&nbsp;Moatlhodi Kgosimore","doi":"10.1016/j.rvsc.2024.105514","DOIUrl":"10.1016/j.rvsc.2024.105514","url":null,"abstract":"<div><div>Approximately 20 million cases and 0.15 million human fatalities worldwide each year are caused by Salmonellosis. A mechanistic compartmental model based on ordinary differential equations is proposed to evaluate the effects of temperature and pH on the transmission dynamics of Salmonellosis. The transmission potential of the disease in areas with temperature and pH stresses is examined. The next-generation matrix method is applied to compute the temperature-pH-dependent reproduction number <span><math><msub><mi>ℛ</mi><mi>PT</mi></msub></math></span>. The dynamical regimes of the system are examined using Lyapunov stability theory and backward bifurcation analysis. The uncertainty and global sensitivity analysis are examined using the Latin Hypercube Sampling (LHS) and Partial Rank Correlation Coefficient (PRCC) methods. The numerical simulations of the proposed model under favorable and unfavorable temperatures are performed with a <span><math><mn>95</mn><mo>%</mo></math></span> confidence interval (CI) for the reliability assessment of the model parameters. The analysis shows that the ingestion rates of <em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Typhimurium bacteria in humans and dairy cattle, human-to-human transmission rate, cattle-to-cattle transmission rate, human shedding rate, dairy cattle shedding rate, and the rate of producing contaminated dairy products are directly proportional to the number of infected humans and infected dairy cattle. The temperature ranges of <span><math><msup><mn>10</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi><mo>−</mo><msup><mn>20</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi></math></span> and <span><math><msup><mn>30</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi><mo>−</mo><msup><mn>40</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi></math></span> and pHs greater than 3.8 have a significant effect on the dynamics of Salmonellosis. In order to eliminate Salmonellosis, the study recommends treating natural water bodies using the recommended chemical disinfectants during summer seasons and in areas with temperature ranges of <span><math><msup><mn>10</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi><mo>−</mo><msup><mn>20</mn><mn>0</mn></msup><mspace></mspace><mi>C</mi></math></span>, cooking food at the hottest temperatures, and storing food at the lowest temperatures for all pHs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105514"},"PeriodicalIF":2.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of visfatin in peripheral immune organs and intestines of weaned piglets under lipopolysaccharide induced immune stress","authors":"Huizhen Li ,&nbsp;Xiaoyu Niu ,&nbsp;Fenliang Xu ,&nbsp;Abdur Rahman Ansari ,&nbsp;Weihua Zou ,&nbsp;Keli Yang ,&nbsp;Xinxin Pang ,&nbsp;Hui Song","doi":"10.1016/j.rvsc.2024.105499","DOIUrl":"10.1016/j.rvsc.2024.105499","url":null,"abstract":"<div><div>To investigate the regulatory mechanisms and pathways of visfatin under immune stress injury in weaned piglets, we established a lipopolysaccharide-induced immune stress model in weaned piglets to study how visfatin affects peripheral immune organs and intestinal function. The results revealed that visfatin improved the inflammatory response in immune-stressed weaned piglets by reducing the levels of pro-inflammatory cytokines interleukin-1β, interleukin-6 and monocyte chemoattractant protein-1, as well as decreasing the neutrophil/lymphocyte ratio. Visfatin ameliorated oxidative stress in piglets by promoting the expression of superoxide dismutase and glutathione peroxidase. It also enhanced cell proliferation in peripheral immune organs (spleen and mesenteric lymph nodes) and suppressed cell apoptosis in these organs through the death receptor apoptosis pathway, thereby improving the immune function of weaned piglets under immune stress. Moreover, it alleviated intestinal villi damage, increased the abundance of beneficial bacteria, and elevated the levels of short-chain fatty acids, thus preserving the intestinal barrier's integrity and the balance of intestinal microbiota. Hence, these data indicate that visfatin can ameliorate immune stress injury in weaned piglets by exerting anti-inflammatory and antioxidant effects, enhancing immune organ and intestinal function.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105499"},"PeriodicalIF":2.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative pharmacokinetic evaluation of metronidazole in sheep and goats","authors":"Charbel Fadel ,&nbsp;Beata Łebkowska-Wieruszewska ,&nbsp;Firas Serih ,&nbsp;Andrzej Lisowski ,&nbsp;Amnart Poapolathep ,&nbsp;Mario Giorgi","doi":"10.1016/j.rvsc.2024.105507","DOIUrl":"10.1016/j.rvsc.2024.105507","url":null,"abstract":"<div><div>Metronidazole (MTZ) is a 5-nitroimidazole compound recognized for its dual anti-bacterial and anti-protozoal properties. Non-approved in many countries for farm medicine, due to a lack of data in the literature, the study aimed to determine its pharmacokinetics (PK) in sheep and goats following intravenous (IV) and subcutaneous (SC) administrations. Sheep (<em>n</em> = 5) and goats (n = 5) followed identical study protocols, which involved a two-phase, single-dose (2 mg/kg), cross-over study design with a one-week washout period between treatments. Blood samples were collected over 24 h for analysis. High-performance liquid chromatography (HPLC) was employed for plasma drug quantification. The analytical method exhibited excellent linearity, recovery, and precision. MTZ exhibited relatively rapid elimination in both animal species, notably faster in goats. Goats have shown a significantly higher clearance rate and consequently lower AUC values. This underscores the importance of considering species-specific differences in drug metabolism and clearance when prescribing medications for small ruminants. Recognizing the pivotal role of AUC/MIC in predicting MTZ efficacy, a dosage adjustment between these two animal species may be necessary to ensure optimal therapeutic outcomes. The bioavailability was relatively high in both animal species, suggesting the potential of SC administration as a practical approach in small ruminants, akin to recent trends in human medicine regarding MTZ administration. Additional research is needed to thoroughly assess the <em>in vivo</em> effectiveness of MTZ in diseased sheep and goats, including PK studies after multiple doses, to establish an appropriate dosing regimen for these animals.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105507"},"PeriodicalIF":2.2,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the in vitro effects of cannabidiol, cannabidiolic acid, and the terpene β-caryophyllene on lymphocytes harvested from atopic and healthy dogs: A preliminary study","authors":"Twisha Jani ,&nbsp;Domenico Santoro ,&nbsp;Justin Shmalberg","doi":"10.1016/j.rvsc.2024.105483","DOIUrl":"10.1016/j.rvsc.2024.105483","url":null,"abstract":"<div><div>Cannabidiol (CBD) has been shown to have anti-inflammatory and antipruritic properties without the significant psychoactive effects. This study aims to evaluate the cytotoxic effects of, and the production of cytokines after exposure to CBD, cannabidiolic acid (CBDA), and <em>β-</em>caryophyllene (BCP<em>)</em>, alone and in combination, by peripheral blood mononuclear cells (PBMC) from healthy and atopic dogs.</div><div>Six healthy and five atopic, privately-owned dogs were enrolled. Peripheral blood mononuclear cells were harvested and incubated for 24 h with different concentrations of CBD, CBDA, and BCP alone or in combination. Cell viability and inflammatory cytokines were assessed. There was no difference in cell viability between baseline and tested concentrations of CBD, CBDA, or BCP in either healthy or in atopic PBMC. There was no effect of CBD, CBDA and BCP on the secretion of cytokines compared to baseline in healthy or atopic PMBC. The only exception was interleukin (IL)-10, increased in healthy PMBC exposed to CBD 100 ng/mL (<em>p</em> <em>=</em> <em>0.031)</em> or CBDA 600 ng/mL (<em>p</em> <em>=</em> <em>0.017)</em>. Tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP-1), IL-2, and IL-18 were higher in atopic PBMC exposed to combinations of CBD, CBDA, and BCP compared to healthy post-exposure PBMC. This is the first study that tested the effect of CBD, CBDA, and BCP at different concentrations on atopic and healthy canine PBMC. The results of this study show that CBD, CBDA and BCP, at the tested concentrations, are safe for canine PBMC. However, CBD, CBDA and BCP did not show any direct anti-inflammatory effect under these experimental conditions. Further research is needed to confirm these results in a larger canine population.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105483"},"PeriodicalIF":2.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of GPE− strain live attenuated vaccine and CP7_E2alf strain recombinant live vaccine (marker vaccine) against Japanese epidemic classical swine fever virus isolated in 2019 and DIVA discrimination ability of the marker vaccine","authors":"Maiko Yamashita ,&nbsp;Shoko Iwamoto ,&nbsp;Mariko Ochiai ,&nbsp;Kasumi Sudo ,&nbsp;Takao Nagasaka ,&nbsp;Akito Saito ,&nbsp;Takashi Kozasa ,&nbsp;Tsutomu Omatsu ,&nbsp;Tetsuya Mizutani ,&nbsp;Kinya Yamamoto","doi":"10.1016/j.rvsc.2024.105484","DOIUrl":"10.1016/j.rvsc.2024.105484","url":null,"abstract":"<div><div>Classical swine fever (CSF) re-emerged in Japan in 2018, with the epidemic virus identified as genotype 2.1, which is moderately virulent and more difficult to detect and control than the highly virulent strain. Domestic pigs were administered with GPE⁻ strain live attenuated vaccine (GPE⁻ vaccine) for outbreak management. CP7_E2alf strain recombinant live vaccine (marker vaccine), approved for differentiation of infected from vaccinated animals (DIVA), was considered optional for obtaining CSF-free country certification issued by the World Organization for Animal Health. This study aimed to assess the efficacy of both vaccines in pigs through experimental challenge tests and evaluate the DIVA ability of the marker vaccine using two enzyme-linked immunosorbent assay (ELISA) antibody detection kits. Results showed that both GPE⁻ and marker vaccines were effective against the Japanese epidemic strain; however, the ability of the ELISA antibody detection kits to discriminate the marker vaccine was limited. Therefore, to achieve CSF-free certification using vaccines with DIVA functionality, alternative detection methods and enhancement of the sensitivity and specificity of ELISA kits are needed.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"182 ","pages":"Article 105484"},"PeriodicalIF":2.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}