Research in veterinary science最新文献

{"title":"Biphasic mammary tumor in a cat: Case report","authors":"Adina-Mihaela Pîrvu ,&nbsp;Elvira Gagniuc ,&nbsp;Cătălin Micșa ,&nbsp;Andrea Cappelleri ,&nbsp;Elena Brigandì ,&nbsp;Manuella Militaru","doi":"10.1016/j.rvsc.2025.105857","DOIUrl":"10.1016/j.rvsc.2025.105857","url":null,"abstract":"<div><div>Feline mammary tumors (FMT) are the third most common neoplasm in older domestic cats. Among these, biphasic tumors (BT) are particularly rare. BT feature two distinct neoplastic cell populations, a carcinomatous component and a non-epithelial one. This paper describes the histological and immunohistochemical features of a biphasic mammary tumor in a 7-year-old Domestic Shorthair female cat. Histopathology identified two malignant cell populations: a tubular epithelial proliferation and a highly cellular and pleomorphic mesenchymal population. Immunohistochemistry (IHC) for pan-cytokeratin, cytokeratin 18, cytokeratin 14, and cytokeratins 5/6 labeled the epithelial component of the tumor. The mesenchymal population was diffusely positive for vimentin and α-SMA, occasionally positive for calponin, and negative for p63. Ki-67 index was higher in the epithelial cells (88.8 %) compared to the interstitial cells (22.9 %). Based on histological and immunohistochemical findings, a diagnosis of carcinosarcoma was made. This study highlights the importance of including mammary BT in the differentials of feline mammary neoplasms, despite their rarity.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105857"},"PeriodicalIF":1.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Motivations and limitations to improve dairy heifer replacement programs in the west of Buenos Aires province, Argentina","authors":"Federico Demateis Llera ,&nbsp;Javier Sanchez ,&nbsp;Alejandro Larriestra ,&nbsp;Claudina Vissio","doi":"10.1016/j.rvsc.2025.105847","DOIUrl":"10.1016/j.rvsc.2025.105847","url":null,"abstract":"<div><div>Dairy heifer replacement programs (RPs) are crucial for herd health and productivity. Understanding the factors that motivate or limit their implementation could go a long way toward promoting these programs more effectively. This study aimed to quantify the relative importance of such factors among dairy farmers and advisors in the west of Buenos Aires (Argentina). For this, a choice experiment was designed using conjoint analysis (CA). The factors included therein were chosen based on the literature and expert discussions. The participants (48 farmers and 41 advisors) were asked to rank nine motivating and nine limiting profiles, i.e. combinations of factors and their corresponding levels, from 1 (most motivating/limiting) to 9 (least motivating/limiting). The part-worth utility value and relative importance of each factor and level was then estimated through ordinary least squares regression. The results showed that motivating and limiting factors had the same relative importance for farmers and advisors when deciding to adopt/recommend improved RPs. The part-worth utilities for different levels were not significantly different from each other in the advisors' rankings (<em>p</em>-values &gt;0.05), but they were significantly different (<em>p</em>-value &lt;0.05) for the farmers within the factors “Expectations”, “External support” and “Investments needed”. The highest part-worth utility was assigned to having a feasible program to implement. The lowest values corresponded to having access to public and/or private support and having to invest in regular professional advice. These findings are further evidence that the factors driving or hindering the enhancement of dairy farm management are manifold and often farm-specific.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105847"},"PeriodicalIF":1.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of early heat conditioning and dietary glutamine on productivity, immune response, and oxidative stress status in broiler chickens under heat stress conditions","authors":"Hosam M. Safaa ,&nbsp;Marwa H. Abd El-Maged ,&nbsp;Dalia A.A. El-Sayed ,&nbsp;Mohamed S. El-Kholy ,&nbsp;Ahmed Gouda","doi":"10.1016/j.rvsc.2025.105835","DOIUrl":"10.1016/j.rvsc.2025.105835","url":null,"abstract":"<div><div>Heat stress (<strong>HS</strong>) conditions in broiler farms impair antioxidant markers (total antioxidant capacity, catalase, superoxide dismutase, and malondialdehyde) and immune indices (<strong>IL1β</strong>, <strong>IFN</strong>-γ, <strong>IL10</strong>, and <strong>C3</strong>), which reflected negatively on productivity, resulting in economic losses. Therefore, we used 300 Cobb-500 broiler chicks were used to evaluate the impacts of early heat conditioning (EHC) and dietary glutamine (DG) on their productive and immunological traits. In a completely randomized factorial design (2 × 3), 300 Cobb-500 broiler chicks underwent <strong>EHC</strong> (40 vs. 32 °C) at 5 days post-hatch for 24 h and were fed <strong>DG</strong> (0, 0.4, or 0.6 %) during their first week of rearing. The impact of HS up to 35 days post-hatch was investigated (humidity temperature index, 82–91). Each treatment was replicated ten times. EHC and DG had positive effects on broiler productivity (BW at 35 days of age increased by 4.4 % and feed conversion rate values decreased by 1.9 %). Similar effects were observed for the proteinogram and metabolite constituents, lipid profiles, immune indices, antioxidant markers, and HSP70. Moreover, there were no differences in most traits between the two DG treatments (chicks fed 0.4 and 0.6 % glutamine). The results suggest synergistic effects of EHC and DG in improving broiler productivity and enhancing their physiological, antioxidant, and immune statuses under HS conditions. EHC at 5 days post-hatch for 24 h and DG supplementation during the first week of the rearing period, either separately or combined, may minimize the harmful effects of HS on broiler chickens.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105835"},"PeriodicalIF":1.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection, phylogenetic and whole genome sequence analyses of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Delta variant isolated from domestic cats (Felis catus) in Egypt","authors":"Mervat E. Hamdy ,&nbsp;Mostafa R. Zaher ,&nbsp;Rabab T. Hassanien ,&nbsp;Mary A.N. Sargious ,&nbsp;Samah F. Ali ,&nbsp;Ahmed Kayed ,&nbsp;Lotfi Allal ,&nbsp;Momtaz A. Shahin ,&nbsp;Samah Eid ,&nbsp;Mohamed E. El Zowalaty ,&nbsp;Naglaa M. Hagag","doi":"10.1016/j.rvsc.2025.105838","DOIUrl":"10.1016/j.rvsc.2025.105838","url":null,"abstract":"<div><div>Reverse zoonoses, which arise from interactions between humans and animals, are facilitated by the similarity of angiotensin-converting enzyme 2 (ACE2) cell receptors across different hosts and the high mutation rate of Severe Acute Respiratory Syndrome Coronavirus 2 <em>(SARS-CoV-2),</em> enabling transmission across species. This study aimed to explore the transmission of <em>SARS-CoV-2</em> between humans and companion animals by conducting molecular investigations on nasopharyngeal and oropharyngeal swabs from 100 domestic Cats (<em>Felis catus</em>) in Egypt which had contact with <em>SARS-CoV-2</em> positive owners from December 2021 to March 2022. Swabs were screened using real-time RT-PCR and the positive sample was further analyzed using whole-genome sequencing and phylogenetic analysis. The feline <em>SARS-CoV-2</em> strain belonged to the Delta variant showing 99.6 %–99.7 % nucleotide sequence identity to human <em>SARS-CoV-2</em> viruses collected during the same period. Amino acid mutations were observed in key regions of the spike protein (T19R, T478K, D614G, and P681R) and other genomic regions, including ORF1b, ORF1a, ORF3a, and ORF9b. Unique traits included the presence of T19R in the N-terminal domain NTD and I82T mutations in the Membrane protein. Structural analysis revealed fewer hydrogen bonds and salt bridges compared to the Delta variant viruses, showing altered spike-ACE2 interactions. Transmembrane protease serine subclass 2 (TMPRSS2) docking indicated strong binding affinity, suggesting a potential for interspecies adaptation. The present study highlights the potential occurrence of <em>SARS-CoV-2</em> spillover between humans and their companion animals. Public health precautionary and preventive measures should be implemented to reduce transmission of <em>SARS-CoV-2</em> between humans and companion animals.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105838"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extruded Spirulina and enzyme supplementation modulate hepatic proteome and metabolism in broilers","authors":"Maria P. Spínola ,&nbsp;Dina Rešetar Maslov ,&nbsp;Ivana Rubić ,&nbsp;Mónica M. Costa ,&nbsp;Vladimir Mrljak ,&nbsp;Madalena M. Lordelo ,&nbsp;André M. Almeida ,&nbsp;José A.M. Prates","doi":"10.1016/j.rvsc.2025.105824","DOIUrl":"10.1016/j.rvsc.2025.105824","url":null,"abstract":"<div><div><em>Limnospira platensis</em> (Spirulina) is prized for its nutritional and functional benefits, yet its effects on the broiler liver proteome, especially at high inclusion levels and following pre-treatments, remain underexplored. We evaluated four dietary treatments: a control (CTR) and three experimental diets, each containing 15 % Spirulina: unprocessed (SP), extruded (SPE) and extruded plus 0.21 % enzyme blend (SPEM). Hepatic proteomic profiling identified 25 proteins whose abundance differed significantly (<em>P</em> &lt; 0.05) among treatments. Proteins decreased in all Spirulina-fed groups were predominantly involved in lipid and amino-acid metabolism: hydroxymethylglutaryl-CoA lyase, S-adenosylmethionine synthase, homocysteine-binding protein, dimethylglycine dehydrogenase, acetyl-CoA acyltransferase 1, 5-aminoimidazole ribonucleotide synthase and aspartate aminotransferase. Conversely, proteins increased with Spirulina inclusion were mainly associated with carbohydrate catabolism and mitochondrial energy production: fructose-bisphosphate aldolase B, glucose-6-phosphate isomerase, UTP-glucose-1-phosphate uridylyltransferase, multiple NADH dehydrogenase subunits (α, β, 75 kDa), and the voltage-dependent anion channel protein. Importantly, the magnitude of these proteomic shifts was greatest in the SPE and SPEM groups, indicating that extrusion enhances algal cell-wall disruption and nutrient accessibility, while supplemental enzymes further liberate bioactive components. This metabolic reprogramming suggests a shift in the broiler liver toward enhanced glycolytic flux and mitochondrial oxidative phosphorylation. Such adaptations may underpin improvements in hepatic function, support more efficient growth performance and bolster overall metabolic health in poultry. To our knowledge, this is the first study to demonstrate that targeted processing of Spirulina can selectively modulate the avian liver proteome, offering a promising strategy for functional feed design.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105824"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing stifle stability in canine limb press models: A systematic review and meta-analysis","authors":"Glauco Vinício Chaves ,&nbsp;Kathrine Højte Dahl ,&nbsp;Michelle Brønniche Møller Nielsen ,&nbsp;Leonardo Augusto Lopes Muzzi ,&nbsp;James Edward Miles","doi":"10.1016/j.rvsc.2025.105826","DOIUrl":"10.1016/j.rvsc.2025.105826","url":null,"abstract":"<div><div>Limb press models have been widely used to explore aspects of canine stifle stability and to inform surgical practice. This systematic review aimed to synthesize test conditions, periarticular forces, and stability measures in canine limb press models for comparison with <em>in vivo</em> measurements. Six databases (EMBASE, Ovid MEDLINE, CAB abstracts, Agricola, Scopus, and Web of Science) were searched from inception to 17.03.2025. Peer-reviewed experimental studies using static, axially loaded, whole-limb constructs and reporting cranial tibial translation, rotation, and/or periarticular forces were eligible for inclusion. Twenty-three articles were included, reporting results from 368 healthy limbs of dogs of various breeds. Most (17/23) simulated stance during walking but landmarks for limb angulation were frequently poorly described or deviated from those used in cited studies. Cranial cruciate ligament transection caused mean cranial translation of 14.6 mm and mean internal rotation of 13.1°. Cranial translation and rotation were improved by osteotomy techniques, but data were heterogeneous, sample sizes were often small, and reporting standards inconsistent. Key data regarding quadriceps, gastrocnemius, or cranial thrust forces were extremely limited. This review identified potentially serious knowledge gaps regarding simulated muscle and periarticular forces, and concerns over limb angulation. Further evaluation of <em>ex vivo</em> models is warranted to determine the likely validity of currently reported models. If current models are shown to simulate muscle forces which do not reflect expected <em>in vivo</em> loading, this could help explain the difference between <em>in vivo</em> and <em>ex vivo</em> assessments of joint stability following surgeries such as TPLO and TTA.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105826"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144781454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ligilactobacillus salivarius and Limosilactobacillus reuteri improve growth and intestinal health in broilers via modulating gut microbiota and immune response","authors":"Neha Chavan ,&nbsp;Mahesh Gupta ,&nbsp;Krushna Bibichand Bahiram ,&nbsp;Jayant Pandurang Korde ,&nbsp;Mukund Kadam ,&nbsp;Atul Dhok ,&nbsp;Sachin Kumar","doi":"10.1016/j.rvsc.2025.105837","DOIUrl":"10.1016/j.rvsc.2025.105837","url":null,"abstract":"<div><div>The present study evaluated the effects of <em>Ligilactobacillus salivarius</em> RBL50, <em>Limosilactobacillus reuteri</em> SW23, and their mixture on growth, gut health, gut microbes, immune response, and serum lipids in broiler chickens. A total of 180 one-day-old broiler chicks were divided into five groups for six weeks: control, antibiotic (BMD), <em>L. salivarius</em> RBL50, <em>L. reuteri</em> SW23, and a combination of L. <em>salivarius</em> RBL50 + <em>L. reuteri</em> SW23. The results showed that probiotics and antibiotic groups had significantly higher average body weight (ABW) and average daily gain (ADG) compared to the control. Probiotics and antibiotics also reduced feed conversion ratio (FCR) and feed intake (ADFI), with the L. <em>salivarius</em> RBL50 + <em>L. reuteri</em> SW23 group showing the best performance. Histomorphological analysis revealed increased villus height (VH) and villus height/crypt depth ratio in the jejunum of probiotic and antibiotic groups, with RBL50 showing the highest values. Broilers treated with probiotic bacteria has higher expression of <em>claudin-1</em>, <em>ZO-1</em>, reduced total viable and coliform counts, and increased <em>Lactobacillus</em> counts in the cecum. Response to intradermal injection of Phyto-haemagglutinin-P (PHA<img>P) was significantly higher in the L. <em>salivarius</em> RBL50 and L. <em>salivarius</em> RBL50 + <em>L. reuteri</em> SW23 groups, but no differences were observed in humoral immune response. Supplementation of L. <em>salivarius</em> RBL 50 and L. <em>reuteri</em> SW23 also increased expression of anti-inflammatory cytokine interleukin-10 (IL-10) in the jejunum and spleen. In conclusion, <em>L. salivarius</em> RBL50 and L. <em>reuteri</em> SW23 can improve growth, gut health, and immune response, making them effective and safe alternatives to antibiotic growth promoters.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105837"},"PeriodicalIF":1.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and risk factors of canine leishmaniasis in Mediterranean countries: A systematic review and meta-analysis (2000–2024)","authors":"Ilham Ferdes ,&nbsp;Bachir Medrouh ,&nbsp;Ahcene Hakem ,&nbsp;Ismail Lafri","doi":"10.1016/j.rvsc.2025.105836","DOIUrl":"10.1016/j.rvsc.2025.105836","url":null,"abstract":"<div><div>Caused by <em>Leishmania infantum</em>, canine leishmaniasis (CanL) is endemic in the Mediterranean basin and can threaten public health. We conducted a systematic review and meta-analysis to summarize and standardize existing knowledge about CanL in the Mediterranean by determining global prevalence recorded in the last decades and the potential risk factors associated. According to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyzes) protocol, systematic searches were conducted in three databases: PubMed, Google Scholar, and Science Direct. Between 2000 and 2024, ninety-six eligible publications were selected, and statistically analyzed. The pooled overall CanL seroprevalence was 16.12 % (14,351/134752, 95 % Cl: 13.27–19.18). The highest prevalence rate of 21.73 % (95 % Cl: 15.49–28.69) was reported in the African continent and followed by Southern and Middle Eastern countries. Age, sample size, clinical status and dog's activity were revealed as risk factors associated with CanL (<em>p</em> &lt; 0.0001). The highest prevalence was observed in older dogs (&gt;1 year) (18.72 %; 95 % Cl: 12.73–25.54), sample size &lt;100 (26.82 %, 95 % Cl: 16.56–37.11), presence of clinical signs (30.39 %, 95 % CI: 18.53–43.65) and farming dogs (23.89 %, 95 % Cl: 12.50–37.36). However, no statistical significance was found with sex, breed, WHO regions and the diagnostic methods. These interesting findings are needed to improve the monitoring and management of disease, especially in low-income countries, and to establish crucial control strategies to eradicate the disease across the Mediterranean area and eliminate the transmission risk to humans.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105836"},"PeriodicalIF":1.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecology of pet birds and epidemiology of multidrug resistant Escherichia coli and Staphylococcus spp. and resistant patterns in Budgerigar","authors":"Md. Saddam Hossain ,&nbsp;Md. Kaisar Rahman ,&nbsp;Chandan Nath ,&nbsp;Mahfuja Akter ,&nbsp;Helal Uddin ,&nbsp;Ariful Islam ,&nbsp;Kohei Makita ,&nbsp;Mohammad Mahmudul Hassan","doi":"10.1016/j.rvsc.2025.105825","DOIUrl":"10.1016/j.rvsc.2025.105825","url":null,"abstract":"<div><div>Pet birds are most popular as pets in Bangladesh, with budgerigars being the most common species. However, the emergence of antimicrobial resistance poses a growing threat to both pet birds and their owners. This study aims to investigate the ecology of pet birds and the epidemiology of multidrug resistant (MDR) <em>Escherichia coli</em> and <em>Staphylococcus</em> spp. in pet birds. Data were collected from interviews with 49 pet bird farms using a pretested questionnaire, and cloacal swab samples were collected from 220 budgerigars between December 2016 and June 2017. Standard microbiological procedures were employed for bacterial isolation and identification. Antimicrobial susceptibility was tested using micro-disc diffusion techniques for 9 antibiotics. Budgerigar birds were found to be 24.7 % of all pet species. The prevalences of <em>E. coli</em> and <em>Staphylococcus</em> spp. were 22.3 % and 18.2 %, respectively. All the <em>E. coli</em> (<em>n</em> = 49) isolates displayed resistance to amoxicillin, sulfamethoxazole, trimethoprim, and cefixime, with the lowest resistance observed against ciprofloxacin (6.1 %). For <em>Staphylococcus</em> spp., all the isolates (<em>n</em> = 40) were resistant against enrofloxacin and gentamycin, with the lowest resistance to ciprofloxacin and azithromycin (5 %). Significant factors associated with MDR included female budgerigars with poor body condition score (BCS), which was linked to a significantly higher prevalence of MDR <em>E. coli</em>. For MDR S<em>taphylococcus</em> spp., young age emerged as a significant factor (<em>p</em> &lt; 0.005). To mitigate the risk of public health implications associated with multidrug resistant bacteria, there is a need to reduce the indiscriminate use of antibiotics in pet birds.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105825"},"PeriodicalIF":1.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential immunoprophylactic use of a multi-epitope protein of CD4+ and CD8+ T cells against visceral leishmaniasis in Balb/c mice","authors":"Scarleth Silva Costa ,&nbsp;Naianda Rezende Ribeiro ,&nbsp;Luiza dos Reis Cruz ,&nbsp;Gabriel José Lucas Moreira ,&nbsp;Vivian Tamietti Martins ,&nbsp;Daniela Pagliara Lage ,&nbsp;Danniele Luciana Vale ,&nbsp;Camilly Vitória Silva Costa ,&nbsp;Nathalia Coral Galvani ,&nbsp;Gabriel Paulino Luiz ,&nbsp;Maria Eduarda de Oliveira ,&nbsp;Ricardo Andrez Machado-de-Ávila ,&nbsp;Ana Maria Ravena Severino Carvalho ,&nbsp;Níbia Mariana Eleutério ,&nbsp;Ana Paula Salles Moura Fernandes ,&nbsp;Eduardo Antonio Ferraz Coelho ,&nbsp;Bruno Mendes Roatt ,&nbsp;Daniel Menezes-Souza ,&nbsp;Mariana Costa Duarte","doi":"10.1016/j.rvsc.2025.105820","DOIUrl":"10.1016/j.rvsc.2025.105820","url":null,"abstract":"<div><div>Diseases caused by parasites of the genus <em>Leishmania</em> are considered neglected infections. Visceral leishmaniasis (VL) is the most severe form of the disease, with a high annual incidence worldwide. In Brazil, dogs play an essential role as reservoirs, exhibiting a high concentration of parasites on their skin and no clinical signs, making them potential transmitters of the disease. Given the need for an efficient method to reduce the incidence of the disease, this study aimed to develop a gene encoding a chimeric protein with multiple epitopes that target both CD4⁺ and CD8⁺ T lymphocytes. Together with the MPLA adjuvant, the chimeric protein has the potential to compose a vaccine formulation against canine visceral leishmaniasis. Using an immunoinformatics analysis tool, the prediction of specific epitopes for CD4⁺ and CD8⁺ T lymphocytes was performed on sequences of hypothetical proteins previously identified in the immunoproteome of <em>Leishmania (Leishmania) infantum</em> parasites. The vaccine formulation was evaluated in Balb/c mice, and the chimeric protein, in association with the adjuvant, induced a protective response with a polarized T_H1 profile against the <em>Leishmania</em> parasite, characterized by high levels of IFN-γ, TNF-α, and low levels of IL-10. Additionally, the vaccinated animals showed a significant reduction in parasite burden in the liver, spleen, and bone marrow and draining lymph nodes of the paws compared to the control groups. Thus, the chimeric protein described here has the potential to be used in a vaccine formulation against canine leishmaniasis.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"194 ","pages":"Article 105820"},"PeriodicalIF":1.8,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}