Research in veterinary science最新文献

{"title":"Escherichia coli strains from wild birds in Türkiye: Antibiotic resistance, virulence factors and phylogroups","authors":"Doğancan Yarım,&nbsp;Emre Karakaya,&nbsp;Fuat Aydın,&nbsp;Seçil Abay","doi":"10.1016/j.rvsc.2025.106045","DOIUrl":"10.1016/j.rvsc.2025.106045","url":null,"abstract":"<div><div>This study aimed to isolate and identify <em>Escherichia coli</em> (<em>E. coli</em>) from the feces of wild bird species and to determine antibiotic susceptibilities, phylogroups, and virulence genes in the isolates obtained.</div><div>For this purpose, a total of 575 fecal samples from 100 Eurasian Tree Sparrow, 250 Eurasian Jackdaw, 156 Eurasian Magpie, 4 Short-toed Snake-Eagle, 60 Rock dove, and 5 Long-legged Buzzard were used. <em>E. coli</em> isolation was performed using direct inoculation on MacConkey agar. The isolates were identified through phenotypic tests, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS), and Polymerase Chain Reaction (PCR). The susceptibility of the isolates to nine antibiotics was determined by the disk diffusion method. Phylogroups and the presence of virulence genes were determined by Clermont typing and the multiplex PCR (mPCR) methods, respectively. Among 575 feces samples, <em>E. coli</em> was isolated from 37 (6.43 %). The highest antibiotic resistance was found in ampicillin, azithromycin, and trimethoprim-sulfamethoxazole at 35.1 %. Moreover, 43.2 % of the <em>E. coli</em> isolates were detected as Multidrug-Resistant (MDR), and these isolates exhibited 13 different MDR patterns by demonstrating resistance to three to eight various antibiotic classes. While the most common phylogroup detected among isolates was A (18.9 %), none belonged to phylogroups C and F. 72.9 % had at least one virulence gene. The most frequently detected virulence gene was <em>stx1</em> + <em>stx2</em> (32.4 %), and all isolates were negative for <em>bfpA</em> and <em>lt</em> genes.</div><div>In conclusion, considering the role of birds of prey in the ecological balance, the data obtained in this study suggest that wild birds may contribute to the spread of pathogenic and antibiotic-resistant <em>E. coli</em> strains globally.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106045"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of endoparasite infections in the European hedgehog (Erinaceus europaeus) and association with rehabilitation outcome in wildlife centres in Northwest France","authors":"Paula Yagüez I. López-Jurado ,&nbsp;Katie Beckmann ,&nbsp;Rob Kelly","doi":"10.1016/j.rvsc.2025.106034","DOIUrl":"10.1016/j.rvsc.2025.106034","url":null,"abstract":"<div><div>The European hedgehog (<em>Erinaceus europaeus</em>) is prevalent across Western Europe, with many individuals admitted to wildlife rehabilitation centres. Parasitic infections, particularly helminth parasites, may threaten rehabilitation success. This study investigated respiratory and gastrointestinal endoparasite prevalence, risk factors, and associations with survival in 300 hedgehogs admitted to four rehabilitation centres in northwestern France (May 2023–May 2024). Faecal samples were analysed using either the modified McMaster technique (Oise) or direct smear (Seine-Maritime, Loir-et-Cher, Essonne). Overall, 58.3 % of hedgehogs carried at least one endoparasite. The most common were <em>Capillaria</em> species group (43.6 %) and <em>Crenosoma striatum</em> (25.7 %), followed by <em>Brachylaemus erinacei</em> (11 %) and <em>Cycloisospora</em> species group (7.3 %). Co-infection was common; 35 % of infected individuals harboured both <em>Capillaria</em> spp. and <em>C. striatum</em>. Younger hedgehogs showed lower prevalence but higher parasite burdens, suggesting reduced resistance. <em>Capillaria</em> spp. prevalence peaked in winter across all age groups. Multivariable analysis identified age, weight, season, and admission reason as significant risk factors for parasitism. Importantly, detection of <em>C. striatum</em> significantly increased mortality risk during rehabilitation (OR = 3.44, <em>p</em> = 0.0002). These findings highlight the need for targeted parasite screening and treatment protocols for specific risk groups, for example for juveniles and individuals with clinical signs. Coprological analysis offers a practical, non-invasive tool for parasite detection in resource-limited rehabilitation settings. This is the first study to report endoparasite prevalence in live hedgehogs in France using coprological methods and to demonstrate a statistical association between <em>C. striatum</em> infection and mortality in rehabilitation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106034"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145766055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of a novel porcine reproductive and respiratory syndrome virus strain inducing biphasic temperature fluctuations in Tianjin, northern China","authors":"Jia Wang ,&nbsp;Yi-Han Lu ,&nbsp;Bo-Han ,&nbsp;Hai-Tao Yang ,&nbsp;Xin-lei Li ,&nbsp;Wen Sun ,&nbsp;Shi-Qin Zhang ,&nbsp;Zhuo-Ran Miao ,&nbsp;Xue-Jiao Cheng ,&nbsp;Chun-Xue You ,&nbsp;Ying-Feng Sun","doi":"10.1016/j.rvsc.2025.106037","DOIUrl":"10.1016/j.rvsc.2025.106037","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) has imposed significant economic burdens on the global swine industry. The majority of PRRSV strains can induce high fever at various stages of pig development. However, the PRRSV strain that triggers the biphasic temperature phenomenon remains relatively rare. In this study, a novel PRRSV variant, designated SJ0107, was isolated from local pig farms in Tianjin, Northern China, in 2024. Recombination analysis reveals that SJ0107 is a recombinant PRRSV strain resulting from the interaction between lineage 1.8 and lineage 8.3, with four potential recombination breakpoints located in Nsp2 (nt 766/1997), Nsp4 (nt 5413), and Nsp9 (nt 7750). Animal challenge experiments conducted on four-week-old piglets demonstrated that SJ0107 can induce rare initial hypothermia symptoms within 5 days post-inoculation (dpi). Subsequently, the body temperature returned to normal, and high-grade fever symptoms emerged at 11 dpi, lasting for one week before normalizing again. Meanwhile, severe histopathological lung lesions were also detected in the pathological sections. In conclusion, the findings reveal that the SJ0107 strain undergoes a dual-phase temperature alteration, which not only complicates traditional diagnostic criteria reliant on persistent fever but also heightens the risk of misdiagnosis in the initial phases of infection. These insights underscore the necessity of revising surveillance protocols for emerging PRRSV variants that exhibit atypical pathogenic characteristics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106037"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A field observational study evaluating antemortem inspection as a predictive tool for postmortem lesion findings at a Portuguese pig abattoir","authors":"Márcia Santos Nunes ,&nbsp;Abbey Olsen ,&nbsp;Rui Pedro Cordeiro ,&nbsp;Madalena Vieira-Pinto","doi":"10.1016/j.rvsc.2025.106043","DOIUrl":"10.1016/j.rvsc.2025.106043","url":null,"abstract":"<div><div>Risk-based inspection of pigs at the abattoir depends on accurately identifying high-risk animals before slaughter. Food Chain Information (FCI) provides data on farm management, animal health, and treatments that can support this process; however, its limited completeness and reliability highlight the need for complementary approaches. Antemortem (AM) conditions may serve as practical predictors of postmortem (PM) lesions, supporting the development of risk-based inspection systems. This study investigated the potential of AM inspection in detecting PM lesions in finisher pigs, slaughtered at a commercial abattoir in Portugal. Data were collected from 62 batches (<em>n</em> = 9542 pigs) for a cross-sectional study, and 245 pigs for a case control study. Detection of coughing, skin lesions, and growth retardation was significantly higher when pigs were inspected from inside the pens, compared with unloading or outside the pens observations. At batch-level coughing at AM inspection was associated with a higher number of PM lesions, particularly pneumonia (OR = 1.08) and pleurisy (OR = 1.03). At animal-level, AM conditions showed consistent associations with PM lesions, including coughing with pneumonia (OR = 6.7), and lung lesion (OR = 6.2), lameness with arthritis (OR = 14.9) and growth retardation with several PM lesions. These findings are consistent with those reported in other countries and confirm that specific AM conditions can reliably predict certain PM lesions, contributing to the refinement of risk-based inspection approaches in Portugal.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106043"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145895943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of detection method of chicken infectious anemia virus based on CRISPR/Cas12a system","authors":"Chenchen Sheng ,&nbsp;Jingfang Wang ,&nbsp;Mengyuan Tan ,&nbsp;jingwen Zhang ,&nbsp;Mengran Sun ,&nbsp;Jiumeng Sun ,&nbsp;Ying Shao ,&nbsp;Jian Tu ,&nbsp;Liangqiang Zhu ,&nbsp;Xiangjun Song","doi":"10.1016/j.rvsc.2025.106046","DOIUrl":"10.1016/j.rvsc.2025.106046","url":null,"abstract":"<div><div>Chicken Infectious Anemia Virus (CIAV) causes chicken infectious anemia, characterized by anemia and immune dysfunction. The rapid dissemination of this virus is generating substantial economic consequences for poultry producers.</div><div>The CRISPR/Cas12a system is widely used for virus detection through crRNA-guided target recognition and the paracrine activity of Cas12a. To enable rapid and highly sensitive detection of Chicken Infectious Anemia Virus (CIAV), a CRISPR-Cas12a-based fluorescence assay was refined. Through optimization of the CRISPR/Cas12a system and integration of enzymatic recombinase amplification (ERA), the assay achieved a detection limit of 1 copy/μL, demonstrating its significant utility for CIAV diagnostics. In addition, a CRISPR/Cas12a lateral flow assay was developed and optimized, achieving a sensitivity of 10^3 copies/μL for the rapid and visual detection of target analytes. This technique exhibits high specificity for CIAV, showing no cross-reactivity with other chicken viruses. Overall, the system enables rapid CIAV detection with cost-effective equipment, making it suitable for virus monitoring.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106046"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145918358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remedial effects of extracellular polymeric substances (EPS) from Arthrospira platensis against Di-n-butyl phthalate (DBP) toxicity on gilthead seabream (Sparus aurata)","authors":"Amira Bemri ,&nbsp;Fethi Jebali ,&nbsp;Jihene Ammar ,&nbsp;Tahar Gharred ,&nbsp;Jamel Jebali ,&nbsp;Hamadi Guerbej ,&nbsp;Hatem Ben Ouada ,&nbsp;Zied Bouraoui","doi":"10.1016/j.rvsc.2025.106041","DOIUrl":"10.1016/j.rvsc.2025.106041","url":null,"abstract":"<div><div>Di-n-butyl phthalate (DBP) is an organic compound of phthalate, commonly used in the production of various plastic materials and its detrimental effects on marine ecosystems and human health have a great concern. Bioactive compounds derived from microalgae have numerous biological and pharmacological activities and their remedial role in alleviating the adverse effects of toxicity induced by environmental and other agents has also been reported. Thus, in this work, we carried out studies to ascertain if exopolysaccharides (EPS) extracted from <em>Arthrospira platensis</em> (Cyanobacteria) would ameliorate DBP-induced toxicity in gilthead seabream (<em>Sparus aurata</em>), used as model organism, focusing on growth performance, oxidative stress, neurotoxicity, histopathological changes in liver and intestine, and muscle nutritional quality. Firstly, fish in triplicate were fed separately a supplemented diet with 0 % (control group) and 6 % of EPS (EPS group) for 21 days. Consequently, each group were intraperitoneally exposed to DBP at a concentration of 85 μg /kg body weight. The results showed that DBP impaired growth, induced antioxidant-oxidative stress imbalance and caused genotoxicity and liver and intestine damage. Also, DBP disrupted muscle proximate composition and fatty acid profiles. Interestingly, feeding the DBP-exposed fish with EPS partially restored, histopathological damage in liver and intestine, antioxidant markers, lipid balance, notably improving the ω3/ω6 ratio, likely through EPS's antioxidant and metabolic regulatory effects.</div><div>Overall, this study offered further insights on the antioxidative and chemopreventive properties of exopolysaccharides (EPS) extracted from <em>Arthrospira platensis</em> to modulate fish health and to enhance growth and protect against DBP-induced toxicity.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106041"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Brucella spp. in compost-bedding pack barn and its relationship with infections in dairy cows during the transition period","authors":"Marcilene Daniel Damasceno ,&nbsp;Maysa Serpa Gonçalves ,&nbsp;Maria Eduarda de Souza Teixeira Campos ,&nbsp;Amanda Carvalho Rosado Ferreira ,&nbsp;Dircéia Aparecida Costa Custódio ,&nbsp;Eduarda Moraes Magossi Silva ,&nbsp;Vivian Aparecida Malta ,&nbsp;Alice Gonçalves dos Reis ,&nbsp;Bruno Borges Silva ,&nbsp;Ana Clara de Serpa Carvalho ,&nbsp;Júlia Lima Paz ,&nbsp;Pedro Felipe Rodrigues e Oliveira ,&nbsp;Bruno Campos de Carvalho ,&nbsp;Guilherme Nunes Souza ,&nbsp;Carine Rodrigues Pereira ,&nbsp;Elaine Maria Seles Dorneles ,&nbsp;Alessandro de Sá Guimarães","doi":"10.1016/j.rvsc.2025.106047","DOIUrl":"10.1016/j.rvsc.2025.106047","url":null,"abstract":"<div><div>Compost-bedded pack barn (CBP) is a used intensive system for dairy cow confinement, however, information about the possible role of the bed in the maintenance and transmission of pathogens among housed animals is still scarce. This study investigates the presence of <em>Brucella</em> spp. in dairy cows in the transition period housed in CBP and the detection of the pathogen in the CBP. The analyzes were conducted using samples of CBP and samples from cows up to 45 days post-partum from 20 different farms in Goiás and Minas Gerais states, Brazil. Animals were tested for the presence of anti-<em>Brucella</em> antibodies. Samples of vaginal swab, endometrial sample and CBP were tested for the presence of <em>Brucella</em> spp. using conventional PCR targeting the <em>bscp31</em> gene, as a screening test, and the real-time PCR targeting the <em>IS711</em> gene, as confirmatory test. A total of 16 [16/20 (80 %)] properties exhibited at least one positive animal in at least one test (serology or molecular tests), and four [4/20 (25 %)] of these properties had at least one positive animal in at least two tests performed. Additionally, three CBP [3/44 (6.88 %)] showed PCR positive result to <em>Brucella</em> spp. The results demonstrated the presence of <em>Brucella</em> spp. in sample collected from dairy cows in transition period housed in CBP and the bed of the system, suggesting the CBP may have a role in brucellosis transmission in the system, by favoring the spread of the agent and transmission to other housed cows.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106047"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The availability of outdoor spaces enhances social play in dairy cows","authors":"Alfredo Di Lucrezia ,&nbsp;Daria Lotito ,&nbsp;Valeria Iervolino ,&nbsp;Pietro Lombardi ,&nbsp;Biagio D'Aniello ,&nbsp;Vincenzo Mastellone","doi":"10.1016/j.rvsc.2025.106048","DOIUrl":"10.1016/j.rvsc.2025.106048","url":null,"abstract":"<div><div>Social behaviors are widely recognized as valuable indicators of animal welfare. This study examined the behavioral profiles of two groups of cows: one with daytime access to an open outdoor area and one housed exclusively in a free-stall barn. Observations were conducted when both groups were in the same indoor environment, allowing for a direct comparison of their behavioral expressions. Cows with outdoor access engaged significantly more in social play, while no statistically significant differences were observed for other social behaviors such as allogrooming, social rubbing, or submission/avoidance. Since play behavior typically emerges under favorable psychological conditions, these findings suggest that outdoor access during the day contributes to enhanced psychological wellbeing, with positive effects that persist even when cows return to the confined barn setting. In contrast, negative social interactions such as submission/ avoidance were expressed at similar levels across both groups, indicating that access to open space may not be sufficient to mitigate all forms of social stress.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106048"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of monensin and alternative feed additives on productive performance and rumen fermentation in Zebu beef cattle: A systematic review and meta-analysis","authors":"Ingrid Guidi Ribeiro ,&nbsp;Ana Clara Nogueira Gonçalves ,&nbsp;Letícia Gobbo Oliveira ,&nbsp;Guilherme Lobato Menezes ,&nbsp;Ângela Maria Quintão Lana ,&nbsp;Rafahel Carvalho Souza ,&nbsp;Alan Figueiredo de Oliveira","doi":"10.1016/j.rvsc.2026.106072","DOIUrl":"10.1016/j.rvsc.2026.106072","url":null,"abstract":"<div><div>Zebu cattle predominate in tropical beef systems, but no meta-analysis has compared monensin with alternative additives specifically in Zebu cattle. This meta-analysis aimed to compare the effects of monensin and alternative feed additives on productive performance and rumen fermentation in Zebu beef cattle. Forty-seven studies (408 comparisons) were included. We performed a random-effects meta-analysis to estimate mean differences (MD) with 95% confidence intervals and explored heterogeneity using subgroup analyses and meta-regression. Average daily gain (ADG) was greater in animals receiving natural additives (+5.01%; MD = 0.063; CI: 0.048 to 0.078) or probiotics (+5.03%; MD = 0.083; CI: 0.021 to 0.145) than in those receiving monensin. In contrast, ADG was lower in animals treated with virginiamycin (−3.42%; MD = −0.042; CI: −0.073 to −0.010) or lasalocid (−14.5%; MD = −0.076; CI: −0.081 to −0.072). These increases in ADG with natural additives and probiotics could potentially shorten finishing time under typical feedlot conditions. Dry matter intake (DMI) was higher in animals receiving monensin plus virginiamycin, natural additives, virginiamycin, monensin plus narasin, probiotics, narasin, and monensin plus probiotics compared with monensin alone. Feed efficiency (FE) was higher in animals supplemented with monensin plus virginiamycin (+2.05%; MD = 0.003; CI: 0.0003 to 0.006) but lower in those receiving virginiamycin alone (−9.90%; MD = −0.014; CI: −0.016 to −0.011) compared with monensin. Methane emissions did not differ among treatments, indicating similar effects on enteric methane production. Natural additives and probiotics increased ADG, but the diversity of compounds and inclusion levels limits identifying which specific components drive the response. Overall, monensin remains a consistent additive for improving FE in Zebu cattle, whereas combining it with virginiamycin resulted in even greater FE and may represent a useful strategy.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106072"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological and epidemiological assessment of cranial crassicaudiasis in stranded cetaceans from the Canary Islands (1999–2024)","authors":"Zuleima Suárez-González ,&nbsp;Antonio Fernández ,&nbsp;Jorge F. González ,&nbsp;Noelia Salgado-Jiménez ,&nbsp;Ignacio Molpeceres-Diego ,&nbsp;Paula Alonso-Almorox ,&nbsp;Eva Sierra","doi":"10.1016/j.rvsc.2025.106035","DOIUrl":"10.1016/j.rvsc.2025.106035","url":null,"abstract":"<div><div><em>Crassicauda</em> nematodes in cetaceans infect various organs, including the genitourinary system, subcutaneous tissues, circulatory system, and cranial structures such as the pterygoid sacs. In the cranial region, they may induce osteolytic lesions, potentially leading to neurological impairment, disorientation, and contributing to stranding and death. This study investigated the epidemiology and pathology of cranial crassicaudiasis in cetaceans stranded in the Canary Islands between 1999 and 2024, with emphasis on associated central nervous system (CNS) lesions. The pterygoid sacs of 438 cetaceans were systematically examined, and <em>Crassicauda</em> sp.-induced sacculitis was detected in 49 individuals (11.2 %) of the family Delphinidae. Prevalence was highest in Risso's dolphins (<em>Grampus griseus</em>; 11/18, 61.1 %), and juvenile and subadult animals were more frequently affected (24/49, 49 %), with no sex-related differences. Molecular characterization targeting <em>COX1</em> and <em>ITS2</em> gene regions was performed on nematodes from an Atlantic spotted dolphin (<em>Stenella frontalis</em>) and a Risso's dolphin, confirming <em>C. grampicola</em> in both species. Histopathology in selected cases (26; 5 %) revealed epithelial hyperkeratosis, desquamation, and mild lymphoplasmacytic inflammation in parasitized pterygoid sacs. CNS inflammation was observed in 16/49 cases (32.6 %), including co-infections with <em>Brucella</em> spp., <em>Cetacean Morbillivirus</em> (CeMV), Herpesvirus and <em>Nocardia otitidiscaviarum</em>. No statistically significant association was found between cranial crassicaudiasis and CNS lesions. These findings highlight cranial crassicaudiasis as a relevant pathological condition in stranded cetaceans, suggesting that parasite-associated lesions may contribute to morbidity and mortality, and provide the first molecular confirmation of <em>C. grampicola</em> in <em>S. frontalis</em>, contributing novel sequences to GenBank.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106035"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}