Research in veterinary science最新文献

{"title":"Evaluation of Scalibor® deltamethrin collar chronic exposure in dogs","authors":"Larissa T.P. Rodrigues ,&nbsp;Andriele F. Qualhato ,&nbsp;Carolle V.M. Sprícigo ,&nbsp;Kelly C.A. Arantes ,&nbsp;Rayanne H.S. da Silva ,&nbsp;Alexandre C.M. Barros ,&nbsp;Monica C. Vicente ,&nbsp;Murilo R. Souza ,&nbsp;Luiza G.F. de Paula ,&nbsp;Danilo Roman-Campos ,&nbsp;Artur S. Miranda ,&nbsp;Danieli B. Martins ,&nbsp;Ana F.M. Botelho","doi":"10.1016/j.rvsc.2024.105502","DOIUrl":"10.1016/j.rvsc.2024.105502","url":null,"abstract":"<div><div>Deltamethrin is a pesticide used worldwide with several applications in the control of nuisance arthropods in homes, agriculture, against ectoparasites in dogs and livestock. Recent studies indicate that deltamethrin can induce cardiotoxicity. Therefore, this study aimed to evaluate the toxicity of deltamethrin in dogs through exposure to commercial collars over 8 months. Twenty healthy dogs were selected, 9 males and 11 females, weighing between 10 and 15 kg and aged between 1 and 8 years, with no history of intentional exposure to pyrethroids. Clinical, cardiological, and laboratory evaluations were performed monthly. The results demonstrate that exposure to deltamethrin 4 % via the dermal route, through a commercial collar, was safe for 8 months, with preserved cardiac electrocontractile function, assessed by electrocardiogram and echocardiogram. In addition, tracking of plasma biochemical markers did not indicate signs of cardiac, renal, or hepatic injury. Although deltamethrin 4 % collars demonstrated an acceptable safety profile regarding cardiovascular and laboratory evaluations, the occurrence of dermatological reactions in 25 % of the animals, which is a significant proportion, highlights the need for careful monitoring of dermatological reactions. Future investigations with different concentrations and longer periods are recommended to achieve a more comprehensive understanding of the long-term effects of deltamethrin, improving its safe application in veterinary medicine and promoting animal and human health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105502"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence of lagomorpha coccidiosis from 1951 to 2024: A systematic review and meta-analysis","authors":"Huang-Liang Chen,&nbsp;Qi-Tao Zhang,&nbsp;Rui Qi","doi":"10.1016/j.rvsc.2024.105519","DOIUrl":"10.1016/j.rvsc.2024.105519","url":null,"abstract":"<div><div>The global prevalence of coccidia infection in lagomorphs and potential risk factors were investigated through a meta-analysis of 149 studies published between 1951 and 2024. The pooled prevalence of Eimeriidae, Sarcocystidae and Cryptosporidiidae was found to be 66.0 %, 8.0 % and 6.1 %, respectively. Rabbits and hares exhibited the highest prevalence of Eimeriidae at 63.1 % and 71.8 %, while pikas had the highest prevalence of Cryptosporidiidae at 17.1 %. Countries located in the southern hemisphere exhibited the highest prevalence of Cryptosporidiidae (11.4 %). The African Region had the highest prevalence of Eimeriidae (73.3 %) and Sarcocystidae(14.6 %), while the Eastern Mediterranean Region had the highest prevalence of Cryptosporidiidae(13.6 %). Juvenile rabbits exhibited the highest prevalence of Eimeriidae (67.4 %), while senior Rabbit exhibited the highest prevalence of Sarcocystidae(17.1 %) and Cryptosporidiidae(13.1 %). The prevalence of coccidia in intensively farmed lagomorphs showed a slight upward trend over time, nearly reaching statistical significance (R2 = 7.88 %, coefficient = 0.019, <em>P</em> = 0.127). This suggests that the current intensive farming practices may be losing effectiveness in controlling coccidia infections, which is concerning. Among Eimeriidae in lagomorphs, <em>Eimeria coquelinae</em> showed the highest prevalence (68.4 %), while <em>E. magna</em> was most frequently reported. For Sarcocystidae, <em>Besnoitia</em> spp. had the highest prevalence (31.3 %), with <em>Toxoplasma gondii</em> being most frequently reported. <em>Cryptosporidium cuniculus</em> was both the most prevalent and frequently reported Cryptosporidiidae species (6.9 %), suggesting it may be a major Cryptosporidiidae pathogen in lagomorphs. These findings highlight the differences in prevalence of various Eimeriida species among different lagomorpha genera. This diversity underscores the complex relationships between specific parasites and their host species, contributing to a more nuanced understanding of coccidiosis epidemiology in lagomorphs worldwide.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105519"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey on canine hookworms: Knowledge, perceptions and practices among U.S. veterinarians","authors":"Sara J. Ochoa ,&nbsp;Gregg A. Hanzlicek ,&nbsp;Kamilyah R. Miller ,&nbsp;Justin Kastner ,&nbsp;Jeba R.J. Jesudoss Chelladurai","doi":"10.1016/j.rvsc.2024.105501","DOIUrl":"10.1016/j.rvsc.2024.105501","url":null,"abstract":"<div><div>Canine hookworms (<em>Ancylostoma caninum</em>) are highly prevalent in pet dogs in the United States of America (U.S.). They pose a significant health risk to infected dogs and are also a potential risk to humans as zoonotic agents. Veterinarians play a crucial role in managing this threat through the diagnosis and treatment of infections in pet dogs. However, the rising incidence of anthelmintic resistance in U.S. hookworm populations necessitates increased awareness among veterinarians. To determine U.S. veterinarian's knowledge, perceptions and practices about canine hookworms, a cross-sectional online survey was administered between February and December 2022. 208 veterinarians from 43 states participated in the study. Only 65 % of veterinarians reported awareness of hookworm prevalence. Hookworm infected dogs were encountered more frequently in the South than in Western states. Veterinarians perceived that hookworms were present in all breeds without breed predilection followed by mixed breeds, pit bulls and hound breeds. Most respondents reported that infections were rarely symptomatic, highlighting the importance of diagnostic testing. In-clinic diagnostic modalities were most preferred. Fenbendazole and pyrantel pamoate were the most commonly used anthelmintics. Most veterinarians followed up on infected dogs but retesting time after treatment varied. Awareness of multi-drug resistant hookworms was high but encountering persistent hookworm cases was lower. Veterinarians frequently educated clients about hookworms and prevention, but less about the zoonotic potential. This study provides insights for improving awareness, diagnostics, treatment protocols, and client education regarding canine hookworms in the U.S.. Overall, veterinarians play an active role in hookworm prevention and control.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105501"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of prostatic androgen receptor and alpha-smooth muscle actin in castrated and non-castrated dogs","authors":"Sameh M. Farouk ,&nbsp;Amal A.M. Ahmed ,&nbsp;Mohamed A. Hashem ,&nbsp;Safa H. Badran","doi":"10.1016/j.rvsc.2025.105563","DOIUrl":"10.1016/j.rvsc.2025.105563","url":null,"abstract":"<div><div>The prostate, the only accessory sex gland in dogs, is androgen-dependent and has a vital influence on male fertility. Either for therapeutic or preventative purposes, orchidectomy is performed in both human and animal, especially for androgen-dependent disorders. Thereby, the current study aimed to evaluate the immunohistochemical characteristics of the canine prostate gland after unilateral and bilateral orchiectomy. Primarily, twenty-one healthy male dogs of 18–20 kg and 1–2 years were divided into three main groups; control, unilateral castrated and bilateral castrated groups. The last two groups were subdivided, based on post castration time, into three subgroups; 10, 20, and 30 days. The prostatic tissues were harvested from all experimental groups for immunohistochemical studies. The results revealed progressive significant alterations in the prostate gland immunostaining affinity after bilateral castration as time passed; gradual reduction in the androgen receptors and α-SMA immunoexpression was observed. On the contrary, the unilateral procedure conserves the prostatic tissue nearly similar to the control. It was concluded that, however, immunohistochemical characteristics of prostatic tissue were maintained without detectable changes after unilateral castration, there was a marked ongoing decrease of androgen receptors and α-SMA immunoreactivity post bilateral castration procedure.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105563"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143146623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring endosymbionts and pathogens in Rhipicephalus sanguineus and Ctenocephalides felis felis with Oxford Nanopore Technology","authors":"Luisa Páez-Triana ,&nbsp;David Martinez ,&nbsp;Luz H. Patiño ,&nbsp;Marina Muñoz ,&nbsp;Claudia M. Sandoval-Ramírez ,&nbsp;Juan Carlos Pinilla León ,&nbsp;Juan David Ramirez","doi":"10.1016/j.rvsc.2025.105562","DOIUrl":"10.1016/j.rvsc.2025.105562","url":null,"abstract":"<div><div>Fleas and ticks play a crucial role in public health as vectors of multiple diseases affecting humans and animals. Several rickettsial pathogens and endosymbionts are transmitted by fleas and ticks. Therefore, understanding this group of microorganisms is essential for fully grasping the spectrum of pathogens transmitted by vectors and the interactions between endosymbiotic microorganisms and their hosts. This study evaluated the presence and diversity of <em>Rickettsiales</em> species in fleas and ticks collected from the Santander department in Colombia. For the methodology a 16S gene amplification approach through Oxford Nanopore sequencing technologies in <em>Rhipicephalus sanguineus</em> and <em>Ctenocephalides felis felis</em> was used. Our findings revealed the presence of multiple pathogenic and endosymbiotic microorganisms, particularly from the <em>Rickettsia</em> and <em>Wolbachia</em> groups. We observed a clear association between <em>Rickettsia</em> species and ticks, while <em>Wolbachia</em> was predominantly found in fleas. Additionally, other important microorganisms were identified, including <em>Anaplasma phagocytophilum</em>, <em>Rickettsia conorii</em>, and different strains of <em>Wolbachia</em> that serve as endosymbionts in various arthropods. These results underscore the importance of fleas and ticks in the transmission of both pathogenic and endosymbiotic microorganisms. The distinct patterns of association between specific pathogens and vectors provide insight into their transmission dynamics. Identifying pathogens such as <em>Anaplasma phagocytophilum</em> and <em>Rickettsia conorii</em> further highlights the need for continued research into vector-borne diseases in Colombia. Understanding the interactions between endosymbionts and pathogenic microorganisms in these vectors could lead to the development of more effective strategies for controlling diseases transmitted by fleas and ticks.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105562"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143337940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of delayed milk ejection on mammary gland health and milking performance in dairy cows: A systematic review and meta-analysis","authors":"Mohammad Dahl ,&nbsp;Matthias Wieland","doi":"10.1016/j.rvsc.2024.105510","DOIUrl":"10.1016/j.rvsc.2024.105510","url":null,"abstract":"<div><div>Delayed milk ejection (DME) could compromise udder health and milking performance. The objectives of this systematic review and meta-analysis were to (i) explore the current evidence, assess the risk of bias, and identify the knowledge gaps in published studies that investigated the effect of DME on udder health and milking performance in dairy cows, and (ii) quantify the magnitude of the difference in milk yield per milking session (MY), milking duration (MD), and peak milk flow rate (PFR) between cows with and without DME. We performed the current systematic review and meta-analysis according to PRISMA 2020 statement. We tracked the pertinent peer-reviewed publications from PubMed using the following syntax (delayed milk ejection OR disturbed milk ejection OR bimodal milk flow OR bimodality) AND (bovine OR cattle OR cows), and additional relevant studies from the identified articles' reference lists. We calculated the pooled-mean difference in MY (kg), MD (seconds), and PFR (kg/min) between cows with and without DME using the random-effects models. The selection process identified 15 studies as the final number of studies qualified for review, including six interventional and nine observational studies published between 1980 and 2023 conducted in seven countries, including the USA, Germany, Italy, Switzerland, Netherlands, New Zealand, and Portugal. The results indicated that while the DME had a minimal influence on the udder health, it can reduce MY by 1.55 kg per milking session (95 % CI = −2.10, −1.00). The observed evidence here suggests that DME can negatively influence the milking profitability.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105510"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant Enterobacteriaceae from dairy cattle milk: A systematic review and meta-analysis","authors":"Dian Meididewi Nuraini ,&nbsp;Morsid Andityas ,&nbsp;Peerapol Sukon ,&nbsp;Patchara Phuektes","doi":"10.1016/j.rvsc.2024.105497","DOIUrl":"10.1016/j.rvsc.2024.105497","url":null,"abstract":"<div><div>Carbapenem-resistant <em>Enterobacteriaceae</em> (CRE) have been detected in dairy cattle milk, raising concerns about public health risks. This study aimed to assess the global prevalence of CRE in dairy cattle milk through a systematic review and meta-analysis, following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Retrieved articles from four databases were initially screened based on predefined inclusion criteria. The meta-analysis included 49 studies (2011–2024), covering 28,134 milk samples and 3462 <em>Enterobacteriaceae</em> isolates globally. Data from the full text were extracted to a Microsoft Excel spreadsheet and analysed using the ‘meta’ R package in R v.4.3.0 software for pooled prevalence and subgroup meta-analysis with a random-effects for logit transformation. Heterogeneity was assessed using Cochran's Q statistic (χ²), <em>p</em>-value and <em>I</em>^<em>2</em> statistic. Publication bias and sensitivity were evaluated using Egger's test, funnel plot, trim and fill plot, and leave-one-out test. Globally, the prevalence of CRE in dairy cattle milk was 0.73 % (95 % CI, 0.37–1.41). Subgroup meta-analysis based on continent, sample type, <em>Enterobacteriaceae</em> species, diagnostic method, antibiotic type, and interpretation guideline revealed no significant differences among the criteria within the subgroup. Although the overall pooled prevalence of CRE in dairy cattle milk is relatively low, it raises public health concern regarding raw milk consumption. This emphasizes the need for regular monitoring with in a One Health framework for CRE in the dairy industry to anticipate potential transmission between humans, animals, and the environment.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105497"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of prevalence, risk factors, and therapeutic approach for subclinical endometritis and oviductal occlusion in repeat breeder Holstein cattle","authors":"Sofía L. Villar ,&nbsp;Carlos C. Pérez-Marín ,&nbsp;Jacobo Álvarez ,&nbsp;Uxía Yáñez ,&nbsp;Juan J. Becerra ,&nbsp;Ana I. Peña ,&nbsp;Pedro G. Herradón ,&nbsp;Luis A. Quintela","doi":"10.1016/j.rvsc.2024.105511","DOIUrl":"10.1016/j.rvsc.2024.105511","url":null,"abstract":"<div><div>Due to the productive and economic consequences of Repeat Breeder (RB) syndrome, the objectives of this study were to determine the prevalence and risk factors for subclinical endometritis (SE) and oviductal occlusion (OO) in RB cows, and to make a therapeutic approach for these pathologies. In 99 RB cows, endometrial cytologies were performed to assess the presence of SE (&gt;5 % polymorphonuclear neutrophils), and the oviductal patency was checked using the phenolsulfonphthalein test. Body condition score was evaluated, and data from each animal were obtained from on-farm software (parity, calving date, artificial insemination (AI) date, number of AI, and occurrence of postpartum diseases). Cows positive to SE were assigned to one treatment protocol: a) Non-steroidal anti-inflammatory drug (NSAID), b) Prostaglandin F_2α (PGF_2α), c) NSAID+ PGF_2α. Similarly, cows with OO were assigned to one treatment: a) Therapeutic embryo, b) Artificial insemination. The prevalence for SE and OO was 22 % and 3 %, respectively. Regarding SE, the logistic regression did not show any significant difference for the risk factors evaluated. Moreover, cows that were administered the NSAID, either alone or in combination with PGF_2α, showed higher conception rates in the following AI (<em>p</em> &lt; 0.05). Due to the low prevalence of OO, it was not possible to perform the logistic regression analysis. In conclusion, neither SE nor OO seem to be the main cause of RB syndrome in the cows under study. Additionally, when SE is diagnosed as the main cause of RB syndrome, treatment with NAISDs seems to increase conception rates.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105511"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary steps for fabrication of microfluidic systems for swine sperm sorting: Materials, perfusing systems and flow","authors":"A. Lacueva-Aparicio ,&nbsp;R. Monge ,&nbsp;L. Serrano ,&nbsp;C. Malo","doi":"10.1016/j.rvsc.2024.105488","DOIUrl":"10.1016/j.rvsc.2024.105488","url":null,"abstract":"<div><div>The success rate of assisted reproductive techniques in the livestock production can be optimized by improving the quality of the semen sample by selecting only the good quality sperm from the ejaculate. Microfluidic technology has been studied for sperm sorting mainly in human ejaculates but has not been studied for boar sperm. Spermatozoa have been proven to be highly sensitive to different microplastics, but the potential toxic effects of the materials used to set up microfluidic systems have not been studied. The main goal of this study was to assess the possible toxic effect on boar sperm of materials commonly used for a microfluidic system and to evaluate the effect of different flow control systems (peristaltic pump, syringe pump and a microfluidic flow controller) at different flow rates (10 μl*min-1, 100 μl*min-1 and 1 ml*min-1) on sperm quality, as preliminary information for the development of a swine sperm sorting microfluidic system. Results showed no negative effect of the different materials at different concentrations. The control reached the highest curvilinear velocity compared to the peristaltic pump and the pressure-based flow control system. In the flow rates, 10 μm*min-1 showed the poorest results and no significant differences were observed between control and 1 mlmin-1 flow in any of the parameters. In conclusion, all materials that were studied for microfluidic fabrications were suitable for sperm sorting, any of the pumps would be suitable for sperm selection and 1 ml*min-1 flow rate would be the flow rate of choice for sperm pumping.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105488"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct amino acid substitutions in the EEV glycoprotein and DNA-dependent RNA polymerase of lumpy skin disease virus identified in wetland areas of Bangladesh","authors":"Moslema Jahan Mou ,&nbsp;S.M. Nazmul Hasan ,&nbsp;Anandha Mozumder ,&nbsp;Marjana Akter ,&nbsp;Riyan Al Islam Reshad ,&nbsp;Roni Mia ,&nbsp;Md. Salauddin ,&nbsp;M. Shaminur Rahman ,&nbsp;Md. Mahmudul Alam ,&nbsp;Sharmin Akter ,&nbsp;Sukumar Saha ,&nbsp;Tofazzal Islam ,&nbsp;Md. Golzar Hossain","doi":"10.1016/j.rvsc.2024.105508","DOIUrl":"10.1016/j.rvsc.2024.105508","url":null,"abstract":"<div><div>The recent outbreak of lumpy skin disease virus (LSDV) in the wetland areas of Bangladesh presents a significant concern for both animal health and regional biosecurity. Epidemiological investigations into nine major outbreaks in the wetland areas revealed distinctive clinical symptoms in affected cattle, including elevated body temperature, excessive salivation, and the presence of skin nodules. Histopathological examination unveiled larger nodules compared to previous outbreaks, along with signs of secondary infection. Molecular analysis confirmed the presence of LSDV in all samples, with subsequent sequencing revealing genetic similarities with virus isolates of Bangladesh, India, China, Russia, Serbia and Greece. Most importantly amino acid variations in the viral EEV glycoprotein and DNA-dependent RNA polymerase were revealed that also altered the structures of the respective proteins significantly suggesting potential implications for viral pathogenesis. Additionally, successful isolation of LSDV in Vero cells demonstrated cytopathic effects, supporting the potential for vaccine development. In conclusion, this study provides comprehensive insights into the epidemiology, genomic characters with altered predicted structures of two major viral proteins and pathogenesis of LSDV outbreaks in Bangladesh. These findings emphasize the critical need for ongoing monitoring and adaptive control strategies, including the development of effective vaccines, to mitigate the impact of LSDV in affected regions and safeguard regional livestock health.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"183 ","pages":"Article 105508"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}