Research in veterinary science最新文献

{"title":"Trichinella spp. in wolves (Canis lupus) and red foxes (Vulpes vulpes) of southern Italy","authors":"Stefano Scarcelli ,&nbsp;Francesco Buono ,&nbsp;Nicola D'Alessio ,&nbsp;Simona Rea ,&nbsp;Elisa Castaldo ,&nbsp;Antonella Pesce ,&nbsp;Francesco Locantore ,&nbsp;Alessandro Fioretti ,&nbsp;Gianluca Marucci ,&nbsp;Giovanni Sgroi ,&nbsp;David Modrý ,&nbsp;Vincenzo Veneziano","doi":"10.1016/j.rvsc.2024.105381","DOIUrl":"10.1016/j.rvsc.2024.105381","url":null,"abstract":"<div><p><em>Trichinella</em> spp. are cosmopolitan parasites that infect a wide range of hosts, with wildlife being the main reservoir of these zoonotic nematodes, especially red foxes (<em>Vulpes vulpes</em>) and wolves (<em>Canis lupus</em>) due to their apex position in the food chain in most European countries. The aim of this study is to investigate the prevalence of <em>Trichinella</em> spp. in these wild canids and their epidemiological role in the Campania region (southern Italy). From 2017 to 2023, the carcasses of red foxes (<em>n</em> = 352) and wolves (<em>n</em> = 41) were collected as part of a health surveillance plan. Muscle samples were analysed individually by artificial digestion and four (1.1%) red foxes and nine (21.9%) wolves tested positive for <em>Trichinella britovi</em>. All <em>Trichinella</em> isolates were identified as <em>T. britovi</em> by multiplex PCR. Statistically significant differences in prevalence were found by province (<em>p</em>-value = 0.05) for red foxes and sampling years (p-value = 0.01) for wolves. The prevalence was lower in red foxes than in wolves, probably due to the longer life expectancy of wolves compared to red foxes and the role of wolves as apex predators compared to red foxes as meso-carnivores. The results obtained confirm the important role that these wild canids play in the circulation of the parasite.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"179 ","pages":"Article 105381"},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of bacteremia, bacteriuria and bacteriuria-related bacteremia in dogs and cats with chronic kidney disease. A pilot study","authors":"Annamaria Uva ,&nbsp;Maria Alfonsa Cavalera ,&nbsp;Floriana Gernone ,&nbsp;Souad Nasar ,&nbsp;Paola Ghergo ,&nbsp;Marco Cordisco ,&nbsp;Marialaura Corrente ,&nbsp;Andrea Zatelli","doi":"10.1016/j.rvsc.2024.105382","DOIUrl":"10.1016/j.rvsc.2024.105382","url":null,"abstract":"<div><p>In human medicine, major infections are the most significant and critical non-cardiovascular complications in patients affected by chronic kidney disease (CKD), with bacteriuria being the primary source of bloodstream infections and its evolution toward sepsis. The availability of data on prevalence of bacteremia and its association with bacteriuria in dogs and cats with CKD is limited. The aim of this observational cross-sectional study was to determine the occurrence of bacteremia, bacteriuria, and bacteriuria-related bacteremia in dogs and cats affected by CKD. Client-owned dogs and cats with a documented history of CKD undergoing disease follow-up were enrolled. Each included animal underwent a comprehensive physical examination, clinico-pathological and microbiological analyses of blood and urine, along with molecular detection of the 16S rRNA bacterial gene in blood. Aseptically collected blood and urine were obtained through jugular venipuncture and cystocentesis, respectively. After collection, blood and urine samples underwent bacteriological culture within one hour. In the population enrolled, 2/47 dogs and 1/41 cats presented bacteriemia. Moreover, 8/47 dogs and 6/41 cats presented a positive urine culture. Additionally, in one out of the 47 dogs, the same pathogen was identified from blood and urine samples, with a final diagnosis of urosepsis. No instances of bacteriuria-related bacteriemia were observed in the cat population. In conclusion, this study shows a low prevalence of bacteremia and confirms a high prevalence of bacteriuria in companion animals affected by CKD. Moreover, a low prevalence of bacteriuria-related bacteremia was also found.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"179 ","pages":"Article 105382"},"PeriodicalIF":2.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824002492/pdfft?md5=e1617ee0340403c7958c4e394c40d04b&pid=1-s2.0-S0034528824002492-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142077251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in one‑carbon metabolism and protein synthesis signals due to methionine supplementation and lipopolysaccharide challenge in Holstein fetal liver explants","authors":"Ahmad A. Aboragah ,&nbsp;Nithat Wichasit ,&nbsp;Abdulrahman A. Alharthi ,&nbsp;Ibrahim A. Alhidary ,&nbsp;Juan J. Loor","doi":"10.1016/j.rvsc.2024.105386","DOIUrl":"10.1016/j.rvsc.2024.105386","url":null,"abstract":"<div><p>One‑carbon metabolism (OCM) fueled by methionine (Met), choline, and folic acid is key for embryo development and fetal growth. We investigated effects of lipopolysaccharide (LPS) to induce inflammation in fetal liver tissue with or without Met on components of OCM and protein synthesis activity. Fetal liver harvested at slaughter from six multiparous pregnant Holstein dairy cows (37 ± 6 kg milk/d, 100 ± 3 d gestation) were incubated (0.2 ± 0.02 g) for 4 h at 37 °C with each of the following: ideal profile of amino acids (control; Lysine:Met 2.9:1), control plus LPS (1 μg/mL), increased Met supply (Met, Lys:Met 2.5:1), and Met+LPS. Data were analyzed as a 2 × 2 factorial (PROC MIXED, SAS 9.4). Ratios of mechanistic target of rapamycin (p-mTOR:mTOR) and eukaryotic elongation factor 2 (p-eEF2:eEF2) protein were lowest (<em>P</em> &lt; 0.0 5) with LPS and highest with Met. Tissue amino acid concentrations were lowest (<em>P</em> &lt; 0.0 5) with Met regardless of LPS suggesting enhanced use via mTOR. The marked increase (<em>P</em> = 0.02) in phosphorylation of S6 ribosomal protein (p-RPS6) with LPS suggested a pro-inflammatory response that was partly alleviated with Met+LPS. No effect (<em>P</em> = 0.4 5) on methionine adenosyl transferase 1 A (MAT1A) protein abundance was detected. Activity of betaine-homocysteine <em>S</em>-methyltransferase (BHMT) was greatest with Met, but Met+LPS dampened this effect (<em>P</em> = 0.0 5). Overall, fetal liver responds to inflammatory challenges and Met supply. The latter can stimulate protein synthesis via mTOR and alter some OCM reactions while having a modest anti-inflammatory effect.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"178 ","pages":"Article 105386"},"PeriodicalIF":2.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolites and physical scores as possible predictors for postpartum culling in dairy cows","authors":"Kyoko Chisato ,&nbsp;Takerou Yamazaki ,&nbsp;Shuji Kayasaki ,&nbsp;Rika Fukumori ,&nbsp;Hidetoshi Higuchi ,&nbsp;Kohei Makita ,&nbsp;Shin Oikawa","doi":"10.1016/j.rvsc.2024.105387","DOIUrl":"10.1016/j.rvsc.2024.105387","url":null,"abstract":"<div><p>The purpose of the study was to explore the associations of serum non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations with the body condition score (BCS) and rumen fill score (RFS) in order to predict the risk of postpartum culling, and to further investigate effective monitoring stages during the dry period. From October 2012 to March 2014, clinically healthy Holstein heifers and cows were sampled once before calving, and the occurrence of culling within 60 days in milk (DIM) was investigated. The discriminatory ability of each parameter was evaluated using receiver operating characteristic (ROC) analysis. Of the 720 cows sampled between 14 and 2 days before the actual day of calving in the study, 42 cull cows (mean DIM ± SE: 22.0 ± 2.6) were confirmed. The areas under the curve (AUC) of the ROC for predicting culling using serum NEFA concentrations were 0.6 and 0.7 at 14 to 2 and 7 to 2 days before calving, respectively. The AUC for the RFS was 0.7 for both periods, indicating the same diagnostic level as the serum NEFA concentration. Both the serum NEFA concentration and RFS were possible predictors in cows with ≥ 2 parities, but not in cows with 0–1 parity sampled even at 7 to 2 days before calving. The serum BHBA concentration and BCS were not suitable predictors of culling for any period or parity. These results indicate that RFS has a discriminatory ability comparable to the serum NEFA concentration for predicting culling within 60 DIM.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"179 ","pages":"Article 105387"},"PeriodicalIF":2.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142088213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of nasopharyngeal microbiota revealing microbial disturbance associated with ovine respiratory complex","authors":"Hui Jiang ,&nbsp;Luo Yang ,&nbsp;Shiyu Duan ,&nbsp;Rongzheng Wu ,&nbsp;Mengyue Li ,&nbsp;Bo Liu ,&nbsp;Yiping Zhu ,&nbsp;Jing Li","doi":"10.1016/j.rvsc.2024.105383","DOIUrl":"10.1016/j.rvsc.2024.105383","url":null,"abstract":"<div><p>The ovine respiratory complex (ORC) is a complex respiratory disease process in ovine causing a significant health concern with substantial morbidity and mortality. It poses a significant threat of impacting animal health, leading to severe health consequences and considerable economic loss. Research on the upper respiratory tract (URT) microbiota is critical for offering insights into pathogenesis, prevention, and treatment strategies of ORC. The goal of this study is to compare the nasopharyngeal microbiota of clinically healthy Hu sheep (Group H) and sheep with ORC (Group P). The 16S rDNA gene amplicon high-throughput sequencing technique was applied to identify the microbial composition in the nasopharyngeal samples. The results showed that there was no significant difference in the microbiota diversity and richness between ORC and healthy sheep. However, there were significant differences in microbial composition, such as the relative abundance of Enterobacteriaceae, Moraxellaceae, Pasteurellaceae, and Streptococcaceae between the two groups. The abundance of aerobes in sheep with ORC increased significantly, while the abundance of anaerobes and facultative anaerobes decreased significantly. There were also differences in the taxa phenotypes associated with biofilm forming, mobile element-containing, oxidative stress-tolerance, and potential pathogens between the two groups. Our study showed the nasopharyngeal microbiota composition and its associated shifts between clinically healthy sheep and ORC sheep in China. These findings suggest that shifts in the nasopharyngeal microbiota could be a contributing factor to the pathogenesis of ORC, offering a potential avenue for the development of targeted interventions and treatments for this condition in sheep.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"179 ","pages":"Article 105383"},"PeriodicalIF":2.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142088212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Position and frequency of lateral canals in carnassial teeth of dogs","authors":"Faruk Tandir ,&nbsp;Rizah Avdić ,&nbsp;Nejra Dučić ,&nbsp;Aida Džanković ,&nbsp;Redžep Tandir ,&nbsp;Ermin Šaljić ,&nbsp;Anel Vejzović ,&nbsp;Nedžad Hadžiomerović","doi":"10.1016/j.rvsc.2024.105384","DOIUrl":"10.1016/j.rvsc.2024.105384","url":null,"abstract":"<div><p>Carnassial teeth, as one of the most important teeth in dogs, are commonly affected by different types of injuries and pathological disorders, which require endodontic treatment. Lateral canals, which leave the main root canal, and apical deltas are present in the root canal system of dogs. These canals represent small branches that leave the main root canal at a sharp angle and terminate in a region of the periodontal ligament on the external root surface. In this study, 200 teeth were included, of which 100 were superior fourth premolars and 100 were inferior first molars. Lateral canals were present in a total of 30 roots of superior fourth premolar teeth, whereas in inferior first molars, these canals were found in 3% of roots. In superior fourth premolars, lateral canals were mostly present in the apical third, 86%, while 14% were present in the middle third of the root. In inferior first molars, lateral canals were found in 3% of the roots, 2% in the apical third and 1% in the middle third of the roots. The knowledge of the complex root canal morphology is essential for any endodontic treatment. Due to the presence and frequency of lateral canals, it is imperative to perform more thorough cleaning and instrumentation while performing endodontic treatment on superior fourth premolars compared to inferior first molars.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"179 ","pages":"Article 105384"},"PeriodicalIF":2.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Existence of Blastocystis infection in bar–headed goose (Anser indicus)","authors":"Nian-Yu Xue ,&nbsp;Si-Yuan Qin ,&nbsp;Ya Qin ,&nbsp;Hai-Tao Wang ,&nbsp;Qing-Yu Hou ,&nbsp;Xing Yang ,&nbsp;Jing Jiang ,&nbsp;Hong-Bo Ni","doi":"10.1016/j.rvsc.2024.105380","DOIUrl":"10.1016/j.rvsc.2024.105380","url":null,"abstract":"<div><p><em>Blastocystis</em> is a common intestinal protist in humans and animals. Currently, <em>Blastocystis</em> infection in bar-headed geese is unknown. To understand the prevalence and distribution of <em>Blastocystis</em> subtypes in bar–headed geese, fecal samples were collected from 358 bar–headed geese in three regions. The total infection rate of <em>Blastocystis</em> in bar–headed in China was 5.9% (21/358), with 5.0% (6/120) in Aba (Ngawa) Tibetan and Qiang Autonomous Prefect, Sichuan province, 11.8% (14/119) in Maqu county, Gansu province, and 0.8% (1/119) in Caohai, Wei ning county, Guizhou province. The differences in prevalence rates by region were statistically significant. Sequences analyses showed that one known subtype (ST7, <em>n</em> = 16) and one potentially novel ST (<em>n</em> = 5) in bar–headed geese were detected in this study. This is the first report on the prevalence and subtype distribution of <em>Blastocystis</em> in bar–headed geese, which will improve our understanding of the epidemiology and public health implications of <em>Blastocystis</em> infection in wild migratory birds.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"178 ","pages":"Article 105380"},"PeriodicalIF":2.2,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The need for assisted ventilation corroborates the effectiveness of antenatal corticosteroid therapy in preventing premature lamb mortality","authors":"Fernanda Machado Regazzi,&nbsp;Beatriz de Melo Justo,&nbsp;Ana Beatriz Giraldi Vidal,&nbsp;Maíra Morales Brito,&nbsp;Renata Azevedo de Abreu,&nbsp;Leticia Lima de Almeida,&nbsp;Camila Infantosi Vannucchi","doi":"10.1016/j.rvsc.2024.105379","DOIUrl":"10.1016/j.rvsc.2024.105379","url":null,"abstract":"<div><p>In premature births, deficiency and/or inactivation of surfactant and incomplete development of lung occur, leading to pulmonary complications and greater need for ventilatory interventions. Prenatal corticosteroid therapy is used to improve neonatal lung function and, thus, may reduce mortality and lower incidence and severity of lung injury. Therefore, this study aimed to assess the need for ventilatory support in preterm lambs subjected or not to prenatal betamethasone treatment, and to evaluate the effectiveness on neonatal survival. Lambing was induced and 13 premature lambs were assigned to Corticosteroid Group (<em>n</em> = 8; lambs from ewes subjected previously to 0.5 mg/kg betamethasone, IM, at 133 days of pregnancy) and Control Group (<em>n</em> = 5; non-treated lambs). Lambs were evaluated for vitality, neurologic reflexes, vital functions and birth weight. Three ventilatory modalities were preconized for critical lambs, according to specific criteria: mask oxygen therapy, self-inflating bag with tracheal tube and mechanical ventilation. Non-treated lambs had lower vitality score, muscle tonus and respiratory rate compared to Corticosteroid Group. Ventilatory support was needed for 3 Control lambs and only 1 Corticosteroid neonate. Corticosteroid lamb required significant less time-frame between birth and onset of ventilatory assistance and remained under ventilation for a shorter time. Percentage of ventilated non-treated lambs correlated negatively with birth weight, muscle tone, heart and respiratory rate. In conclusion, antenatal betamethasone treatment reduces the need for ventilatory assistance in premature lambs. Additionally, mortality is low when a protocol for inducing pulmonary maturity (maternal corticosteroid therapy) and/or ventilatory interventions are employed, ensuring the survival of premature lambs.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"178 ","pages":"Article 105379"},"PeriodicalIF":2.2,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does the proximal screw type affect stress and strain in pastern arthrodesis with locking plate in horses?","authors":"Anderson Fernando de Souza ,&nbsp;César Augusto Martins Pereira ,&nbsp;Anton Fürst ,&nbsp;Jan Michael Kümmerle ,&nbsp;André Luis do Valle De Zoppa","doi":"10.1016/j.rvsc.2024.105378","DOIUrl":"10.1016/j.rvsc.2024.105378","url":null,"abstract":"<div><p>The implantation of unicortical cortex screws in the proximal hole of locking compression plates (LCP) has been recommended for proximal interphalangeal (PIP) arthrodesis in horses to prevent fractures resulting from stress risers in the proximal phalanx (P1). However, this cortex screw fixation technique may limit efficient dorsal compression of the PIP joint by the plate, potentially affecting the stability of the construct. In this study, we aimed to measure stress and strain in P1 and the plate using an ex vivo model of PIP arthrodesis in horses. We employed various implantation methods and proximal screw types in conjunction with two 5.5 mm transarticular cortex screws. Ten pairs of equine forelimbs were divided into four groups based on proximal screw placement: GUC (unicortically placed cortex screw), GBC (bicortically placed cortex screw), GUL (unicortically placed locking screw), and GBL (bicortically placed locking screw). We calculated the magnitude and direction of strain, strain ratio, and stress using strain gauges during an axial compression mechanical testing. The palmar surface of P1 exhibited higher stress and strains than the dorsal surface, with the plate part located at the articular level suffered more stress than the proximal part. Both the implantation method and proximal screw type significantly influenced the analyzed parameters. The GUC promoted greater changes in strain direction in the proximal portion of the P1. Bicortical placement of a cortex screw appears to be the most suitable option for filling the proximal hole of the LCP, because it allows effective dynamic compression via the plate and prevents abrupt shifts in the direction of the forces acting on the proximal part of P1 during loading.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"178 ","pages":"Article 105378"},"PeriodicalIF":2.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141952779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an artificial intelligence-based algorithm for predicting the severity of myxomatous mitral valve disease from thoracic radiographs by using two grading systems","authors":"Carlotta Valente ,&nbsp;Marek Wodzinski ,&nbsp;Carlo Guglielmini ,&nbsp;Helen Poser ,&nbsp;David Chiavegato ,&nbsp;Alessandro Zotti ,&nbsp;Roberto Venturini ,&nbsp;Tommaso Banzato","doi":"10.1016/j.rvsc.2024.105377","DOIUrl":"10.1016/j.rvsc.2024.105377","url":null,"abstract":"<div><p>A heart-convolutional neural network (heart-CNN) was designed and tested for the automatic classification of chest radiographs in dogs affected by myxomatous mitral valve disease (MMVD) at different stages of disease severity. A retrospective and multicenter study was conducted. Lateral radiographs of dogs with concomitant X-ray and echocardiographic examination were selected from the internal databases of two institutions. Dogs were classified as healthy, B1, B2, C and D, based on American College of Veterinary Internal Medicine (ACVIM) guidelines, and as healthy, mild, moderate, severe and late stage, based on Mitral INsufficiency Echocardiographic (MINE) score. Heart-CNN performance was evaluated using confusion matrices, receiver operating characteristic curves, and t-SNE and UMAP analysis. The area under the curve (AUC) was 0.88, 0.88, 0.79, 0.89 and 0.84 for healthy and ACVIM stage B1, B2, C and D, respectively. According to the MINE score, the AUC was 0.90, 0.86, 0.71, 0.82 and 0.82 for healthy, mild, moderate, severe and late stage, respectively. The developed algorithm showed good accuracy in predicting MMVD stages based on both classification systems, proving a potentially useful tool in the early diagnosis of canine MMVD.</p></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"178 ","pages":"Article 105377"},"PeriodicalIF":2.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0034528824002443/pdfft?md5=17c3f0a6e955c2fe58614582a00e2f10&pid=1-s2.0-S0034528824002443-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141952780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}