Research in veterinary science最新文献

{"title":"Evaluation of the impact of acepromazine on tissue oxygenation in horses sedated with detomidine","authors":"Amanda L. James ,&nbsp;Enzo Vettorato ,&nbsp;Raiane A. Moura ,&nbsp;Tatiana Moreira Batista P.R. Azevedo ,&nbsp;Martha F. Mallicote ,&nbsp;Ludovica Chiavaccini","doi":"10.1016/j.rvsc.2025.106019","DOIUrl":"10.1016/j.rvsc.2025.106019","url":null,"abstract":"<div><div>Sedation with α₂-agonists can impair microcirculation and oxygen delivery. This prospective randomized crossover study investigated whether administering acepromazine maleate before detomidine hydrochloride maintains higher peripheral tissue oxygen saturation (StO₂) in sedated horses. Eight healthy adult horses randomly received intravenous detomidine (10 μg/kg) either alone (D) or in combination with acepromazine (20 μg/kg; DA), with a one-week washout. Hemoglobin concentration and central venous oxygen saturation (ScvO₂) were measured at baseline. Tissue oxygen saturation, tissue hemoglobin index (THI), sedation depth, heart rate (HR), respiratory rate (<em>f</em>R), mean arterial pressure (MAP), gastrointestinal motility, and serum lactate concentrations were recorded at baseline, 5, 15, 30, 60, and 120 min after injection. Mixed-effects linear models were used to assess time and treatment effects, and correlations were analyzed using the Bland-Altman Repeated Measures correlation. Across both treatment groups, StO₂ (<em>p</em> &lt; 0.011), HR (<em>p</em> ≤ 0.007), <em>f</em>R (<em>p</em> &lt; 0.0001), MAP (<em>p</em> ≤ 0.024), and gastrointestinal motility (p &lt; 0.0001) declined significantly over time with no significant difference between groups. A negative correlation between StO₂ and serum lactate was found (<em>r</em> = −0.37, <em>p</em> = 0.0004); however, no correlation with ScvO₂ was observed. Sedation scores increased from 5 to 60 min (<em>p</em> &lt; 0.001), ranging between 5 and 8 regardless of group allocation and were overall higher in the DA group (<em>p</em> = 0.02). These findings suggest that acepromazine enhances the sedative effect of detomidine but may not fully counteract its adverse impact on peripheral tissue oxygenation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106019"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsatellite-based genetic diversity and population structure of local goat breeds in Cyprus","authors":"Raziye Işık Kalpar ,&nbsp;Emel Özkan Ünal ,&nbsp;Abdullah Taner Önaldi ,&nbsp;Nazan Koluman ,&nbsp;Gönen Vurana ,&nbsp;Ersin Gözenler ,&nbsp;Bulut Olgu ,&nbsp;Ayşen Bulancak ,&nbsp;Ahmet Ince","doi":"10.1016/j.rvsc.2025.106033","DOIUrl":"10.1016/j.rvsc.2025.106033","url":null,"abstract":"<div><div>This study investigated the genetic diversity of three economically important goat breeds, Damascus, Pisuri, and Mahari, from Cyprus, utilizing 20 heterologous microsatellite loci. A total of 200 alleles were detected, averaging 10 alleles per locus. Damascus showed the highest genetic diversity (Na = 8.5), followed by Pisuri and Mahari (Na = 8.3). The effective number of alleles ranged from 2.589 (INRA005) to 8.891 (HSC), and Polymorphic Information Content (PIC) values (0.55–0.86) confirmed high polymorphism across loci. Observed (H_O) and expected (H_E) heterozygosities ranged from 0.4118 to 0.9574 and 0.4890 to 0.9016, respectively, with Pisuri displaying the highest genetic diversity. The mean inbreeding coefficient (F_IS = 0.061) indicated moderate inbreeding. Genetic differentiation was low to moderate (F_ST = 0.0068–0.1298), with Damascus being the most distinct population. STRUCTURE and factorial analyses supported the presence of three genetic clusters, clearly separating Damascus from Pisuri and Mahari. Despite minor deviations from Hardy–Weinberg equilibrium, the overall results revealed limited differentiation and high genetic diversity, particularly in Damascus. These findings provide valuable insights into the genetic composition of Cyprus goat breeds and highlight their importance for conservation and selective breeding strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106033"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genetic analysis of the causes of lamb mortality determined by an on-farm postmortem procedure","authors":"K. Hay ,&nbsp;N. Lambe ,&nbsp;J. Roden ,&nbsp;S. Jarvis ,&nbsp;C.M. Dwyer","doi":"10.1016/j.rvsc.2025.106032","DOIUrl":"10.1016/j.rvsc.2025.106032","url":null,"abstract":"<div><div>Lamb mortality is a major challenge in sheep production with significant implications for animal welfare and farm profitability. This study investigated the causes of lamb mortality within the first three days of life in a lowland outdoor lambing flock in the Scottish Borders, UK, over three lambing seasons (2021−2023). Dead lambs were collected from the pastures during checking rounds three times a day, and simple on-farm postmortems were conducted once a day. A total of 468 lamb postmortem examinations were conducted to classify the most likely cause of death as dystocia, starvation/mismothering/exposure (SME), stillbirth, other, or unknown causes. Overall mortality to three days of age was 12 %, with SME (28 %) and stillbirth (27 %) the most common causes, followed by dystocia (13 %). Single and triplet born lambs (<em>p</em> &lt; 0.01) were most likely to die from dystocia compared to other litter sizes and the risk of death from SME increased with litter size (<em>p</em> &lt; 0.01). Direct lamb heritability estimates for death by dystocia and stillbirth were moderate (0.31 and 0.27, respectively), indicating potential for improvement via genetic selection. However, heritability for SME was not significant, highlighting the increased influence of environmental factors for this cause of lamb death. The findings demonstrate that the use of simple postmortems could be used to increase the accuracy of selection for lamb survival in breeding programmes, through the integration of breeding values for specific causes of death. This would be most applicable in well-recorded nucleus flocks that are well connected to the rest of the breeding programme.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106032"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the usual signs: Pseudo-pericarditis as an under-appreciated outcome of tropical theileriosis","authors":"Vikrant Sudan ,&nbsp;Priyanka Syal ,&nbsp;Jasleen Kaur","doi":"10.1016/j.rvsc.2025.106029","DOIUrl":"10.1016/j.rvsc.2025.106029","url":null,"abstract":"<div><div>Pseudopericarditis is an often-overlooked clinical manifestation of tropical theileriosis, with only a few sporadic reports describing its occurrence. The present note documents clinical cases of tropical theileriosis exhibiting characteristic signs of pseudopericarditis, including jugular vein distension and brisket oedema. The proposed pathogenesis, supported by a schematic illustration, suggests that enlarged mediastinal lymph nodes exert pressure on major intrathoracic vessels, thereby impairing venous return and producing the clinical signs observed. Additionally, the roles of oncotic, osmotic, and hydrostatic pressure imbalances in the pathobiology of pseudopericarditis have been emphasised. Field veterinarians should therefore consider tropical theileriosis as a differential diagnosis in animals presenting with brisket oedema and/or jugular vein distension.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106029"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive components of Azadirachta indica (neem) leaf extract enhance immunity, health, and production parameters in broilers","authors":"Md. Shafiqul Islam,&nbsp;Md. Abu Rayhan Parvez,&nbsp;Saiful Islam,&nbsp;Md. Tanvir Hasan,&nbsp;Md. Ashraful Alam,&nbsp;Md. Najibul Hoque","doi":"10.1016/j.rvsc.2025.106040","DOIUrl":"10.1016/j.rvsc.2025.106040","url":null,"abstract":"<div><div>This study investigated the efficacy of a chemically characterized neem (<em>Azadirachta indica</em>) leaf extract as a viable alternative to antibiotics for sustainable broiler production. Comprehensive phytochemical analysis (TLC, HPLC, GC–MS, NMR) identified and quantified key bioactive compounds-azadirachtin, nimbin, nimbolide, gedunin, and salannin-with the ethanolic extract yielding a higher concentration of these limonoids. The comparative efficacy of this standardized neem preparation was evaluated against danofloxacin (antibiotic) and butaphosphan (metabolic booster) in a 28-day trial with one hundred and fifty Lohman meat chicks (10 birds/pen, 3 pens/treatment). The results demonstrated that neem supplementation significantly improved broiler performance. Birds in the neem groups, particularly the ethanolic group, achieved significantly higher final body weight and better feed conversion ratio, performing comparably to the antibiotic and booster groups. Immunologically, neem extracts significantly increased lymphocyte counts and reduced the heterophil-to-lymphocyte ratio, confirming an immunostimulatory and anti-stress effect. Serum biochemistry revealed significantly lower levels of liver enzymes (AST and ALT), indicating a hepatoprotective benefit. Histopathological examination confirmed healthier intestinal morphology, with quantifiably increased villus height and a superior villus-height-to-crypt-depth ratio in the duodenum and more caecal folds. Additionally, carcass quality and dressing percentage were significantly higher in neem-treated birds. The study concludes that the synergistic action of the identified bioactive compounds in neem leaf extract is responsible for its multifaceted benefits, making it a promising, natural, and cost-effective phytogenic alternative to in-feed antibiotics for enhancing productivity and ensuring safe broiler meat.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106040"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femorotibial joint rotation in intact joints and following partial medial collateral ligament transection, tibial plateau leveling osteotomy, and cranial cruciate ligament transection – A limb press study","authors":"M.L. Holmbjerg ,&nbsp;C.K. Tvedsborg ,&nbsp;M.B.M. Nielsen,&nbsp;J.E. Miles","doi":"10.1016/j.rvsc.2025.106026","DOIUrl":"10.1016/j.rvsc.2025.106026","url":null,"abstract":"<div><div>Despite widespread use of tibial plateau leveling osteotomy (TPLO), the influence of partial medial collateral ligament transection (pMCLX) and physiologic loading on rotational stability remains unclear. This cadaveric study quantified internal and external tibial rotation in canine stifles under simulated weightbearing conditions. Ten unpaired pelvic limbs were mounted in a custom limb press applying axial loads equivalent to 30 % body weight with the stifle fixed at 135°. Quadriceps tension was simulated using a load cell and turnbuckle, and controlled internal and external torques (0.007 Nm/kg) were applied twice per condition. Joints were tested sequentially: intact, after pMCLX at the level of the planned TPLO osteotomy, following TPLO (target tibial plateau angle 5°), and after cranial cruciate ligament transection (CCLX). Internal rotation changed minimally after pMCLX (<em>P</em> = 1.0) but increased significantly following TPLO (<em>P</em> = 0.001) and CCLX (<em>P</em> = 0.002), with limited changes following CCLX (<em>P</em> = 1.0). External rotation increased significantly only between TPLO and CCLX (<em>P</em> = 0.02). These findings indicate that pMCLX does not contribute to significant rotational laxity, while TPLO increases internal rotation independently of CCL integrity. The stabilizing effects of quadriceps tension and axial loading highlight the importance of physiologic constructs in ex vivo models. Overall, these results challenge concerns about iatrogenic instability from pMCLX and support current TPLO positioning strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106026"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical evaluation of a cemented femoral stem for canine total hip replacement across two breeds","authors":"K.S. Kamalesh Kumar ,&nbsp;Rohit Kumar ,&nbsp;T. Sai Kumar ,&nbsp;Aman Kumar Tiwari ,&nbsp;Abhishek C. Saxena ,&nbsp;O.R. Vinodh Kumar ,&nbsp;Bhanu Pratap Singh ,&nbsp;Abhijit Pawde ,&nbsp;Amarpal","doi":"10.1016/j.rvsc.2026.106057","DOIUrl":"10.1016/j.rvsc.2026.106057","url":null,"abstract":"<div><div>Cemented total hip replacement (THR) remains an established procedure for restoring hip joint function in dogs with degenerative or traumatic coxofemoral disease; however, limited evidence exists regarding the mechanical integrity and load-transfer characteristics of cemented femoral stem constructs within native canine femora. This study critically evaluated the biomechanical performance of a novel modular cemented femoral stem using cadaveric femora from German Shepherd Dogs (GSDs) and Labrador Retrievers (LABs), emphasizing axial, bending, and torsional behaviors in correlation with radiographic and morphometric parameters. Eighteen pairs of adult canine femora were harvested postmortem, with one side implanted following standardized second-generation cementation protocols and the contralateral side retained intact. Following radiographic and CT-based templating, specimens underwent displacement-controlled axial compression, three-point bending, and torsion tests. Cemented implantation significantly reduced ultimate compressive, bending, and torsional strengths compared to intact femora (<em>p</em> &lt; 0.05), though stiffness remained unaffected, suggesting preservation of elastic response despite altered failure thresholds. GSD femora consistently exhibited greater load-bearing capacity, yield strength, and energy absorption than LABs, reflecting breed-specific variations in cortical geometry and canal morphology. Radiographs confirmed precise stem alignment, uniform cement mantles averaging 5 mm, and consistent canal fill exceeding 60 %, indicative of optimized cementation. Failure patterns transitioned from brittle cortical fractures in intact specimens to ductile interfacial shear at the bone-cement interface in implanted constructs, demonstrating efficient load redistribution through the composite system. Collectively, these findings highlight the mechanical reliability and breed-dependent adaptability of the cemented THR system, supporting its translational potential for achieving durable fixation and functional load transfer in canine clinical applications while offering valuable insights relevant to comparative orthopedic biomechanics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"201 ","pages":"Article 106057"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escherichia coli strains from wild birds in Türkiye: Antibiotic resistance, virulence factors and phylogroups","authors":"Doğancan Yarım,&nbsp;Emre Karakaya,&nbsp;Fuat Aydın,&nbsp;Seçil Abay","doi":"10.1016/j.rvsc.2025.106045","DOIUrl":"10.1016/j.rvsc.2025.106045","url":null,"abstract":"<div><div>This study aimed to isolate and identify <em>Escherichia coli</em> (<em>E. coli</em>) from the feces of wild bird species and to determine antibiotic susceptibilities, phylogroups, and virulence genes in the isolates obtained.</div><div>For this purpose, a total of 575 fecal samples from 100 Eurasian Tree Sparrow, 250 Eurasian Jackdaw, 156 Eurasian Magpie, 4 Short-toed Snake-Eagle, 60 Rock dove, and 5 Long-legged Buzzard were used. <em>E. coli</em> isolation was performed using direct inoculation on MacConkey agar. The isolates were identified through phenotypic tests, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS), and Polymerase Chain Reaction (PCR). The susceptibility of the isolates to nine antibiotics was determined by the disk diffusion method. Phylogroups and the presence of virulence genes were determined by Clermont typing and the multiplex PCR (mPCR) methods, respectively. Among 575 feces samples, <em>E. coli</em> was isolated from 37 (6.43 %). The highest antibiotic resistance was found in ampicillin, azithromycin, and trimethoprim-sulfamethoxazole at 35.1 %. Moreover, 43.2 % of the <em>E. coli</em> isolates were detected as Multidrug-Resistant (MDR), and these isolates exhibited 13 different MDR patterns by demonstrating resistance to three to eight various antibiotic classes. While the most common phylogroup detected among isolates was A (18.9 %), none belonged to phylogroups C and F. 72.9 % had at least one virulence gene. The most frequently detected virulence gene was <em>stx1</em> + <em>stx2</em> (32.4 %), and all isolates were negative for <em>bfpA</em> and <em>lt</em> genes.</div><div>In conclusion, considering the role of birds of prey in the ecological balance, the data obtained in this study suggest that wild birds may contribute to the spread of pathogenic and antibiotic-resistant <em>E. coli</em> strains globally.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106045"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of endoparasite infections in the European hedgehog (Erinaceus europaeus) and association with rehabilitation outcome in wildlife centres in Northwest France","authors":"Paula Yagüez I. López-Jurado ,&nbsp;Katie Beckmann ,&nbsp;Rob Kelly","doi":"10.1016/j.rvsc.2025.106034","DOIUrl":"10.1016/j.rvsc.2025.106034","url":null,"abstract":"<div><div>The European hedgehog (<em>Erinaceus europaeus</em>) is prevalent across Western Europe, with many individuals admitted to wildlife rehabilitation centres. Parasitic infections, particularly helminth parasites, may threaten rehabilitation success. This study investigated respiratory and gastrointestinal endoparasite prevalence, risk factors, and associations with survival in 300 hedgehogs admitted to four rehabilitation centres in northwestern France (May 2023–May 2024). Faecal samples were analysed using either the modified McMaster technique (Oise) or direct smear (Seine-Maritime, Loir-et-Cher, Essonne). Overall, 58.3 % of hedgehogs carried at least one endoparasite. The most common were <em>Capillaria</em> species group (43.6 %) and <em>Crenosoma striatum</em> (25.7 %), followed by <em>Brachylaemus erinacei</em> (11 %) and <em>Cycloisospora</em> species group (7.3 %). Co-infection was common; 35 % of infected individuals harboured both <em>Capillaria</em> spp. and <em>C. striatum</em>. Younger hedgehogs showed lower prevalence but higher parasite burdens, suggesting reduced resistance. <em>Capillaria</em> spp. prevalence peaked in winter across all age groups. Multivariable analysis identified age, weight, season, and admission reason as significant risk factors for parasitism. Importantly, detection of <em>C. striatum</em> significantly increased mortality risk during rehabilitation (OR = 3.44, <em>p</em> = 0.0002). These findings highlight the need for targeted parasite screening and treatment protocols for specific risk groups, for example for juveniles and individuals with clinical signs. Coprological analysis offers a practical, non-invasive tool for parasite detection in resource-limited rehabilitation settings. This is the first study to report endoparasite prevalence in live hedgehogs in France using coprological methods and to demonstrate a statistical association between <em>C. striatum</em> infection and mortality in rehabilitation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106034"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145766055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of a novel porcine reproductive and respiratory syndrome virus strain inducing biphasic temperature fluctuations in Tianjin, northern China","authors":"Jia Wang ,&nbsp;Yi-Han Lu ,&nbsp;Bo-Han ,&nbsp;Hai-Tao Yang ,&nbsp;Xin-lei Li ,&nbsp;Wen Sun ,&nbsp;Shi-Qin Zhang ,&nbsp;Zhuo-Ran Miao ,&nbsp;Xue-Jiao Cheng ,&nbsp;Chun-Xue You ,&nbsp;Ying-Feng Sun","doi":"10.1016/j.rvsc.2025.106037","DOIUrl":"10.1016/j.rvsc.2025.106037","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome virus (PRRSV) has imposed significant economic burdens on the global swine industry. The majority of PRRSV strains can induce high fever at various stages of pig development. However, the PRRSV strain that triggers the biphasic temperature phenomenon remains relatively rare. In this study, a novel PRRSV variant, designated SJ0107, was isolated from local pig farms in Tianjin, Northern China, in 2024. Recombination analysis reveals that SJ0107 is a recombinant PRRSV strain resulting from the interaction between lineage 1.8 and lineage 8.3, with four potential recombination breakpoints located in Nsp2 (nt 766/1997), Nsp4 (nt 5413), and Nsp9 (nt 7750). Animal challenge experiments conducted on four-week-old piglets demonstrated that SJ0107 can induce rare initial hypothermia symptoms within 5 days post-inoculation (dpi). Subsequently, the body temperature returned to normal, and high-grade fever symptoms emerged at 11 dpi, lasting for one week before normalizing again. Meanwhile, severe histopathological lung lesions were also detected in the pathological sections. In conclusion, the findings reveal that the SJ0107 strain undergoes a dual-phase temperature alteration, which not only complicates traditional diagnostic criteria reliant on persistent fever but also heightens the risk of misdiagnosis in the initial phases of infection. These insights underscore the necessity of revising surveillance protocols for emerging PRRSV variants that exhibit atypical pathogenic characteristics.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"200 ","pages":"Article 106037"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}