Research in veterinary science最新文献

{"title":"Elevated levels of serum hepcidin isomers in dogs with portosystemic shunt","authors":"Zsuzsanna Vizi ,&nbsp;Katalin Lányi ,&nbsp;David Hotchkiss ,&nbsp;Ágnes Andrea Sterczer","doi":"10.1016/j.rvsc.2025.105862","DOIUrl":"10.1016/j.rvsc.2025.105862","url":null,"abstract":"<div><div>Microcytosis and iron sequestration are commonly observed abnormalities in dogs with portosystemic shunt (PSS). The role of hepcidin – the main hormone regulating iron homeostasis – in this condition is still unclear. Our study is intended to determine the serum concentrations of known hepcidin isomers in canine patients with portosystemic shunt, compared to a healthy control group.</div><div>Routine haematological and biochemical examinations were performed for 10 dogs diagnosed with PSS and for 18 healthy controls. And the serum concentrations of hepcidin-α and hepcidin-β isomers were determined by liquid chromatography-tandem mass spectrometry (LC/MS-MS) method.</div><div>In this study, both serum hepcidin isomer concentrations were significantly (<em>p</em> &lt; 0,05) higher in the PSS group compared to healthy dogs (hepcidin-α: 46.1 vs. 23.1 ng·mL⁻¹, <em>p</em> = 0.014, hepcidin-β: 76.6 vs 48.4 ng·mL⁻¹, <em>p</em> = 0.027). In dogs with PSS, there was a significant negative correlation between iron and hepcidin isomer concentrations (hepcidin-α: rho: −0.81, <em>p</em> = 0.007, hepcidin-β rho: −0.84, <em>p</em> = 0.0004).</div><div>Our results suggest that elevated serum hepcidin concentration plays an important role in the development of microcytosis and iron sequestration observed in patients with PSS; however, further studies needed in large cohort of patients. Serum hepcidin could be used as a potential biomarker in patients with PSS-associated anaemia.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105862"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of treatment and short-term follow-up of a European mink (Mustela lutreola) with leishmaniosis in Spain","authors":"José Villora ,&nbsp;Rafael Guerra ,&nbsp;Jacobo Giner ,&nbsp;Antonio Fernández ,&nbsp;María-Magdalena Alcover ,&nbsp;Álex Gómez ,&nbsp;Xavier Roca-Geronès ,&nbsp;Cristina Riera ,&nbsp;María del Carmen Aranda ,&nbsp;Roser Fisa ,&nbsp;Sergio Villanueva-Saz ,&nbsp;Diana Marteles-Aragüés","doi":"10.1016/j.rvsc.2025.105861","DOIUrl":"10.1016/j.rvsc.2025.105861","url":null,"abstract":"<div><div><em>Leishmania infantum</em> causes disease endemic in the Mediterranean Basin that affects humans and various mammals, including mustelids. Clinical leishmaniosis has been documented in domestic ferrets (<em>Mustela putorius furo</em>) and in a captive Eurasian otter (<em>Lutra lutra</em>). The European mink (<em>Mustela lutreola</em>), one of the world's most endangered mammals, is experiencing severe population declines linked to habitat degradation, climate change, the introduction of non-native species, and diseases. This report presents the first documented case of clinical leishmaniosis in a captive European mink, detailing its clinical presentation, anti-<em>Leishmania</em> therapy, and follow-up. The affected animal showed weight loss, pyogranulomatous blepharitis, and enlarged popliteal lymph nodes. Haematological and biochemical analyses revealed anaemia, hypergammaglobulinaemia, and elevated renal parameters. Diagnosis was supported by clinical signs, pathological findings, moderate anti-<em>Leishmania</em> antibody levels, and cytological detection of amastigotes in skin and lymph node samples, further confirmed by parasite culture. An initial oral regimen of miltefosine and allopurinol produced limited clinical improvement, likely due to inadequate drug intake. Subsequently, treatment with meglumine antimoniate and allopurinol resulted in a marked clinical response and resolution of the main signs. These findings underscore the need for screening for <em>L.</em> <em>infantum</em> and prompt therapeutic intervention to protect <em>Mustela lutreola</em> populations.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105861"},"PeriodicalIF":1.8,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144887312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of long-term captivity on Saiga Antelope calcaneus mechanical characteristics by three-dimensional finite element simulation","authors":"Zixian Liu ,&nbsp;Xing Gao ,&nbsp;Fengqi Wan ,&nbsp;Xinping Ma ,&nbsp;Mingcong Ding ,&nbsp;Libaihe Jing","doi":"10.1016/j.rvsc.2025.105858","DOIUrl":"10.1016/j.rvsc.2025.105858","url":null,"abstract":"<div><h3>Background</h3><div>This study investigates the mechanical properties of the calcaneus bone in saiga antelope (<em>Saiga tatarica</em>) compared to sheep (<em>Ovis aries</em>) to assess the impact of long-term captivity on bone characteristics. Mechanical testing was conducted to determine the compressive elastic modulus (<em>E</em>) and ultimate stress (<em>σ</em>_max) of cancellous and cortical bone. Additionally, finite element analysis (FEA) based on 3D models from Micro-CT scans was used to simulate stress distribution during running at full speed in saiga antelope.</div></div><div><h3>Results</h3><div>The mechanical testing results revealed that the cortical bone of the saiga antelope calcaneus had a significantly higher elastic modulus (<em>E</em>) than that of sheep (<em>P</em> &lt; 0.05), indicating increased stiffness. However, no significant differences were observed in the cancellous bone properties. FEA showed high stress concentrations in the midshaft region of the saiga antelope calcaneus during running simulations, where the stress exceeded the calcaneal ultimate stress (<em>σ</em>_max), suggesting a heightened risk of fracture.</div></div><div><h3>Conclusions</h3><div>The findings indicate that long-term captivity may reduce the robustness of the saiga antelope calcaneus, particularly in dynamic situations like running. This highlights the need for better management strategies, such as dietary adjustments and increased physical activity, to enhance the bone health and fitness of saiga antelope for potential reintroduction into the wild.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105858"},"PeriodicalIF":1.8,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal prevalence and in vitro sensitivity to macrocyclic lactones and imidazothiazoles in gastrointestinal nematodes in captive Addax nasomaculatus antelope","authors":"Carina Esteves ,&nbsp;Matías Villagrán ,&nbsp;Oscar Correa ,&nbsp;Gonzalo Suárez","doi":"10.1016/j.rvsc.2025.105860","DOIUrl":"10.1016/j.rvsc.2025.105860","url":null,"abstract":"<div><div>The antelope <em>Addax nasomaculatus</em> is a wild ruminant that is critically endangered and difficult to manipulate in vivo. There is limited information regarding the gastrointestinal nematode genera (GIN) present in the species and their pharmacological sensitivity to antiparasitic drugs. This study aimed to evaluate the seasonal prevalence of gastrointestinal nematode genera in the addax antelope and its relationship with in vitro sensitivity to macrocyclic lactones and imidazothiazoles in captivity. Fecal samples were collected monthly over a one-year period (<em>n</em> = 10) from a population located in Parque Lecocq (Montevideo, 34°47′S, 56°20′W) and cultured to obtain larvae 3 (L3). Larval motility was determined using a Microtracker, which was used to establish the sensitivity of L3 to seven dilutions in 1.6 % DMSO of ivermectin (range 0.5 to 32 μM), moxidectin (range 0.5 to 32 μM), and levamisole (range 1.56 to 100 μM), and the surviving L3 genera were identified by morphological traits. <em>Haemonchus</em> sp. predominated in summer, and <em>Trichostrongylus</em> sp. in winter. Overall, the effectiveness was lower than 25 % for ivermectin and moxidectin and greater than 90 % for levamisole, except during winter when <em>Trichostrongylus</em> sp. predominated. This study describes a system for indirect monitoring of in vitro pharmacological sensitivity in GIN parasite populations present in <em>Addax nasomaculatus</em>, which simultaneously seems promising for therapeutic development in other wildlife species. The results of this research contribute significantly to the understanding of deworming management in wildlife species and provide an alternative tool for research on parasite control in other wildlife species.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105860"},"PeriodicalIF":1.8,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcutaneous hyalohyphomycosis in a domestic cat caused by Flavodon flavus, an under-recognized fungal pathogen","authors":"J.A.B. Grandinetti ,&nbsp;A.N. Etchecopaz ,&nbsp;J. Maito ,&nbsp;M.L. Cuestas ,&nbsp;C. Wright","doi":"10.1016/j.rvsc.2025.105859","DOIUrl":"10.1016/j.rvsc.2025.105859","url":null,"abstract":"<div><div>This report describes the first documented case of subcutaneous hyalohyphomycosis caused by <em>Flavodon flavus</em> in a domestic cat. A 3-year-old spayed indoor cat presented with progressive cutaneous nodules on all four limbs. Histopathology revealed a pyogranulomatous inflammatory response with fungal elements, and fungal culture followed by molecular identification confirmed <em>F. flavus</em> as the etiologic agent. The cat tested negative for FIV and FeLV. Initial treatment with oral itraconazole (5 mg/kg/day) resulted in partial improvement. Due to stagnation in clinical response by day 90, oral potassium iodide (5 mg/kg/day) was added. Combined therapy led to complete resolution of lesions by day 135, and treatment was extended for an additional 60 days. No recurrence has been observed to date. This case highlights the emerging role of <em>F. flavus</em>—an environmental basidiomycete previously unrecognized as a feline pathogen—as a potential cause of subcutaneous mycoses in cats. Long-term antifungal treatment and close monitoring are recommended due to the risk of relapse.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105859"},"PeriodicalIF":1.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144851885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of mink spleen infected by Aleutian mink disease virus: Insight into immune response","authors":"D. Gregory Bishop ,&nbsp;A. Hossain Farid ,&nbsp;Duy Ngoc Do ,&nbsp;Younes Miar","doi":"10.1016/j.rvsc.2025.105842","DOIUrl":"10.1016/j.rvsc.2025.105842","url":null,"abstract":"<div><div>Aleutian disease (AD) is one of the most challenging mink diseases that causes high mortality and affects several economically important traits. Aleutian mink disease virus (AMDV) targets multiple organs, the spleen being one of the major targeted organs. Transcriptomics has been widely used to reveal genes and biological pathways and to identify biomarkers for early detection or prevention of diseases. This study aimed to identify genes and pathways related to the host response to AD infection in the spleen using transcriptomics. For this purpose, twelve AMDV-free black male mink (3 sets of 4 full-sibs) were housed for 4–7 days pre-inoculation and then were intranasally inoculated with viral homogenate and euthanized at 24 h (Day 1), 48 h (Day 2), and Day 7 post-infection. Spleen tissues were collected from infected and control (Day 0) mink for RNA isolation. Libraries were prepared (Illumina TruSeq™ RNA kit) and sequenced (HiScanSQ, 101 bp paired-end reads). After cleaning with Cutadapt v1.4.2, 942,803,540 high-quality reads remained for analysis. Differential gene expression analyses revealed that the highest number of significant differentially expressed (DE) genes were observed between Day 1 and Day 0 (168) while the fewest DE genes (23) were observed between Day 7 and Day 0. A total of 19 DE genes are identified between three observation pair comparisons and eight of them are directly involved in immune response (<em>FGL2, TLF8, LRP1, SERPINB9, MSR1, C3, PLA2R1</em>, and <em>XCR1)</em>. Gene enrichment analyses revealed pathways related to innate immune and lipid metabolism which are important mechanisms for the host to fight against infections. One of the important pathways is neutrophil degranulation which functions as aiding in the elimination of pathogens and the initiation of the inflammatory process. In conclusion, the current study provides insight into the transcriptomic profiles of the spleen in mink infected with AMDV. Identified candidate genes might be used for functional studies or as prior information for markers or genomic selection against AD in mink. Further studies in other tissues or single-cell RNA sequencing might deliver more comprehensive pictures of host responses to AMDV infections.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105842"},"PeriodicalIF":1.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol ameliorates Salmonella Typhimurium-induced intestinal inflammation and barrier dysfunction in chickens via COX-2 inhibition","authors":"Yaochen Xie,&nbsp;Shenghong Chen,&nbsp;Dingqian Guo,&nbsp;Hui Peng,&nbsp;Xuhua Ran,&nbsp;Xiaobo Wen","doi":"10.1016/j.rvsc.2025.105843","DOIUrl":"10.1016/j.rvsc.2025.105843","url":null,"abstract":"<div><div><em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Typhimurium (<em>Salmonella</em> Typhimurium) is a prominent foodborne pathogen that elicits considerable morbidity and mortality in poultry. This study explored the potential of resveratrol (RES) as a treatment for <em>S.</em> Typhimurium infection, focusing on its effects in Wenchang chickens. We tested three doses of RES (50, 100, and 200 mg/kg body weight) to assess its impact on inflammatory cytokine levels, intestinal barrier function, growth performance, and clinical signs in infected chickens. In the group treated with 200 mg/kg of RES, we observed a marked improvement in feed conversion ratio (FCR) and average daily gain (ADG) between days 7 and 14 post-infection. Both the 100 mg/kg and 200 mg/kg doses helped restore the intestinal barrier, reversing the damage caused by <em>S.</em> Typhimurium and normalizing the villus height-to-crypt depth (VH:CD) ratio in the jejunum. Additionally, the 200 mg/kg group showed enhanced repair of the intestinal barrier, with a significant reduction in the expression of pro-inflammatory cytokines (IL-6, TNF-α, TGF-β) and an increase in the levels of tight junction proteins (ZO-1, Occludin, Claudin-1). RES decreased COX-2 mRNA expression in intestinal epithelial cells, thereby reducing the activation of prostaglandin-related inflammatory pathways. In vitro experiments using <em>S.</em> Typhimurium-infected HD11 macrophages showed that RES inhibited both COX-2 enzymatic activity and the release of inflammatory cytokines (TNF-α, IL-1β, IL-6, and TGF-β) in a dose-dependent manner. In conclusion, these results highlight RES as a promising natural treatment for <em>S.</em> Typhimurium infection in poultry.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105843"},"PeriodicalIF":1.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-to-lymphocyte, monocyte-to-lymphocyte, and platelet-to-lymphocyte ratios as prognostic markers in canine osteosarcoma: Correlation with cytological and histopathological features","authors":"Gabriela de Almeida Fernandes e Silva ,&nbsp;Juliana Jurado Jimenez ,&nbsp;Fernanda Barthelson Carvalho de Moura ,&nbsp;Thays de Souza Gomes ,&nbsp;Francisco Javier Pedraza Ordoñez ,&nbsp;Luis Mauricio Montoya Florez ,&nbsp;Luis Fernando Barbisan ,&nbsp;Rogerio Antonio de Oliveira ,&nbsp;Carlos Mario Gonzalez Zambrano ,&nbsp;Luciane Alarcão Dias-Melicio ,&nbsp;Noeme Sousa Rocha","doi":"10.1016/j.rvsc.2025.105846","DOIUrl":"10.1016/j.rvsc.2025.105846","url":null,"abstract":"<div><div>Canine osteosarcoma is a highly aggressive neoplasm with a complex tumor microenvironment influenced by inflammatory and cytomorphological factors. This study aimed to evaluate the prognostic value of systemic and tumor-associated inflammatory indices, along with cytological and histopathological characteristics, in predicting clinical outcomes. A retrospective cohort of canine osteosarcoma cases was analyzed, assessing the neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), and platelet-to-lymphocyte ratio (PLR), in addition to cytopathological and histopathological features, as well as NLR values quantified in cytology. The results indicated that early clinical intervention significantly improved postoperative survival, reinforcing the importance of timely diagnosis. Both NLR and LMR showed significant correlations with tumor progression and prognosis, supporting their utility as continuous monitoring tools Tumor-associated NLR was linked to neutrophilic infiltration and osteoid content, suggesting involvement of the inflammatory response in tumor progression. Moreover, tumor-associated NLR inversely correlated with overall and postoperative survival times, supporting its association with tumor aggressiveness beyond cytological and histological features. Cytological and histopathological analyses highlighted nuclear pleomorphism and cellular density as potential malignancy indicators, supporting cytology as an accessible and cost-effective diagnostic tool. High vascular involvement was observed, reinforcing the hypothesis of a pro-angiogenic microenvironment contributing to tumor invasiveness. These findings emphasize the translational relevance of inflammatory indices and cytomorphological parameters in veterinary oncology, providing insights into tumor biology and potential therapeutic stratification strategies. Further studies are required to refine these biomarkers and integrate them into clinical protocols and comparative oncology research.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105846"},"PeriodicalIF":1.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in canine iPSC technology: Current methods and future directions — A narrative review","authors":"Pruettha Aruvornlop ,&nbsp;Warunya Chakritbudsabong ,&nbsp;Nichawadee Sandech ,&nbsp;Tharathip Muangthong ,&nbsp;Sasitorn Rungarunlert","doi":"10.1016/j.rvsc.2025.105844","DOIUrl":"10.1016/j.rvsc.2025.105844","url":null,"abstract":"<div><div>Canine induced pluripotent stem cells (ciPSCs) hold significant promise for veterinary regenerative medicine and as translational models for human diseases. Deriving these cells from somatic sources also circumvents ethical concerns associated with embryonic material. However, progress in the field is impeded by challenges such as low reprogramming efficiency, lack of standardization, unique epigenetic barriers, and ambiguous pluripotency states. This narrative review provides a comprehensive and up-to-date overview of current approaches to ciPSC generation and characterization, emphasizing recent advancements in donor cell selection, reprogramming methodologies, culture conditions, and analytical techniques. Furthermore, the review explores emerging applications in regenerative medicine and disease modeling, discussing both future potential and current limitations. Addressing these challenges through improved protocols and effective differentiation strategies is crucial to fully unlocking ciPSC technology's research and therapeutic value.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105844"},"PeriodicalIF":1.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144851884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Recombinant yeast as an oral vaccine carrier - direct in vivo interaction with chicken gut epithelium","authors":"José M. Jaramillo-Ortiz,&nbsp;Samruddhi A. Deosthali,&nbsp;Damer P. Blake,&nbsp;Dirk Werling","doi":"10.1016/j.rvsc.2025.105845","DOIUrl":"10.1016/j.rvsc.2025.105845","url":null,"abstract":"<div><div>Oral vaccines present a strategy for mass-immunisation, offering ease of administration with the potential to induce mucosal and systemic immunity. The yeast <em>Saccharomyces cerevisiae</em> is a highly scalable platform for protein expression and delivery, with strong potential for use as an oral vaccine due to its established safety profile and immunomodulatory properties. In this study, we explored the interaction between <em>S. cerevisiae</em> expressing surface-displayed mCherry proteins and the chicken gastrointestinal tract (GIT). Orally inoculated Hy-Line Brown chickens were sacrificed 1.5 hs post-gavage, and precision cut intestinal slices (PCIS) were analysed using fluorescence microscopy. Fluorescent yeast particles were observed adhered to epithelial cells in PCIS generated from duodenum and jejunum, suggesting specific host-yeast interactions. No such interaction was observed in control animals. To our knowledge, our data are the first visual demonstration of yeast interacting with the avian intestinal epithelium. Our findings highlight the potential of live and inactivated <em>S. cerevisiae</em> as a safe and effective platform for oral vaccine delivery in poultry, warranting further investigation into its interaction with gut-associated immune tissues.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105845"},"PeriodicalIF":1.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}