Research in veterinary science最新文献

{"title":"Epidemiology and genetic characterization of myxoma virus from the first outbreak in Finland","authors":"Ari Kauppinen ,&nbsp;Marja Isomursu ,&nbsp;Henna Pekkarinen ,&nbsp;Minna Nylund ,&nbsp;Ilona Laamanen ,&nbsp;Tiina Nokireki ,&nbsp;Tuija Gadd","doi":"10.1016/j.rvsc.2025.105544","DOIUrl":"10.1016/j.rvsc.2025.105544","url":null,"abstract":"<div><div>Myxomatosis, caused by myxoma virus (MYXV), is a fatal disease of the European rabbit (<em>Oryctolagus cuniculus</em>). It has a worldwide distribution and has become an endemic disease throughout Europe since its introduction into the rabbit population in 1950. This study describes the first outbreak caused by MYXV in Finland in summer 2020. Eighteen feral rabbits collected during the outbreak and two during the following year were confirmed positive for MYXV using real-time PCR. Coinfection with rabbit hemorrhagic disease virus (RHDV) was observed in six cases. Whole genome sequencing of MYXV was performed for five rabbit samples collected during the outbreak in 2020 and two rabbit samples collected in 2021 to assess the origin of the virus and possible evolutionary mutations. Genetic characterization revealed that the genomes of the five samples collected during the outbreak in 2020 were identical. Phylogenetic analysis demonstrated the highest nucleotide identity to the German field strain FLI-H (99.97 %). The Finnish strains contain mutations that have not previously been described and may be responsible for changes in virus properties. On the other hand, the virus genome displayed only minor changes during the one-year follow-up in Finland. These findings add to knowledge of the spread of MYXV and its evolution.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105544"},"PeriodicalIF":2.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epitheliotropic T-cell lymphoma of non-viral origin with hypercalcemia of malignancy in a Roman goose (Anser anser domesticus)","authors":"Giuseppe Giglia ,&nbsp;Ilaria Porcellato ,&nbsp;Manuela Spinosa ,&nbsp;Monica Sforna","doi":"10.1016/j.rvsc.2025.105549","DOIUrl":"10.1016/j.rvsc.2025.105549","url":null,"abstract":"<div><div>A Roman goose (<em>Anser anser domesticus</em>) was presented with a large mass on the left lateral side of the neck with a smaller satellite mass on the right. Utilizing radiography, blood tests, and histological and immunohistochemical investigations, a diagnosis of cutaneous T-cell lymphoma associated with hypercalcemia of malignancy was established. The involvement of oncogenic viruses was ruled out through PCR and RT-PCRs. Due to the mass's considerable size, location, and post-treatment management challenges, the owner opted against surgery and chemotherapy. Glucocorticoids were administered upon the great enlargement of a second mass on the base of the right side of the neck and the worsening of clinical signs but proved entirely ineffective. Due to the disease progression, euthanasia was elected, and a post-mortem investigation ensued to clarify the extent and confirm the lesion's nature. Similar histological and immunohistochemical findings confirmed the final diagnosis of epitheliotropic T-cell lymphoma with cutaneous and esophageal involvement.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105549"},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the bovine mastitis co-infections: Coexistence with Enterobacter alters S. aureus antibiotic susceptibility and virulence phenotype","authors":"Paula Isaac ,&nbsp;María Laura Breser ,&nbsp;María Florencia De Lillo ,&nbsp;Luciana Paola Bohl ,&nbsp;Luis Fernando Calvinho ,&nbsp;Carina Porporatto","doi":"10.1016/j.rvsc.2025.105547","DOIUrl":"10.1016/j.rvsc.2025.105547","url":null,"abstract":"<div><div>The World Health Organization recently reported an alarming evolution and spread of antibiotic resistance, a global risk factor recognized as a One Health challenge. In veterinary, the general lack of clear treatment guidelines often leads to antibiotic misuse. Bovine mastitis is responsible for major economic losses and the main cause of antibiotic administration in the dairy industry, favoring the emergence of multi-resistant phenotypes. The complexity of inter-microbial and host-pathogen interactions in the mammary gland, demonstrated by culture-independent techniques, not only complicates the prediction of antibiotic treatment outcomes but also underscores the urgent need for further research in this field. This work evaluated the interactions between <em>S. aureus</em> L33 and <em>Enterobacter</em> sp. L34 obtained from an intramammary co-infection. The behavior of the dual-species culture resembled that of the <em>Enterobacter</em> monoculture in all the evaluated contexts. Most of the selected <em>S. aureus</em> virulence factors and the antibiotic susceptibility were altered by coexisting with <em>Enterobacter</em>. Under the protection of <em>Enterobacter</em>, <em>S. aureus</em> was able to survive upon exposure to concentrations of cloxacillin and other antibiotics that would be bactericidal for the monoculture. This could have serious implications for bacterial clearance of mastitis originating from the underestimated co-infections. These findings highlight the importance of broadening our knowledge of how microbial interactions in intramammary infections could contribute to antibiotic treatments failures. Moreover, they open new perspectives for the design of bovine mastitis therapies that consider the ecological context in order to optimize the antibiotic usage, improve the success rates and reduce antibiotic resistance.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105547"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cartilage defect identification on computed tomography arthrography in equine fetlock: Ex-vivo study","authors":"Irene Nocera ,&nbsp;Chiara Di Franco ,&nbsp;Elisa Marcucci ,&nbsp;Caterina Puccinelli ,&nbsp;Giulia Sala ,&nbsp;Micaela Sgorbini ,&nbsp;Simonetta Citi","doi":"10.1016/j.rvsc.2025.105548","DOIUrl":"10.1016/j.rvsc.2025.105548","url":null,"abstract":"<div><div>CT arthrography (CTA) for evaluating cartilage defects would be beneficial in clinical practice. Since the contrast medium (CM) volume is often driven by operator experience, the aim was to evaluate the minimum volume of iodinated CM for CTA sufficient to identify iatrogenic cartilage defects in horse fetlock. The study was conducted on <em>ex-vivo</em> 32 distal limbs from adult horses collected at slaughter. Iatrogenic cartilage defects were arthroscopically made on fetlock, at 5 predetermined locations. Subsequently, CT scans were performed, according to the following protocol: precontrast scan, followed by subsequent scans with incremental volumes of CM, ranging from 2,5–40 ml (total of 10 scans per fetlock). Then, the cartilage defects were measured macroscopically using a calliper. CTA scans were evaluated by a single operator, and defects were assessed for presence/absence and volume measures. Data were expressed as median and interquartile values. The association between CT scan assessments and different CM volumes was examined with chi-square test. The correlation between CT scans and macroscopic assessments was evaluated using Spearman's coefficient. Significance was set at a <em>p</em>-value &lt;0.05. Twenty millilitres of CM were required to visualize 100 % of the defects, regardless of the location. A weak correlation was found between the defects' volume on macroscopic and CT scan assessment. Twenty millilitres of CM was sufficient to identify cartilage defects on the fetlock articular surface, supporting the conscious use of CM adequate for specific diagnostic purposes.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105548"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Biosynthesis and regulation mechanisms of the Pasteurella multocida capsule” [Res Vet Sci. 127 (2019):82–90/ Doi: 10.1016/j.rvsc.2019.10.011]","authors":"Lijun Guan ,&nbsp;Yun Xue ,&nbsp;Wenwen Ding ,&nbsp;Zhanqin Zhao","doi":"10.1016/j.rvsc.2025.105542","DOIUrl":"10.1016/j.rvsc.2025.105542","url":null,"abstract":"","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105542"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinarians approach towards antimicrobial stewardship and one health: A survey study","authors":"Falguni S. Thakar ,&nbsp;Kiran Vasava ,&nbsp;Snehal Bagtharia ,&nbsp;Amit Kanani ,&nbsp;Vartika Chandra ,&nbsp;Beena Patel ,&nbsp;Dipeeka K. Mandaliya","doi":"10.1016/j.rvsc.2025.105546","DOIUrl":"10.1016/j.rvsc.2025.105546","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is one of the top 10 global public health threats. One Health approach is needed to tackle antimicrobial resistance as most antimicrobials used in animals are also used in humans and affect the environment. Although the knowledge and attitudes of personnel in the human healthcare sector are studied more frequently in Gujarat, veterinarians' approaches also play a pivotal role in attaining antimicrobial stewardship. Thus, an online survey was conducted to understand the knowledge, attitudes, and practices of veterinarians regarding AMR and antimicrobial stewardship in Gujarat. A total of 691 respondents included 300 government veterinarians (44 %), 258 cooperative veterinarians (37 %), 45 private veterinarians (5 %), 69 MVD GVK veterinarians (10 %), and 19 others (4 %). Several veterinarians (77 %) believe that farmers' misuse of antimicrobials contributes significantly to AMR. According to 78 % of respondents, the inappropriate use of antimicrobials in food-producing animals contributes to AMR, which can be reduced by vaccines, biosecurity measures, and hygiene (79 %). As per 86 % of the responders, antimicrobial stewardship is important, and 81 % educate farmers on the rational use of antimicrobials. Though 92 % of veterinarians agreed that culture and antimicrobial sensitivity testing (AST) should be done as frequently as possible to guide antimicrobial usage, only 4 % always, and 68 % sometimes send samples for AST. However, 31 % think it is acceptable to commence antimicrobial therapy without a confirmatory diagnosis. The study findings suggest that AMR awareness among farmers, increased provision of laboratory services, and AST at affordable costs should be developed to combat the development of AMR.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105546"},"PeriodicalIF":2.2,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A longitudinal study on the detection of anti-Leishmania antibodies in a captive European mink (Mustela lutreola) population and their correlation with serum protein electrophoresis","authors":"María del Carmen Aranda ,&nbsp;José Villora ,&nbsp;Jacobo Giner ,&nbsp;Antonio Fernández ,&nbsp;Pablo Quilez ,&nbsp;Janine Elizabeth Davies ,&nbsp;María D. Pérez ,&nbsp;Sergio Villanueva-Saz ,&nbsp;Diana Marteles","doi":"10.1016/j.rvsc.2025.105541","DOIUrl":"10.1016/j.rvsc.2025.105541","url":null,"abstract":"<div><div>Leishmaniosis, caused by <em>Leishmania infantum</em> and transmitted by sand flies, is a significant zoonotic disease. Dogs are primary reservoirs, but other domestic animals, such as cats and ferrets, and wild species, including Eurasian otters and European mink, can be hosts. This study focused on European mink, a critically endangered species, investigating the seroprevalence of <em>L.</em> <em>infantum</em> antibodies and its correlation with serum protein profiles over 2021–2024. A total of 68 captive minks in Spain were monitored, yielding 105 serum samples. ELISA tests identified 44 seropositive samples (41.9 %), with seroprevalence varying annually. While no significant association was found between seropositivity and age, gender, or body condition, seropositivity correlated significantly with the year of collection. Some animals exhibited fluctuating antibody levels across sampling periods. Serum protein electrophoresis analyzed 96 samples, revealing that <em>L.</em> <em>infantum</em> seropositivity significantly affected total protein concentrations, β-globulins, γ-globulins, and albumin:globulin ratio. Variations were also observed in specific protein fractions among both seropositive and seronegative minks. These findings highlight European mink's potential epidemiological role in <em>L.</em> <em>infantum</em> transmission. Monitoring antibody levels and protein profiles in captive populations can inform conservation strategies and disease management efforts.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105541"},"PeriodicalIF":2.2,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enniatin B alters bovine polymorphonuclear leukocytes phagocytosis and extracellular reactive oxygen species production in vitro","authors":"S. Sandrini,&nbsp;G. Salvi,&nbsp;D. Ravanelli ,&nbsp;V. Perricone,&nbsp;L.G. De Matos,&nbsp;F. Ceciliani,&nbsp;C. Lecchi,&nbsp;A. Agazzi","doi":"10.1016/j.rvsc.2025.105540","DOIUrl":"10.1016/j.rvsc.2025.105540","url":null,"abstract":"<div><div>Enniatins (<strong>ENNs</strong>) affect human and animal health. Different ENN analogs have been identified, but Enniatin B (<strong>ENN B</strong>) is the most detected in foods and feeds. This study investigated the effect of ENN B on bovine polymorphonuclear leukocytes (<strong>PMNs</strong>) challenged with increasing ENN B concentrations (0.625, 1.25, 2.5, 5, and 10 μM). Bovine PMNs were isolated from the peripheral blood of dairy cows to evaluate the cell viability, chemotactic function, ability to phagocyte Gram+ and Gram– microorganisms, and extracellular Reactive Oxygens Species (<strong>ROS</strong>), with or without phorbol 12-myristate-13-acetate (<strong>PMA</strong>) as a pro-inflammatory challenge. Results demonstrated that ENN B did not affect bovine PMN viability and chemotactic activity at all concentrations (<em>p</em> = 0.952; <em>p</em> = 0.218, respectively). <em>E. coli</em> and <em>S. aureus</em> phagocytosis ability were reduced by ENN B at the highest concentrations (5 and 10 μM) compared to the negative control (<em>p</em> ≤ 0.001; <em>p</em> = 0.001, respectively). Extracellular ROS production was increased by ENN B challenge under physiological and pro-inflammatory conditions (<em>p</em> = 0.014; <em>p</em> &lt; 0.001, respectively). In conclusion, ENN B did not exert cytotoxic effects on bovine PMNs, while reduced phagocytic ability and increased the production of extracellular ROS, highlighting its potential role as an immunomodulator of the bovine innate immune response <em>in vitro</em>.</div></div><div><h3>Implications</h3><div>Emerging mycotoxin Enniatin B is a common grain contaminant worldwide that can exert cytotoxic and immunotoxic effects in animal cells. We hypothesized that Enniatin B could <em>in vitro</em> affect the bovine immune response. In our study, Enniatin B did not affect bovine polymorphonuclear cell viability and chemotaxis, while a reduction of phagocytosis and a modulation of extracellular reactive oxygen species were observed. The present study shows that Enniatin B <em>in vitro</em> exerts a potential role as an immunomodulator of the bovine innate immune response putting animals at an increased risk of infection diseases.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105540"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a duplex loop-mediated isothermal amplification together with lateral flow dipstick assay for the detection and discrimination of parasitic infections in chickens between cestodes belonging to genus Raillietina and trematodes in family Echinostomatidae","authors":"Wasin Panich ,&nbsp;Thanawan Tejangkura ,&nbsp;Thapana Chontananarth","doi":"10.1016/j.rvsc.2025.105539","DOIUrl":"10.1016/j.rvsc.2025.105539","url":null,"abstract":"<div><div>Most poultry farming that has been conducted by smallholders in Thailand are free-range and housing systems, which have risks of parasitic infection from the environment, particularly from tapeworms in the genus <em>Raillietina</em> and trematodes in the family Echinostomatidae. These have become important health problems in the poultry industry, causing low feed conversion ratios and leading to the loss of economic value. Our objective was to develop and validate a molecular technique based on duplex loop-mediated isothermal amplification (LAMP) together with lateral flow dipstick (LFD) assay for discriminating the infections between the genus <em>Raillietina</em> (<em>R. echinobothrida</em>, <em>R. tetragona</em>, and <em>R. cesticillus</em>) and the family Echinostomatidae (<em>E. miyagawai</em>, <em>E. mekongi</em>, <em>E. macrorchis</em>, and <em>H. conoideum</em>) in a single reaction. The developed assay was highly specific without cross-amplification with other poultry helminths and their hosts, at the optimized condition of 66 °C for 80 min. In addition, the results could be clearly visualized with the naked eye via LFD after incubating with probes at 66 °C for 10 min. The detection limit or analytical sensitivity of the <em>Raillietina</em> and Echinostomatidae groups were found to be 5 × 10⁻⁴ and 5 × 10⁻² ng/μL, respectively. In clinical tests, the developed assay successfully detected parasites in naturally infected faeces from chickens in Thailand; results from McNemar's tests revealed no significant difference when compared to standard microscopy methods. Therefore, our assay is a viable alternative technique for an accurate and convenient diagnosis; it can also be used as a tool to guide anthelmintic drugs decision-making for treatments and farm management.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"185 ","pages":"Article 105539"},"PeriodicalIF":2.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical targets of chick embryos affected by sub-lethal concentrations of lambda-cyhalothrin and imidacloprid","authors":"Nagat M. Aly,&nbsp;Anter K. Mahmoud,&nbsp;Eman M. Mosallam","doi":"10.1016/j.rvsc.2025.105538","DOIUrl":"10.1016/j.rvsc.2025.105538","url":null,"abstract":"<div><div>The insecticides Lambda-cyhalothrin (LCT) and imidacloprid (IMD) are extensively utilized in Egyptian agriculture. Embryonic chicken is a readily accessible model organism commonly employed in various studies. Eggs of (<em>Gallus Gallus</em>) chicken were immersed in an aqueous solution of two sub-lethal concentrations (0.375 and 0.0375 mg/L for LCT; 0.05 and 0.005 mg/L for IMD) for 30  sec on the fourth day of incubation of chick embryos. Significant reductions of acetylcholinesterase (AChE) activity of brain 18- and 21-day chicks were observed in the groups treated with LCT and IMD dependent on concentrations. There were significant changes (reduction or enhancement) in serum activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) by LCT and IMD at the used concentrations, while non-significant stimulation in the AST/ALT ratio (AAR) was found. In 18th - day embryos, the activities of glutathione S-transferase (GST) and acid phosphatase (ACP) were not significantly changed by LCT but were significantly increased by IMD. Liver alkaline phosphatase (ALP) activity showed no significant change except IMD at 0.05 mg/L. However, serum enzyme activity was significantly reduced in all groups. In addition, the tested insecticides caused notable increases in the creatinine and total protein content. The protein profile; proteins separation with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed an increase in four bands that are consistent with the results of the biomarkers level. Findings indicate that even the pesticide's low concentrations are not safe and may lead to severe damage to the embryos and may lead to significant harm or developmental disruption in the embryos.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105538"},"PeriodicalIF":2.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}