Research in veterinary science最新文献

{"title":"Strategies for combating FIPV infection: antiviral agents and vaccines","authors":"Fan Gao,&nbsp;Guilan Wen","doi":"10.1016/j.rvsc.2025.105709","DOIUrl":"10.1016/j.rvsc.2025.105709","url":null,"abstract":"<div><div>Feline infectious peritonitis virus (FIPV) is a deadly virus that causes feline infectious peritonitis (FIP) in cats. FIP is a biotype of feline coronavirus (FCoV). Currently, the prevention and treatment of FIPV is challenged by the absence of adequate clinical treatment drugs and vaccines that offer strong immune protection complicated. In this review, we analyse and explain how small-molecule inhibitors function to protect against viral infections. Additionally, we highlight the challenges and future possibilities in developing FIPV vaccines, which are crucial for enhancing FIPV treatment and creating viable vaccine solution strategies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105709"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does hypertriglyceridemia induce increased oxidative stress in obese dogs?","authors":"Yuri Lima Pereira ,&nbsp;Laura Elisa Raviolo ,&nbsp;Gabriela Fernanda Vitoretti Machado ,&nbsp;Danielle Venturini ,&nbsp;Karina Keller Marques da Costa Flaiban","doi":"10.1016/j.rvsc.2025.105723","DOIUrl":"10.1016/j.rvsc.2025.105723","url":null,"abstract":"<div><div>Obesity is considered the most common nutritional disorder in pets. It triggers a low-grade inflammatory process that can lead to increased oxidative stress and contribute to various diseases. The purpose of this study was to evaluate the systemic levels of oxidative stress biomarkers in dogs with overweight, obesity and ideal body condition score (BCS). Fifty-eight adult and senior dogs were selected and divided into three groups: overweight (n = 20), obese (n = 20), and ideal BCS (n = 18). Oxidative stress biomarkers were measured using lipid peroxidation products, by ferrous oxidation-xylenol orange (FOX), advanced oxidative protein products (AOPP), and total reactive antioxidant potential (TRAP), with C-reactive protein (CRP) concentration used as an inflammatory biomarker. Protein oxidation was observed in overweight and obese dogs, as evidenced by elevated AOPP levels (p = 0.004) and their ratio to antioxidant levels (AOPP/TRAP) through the prooxidant-antioxidant index (PAI) (p = 0.015) in obese dogs. In obese dogs with hypertriglyceridemia, lipid oxidation (p = 0.018) was observed in addition to protein oxidation (p &lt; 0.001). Furthermore, a positive correlation was observed between the triglyceride levels and AOPP (r = 0.716, p &lt; 0.001) and PAI (r = 0.749, p &lt; 0.001) concentrations. Finally, we concluded that obesity alone may not be sufficient to induce increased oxidative stress and that hypertriglyceridemia is correlated with protein and lipid oxidation in obese dogs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105723"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of chickens with probiotics under conditions conducive to necrotic enteritis development","authors":"Mohammadali Alizadeh ,&nbsp;Charlotte Fletcher ,&nbsp;Samson Oladokun ,&nbsp;Amirul I. Mallick ,&nbsp;Khaled Abdelaziz ,&nbsp;Myles St-Denis ,&nbsp;Sugandha Raj ,&nbsp;Katherine Blake ,&nbsp;Shayan Sharif","doi":"10.1016/j.rvsc.2025.105711","DOIUrl":"10.1016/j.rvsc.2025.105711","url":null,"abstract":"<div><div>Necrotic enteritis (NE) poses a significant challenge to the global broiler industry, particularly with the increasing restrictions on using antibiotic growth promoters. Probiotics have emerged as a promising alternative for effective disease control. This study evaluated the efficacy of a probiotic cocktail consisting of <em>Lactobacillus crispatus</em>, <em>Ligilactobacillus johnsonii</em>, <em>Limosilactobacillus reuteri</em>, and 2 strains of <em>Ligilactobacillus salivarius</em>, under experimental conditions conducive to NE. Chickens were divided into two groups based on stocking density: high stocking density (30 birds/m²) and normal stocking density (15 birds/m²). Within each group, one subgroup received 10⁸ colony-forming units (CFUs) of lactobacilli on days 1, 7, 14, and 20 of age, while the other received phosphate-buffered saline. Body weight and lesion scores were recorded on days 21 and 24, respectively. Tissues from the intestine were collected for analysis of immunoregulatory genes and lymphocyte population. Cecal contents were collected for microbiome analysis. Probiotic treatment improved body weight gain compared to non-treated controls and reduced gut lesion scoring in the birds raised under high stocking density. Probiotic treatment increased the frequency of Bu-1⁺ B cells and CD3⁺CD4⁺ T cells in the cecal tonsils and enhanced the relative expression of antimicrobial peptides (zonula occludens and occludin) in the ileum. However, it decreased the expression of heat shock proteins, interleukin (IL)-18, IL-1β, and interferon (IFN)-γ. Probiotics also enhanced alpha diversity and the abundance of <em>Christensenellaceae</em>_R-7 group, <em>Angelakisella</em>, and <em>Clostridia_vadinBB60</em> group compared to the high stocking density control group. These findings underscore the potential of probiotics to mitigate NE in broiler chickens, particularly under conditions of high stocking density.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105711"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The presence of acylated homoserine lactones and diffusible signal factor in bronchoalveolar lavage fluid from horses with clinical exacerbation of severe equine asthma","authors":"Neza Mrzdovnik ,&nbsp;Janja Babič ,&nbsp;Dane Lužnik ,&nbsp;Dušan Žigon ,&nbsp;Matic Mrzdovnik ,&nbsp;Gabrijela Tavčar-Kalcher ,&nbsp;Viktorija Tomič ,&nbsp;Francis Prescott John ,&nbsp;Modest Vengust","doi":"10.1016/j.rvsc.2025.105720","DOIUrl":"10.1016/j.rvsc.2025.105720","url":null,"abstract":"<div><div>Several bacteria associated with chronic lung pathology use quorum sensing (QS) signaling molecules to regulate their virulence in pure cultures and poly-microbial communities. Their excessive growth and biofilm formation in the respiratory tract increase the morbidity and mortality of inflammatory airway diseases in humans, such as chronic obstructive pulmonary disease (COPD), asthma and cystic fibrosis (CF). In horses, severe equine asthma (SEA) has many parallels to these human diseases. We hypothesized that QS molecules associated with the most common biofilm-forming lung pathogens in humans (<em>Pseudomonas aeruginosa, Stenotrophomonas maltophilia</em>) may also be present in the lungs of horses with SEA.</div><div>Samples of bronchoalveolar lavage fluid (BALf) were taken from twenty horses with exacerbated SEA. Microbiological cultures of the BALf samples were performed. Liquid chromatography coupled with tandem mass spectrometry was used to identify C4-HSL, C6-HSL, 3-oxo-C12-HSL and 11-methyl-2-dodecenoic acid, which are associated with the QS mechanisms of <em>Pseudomonas aeruginosa</em> and <em>Stenotrophomonas maltophilia</em>.</div><div><em>Stenotrophomonas maltophilia</em> was identified in three horses. <em>Pseudomonas aeruginosa</em> was not identified in any sample. The quorum sensing molecules C4-HSL, C6-HSL, 3-oxo-C12-HSL associated with biofilm formation by <em>P. aeruginosa</em> and 11-methyl-2-dodecenoic acid associated with biofilm formation by <em>S. maltophila</em> were not detected.</div><div>It is unlikely that biofilm-forming bacterial strains associated with chronic lung disease in humans express similar virulence in SEA.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105720"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspergillus nidulans as an agent of subcutaneous mycetoma in a horse","authors":"Talita Bordoni,&nbsp;Filippo Maria Dini,&nbsp;Maria Morini,&nbsp;Riccardo Rinnovati,&nbsp;Alessandro Spadari,&nbsp;Roberta Galuppi","doi":"10.1016/j.rvsc.2025.105719","DOIUrl":"10.1016/j.rvsc.2025.105719","url":null,"abstract":"<div><div>Mycetoma is a fungal chronic skin proliferative lesion rarely encountered in horses and often associated with fungi such as <em>Scedosporium</em> spp., <em>Madurella</em> spp., <em>Phialophora</em> spp., <em>Curvularia</em> spp., and less frequently with <em>Aspergillus</em> spp. A case of subcutaneous mycetoma in the cervical region in a 7-year-old male Quarter Horse, diagnosed through cytological, histological, cultural and molecular methods, is reported. <em>Aspergillus nidulans</em>, usually implicated in guttural pouch mycosis, was identified. This unusual case represents the second case report of mycetoma caused by <em>Aspergillus nidulans</em> in the horse and highlights the importance of including mycotic diseases in the differential diagnosis of nodular skin masses in equine patients.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105719"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-port video-assisted laparoscopic ovariohysterectomy using operative endoscope or SILS™ device in dogs","authors":"Caio de Faria Tiosso ,&nbsp;Fabiana Azevedo Voorwald ,&nbsp;Maurício Veloso Brun ,&nbsp;Bárbara Gonçalves Barbosa ,&nbsp;Beatriz Ibrahim Miranda Antunes ,&nbsp;Roberta Martins Crivelaro ,&nbsp;Roberto Thiesen ,&nbsp;Marco Augusto Machado Silva ,&nbsp;Wilter Ricardo Russiano Vicente","doi":"10.1016/j.rvsc.2025.105704","DOIUrl":"10.1016/j.rvsc.2025.105704","url":null,"abstract":"<div><div>Over the last few years, minimally invasive surgery has gained increasing recognition in various veterinary fields because of its advantages over conventional surgery. This progress has stimulated the development of new instruments with potential applications in veterinary medicine. This randomized controlled study was performed to evaluate two different single-port techniques for performing video-assisted ovariohysterectomy in female dogs, comparing them with the conventional approach. Thirty female dogs (<em>n</em> = 30) were evaluated and divided into 3 groups of 10 animals each: ovariohysterectomy by celiotomy, video-assisted laparoscopic ovariohysterectomy using a single-port operative endoscope, and laparoscopic ovariohysterectomy using the single-incision laparoscopic surgery device. The mean surgical time, complications, postoperative pain levels, and serum levels of C-reactive protein and interleukin-6 were assessed. Both video-assisted techniques demonstrated high feasibility and satisfactory clinical outcomes, although minor complications were observed (4 in the operative endoscope group and 3 in the single-incision laparoscopic surgery device group). The interleukin-6 and C-reactive protein levels peaked earlier, at 12 and 24 h postoperatively, respectively. The higher pain scores observed in animals undergoing ovariohysterectomy by celiotomy indicate that pain in ovariohysterectomy procedures is directly related to visceral manipulation and traction of the ovarian pedicle.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105704"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence genes of pathogenic and clinical treatment in floppy kid syndrome","authors":"Cheng Cheng ,&nbsp;Yan Zheng ,&nbsp;Darong Cheng ,&nbsp;Jianping Tao","doi":"10.1016/j.rvsc.2025.105699","DOIUrl":"10.1016/j.rvsc.2025.105699","url":null,"abstract":"<div><div>Floppy kid syndrome (FKS) is a metabolic disorder disease in newborn goats caused by intestinal dysbiosis. In this study, pathogens were isolated from the intestinal contents of FKS-affected goats (8 <em>Escherichia coli</em> isolates and 2 <em>Staphylococcus aureus</em> isoates), and virulence gene detection showed that <em>Escherichia coli</em> predominantly expressed <em>HlyD</em> (62.5 %) and <em>K88</em> (37.5 %), with 37.5 % of isolates co-expressing <em>HlyD</em>/<em>K88</em>; all <em>Staphylococcus aureus</em> isolates carried the <em>nuc, sea, sed,</em> and <em>FnbA</em>. Antibiotic susceptibility testing revealed that amikacin exhibited the highest sensitivity against both pathogens, with sensitivity rates of 87.5 % in <em>Escherichia coli</em> and 100 % in <em>Staphylococcus aureus</em>. By combining virulence gene detection and antimicrobial susceptibility tests, a comprehensive treatment regimen (amikacin + sodium bicarbonate + glucose) was designed, and clinical trial indicated that the 3-day cure rate in the treatment group reached 100 % (24/24), significantly higher than 14.3 % (2/14) in the control group. This study revealed a synergistic pathogenic mechanism between <em>Escherichia coli HlyD</em>/<em>K88</em> and <em>Staphylococcus aureus</em> enterotoxins, confirming that an amikacin-based combined treatment regimen can effectively alleviate FKS symptoms, thereby providing a theoretical basis for precision medication in FKS.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105699"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of fungal urinary tract disease in dogs and cats: a scoping review","authors":"J. Scott Weese ,&nbsp;Heather E. Weese","doi":"10.1016/j.rvsc.2025.105710","DOIUrl":"10.1016/j.rvsc.2025.105710","url":null,"abstract":"<div><div>Fungal urinary tract disease has been poorly studied in veterinary medicine. While it is an uncommon condition, identification of fungal cystitis or pyelonephritis raises many clinical questions about optimal management practices. To better understand optimal management approaches and inform guideline development, a scoping review was to identify available evidence pertaining to treatment of fungal urinary tract disease in dogs and cats. After de-duplication and relevance screening, 28 studies were included in the synthesis). Twenty-six (93 %) were single case reports and 2 (7.1 %) were case series' describing fungal infections in 16 (62 %) dogs and 10 (38 %) cats. Antifungal treatment of cystitis, pyelonephritis, funguria and fungal balls was described, with variable drugs and regimens. Varied treatment approaches and outcomes have been reported, but controlled trials are lacking. In lieu of that, larger and more structured multicentre observational studies are needed to better understand treatment approaches and inform evidence-based guidelines.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105710"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of burnout syndrome among veterinarians: An assessment using the Copenhagen Burnout Inventory","authors":"Sofija Šolaja ,&nbsp;Ivan Vićić ,&nbsp;Vladimir Nešić ,&nbsp;Kristina Spariosu ,&nbsp;Dajana Davitkov","doi":"10.1016/j.rvsc.2025.105708","DOIUrl":"10.1016/j.rvsc.2025.105708","url":null,"abstract":"<div><div>This study aimed to investigate the burnout syndrome among Serbian veterinarians across different veterinary fields using the Copenhagen Burnout Inventory (CBI). Moreover, possible risk factors associated with the development of burnout levels in this population were also studied. The explanatory and confirmatory factor analysis revealed two-factor solutions from the three original CBI domains, explaining a total of 65.5 % variance among the investigated veterinarian population. The first factor, which explained 56.3 % of the variance, was predominantly defined by personal burnout, with high loadings ranging from 0.931 to 0.779. The second factor accounted for 9.2 % of the variance and was primarily characterized by client-related burnout (loadings between 0.942 and 0.580). Items from the work-related burnout domain of the original CBI showed cross-loadings on both factors, reflecting its overlap with personal and client-related dimensions. Therefore, personal/work-related and client/work-related domains were identified in the investigated veterinarian population. The common risk factor for two defined types of burnout was a decreased number of vacation days per year, 1–10 days. Working in science had a protective effect on the development of burnout syndrome compared to the small practice. Establishing a protective buffer through a series of activities at the individual and organizational levels, increasing vacation days in our case, could help contain or prevent burnout syndrome among the veterinarian population.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105708"},"PeriodicalIF":2.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Less invasive sperm collection alternatives and implications for reproduction and conservation: A review","authors":"Andreia Nobre Anciuti ,&nbsp;Julia Gonçalves Rodrigues ,&nbsp;Mariana Costa-Santos ,&nbsp;Lucas Moraes Schaidt-Lopes ,&nbsp;Isabella de Moura Folhadella Pires ,&nbsp;Marcos de Lucca Moreira Gomes ,&nbsp;Janaína Barcelos Porto Ferreira","doi":"10.1016/j.rvsc.2025.105707","DOIUrl":"10.1016/j.rvsc.2025.105707","url":null,"abstract":"<div><div>Semen collection from laboratory and wild animals is essential for biomedical research, assisted reproduction programs, and conservation strategies for endangered species. This article presents a critical and comparative review of the main methods available, categorizing them into invasive techniques, traditional <em>in vivo</em> techniques, and less invasive alternatives. In addition to describing the procedures, the study evaluates the efficiency, applicability by species, level of invasiveness, cost, and ethical suitability of each approach. The analysis also integrates the principles of the 3Rs (Reduction, Refinement, and Replacement), highlighting methods that promote animal welfare. Based on recent data, this work provides an updated view on trends and innovations in sperm collection, encouraging the rational and ethical use of these practices in different scientific contexts.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"192 ","pages":"Article 105707"},"PeriodicalIF":2.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}