Research in veterinary science最新文献

{"title":"Productive and welfare parameters in trimmed laying hens: A systematic review-meta-analysis","authors":"Henrique Sonda Gallina ,&nbsp;Maria Eugênia Andrighetto Canozzi ,&nbsp;Pietra Viertel Molinari ,&nbsp;Juahil Martins de Oliveira Júnior ,&nbsp;Ivan Bianchi ,&nbsp;Fabiana Moreira ,&nbsp;Débora Cristina Olsson ,&nbsp;Paula Gabriela da Silva Pires ,&nbsp;Javier Sanchez ,&nbsp;Vanessa Peripolli","doi":"10.1016/j.rvsc.2025.105883","DOIUrl":"10.1016/j.rvsc.2025.105883","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of beak trimming—using a hot blade or infrared radiation—on mortality, egg production, feather pecking, and corticosterone levels in laying hens, using systematic review-meta-analysis (SR-MA) methodology. A total of 1775 citations were identified. The number of birds evaluated was 76 for corticosterone, 6172 for mortality, and 5460 for egg production. Twenty-three articles were assessed, with 13 included in the quantitative synthesis. Birds subjected to hot blade trimming showed a lower probability of mortality (Relative Risk - RR = 0.47; <em>p</em> = 0.087) compared to untrimmed birds, with high heterogeneity (I² = 94.64 %). Both shallow (≤ 5 mm; RR = 0.64; <em>p</em> &lt; 0.0001) and deep (&gt; 5 mm; RR = 0.02; p &lt; 0.0001) beak trimming reduced mortality compared to untrimmed birds. No consistent evidence was found linking beak trimming to changes in egg production or corticosterone levels; however, due to the low number of studies and methodological variability, these findings should be interpreted with caution. Infrared beak trimming appears to be a promising, less invasive alternative, showing potential welfare benefits without compromising performance. Further standardized research is needed to strengthen conclusions, especially on stress-related physiological responses.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105883"},"PeriodicalIF":1.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MHC compatibility influences the interaction between different types of equine mesenchymal stem/stromal cells and the local immune response","authors":"Alina Cequier ,&nbsp;Mª. Belén Serrano ,&nbsp;Mª. Teresa Soler-Monsó ,&nbsp;Elvira Bernad ,&nbsp;Francisco José Vázquez ,&nbsp;Arantza Vitoria ,&nbsp;Sara Fuente ,&nbsp;Pilar Zaragoza ,&nbsp;Antonio Romero ,&nbsp;Clementina Rodellar ,&nbsp;Laura Barrachina","doi":"10.1016/j.rvsc.2025.105889","DOIUrl":"10.1016/j.rvsc.2025.105889","url":null,"abstract":"<div><div>The allogeneic administration of equine mesenchymal stem/stromal cells (MSCs) has numerous advantages over autologous therapy, but their interactions with the patient's immune system need to be further elucidated. These interactions can be influenced by factors such as the compatibility between donor-receptor for the major histocompatibility complex (MHC) and by the MHC expression levels, which can change under different conditions like inflammatory exposure and chondrogeneic differentiation. In this study, we evaluated the local immune response induced by chondrogeneically differentiated (MSC-chondro), pro-inflammatory primed (MSC-primed) and basal (MSC-naïve) MSCs, and how this response changes the immunomodulatory and immunogenic profiles of MSCs <em>in vivo</em>. Equine MSCs were embedded in alginate scaffolds and subcutaneously implanted into autologous, MHC-matched and MHC-mismatched horses. Scaffolds were recovered at different time-points for histologic and gene expression analyses, and the procedure was repeated to assess the effect of a second administration. Our results suggest that MHC-compatibility may play a key role in attenuating the local immune response induced by MSCs, which may be related to the upregulation of immunomodulatory genes in the three MSC types <em>in vivo</em>. In contrast, when MSCs were administered into MHC-mismatched horses, expression of immunogenic genes was higher across all MSC conditions. Therefore, the conditions in which MSCs are administered may not affect the long-term local immune response, but MHC-matched administration would favour the immune evasion of MSCs, thus being advisable especially when repeated MSC administrations are required. Comprehensively investigating the <em>in vivo</em> immune response against equine allogeneic MSCs is crucial for advancing veterinary cell therapies.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105889"},"PeriodicalIF":1.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selected comparative aspects and unexpected findings in acute phase proteins and other biomarkers of animal health and welfare","authors":"P.D. Eckersall ,&nbsp;J.J. Ceron","doi":"10.1016/j.rvsc.2025.105870","DOIUrl":"10.1016/j.rvsc.2025.105870","url":null,"abstract":"<div><div>Recent years have seen advances in clinical biochemistry of domestic animals which have highlighted comparative differences between species and have also identified fundamental aspects of the biochemical mechanisms in physiological conditions and disease, that have implications across species, including human, health and welfare. From investigations in diverse species using biochemical, immunological, proteomic and metabolomic approaches a series of species particularities and unexpected results for some biomarkers have been made. These observations cover (1) the differences between species in the acute phase protein (APP) response to infection and inflammation; (2) the non-hepatic synthesis and release in the mammary gland, adipose tissue and intestine of APP (3) the response of haptoglobin (HP) as a biomarker for stress; (4) observations in non-mammalian species related to hemopexin and HP; (5) the response of bile acids in milk to mastitis; (6) barley serine protease inhibitors being identified in bovine faeces; (7) alkaline phosphatase being present in bovine nasal secretion; (8) saliva findings with analytes such as adenine deaminase showing different activity between saliva and serum and a detergent-like surfactant protein, latherin being found in equine saliva and sweat and (9) serum enzymes and selective muscle protein reaction of Atlantic salmon as an example of the differences in biochemistry between terrestrial and aquatic species.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105870"},"PeriodicalIF":1.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of Listeria spp. and Listeria monocytogenes in half-carcasses, meat cuts, equipment, and the environment of bovine slaughterhouses in Brazil","authors":"Leonardo França ,&nbsp;Ursula Nunes Rauecker ,&nbsp;Daniel Lucino Silva dos Santos ,&nbsp;Naara Aparecida Almeida ,&nbsp;Clarice Gebara ,&nbsp;Cristiano Sales Prado ,&nbsp;Valéria Quintana Cavicchioli ,&nbsp;Moacir Evandro Lage ,&nbsp;Francine Oliveira Duarte ,&nbsp;Cíntia Silva Minafra e Rezende ,&nbsp;Ana Maria de Souza Almeida ,&nbsp;Iolanda Aparecida Nunes","doi":"10.1016/j.rvsc.2025.105869","DOIUrl":"10.1016/j.rvsc.2025.105869","url":null,"abstract":"<div><div>Human listeriosis is a severe food-borne illness, with fatality rates ranging from 20 to 30 %. In Brazil, despite being an underdiagnosed and underreported disease, the presence of the microorganism in food has been the subject of important studies. However, its occurrence in slaughterhouse environments has received little attention in recent years. Therefore, the aim of this study was to determine the occurrence of <em>Listeria</em> spp. and <em>L. monocytogenes</em> in samples of environments (boning rooms, cold rooms, and slaughter rooms), equipment, half-carcasses, and retail cuts. Samples were collected from 25 slaughterhouse units belonging to five industries under the Federal Inspection Service (SIF) licensed for international trade and located in Brazilian states (São Paulo, Mato Grosso do Sul, Goiás, Mato Grosso, Bahia, Pará, and Minas Gerais), using Polymerase Chain Reaction (PCR). Primers pairs U1/LI1 for <em>Listeria</em> spp., and LM1/LM2 and LL5/LL6 (Invitrogen©) for L. <em>monocytogenes</em> were used for amplification. <em>Listeria</em> spp. was detected in 19.7 % (71/360) of the total samples analyzed, with detection rates of 23 % in deboning rooms, cold rooms, and half-carcasses, 15 % in meat cuts, and 10 % in slaughter rooms. In the total analyzed, <em>L. monocytogenes</em> was detected in 14.7 % (53/360) of the samples, with rates of 20 % in cold rooms, 15.8 % in boning rooms, 13.3 % in half-carcasses and meat cuts, and 10 % in slaughter rooms. The LM1/LM2 primer pair proved more efficient than LL5/LL6. The results of this study highlight the need for urgent measures to control the pathogen in cattle slaughterhouses in Brazil.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"196 ","pages":"Article 105869"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid screening method for detection of ampicillin residues in dairy milk","authors":"Nida Noreen ,&nbsp;Bushra Akhtar ,&nbsp;Chi-Chung Chou ,&nbsp;Muhammad Irfan Anwar ,&nbsp;Mian Muhammad Awais ,&nbsp;Faqir Muhammad","doi":"10.1016/j.rvsc.2025.105867","DOIUrl":"10.1016/j.rvsc.2025.105867","url":null,"abstract":"<div><div>The extended use of antibiotics in dairy animals for the growth promotion and disease prevention is directly linked with the development of antimicrobial resistance. This study reports the development of a quick method for the detection of ampicillin residues in dairy milk, using the principle of Lateral Flow Assay (LFA). In this study, hapten against ampicillin was prepared by conjugation with bovine serum albumin. Primary antibodies against this hapten were raised in rabbits and purified. Total protein (IgG) in hyperimmune serum was measured and evaluated by direct ELISA. Gold nanoparticles were conjugated with primary antibodies and impregnated on the conjugate pad of a multi-layered residue detection strip, placed in plastic cassette with clear demarcations of test and control lines on nitrocellulose membrane. The LFA developed using polyclonal antibodies demonstrated the rapid detection of maximum residue limits (MRL) of ampicillin (5 ppb) in milk samples within 8 to 10 min. The kit developed in this study can be used for screening ampicillin residues in milk samples throughout the supply chain, starting from production to consumers' doorstep.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105867"},"PeriodicalIF":1.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High genetic variability in Taenia multiceps larvae: A mitochondrial DNA-based study of sheep isolates using CO1 and NADH1 genes","authors":"Figen Celik ,&nbsp;Muhammet Uslug ,&nbsp;Afra Sena Tekin ,&nbsp;Sami Simsek","doi":"10.1016/j.rvsc.2025.105863","DOIUrl":"10.1016/j.rvsc.2025.105863","url":null,"abstract":"<div><div><em>Coenurus cerebralis</em>, the larval stage of <em>Taenia multiceps</em>, is a significant parasitic threat to small ruminants, yet its molecular diversity remains underexplored in many endemic regions. This study investigated the genetic variability of <em>C. cerebralis</em> from sheep in Elazig province, Türkiye, by analyzing mitochondrial CO1 (812 bp) and NADH1 (498 bp) gene regions. Sequence data from 39 isolates confirmed the presence of <em>T. multiceps</em>, with 21 CO1 and 17 NADH1 haplotypes identified over half of which were singletons. Most polymorphic sites were parsimony-informative, indicating meaningful evolutionary divergence within the population. Neutrality tests (Tajima's D and Fu's Fs) suggested signals of population expansion or selection pressure. Phylogenetic analyses confirmed the genetic distinctiveness of the isolates and revealed higher diversity than previously documented in Türkiye. The observed haplotype diversity, coupled with low nucleotide diversity, reflects recent diversification events within the parasite population. These findings highlight ongoing microevolutionary processes in <em>C. cerebralis</em> and underscore the need for region-specific molecular surveillance and targeted control measures. Enhanced understanding of genetic variation in <em>C. cerebralis</em> is critical for improving diagnostic strategies, informing regional epidemiological models, and designing effective intervention programs against coenurosis in endemic areas.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105863"},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized two-step gradient PCR for efficient clinical screening of vector-borne hemoparasites in goats","authors":"Sasi Adithya ,&nbsp;Y. Ajith ,&nbsp;Varuna P. Panicker ,&nbsp;K.S. Athira ,&nbsp;N. Athira ,&nbsp;K. Athira ,&nbsp;P. Preena ,&nbsp;P.V. Tresamol","doi":"10.1016/j.rvsc.2025.105865","DOIUrl":"10.1016/j.rvsc.2025.105865","url":null,"abstract":"<div><div>Vector-borne pathogens pose a significant threat to the optimal production performance of farm animals in tropical endemic regions like South India. Early clinical diagnosis and timely administration of targeted chemotherapy are crucial for developing effective control strategies against these vector-borne diseases (VBDs). Although arthropod vectors transmit various pathogens, the major VBDs affecting goats can be categorized into hemotropic bacteria (<em>Anaplasma/Ehrlichia</em> and <em>Mycoplasma</em> spp.) and hemoprotozoans (<em>Theileria</em>, <em>Babesia</em>, <em>Hepatozoon</em>, and <em>Trypanosoma</em> spp.). The simultaneous detection of these six genera typically requires multiple PCR protocols with entirely different thermal conditions, and attempts to standardize multiplex PCR often fail due to this variation. We developed an optimized screening method using the gradient function of a PCR thermocycler, enabling simultaneous genus-specific detection. Blood DNA was extracted, and validated primers from the literature were applied in a two-step process: initial screening with universal primers (16S, 18S, apicomplexan rRNA), followed by genus-specific PCR. The gradient function of the thermocycler was employed in both steps to accommodate varying annealing temperatures, enabling simultaneous amplification of target sequences from different genera within the same PCR run. This approach reduces testing time, enhances detection in clinical samples, and facilitates precise, narrow-spectrum therapy, thereby minimizing treatment failures and mitigating the risk of drug resistance. This protocol provides a rapid and broadly adaptable diagnostic tool for the simultaneous detection of multiple hemoparasite genera, supporting timely, targeted treatment and improved management of VBDs in clinical settings.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105865"},"PeriodicalIF":1.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of hemotropic mycoplasmas in cats and dogs from northern Vietnam: Evidence of interspecies transmission of Mycoplasma suis-like and Mycoplasma haemocanis-like in cats","authors":"Thuong Thi Huyen Bui ,&nbsp;Kritsada Thongmeesee ,&nbsp;Wittawat Wechtaisong ,&nbsp;Khanh Linh Bui ,&nbsp;Sonthaya Tiawsirisup","doi":"10.1016/j.rvsc.2025.105866","DOIUrl":"10.1016/j.rvsc.2025.105866","url":null,"abstract":"<div><div>Hemoplasmas are bacteria of the genus <em>Mycoplasma</em> that can attach to red blood cells and cause hemolytic anemia. The two main species that can infect dogs are <em>Mycoplasma</em> (<em>M.</em>) <em>haemocanis</em> and ‘<em>Candidatus</em> (<em>Ca.</em>) M. haematoparvum’; and three major species that can infect cats are <em>M. haemofelis</em>, ‘<em>Ca.</em> M. haemominutum’, and ‘<em>Ca.</em> M. turicensis’. However, there is a lack of research data regarding hemoplasma species infecting dogs and cats in Vietnam. Therefore, we aimed to investigate the molecular presence of hemotropic <em>Mycoplasma</em> in dogs and cats in northern Vietnam. We collected blood samples from dogs and cats and used PCR to precisely target the 16S rRNA gene of hemoplasma species in the blood samples. The results indicated that 1.2 % (95 % CI: 0.5 %–2.7 %; 6/489) of all samples, 1.4 % of cat samples (95 % CI: 0.4 %–3.5 %; 4/288) and 1 % of dog samples (95 % CI: 0.1 %–3.5 %; 2/201) were infected with hemoplasmas. Phylogenetic analysis showed the presence of two different species of hemoplasma, namely <em>M. haemocanis</em>-like and <em>M. suis</em>-likeg. Additional genetic characterization based on the 23S rRNA and RNase P RNA (<em>rnpB</em>) genes indicated that <em>M. haemocanis</em>-like isolates could be <em>M. haemocanis</em>, rather than <em>M. haemofelis</em>. Moreover, we detected <em>M. haemocanis</em>-like and <em>M. suis</em>-like in the blood samples collected from cats, indicating the possibility of interspecies transmission. Although previous studies have detected hemoplasma species in Vietnam, to the best of our knowledge, this study is the first to report the presence of <em>M. haemocanis</em>-like and <em>M. suis</em>-like in cats and <em>M. haemocanis</em>-like in dogs from Vietnam.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105866"},"PeriodicalIF":1.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of lytic bacteriophage vB_EhoP_ZX13 and its therapeutic potential against Enterobacter hormaechei infection","authors":"Yilin Liu ,&nbsp;Shuang Liang ,&nbsp;Jincheng Zhang ,&nbsp;Xinyi Cai ,&nbsp;Yue Sun ,&nbsp;Cuixing Zhan ,&nbsp;Xin Zhou","doi":"10.1016/j.rvsc.2025.105864","DOIUrl":"10.1016/j.rvsc.2025.105864","url":null,"abstract":"<div><div><em>Enterobacter hormaechei</em> (<em>E. hormaechei</em>) is an opportunistic pathogen that can cause various animal diseases under certain conditions, including pneumonia, peritonitis, and pyogenic endometritis. The emergence of multidrug-resistant <em>E. hormaechei</em> has severely limited treatment options, highlighting the urgent need for alternative therapeutic strategies such as bacteriophage therapy. In this study, we isolated and characterized a lytic bacteriophage, vB_EhoP_ZX13, from aquaculture samples using <em>E. hormaechei</em>-Eho13 as the host. Morphological analysis revealed that vB_EhoP_ZX13 exhibited typical long-tailed phage characteristics and belonged to the family <em>Straboviridae</em>. It demonstrated the optimal infection efficiency at a multiplicity of infection (MOI) of 1, with a short latent period (5 min) and a high burst size (100 PFU/cell). Furthermore, the phage remained stable at 4–30 °C and pH 5–8, but was sensitive to high temperatures and ultraviolet (UV) light exposure. Genomic analysis revealed a 172,900 bp double-stranded DNA (dsDNA) genome encoding 295 open reading frames (ORFs) and 19 tRNAs. Crucially, no genes associated with virulence factors, lysogenic genes, or antibiotic resistance-related were identified, supporting its biosafety for therapeutic. In vitro, vB_EhoP_ZX13 significantly inhibited the growth of Eho13. In vivo, phage treatment markedly improved the survival rate of infected mice, with a 90 % survival observed when administered at an MOI of 1. In summary, vB_EhoP_ZX13 is a lytic phage with strong antibacterial activity both in vitro and in vivo, highlighting its promise as an alternative therapeutic agent against <em>E. hormaechei</em> infections.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105864"},"PeriodicalIF":1.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative analysis, clinical-laboratory parameters, and quality of life in treatment associated with ozone therapy in female dogs with mammary tumors","authors":"Viviane Abreu Pedreira de Oliveira ,&nbsp;Marcela Próspero Rodrigues Pinto ,&nbsp;Daniela Farias Larangeira ,&nbsp;Laís Pereira Silva ,&nbsp;Vitor de Moraes Pina de Carvalho ,&nbsp;Gisele André Baptista Canuto ,&nbsp;Hanna Carvalho de Sá ,&nbsp;Gabriel Rodrigues Menezes ,&nbsp;Karine Araújo Damasceno ,&nbsp;Stella Maria Barrouin-Melo ,&nbsp;Alessandra Estrela-Lima","doi":"10.1016/j.rvsc.2025.105839","DOIUrl":"10.1016/j.rvsc.2025.105839","url":null,"abstract":"<div><div>Canine mammary tumors (CMT) primarily affect female dogs. Their high prevalence, chronic nature, and potential aggressiveness often lead to metastasis, systemic disease, and death despite the use of conventional treatment based on surgery and chemotherapy. There is a need for complementary, accessible, and low-cost therapies to improve the perioperative care of these canine patients. In this context, this study aimed to evaluate the effectiveness of ozonated autohemotherapy as an integrative therapy by analyzing markers of oxidative balance, clinical-laboratory parameters, and quality of life of dogs with CMT. Forty-nine dogs were examined and allocated into study groups: dogs treated with only mastectomy (GM; <em>n</em> = 11), mastectomy with ozonated autohemotherapy (GM-AHmO₃; <em>n</em> = 13), and mastectomy with non-ozonated autohemotherapy (GM-AHm; <em>n</em> = 12); a fourth group contained negative control healthy dogs (GH; n = 13). Assessment of clinical, hematological (erythrocytes, platelets, lymphocytes, urea, creatinine, ALT, ALP), oxidative markers (TAC and MDA), and quality-of-life was performed at five-day points, D0, D1, D2, D3, and D4. The GM-AHmO₃ showed progressive improvement in quality-of-life scores (from 26.4 to 32; <em>p</em> &lt; 0.001), increased total antioxidant capacity (TAC: 13.1 to 16.1; <em>p</em> = 0.003), and decreased malondialdehyde levels (MDA: 438 to 150.5; <em>p</em> = 0.002) from D0 to D4. Significant reductions were observed in creatinine (<em>p</em> = 0.006), ALT (<em>p</em> = 0.001), and ALP (<em>p</em> = 0.043) in GM-AHmO₃, indicating improvement in renal and hepatic profiles. Additionally, a significant decrease in creatinine levels was noted at D4 in GM-AHmO₃ (<em>p</em> = 0.017). Comparative data between groups and time points showed that ozonated autohemotherapy enhanced antioxidant responses, reduced oxidative stress, improved renal and hepatic functions, and, importantly, improved CMT patients' life quality. As a preliminary investigation, this study indicates that AHmO₃ is a beneficial approach as a complementary method to enhance the well-being of dogs with CMTs undergoing mastectomy.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105839"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}