越南北部猫和狗中嗜血支原体的分子检测：猫中瑞士样支原体和嗜血病样支原体种间传播的证据

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-22 DOI:10.1016/j.rvsc.2025.105866

Thuong Thi Huyen Bui , Kritsada Thongmeesee , Wittawat Wechtaisong , Khanh Linh Bui , Sonthaya Tiawsirisup

{"title":"越南北部猫和狗中嗜血支原体的分子检测：猫中瑞士样支原体和嗜血病样支原体种间传播的证据","authors":"Thuong Thi Huyen Bui , Kritsada Thongmeesee , Wittawat Wechtaisong , Khanh Linh Bui , Sonthaya Tiawsirisup","doi":"10.1016/j.rvsc.2025.105866","DOIUrl":null,"url":null,"abstract":"<div><div>Hemoplasmas are bacteria of the genus Mycoplasma that can attach to red blood cells and cause hemolytic anemia. The two main species that can infect dogs are Mycoplasma (M.) haemocanis and ‘Candidatus (Ca.) M. haematoparvum’; and three major species that can infect cats are M. haemofelis, ‘Ca. M. haemominutum’, and ‘Ca. M. turicensis’. However, there is a lack of research data regarding hemoplasma species infecting dogs and cats in Vietnam. Therefore, we aimed to investigate the molecular presence of hemotropic Mycoplasma in dogs and cats in northern Vietnam. We collected blood samples from dogs and cats and used PCR to precisely target the 16S rRNA gene of hemoplasma species in the blood samples. The results indicated that 1.2 % (95 % CI: 0.5 %–2.7 %; 6/489) of all samples, 1.4 % of cat samples (95 % CI: 0.4 %–3.5 %; 4/288) and 1 % of dog samples (95 % CI: 0.1 %–3.5 %; 2/201) were infected with hemoplasmas. Phylogenetic analysis showed the presence of two different species of hemoplasma, namely M. haemocanis-like and M. suis-likeg. Additional genetic characterization based on the 23S rRNA and RNase P RNA (rnpB) genes indicated that M. haemocanis-like isolates could be M. haemocanis, rather than M. haemofelis. Moreover, we detected M. haemocanis-like and M. suis-like in the blood samples collected from cats, indicating the possibility of interspecies transmission. Although previous studies have detected hemoplasma species in Vietnam, to the best of our knowledge, this study is the first to report the presence of M. haemocanis-like and M. suis-like in cats and M. haemocanis-like in dogs from Vietnam.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"195 ","pages":"Article 105866"},"PeriodicalIF":1.8000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular detection of hemotropic mycoplasmas in cats and dogs from northern Vietnam: Evidence of interspecies transmission of Mycoplasma suis-like and Mycoplasma haemocanis-like in cats\",\"authors\":\"Thuong Thi Huyen Bui , Kritsada Thongmeesee , Wittawat Wechtaisong , Khanh Linh Bui , Sonthaya Tiawsirisup\",\"doi\":\"10.1016/j.rvsc.2025.105866\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Hemoplasmas are bacteria of the genus Mycoplasma that can attach to red blood cells and cause hemolytic anemia. The two main species that can infect dogs are Mycoplasma (M.) haemocanis and ‘Candidatus (Ca.) M. haematoparvum’; and three major species that can infect cats are M. haemofelis, ‘Ca. M. haemominutum’, and ‘Ca. M. turicensis’. However, there is a lack of research data regarding hemoplasma species infecting dogs and cats in Vietnam. Therefore, we aimed to investigate the molecular presence of hemotropic Mycoplasma in dogs and cats in northern Vietnam. We collected blood samples from dogs and cats and used PCR to precisely target the 16S rRNA gene of hemoplasma species in the blood samples. The results indicated that 1.2 % (95 % CI: 0.5 %–2.7 %; 6/489) of all samples, 1.4 % of cat samples (95 % CI: 0.4 %–3.5 %; 4/288) and 1 % of dog samples (95 % CI: 0.1 %–3.5 %; 2/201) were infected with hemoplasmas. Phylogenetic analysis showed the presence of two different species of hemoplasma, namely M. haemocanis-like and M. suis-likeg. Additional genetic characterization based on the 23S rRNA and RNase P RNA (rnpB) genes indicated that M. haemocanis-like isolates could be M. haemocanis, rather than M. haemofelis. Moreover, we detected M. haemocanis-like and M. suis-like in the blood samples collected from cats, indicating the possibility of interspecies transmission. Although previous studies have detected hemoplasma species in Vietnam, to the best of our knowledge, this study is the first to report the presence of M. haemocanis-like and M. suis-like in cats and M. haemocanis-like in dogs from Vietnam.</div></div>\",\"PeriodicalId\":21083,\"journal\":{\"name\":\"Research in veterinary science\",\"volume\":\"195 \",\"pages\":\"Article 105866\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in veterinary science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0034528825003406\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in veterinary science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0034528825003406","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

血浆是支原体属的细菌，可以附着在红细胞上并引起溶血性贫血。可以感染狗的两个主要物种是血支原体和血支原体；可以感染猫的三个主要物种是血支原体、Ca。M. haemominutum”和“Ca。m . turicensis”。然而，缺乏关于越南狗和猫感染血浆种类的研究数据。因此，我们的目的是调查在越南北部的狗和猫嗜血支原体的分子存在。我们采集了狗和猫的血液样本，利用PCR技术精确定位血液样本中血浆种类的16S rRNA基因。结果表明，所有样本中有1.2% （95% CI: 0.5% - 2.7%; 6/489）、1.4%的猫样本（95% CI: 0.4% - 3.5%; 4/288）和1%的狗样本（95% CI: 0.1% - 3.5%; 2/201）感染了血浆。系统发育分析显示存在两种不同的血浆，即类风湿血原体和瑞士类风湿血原体。另外，基于23S rRNA和RNase P RNA （rnpB）基因的遗传特征表明，嗜血杆菌样分离物可能是嗜血杆菌，而不是嗜血杆菌。此外，我们在收集的猫的血液样本中检测到血蜱样支原体和瑞士支原体样支原体，表明可能存在种间传播。虽然以前的研究已经在越南发现了血原体种类，但据我们所知，这项研究是第一次报告在越南的猫和狗中存在血原体样和瑞士样。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Molecular detection of hemotropic mycoplasmas in cats and dogs from northern Vietnam: Evidence of interspecies transmission of Mycoplasma suis-like and Mycoplasma haemocanis-like in cats

查看原文本刊更多论文

Molecular detection of hemotropic mycoplasmas in cats and dogs from northern Vietnam: Evidence of interspecies transmission of Mycoplasma suis-like and Mycoplasma haemocanis-like in cats

Hemoplasmas are bacteria of the genus Mycoplasma that can attach to red blood cells and cause hemolytic anemia. The two main species that can infect dogs are Mycoplasma (M.) haemocanis and ‘Candidatus (Ca.) M. haematoparvum’; and three major species that can infect cats are M. haemofelis, ‘Ca. M. haemominutum’, and ‘Ca. M. turicensis’. However, there is a lack of research data regarding hemoplasma species infecting dogs and cats in Vietnam. Therefore, we aimed to investigate the molecular presence of hemotropic Mycoplasma in dogs and cats in northern Vietnam. We collected blood samples from dogs and cats and used PCR to precisely target the 16S rRNA gene of hemoplasma species in the blood samples. The results indicated that 1.2 % (95 % CI: 0.5 %–2.7 %; 6/489) of all samples, 1.4 % of cat samples (95 % CI: 0.4 %–3.5 %; 4/288) and 1 % of dog samples (95 % CI: 0.1 %–3.5 %; 2/201) were infected with hemoplasmas. Phylogenetic analysis showed the presence of two different species of hemoplasma, namely M. haemocanis-like and M. suis-likeg. Additional genetic characterization based on the 23S rRNA and RNase P RNA (rnpB) genes indicated that M. haemocanis-like isolates could be M. haemocanis, rather than M. haemofelis. Moreover, we detected M. haemocanis-like and M. suis-like in the blood samples collected from cats, indicating the possibility of interspecies transmission. Although previous studies have detected hemoplasma species in Vietnam, to the best of our knowledge, this study is the first to report the presence of M. haemocanis-like and M. suis-like in cats and M. haemocanis-like in dogs from Vietnam.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.