Oxidative analysis, clinical-laboratory parameters, and quality of life in treatment associated with ozone therapy in female dogs with mammary tumors

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-20 DOI:10.1016/j.rvsc.2025.105839

Viviane Abreu Pedreira de Oliveira , Marcela Próspero Rodrigues Pinto , Daniela Farias Larangeira , Laís Pereira Silva , Vitor de Moraes Pina de Carvalho , Gisele André Baptista Canuto , Hanna Carvalho de Sá , Gabriel Rodrigues Menezes , Karine Araújo Damasceno , Stella Maria Barrouin-Melo , Alessandra Estrela-Lima

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引用次数: 0

Abstract

Canine mammary tumors (CMT) primarily affect female dogs. Their high prevalence, chronic nature, and potential aggressiveness often lead to metastasis, systemic disease, and death despite the use of conventional treatment based on surgery and chemotherapy. There is a need for complementary, accessible, and low-cost therapies to improve the perioperative care of these canine patients. In this context, this study aimed to evaluate the effectiveness of ozonated autohemotherapy as an integrative therapy by analyzing markers of oxidative balance, clinical-laboratory parameters, and quality of life of dogs with CMT. Forty-nine dogs were examined and allocated into study groups: dogs treated with only mastectomy (GM; n = 11), mastectomy with ozonated autohemotherapy (GM-AHmO₃; n = 13), and mastectomy with non-ozonated autohemotherapy (GM-AHm; n = 12); a fourth group contained negative control healthy dogs (GH; n = 13). Assessment of clinical, hematological (erythrocytes, platelets, lymphocytes, urea, creatinine, ALT, ALP), oxidative markers (TAC and MDA), and quality-of-life was performed at five-day points, D0, D1, D2, D3, and D4. The GM-AHmO₃ showed progressive improvement in quality-of-life scores (from 26.4 to 32; p < 0.001), increased total antioxidant capacity (TAC: 13.1 to 16.1; p = 0.003), and decreased malondialdehyde levels (MDA: 438 to 150.5; p = 0.002) from D0 to D4. Significant reductions were observed in creatinine (p = 0.006), ALT (p = 0.001), and ALP (p = 0.043) in GM-AHmO₃, indicating improvement in renal and hepatic profiles. Additionally, a significant decrease in creatinine levels was noted at D4 in GM-AHmO₃ (p = 0.017). Comparative data between groups and time points showed that ozonated autohemotherapy enhanced antioxidant responses, reduced oxidative stress, improved renal and hepatic functions, and, importantly, improved CMT patients' life quality. As a preliminary investigation, this study indicates that AHmO₃ is a beneficial approach as a complementary method to enhance the well-being of dogs with CMTs undergoing mastectomy.

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氧化分析，临床-实验室参数，与臭氧治疗相关的雌性犬乳腺肿瘤的生活质量

犬乳腺肿瘤（CMT）主要影响雌性犬。尽管采用基于手术和化疗的常规治疗，但其高患病率、慢性性质和潜在的侵袭性常导致转移、全身性疾病和死亡。需要一种互补性的、可获得的、低成本的治疗方法来改善这些犬类患者的围手术期护理。在此背景下，本研究旨在通过分析氧化平衡指标、临床-实验室参数和CMT犬的生活质量来评估臭氧化自体血液治疗作为一种综合治疗的有效性。49只狗被检查并分配到研究组：只接受乳房切除术（GM, n = 11），乳房切除术联合臭氧化自体血液治疗（GM- ahmo3, n = 13），乳房切除术联合非臭氧化自体血液治疗（GM- ahm, n = 12）；第四组为阴性对照健康犬（GH, n = 13）。评估临床、血液学（红细胞、血小板、淋巴细胞、尿素、肌酐、ALT、ALP）、氧化标志物（TAC和MDA）和生活质量在5天点，D0、D1、D2、D3和D4。GM-AHmO3显示出生活质量评分的渐进式改善（从26.4到32；p < 0.001），总抗氧化能力的增加（TAC: 13.1到16.1;p = 0.003），丙二醛水平从0到4降低（MDA: 438到150.5;p = 0.002）。GM-AHmO3组肌酐（p = 0.006）、ALT （p = 0.001）和ALP （p = 0.043）显著降低，表明肾脏和肝脏状况改善。此外，GM-AHmO3 D4时肌酐水平显著降低（p = 0.017）。各组和时间点之间的比较数据显示，臭氧化自体血液治疗增强了抗氧化反应，降低了氧化应激，改善了肾功能和肝功能，更重要的是，改善了CMT患者的生活质量。作为一项初步研究，本研究表明AHmO3是一种有益的方法，作为一种补充方法，可以提高CMTs接受乳房切除术的狗的健康状况。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.