乳中氨苄西林残留量的快速筛选方法

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-26 DOI:10.1016/j.rvsc.2025.105867

Nida Noreen , Bushra Akhtar , Chi-Chung Chou , Muhammad Irfan Anwar , Mian Muhammad Awais , Faqir Muhammad

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引用次数: 0

摘要

为了促进生长和预防疾病而在奶牛中广泛使用抗生素与抗菌素耐药性的发展直接相关。本研究报告了一种快速检测牛奶中氨苄西林残留的方法，该方法采用横向流动测定法（LFA）的原理。本研究用牛血清白蛋白偶联法制备了抗氨苄西林半抗原。在家兔中培养并纯化了该半抗原的一抗。采用直接ELISA法测定高免疫血清总蛋白（IgG）水平。将金纳米颗粒与一抗偶联，浸渍在多层残留检测条的偶联垫上，放置在硝化纤维素膜上有明确测试线和控制线界限的塑料盒中。利用多克隆抗体建立的LFA可在8 ~ 10 min内快速检测出氨苄西林（5 ppb）在牛奶样品中的最大残留限量（MRL）。本研究开发的试剂盒可用于筛选从生产到消费者家门口整个供应链中牛奶样品中的氨苄西林残留物。

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A rapid screening method for detection of ampicillin residues in dairy milk

The extended use of antibiotics in dairy animals for the growth promotion and disease prevention is directly linked with the development of antimicrobial resistance. This study reports the development of a quick method for the detection of ampicillin residues in dairy milk, using the principle of Lateral Flow Assay (LFA). In this study, hapten against ampicillin was prepared by conjugation with bovine serum albumin. Primary antibodies against this hapten were raised in rabbits and purified. Total protein (IgG) in hyperimmune serum was measured and evaluated by direct ELISA. Gold nanoparticles were conjugated with primary antibodies and impregnated on the conjugate pad of a multi-layered residue detection strip, placed in plastic cassette with clear demarcations of test and control lines on nitrocellulose membrane. The LFA developed using polyclonal antibodies demonstrated the rapid detection of maximum residue limits (MRL) of ampicillin (5 ppb) in milk samples within 8 to 10 min. The kit developed in this study can be used for screening ampicillin residues in milk samples throughout the supply chain, starting from production to consumers' doorstep.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.