Ligilactobacillus salivarius and Limosilactobacillus reuteri improve growth and intestinal health in broilers via modulating gut microbiota and immune response

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-07-30 DOI:10.1016/j.rvsc.2025.105837

Neha Chavan , Mahesh Gupta , Krushna Bibichand Bahiram , Jayant Pandurang Korde , Mukund Kadam , Atul Dhok , Sachin Kumar

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引用次数: 0

Abstract

The present study evaluated the effects of Ligilactobacillus salivarius RBL50, Limosilactobacillus reuteri SW23, and their mixture on growth, gut health, gut microbes, immune response, and serum lipids in broiler chickens. A total of 180 one-day-old broiler chicks were divided into five groups for six weeks: control, antibiotic (BMD), L. salivarius RBL50, L. reuteri SW23, and a combination of L. salivarius RBL50 + L. reuteri SW23. The results showed that probiotics and antibiotic groups had significantly higher average body weight (ABW) and average daily gain (ADG) compared to the control. Probiotics and antibiotics also reduced feed conversion ratio (FCR) and feed intake (ADFI), with the L. salivarius RBL50 + L. reuteri SW23 group showing the best performance. Histomorphological analysis revealed increased villus height (VH) and villus height/crypt depth ratio in the jejunum of probiotic and antibiotic groups, with RBL50 showing the highest values. Broilers treated with probiotic bacteria has higher expression of claudin-1, ZO-1, reduced total viable and coliform counts, and increased Lactobacillus counts in the cecum. Response to intradermal injection of Phyto-haemagglutinin-P (PHAP) was significantly higher in the L. salivarius RBL50 and L. salivarius RBL50 + L. reuteri SW23 groups, but no differences were observed in humoral immune response. Supplementation of L. salivarius RBL 50 and L. reuteri SW23 also increased expression of anti-inflammatory cytokine interleukin-10 (IL-10) in the jejunum and spleen. In conclusion, L. salivarius RBL50 and L. reuteri SW23 can improve growth, gut health, and immune response, making them effective and safe alternatives to antibiotic growth promoters.

查看原文本刊更多论文

唾液乳酸杆菌和罗伊氏乳酸杆菌通过调节肠道菌群和免疫反应促进肉鸡生长和肠道健康

本研究评价了唾液乳酸杆菌RBL50、罗伊氏乳酸杆菌SW23及其混合物对肉鸡生长、肠道健康、肠道微生物、免疫反应和血脂的影响。将180只1日龄肉鸡分为5组，分别为对照组、抗生素组、唾液乳杆菌RBL50组、罗伊氏乳杆菌SW23组和唾液乳杆菌RBL50 +罗伊氏乳杆菌SW23组，试验期6周。结果表明，益生菌组和抗生素组的平均体重（ABW）和平均日增重（ADG）显著高于对照组。益生菌和抗生素均可降低饲料系数（FCR）和采食量（ADFI），其中唾液乳杆菌RBL50 +罗伊氏乳杆菌SW23组表现最佳。组织形态学分析显示，益生菌组和抗生素组空肠绒毛高度（VH）和绒毛高度/隐窝深度比均升高，其中RBL50最高。经益生菌处理的肉鸡盲肠中claudin-1、ZO-1表达升高，总活菌数和大肠菌群数量降低，乳酸杆菌数量增加。唾液乳杆菌RBL50组和唾液乳杆菌RBL50 +罗伊氏乳杆菌SW23组对皮内注射植物血凝素- p （PHAP）的应答显著升高，但体液免疫应答无差异。添加唾液乳杆菌RBL 50和罗伊氏乳杆菌SW23也增加了空肠和脾脏中抗炎细胞因子白细胞介素-10 （IL-10）的表达。综上所述，唾液乳杆菌RBL50和罗伊氏乳杆菌SW23可促进生长、改善肠道健康和免疫反应，是抗生素生长促进剂的安全有效替代品。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.