Reproductive Biology and Endocrinology最新文献

{"title":"Effect of pre-conception serum vitamin D level on pregnancy outcomes in women undergoing in vitro fertilization with fresh embryo transfer: a retrospective analysis.","authors":"Aaron H Y Chan, Jennifer K Y Ko, Raymond H W Li, Tat On Chan, Ernest H Y Ng","doi":"10.1186/s12958-025-01343-w","DOIUrl":"10.1186/s12958-025-01343-w","url":null,"abstract":"<p><strong>Background: </strong>This study aims to investigate the association of pre-conception vitamin D levels on adverse pregnancy outcomes in women undergoing in vitro fertilization with fresh embryo transfer.</p><p><strong>Methods: </strong>This was a retrospective cohort study using archived serum 25-hydroxyvitamin D measured in the pre-conception period before ovarian stimulation in patients undergoing in vitro fertilization with fresh autologous embryo transfer. A total of 306 women were included and adverse pregnancy outcomes in their resulting pregnancy were recorded. Patients who were vitamin D deficient (< 20ng/ml) were compared with those who were non-deficient (≥20ng/ml) and analysed for any association with adverse pregnancy outcomes.</p><p><strong>Results: </strong>A total of 16/306 (5.3%) patients had hypertensive disorders of pregnancy (gestational hypertension and/or pre-eclampsia). The adjusted odds ratio for hypertensive disorders of pregnancy using vitamin D deficiency as a reference was 0.190 (95% CI 0.042-0.852) (p = 0.030). Other pregnancy complications were not significantly different with regards to pre-conception vitamin D status.</p><p><strong>Conclusions: </strong>Pre-conception vitamin D deficiency is associated with an increased risk of hypertensive disorders in pregnancy in women undergoing in vitro fertilization with fresh embryo transfer.</p><p><strong>Trial registration: </strong>HKUCTR 2361 (9th March 2018).</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"4"},"PeriodicalIF":4.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11716185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HnRNPM modulates alternative splicing in germ cells by recruiting PTBP1.","authors":"Peng Lv, Wenchao Xu, Sheng Xin, Yuanxuan Deng, Bin Yang, Dengjianyi Xu, Jian Bai, Deilin Ma, Tao Wang, Jihong Liu, Xiaming Liu","doi":"10.1186/s12958-024-01340-5","DOIUrl":"10.1186/s12958-024-01340-5","url":null,"abstract":"<p><strong>Background: </strong>Heterogeneous nuclear ribonucleoprotein M (HnRNPM) is a key splicing factor involved in various biological processes, including the epithelial‒mesenchymal transition and cancer development. Alternative splicing is widely involved in the process of spermatogenesis. However, the function of hnRNPM as a splicing factor during spermatogenesis remains unknown.</p><p><strong>Methods: </strong>The expression of hnRNPM in germ cells at different stages was detected by polymerase chain reaction, western blotting, a single-cell database, and chromosome spreading assays. Conditional hnRNPM knockout mice were generated to observe the development of testes and germ cells in male mice. Histological staining, immunofluorescence staining and transmission electron microscopy were used to observe the abnormal development of sperm from conditional hnRNPM-deficient mice. Coimmunoprecipitation and mass spectrometry analyses revealed the proteins that interact with hnRNPM. RNA sequencing was performed to analyse the different alternative splicing events in the testes of control and hnRNPM-deficient mice.</p><p><strong>Results: </strong>In this study, we revealed that hnRNPM is highly expressed in spermatocytes and round spermatids, with the exception of XY bodies and metaphase. Therefore, we generated a germ cell-specific hnRNPM conditional knockout mouse model to investigate the role of hnRNPM in spermatogenesis. A lack of hnRNPM led to male infertility under natural conditions. Male hnRNPM-deficient mice presented lower numbers of sperm, lower motility, significantly more malformed sperm and even tailless sperm. Moreover, we found that hnRNPM interacted with PTBP1 to collectively regulate the process of spermatogenesis. In addition, we found that hnRNPM deficiency caused 1617 different alternative splicing events, and we detected abnormal exon skipping events in Cep152, Cyld, Inpp4b and Cd59b.</p><p><strong>Conclusions: </strong>Together, our results suggest that hnRNPM regulates the alternative splicing of mRNAs during spermatogenesis by recruiting PTBP1 and is required for male mouse fertility.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"3"},"PeriodicalIF":4.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medical therapy to treat obesity and optimize fertility in women of reproductive age: a narrative review.","authors":"Janelle Duah, David B Seifer","doi":"10.1186/s12958-024-01339-y","DOIUrl":"https://doi.org/10.1186/s12958-024-01339-y","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Overweight and obesity-chronic illnesses in which an increase in body fat promotes adipose tissue dysfunction and abnormal fat mass resulting in adverse metabolic, biomechanical, and psychosocial health consequences-negatively impact female fertility. Adverse conception outcomes are multifactorial, ranging from poor oocyte quality and implantation issues to miscarriages and fetal health issues. However, with the advent of novel pharmacologic agents, significant weight loss can be achieved, improving the chances of healthy pregnancies, and their use should be considered during periconceptual counseling. There are currently 6 FDA-approved medications for weight loss: 2 GLP1-receptor agonists (GLP1-RAs) liraglutide and semaglutide, 1 dual GLP-1 and gastric inhibitory peptide agonist (GLP1-GIP) tirzepatide, Contrave (naltrexone/bupropion), Qsymia (phentermine/Topamax), and Xenical (orlistat). GLP1-RAs reduce food cravings, appetite, and \"food noise\" and improve insulin sensitivity and satiety, all of which lead to significant weight loss, ranging from 6 to 30% of starting total body weight or greater, depending on the specific agent used. Their efficacy and relative safety should make them first-line options for women seeking to lose weight in the year before trying to conceive. Contrave, the combination of naltrexone and bupropion, seems to work most significantly for weight loss by inhibiting the rewarding and reinforcing effects of food consumption. Clinical trials report ~ 6% loss of starting total body weight with use of Contrave, as well as improvement in metabolic health factors. It may also improve a woman's ability to conceive by mitigating the effects of PCOS and endometriosis and reducing the drive for alcohol and smoking. Qsymia, the combination of phentermine and topiramate, results in more weight loss than Contrave but cannot be used in the acute preconception period, as its topiramate component is a known teratogen. Orlistat is another FDA-approved medication for weight loss; however, it is currently used much less often than other anti-obesity drugs because of its relatively lower efficacy and significant side effects. Bariatric surgery, which can lead to significant weight loss (25-50%), was previously regarded as the most durable method for weight loss, before the advent of GLP1-RAs. Given the inherent risks of surgery, the development of vitamin (i.e. B12, folate, vitamin D) and mineral (i.e. iron, copper, zinc) deficiencies, that may impact the health of the mother and fetus, as well as the recommended delay of 1-2 years prior to attempting pregnancy, bariatric surgery should not be considered first-line therapy for obesity management in women of reproductive age, especially for women who are hoping to conceive quickly or are nearing advanced maternal age.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Clinically significant and meaningful weight loss is achievable with pharmacotherapy to help enhance fertility","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"2"},"PeriodicalIF":4.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities.","authors":"Alberto Sola-Leyva, Bárbara Romero, Analuce Canha-Gouveia, Inmaculada Pérez-Prieto, Nerea M Molina, Eva Vargas, Juan Mozas-Moreno, Clara Chamorro, Merli Saare, Andres Salumets, Signe Altmäe","doi":"10.1186/s12958-024-01330-7","DOIUrl":"10.1186/s12958-024-01330-7","url":null,"abstract":"<p><strong>Background: </strong>A didelphic uterus represents a unique and infrequent congenital condition in which a woman possesses two distinct uteri, each with its own cervix. This anomaly arises due to partial or incomplete merging of the Müllerian ducts during the developmental stages in the womb. Accounting for uterine malformations, a didelphic uterus is a relatively rare condition, affecting approximately 0.5-2% of the population and is considered one of the more uncommon types of uterine abnormalities.</p><p><strong>Methods: </strong>This case report aims to study the physical separation in uterine didelphys and its impact on endometrial microbiome and inflammation, and the patterns of endometrial receptivity observed.</p><p><strong>Results: </strong>Endometrial receptivity analyses revealed a similar receptive state in both uteri, both in the early receptive phase. Differential markers of chronic endometritis, including CD138, and MUM1-positive cells, were observed when comparing endometrial biopsies from both uteri. The right uterus exhibited a higher prevalence of these positive cells. Regarding the microbiome, significant differences were found between the uteri, notably in the right uterus, a clear non-dominance of lactobacilli and the presence of genera such as Staphylococcus, Streptococcus, and Acinetobacter. Additionally, the right uterus presented a less 'favourable' microenvironment, a characteristic that was also reflected in the right cervix; both sites presenting less lactobacilli than the left side samples. A distinct metabolomic signature associated with the physical separation of the uteri contributed to the differences in endometrial milieu.</p><p><strong>Conclusions: </strong>Our study revealed that physical separation, among other factors in uterus didelphys, affects the endometrial microbiome, metabolome, and inflammatory state, with significant microbiome variation observed between the uteri, although similar endometrial receptivity patterns were noted.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"1"},"PeriodicalIF":4.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and energy metabolism abnormalities in polycystic ovary syndrome: from mechanisms to therapeutic strategies.","authors":"Heqiu Yan, Li Wang, Guohui Zhang, Ningjing Li, Yuhong Zhao, Jun Liu, Min Jiang, Xinrong Du, Qin Zeng, Dongsheng Xiong, Libing He, Zhuoting Zhou, Mengjun Luo, Weixin Liu","doi":"10.1186/s12958-024-01337-0","DOIUrl":"10.1186/s12958-024-01337-0","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS), as a common endocrine and metabolic disorder, is often regarded as a primary cause of anovulatory infertility in women. The pathogenesis of PCOS is complex and influenced by multiple factors. Emerging evidence highlights that energy metabolism dysfunction and oxidative stress in granulosa cells (GCs) are pivotal contributors to aberrant follicular development and impaired fertility in PCOS patients. Mitochondrial dysfunction, increased oxidative stress, and disrupted glucose metabolism are frequently observed in individuals with PCOS, collectively leading to compromised oocyte quality. This review delves into the mechanisms linking oxidative stress and energy metabolism abnormalities in PCOS, analyzing their adverse effects on reproductive function. Furthermore, potential therapeutic strategies to mitigate oxidative stress and metabolic disturbances are proposed, providing a theoretical basis for advancing clinical management of PCOS.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"159"},"PeriodicalIF":4.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11670460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Downregulated DUXAP8 lncRNA impedes trophoblast cell proliferation and migration by epigenetically upregulating TFPI2 expression.","authors":"Xiaotong Tang, Yueying Cao, Dan Wu, Lizhou Sun, Yetao Xu","doi":"10.1186/s12958-024-01335-2","DOIUrl":"10.1186/s12958-024-01335-2","url":null,"abstract":"","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"158"},"PeriodicalIF":4.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Müllerian anomalies and endometriosis: associations and phenotypic variations.","authors":"Surya Bhamidipaty-Pelosi, Isaac Kyei-Barffour, Marianna Volpert, Nora O'Neill, Alyssa Grimshaw, Lars Eriksson, Alla Vash-Margita, Emanuele Pelosi","doi":"10.1186/s12958-024-01336-1","DOIUrl":"10.1186/s12958-024-01336-1","url":null,"abstract":"<p><p>Müllerian anomalies are congenital conditions characterized by the incomplete development of the female reproductive tract. Women affected by Müllerian anomalies often display additional malformations of the renal, skeletal, and cardiovascular system, and are at a higher risk for infertility and adverse pregnancy outcomes. Several Müllerian anomalies have been reported in association with endometriosis, but it is unclear if all classes or anatomical variations are associated with the disease. Most importantly, both Müllerian anomalies and endometriosis can manifest with a wide degree of variability, adding further complexity to their poorly defined relationship. Retrograde menstruation occurring in obstructive Müllerian anomalies is a well-accepted mechanism for the development of endometriosis. However, endometriosis can occur following surgical correction of the anomaly or in the absence of obstruction. This suggests that other mechanisms may be involved, although the specific pathogenesis remains elusive. This review provides a comprehensive summary of the current state of clinical research on endometriosis in Müllerian anomalies. This review also highlights research and knowledge gaps, informing the development of future experimental designs to address current limitations including heterogeneity of phenotypes, variable comorbidities, and lack of genetic information.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"157"},"PeriodicalIF":4.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrast induced nephropathy in women with infertility undergoing hysterosalpingography.","authors":"Akin Usta, Ceyda Sancakli Usta, Duygu Lafci, Tuncay Kiris, Eyup Avci","doi":"10.1186/s12958-024-01334-3","DOIUrl":"10.1186/s12958-024-01334-3","url":null,"abstract":"<p><strong>Background: </strong>Contrast-induced nephropathy (CIN) defined as an acute kidney injury following the administration of iodinated contrast medium (CM). Hysterosalpingography (HSG) is a radiologic procedure used to investigate the shape and structure of the uterine cavity and the patency of the fallopian tubes in the evaluation of infertility. To date, there have been no reports evaluating the development of CIN after HSG procedure. Therefore, we investigated whether CIN development occurs in infertile women who underwent HSG and its relationship with clinical and laboratory changes in women who underwent HSG.</p><p><strong>Methods: </strong>This study was undertaken in 65 women who had infertility evaluation, uterine anomalies and/or tubal blockages. CIN was defined as a 25% relative increase, or a 0.5 mg/dL (44 µmol/L) absolute increase, in serum baseline creatinine (SCr) within 72 h of contrast exposure in the absence of alternative conditions. Hysterosalpingography (HSG) was performed using 5-20 ml of contrast medium. All patients performed routine laboratory tests including assessment of serum creatinine and urea and estimated glomerular filtration rates before and 2-3 day after HSG. Statistical analysis was performed with MedCalc Statistical Software Program v22.023 (Ostend, Belgium) program.</p><p><strong>Results: </strong>The mean ages of participants were 29.5 years and mean BMI were 26.2 kg/m2. The rate of CIN was 12.3% and the severe nephropathy was 1.5% in our study population. The baseline SCr level was 0.59 ± 0.06 mg/dL in women with CIN and 0.67 ± 0.11 mg/dL in women without CIN. The baseline SCr level was significantly lower in CIN group that non-CIN group (p = 0.0309). The SCr level significantly higher in CIN group than non-CIN group 48-72 h after HSG (p = 0.0005). In the multivariate logistic regression analysis, the baseline SCr was found an independent risk factor for the prediction of CIN in women who underwent HSG.</p><p><strong>Conclusion: </strong>The HSG procedure is generally a safe method, but the iodine-containing contrast material used in HSG may be associated with temporary adverse effects on kidney function.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"156"},"PeriodicalIF":4.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is oral dydrogesterone equivalent to vaginal micronized progesterone for luteal phase support in women receiving oocyte donation?","authors":"Margaux Lorillon, Geoffroy Robin, Laura Keller, Emeline Cailliau, Clémence Delcourt, Virginie Simon, Christine Decanter, Sophie Catteau-Jonard","doi":"10.1186/s12958-024-01322-7","DOIUrl":"10.1186/s12958-024-01322-7","url":null,"abstract":"<p><strong>Research question: </strong>To determine whether the use of oral dydrogesterone (DYD) in luteal phase support (LPS) during an artificial cycle provides equivalent clinical and ongoing pregnancy, delivery and miscarriage rates as micronized vaginal progesterone (MVP) in oocyte donation recipients.</p><p><strong>Design: </strong>This was a retrospective observational study of prospectively collected data from the assisted reproductive technology (ART) Department of Lille University Hospital from July 2018 to July 2022. All recipients underwent endometrial preparation by an artificial cycle. Luteal phase support (LPS) was provided by weekly intramuscular progesterone (IM) (500 mg/2 ml) and either DYD (40 mg/day) or MVP (800 mg/day) for 12 weeks if the pregnancy test was positive. The primary endpoint was the clinical pregnancy rate.</p><p><strong>Results: </strong>Our study analysed 372 oocyte donation cycles with embryo transfer: 162 embryo transfers with DYD + IM progesterone and 210 embryo transfers with MVP + IM progesterone. After adjustment for confounding factors, our two groups were comparable in terms of clinical pregnancy rates, with 36.7% in the MVP group versus 30.3% in the DYD group (p = 0.55); ongoing pregnancy rates (29,1% versus 25.3%, p = 0.95); miscarriage rates (7.6% versus 4.9%, p = 0.35); and live birth rates (26.7% versus 25.3%, p = 0.86).</p><p><strong>Conclusion: </strong>Oral dydrogesterone seems to be a good alternative to vaginal micronized progesterone for LPS treatment during an artificial cycle, especially in combination with a weekly injection of intramuscular progesterone in the course of oocyte donation.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"154"},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-pregnancy LDL/HDL and total Cholesterol/HDL ratios are strong predictors of gestational diabetes mellitus in women undergoing assisted reproductive technologies.","authors":"Yvonne Liu, Johann-Georg Hocher, Shujuan Ma, Liang Hu, Huijun Chen, Xiaoli Zhang, Fei Gong, Bernhard K Krämer, Ge Lin, Berthold Hocher","doi":"10.1186/s12958-024-01320-9","DOIUrl":"10.1186/s12958-024-01320-9","url":null,"abstract":"<p><strong>Background & objective: </strong>To analyze whether there is an association between pre-pregnancy lipid parameters and gestational diabetes mellitus (GDM) in women undergoing assisted reproductive technologies (ART), a group especially at risk for GDM, and if so, which parameter is associated the strongest.</p><p><strong>Methods: </strong>Data was collected at the Reproductive and Genetic Hospital CITIC-Xiangya in Changsha, China from January 2017 to December 2018. The measured lipid parameters include LDL (low-density lipoprotein), HDL (high-density lipoprotein), TC (total cholesterol), and TG (triglycerides).</p><p><strong>Results: </strong>119 (15.5%) of the 767 patients developed GDM. On average, women who developed GDM were older, had a higher BMI, LDL, TC, and TG, and lower HDL. After adjusting for confounders, LDL and HDL showed a significant association with GDM (p < 0.05), but TC and TG did not. Binary LDL/HDL and TC/HDL ratios showed the strongest association with GDM incidence (OR 1.957 [95%CI 1.258-3.044] and 1.942 [1.243-3.034] respectively). Subgroup analysis showed that an elevated LDL/HDL ratio also increased GDM risk in subgroups with a typically lower prevalence of GDM, such as young women with a low BMI and low blood pressure. Both lipid ratios (LDL/HDL and TC/HD) show strong interactions with baseline age, fasting plasma glucose, and LH.</p><p><strong>Conclusions: </strong>In this cohort of Chinese women undergoing ART, pre-pregnancy LDL/HDL and TC/HDL were associated with GDM the strongest from the lipid parameters and could be useful to estimate GDM risk even before ART treatments and pregnancy.</p><p><strong>Clinical trial number: </strong>NCT03503006 registered on the 21st of March 2018 (on clinicaltrials.gov). https://clinicaltrials.gov/study/NCT03503006?locStr=Changsha,%20Hunan,%20China&country=China&state=Hunan&city=Changsha&cond=ivf&rank=2 .</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"155"},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}