Reproductive Biology and Endocrinology最新文献

{"title":"BEZ235-Mediated PI3K/mTOR dual inhibition improves ovarian follicle survival in a preclinical model.","authors":"Jules Bindels, Laëtitia Bernet, Marlyne Squatrito, Michelle Nisolle, Carine Munaut","doi":"10.1186/s12958-025-01427-7","DOIUrl":"10.1186/s12958-025-01427-7","url":null,"abstract":"<p><strong>Background: </strong>Follicular loss after ovarian tissue cryopreservation and autotransplantation (OTCTP) remains a major challenge due to follicle activation and ischemia. We evaluated BEZ235, a dual PI3K/mTOR inhibitor, as a strategy to improve follicle survival in a preclinical model. Its effects were evaluated during ovarian culture, cryopreservation, and transplantation, including the potential benefit of post-grafting VEGF/G-CSF injections.</p><p><strong>Methods: </strong>Murine ovaries, organotipically cultured with chemotherapeutic treatment (4-HC, 2 µM), with or without supplementation with BEZ235 (1 µM), rapamycin (1 µM), LY294002 (25 µM), or AMH (200 ng/ml) were used to evaluate follicle activation. For cryopreservation studies, those inhibitors were added to the freezing medium, and pathways activation were assessed via Western blot. In vivo, ovaries cryopreserved with or without BEZ235 or rapamycin were autotransplanted under the kidney capsule of mice. A subset of mice received intraperitoneal VEGF/G-CSF injections for five days post-transplantation. Follicle quantification, proliferation and activation marker assessment, and fibrosis evaluation were performed three weeks post-grafting.</p><p><strong>Results: </strong>In vitro, BEZ235 significantly counteracted chemotherapy-induced activation of both Akt and mTOR pathways, whereas rapamycin and LY29400 inhibited only mTOR or Akt, respectively. Similarly, during cryopreservation, only BEZ235 significantly reduced activation of both pathways. AMH did not enhance BEZ235's protective effects. In vivo, ovaries slow-frozen with BEZ235 retained a higher percentage of primordial follicles and showed reduced follicle proliferation and activation compared to both control and rapamycin three weeks after transplantation. Additionally, post-grafting injection of VEGF/G-CSF did not further enhance follicle preservation or reduce fibrosis.</p><p><strong>Conclusion: </strong>Dual inhibition of PI3K/mTOR with BEZ235 provides superior protection of the primordial follicle pool by maintaining follicle dormancy, in both in vitro and in vivo models. These findings highlight BEZ235's potential to enhance OTCTP outcomes, extend graft longevity and improve fertility preservation strategies in women.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"91"},"PeriodicalIF":4.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical outcome using the Q300™ device in a reproductive laboratory environment: an open-label, non-controlled and non-randomized study.","authors":"Yulia Michailov, Eden Amsalem, Natalia Umanski, Valeria Tamadaev, Shevach Friedler, Bozhena Saar-Ryss","doi":"10.1186/s12958-025-01424-w","DOIUrl":"10.1186/s12958-025-01424-w","url":null,"abstract":"<p><strong>Background: </strong>Infertility affects a significant portion of the global population, with male factor infertility contributing to nearly half of all cases. The success of assisted reproductive technologies (ART), such as in vitro fertilization (IVF), depends on selecting high-quality sperm with optimal motility, morphology, and DNA integrity. The Q300™ device offers a novel approach for improving sperm selection. Utilizing an advanced optical imaging system, the Q300™ isolates the morphologically compliant sperm cells sperm for injection into the oocytes. This study aims to assess the usability and preliminary clinical outcome of the Q300™ device under \"real-life conditions\" in a fertility laboratory setting.</p><p><strong>Methods: </strong>Preliminary clinical data collected from Barzilai Medical Center with or without using the technology were compared to the Key Performance Indicators (KPI) for ART laboratories according to the Vienna Consensus.</p><p><strong>Results: </strong>The data suggest that the Q300™ significantly improved day-3 embryo development, blastulation, and cumulative pregnancy rates.</p><p><strong>Conclusions: </strong>By offering a more refined and efficient selection process, the Q300™ has the potential to redefine clinical practice in infertility treatment, providing patients with a higher likelihood of achieving successful outcomes.</p><p><strong>Trial registration: </strong>NCT06232720. Date of registration: 15 February 2023. Enrollment of first subject: 20 August 2023. For more information, visit: https://clinicaltrials.gov/study/NCT06232720 .</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"90"},"PeriodicalIF":4.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multidisciplinary approach to weight management and reproductive care: a retrospective cohort study on weight loss through personalized and patient-centered care.","authors":"Samantha Schon, Jocelyn Spizman, Daria Stelmak, Mark Chames, Stacey Pilarz, Jamila Abdur-Rahman, Charley Jiang, Amal Othman, Marie Menke","doi":"10.1186/s12958-025-01415-x","DOIUrl":"10.1186/s12958-025-01415-x","url":null,"abstract":"<p><strong>Background: </strong>Obesity is a highly prevalent chronic disease with a significant and complex impact on reproduction. National guidelines recommend weight loss prior to pregnancy for patients with obesity to mitigate complications and increase fertility; however, targeted, personalized interventions are limited. The objectives of this study are to describe the implementation of a multidisciplinary program designed specifically for women with reproductive disorders and obesity and to report differences in baseline characteristics and weight loss outcomes between women willing vs. not willing to defer pregnancy attempts to focus on weight loss.</p><p><strong>Methods: </strong>Retrospective cohort study at a university multidisciplinary program for women with reproductive disorders and obesity. All participants from program initiation (November 2021) through July 2023 were included in the analysis. Primary outcome was percent body weight loss at 3 months. Secondary outcomes included weight loss at 6 months and achievement of > 5% and > 10% weight loss at each time point.</p><p><strong>Results: </strong>A total of 237 participants were included in the analysis. The majority of participants (88.2%) desired pregnancy. 63.2% of participants who desired pregnancy were willing to postpone pregnancy attempts/fertility treatments so that they could focus on weight loss for at least 3 months. Participants willing to defer pregnancy attempts achieved a significantly greater weight loss at 3 months compared to those who continued pregnancy attempts (mean - 4.8% vs. -2.5%, p = 0.004) and were more likely to achieve > 10% body weight loss at 3 months (14.0% vs. 2.20%, p = 0.031). Those who achieved > 5% weight loss by 6 months were more likely to achieve pregnancy within the first 6 months of trying to conceive (34.1% vs. 7.7%, p = 0.004).</p><p><strong>Conclusions: </strong>We describe the development and implementation of a multidisciplinary program for women with reproductive disorders and obesity seeking weight management. An individualized approach to weight management and reproductive care results in clinically significant weight loss especially among women willing to defer pregnancy attempts and focus on weight loss for at least 3 months.</p><p><strong>Trial registration: </strong>Clinical trial number: Not applicable.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"89"},"PeriodicalIF":4.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global and regional trends in the burden of surgically confirmed endometriosis from 1990 to 2021.","authors":"Ruijie Li, Ling Zhang, Yi Liu","doi":"10.1186/s12958-025-01421-z","DOIUrl":"10.1186/s12958-025-01421-z","url":null,"abstract":"<p><strong>Background: </strong>endometriosis as a common gynecologic finding significantly affects the quality of life of many women. An accurate understanding of the epidemiological characteristics of endometriosis is essential for disease control and prevention. We aimed to use the latest data from the Global Burden of Disease (GBD) 2021 to comprehensively analyze the various epidemiological indicators of surgically confirmed endometriosis and their changing trends to better measure the disease burden and help improve health management.</p><p><strong>Methods: </strong>We delineated incidence, prevalence, and years lived with disability (YLDs) of surgically confirmed endometriosis at the global, regional, and national levels. The estimated annual percentage change (EAPC) was calculated to assess temporal trends in the age-standardized rate (ASR). In addition, we used joinpoint regression models to describe local trends in these indicators, assessed the correlation between disease burden and Socio-demographic index (SDI) levels, and used decomposition analysis to quantitatively analyze the driving factors leading to changes in disease burden.</p><p><strong>Results: </strong>Globally, the age-standardized rate of incidence, prevalence, and YLDs of surgically confirmed endometriosis all showed a decreasing trend from 1990 to 2021. The burden of surgically confirmed endometriosis is mainly concentrated in women aged 20-30 years and declines with increasing SDI levels. The results of the decomposition analysis indicated that population growth is the main driving factor for the upward in the number of incidence, prevalence, and YLDs cases of endometriosis worldwide.</p><p><strong>Conclusions: </strong>The overall burden of endometriosis has decreased globally from 1990 to 2021, but there are regional disparities. Managing this condition remains a major challenge, and more refined policies and interventions are needed to effectively address the burden of endometriosis.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"88"},"PeriodicalIF":4.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between heavy metal exposure and pregnancy loss: evidence from NHANES 2011-2016.","authors":"Chiyang Yu, Qingxia You, Xue Bai, Fangxiang Mu","doi":"10.1186/s12958-025-01373-4","DOIUrl":"10.1186/s12958-025-01373-4","url":null,"abstract":"<p><strong>Objective: </strong>Previous research suggests that heavy metal exposure may lead to pregnancy loss, but findings have varied. This study focuses on examining the relationship between heavy metal exposure (manganese, selenium, cadmium, lead, mercury) and pregnancy loss.</p><p><strong>Methods: </strong>Utilizing data from the 2011-2016 National Health and Nutrition Examination Survey (NHANES), this study included women between 20-80 years with complete pregnancy history, heavy metal exposure data, and covariate information. Pregnancy loss was self-reported by participants. Blood levels of manganese, selenium, cadmium, lead, and mercury were measured using mass spectrometry. Logistic regression, smooth curve fitting, and weighted quantile sum (WQS) regression were employed to investigate the association between heavy metal exposure and pregnancy loss. Subgroup analyses were conducted to verify the heterogeneity of the results.</p><p><strong>Results: </strong>A total of 3623 eligible women were included, with 1607 reporting pregnancy loss. Blood mercury levels were positively correlated with a higher risk of pregnancy loss (odds ratio 1.06, 95% confidence interval 1.03-1.09, P < 0.001), which remained significant in the two adjusted models. A nonlinear association between mercury levels and pregnancy loss was identified. The heterogeneity in this association was influenced by race, education level, body mass index, and age at menarche. No significant links were detected between pregnancy loss and cadmium, lead, manganese, and selenium. WQS regression highlighted the critical role of mercury in pregnancy loss.</p><p><strong>Conclusion: </strong>Mercury exposure may contribute to a higher risk of pregnancy loss. Reducing heavy metal pollution and minimizing mercury exposure could potentially help improve pregnancy outcomes.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"87"},"PeriodicalIF":4.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prediction model of low birthweight risk in singleton pregnancies reduced from dichorionic twin pregnancies.","authors":"Minyue Tang, Qingfang Li, Guiquan Wang, Jiayu Xu, Saijun Sun, Yimin Zhu","doi":"10.1186/s12958-025-01419-7","DOIUrl":"10.1186/s12958-025-01419-7","url":null,"abstract":"<p><strong>Background: </strong>The incidence of twin pregnancies has risen in recent decades, which is mainly attributable to assisted reproduction technology. Fetal reduction (FR) can significantly reduce the risk of low birthweight (LBW) in twins; however, the LBW risk is higher in singletons reduced from twins than that in primary singletons. Factors including maternal factors and FR timing may affect the LBW risk after FR, but there are no relevant prediction models reported to date. Our main study objective was to develop a nomogram to predict LBW risk in singleton pregnancies reduced from dichorionic (DC) twins.</p><p><strong>Methods: </strong>We retrospectively reviewed and analysed data from women with DC twin pregnancies who underwent FR at Women's Hospital School of Medicine, Zhejiang University between July 2005 and August 2021. Least absolute shrinkage and selection operator (LASSO) regression was used to identify relevant variables associated with LBW. A nomogram was constructed and receiver operating characteristic curve, calibration curves, and decision clinical analysis were used for model performance assessment and visualization. The model was evaluated using cohorts produced by 500 resampling bootstrap analysis to test its stability.</p><p><strong>Results: </strong>A total of 471 patients were enrolled in the analysis. Finally, seven independent predictive factors for LBW were identified and integrated to construct the nomogram, including maternal height, nulliparous, conception method, reasons for FR, gestational age at FR, gestational diabetes, and pregnancy hypertensive disease. The area under the receiver operating characteristic curve of our prediction model was 0.793, which was validated in internal confirmation (0.762) using bootstrap analysis. The nomogram had well-fitted calibration curves. Decision curve analysis demonstrated that the nomogram was clinically useful.</p><p><strong>Conclusion: </strong>We first developed a reliable predictive nomogram for the risk of LBW in DC twin pregnancies reduced to singleton pregnancies, providing a useful guide for clinicians and patients in making appropriate decisions regarding FR.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"86"},"PeriodicalIF":4.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulative live births and predictive factors of emergency oocyte cryopreservation: a retrospective cohort study.","authors":"Zichuan Han, Tian Tian, Nan Zhang, Jiaxiang Wang, Jia Li, Lixue Chen, Wan Yang, Ping Liu, Rong Li, Jie Qiao, Rui Yang","doi":"10.1186/s12958-025-01423-x","DOIUrl":"10.1186/s12958-025-01423-x","url":null,"abstract":"<p><strong>Background: </strong>Oocyte vitrification, a widely utilized assisted reproductive technology for fertility preservation, can address emergencies arising from the unavailability of sperm from the male partner on the day of oocyte retrieval. However, the infrequent and unpredictable nature of emergency oocyte cryopreservation leads to a scarcity of literature on its reproductive outcomes, complicating the provision of informed patient counseling.</p><p><strong>Methods: </strong>This study, conducted between January 2017 and December 2022, included 137 emergency oocyte cryopreservation cycles involving 136 patients and their respective thawed cycles. Descriptive statistics were used to analyze cycle characteristics and oocyte thaw and transfer outcomes, grouped by indication of oocyte vitrification. Univariate and multivariate analyses were performed to identify predictors associated with reproductive outcomes by indication of oocyte vitrification.</p><p><strong>Results: </strong>A total of 137 emergency oocyte cryopreservation-thaw cycles were analyzed, with a median oocyte survival rate of 84.2%, fertilization rate of 57.7%, and high-quality Day-3 embryo formation rate of 33.3%. Of all cycles, 15.3% resulted in no transferable embryos. The cumulative live birth rate (CLBR) for the entire cohort was 29.2%, with 40 live births achieved through both fresh and frozen embryo transfers. Stratified analysis revealed that cycles due to absolute male factor infertility had higher reproductive efficiency, including more oocytes retrieved, a greater number of high-quality embryos, higher implantation rates, and a CLBR of 39.5%, compared to 11.8% in the relative male factor group. Multivariate analysis identified female age, infertility duration, sperm source, number of mature oocytes retrieved and the presence of male infertility factors as key determinants of live birth outcomes.</p><p><strong>Conclusions: </strong>Emergency oocyte vitrification yielded a cumulative live birth rate of 29.2%, with rates differing by clinical indication: 39.5% in the absolute male factor group and 11.8% in the relative male factor group. Moreover, the factors associated with reduced live birth rates differed depending on the underlying indication for vitrification. These findings support the clinical utility of emergency oocyte vitrification and underscore the significant contributions of both female and male factors to reproductive outcomes of oocyte cryopreservation.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"84"},"PeriodicalIF":4.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of long non-coding ribonucleic acid HOXA11-AS in endometriosis therapy.","authors":"Ramanaiah Mamillapalli, Nimisha Gawde, Madeline Fay, Rula Atwani, Irene Moridi, Hugh S Taylor","doi":"10.1186/s12958-025-01420-0","DOIUrl":"10.1186/s12958-025-01420-0","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the function of HOXA11-antisense long non-coding RNA (HOXA11-AS) in endometriosis treatment response.</p><p><strong>Methods: </strong>Tissue samples (ectopic and eutopic endometrium) were obtained from surgically diagnosed subjects with endometriosis (n = 15) and controls (n = 11) without endometriosis after treatment with a progestin. RNA was extracted from these tissues; cDNA was prepared and lncRNA HOXA11-AS levels were measured by quantitative real-time polymerase chain reaction (RT-qPCR). Immortalized endometrial stromal cells from an endometriosis patient (ENDO cell line) were cultured and transfected by HOXA11-AS plasmid and potential target genes were analyzed by RT-qPCR.</p><p><strong>Results: </strong>Progestin therapy led to lower lncRNA HOXA11-AS expression. HOXA11-AS was most decreased in ectopic endometriotic lesions, lower by 81% compared to eutopic endometrium from women with endometriosis. There was no difference in progestin response between eutopic endometrium in endometriosis and normal endometrium from controls. A HOXA11-AS plasmid was used to increase HOXA11-AS expression in an endometriotic cell line. Increased HOXA11-AS led to a significant increase in the expression of genes ITGB3, AKT1, MMP2, and MMP9, which have a role in cell proliferation and tumorigenesis. HOXA11-AS also upregulated the mRNA levels of tumor suppressor and apoptotic regulatory genes PTEN, BCL2 and Caspase3.</p><p><strong>Conclusions: </strong>HOXA11-AS is a critical regulator of normal endometrial development. HOXA11-AS is elevated in endometriosis contributes to its pathophysiology. This long non-coding RNA was decreased in women undergoing endometriosis treatment with progestins. HOXA11-AS regulated several key drivers of disease and repression during treatment likely has a central role in preventing growth and invasion of endometriosis.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"83"},"PeriodicalIF":4.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertility and family-building experiences and perspectives of males with cystic fibrosis.","authors":"Ishaan Jathal, Olivia M Stransky, Catherine E Wright, Asher Prangley, Vin Tangpricha, Raksha Jain, Jennifer L Taylor-Cousar, Kara S Hughan, Sigrid Ladores-Barrett, Natalie E West, Gregory S Sawicki, Traci M Kazmerski","doi":"10.1186/s12958-025-01417-9","DOIUrl":"10.1186/s12958-025-01417-9","url":null,"abstract":"<p><strong>Background: </strong>Nearly all males with cystic fibrosis (MwCF) are infertile and, thus, require the use of assisted reproductive technology (ART) to have biologic children. This study aims to describe the fertility and family-building knowledge, experiences, and care utilization of this population and to compare these findings to the general United States (US) population.</p><p><strong>Methods: </strong>We conducted an anonymous cross-sectional study of self-reported survey data compared to data from the 2017-2019 US National Survey for Family Growth (NSFG). We recruited MwCF age 15 years and older at seven US cystic fibrosis (CF) centers.</p><p><strong>Results: </strong>A total of 532 MwCF (mean age 35.3 ± 11.6 years) completed the survey. 83% knew that almost all MwCF are infertile and 84% were aware that MwCF can have biological children. 71% correctly identified the most common cause of male CF infertility. One third of MwCF stated they had never been told by anyone they were infertile due to their CF (mean age of discussion 19.3 ± 8.8 years). 31% reported being a parent. Among parents, 66% were a parent to a biological child born of a partner's pregnancy, 20% via step-parenthood, 15% adoption, 4.3% surrogacy, and 0.6% foster parenthood. Compared to 44% of NSFG males, 18% of MwCF age 15-49 years reported being a parent to a biological child born of their partner's pregnancy (p < 0.001). Among all MwCF, 82% with a biological child reported that they required medical assistance. Among those age 15-49 years, 87% of MwCF with a biological child required medical assistance compared to 9.4% of NSFG males (p < 0.001). Nearly three-quarters (73%) of MwCF who were biological parents underwent sperm retrieval via a variety of extraction techniques. 91% of those utilizing ART underwent in vitro fertilization and 9% intrauterine insemination of their partner.</p><p><strong>Conclusions: </strong>MwCF face significant disease-related fertility and family-building implications with suboptimal counseling. Most MwCF who are parents pursue biological parenthood via a variety of ART services, but one-third chose alternative paths to parenthood. Further research is needed to best understand and support the family-building of MwCF.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"85"},"PeriodicalIF":4.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and isolation of human testicular peritubular myoid cells and Leydig cells by a combination of ITGA9 and NGFR.","authors":"Sha Han, Liangyu Zhao, Jiaqiang Luo, Chenkun Shi, Chencheng Yao, Zhiyong Ji, Junwei Xu, Shuai Xu, Ruhui Tian, Erlei Zhi, Yuhua Huang, Xin Liu, Yuchuan Zhou, Zhi Zhou, Zheng Li, Peng Li","doi":"10.1186/s12958-025-01389-w","DOIUrl":"10.1186/s12958-025-01389-w","url":null,"abstract":"","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"82"},"PeriodicalIF":4.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}