Reproductive Biology and Endocrinology最新文献

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Effect of LH level on HCG trigger day on clinical outcomes in patients with diminished ovarian reserve undergoing GnRH-antagonist protocol. 接受 GnRH-拮抗剂方案治疗的卵巢储备功能减退患者在 HCG 触发日的 LH 水平对临床结果的影响。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-22 DOI: 10.1186/s12958-024-01280-0
Qianjie Zhang, Kexin Zhang, Yu Gao, Shaojing He, Yicen Meng, Lei Ming, Tailang Yin, Jing Yang, Shuang Wu, Zhongming Zhou, Wei Li, Saijiao Li
{"title":"Effect of LH level on HCG trigger day on clinical outcomes in patients with diminished ovarian reserve undergoing GnRH-antagonist protocol.","authors":"Qianjie Zhang, Kexin Zhang, Yu Gao, Shaojing He, Yicen Meng, Lei Ming, Tailang Yin, Jing Yang, Shuang Wu, Zhongming Zhou, Wei Li, Saijiao Li","doi":"10.1186/s12958-024-01280-0","DOIUrl":"10.1186/s12958-024-01280-0","url":null,"abstract":"<p><strong>Research question: </strong>Does luteinizing hormone (LH) levels on human chorionic gonadotropin (HCG) trigger day (LH<sub>HCG</sub>) affect the clinical outcomes of patients with diminished ovarian reserve (DOR) undergoing gonadotropin-releasing hormone antagonist (GnRH-ant) protocol?</p><p><strong>Methods: </strong>Retrospective analysis fresh embryo transfer cycles of DOR patients who underwent GnRH-ant protocol from August 2019 to June 2023. The participants were divided into different groups according to LH<sub>HCG</sub> level and age. The clinical data and outcomes were compared between groups.</p><p><strong>Results: </strong>In patients with DOR, the HCG positive rate (59.3% versus 39.8%, P = 0.005), embryo implantation rate (34.5% versus 19.7%, P = 0.002), clinical pregnancy rate (49.2% versus 28.4%, P = 0.003), live birth rate (41.5% versus 22.7%, P = 0.005) in LH<sub>HCG</sub> < 2.58 IU/L group were significantly higher than LH<sub>HCG</sub> ≥ 2.58 IU/L group. There was no significant correlation between LH<sub>HCG</sub> level and clinical pregnancy in POSEIDON group 3. In POSEIDON group 4, the HCG positive rate (52.8% versus 27.0%, P = 0.015), embryo implantation rate (29.2% versus 13.3%, P = 0.023), clinical pregnancy rate (45.3% versus 18.9%, P = 0.010) in LH<sub>HCG</sub> < 3.14 IU/L group were significantly higher than LH<sub>HCG</sub> ≥ 3.14 IU/L group. Logistic regression analysis indicated that LH<sub>HCG</sub> level was an independent influencing factor for clinical pregnancy in POSEIDON group 4 patients (OR = 3.831, 95% CI: 1.379-10.643, P < 0.05).</p><p><strong>Conclusions: </strong>LH<sub>HCG</sub> level is an independent factor affecting pregnancy outcome of fresh embryo transfer in DOR patients undergoing GnRH-ant protocol, especially for advanced-aged women. LH<sub>HCG</sub> had a high predictive value for POSEIDON group 4 patients, and LH<sub>HCG</sub> ≥ 3.14 IU/L predicts poor pregnancy outcomes.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"107"},"PeriodicalIF":4.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Consideration of hormonal changes for orthodontic treatment during pregnancy and lactation - a review. 妊娠期和哺乳期正畸治疗对荷尔蒙变化的考虑--综述。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-20 DOI: 10.1186/s12958-024-01281-z
Yujie Zhao, Shengqi Qian, Zhijun Zheng, Juxiang Peng, Jianguo Liu, Xiaoyan Guan, Chengcheng Liao
{"title":"Consideration of hormonal changes for orthodontic treatment during pregnancy and lactation - a review.","authors":"Yujie Zhao, Shengqi Qian, Zhijun Zheng, Juxiang Peng, Jianguo Liu, Xiaoyan Guan, Chengcheng Liao","doi":"10.1186/s12958-024-01281-z","DOIUrl":"10.1186/s12958-024-01281-z","url":null,"abstract":"<p><p>Hormonal changes in pregnant and lactating women significantly affect bone metabolism and overall stress levels, positioning them as a unique group within the orthodontic population. Fluctuations in estrogen, progesterone, prolactin, and other hormones are closely linked to bone remodeling and the periodontal tissue's response to inflammation caused by dental plaque. Hormones such as thyrotropin, leptin, and melatonin also play crucial roles in pregnancy and bone remodeling, with potential implications for orthodontic tooth movement. Additionally, adverse personal behaviors and changes in dietary habits worsen periodontal conditions and complicate periodontal maintenance during orthodontic treatment. Notably, applying orthodontic force during pregnancy and lactation may trigger stress responses in the endocrine system, altering hormone levels. However, these changes do not appear to adversely affect the mother or fetus. This review comprehensively examines the interaction between hormone levels and orthodontic tooth movement in pregnant and lactating women, offering insights to guide clinical practice.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"106"},"PeriodicalIF":4.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell RNA sequencing reveals the effects of high-fat diet on oocyte and early embryo development in female mice. 单细胞 RNA 测序揭示了高脂饮食对雌性小鼠卵母细胞和早期胚胎发育的影响。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-20 DOI: 10.1186/s12958-024-01279-7
Qi Zhu, Feng Li, Hao Wang, Xia Wang, Yu Xiang, Huimin Ding, Honghui Wu, Cen Xu, Linglin Weng, Jieyu Cai, Tianyue Xu, Na Liang, Xiaoqi Hong, Mingrui Xue, Hongshan Ge
{"title":"Single-cell RNA sequencing reveals the effects of high-fat diet on oocyte and early embryo development in female mice.","authors":"Qi Zhu, Feng Li, Hao Wang, Xia Wang, Yu Xiang, Huimin Ding, Honghui Wu, Cen Xu, Linglin Weng, Jieyu Cai, Tianyue Xu, Na Liang, Xiaoqi Hong, Mingrui Xue, Hongshan Ge","doi":"10.1186/s12958-024-01279-7","DOIUrl":"10.1186/s12958-024-01279-7","url":null,"abstract":"<p><strong>Background: </strong>Obesity is a global health issue with detrimental effects on various human organs, including the reproductive system. Observational human data and several lines of animal experimental data suggest that maternal obesity impairs ovarian function and early embryo development, but the precise pathogenesis remains unclear.</p><p><strong>Methods: </strong>We established a high-fat diet (HFD)-induced obese female mouse model to assess systemic metabolism, ovarian morphology, and oocyte function in mice. For the first time, this study employed single-cell RNA sequencing to explore the altered transcriptomic landscape of preimplantation embryos at different stages in HFD-induced obese mice. Differential gene expression analysis, enrichment analysis and protein-protein interactions network analysis were performed.</p><p><strong>Results: </strong>HFD-induced obese female mice exhibited impaired glucolipid metabolism and insulin resistance. The ovaries of HFD mice had a reduced total follicle number, an increased proportion of atretic follicles, and irregular granulosa cell arrangement. Furthermore, the maturation rate of embryonic development by in vitro fertilization of oocytes was significantly decreased in HFD mice. Additionally, the transcriptional landscapes of preimplantation embryos at different stages in mice induced by different diets were significantly distinguished. The maternal-to-zygotic transition was also affected by the failure to remove maternal RNAs and to turn off zygotic genome expression.</p><p><strong>Conclusions: </strong>HFD-induced obesity impaired ovarian morphology and oocyte function in female mice and further led to alterations in the transcriptional landscape of preimplantation embryos at different stages of HFD mice.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"105"},"PeriodicalIF":4.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of energy metabolism anomalies and serum biomarkers in the progression of premature ovarian failure via extracellular vesicles' proteomic and metabolomic profiles. 通过细胞外囊泡的蛋白质组学和代谢组学图谱确定卵巢早衰进展过程中的能量代谢异常和血清生物标志物
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-19 DOI: 10.1186/s12958-024-01277-9
Zhen Liu, Qilin Zhou, Liangge He, Zhengdong Liao, Yajing Cha, Hongyu Zhao, Wenchao Zheng, Desheng Lu, Sheng Yang
{"title":"Identification of energy metabolism anomalies and serum biomarkers in the progression of premature ovarian failure via extracellular vesicles' proteomic and metabolomic profiles.","authors":"Zhen Liu, Qilin Zhou, Liangge He, Zhengdong Liao, Yajing Cha, Hongyu Zhao, Wenchao Zheng, Desheng Lu, Sheng Yang","doi":"10.1186/s12958-024-01277-9","DOIUrl":"10.1186/s12958-024-01277-9","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) is a clinical condition characterized by the cessation of ovarian function, leading to infertility. The underlying molecular mechanisms remain unclear, and no predictable biomarkers have been identified. This study aimed to investigate the protein and metabolite contents of serum extracellular vesicles to investigate underlying molecular mechanisms and explore potential biomarkers.</p><p><strong>Methods: </strong>This study was conducted on a cohort consisting of 14 POF patients and 16 healthy controls. The extracellular vesicles extracted from the serum of each group were subjected to label-free proteomic and unbiased metabolomic analysis. Differentially expressed proteins and metabolites were annotated. Pathway network clustering was conducted with further correlation analysis. The biomarkers were confirmed by ROC analysis and random forest machine learning.</p><p><strong>Results: </strong>The proteomic and metabolomic profiles of POF patients and healthy controls were compared. Two subgroups of POF patients, Pre-POF and Pro-POF, were identified based on the proteomic profile, while all patients displayed a distinguishable metabolomic profile. Proteomic analysis suggested that inflammation serves as an early factor contributing to the infertility of POF patients. For the metabolomic analysis, despite the dysfunction of metabolism, oxidative stress and hormone imbalance were other key factors appearing in POF patients. Signaling pathway clustering of proteomic and metabolomic profiles revealed the progression of dysfunctional energy metabolism during the development of POF. Moreover, correlation analysis identified that differentially expressed proteins and metabolites were highly associated, with six of them being selected as potential biomarkers. ROC curve analysis, together with random forest machine learning, suggested that AFM combined with 2-oxoarginine was the best diagnostic biomarker for POF.</p><p><strong>Conclusions: </strong>Omics analysis revealed that inflammation, oxidative stress, and hormone imbalance are factors that damage ovarian tissue, but the progressive dysfunction of energy metabolism might be the critical pathogenic pathway contributing to the development of POF. AFM combined with 2-oxoarginine serves as a precise biomarker for clinical POF diagnosis.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"104"},"PeriodicalIF":4.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142005128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular regulation of DNA damage and repair in female infertility: a systematic review. 女性不孕症中 DNA 损伤和修复的分子调控:系统综述。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-14 DOI: 10.1186/s12958-024-01273-z
Xiuhua Xu, Ziwei Wang, Luyi Lv, Ci Liu, Lili Wang, Ya-Nan Sun, Zhiming Zhao, Baojun Shi, Qian Li, Gui-Min Hao
{"title":"Molecular regulation of DNA damage and repair in female infertility: a systematic review.","authors":"Xiuhua Xu, Ziwei Wang, Luyi Lv, Ci Liu, Lili Wang, Ya-Nan Sun, Zhiming Zhao, Baojun Shi, Qian Li, Gui-Min Hao","doi":"10.1186/s12958-024-01273-z","DOIUrl":"10.1186/s12958-024-01273-z","url":null,"abstract":"<p><p>DNA damage is a key factor affecting gametogenesis and embryo development. The integrity and stability of DNA are fundamental to a woman's successful conception, embryonic development, pregnancy and the production of healthy offspring. Aging, reactive oxygen species, radiation therapy, and chemotherapy often induce oocyte DNA damage, diminished ovarian reserve, and infertility in women. With the increase of infertility population, there is an increasing need to study the relationship between infertility related diseases and DNA damage and repair. Researchers have tried various methods to reduce DNA damage in oocytes and enhance their DNA repair capabilities in an attempt to protect oocytes. In this review, we summarize recent advances in the DNA damage response mechanisms in infertility diseases such as PCOS, endometriosis, diminished ovarian reserve and hydrosalpinx, which has important implications for fertility preservation.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"103"},"PeriodicalIF":4.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141983142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predictive value of Vitamin D levels in pregnant women on gestational length and neonatal weight in China: a population-based retrospective study. 中国孕妇维生素 D 水平对胎儿身长和新生儿体重的预测价值:一项基于人群的回顾性研究。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-13 DOI: 10.1186/s12958-024-01276-w
Qi Chen, Yuqing Chu, Ruixue Liu, Yang Lin
{"title":"Predictive value of Vitamin D levels in pregnant women on gestational length and neonatal weight in China: a population-based retrospective study.","authors":"Qi Chen, Yuqing Chu, Ruixue Liu, Yang Lin","doi":"10.1186/s12958-024-01276-w","DOIUrl":"10.1186/s12958-024-01276-w","url":null,"abstract":"<p><strong>Background: </strong>Vitamin D deficiency, a common occurrence among pregnant women, is an emerging public health concern worldwide. According to research, prenatal vitamin D deficiency is associated with various complications. This study assessed the vitamin D status of pregnant women in Yanbian, Jilin Province, as well as the correlation and predictive value of their vitamin D levels in relation to gestational length (weeks) and fetal weight, aiming to provide a basis for clinical diagnosis and treatment.</p><p><strong>Methods: </strong>We conducted a population-based retrospective study involving 510 pregnant women from August 2019 to October 2022. Blood samples were collected at 16-20 weeks of gestation for the detection of serum vitamin D levels. Statistical analyses were performed using SPSS 28.0 and R 4.1.0 software. Multifactorial logistic regression analysis was employed to establish whether each variable was a risk factor for deliveries at ≤ 38 gestational weeks and low fetal weight. These results were used to construct a risk prediction model, and the model's predictive efficacy was evaluated. Results or differences with p < 0.05 were considered statistically significant.</p><p><strong>Results: </strong>Multifactorial logistic regression analysis revealed that vitamin D ≤ 14.7 ng/mL(OR: 1.611; 95% CI: 1.120-2.318; P = 0.010), Bone Mineral Density (BMD) T-value ≤-1(OR: 1.540; 95%CI: 1.067-2.223; P = 0.021), and gestational hypertension(OR: 7.173; 95% CI: 1.482-34.724; P = 0.014) were the independent risk factors for deliveries at ≤ 38 gestational weeks. Additionally, vitamin D ≤ 14.7 ng/mL(OR: 1.610; 95%CI: 1.123-2.307; P = 0.009), BMD T-value ≤ -1(OR: 1.560; 95%CI: 1.085-2.243; P = 0.016), and gestational hypertension(OR: 4.262; 95% CI: 1.058-17.167; P = 0.041) were the independent risk factors for low fetal weight (< 3400 g).</p><p><strong>Conclusion: </strong>This study revealed that low vitamin D levels are an independent risk factor for a short gestational length and low fetal weight. Prenatal low BMD T-value and comorbid hypertensive disorders were also found to increase the risk of a short gestational length and low fetal weight.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"102"},"PeriodicalIF":4.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors affecting biochemical pregnancy loss (BPL) in preimplantation genetic testing for aneuploidy (PGT-A) cycles: machine learning-assisted identification. 影响植入前非整倍体基因检测(PGT-A)周期中生化妊娠损失(BPL)的因素:机器学习辅助识别。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-08 DOI: 10.1186/s12958-024-01271-1
José A Ortiz, B Lledó, R Morales, A Máñez-Grau, A Cascales, A Rodríguez-Arnedo, Juan C Castillo, A Bernabeu, R Bernabeu
{"title":"Factors affecting biochemical pregnancy loss (BPL) in preimplantation genetic testing for aneuploidy (PGT-A) cycles: machine learning-assisted identification.","authors":"José A Ortiz, B Lledó, R Morales, A Máñez-Grau, A Cascales, A Rodríguez-Arnedo, Juan C Castillo, A Bernabeu, R Bernabeu","doi":"10.1186/s12958-024-01271-1","DOIUrl":"10.1186/s12958-024-01271-1","url":null,"abstract":"<p><strong>Purpose: </strong>To determine the factors influencing the likelihood of biochemical pregnancy loss (BPL) after transfer of a euploid embryo from preimplantation genetic testing for aneuploidy (PGT-A) cycles.</p><p><strong>Methods: </strong>The study employed an observational, retrospective cohort design, encompassing 6020 embryos from 2879 PGT-A cycles conducted between February 2013 and September 2021. Trophectoderm biopsies in day 5 (D5) or day 6 (D6) blastocysts were analyzed by next generation sequencing (NGS). Only single embryo transfers (SET) were considered, totaling 1161 transfers. Of these, 49.9% resulted in positive pregnancy tests, with 18.3% experiencing BPL. To establish a predictive model for BPL, both classical statistical methods and five different supervised classification machine learning algorithms were used. A total of forty-seven factors were incorporated as predictor variables in the machine learning models.</p><p><strong>Results: </strong>Throughout the optimization process for each model, various performance metrics were computed. Random Forest model emerged as the best model, boasting the highest area under the ROC curve (AUC) value of 0.913, alongside an accuracy of 0.830, positive predictive value of 0.857, and negative predictive value of 0.807. For the selected model, SHAP (SHapley Additive exPlanations) values were determined for each of the variables to establish which had the best predictive ability. Notably, variables pertaining to embryo biopsy demonstrated the greatest predictive capacity, followed by factors associated with ovarian stimulation (COS), maternal age, and paternal age.</p><p><strong>Conclusions: </strong>The Random Forest model had a higher predictive power for identifying BPL occurrences in PGT-A cycles. Specifically, variables associated with the embryo biopsy procedure (biopsy day, number of biopsied embryos, and number of biopsied cells) and ovarian stimulation (number of oocytes retrieved and duration of stimulation), exhibited the strongest predictive power.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"101"},"PeriodicalIF":4.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quercetin enhances decidualization through AKT-ERK-p53 signaling and supports a role for senescence in endometriosis. 槲皮素通过AKT-ERK-p53信号传导增强蜕膜化,并支持衰老在子宫内膜异位症中的作用。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-08 DOI: 10.1186/s12958-024-01265-z
Julia Delenko, Xiangying Xue, Prodyot K Chatterjee, Nathaniel Hyman, Andrew J Shih, Robert P Adelson, Polona Safaric Tepes, Peter K Gregersen, Christine N Metz
{"title":"Quercetin enhances decidualization through AKT-ERK-p53 signaling and supports a role for senescence in endometriosis.","authors":"Julia Delenko, Xiangying Xue, Prodyot K Chatterjee, Nathaniel Hyman, Andrew J Shih, Robert P Adelson, Polona Safaric Tepes, Peter K Gregersen, Christine N Metz","doi":"10.1186/s12958-024-01265-z","DOIUrl":"10.1186/s12958-024-01265-z","url":null,"abstract":"<p><strong>Background: </strong>Patients with endometriosis suffer with chronic pelvic pain and infertility, and from the lack of pharmacologic therapies that consistently halt disease progression. Differences in the endometrium of patients with endometriosis vs. unaffected controls are well-documented. Specifically, shed endometrial tissues (delivered to the pelvic cavity via retrograde menstruation) reveal that a subset of stromal cells exhibiting pro-inflammatory, pro-fibrotic, and pro-senescence-like phenotypes is enhanced in endometriosis patients compared to controls. Additionally, cultured biopsy-derived endometrial stromal cells from endometriosis patients exhibit impaired decidualization, a defined differentiation process required for human embryo implantation and pregnancy. Quercetin, a senolytic agent, shows therapeutic potential for pulmonary fibrosis, a disorder attributed to senescent pulmonary fibroblasts. In rodent models of endometriosis, quercetin shows promise, and quercetin improves decidualization in vitro. However, the exact mechanisms are not completely understood. Therefore, we investigated the effects of quercetin on menstrual effluent-derived endometrial stromal cells from endometriosis patients and unaffected controls to define the signaling pathways underlying quercetin's effects on endometrial stromal cells.</p><p><strong>Methods: </strong>Menstrual effluent-derived endometrial stromal cells were collected and cultured from unaffected controls and endometriosis patients and then, low passage cells were treated with quercetin (25 µM) under basal or standard decidualization conditions. Decidualization responses were analyzed by measuring the production of IGFBP1 and PRL. Also, the effects of quercetin on intracellular cAMP levels and cellular oxidative stress responses were measured. Phosphokinase arrays, western blotting, and flow cytometry methods were performed to define the effects of quercetin on various signaling pathways and the potential mechanistic roles of quercetin.</p><p><strong>Results: </strong>Quercetin significantly promotes decidualization of control- and endometriosis-endometrial stromal cells. Quercetin substantially reduces the phosphorylation of multiple signaling molecules in the AKT and ERK1/2 pathways, while enhancing the phosphorylation of p53 and total p53 levels. Furthermore, p53 inhibition blocks decidualization while p53 activation promotes decidualization. Finally, we provide evidence that quercetin increases apoptosis of endometrial stromal cells with a senescent-like phenotype.</p><p><strong>Conclusions: </strong>These data provide insight into the mechanisms of action of quercetin on endometrial stromal cells and warrant future clinical trials to test quercetin and other senolytics for treating endometriosis.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"100"},"PeriodicalIF":4.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: DNA methylation landscape in pregnancyinduced hypertension: progress and challenges. 更正:妊娠诱发高血压的 DNA 甲基化状况:进展与挑战。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-07 DOI: 10.1186/s12958-024-01270-2
Fengying Deng, Jiahui Lei, Junlan Qiu, Chenxuan Zhao, Xietong Wang, Min Li, Miao Sun, Meihua Zhang, Qinqin Gao
{"title":"Correction: DNA methylation landscape in pregnancyinduced hypertension: progress and challenges.","authors":"Fengying Deng, Jiahui Lei, Junlan Qiu, Chenxuan Zhao, Xietong Wang, Min Li, Miao Sun, Meihua Zhang, Qinqin Gao","doi":"10.1186/s12958-024-01270-2","DOIUrl":"10.1186/s12958-024-01270-2","url":null,"abstract":"","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"22 1","pages":"99"},"PeriodicalIF":4.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liraglutide improves follicle development in polycystic ovary syndrome by inhibiting CXCL10 secretion. 利拉鲁肽通过抑制CXCL10分泌改善多囊卵巢综合征患者的卵泡发育。
IF 4.2 2区 医学
Reproductive Biology and Endocrinology Pub Date : 2024-08-06 DOI: 10.1186/s12958-024-01269-9
Min Zhao, Baoying Liao, Chuyu Yun, Xinyu Qi, Yanli Pang
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