Reproductive Biology and Endocrinology最新文献

{"title":"Granulosa cell RNA-Seq insights into senescence and sphingolipid metabolism disorder in PCOS: aspirin as a potential therapeutic drug.","authors":"Weiwei Shi, Hao Lin, Wu Di, Cong He, Yang Shen","doi":"10.1186/s12958-025-01396-x","DOIUrl":"https://doi.org/10.1186/s12958-025-01396-x","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) is a pivotal cause of anovulatory infertility and the pathogenesis remains elusive. Cellular senescence and sphingolipid metabolism disorder are closely intertwined, and both have been demonstrated present within the granulosa cells of PCOS, while research on the combined impact of senescence and sphingolipids on PCOS-related anovulation is scarce.</p><p><strong>Methods: </strong>Here, we leveraged four datasets of PCOS and executed differential gene expression analysis, engaged in WGCNA, and harnessed machine learning algorithms-including RF, SVM-RFE, and LASSO-to deeply explore the key genes that interact with senescence and sphingolipid metabolism in granulosa cells of PCOS. These key genes were subjected to further analysis to construct a diagnostic model, forecast immune cell infiltration, and identify potential agents. Additionally, within the testosterone-stimulated granulosa cells, we validated the expression of key genes, confirmed senescence and sphingolipids dysregulation, and evaluated the therapeutic efficacy of the candidate agent.</p><p><strong>Results: </strong>Our research pinpointed a set of genes (LYN, PLCG2, STAT5B, MMP9, and IL6R) that showed promise as biomarkers for PCOS-related anovulation and the diagnostic nomogram was developed. These biomarkers were linked to various immune cell types infiltration. In testosterone-stimulated granulosa cells, we observed increased expression of these biomarkers, accompanied by signs of senescence and changes in sphingolipids. Importantly, the potential agent aspirin displayed the ability to ameliorate these two processes.</p><p><strong>Conclusion: </strong>This study highlighted the important value of genes associated with senescence and sphingolipids dysregulation in PCOS. Aspirin targeting senescence could be a promising therapeutic drug for addressing anovulation associated with PCOS.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"61"},"PeriodicalIF":4.2,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal obesity and the incidence of large-for-gestational-age newborns in isolated hypothyroxinemia pregnancies: a comparative cohort study.","authors":"Bin Zhang, Sijie Xi, Zhaolong Zhan, Yinglu Zhang, Fengying Lu, Xiaosong Yuan","doi":"10.1186/s12958-025-01394-z","DOIUrl":"https://doi.org/10.1186/s12958-025-01394-z","url":null,"abstract":"<p><strong>Background: </strong>The synergistic impact of isolate maternal hypothyroxinaemia (IMH) and other modulators on fetal growth outcomes is unknown. This study was aimed to determine whether third trimester IMH [free thyroxine level (FT4) below the the 5th percentile and thyroid stimulating hormone (TSH) between the 5th and 95th percentiles] and prenatal body mass index (BMI) jointly increase the risk of large for gestational age (LGA) deliveries.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 11,478 Chinese pregnant women with laboratory data (including thyroid hormone levels and routine biochemical tests) and hospitalization records from a specialized hospital.</p><p><strong>Results: </strong>The prevalence of obesity (BMI ≥ 30 kg/m²) and IMH was 20.1% (2312/11478) and 4.5% (519/11478), retrospectively. Women with obesity had a 6.96-fold greater risk of IMH (95% CI: 4.58, 10.58) and a 5.88-fold increased risk of LGA (95% CI: 4.87, 7.11) than those with normal weight (BMI < 25 kg/m²), while women with IMH had a 1.32-fold increased risk of LGA (95% CI: 1.05, 1.65) than euthyroid women. The positive associations of LGA risk with obesity and IMH remained robust in sensitivity analyses conducted among women aged < 35 years, primiparas, and those without pregnancy complications. Compared to euthyroid women with normal weight, women with obesity and IMH had a 7.60-fold higher risk of LGA (95% CI: 5.26, 10.97). Additionally, a significant interaction between BMI categories and IMH on LGA was observed (P < 0.013). Subgroup analyses validated this interaction among women with aged < 35 years, multiparity, and non-pregnancy complications.</p><p><strong>Conclusions: </strong>Obesity and IMH in late pregnancy are both associated with an increased risk of LGA newborns, and their coexistence may further amplifies this risk; prenatal BMI and thyroid hormone levels could serve as potential indicators for identifying individuals at elevated LGA risk.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"60"},"PeriodicalIF":4.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction: luteinizing hormone for life's journey.","authors":"Carlo Alviggi, Sandro C Esteves, Robert Fischer, Peter Humaidan","doi":"10.1186/s12958-025-01357-4","DOIUrl":"10.1186/s12958-025-01357-4","url":null,"abstract":"","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 Suppl 1","pages":"59"},"PeriodicalIF":4.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equivalent outcomes of human oocytes after vitrification or slow freezing with a modified rehydration protocol.","authors":"Krzysztof Papis, Karolina Hardej, Ewa Stachowiak, Krystyna Żyżyńska-Galeńska, Piotr Lewandowski, Katarzyna Kozioł","doi":"10.1186/s12958-025-01383-2","DOIUrl":"https://doi.org/10.1186/s12958-025-01383-2","url":null,"abstract":"<p><strong>Background: </strong>According to data from numerous research studies and reviews, the efficiency of the oocyte slow-freezing method is believed to be compromised. Here, we attempt to challenge this notion by showing our retrospective evaluation of the efficiency of the traditional vs. successfully modified method of slow-frozen oocyte recovery compared with that of vitrified oocytes. Specifically, we compared the efficiency of a modified thawing/rehydration system applied to oocytes that had already been slow-frozen with the effects of oocyte vitrification. Moreover, we verified this comparison using chemical activation of slow-frozen vs. vitrified oocytes and parthenogenetic embryo development.</p><p><strong>Results: </strong>Twenty-two and 73 thawing cycles of slow-frozen oocytes were performed using traditional and modified rehydration methods, respectively. For comparison, 105 warming cycles of vitrified oocytes were analyzed. The survival rate of oocytes subjected to the traditional rehydration method was 65.1%. In contrast, significantly higher ratios of 89.8% and 89.7% of oocytes survived the thawing/warming procedure performed according to the modified rehydration procedure or vitrification, respectively (P ≤ 0.0001). Clinical pregnancy and implantation rates tended to be higher after a transfer of embryos developed in the modified rehydration group vs. traditional rehydration group (33.8% and 25.5% vs. 23.5% and 13.8%, respectively) and were comparable to vitrification effects (30.1% and 26.6%). Transfer of embryos developed after modified post-thawing rehydration method resulted in 23 births with 25 healthy and one preterm baby, not significantly different from 28 births reported after oocyte vitrification. Slow-frozen oocytes that were chemically activated after the superior modified rehydration method gave similar survival (91.9% vs. 99.0%), activation (76.0% vs. 64.6%) and blastocyst rates (15.2% vs. 9.4%) in comparison with vitrified oocytes, respectively.</p><p><strong>Conclusions: </strong>The modified post-thawing rehydration method applied to slow-frozen oocytes offers benefits in terms of higher oocyte survival, fertilization, and development or activation rates, comparable to the respective measures of vitrified oocytes and, in clinical settings, high pregnancy, implantation, and birth rates. It may bring new hope to patients who have slow-frozen oocytes stored in IVF clinics.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"58"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11998440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive potential of combined secretomics and image-based morphometry as a non-invasive method for selecting implanting embryos.","authors":"Andrea Palomar, Roberto Yagüe-Serrano, Juan Vicente Martínez-Sanchis, Ignacio Iniesta, Alicia Quiñonero, Pedro José Fernández-Colom, Ana Monzó, José María Rubio, Inmaculada Molina, Francisco Domínguez","doi":"10.1186/s12958-025-01386-z","DOIUrl":"https://doi.org/10.1186/s12958-025-01386-z","url":null,"abstract":"<p><strong>Background: </strong>Non-invasive selection of human embryos for in vitro fertilization purposes is still a major challenge to pursue. Therefore, this study aims to identify non-invasive morphometric and secretomic parameters that reliably select the embryos with the highest likelihood of implantation prior to embryo transfer (ET).</p><p><strong>Methods: </strong>Prospective single-centre cohort study. Thirty-two day 5 blastocysts derived from 28 couples undergoing intracytoplasmic sperm injection (ICSI) and ET between January 2023 and April 2023. Patients were split according to their implantation outcome, confirmed with serum beta-human chorionic gonadotropin (b-hCG) levels > 5 mIU/mL nine days post-SET. Ninety-two proteins involved in embryonic developmental programming were measured in spent blastocyst media (SBM) using a protein extension assay. Sparse PLS-DA (sPLS-DA) was used for principal component analysis. Forty-seven morphometric parameters related to the trophoblast, inner cell mass and blastocele dimension were evaluated in microphotographs of day 5 embryos with ImageJ software. T-test and Mann-Whitney tests were respectively used to compare morphometric measurements and normalized expression of secreted protein (NPx) levels between embryos that implanted or not. Predictive value of models of implantation based on embryo morphometric parameters and secreted proteins.</p><p><strong>Results: </strong>Chi-squared tests showed no significant differences in transferred blastocyst stage, quality, and state between subgroups. Implanting blastocysts (n = 14) presented significantly different morphometric shape descriptors (i.e., internal circularity, internal roundness, internal axis ratio, internal angle and trophoblast mean width) than non-implanting blastocysts (n = 13). Among the quantifiable proteins (86/92) in SBM from eleven implanting and nine non-implanting blastocysts, NPx and sPLS-DA analysis revealed three differentially expressed proteins. Matrilin-2 (MATN2) and legumain (LGMN) were significantly elevated (p < 0.01 in both cases) while thymosin beta-10 (TMSB10) was significantly decreased (p < 0.05) in implanting embryos. Predictive models based exclusively on morphometric or secreted protein profiles accurately discriminated implantation outcomes (AUC > 0.71). The model integrating the blastocysts' internal circularity, internal roundness, internal axis ratio and the NPx of MATN2 and TMSB10 in SBM had exceptional negative and positive predictive power for implantation outcomes (100% and 90.91%, respectively; AUC = 0.93).</p><p><strong>Conclusions: </strong>Morphometric shape descriptors and NPx levels of MATN2 and TMSB10 in SBM emerge as promising candidate markers for non-invasive embryo selection.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"57"},"PeriodicalIF":4.2,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of a novel immortalized human vaginal fibroblast cell line for advanced applications in reproductive health.","authors":"Leong Chi Cheng, Shuoming You, Tingting Ren, Junjun Qiu, Keqin Hua","doi":"10.1186/s12958-025-01393-0","DOIUrl":"https://doi.org/10.1186/s12958-025-01393-0","url":null,"abstract":"<p><strong>Background: </strong>Reproductive health issues related to the vagina, face significant challenges due to the lack of standardized research models. Vaginal fibroblasts, which constitute approximately 55% of the vaginal wall's cellular composition, are crucial for tissue repair, remodelling, and reproductive health. These fibroblasts have broad applications in regenerative medicine and gynaecological treatments. Despite their importance, current research relies primarily on epithelial cells or primary vaginal fibroblasts, but primary fibroblasts are limited by their short lifespan, donor-to-donor variability, and susceptibility to senescence. Immortalized fibroblast lines offer a solution by extending the lifespan and enabling reproducible studies. However, a well-characterized immortalized human vaginal fibroblast line has not been established, highlighting the need for novel models to better understand and address vaginal-associated conditions.</p><p><strong>Methods: </strong>Primary human vaginal fibroblasts were immortalized via the lentiviral transfection of human telomerase reverse transcriptase. The resulting cell line was characterized through histological, immunofluorescent, RT-qPCR and flow cytometry analyses. Proliferation, senescence, gene expression, hormone responsiveness and genomic stability were assessed via quantitative polymerase chain reaction, transcriptome sequencing, gene set enrichment analysis, short tandem repeat profiling, and karyotype analysis.</p><p><strong>Results: </strong>The immortalized human vaginal fibroblasts (ihVFs) retained typical spindle-shaped fibroblast morphology and fibroblast-specific marker expression. Compared with primary vaginal fibroblasts, ihVF exhibited significantly reduced senescence, maintained sustained growth through extended culture passages, and preserved genetic stability. Transcriptome sequencing revealed high gene expression similarity between immortalized and primary fibroblasts, with no significant alterations in oncogenic pathways. PCR and immunofluorescent analyses revealed that ihVFs are responsive to estrogen and progesterone stimulation. Short tandem repeat analysis confirmed the novelty of the immortalized cell line, with no overlap with existing cell databases.</p><p><strong>Conclusions: </strong>The novel ihVF cell line retains key phenotypic, functional, and genetic characteristics of primary vaginal fibroblasts, providing a stable, reproducible, and physiologically relevant model for reproductive health research. This cell line addresses the limitations of primary fibroblasts and has broad applications in tissue engineering, gynaecological disorder research, and drug screening, advancing our understanding of vaginal fibroblast biology and therapeutic interventions.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"56"},"PeriodicalIF":4.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11984242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laparoscopically confirmed endometriosis and the risk of incident NAFLD: a prospective cohort study.","authors":"Hangkai Huang, Zhening Liu, Jiaqi Ruan, Zejun Fang, Chengfu Xu","doi":"10.1186/s12958-025-01391-2","DOIUrl":"https://doi.org/10.1186/s12958-025-01391-2","url":null,"abstract":"<p><strong>Background: </strong>To investigate whether endometriosis is associated with the risk of incident nonalcoholic fatty liver disease (NAFLD).</p><p><strong>Methods: </strong>Data were retrieved from Nurses' Health Study II with participants followed up from 1995 to 2017. A total of 61,649 participants were included in this prospective cohort study. The exposure of this study was laparoscopically confirmed endometriosis. We performed Cox proportional hazard regression analyses to estimate the hazard ratio (HR) and 95% confidence interval (95% CI) of the association between endometriosis and NAFLD.</p><p><strong>Results: </strong>A total of 4,774 incident NAFLD cases were recorded during a 1,313,067 person-years of follow-up. In the multivariable adjusted model, laparoscopically confirmed endometriosis was positively associated with the risk of NAFLD (HR: 1.17, 95% CI: 1.07 - 1.29). The results of the mediation analyses revealed that the association was partly attributable to hysterectomy/oophorectomy (31.6% mediated, 95% CI: 18.8-47.9%), hypercholesterolemia, hypertension and infertility. Further analysis revealed that the interaction effect of age was significant for the association between endometriosis and NAFLD (P = 0.01).</p><p><strong>Conclusions: </strong>Laparoscopically confirmed endometriosis was positively associated with the risk of incident NAFLD. Awareness of the potential NAFLD risk should be raised for clinicians and patients during the regular follow-up of endometriosis.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"55"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of hysteroscopic septum resection on IVF/ICSI outcomes in women with septate uterus: a propensity-score retrospective matching study.","authors":"Wei Zhang, Shunzhi He, Xiaoyan Zhang, Zhenteng Liu, Min Chu, Xuemei Liu, Hongchu Bao","doi":"10.1186/s12958-025-01378-z","DOIUrl":"10.1186/s12958-025-01378-z","url":null,"abstract":"<p><strong>Background: </strong>Hysteroscopic septum resection has been a widespread technique throughout the world in women with a septate uterus. The effectiveness of hysteroscopic septum resection before IVF was equivocal. This study aimed to investigate whether hysteroscopic septum resection could improve IVF outcomes for women with a septate uterus.</p><p><strong>Methods: </strong>This retrospective case-control study was carried out from January 2017 to December 2022. We included subfertile women with septate uterus (n = 147), including 113 women undergoing hysteroscopic septum resection and 34 women opting for expectant management before IVF/ICSI. In addition, we selected 147 women with normal uteri using propensity score matching (PSM). The clinical outcomes of IVF/ICSI were compared by logistic regression and Cox proportional regression in each group.</p><p><strong>Results: </strong>During follow-up time, clinical pregnancy occurred in 66 women who underwent hysteroscopic septum resection (58.41%), compared to 23 women opting for expectant management (67.65%, OR 1.783, 95% CI 0.725-4.380) and 87 women with normal uteri (59.18%, OR 2.601, 95% CI 1.202-5.631). Miscarriage occurred in 12 women who underwent hysteroscopic septum resection (18.18%) versus 5 women who had expectant management (21.74%, OR 1.098, 95% CI 0.323-3.734) and 8 women with normal uteri (9.20%, OR 0.278, 95% CI 0.078-0.997), while preterm birth occurred in 7 women who underwent hysteroscopic septum resection (10.61%) versus 1 women who had expectant management (4.35%, OR 0.509, 95% CI 0.056-4.666) and 9 women with normal uteri (10.34%, OR 1.472, 95% CI 0.291-7.447). Live birth occurred in 54 women following hysteroscopic septum resection (47.79%) compared to 18 women following expectant management (52.94%, OR 1.368, 95% CI 0.589-3.175) and 78 women with normal uteri (53.06%, OR 1.641, 95% CI 0.941-2.864).</p><p><strong>Conclusions: </strong>Women who underwent hysteroscopic septum resection had lower clinical pregnancy rate and higher risk of miscarriage compared to those with normal uteri, but there was no significant difference in live birth rate between the groups.</p><p><strong>Trial registration: </strong>This is a retrospective case-control study.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"53"},"PeriodicalIF":4.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between organophosphate flame retardants metabolites in follicular fluid and reproductive outcomes among women undergoing IVF/ICSI treatment in Southwest China.","authors":"Xiaohong Li, Jiahui Qiu, Zhiwei Gan, Shangwei Li, Xun Zeng","doi":"10.1186/s12958-025-01390-3","DOIUrl":"10.1186/s12958-025-01390-3","url":null,"abstract":"<p><strong>Background: </strong>Previous studies suggest organophosphate flame retardants (OPFRs) negatively affect fertility, but limited research explores their metabolites in follicular fluid and reproductive outcomes.</p><p><strong>Objectives: </strong>To investigate the associations between concentrations of OPFRs metabolites in follicular fluid and the outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) among women undergoing treatment.</p><p><strong>Methods: </strong>Women who underwent IVF/ICSI treatment at the Reproductive Center of West China Second University Hospital, Sichuan University, China, from 2017 to 2020 were recruited. The levels of seven OPFRs metabolites were quantified in follicular fluid collected on the day of oocyte retrieval. Reproductive outcomes were assessed, including key IVF/ICSI outcomes.</p><p><strong>Results: </strong>This study included 401 women. After adjusting for relevant confounders, elevated concentrations of BBOEP (β = -0.08, 95% CI: -0.12 to 0 0.05), BEHP (β = -0.11, 95% CI: -0.17 to 0.05), DnBP (β = -0.23, 95% CI: -0.37 to 0.08), and DPhP (β = -0.12, 95% CI: -0.18 to 0.06) in follicular fluid were inversely associated with the number of good embryos on day 3. Elevated BEHP concentrations were negatively associated with the total number of oocytes (β = -0.04, 95% CI: -0.07 to 0.01). In comparison with the lowest tertile, the highest tertile of DnBP was associated with a 42% reduction in biochemical pregnancy (p-trend = 0.05). Furthermore, the BKMR models revealed inverse associations between OPFRs metabolites mixtures and the number of good embryos.</p><p><strong>Conclusion: </strong>Findings suggest OPFRs may negatively affect IVF/ICSI outcomes, warranting further study on environmental impacts on fertility.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"54"},"PeriodicalIF":4.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barriers and enablers to a healthy lifestyle in people with infertility: a qualitative descriptive study.","authors":"Sophia Torkel, Lisa Moran, Rui Wang, Anthony Villani, Evangeline Mantzioris, Robert J Norman, Stephanie Cowan","doi":"10.1186/s12958-025-01387-y","DOIUrl":"10.1186/s12958-025-01387-y","url":null,"abstract":"<p><strong>Background: </strong>While there is a recognised role of lifestyle (diet and physical activity) in management of infertility, there is limited research exploring the perspectives of people with infertility in relation to lifestyle management. The aim of this study was to understand the barriers and enablers affecting uptake of lifestyle intervention in people with infertility who were using or seeking fertility treatment.</p><p><strong>Methods: </strong>A qualitative descriptive study was performed. Online interviews were conducted with people with infertility who were using or seeking fertility treatment. Interviews explored barriers and enablers to a healthy lifestyle while attempting conception. Interview questions were informed by the Capability, Opportunity, Motivation and Behaviour (COM-B) model and theoretical domains framework (TDF). Interview transcripts were analysed using template analysis. Themes were mapped to the COM-B and TDF, and suggested interventions were developed using the behaviour change wheel method.</p><p><strong>Results: </strong>Nine women and two men completed the interviews. The median age was 38 years (interquartile range 33 to 42 years). Barriers and enablers related to capability (e.g. managing whole-body health and disease), opportunity (e.g. unmet needs from the healthcare system) and motivation (e.g. interplay between lifestyle and emotional state). Suggested intervention components included delivering inclusive programs which accommodate individual needs and providing engaging information which debunks myths and explains the mechanism by which lifestyle promotes fertility.</p><p><strong>Conclusions: </strong>Our study provides novel and rich insights into the unique needs of people with infertility, and has identified several interacting factors which influence their lifestyle behaviours. Our findings highlight that changes at the organisational and policy level are essential to overcome major barriers to lifestyle management by improving access to trustworthy resources with actionable advice, and by improving service provision to deliver multidisciplinary patient-centred care. Future studies should use these findings to co-design interventions and resources to support lifestyle management of infertility.</p><p><strong>Trial registration: </strong>Not applicable.</p>","PeriodicalId":21011,"journal":{"name":"Reproductive Biology and Endocrinology","volume":"23 1","pages":"52"},"PeriodicalIF":4.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}