Probiotics and Antimicrobial Proteins最新文献

{"title":"Postbiotics from Saccharomyces cerevisiae RC016 Cell Wall (Formerly Classified as a Prebiotic): Exploring In Vitro and In Vivo Benefits.","authors":"Valeria L Poloni, María Eugenia Pérez, Franco Escobar, Julieta Luna, Yanina Pereyra, Andrea Cristofolini, Alejandra Magnoli, Lilia Cavaglieri","doi":"10.1007/s12602-025-10492-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10492-8","url":null,"abstract":"<p><p>The objective of this work was to obtain postbiotics derived from the cell wall of Saccharomyces cerevisiae RC016 by applying different strategies and to characterize them in terms of their antitoxin capacity in vitro and their in vivo impact on intestinal integrity, evaluating the modulation of the microbiota and the histomorphometry of the intestine. Moreover, the impact of dried strategies on chemical groups related to food toxin adsorption was analyzed. Nine mechanical and enzymatic cell disruption treatments were assayed using S. cerevisiae RC016 biomass to obtain the postbiotic under study. Then, postbiotics were characterized using high-resolution optical microscopy and assayed for in vitro studies related to their antitoxin activity (adsorption and degradation of aflatoxin B₁). Postbiotics were dried using freeze-dried and spray-dried methods and subjected to FT-IR spectroscopy. Finally, the postbiotic efficacy was determined on an in vivo study conducted on 16 male and female BALB/c mice, divided into two experimental groups, each experimental group (n = 8) separated by sex in different cages (four females and four males): untreated (control) and yeast wall treated (YW); (a) female control; (b) male control; (c) female control + YW; (d) male control + YW. The intestinal microbiota showed significant differences in the counts of LAB and enterobacteria between male and female animals. The histomorphometric analysis showed a significant increase in villi height and width, as well as crypt depth, compared to the control group in male mice with the addition of the postbiotic solution of S. cerevisiae. These findings open new avenues for further optimizing postbiotics' production processes and evaluating their efficacy across diverse conditions and populations.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis FiA2 as an Oxydifficidin-Producing Strain and its Effects on the Growth Performance, Immunity, Intestinal Microbiota, and Resistance to Aeromonas salmonicida Infection in Carassius carassius.","authors":"Tahir Ali Khan, He Liangliang, Kotb A Attia, Shahida Bashir, Xia Ziyuan, Roua A Alsubki, Jie Rang, Shengbiao Hu, Liqiu Xia","doi":"10.1007/s12602-025-10485-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10485-7","url":null,"abstract":"<p><p>In aquaculture, biological control of bacterial infections is a promising strategy that, aside from preventing infections, also benefits the host in several ways. In this study, a Bacillus strain FiA2 isolated from the gut of Carassius carassius (crucian carp) exhibited broad spectrums of activity against multiple aquatic bacterial pathogens. The compound with antibacterial activity was successfully separated and identified as oxydifficidin, with a m/z of 559.47. The oxydifficidin retained its activity even after being exposed to high temperatures, ultraviolet light, proteolytic enzymes, chemical reagents, and alkaline and acidic pH. Furthermore, crucian carp, when supplemented with FiA2 in diet, reduced the infection rate (post-challenged survival rate of 45%), increased the total weight gain by 15.87%, and upregulated the mRNA levels of IGF-1 and IGF-2 (P < 0.05) of muscular tissues. Similarly, the innate immune-related genes in the liver, the spleen, and the head kidney of the fish in the FiA2-supplemented group were significantly upregulated (P < 0.05). In addition, FiA2 modulated the intestinal microbiota, as observed in the FiA2 group of crucian carp; the predominant phyla were Actinobacteriota and Firmicutes, whereas in the control group, Plesiomonas dominated at the genus level. Overall, we conclude that B. velezensis FiA2, a broad-spectrum oxydifficidin-producing strain, resists infection and positively regulates the growth, immunity, and intestinal microbiota of crucian carp and thus can be implemented as a novel probiotic strain for aquaculture.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotic Supplementation Increases Amino Acid Absorption from Plant-Based Meal: A Placebo-Controlled, Randomized, Double-Blind, Crossover Study.","authors":"Christine M Florez, Javier Zaragoza, Jessica Prather, Mandy Parra, Jaci Davis, Amie Vargas, Audrey Ross, Ralf Jäger, Martin Purpura, Simone Guglielmetti, Grant M Tinsley, Lem Taylor","doi":"10.1007/s12602-025-10480-y","DOIUrl":"https://doi.org/10.1007/s12602-025-10480-y","url":null,"abstract":"<p><p>Supplementation of probiotic strains can enhance the absorption of amino acids from protein in the gut. The purpose of this study was to assess if supplementation of a multi-strain probiotic or a postbiotic, consisting of the same strains, would alter the absorption of individual and total amino acids following ingestion of a plant-based meal. Sixteen male participants consumed either probiotic (PRO) or postbiotic (cells inactivated by γ-irradiation; POST), both consisting of L. paracasei LP-DG® (CNCM I-1572) plus L. paracasei LPC-S01 (DSM 26760), or a placebo (PLA) for 2 weeks in a randomized, double-blind, crossover design study separated by a 4-week washout period. During the testing session, blood samples were taken at baseline, 30-, 60-, 120-, and 180-min post-ingestion of a plant-based vegan burger patty. Plasma amino acid levels were analyzed, and percent changes from baseline were assessed using linear mixed-effects models, with the PLA condition as the reference group. There was statistically significant POST condition-by-time interactions for percent changes in alanine, asparagine, citrulline, cystine, glycine, methionine, proline, and total amino acids (p < 0.05, for all). Additionally, there was a statistically significant condition (PRO) by time interactions for cystine (p = 0.02). Two weeks of POST supplementation resulted in significant improvements in amino acid absorption profiles for various individual amino acids and total amino acids compared to PLA. This is the first study to report improved amino acid absorption from a mixed macronutrient meal following a period of postbiotic supplementation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Combined Use of Protein and Postbiotics Feed Supplements in Honey Beehives in Autumn and Spring.","authors":"Eduardo J García-Vicente, María Benito-Murcia, María Martín, Ana Pérez, Noelia Hermosilla, Juan Manuel Alonso, David Risco","doi":"10.1007/s12602-025-10489-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10489-3","url":null,"abstract":"<p><p>Feed supplementation in beehives is a commonly necessary practice in beekeeping today, to address the many risks the honey bees face, mainly the climate change and times of food scarcity, agricultural pesticides, or pathogens such as Varroa destructor or Nosema ceranae. Protein supplements and postbiotics products have been evaluated in beehives feed, obtaining improvements of the strength and sanitary status, but they have not been tested in combination. In this study, two experiences have been carried out in autumn and spring to check the potential beneficial effect of this combination in the beehives. Two monitoring were carried out, before and after 1 month of the application of products, in order to assess the strength (number of bees, amount of brood, brood survival, and pollen/honey reserves) and sanitary status (V. destructor and N. ceranae infestation levels) of the colonies. Firstly, the results obtained in autumn experience showed a reduction of both pathogens in supplemented group. On the other hand, in spring, the combination of protein and postbiotics enhanced the brood survival and honey yield, and decreased the V. destructor infestation. Thus, the combined use of protein and postbiotics as feed supplements could be an important tool to improve the sanitary status after the cold season, and to increase the strength and production of beehives in spring, unifying the benefits of both supplements.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifidobacterium longum Ameliorates Intestinal Inflammation and Metabolic Biomarkers in Mice Fed a High-Fat Diet with Gliadin by Indoleacrylic Acid.","authors":"Ning Wang, Zhangming Pei, Hongchao Wang, Jianxin Zhao, Wenwei Lu","doi":"10.1007/s12602-025-10486-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10486-6","url":null,"abstract":"<p><p>Gliadin, abundant in flour-based foods and processed foods, has been widely researched for allergies. However, the impact of gliadin on the intestinal barrier of healthy individuals and the intervention effect of Bifidobacterium longum (B. longum) are rarely explored. Three strains (JCM1217, CCFM1216, CCFM1218) of B. longum with strong gliadin hydrolysis were screened from 18 strains. This study explored the effects of B. longum on mice with a 10-week high-fat diet and 6% gliadin (HFD + 6%G), assessing duodenal health, lipid metabolism, metabolomics, and gut microbiota in the duodenum and colon changes. Three B. longum strains were screened for gliadin hydrolysis to produce minimal R5 immunopeptide production. All three B. longum strains improved duodenal morphology, reduced intestinal permeability, reduced inflammation (IL-15), and activated tryptophan metabolism. Additionally, alterations in the microbiota of the duodenum and colon were also observed. Linear discriminant analysis (LDA) showed that the HFD + 6% G group significantly increased the abundance of Ileibacterium, Alistipes, Bacteroides, Candidatus, Saccharimonas, Streptococcus, Sediminibacterium, and Odoribacterium in the duodenum. The abundance of Blautia, Butyricimonas, Ruminococcaceae UCG-010, Parabacterioids, and Eubacterium nodatum in the colon was also increased. The B. longum CCFM1216 and B. longum CCFM1218 reversed the abundance of these strains. Specifically, B. longum CCFM1216 enhanced the duodenal barrier with indoleacrylic acid, beneficial for blood lipids and glucose. These strains may be used as probiotics for gliadin-related diseases.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and In Vitro Analysis of Pediococcus pentosaceus MBBL4 Implicated Its Therapeutic Use Against Mastitis Pathogens and as a Potential Probiotic.","authors":"Md Morshedur Rahman, Naim Siddique, Md Abu Ahsan Gilman, Soharth Hasnat, Md Golam Haider, Md Morshedur Rahman, Anup Kumar Talukder, Abu Nasar Md Aminoor Rahman, Tofazzal Islam, Ziban Chandra Das, M Anwar Hossain, M Nazmul Hoque","doi":"10.1007/s12602-025-10484-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10484-8","url":null,"abstract":"<p><p>Pediococcus pentosaceus has the potential to be used as probiotics and biologics amid rising trends of global antimicrobial resistance (AMR) and non-communicable diseases. This study analyzed the genome of P. pentosaceus MBBL4, isolated from healthy cow milk, to assess its probiotic properties and antimicrobial efficacy. The strain was subjected to whole genome sequencing (WGS), assembly, and annotations, alongside phylogenetic and comparative genomic analyses. Additionally, carbohydrate utilization, metabolic pathways, genomic safety, and probiotic potential of MBBL4 were assessed. Its in vitro antimicrobial efficacy against mastitis pathogens was also evaluated. The WGS analysis uncovered many important probiotic traits in MBBL4. Phylogenetic analysis demonstrated a close genetic link with other 15 P. pentosaceus strains, sharing more than 99% of core genes within the pan-genome matrix. MBBL4 demonstrated extensive range of carbohydrate metabolism activity, supported by the presence of several genes encoded enzymes, including a complete elucidated lactose metabolism pathway along with 28 additional metabolic pathway modules. Notably, its genome contains regions associated with gallic acid metabolism and related genes. MBBL4 also harbored genes encoding immunity proteins like enterocin A and lactococcin, and antimicrobial compounds including penocin A, lysozymes, laccase, colicin V, and viguiepinol. Comparative analysis with other probiotic strains revealed seven novel exopolysaccharide biosynthesis proteins and one biofilm-related protein. Moreover, MBBL4 remained sensitive to 90% of the tested antibiotics and carried only a single lincosamide resistance gene (lnuA). It effectively inhibited the growth of two important bovine mastitis pathogens, Staphylococcus aureus D4C4 and Escherichia coli G1C5. These results, along with its low pathogenicity score, support the safety profile of MBBL4 and highlight its potential as bioactive natural therapeutic for mastitis and a promising probiotic candidate.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Peptides: Classification, Mechanism, and Application in Plant Disease Resistance.","authors":"Yuan-Zhen Wu, Jin Wang, Ying-Hui Hu, Qi-Shuo Sun, Rui Geng, Li-Na Ding","doi":"10.1007/s12602-025-10478-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10478-6","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are a class of alkaline, small molecules found widely in nature. This article surveys the classifications of AMPs, delving into their modes of action and their role in controlling significant plant diseases caused by bacteria, viruses, and fungi. It also explores the prospects and challenges in this field, aiming to provide insights for enhancing crop disease resistance, ensuring food security, deepening the understanding of pathogen mechanisms, and protecting ecological balance.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of agr-like Loci in Lactiplantibacillus plantarum and L. paraplantarum and Their Role in Quorum Sensing and Virulence Inhibition of Staphylococcus aureus.","authors":"Weizhe Wang, Ifigeneia Kyrkou, Martin S Bojer, Dina Kalloubi, Abdul Jabbar Kali, Miguel Alena-Rodriguez, Jørgen J Leisner, Stephanie Fulaz, Hanne Ingmer","doi":"10.1007/s12602-025-10476-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10476-8","url":null,"abstract":"<p><p>The pathogenicity of Staphylococcus aureus is largely regulated by the agr quorum sensing (QS) system encoded by agrBDCA, which coordinates virulence factor production through secretion and sensing of auto-inducing peptides (AIPs). agr-like systems are also present in coagulase-negative staphylococci, and several of these encode AIPs that inhibit S. aureus QS. In lactic acid bacteria, a similar locus was previously identified in Lactiplantibacillus plantarum WCSF1 termed lamBDCA. Here, we characterized the lamBDCA locus in L. plantarum LMG 13556 and L. paraplantarum CIRM-BIA 1870, and explored the effects on S. aureus QS. Notably, we found that co-cultivation with L. paraplantarum significantly inhibits S. aureus QS and hemolysin production, while less so for L. plantarum. The inhibition by L. paraplantarum was lost upon disruption of its lamBDCA locus, suggesting that the L. paraplantarum AIP mediates cross-species interference with S. aureus agr activation. Transcriptomic analysis revealed that lamBDCA in L. paraplantarum controls the expression of genes belonging to various functional categories, including stress response and metabolism. The latter includes genes encoding riboflavin (B2 vitamin) biosynthesis, which enabled the growth of the L. paraplantarum lamB mutant in the presence of roseoflavin, a toxic riboflavin analogue. Collectively, our results show that L. paraplantarum CIRM-BIA 1870 interferes with S. aureus virulence gene expression through QS suppression, and they implicate QS in the probiotic properties of L. paraplantarum.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Genome Analysis of Limosilactobacillus fermentum MCC0552 for Probiotic Functionalities and Comparative Genomic Study with Reference Strains.","authors":"Krishna Komatwar, Aravind Sundararaman, Steji Raphel, Prakash M Halami","doi":"10.1007/s12602-025-10467-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10467-9","url":null,"abstract":"<p><p>Limosilactobacillus fermentum MCC0552, a potential probiotic was isolated from a dairy source. L. fermentum MCC0552 has been proven earlier to enhance immuno-modulatory response by alleviating inflammatory and diabetic effects. The objective of this study is to analyse the whole genome sequence of L. fermentum MCC0552 for probiotic functionalities and to compare it with the reference strains. The general genomic features of L. fermentum strain MCC0552 were analysed and a phylogenetic dendrogram was generated by considering the genome sequences of reference strains (L. fermentum IFO 3956, F-6, MTCC 5898 and ATCC 14931 or B1 28) which elucidates their evolutionary lineage. Functional analysis of MCC0552 reveals that the strain possess vitamin biosynthesis gene (thiamine and riboflavin) and mucin binding protein (mubp) with comparatively larger in size. HPLC analysis suggests that the strain MCC0552 synthesises class B vitamins (B1, B2, B6, B9 and B12) which increases its significance in nutritional and nutraceutical applications. Genomic island (GI) prediction shows 19 GI in strain MCC0552 genome were identified. GI regions possessed CRISPR cluster genes, mainly type IE, cas2 and type III-A, nucleotide sugar biosynthesis gene, TA system genes, heavy metal associated genes, etc. On further analysis, the strain exhibited the presence of 2 intact prophage regions which ensure its supplementary probiotic attribute. Thus, the present study elaborates on the probiotic potential of strain MCC0552 at the genome level, revealing its remarkable genomic potential and presenting novel prospects for utilising its unique genetic features in diverse scientific fields.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota and Postbiotic Metabolites: Biotic Intervention for Enhancing Vaccine Responses and Personalized Medicine for Disease Prevention.","authors":"Naheed Mojgani, Sumel Ashique, Mehran Moradi, Masoumeh Bagheri, Ashish Garg, Monika Kaushik, Md Sadique Hussain, Sabina Yasmin, Mohammad Yousuf Ansari","doi":"10.1007/s12602-025-10477-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10477-7","url":null,"abstract":"<p><p>The creation of vaccines has revolutionized several aspects of the game in the fight against transmissible diseases, protecting countless individuals around the globe. Several vaccines against potentially fatal diseases such as diphtheria, pertussis, polio, measles, tetanus, influenza, and smallpox have significantly reduced disease risks and successfully immunized individuals against these serious health threats. The immune response generated by vaccination plays a crucial role in mitigating disease risks by stimulating the production of specific antibodies targeting the relevant pathogens. However, the efficacy of vaccines can vary among communities and people due to several factors, including heredity, age, sex, and preexisting health conditions. The multitude of microbes that call the human digestive tract the microbiota have a pivotal role in regulating immunological reactions to immunization, according to mounting data from both experimental models and research trials. Nutritional supplementation with beneficial microbes, such as probiotic bacteria, has been shown to have immune-related benefits, including enhancements of immune system responses, and has the potential to modify the variety that makes up the microbiota. The effectiveness of vaccines can be enhanced by using probiotics, which work by fostering a balanced gut microbiome. New research reveals that the immune response can be influenced by both live and dormant probiotic bacteria, as well as postbiotics, which are byproducts of the metabolism of probiotics. These substances have immunomodulatory functions and are essential in regulating how the immune system reacts to vaccines. This review aims to summarize the available research, explore the possible immune system functions that could explain these effects, and ultimately speculate about how postbiotics, aimed at the biotic microbiota, could improve the efficacy of vaccines. The review further addresses postbiotics' challenges, regulatory aspects, and future directives for biotherapeutic products that could enhance vaccine efficacy and be tailored for personalized treatments.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}