Ji-Yeon Baek, Ji Hun Kim, Na-Kyoung Lee, Hyun-Dong Paik
{"title":"Heat-killed Lactiplantibacillus plantarum WB3813 and Lactiplantibacillus plantarum WB3814 Alleviate LPS-Induced Inflammatory Damage and Apoptosis in A549 Cells.","authors":"Ji-Yeon Baek, Ji Hun Kim, Na-Kyoung Lee, Hyun-Dong Paik","doi":"10.1007/s12602-025-10496-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10496-4","url":null,"abstract":"<p><p>Chronic obstructive pulmonary disease (COPD) is characterized by chronic lung inflammation and apoptosis. Although paraprobiotics are known for their beneficial effects on respiratory health, research on their effect on COPD is limited. In this study, we investigated the effects of heat-killed Lactiplantibacillus plantarum WB3813 and Lact. plantarum WB3814 on inflammation and apoptosis in lipopolysaccharide (LPS)-induced A549 cells. Both strains demonstrated appropriate probiotic properties and were confirmed to be safe. The results showed that heat-killed lactic acid bacteria (H-LAB) exhibited anti-inflammatory and anti-apoptotic effects. Although LPS exposure reduced cell viability, treatment with the two H-LAB demonstrated a protective effect on the cells. RT-PCR results showed that H-LAB pretreatment reduced COPD-related mRNA expression levels, and western blotting analysis indicated downregulation of the activation NF-κB and intrinsic apoptotic signaling pathways. Additionally, elevated levels of IL-6 and eotaxin induced by LPS decreased. Furthermore, the strains significantly lowered intracellular ROS levels, which were increased by LPS, and promoted radical-scavenging activity. Although the in vivo effects have not yet been elucidated, this study demonstrated that heat-killed or live Lact. plantarum WB3813 and Lact. plantarum WB3814 exhibit anti-inflammatory and anti-apoptotic effects in a lung epithelial cell injury model. These findings provide experimental evidence for the development of functional foods to improve bronchial health and for potential clinical applications in COPD treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A G M Sofi Uddin Mahamud, Ishtiak Ahammed Tanvir, Md Ehsanul Kabir, Ismam Samonty, Md Anamul Hasan Chowdhury, Md Ashikur Rahman
{"title":"Gerobiotics: Exploring the Potential and Limitations of Repurposing Probiotics in Addressing Aging Hallmarks and Chronic Diseases.","authors":"A G M Sofi Uddin Mahamud, Ishtiak Ahammed Tanvir, Md Ehsanul Kabir, Ismam Samonty, Md Anamul Hasan Chowdhury, Md Ashikur Rahman","doi":"10.1007/s12602-025-10501-w","DOIUrl":"https://doi.org/10.1007/s12602-025-10501-w","url":null,"abstract":"<p><p>As unhealthy aging continues to rise globally, there is a pressing need for effective strategies to promote healthy aging, extend health span, and address aging-related complications. Gerobiotics, an emerging concept in geroscience, offers a novel approach to repurposing selective probiotics, postbiotics, and parabiotics to modulate key aging processes and enhance systemic health. This review explores recent advancements in gerobiotics research, focusing on their role in targeting aging hallmarks, regulating longevity-associated pathways, and reducing risks of multiple age-related chronic conditions. Despite their promise, significant challenges remain, including optimizing formulations, ensuring safety and efficacy across diverse populations, and achieving successful clinical translation. Addressing these gaps through rigorous research, well-designed clinical trials, and advanced biotechnologies can establish gerobiotics as a transformative intervention for healthy aging and chronic disease prevention.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuewen Yang, Yanlin Zhang, Ruixiang Sun, Wenjuan Du, Yangyang Liu, Lijun Zheng, Zhihong Ren, Ming Ding Li, Jianguo Xu
{"title":"Preclinical Safety Assessment of the Oral Administration of Lactobacillus plantarum GUANKE in Animal Models.","authors":"Yuewen Yang, Yanlin Zhang, Ruixiang Sun, Wenjuan Du, Yangyang Liu, Lijun Zheng, Zhihong Ren, Ming Ding Li, Jianguo Xu","doi":"10.1007/s12602-025-10498-2","DOIUrl":"https://doi.org/10.1007/s12602-025-10498-2","url":null,"abstract":"<p><p>Probiotics have a long history as fermented food or food supplements. The health benefits and safety profiles of probiotics are strain-specific and should be evaluated individually. The aim of this study was to assess the safety of the Lactobacillus plantarum GUANKE (GUANKE) strain by conducting pharmacological studies, oral toxicity assessments, and investigating the colonization and translocation of GUANKE in experimental animal models. Three pharmacological studies were conducted to examine the effects of oral administration of GUANKE on gastric emptying, bile secretion, and gastric juice secretion. In an acute toxicity study, rats were orally administrated with different doses of GUANKE and monitored for 14 days. In the subacute toxicity study, both rats and beagles were administrated with varying doses of GUANKE for 28 consecutive days to evaluate hematologic, biochemical, and histological effects. The results showed that GUANKE administration did not result in any adverse effect on hematological parameters, biochemical parameters, urinary parameters, and organ indices. Importantly, no translocation of GUANKE to extra-intestinal organs or blood was observed following administration of the CFDA-SE labeled strain. In summary, this study demonstrated the safety of GUANKE intake, which encourages its potential application as a probiotic in clinical trials.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and Functional Analysis of Novel SNPs in Enterocin Genes of Enterococcus faecium GHB21.","authors":"Mohamed Merzoug, Khédidja Mosbahi, Daniel Walker, Nour Eddine Karam, Zohra Yasmine Zater, Svetoslav Dimitrov Todorov, Djamal Saidi","doi":"10.1007/s12602-025-10488-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10488-4","url":null,"abstract":"<p><p>This study investigates the functional and structural impact of single nucleotide polymorphisms (SNPs) in the enterocin and associated immunity genes of Enterococcus faecium GHB21, a strain known for producing potent antimicrobial peptides. Enterocins, most of them classified as class IIa bacteriocins, exhibit strong activity against pathogens such as Listeria monocytogenes, making them promising candidates for food preservation and therapeutic interventions. Using cloning, sequencing, and bioinformatics tools, we analyzed key enterocin genes (entA, entB and entP) and their associated immunity genes (entAi and entPi). Two novel SNPs were identified that result in amino acid substitutions: G15N in pre-enterocin P (EntP), located within the leader sequence, and V36I in the EntPi immunity protein. Additionally, the V9I mutation within the conserved YGNGV motif of the mature EntP peptide and the G48S mutation in the EntAi immunity protein were analyzed. Protein Variation Effect Analyzer classified all mutations as neutral, indicating minimal disruption to protein function. DynaMut analysis revealed that V9I stabilizes EntP but slightly reduces its flexibility, potentially influencing its interaction with target bacteria. Despite these mutations, the enterocins retained critical structural features, including disulfide bonds and β-sheet arrangements, ensuring their antimicrobial efficacy. These findings underscore the structural resilience of enterocins, supporting their application in food safety and in combating multidrug-resistant pathogens.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modulation of the Sporulation Dynamics in the Plant-Probiotic Bacillus velezensis 83 via Carbon and Quorum-Sensing Metabolites.","authors":"Esmeralda Yazmín Soriano-Peña, Agustín Luna-Bulbarela, Sergio Andrés Cristiano-Fajardo, Enrique Galindo, Leobardo Serrano-Carreón","doi":"10.1007/s12602-025-10482-w","DOIUrl":"https://doi.org/10.1007/s12602-025-10482-w","url":null,"abstract":"<p><p>Spore-forming Bacilli, such as the plant-associated Bacillus velezensis strains, are widely used as probiotics, known for their safety and substantial health benefits for both animal and plant species. Through differentiation pathways mediated by quorum-sensing metabolites (QSMs), these bacteria develop multiple isogenic subpopulations with distinct phenotypes and ecological functions, including motile cells, matrix-producing/cannibalistic cells, competent cells, spores, and others. However, the heterogeneity in Bacillus populations is a significant limitation for the development of spore-based probiotics, as nutrients supplied during fermentation are consumed through non-target pathways. One of these pathways is the generation of overflow metabolites (OMs), including acetoin and 2,3-butanediol. This study elucidates, using a 2<sup>3</sup> full factorial experimental design, the individual effects of OMs, QSMs, and their interactions on the sporulation dynamics and subpopulation distribution of B. velezensis 83. The results showed that OMs play a relevant role as external reserves of carbon and energy during in vitro nutrient limitation scenarios, significantly affecting sporulation dynamics. OMs improve sporulation efficiency and reduce cell autolysis, but they also decrease cellular synchronization and extend the period of spore formation. Although QSMs significantly increase sporulation synchronization, the desynchronization caused by OMs cannot be mitigated even with the addition of autoinducer QSM pro-sporulation molecules, including competence and sporulation stimulating factor \"CSF\" and cyclic lipopeptides. Indeed, the interaction effect between OMs and QSMs displays antagonism on sporulation efficiency. Modulating the levels of OMs and QSMs is a potential strategy for regulating the distribution of subpopulations within Bacillus cultures.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoqian Li, Wen Rui, Pingting Shu, Yuhang Sun, Jingpeng Yang
{"title":"Efficacy Evaluation of Selenium-enriched Akkermansia muciniphila in the Treatment of Colon Tumor Mice.","authors":"Xiaoqian Li, Wen Rui, Pingting Shu, Yuhang Sun, Jingpeng Yang","doi":"10.1007/s12602-025-10500-x","DOIUrl":"https://doi.org/10.1007/s12602-025-10500-x","url":null,"abstract":"<p><p>Selenium (Se)-enriched probiotics possess a variety of beneficial properties and are widely used in specialty foods and biomedical applications. Akkermansia muciniphila (AM) is being considered a promising candidate for the \"next generation probiotics (NGPs),\" which play an essential role in the field of tumor therapy. However, there are no studies on the efficacy of Se-enriched A. muciniphila (Se-AM) in the field of tumor therapy. The present study utilized inorganic Se bio-enrichment for the preparation of Se-AM. To evaluate the killing effect of Se-AM on CT26 cells, the actual efficacy and safety of Se-AM were investigated in a mouse model of colon cancer. The results showed that the Se-AM-cell lysate was able to significantly kill CT26 cells, but this killing effect was progressively weakened with decreasing concentrations of Se-AM-cell lysate. The results of animal experiments showed that Se-AM was able to safely and effectively curb the disease course of mice with colon tumors, reduce the tumor volume, lower the levels of IL-1β and IL-6, and increase the levels of TNF-α in the colon of mice. Furthermore, treatment with Se-AM in mice led to a restoration of gut microbiota diversity, reaching levels similar to those observed in healthy controls. This restoration was accompanied by a significant enrichment of beneficial genera, such as Turicibacter, Butyricimonas, Prevotella, and Akkermansia. In summary, Se-AM prepared in this study was able to produce effective killing of CT26 cells directly and played a substantial therapeutic role in a mouse model of colon tumors constructed from CT26 cells. Se-AM had no adverse effect on the heart, liver, spleen, lungs, and kidney of mice and demonstrated a high level of safety. Meanwhile, Se-AM significantly raised the level of the Shannon index and the ratio of Firmicutes to Bacteroidetes of the gut microbiota in mice, indicating its ability to regulate the homeostasis of the microbiota. These results imply that Se-AM has great application value in the field of colon cancer treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jéssika Rodrigues Clementino, Louise Iara Gomes de Oliveira, Mateus Kawata Salgaço, Fellipe Lopes de Oliveira, Victoria Mesa, Josean Fechine Tavares, Ludmilla Silva-Pereira, Breno Vilas Boas Raimundo, Karen Cristina Oliveira, Alexandra Ivo Medeiros, Francyeli Araújo Silva, Katia Sivieri, Marciane Magnani
{"title":"β-Glucan Alone or Combined with Lactobacillus acidophilus Positively Influences the Bacterial Diversity and Metabolites in the Colonic Microbiota of Type II Diabetic Patients.","authors":"Jéssika Rodrigues Clementino, Louise Iara Gomes de Oliveira, Mateus Kawata Salgaço, Fellipe Lopes de Oliveira, Victoria Mesa, Josean Fechine Tavares, Ludmilla Silva-Pereira, Breno Vilas Boas Raimundo, Karen Cristina Oliveira, Alexandra Ivo Medeiros, Francyeli Araújo Silva, Katia Sivieri, Marciane Magnani","doi":"10.1007/s12602-025-10491-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10491-9","url":null,"abstract":"<p><p>β-Glucan is a fermentable polysaccharide with prebiotic properties that has been shown to improve metabolic indicators. This study evaluated the effects of spent brewer's yeast β-glucan (BGL) and Lactobacillus acidophilus LA-5 (10<sup>6</sup> CFU/g) (LA5) alone and in combination (LA5-BGL) on the composition of the fecal microbiome of adults with type 2 diabetes mellitus (T2DM) using the Human Gut Microbial Ecosystem Simulator (SHIME®). Short-chain fatty acids (SCFAs), ammonium ions, and cytokines (IL-6 and IL-10) were measured. BGL, LA5, and LA5-BGL increased (p < 0.05) the richness and diversity of microbial communities in the gut microbiome of individuals with T2DM. All treatments increased (p < 0.05) the abundance of Bacteroides, Alistipes, Lactobacillus, Subdoligranulum, and Acidaminococcus, along with increased (p < 0.05) production of SCFAs and anti-inflammatory cytokine (IL-10) compared to the control group. BGL treatments showed a greater increase in microbial diversity, SCFAs levels (butyric, propionic, and acetic acid), and the anti-inflammatory cytokine (IL-10). LA5 showed the highest decrease in ammonium ion levels. Results indicate that BGL may have a prebiotic and immunomodulatory effect on the fecal microbial community and metabolic indicators in adults with type 2 diabetes mellitus (T2DM). Findings underscore the role of BGL as a prebiotic food.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thayra Gomes Dos Santos, Karina Araujo Gomes Dos Santos, Eddy José Francisco de Oliveira, Bertram Brenig, Elinalva Maciel Paulo, Pedro Henrique Marques, Valbert Nascimento Cardoso, Flávia Figueira Aburjaile, Siomar Castro Soares, Wanderson Marques da Silva, Vasco Azevedo, Rodrigo Dias de Oliveira Carvalho
{"title":"Genomic Scale Analysis Foresees Enteroprotective and Butyrogenic Properties in Brazilian Isolates of Lactiplantibacillus plantarum.","authors":"Thayra Gomes Dos Santos, Karina Araujo Gomes Dos Santos, Eddy José Francisco de Oliveira, Bertram Brenig, Elinalva Maciel Paulo, Pedro Henrique Marques, Valbert Nascimento Cardoso, Flávia Figueira Aburjaile, Siomar Castro Soares, Wanderson Marques da Silva, Vasco Azevedo, Rodrigo Dias de Oliveira Carvalho","doi":"10.1007/s12602-025-10495-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10495-5","url":null,"abstract":"<p><p>Probiogenomics offers a rapid and comprehensive approach to characterizing the beneficial properties of microorganisms, allowing the identification and selection of specific strains for improving human health. The species Lactiplantibacillus plantarum is highly ubiquitous and presents great genetic diversity making it difficult to conduct massive screening of probiotic strains when using conventional methods. Hence, this work aimed to taxonomically and functionally characterize the complete genomes of six novel L. plantarum strains isolated in Brazil through high-standard NGS. The taxonomy of strains E6, E10, E14, E18, Lpl2, and Lpl4 was investigated through average nucleotide identity analysis using the genome of L. plantarum SK151 from the GENBANK database as a reference. Moreover, multilocus phylogenetic analyses were performed to investigate their genetic relatedness to probiotic L. plantarum strains using a total of 225 complete genomes from the same database. To investigate specific health-promoting and safety properties, the isolates from Brazil were analyzed using several bioinformatic tools to predict antimicrobials and anti-inflammatory biomolecules and potential risk factors including drug resistance, virulence genes, and genetic mobility elements. The results confirmed that all six isolates belong to the species L. plantarum and the phylogenetic analysis suggests high proximity to known probiotics and strains that have demonstrated protective effects against inflammation and pathogens. Several bacteriocin genes were identified in the Brazilian isolates, mostly plantaricins, suggesting antimicrobial properties, especially in the strain E14 that presented 9 bacteriocins genes. Furthermore, the study revealed the potential capacity of these strains to produce anti-inflammatory compounds including metabolites such as butyrate through the fermentation of fructan sugars, and IL-10 stimulation activity mediated by adhesin proteins from the bacterial cell wall. The lpl4 strain glutamate decarboxylase (GADB) gene showed a high degree of genetic and structural conservation prediction and increased stabilization of the predicted protein compared to a functional homologous GADB from L. plantarum Taj-Apis362, suggesting lpl4 potential for the modulation of the gut-brain axis functions by the production of GABA. Regarding the presence of virulence and resistance genes, only one resistance island was observed in strain E6 genome. In conclusion, it is possible to suggest that most of the evaluated strains are safe and we highlight the strains Lpl4 and E14 for presenting outstanding enteroprotective properties such as the potential production of GABA and several bacteriocins.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zehranur Yuksekdag, Reyhan Kilickaya, Filiz Kara, Berat Cinar Acar
{"title":"Biogenic-Synthesized Silver Nanoparticles Using the Ligilactobacillus salivarius KC27L Postbiotic: Antimicrobial, Anti-Biofilm, and Antioxidant Activity and Cytotoxic Effects.","authors":"Zehranur Yuksekdag, Reyhan Kilickaya, Filiz Kara, Berat Cinar Acar","doi":"10.1007/s12602-025-10481-x","DOIUrl":"https://doi.org/10.1007/s12602-025-10481-x","url":null,"abstract":"<p><p>This study aimed to synthesize silver nanoparticles (AgNPs) using the postbiotic of the Ligilactobacillus salivarius KC27L strain and evaluate their multifunctional biological properties. The use of L. salivarius, a probiotic bacterium known for its ability to produce a wide range of metabolites, plays a crucial role in this process by acting as a natural, eco-friendly reducing, and stabilizing agent during AgNP synthesis. This approach not only eliminates the need for hazardous chemicals typically used in nanoparticle synthesis but also enhances the biocompatibility and biological efficacy of the resulting nanoparticles. Synthesized AgNPs were analyzed by Fourier transform infrared spectroscopy, FTIR (metabolites of postbiotic); UV-vis (peak of 435 nm); scanning electron microscope, SEM; transmission electron microscopy, TEM (spherical shapes, sizes < 50 nm), energy-dispersive spectrometry, EDS (peak at 3 keV); and zeta potential (- 18.6 mV). These nanoparticles (0.156-40 mg/mL) were evaluated for the antimicrobial and anti-biofilm activities against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 35984, and Streptococcus mutans ATCC 25175, and antioxidant activities using four different methods (2,2-diphenyl-1-picrylhydrazyl free radical scavenging, metal ion chelating, hydroxyl radical scavenging, and superoxide anion scavenging activities). Also, the cytotoxic activity was investigated against a normal cell line (L929) for 24, 48, and 72 h. At a concentration of 40 mg/mL, the AgNPs demonstrated the highest antimicrobial efficacy, with inhibition zones measured as 14.9 mm for P. aeruginosa, 9.5 mm for E. coli, 15.7 mm for S. epidermidis, and 12.9 mm for S. mutans. The AgNPs exhibited anti-biofilm activities against all Gram-positive and Gram-negative bacteria strains studied. According to the DPPH method, the highest antioxidant activity was determined at 40 mg/mL AgNP concentration (80.93%). AgNPs were found to have no toxic effect at low concentrations (0.39-25 µg/mL). Biogenic synthesized AgNPs could be used in biotechnological applications (biomaterials, health, environmental, etc.) with antibacterial, anti-biofilm, antioxidant, and nontoxic properties. However, further research is needed to understand the mechanisms of action of the particles fully.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Heterologous Expression of Bacteriocins from the Metagenome Mining of Cotija Cheese.","authors":"Alfredo Esquivel-López, Diana Rocha-Mendoza, Carlos Eduardo Serrano-Maldonado, Alejandra Escobar-Zepeda, Maricarmen Quirasco","doi":"10.1007/s12602-025-10483-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10483-9","url":null,"abstract":"<p><p>Bacteriocins are a heterologous group of ribosomal peptides with antibacterial activity. They are of interest to the pharmaceutical and food industries due to their potential to fight antibiotic-resistant pathogens and improve microbial food safety, respectively. Metagenomic data mining for antibacterial activity is valuable for the information it provides from unstudied genomic sequences. Furthermore, the higher biosynthetic yield obtained by the heterologous expression of putative bacteriocins allows their subsequent purification and characterization. This work aimed to express antilisterial bacteriocins in Escherichia coli after obtaining their gene sequences by in silico mining the bacterial metagenome of Cotija cheese. This artisanal Mexican cheese is manufactured with unpasteurized milk and ripens for at least 3 months. Analyzing the Cotija cheese bacterial shotgun metagenome allowed us to select two sequences (QC1 and QC2) encoding novel Class IId bacteriocins belonging to the lactococcin family. These genes were expressed as (His)6-fusion proteins in E. coli BL21 (DE3) and showed high antimicrobial activity against Listeria monocytogenes, with a minimum inhibitory concentration of 78 µg/mL. QC1 and QC2 were tested against several pathogenic bacteria and showed activity exclusively against L. monocytogenes. QC2 has a novel sequence that showed no matches against the UniProt database. It was purified by Ni<sup>2+</sup> affinity chromatography and retained its activity after heating at 70 °C for 30 min. As the sequences were obtained by genomic mining on a fermented food metagenome, QC1 and QC2 have potential applications as sanitizers in industrial food facilities where L. monocytogenes contamination is the most prevalent.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}