Probiotics and Antimicrobial Proteins最新文献

{"title":"Synthesis, Characterization, and Biomedical Applications of Bacteriocin-Selenium Nanoconjugates.","authors":"Sana M H Al-Shimmary, Amina N Al-Thwani","doi":"10.1007/s12602-024-10420-2","DOIUrl":"https://doi.org/10.1007/s12602-024-10420-2","url":null,"abstract":"<p><p>The antibiotic overuse in hospitals, the food industry, and animal feed over past times has led to a significant rise in the incidence of antibiotic-resistant bacteria. To address these potentially life-threatening antibiotic-resistant illnesses, a quick identification and development of novel antimicrobials are necessary. The aim of this study was to synthesize a novel bacteriocin-nanoconjugates by combining selenium nanoparticles with purified bacteriocin from the Enterococcus faecium SMAA23 and investigate some of its biomedical activities. The nanoconjugates were characterized via X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray desorption (EDX), and zeta potential analytical techniques. There is investigation of the antibacterial, antifungal, and anticancer properties of nanoconjugates. Purified bacteriocin has a known molecular weight of approximately 43,000 Daltons. The characterization of nanoparticles and nanoconjugates was performed. The crystallite size of nanoconjugate was determined via X-ray diffraction (XRD) to be 15.29 nm. Transmission electron microscopy (TEM) detected particles of irregular form of nanoconjugate, measuring between 11 and 24 nm in diameter. Energy dispersive X-ray spectroscopy (EDX) confirmed the presence of selenium and protein. The measured zeta potential was - 12.1 + 0.12 mV. The results revealed potent antibacterial activity against Acinetobacter baumannii, with a growth inhibition zone of 23 mm ± SD. A minimum inhibitory concentration (MIC) of nanoconjugate was 15.625 µg/mL, while a minimum bactericidal concentration (MBC) was 31.25 µg/mL. The application of scanning electron microscopy (SEM) enhanced the rupture of the bacterial cell wall. The antifungal activity against C. albicans and C. tropicalis resulted in growth inhibition zones of 14 mm and 16 mm (± SD), respectively. Various concentrations of the nanoconjugate strongly inhibited MDA-MB-231 cells in the MTT experiment. The novel synthesized bacteriocin-nanoconjugates exhibited substantial antibacterial, antifungal, and anticancer properties.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic Supplements Benefit Psoriasis Therapy Rather Than Affecting Disease Risk: Evidence from NHANES Machine-Learning and Meta-Analysis Study.","authors":"Yajia Li, Xiao Liu, Ziqin Cao, Jianhuang Wu","doi":"10.1007/s12602-024-10418-w","DOIUrl":"https://doi.org/10.1007/s12602-024-10418-w","url":null,"abstract":"<p><p>This research aimed to evaluate the impact of probiotic supplementation on the severity of psoriasis symptoms and its association with the risk of developing the disease. We analyzed the association between probiotic usage and psoriasis among 21,942 participants from the 2009-2014 NHANES, employing advanced machine learning techniques for data analysis. A separate meta-analysis was conducted to assess the effects of probiotics on the severity, life quality, and some related inflammation factors. Dichotomous data were analyzed using odds ratios (ORs) corresponding to the 95% confidence interval (CI). Analysis of NHANES data did not reveal a statistically significant association between probiotic intake and the risk of psoriasis. However, the meta-analysis indicated that probiotic supplementation significantly lowers Psoriasis Area and Severity Index (PASI) scores both for change in PASI score and post-data change, and enhances PASI 75 (OR = 4.80, 95% CI = 2.92-7.89) and clearance responses (OR = 3.14, 95% CI = 1.99-4.96), as well as reduced levels of inflammatory markers. While the improvement of life quality was only observed in the post-treatment data. While probiotic supplements do not appear to reduce the risk of psoriasis, they have a significant positive effect on reducing disease severity. Registered on PROSPERO: Registration number CRD42023484417.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Impact of Dietary and Water-Based Probiotics on Tilapia Health and Resistance to Aeromonas hydrophila.","authors":"Amany M Diab, Fatma Abd El-Rahman, Malik M Khalfallah, Abdallah S Salah, Foad Farrag, Shawky Ibrahim Darwish, Mustafa Shukry","doi":"10.1007/s12602-024-10415-z","DOIUrl":"https://doi.org/10.1007/s12602-024-10415-z","url":null,"abstract":"<p><p>Aquaculture plays a critical role in global seafood production. Probiotics in aquaculture have gained popularity for enhancing fish health, growth, and water quality. This study aimed to evaluate the effects of probiotic supplementation through different methods-feed and/or water-on Nile tilapia's growth, health, and resistance to Aeromonas hydrophila. A total of 270 Nile tilapia fingerlings (initial weight 10 ± 05 g) were randomly divided into four experimental groups: (1) control group (basal diet with no probiotics), (2) feed additive group (basal diet + probiotics in feed), (3) water additive group (basal diet + probiotics in water), and (4) combined feed and water additive group (basal diet + probiotics in both feed and water). The trial lasted for 12 weeks. The study assessed water quality, growth performance, feed efficiency, digestive enzyme activities, intestinal morphometry, biochemical and immunological parameters, oxidative status, and resistance to A. hydrophila. The results revealed significant improvements in water quality (reduction in TAN and NH3), growth performance, feed utilization, digestive enzyme activity (amylase, lipase), and immune responses in all probiotic-treated groups compared to the control. The group receiving probiotics in both feed and water showed the highest growth, survival rate, and immune function improvements. Additionally, gene expression analysis revealed upregulation of immune-related cytokines (IL-1β, TGF-β, TNF-α) in this group. In conclusion, administering probiotics via both feed and water significantly enhances Nile tilapia's health, growth, and resistance to bacterial infections, with the combined approach yielding the best results. This study highlights the importance of optimizing probiotic administration methods to maximize benefits in aquaculture systems.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic Mixture Attenuates Colorectal Tumorigenesis in Murine AOM/DSS Model by Suppressing STAT3, Inducing Apoptotic p53 and Modulating Gut Microbiota.","authors":"Hoi Kit Matthew Leung, Emily Kwun Kwan Lo, Congjia Chen, Fangfei Zhang, Felicianna, Marsena Jasiel Ismaiah, Hani El-Nezami","doi":"10.1007/s12602-024-10405-1","DOIUrl":"https://doi.org/10.1007/s12602-024-10405-1","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is one of the most common cancers worldwide. The standard CRC chemo drug, 5-Fluorouracil (5-FU), has a poor response rate and chemoresistance, prompting the need for a more effective and affordable treatment. In this study, we aimed to evaluate whether Prohep, a novel probiotic mixture, would alleviate azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colorectal tumorigenesis and enhance 5-FU efficacy and its mechanism. Our results suggested that Prohep showed stronger anti-tumorigenesis effects than 5-FU alone or when combined in the AOM/DSS model. Prohep significantly reduced the total tumor count, total tumor size, caecum weight, colonic crypt depth, colonic inflammation, and collagen fibrosis. Prohep downregulated pro-inflammatory TNF-α and proliferative p-STAT3 and upregulated apoptotic p53. Metagenomics analysis indicated that Prohep-enriched Helicobacter ganmani, Desulfovibrio porci, Helicobacter hepaticus, and Candidatus Borkfalkia ceftriaxoniphila were inversely correlated to the total tumor count. In addition, Prohep-enriched Prevotella sp. PTAC and Desulfovibrio porci were negatively correlated to AOM/DSS enriched bacteria, while forming a co-existing community with other beneficial bacteria. From KEGG analysis, Prohep downregulated CRC-related pathways and enhanced pathways related to metabolites suppressing CRC like menaquinone, tetrapyrrole, aminolevulinic acid, and tetrahydrofolate. From Metacyc analysis, Prohep downregulated CRC-related peptidoglycan, LPS, and uric acid biosynthesis, and conversion. Prohep elevated the biosynthesis of the beneficial L-lysine, lipoic acid, pyrimidine, and palmitate. Prohep also elevated metabolic pathways related to energy utilization of lactic acid-producing bacteria (LAB) and acetate producers. Similarly, fecal acetate concentration was upregulated by Prohep. To sum up, Prohep demonstrated exceptional anti-tumorigenesis effects in the AOM/DSS model, which revealed its potential to develop into a novel CRC therapeutic in the future.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Probiotic Potential: Genomic Insights into Weissella paramesenteroides UFTM 2.6.1.","authors":"Beatriz Macedo de Oliveira Rocha, Yasmin Neves Vieira Sabino, Thaís Costa de Almeida, Fabio Bignoto Palacio, Isabela Sguilla Rotta, Vanessa Cordeiro Dias, Vânia Lúcia da Silva, Cláudio Galuppo Diniz, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Siomar de Castro Soares, Aline Dias Paiva, Julliane Dutra Medeiros, Alessandra Barbosa Ferreira Machado","doi":"10.1007/s12602-024-10409-x","DOIUrl":"https://doi.org/10.1007/s12602-024-10409-x","url":null,"abstract":"<p><p>Weissella, a genus of lactic acid bacteria, has diverse beneficial attributes including probiotic activity and biotechnological applications. Therefore, the investigation of the Weissella genus has garnered growing interest. In this study, we sequenced the complete genome of Weissella paramesenteroides UFTM 2.6.1 isolated from unpasteurized cow's milk from the Triângulo Mineiro region and performed probiogenomic analyses. Taxonomic characterization confirmed the identity of W. paramesenteroides. The genome comprises 1926 protein-coding genes, mainly related to cell metabolism, information storage and processing, and cellular processes and signaling. Ninety-nine unique genes associated with probiotic functions were identified in the genome of W. paramesenteroides UFTM 2.6.1, including genes involved in stress response, bacterial persistence in the gastrointestinal tract, and biosynthesis of vitamins. In silico analysis of bacteriocin-related genes identified Pediocin, and subsequent in vitro testing confirmed that W. paramesenteroides UFTM 2.6.1 exhibits antimicrobial activity against Listeria spp. Genomic characterization revealed the presence of the replicon pLCK4 and four prophage regions, one of which was intact. Moreover, no CRISPR-Cas array or associated Cas proteins were found, along with an absence of resistance and virulence genes, suggesting a safety aspect of the evaluated strain. Pan-genome analysis unveiled 204 exclusive genes in the genome of W. paramesenteroides UFTM 2.6.1, which includes metabolism and stress-associated genes. In general, the results indicate probiotic potential of W. paramesenteroides UFTM 2.6.1. Further studies are required to ensure the safety and beneficial effects of this bacterium in vivo, aiming for future applications in the food industry and animal and human medicine.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Colon Cancer Effects of Lysate from Potential Probiotic Yeast Strains Derived from Human Breast Milk.","authors":"Yi Wu, Chen Li, Zhiyuan Geng, Lihong Wang, Shufeng Wang, Dingxing Zhang, Dongxu Li, Fanyu Meng","doi":"10.1007/s12602-024-10410-4","DOIUrl":"https://doi.org/10.1007/s12602-024-10410-4","url":null,"abstract":"<p><p>Breast milk contains a diverse array of symbiotic microorganisms, including beneficial probiotics. While most research has focused on the bacterial composition of breast milk, the fungal community (mycobiota) has received limited attention. In this study, we isolated and evaluated yeast strains from breast milk, demonstrating the safety and probiotic potential of four strains through hemolysis tests, antimicrobial activity, tolerance to gastrointestinal conditions, aggregation, cell adhesion, and antioxidant activity assays. These yeast strains significantly inhibited the proliferation of pathogenic bacteria and showed strong antioxidant properties. Moreover, lysates from Rhodotorula mucilaginosa and Cryptococcus laurentii isolated from breast milk markedly inhibited the proliferation of colon cancer cell lines (HT-29 and SW620) and induced apoptosis by upregulating key apoptosis-related genes (caspase 3, caspase 9, and Bax), as well as autophagy-related genes (Beclin-1, LC3, and ATG5). This study provides valuable insights into the potential of breast milk-derived yeast strains for developing novel probiotic therapies for colon cancer treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory Effects of Lactiplantibacillus plantarum Strain RW1 During Salmonella Infection in Murine Intestinal Epithelial Cells and Dextran Sulfate Sodium-Induced Murine Colitis.","authors":"Guangzhi Zhang, Abdul Raheem, Xiaofeng Xu, Xuezheng Fan, Jianwei Zhang, Tong Qin, Li Jia, Min Yue, Weifang Yang, Liang Wang, Mingyan Wang, Yajie Yin, Qiuchen Li, Ying Jiang, Ye Tian, Hui Jiang, Ting Xin, Jiabo Ding","doi":"10.1007/s12602-024-10411-3","DOIUrl":"https://doi.org/10.1007/s12602-024-10411-3","url":null,"abstract":"<p><p>Inflammatory diseases resulting from bacterial infections or inflammatory bowel disease pose significant threats to the health of both animals and humans. Although probiotics have emerged as a crucial preventive and adjunctive therapy for these conditions, the precise mechanisms through which probiotics regulate inflammatory diseases remain incompletely understood. In our previous study, animal-derived Lactiplantibacillus plantarum strain RW1 (L. plantarum RW1) with probiotic potential was isolated and characterized. In this study, the signaling pathway of L. plantarum RW1 inhibiting the inflammatory response of mouse intestinal epithelial cells caused by Salmonella infection was studied. Our results revealed that infection of Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC14028 (S. Typhimurium ATCC14028) and Salmonella enterica subsp. enterica serovar Typhimurium strain SL1344 (S. Typhimurium SL1344) significantly increased NF-κB/p65 and TLR4 mRNA levels while decreasing IκB and TLR2 mRNA levels. Whereas L. plantarum RW1 treatment significantly reversed these changes. Western blotting confirmed these findings. Additionally, we explored the protective effects of L. plantarum RW1 in a murine colitis model induced by dextran sulfate sodium (DSS). Treatment with L. plantarum RW1 significantly increased both intestinal length and body weight compared to DSS-treated mice. 16S rRNA sequencing analysis demonstrated that L. plantarum RW1 restored the dysbiosis caused by DSS. Flow cytometry analyses further revealed that L. plantarum RW1 specifically increased regulatory T-cell proportions in Peyer's patches while reducing macrophage and neutrophil proportions, indicating the modulatory effects of L. plantarum RW1 on immune responses in gut-associated lymphatic tissue in the context of colitis. This study sheds light on the intricate interaction between probiotics and hosts, offering valuable insights into their potential application for treating inflammatory diseases in animals and humans.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Lactic Acid Bacteria Strains from Chinese Fermented Food (Suanshui) and its Protective Effect on Acute Liver Injury in Mice.","authors":"Fei Wang, Xiang Li, Qi Wang, Qian Jin, Shujie Xu, Li Tang, Zihan Zeng, Aikun Fu, Jinhui Zhu, Qiao Zhang, Rongchang Yang, Yingping Xiao, Weifen Li","doi":"10.1007/s12602-024-10408-y","DOIUrl":"https://doi.org/10.1007/s12602-024-10408-y","url":null,"abstract":"<p><p>Suanshui is a traditional fermented vegetable food in the southwest of China that is a rich source of probiotics. This study aimed to screen probiotics from Suanshui that have a protective effect against liver injury using in vivo and in vitro experiments. Eleven strains were isolated according to acid-producing capacity and probiotic properties, including antibacterial activity against pathogenic bacteria, tolerance to artificial gastrointestinal fluid and bile salts, heat resistance, antioxidant capacity, hydrophobicity, autoaggregation capacity, and adhesion capacity. Three probiotic strains, LAB36 (Lacticaseibacillus paracasei, (L. paracasei)), LAB19 (Lactiplantibacillus plantarum (L. plantarum)), and LAB51 (Limosilactobacillus fermentum (L. fermentum)), were obtained by the TOPSIS comprehensive evaluation. Furthermore, in an in vitro model of inflammation induced by LPS treatment of AML12, metabolites derived from the intestinal contents of mice treated with the three strains significantly decreased the gene expression of IL-1β, TNF-α, and Caspase 3 in the LAB (19, 36, 51) + LPS group (P < 0.05) and significantly increased the gene expression of Bcl-2 compared with those in the untreated LPS group (P < 0.05). In addition, the LAB36 + LPS group also exhibited significantly decreased gene expression of TLR4 and Bax (P < 0.05). In vivo experiments, LAB36 pretreatment significantly decreased the levels of ALT, AST, and inflammatory factors (IL-1β and IL-6) in the serum, improved the antioxidant capacity, and reduced apoptosis in the livers of the model mice (liver injury induced by D-GalN/LPS). In summary, LAB36 isolated from Suanshui has a protective effect on liver injury.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Two Bacteriocinogenic Ligilactobacillus Strain Association Inhibits Growth, Adhesion, and Invasion of Salmonella in a Simulated Chicken Gut Environment.","authors":"Saba Miri, Hebatoallah Hassan, Galal Ali Esmail, Emmanuel N Njoku, Mariem Chiba, Basit Yousuf, Tamer A E Ahmed, Maxwell Hincke, Walid Mottawea, Riadh Hammami","doi":"10.1007/s12602-023-10148-5","DOIUrl":"10.1007/s12602-023-10148-5","url":null,"abstract":"<p><p>In this study, we aimed to develop a protective probiotic coculture to inhibit the growth of Salmonella enterica serovar Typhimurium in the simulated chicken gut environment. Bacterial strains were isolated from the digestive mucosa of broilers and screened in vitro against Salmonella Typhimurium ATCC 14028. A biocompatibility coculture test was performed, which identified two biocompatible strains, Ligilactobacillus salivarius UO.C109 and Ligilactobacillus saerimneri UO.C121 with high inhibitory activity against Salmonella. The cell-free supernatant (CFS) of the selected isolates exhibited dose-dependent effects, and the inhibitory agents were confirmed to be proteinaceous by enzymatic and thermal treatments. Proteome and genome analyses revealed the presence of known bacteriocins in the CFS of L. salivarius UO.C109, but unknown for L. saerimneri UO.C121. The addition of these selected probiotic candidates altered the bacterial community structure, increased the diversity of the chicken gut microbiota challenged with Salmonella, and significantly reduced the abundances of Enterobacteriaceae, Parasutterlla, Phascolarctobacterium, Enterococcus, and Megamonas. It also modulated microbiome production of short-chain fatty acids (SCFAs) with increased levels of acetic and propionic acids after 12 and 24 h of incubation compared to the microbiome challenged with S. Typhimurium. Furthermore, the selected probiotic candidates reduced the adhesion and invasion of Salmonella to Caco-2 cells by 37-39% and 51%, respectively, after 3 h of incubation, compared to the control. These results suggest that the developed coculture probiotic strains has protective activity and could be an effective strategy to control Salmonella infections in poultry.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2021-2038"},"PeriodicalIF":5.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10111045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gum Arabic/Chitosan Coacervates for Encapsulation and Protection of Lacticaseibacillus rhamnosus in Storage and Gastrointestinal Environments.","authors":"Paloma Barajas-Álvarez, José Nabor Haro-González, Marisela González-Ávila, Hugo Espinosa-Andrews","doi":"10.1007/s12602-023-10152-9","DOIUrl":"10.1007/s12602-023-10152-9","url":null,"abstract":"<p><p>Probiotics, such as Lacticaseibacillus rhamnosus, are essential to the food industry for their health benefits to the host. The Lcb. rhamnosus strain is susceptible to processing, gastrointestinal, and storage conditions. In this study, Lcb. rhamnosus strains were encapsulated by complex coacervation in a gum arabic/chitosan or gum arabic/trehalose/chitosan and cross-linked with sodium tripolyphosphate. The physicochemical properties (zeta potential, water activity, water content, and hygroscopicity), encapsulation efficiency, and probiotic survival under storage conditions and simulated gastrointestinal fluids were evaluated. The results showed that crosslinking improves the encapsulation efficiency after drying; however, this result was remarkable when trehalose was used as a cryoprotectant. Furthermore, the encapsulation matrix preserved the viability of probiotics during 12 weeks with probiotic counts between 8.7-9.5, 7.5-9.0, and 5.2-7.4 log₁₀ CFU g^-1 at -20, 4, and 20 °C, respectively. After 12 days of digestion in an ex vivo simulator, acetic, butyric, propionic, and lactic acid production changed significantly, compared to free probiotic samples. This work shows that encapsulation by complex coacervation can promote the stability of probiotic bacteria in storage conditions and improve the viability of Lcb. rhamnosus HN001 during consumption so that they can exert their beneficial action in the organism.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2073-2084"},"PeriodicalIF":5.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}