Probiotics and Antimicrobial Proteins最新文献

{"title":"Lacticaseibacillus paracasei SX52-B Isolated from Centenarians' feces: Genomic Analysis and Hypoglycemic Activity Evaluation.","authors":"Yueting Zhu, Congjie Ning, Yushan Ding, Weiwen Xiong, Poyu Liao, Guanfu Yang, Jingzhen Wu, Peihua Tang, Xinhe Luo, Lijun Lv, Hui Luo, Yi Qi, Xuemeng Li","doi":"10.1007/s12602-026-11230-4","DOIUrl":"https://doi.org/10.1007/s12602-026-11230-4","url":null,"abstract":"<p><p>Probiotics have been reported to effectively modulate human glucose metabolism. Here, we aim to isolate and identify Lacticaseibacillus paracasei SX52-B (L. paracasei SX52-B) from the intestines of centenarians, investigate its hypoglycemic properties, and explore the potential molecular mechanisms through integration of genomic analysis. To evaluate the probiotic properties, L. paracasei SX52-B was subjected to growth characteristics, safety and functional tests. Whole-genome sequencing of L. paracasei SX52-B was performed to identify functional genes related to hypoglycemic properties. Genomic annotation was conducted by comparing against databases such as GO, COG, KEGG, and CAZy. Firstly, L. paracasei SX52-B exhibited great biosafety across a series of assays. It could survive in simulated gut microbiota juices, indicating significant tolerance to acidic conditions and bile salts. Moreover, L. paracasei SX52-B showed no hemolytic activity and exhibited antibiotic susceptibility within safe limits. In the acute oral toxicity test of mice, neither adverse reactions nor significant differences in organ indices were detected after they were fed different doses of L. paracasei SX52-B for 28 days. Genomic sequencing and analysis revealed that the genome size of SX52-B is 2,991,568 bp. Through functional annotations, relatively rare carbohydrate metabolism-related genes such as malX (maltose/glucose PTS system EIICB component), tpiA (triosephosphate isomerase) and E5.1.3.15 (glucose-6-phosphate 1-epimerase), were identified in L. paracasei SX52-B, which were rarely detected in common L. paracasei strains. These results suggest that L. paracasei SX52-B possesses distinct genomic features related to glucose-lowering pathways. On the other hand, the result of in vitro functional assay demonstrated that L. paracasei SX52-B exhibited a high α-amylase activity inhibition rate (94.67%) and favorable lipid-lowering capacity. The supernatant of L. paracasei SX52-B also exhibited significant antioxidant activity. To verify the hypoglycemic capacity of L. paracasei SX52-B in vivo, we employed a hyperglycemic zebrafish model. It is revealed that both supernatant and bacteria of L. paracasei SX52-B significantly reduced the glucose content in zebrafish (P < 0.001). The study findings highlight that L. paracasei SX52-B exhibits favorable probiotic properties and hypoglycemic capacity, holding promising potential for application in the development of functional hypoglycemic foods or pharmaceutical products.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Gut-Brain Axis and Neurodegenerative Diseases: From Mechanistic Pathways to Therapeutic Interventions.","authors":"Weilun Lin, Zhiheng Shu, Gang Liu, Jun Fang, Sujuan Ding","doi":"10.1007/s12602-026-11246-w","DOIUrl":"https://doi.org/10.1007/s12602-026-11246-w","url":null,"abstract":"<p><p>As the global population ages, the incidence of neurodegenerative diseases (NDDs) has increased. Neurodegenerative diseases have complex pathogenesis and currently lack fundamental treatment methods. The gut-brain axis (GBA) participates in the pathogenesis of NDDs through bidirectional communication between the gut microbiota and the central nervous system (CNS). This review systematically introduces the pathogenic characteristics of representative NDDs such as Alzheimer's disease (AD), Parkinson's disease (PD), and multiple sclerosis (MS), as well as the association mechanisms between these diseases and GBA. The pathogenic characteristics of NDDs are closely related to changes in the composition of the gut microbiota and differences in gut microbiota metabolites (such as short-chain fatty acids (SCFAs), neurotransmitters, tryptophan metabolites, etc.). These changes influence the CNS through multiple pathways, including neuroendocrine, vagus nerve, and immune regulation, ultimately contributing to the onset and progression of the diseases. Furthermore, we have collected and summarized preclinical and clinical evidence from recent years regarding therapeutic interventions based on the GBA mechanism, including probiotics, prebiotics, and fecal microbiota transplantation (FMT). Nevertheless, major knowledge gaps remain, particularly regarding causality between gut microbiota changes and NDDs, the underlying molecular mechanisms, and the clinical efficacy of GBA-targeted therapies. This article aims to review the mechanisms of the interaction between the gut microbiota and the brain in neurodegenerative diseases, and based on the existing experimental evidence, provide theoretical basis and future development directions for future prevention and treatment strategies targeting GBA.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Cationic Antimicrobial Peptide Scymidgutin_8-29 from Scylla paramamosain with Antimicrobial and Anti-inflammatory Activities and Therapeutic Potential in DSS-induced Colitis in Mice.","authors":"Chang Zhang, Xianxian Dong, Ning Ding, Fangyi Chen, Ke-Jian Wang","doi":"10.1007/s12602-026-11247-9","DOIUrl":"https://doi.org/10.1007/s12602-026-11247-9","url":null,"abstract":"<p><p>Marine-derived antimicrobial peptides possess both antibacterial and immunomodulatory activities, offering potential advantages for treating complex inflammatory conditions. Meanwhile, the management of inflammatory bowel disease is complicated by the interplay of microbial dysbiosis and bacterial-driven inflammation, which conventional single-target therapies frequently fail to address. In this study, we identified a novel cationic antimicrobial peptide, Scymidgutin_8-29, from the mud crab Scylla paramamosain. This 22-residue peptide possesses a calculated molecular mass of 2658.23 Da, an estimated pI of 12.31, and 37.5% hydrophobicity. Scymidgutin_8-29 exhibited potent, broad-spectrum membrane-disrupting activity against clinically relevant pathogens, including multidrug-resistant strains, with MBCs of 1.5-3 µM against the tested multidrug-resistant Acinetobacter baumannii isolates and 6-12 µM against MRSA isolates, achieving > 99.9% killing within 20 min. Furthermore, the peptide demonstrated potent anti-inflammatory activity by promoting M2 macrophage polarization in LPS-stimulated RAW264.7 cells, as evidenced by the downregulation of iNOS (to 0.24-fold of LPS control), NO (to 0.47-fold), and pro-inflammatory cytokines (IL-1β, TNF-α, IL-6 to 0.67-0.73-fold), along with the upregulation of Arg-1 (to 1.80-fold) and IL-10 (to 1.38-fold). In a dextran sulfate sodium (DSS)-induced acute colitis mouse model, intrarectal administration of Scymidgutin_8-29 (5 mg/kg) significantly reduced the disease activity index (from 7.0 to 3.0), restored colonic histopathology, rebalanced cytokine profiles, and altered gut microbiota composition, notably decreasing colitis-associated Akkermansia and Proteobacteria. These results indicate that Scymidgutin_8-29 possesses both direct membrane-disruptive antibacterial activity and immunomodulatory capacity, and demonstrates therapeutic efficacy in the DSS-induced colitis model, positioning it as a potential multi-functional therapeutic candidate for intestinal inflammation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technological Potential of Native Lactic Acid Bacteria from Brazilian Canastra Cheese: Safety and Functional Characterization.","authors":"Gabriela Zampieri Campos, Elionio Galvão Frota, Gustavo Augusto Lacorte, Ricardo Pinheiro de Souza Oliveira, Svetoslav Dimitrov Todorov, Uelinton Manoel Pinto","doi":"10.1007/s12602-026-11233-1","DOIUrl":"https://doi.org/10.1007/s12602-026-11233-1","url":null,"abstract":"<p><p>Canastra artisanal Minas cheese is a traditional Brazilian raw-milk cheese whose fermentation relies on \"pingo\", an endogenous whey culture that harbors autochthonous lactic acid bacteria (LAB) with technological relevance. This study evaluated the safety and technological properties of LAB isolated from pingo and cheeses from Serra da Canastra region, Brazil. Among 199 presumptive LAB isolates screened for hemolytic activity, mucin degradation, and antibiotic susceptibility, 116 met the preliminary safety criteria. Rep-PCR and partial 16S rRNA gene sequencing revealed 16 genetic groups, mainly represented by Streptococcus lutetiensis (32%), Lactiplantibacillus plantarum (28%), Lpb. paraplantarum (14%), Lactococcus lactis (12%), and Leuconostoc mesenteroides (12%). Vancomycin resistance genes were not detected, whereas biogenic amine-related genes were identified in some strains, and Enterococcus durans 14A3 produced tyramine in vitro. Technological characterization showed variability in acidification capacity (ΔpH/t24 = 0.010-0.061), proteolytic and lipolytic activities, diacetyl production and enzymatic profiles. Genes associated with bacteriocin production, adhesion, and γ-aminobutyric acid (GABA) synthesis were also detected in selected strains. The integration of safety and technological traits identified Lpb. plantarum 2B3 and L. lactis 6D5 as the most promising candidates, combining favorable safety profiles with consistent technological characteristics. These findings highlight the potential of the autochthonous microbiota of Canastra artisanal Minas cheese as a source of adjunct cultures for artisanal dairy production, while reinforcing the importance of strain-level safety assessment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus Plantarum CKDB008 and Lacticaseibacillus Rhamnosus LDTM7511 Attenuate Visceral Hypersensitivity and Suppress Serum IgE in an Acetic Acid-Induced IBS-Like Rat Model.","authors":"Ji-Hee Kim, Soo Je Park, Chul Sung Huh, Chang Hun Shin","doi":"10.1007/s12602-026-11238-w","DOIUrl":"https://doi.org/10.1007/s12602-026-11238-w","url":null,"abstract":"<p><p>Irritable bowel syndrome (IBS) is a disorder of gut-brain interaction characterized by visceral hypersensitivity, impaired intestinal barrier, immune activation, and gut microbiota dysbiosis, for which standardized treatment remains limited. In this study, we evaluated Lactiplantibacillus plantarum CKDB008 (LP) and Lacticaseibacillus rhamnosus LDTM7511 (= CKDB511; LR), each administered at low (1 × 10⁸ colony forming unit [CFU]/day) and high (1 × 10⁹ CFU/day) doses for 14 days in an acetic acid-induced post-inflammatory IBS-like rat model. Visceral hypersensitivity was assessed by the abdominal withdrawal reflex (AWR) test, and serum and distal colonic markers of stress, immunity, barrier function, and gut microbiota were analyzed. LR consistently attenuated visceral hypersensitivity at both doses, whereas LP reduced serum cortisol more strongly at the high dose, indicating distinct response profiles across endpoints. Serum IgE was significantly reduced in all probiotic-treated groups regardless of strain or dose (P < 0.01), and distal colonic zonula occludens-1 expression was upregulated primarily in the LR-treated groups, reflecting limited and marker-specific modulation of barrier-related readouts. In the gut microbiota, Kineothrix alysoides was significantly depleted in the low-dose LP group after false discovery rate correction (Q < 0.05), with a positive correlation observed between its abundance and AWR area under the curve. Predicted microbial pathway profiles also showed changes primarily in the LR-treated groups relative to IBS at the suggestive level. Collectively, these findings provide preliminary preclinical evidence that LP and LR alleviate several IBS-related phenotypes in this rat model, supporting their further evaluation as candidate probiotic strains for IBS.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Protein and Lipid Cargo Sorting into Extracellular Vesicles of Lacticaseibacillus paracasei subsp. paracasei.","authors":"Huixue Hu, Ming Yang, Jiamu Kang","doi":"10.1007/s12602-026-11239-9","DOIUrl":"https://doi.org/10.1007/s12602-026-11239-9","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are nanoscale lipid bilayer particles that transport bioactive cargo. EVs from lactic acid bacteria show health benefits and drug-delivery potential, yet cargo encapsulation remains understudied. Here, EVs were isolated, purified, and characterized from the bacteriocin-producing strain Lacticaseibacillus paracasei subsp. paracasei YCL29, which was isolated from fermented Yucha. Proteomic and lipidomic analyses were subsequently conducted to compare the EVs with their parental L. paracasei YCL29 cells. Nanoflow cytometry indicated EVs with mean diameter 86.0 ± 18.8 nm at 4.83 × 10⁹ particles/mL. Proteomic analysis identified 2,120 high-confidence proteins and revealed selective cargo sorting, with 313 proteins enriched in EVs and 446 downregulated relative to the parent cells. EV-enriched proteins were associated with stress tolerance, nutrient transport/metabolism, nucleic acid metabolism, catalysis, and antibiotic biosynthesis, including cold-shock and antioxidant proteins were detected under non-stress conditions. Lipidomic analysis showed enrichment of phosphatidylethanolamine and triglycerides, defining a distinct EV lipid signature. These findings provide insights into potential cargo-sorting mechanisms and functions of L. paracasei EVs.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faecalibacterium prausnitzii: Mechanistic Insights and Therapeutic Potential in Host-Microbe Crosstalk.","authors":"Wenfei Qin, Guangqiang Wang, Yang Fu, Yue Zeng, Yongjun Xia, Yi Sheng, Lianzhong Ai","doi":"10.1007/s12602-026-11236-y","DOIUrl":"https://doi.org/10.1007/s12602-026-11236-y","url":null,"abstract":"<p><p>Faecalibacterium prausnitzii (Fp), a potential candidate for a next-generation, is widely distributed in the feces and intestinal tract of mammals. Accumulating evidence indicates that Fp and its metabolites exert anti-inflammatory, immunomodulatory, and maintain intestinal barrier integrity. In this review, we summarize the biological characteristics of Fp and its nutritional cross feeding interactions with other microbial species. We further synthesize current evidence on the local roles of Fp in intestinal diseases and systemic effects on distant organs via the gut-organ axis. Furthermore, we outline the mechanisms by which its effector molecules, including butyrate, microbial anti-inflammatory molecule (MAM), and extracellular vesicles (EVs), contribute to disease amelioration. Finally, we discuss the current challenges and future perspectives for Fp. Collectively, as an important commensal bacterium in the gut, Fp provides a theoretical basis for the development of novel functional microorganisms with probiotic properties.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational Prioritisation of Fish-Derived Antimicrobial Peptides by Integrated Multi-Layer Immunogenicity and Structural Analysis.","authors":"Akshay Sasidharan, Ananya Pandey, Shreya Nandi Roy, Vinutha Anandhan Sujatha, Kanishk Prabhu, Rajasekaran Ramalingam","doi":"10.1007/s12602-026-11240-2","DOIUrl":"https://doi.org/10.1007/s12602-026-11240-2","url":null,"abstract":"<p><p>Fish-derived antimicrobial peptides (AMPs) represent a promising but underexplored source of therapeutic candidates. However, their immunological safety for potential human application has not been systematically evaluated. Here, we applied a multi-stage computational approach to 154 fish AMPs retrieved from the Antimicrobial Peptide Database (APD6). Sequential safety filtering reduced the dataset to 23 AMP candidates, which were subsequently evaluated using a composite safety and immunogenicity risk score based on MHC class I binding, MHC class II binding, B-cell epitope exposure, and residual hemolytic risk. B-cell risk was conditioned on MHC class II activation to account for the role of CD4⁺ T-cell help in class-switched antibody responses. Eight AMPs were classified as Predicted Low Risk based on their relative composite safety and immunogenicity risk. Both this classification and the rank order of the three highest-ranked candidates remained stable across the tested sensitivity scenarios. The eight Predicted Low Risk candidates were further evaluated for conformational variability, secondary structure, structural rigidity, and membrane interaction. NRC-01 showed the lowest composite safety and immunogenicity risk score (0.213), indicating the lowest predicted risk among the evaluated candidates. NRC-18 achieved the highest final multi-criteria score (0.764), combining a relatively low composite safety and immunogenicity risk score (0.296), strong predicted membrane interaction (ΔG = -5.2 kcal/mol), high helical content (69.0%), and the highest dominant cluster population among the top three candidates (57.86%). Overall, the combined computational analyses identified a small group of fish-derived AMPs with favourable predicted safety, immunogenicity, and structural characteristics that warrant further experimental investigation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SmartVax-Bp: An Immunoinformatic guided Multi-Epitope Vaccine Blueprint Against Burkholderia pseudomallei.","authors":"Mohammad Abdullah Aljasir, Dalia M Alammari, Nawal Helmi, Saira, Sajjad Ahmad, Dong-Qing Wei","doi":"10.1007/s12602-026-11241-1","DOIUrl":"https://doi.org/10.1007/s12602-026-11241-1","url":null,"abstract":"<p><p>Burkholderia pseudomallei (B. pseudomallei) is the causative agent of melioidosis, a potentially serious and life-threatening infectious disease in humans. This pathogen invades the bloodstream and infects multiple organs, including the lungs, skin, liver, spleen, bones, and brain. The enzymes polyphosphate kinase 1 (Ppk1), type III secretion system cluster 3 (TTSS3)-effector protein (BopA), and trehalose-phosphate phosphatase (TPP) are vital for the survival and pathogenicity of B. pseudomallei, playing central roles in oxidative phosphorylation, stress responses, metabolism, biofilm formation, autophagy evasion, and motility. The normal function of these enzymes enables B. pseudomallei to produce energy and sustain fatal infections in affected organs. Due to their critical roles, these enzymes were identified as key targets for multi-epitope vaccine (MEV) design, with the aim of suppressing their expression in infected individuals by provoking specific immune responses. After retrieving the virulence factors Ppk1, BopA, and TPP, they were processed for B-cell and T-cell epitope prediction. Afterwards, predicted epitopes were filtered based on acceptable properties, including antigenicity, allergenicity, solubility, and toxicity. The filtered epitopes were linked using specific linkers and an adjuvant to create a finalized MEV construct. The structure of MEV was predicted and refined, demonstrating significant stability with a verify3D score of 90.28%. The MEV was further docked with immune receptors MHC-I, MHC-II, and TLR4, showing binding potentials of -1191.5 Kcal/mol, -1131.7 Kcal/mol, and - 1135.7 Kcal/mol, and maximum interactions, respectively. Molecular dynamics simulations of the MEV-docked complexes revealed a significant increase in B-factor and lower eigenvalues, indicating favorable and high-affinity interactions with the immune receptors. Furthermore, immune simulation analyses demonstrated a substantial production of immunoglobulins against the MEV, confirming its potential as an ideal antigen with low toxicity, optimal solubility, and no allergenicity. Based on these promising properties, the MEV was virtually cloned into a pET-28a(+) vector and expressed in Escherichia coli (E. coli). This suggests that the data can be used by experimentalists to produce the MEV in the lab and make it available to the community after clinical trials.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediococcus acidilactici ZQ-19 Ameliorates Ulcerative Colitis via Remodeling Gut Microbiota and Glutathione Redox Homeostasis.","authors":"Yimeng Lu, Qi Liu, Hu Chen, Cunhui Ding, Mengyan Chen, Qian Tian, Xusheng Guo, Zitong Ding","doi":"10.1007/s12602-026-11187-4","DOIUrl":"https://doi.org/10.1007/s12602-026-11187-4","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is closely linked to intestinal oxidative stress, gut dysbiosis and selenium metabolism disorders. In this study, 449 lactic acid bacteria strains were isolated from yak yogurt on the Qinghai-Tibet Plateau. After H₂O₂ tolerance screening, 11 highly antioxidant strains were obtained, among which Pediococcus acidilactici ZQ-19 exhibited the highest intracellular GPx activity, outstanding free radical scavenging capacity and excellent probiotic characteristics. In DSS-induced UC mice, ZQ-19 significantly alleviated weight loss, colon shortening and mucosal damage, restored tight junction protein expression, and protected liver and kidney functions. 16 S rRNA sequencing revealed that ZQ-19 was associated with reshaped gut microbiota, enriched butyrate-producing taxa and reduced pro-inflammatory pathogens; such microbial shifts were positively correlated with elevated intestinal GPx activity. Mechanistically, ZQ-19 intervention coincided with enhanced intestinal ROS clearance, altered expression of GR/GST/Trx-1, and a shifted selenoprotein profile favouring GPx isoforms by upregulating colonic SELENOP and GPx1-4 while downregulating DIO1/2. It synergistically activated Nrf2/p53 protective pathways and inhibited the MyD88/COX-2/NOX1/2 pro-oxidative inflammatory axis, with superior local regulation to mesalazine. In conclusion, ZQ-19 ameliorates UC in association with the microbiota-GPx axis and dual pathways, offering a novel probiotic intervention strategy.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}