Probiotics and Antimicrobial Proteins最新文献

{"title":"Intestinal Microbiomics and Liver Metabolomics Insights into the Improvement Effects of Lactiplantibacillus plantarum FJG2627 on Lipopolysaccharide-Induced Acute Liver Injury in Mice.","authors":"Siyu He, Yuheng Yang, Li Ni, Xucong Lv, Weiling Guo, Zhifeng Cai, Youting Chen","doi":"10.1007/s12602-026-11045-3","DOIUrl":"https://doi.org/10.1007/s12602-026-11045-3","url":null,"abstract":"<p><p>Endotoxin is one of the chemical substances that cause acute liver injury (ALI), which become a common health problem worldwide. Lactic acid bacteria (LAB) have emerged as a promising candidate for attenuating endotoxin-induced ALI. Lactiplantibacillus plantarum FJG2627 is a LAB isolated from traditional pickles with excellent probiotic effects. In this study, the protective functions of L. plantarum FJG2627 against ALI caused by lipopolysaccharides (LPS) in mice were investigated. Administration of L. plantarum FJG2627 significantly reduced serum ALT, AST, and pro-inflammatory factors (IL-1β and IL-6), while markedly increased the anti-inflammatory factor (IL-10). Additionally, administration of L. plantarum FJG2627 increased the abundance of beneficial bacteria such as Adlercreutzia, and reduced that of pathogenic bacteria such as Bilophila. Non-targeted metabolomics revealed that L. plantarum FJG2627 intervention altered metabolic pathways related to glucose (including galactose metabolism, etc.) and amino acid metabolisms (including histidine metabolism, etc.) in ALI mice. In summary, L. plantarum FJG2627 exerts a significant protective effect on LPS-induced ALI by controlling oxidation, suppressing inflammatory mediators, modulating intestinal microbial communities, and liver metabolites. L. plantarum FJG2627 has the potential to be developed as a food-grade Lactobacillus for safeguarding against liver damage.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum Mediated Tryptophan Metabolism to Alleviate Tropomyosin-induced Food Allergy.","authors":"Jing Ma, Pengyan Tong, Kang Yang, Yining Jia, Zhen Liu, Mengya Zhao, Jing Liu, Fangyu Long","doi":"10.1007/s12602-026-11039-1","DOIUrl":"https://doi.org/10.1007/s12602-026-11039-1","url":null,"abstract":"<p><p>Food allergy (FA) has become increasingly prevalent, affecting daily life. Probiotics alleviate FA by modulating immune-microbiome interactions. Nevertheless, the key metabolites and mechanisms by which L. plantarum alleviates FA remain unclear. In this study, L. plantarum demonstrated the ability to improve FA by modulating regulatory T/Th1/Th2 balance, modulating gut microbial composition, and regulating gut metabolites. Tropomyosin sensitization was associated with decreased levels of 5-hydroxyindole-3-acetic acid (5-HIAA) in cecum contents, a phenomenon also observed in the serum of FA mice and patients. In vitro experiments showed that tryptophan (Trp), tryptamine, tryptophol, kynurenine, 5-HIAA, and indole-3-acetamide inhibited RBL-2H3 cell degranulation; however, this inhibitory effect was attenuated by the aryl hydrocarbon receptor (AhR) antagonist CH223191. Moreover, in vivo results indicated that dietary supplementation with 5-HIAA or Trp downregulated IgE and cytokine levels in an AhR-dependent manner. This study provided evidence for the positive role of Trp metabolites in alleviating FA.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Role of Probiotics in the Prevention and Treatment of Gastrointestinal Cancer.","authors":"Sadegh Zomorodimanesh, Seyed Hadi Razavi, Parivash Zomorodimanesh","doi":"10.1007/s12602-026-11040-8","DOIUrl":"https://doi.org/10.1007/s12602-026-11040-8","url":null,"abstract":"<p><p>Probiotics, live microorganisms with health-promoting properties, play a vital role in modulating the gut microbiota and enhancing immune responses. Their potential for regulating key signaling pathways, including NF-κB, PI3K/AKT, and Wnt/β-Catenin, suggests promise in cancer prevention as they can mitigate inflammation, strengthen intestinal barriers, and influence cytokine production, offering multiple therapeutic benefits. This review highlights the mechanisms by which probiotics may contribute to gastrointestinal cancer prevention and treatment, discussing their capacity to regulate inflammatory cytokines, enhance epithelial integrity, and balance gut microbiota. Evidence from preclinical and a growing number of clinical studies is summarized. Additionally, challenges such as strain specificity, dose optimization, and safety evaluations are analyzed alongside potential integration into conventional cancer therapies. Current data indicate that probiotics hold potential as complementary agents in oncology, possibly capable of inhibiting tumor growth, enhancing immune surveillance, and improving the efficacy of existing treatments. Future research should prioritize robust, long-term clinical trials and molecular studies to optimize their application in cancer management.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective Mechanisms of Lactiplantibacillus plantarum SCS7 Cell-Free Extract Against Aflatoxin B1 Toxicity.","authors":"Yuxi Wang, Wei Pu, Ying Zhang, Qiuyan Liu, Xiao Meng, Lishi Jiang","doi":"10.1007/s12602-026-11034-6","DOIUrl":"https://doi.org/10.1007/s12602-026-11034-6","url":null,"abstract":"<p><p>Aflatoxin B1 (AFB1) is a widespread contaminant of food and feed that poses significant risks to both human and animal health. Developing safe and effective strategies to mitigate its toxicity remains a major public health priority. In this study, we investigated the protective effects of Lactiplantibacillus plantarum SCS7 cell-free extract (CFE) against AFB1-induced liver injury using integrated in vivo (mouse) and in vitro (AML12 hepatocyte) models. CFE treatment markedly alleviated AFB1-induced hepatic damage, mitochondrial dysfunction, and gut microbiota dysbiosis. Mechanistic analyses indicate that the protective effects of CFE are associated with the alleviation of excessive mitophagy and concomitant restoration of the PINK1/Parkin signaling axis, while systemic protection is conferred through modulation of the gut-liver axis. Importantly, unlike live probiotics, CFE circumvents concern related to intestinal colonization and biosafety while maintaining multi-pathway efficacy. Notably, this study identifies a previously unrecognized role for probiotic-derived metabolites in organelle quality control, demonstrating that CFE mitigates liver injury by inhibiting pathological mitophagy. Furthermore, CFE reshaped the intestinal microbiota and promoted the production of beneficial microbial metabolites, changes that were closely associated with improved hepatic homeostasis. Collectively, these findings suggest a coordinated interplay among the gut microbiota, microbial metabolites, and restoration of the PINK1/Parkin pathway, thereby providing a theoretical foundation for the development of stable, cell-free probiotic therapeutics with potential applications in food safety and public health.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Efficacy of Recombinant Endolysin S.AgEndo1332 in Combination with Antibiotics against Mastitis-Associated S. aureus in a Mouse Mastitis Model.","authors":"Richa Sarkar, Ravi Kant Agrawal, Abhilash S Goud, Thanksy S Akkara, Kajal Bankoti, Keshav Kumar, Mrinalini Saini, Jaideep Singh, Gaurav Kumar Sharma, Vidya Singh, Deepak Kumar, Bhoj Raj Singh","doi":"10.1007/s12602-026-11033-7","DOIUrl":"https://doi.org/10.1007/s12602-026-11033-7","url":null,"abstract":"<p><p>Bovine mastitis is one of the diseases that affect cattle and has a considerable detrimental impact on both the economics and animal welfare internationally. S. aureus is among the most relevant causative agents of this disease. Due to developing bacterial resistance and public health regulations, the number of antibiotics to treat mastitis is restricted. Endolysins, cell-wall-degrading enzymes of bacteriophage origin, are emerging as a promising alternative to traditional antibiotics. Moreover, combination therapy is frequently utilized to combat drug-resistant bacterial pathogens. In this study, we evaluated the therapeutic efficacy of the recombinant endolysin S.AgEndo1332 in combination with the antibiotic gentamicin. The synergistic activity of this combination was assessed in vitro using checkerboard and time-kill assays, and in vivo using a mouse mastitis model induced by mastitogenic S. aureus. S.AgEndo1332, a homolog of GBS phage B30 lysin gene, was custom synthesized, expressed by prokaryotic expression system, and purified under native purification protocol. S.AgEndo1332 showed strong antimicrobial activity against Streptococcus agalactiae (MIC 25 µg/mL) and S. aureus (MIC 50 µg/mL) strains in Tris-HCl buffer system at pH 7.5. The lytic activity of S.AgEndo1332 wasretained at -80 °C for 12 weeks. The combination of S.AgEndo1332 (12.5 µg/mL) with gentamicin (0.0594 µg/mL) was showing synergistic activity by checkerboard assay with FIC index 0.375. The dosage for the combination treatment (10X concentration, S.AgEndo1332: 125 µg/mL and gentamicin: 0.594 µg/mL) used for the in vivo trial was determined based on cell viability by the MTT assay (> 93% cell viability), time kill kinetics study (30 min) and lytic activity in milk (within 30 min). In S. aureus induced-mouse mastitis model, combination therapy significantly reduced the intramammary bacterial load and result was further supported by consistent findings of expression of pro and anti-inflammatory cytokine genes (TNF-α, IL-6, IFN-γ, and IL-10), and histopathological analysis of the mammary gland tissue section. Overall, our findings highlight the potential of combining endolysin with antibiotics as a promising alternative treatment for S. aureus-induced bovine mastitis.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Evaluation of Safety and Probiotic Potential of Lactiplantibacillus plantarum HY7715 Isolated from Korean kimchi.","authors":"Ju-Eun Lee, Tae-Hyun Lim, Ji-Hyun Kim, Yong-Tae Kim, Jae Jung Shim, Jae-Hwan Lee","doi":"10.1007/s12602-026-10972-5","DOIUrl":"https://doi.org/10.1007/s12602-026-10972-5","url":null,"abstract":"<p><p>Lactiplantibacillus plantarum HY7715, isolated from Korean kimchi, has been previously reported to overproduce riboflavin in vitro and in vivo, indicating its functional potential as a probiotic candidate. Given this characteristic, a comprehensive safety and genomic evaluation is required prior to application. In this study, we assessed the safety, genomic stability, and probiotic-related properties of HY7715 through whole-genome sequencing, comparative genomics, and phenotypic analyses. The genome (3,348,486 bp; 44.29% GC content) was assembled into seven contigs and encoded 3,224 protein-coding sequences, of which 2,933 functionally annotated. No virulence factors or acquired antimicrobial resistance genes were detected, and mobile genetic elements did not harbor undesirable determinants. Although phenotypic resistance to kanamycin was observed, the absence of corresponding resistance genes indicates intrinsic resistance. HY7715 also exhibited γ-hemolysis and lacked genes associated with cytotoxic secondary metabolites or biogenic amine production. The rib operon was identified in the genome, and in silico analysis of the FMN riboswitch region revealed a unique nucleotide substitution potentially affecting regulatory conformation, which may contribute to the riboflavin-overproducing phenotype. Comparative pangenome analysis of 60 L. plantarum genomes revealed that HY7715 shares a conserved genomic backbone dominated by core and soft-core genes. Genes associated with stress tolerance, adhesion, carbohydrate utilization, and bacteriocin production were identified, consistent with probiotic-related traits. Collectively, these findings indicate that HY7715 is a genomically stable and safe strain with regulatory features supporting its functional probiotic potential.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome-Modulating Strategies in Anticancer Therapy: A Review of Current Evidence and Recommendations for Further Treatment Improvement.","authors":"D Böhm, H J Guchelaar, M L Smidt, M J Deenen","doi":"10.1007/s12602-026-11024-8","DOIUrl":"https://doi.org/10.1007/s12602-026-11024-8","url":null,"abstract":"","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Survival of Lactiplantibacillus plantarum TUA2424L in Acidic Environments Using Different Types of Protectants in Freeze-Dried Powder.","authors":"Akinori Uehara, Mayumi Maekawa, Kazuki Nakagawa","doi":"10.1007/s12602-026-11036-4","DOIUrl":"https://doi.org/10.1007/s12602-026-11036-4","url":null,"abstract":"","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-Triggered Microcapsules for Colon-Targeted Delivery of Lacticaseibacillus rhamnosus Improve Metabolic Parameters in an Obese Mouse Model.","authors":"Qikun Cheng, Hengrui Xu, Lijia Shi, Shuyi Li, Shuhua Liu, Wenjing Chen, Dong Ma","doi":"10.1007/s12602-026-11035-5","DOIUrl":"https://doi.org/10.1007/s12602-026-11035-5","url":null,"abstract":"<p><p>Obesity is associated with numerous metabolic disorders, and identifying safe and effective strategies for obesity management remains an important research priority. In our previous research, we successfully developed a colon-targeted probiotics oral delivery system, AP_2.0, which showed potential for obesity intervention. In this study, Lacticaseibacillus rhamnosus (L. rhamnosus) was encapsulated within AP_2.0, and the formulation was tested in a mouse model of obesity. The oral administration of AP_2.0@ L. rhamnosus resulted in a 14.6% reduction in body weight gain and a 20% decrease in low-density lipoprotein cholesterol compared to the model group, paralleling the metabolic improvements observed with orlistat in this murine model. 16S rRNA sequencing revealed that oral administration of AP_2.0@L. rhamnosus increased the abundance of Streptococcus_acidominimus and Ruminococcaceae, whereas the Orlistat group showed an increase in Bifidobacterium, suggesting distinct gut microbiota modulation profiles associated with weight loss. In conclusion, AP_2.0@L. rhamnosus shows promise as a potential intervention for obesity management, although further preclinical and clinical validation is required.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Therapeutic Potential of Probiotic Clostridium butyricum and Curcuma longa against Salmonella Infection.","authors":"Yusra M Muhsin, Mahde Saleh Assafi, Sozan Mosa Sharo, Oday Mazin Abdulmunem, Huda Z Majeed, Manar Munim, Aya Fayadh","doi":"10.1007/s12602-026-11042-6","DOIUrl":"https://doi.org/10.1007/s12602-026-11042-6","url":null,"abstract":"<p><p>Salmonella is the leading cause of diarrhoeal diseases, with its incidence and severity having increased significantly. Salmonellosis is one of the most common and widely distributed foodborne diseases, resulting in thousands of deaths. The resistance of Salmonella to a variety of antibiotics has become an important public health problem throughout the world. Therefore, it is imperative to find novel antimicrobial compounds from natural sources. To date, no study has examined the synergistic effects of Clostridium butyricum probiotics and Curcuma longa against Salmonella infection. Hence, this study investigates the combined effect of the Clostridium butyricum probiotic cell-free supernatant and the crude extract of Curcuma longa plant powder (Turmeric) against Salmonella typhi. A suspension of S. typhi (10⁸ and 10⁹ CFU/ml) and 500 mg of C. longa (Turmeric) powder was used for oral administration for five groups of BALb/C mice, five per cage. Group 1 (positive control) received Salmonella only. Group 2 (negative control) received nothing. Group 3 received Salmonella plus chemical treatment. Group 4 received Levofloxacin plus natural treatment (probiotic + Curcuma longa). Group 5 received natural treatment only. Intestinal viable counts of S. typhi and probiotics were determined. Histopathological evaluation was performed by dissecting the liver and intestine. Additionally, an immunological study was conducted by measuring interleukin-10 and interferon-γ in the blood of the tested mice. The results showed that the combination of cell-free supernatant of Clostridium butyricum and C. longa extract exhibited enhanced inhibitory effects on S. typhi growth compared to either treatment alone. Also, the enhanced effect in vivo decreased the number of S. typhi and increased the animal body weight. Furthermore, the combinatorial effect demonstrated a reduction in tissues in the livers and intestinal tissues of the tested animals. Immunologically, interleukin-10 increased while interferon-γ levels decreased in groups receiving probiotics with C. longa. The current study demonstrates that probiotic Clostridium and C. longa enhance tissue repair and ameliorate infection-induced damage in mice. These findings highlight the potential of probiotic Clostridium butyricum and C. longa as immune modulators and alternative therapeutic agents, though further mechanistic and clinical studies are required to confirm their applicability.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}