Probiotics and Antimicrobial Proteins最新文献

Therapeutic Potential of Alginate-Pectin-Chitosan Encapsulated Pediococcus acidilactici Against Bile Duct Ligation-Induced Hepatic Fibrosis in Rats. 海藻酸盐-果胶-壳聚糖包封的酸化Pediococcus对大鼠胆管结扎性肝纤维化的治疗作用。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-27 DOI: 10.1007/s12602-025-10625-z

Sahar Ahmadi Asouri, Mitra Motallebi, Merat Karimi, Maryam Akhavan Taheri, Esmat Aghadavod, Mahla Qanbari, Hamed Mirzaei, Siavash Amiri, Mohammad Esmaeil Shahaboddin

{"title":"Therapeutic Potential of Alginate-Pectin-Chitosan Encapsulated Pediococcus acidilactici Against Bile Duct Ligation-Induced Hepatic Fibrosis in Rats.","authors":"Sahar Ahmadi Asouri, Mitra Motallebi, Merat Karimi, Maryam Akhavan Taheri, Esmat Aghadavod, Mahla Qanbari, Hamed Mirzaei, Siavash Amiri, Mohammad Esmaeil Shahaboddin","doi":"10.1007/s12602-025-10625-z","DOIUrl":"https://doi.org/10.1007/s12602-025-10625-z","url":null,"abstract":"Probiotic supplementation can mitigate hepatic injury, but the low survival rate of probiotics through the gastrointestinal tract limits their therapeutic efficacy. This study aimed to enhance the viability of Pediococcus acidilactici (P. acidilactici) by encapsulating it in an alginate-pectin matrix coated with chitosan (APC) and to evaluate the hepatoprotective effects of the encapsulated probiotic in a rat model of bile duct ligation (BDL)-induced hepatic fibrosis. APC microcapsules were fabricated using an extrusion technique and characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) to confirm successful encapsulation. The survival of encapsulated and non-encapsulated P. acidilactici was assessed in simulated gastrointestinal fluids. For in vivo evaluation, forty-eight rats were randomly assigned to six groups and treated for 28 days. Outcomes included serum bilirubin and liver enzyme levels, hepatic oxidative stress markers, histopathological changes, and expression of fibrosis- and inflammation-related genes measured by quantitative reverse transcription PCR (RT-qPCR). Encapsulation significantly improved probiotic viability in gastrointestinal conditions (5.15 ± 0.21 vs. 2.05 ± 0.35 log₁₀ CFU/mL after two hours, P ≤ 0.05). In BDL rats, treatment with encapsulated P. acidilactici led to significant reductions in serum bilirubin, liver enzymes, and oxidative stress, alongside improved liver histology and favorable modulation of gene expression compared to controls (P ≤ 0.05). These findings demonstrate that APC encapsulation enhances the gastrointestinal stability of P. acidilactici and supports its therapeutic potential against hepatic fibrosis.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety Assessment of GABA-Producing Levilactobacillus brevis LAB6 MTCC 25662 and Its Anti-inflammatory Effects in Murine Macrophages. 产gaba短乳酸杆菌LAB6 MTCC 25662的安全性评价及其对小鼠巨噬细胞的抗炎作用

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-27 DOI: 10.1007/s12602-025-10628-w

Tushar Matta, Kushhagra Agrawal, Priyanshi Datta, Laxmi Kumari, Mahendra Bishnoi, Kanwaljit Chopra, Kanthi Kiran Kondepudi

{"title":"Safety Assessment of GABA-Producing Levilactobacillus brevis LAB6 MTCC 25662 and Its Anti-inflammatory Effects in Murine Macrophages.","authors":"Tushar Matta, Kushhagra Agrawal, Priyanshi Datta, Laxmi Kumari, Mahendra Bishnoi, Kanwaljit Chopra, Kanthi Kiran Kondepudi","doi":"10.1007/s12602-025-10628-w","DOIUrl":"https://doi.org/10.1007/s12602-025-10628-w","url":null,"abstract":"To ascertain safety, the assessment of bacterial strains to be used as probiotics needs rigorous and well-designed in vitro and in vivo studies. Here, the safety of a GABA-producing Levilactobacillus brevis LAB6 MTCC 25662 was assessed using in (silico, vitro and vivo) approaches. Firstly, the genomic analysis suggested that LAB6 is non-pathogenic to humans, as it does not harbour the genes for virulence, pathogenesis-related and horizontally transferable antimicrobial resistance. LAB6 neither produces biogenic amines nor degrades mucin, has no haemolytic activity and does not exert cytotoxicity on HEK-293 cells. In vivo safety of LAB6 was assessed in acute, subacute and sub-chronic oral feeding experiments following revised OECD guidelines 425, 407 and 408, respectively. Histopathological, blood biochemical, haematological parameters, gut permeability and oxidative stress levels were assessed. In vivo studies indicated that LAB6 did not negatively impact haematological markers or cause deleterious histological alterations in the vital organs. The anti-inflammatory potential of LAB6 in alleviating lipopolysaccharide (LPS)-induced inflammation in murine macrophages was assessed in the presence of GABAA and GABAB receptor antagonists. LAB6, owing to its GABA-producing ability, prevented LPS-induced inflammation by reducing TNF-α, IL-6 and IL-1β levels by 62.3%, 27.2% and 74.8%, respectively. Antagonism of the GABAA receptor with bicuculline methiodide (BMI) partially blunted the protective effects of LAB6, while GABAB antagonism has no significant impact in curtailing its protective effects. Overall results indicated that oral consumption of anti-inflammatory and GABA-producing LAB6 is safe to test in human studies further.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Interventions: Postbiotics and Psychobiotics in Neurodegenerative Disease Treatment. 创新干预：神经退行性疾病治疗中的后生物和精神生物。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-27 DOI: 10.1007/s12602-025-10632-0

Mandeep Kumar Gupta, Kanupriya Chauhan, Snigdha Bhardwaj, Rajnish Srivastava

{"title":"Innovative Interventions: Postbiotics and Psychobiotics in Neurodegenerative Disease Treatment.","authors":"Mandeep Kumar Gupta, Kanupriya Chauhan, Snigdha Bhardwaj, Rajnish Srivastava","doi":"10.1007/s12602-025-10632-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10632-0","url":null,"abstract":"Neurodegenerative disorders, including Huntington's disease, Amyotrophic lateral sclerosis, Alzheimer's disease, and Parkinson's disease, create more challenges as the population gets older and there are no curative therapies available. Recent advances in gut microbiome research have spotlighted postbiotics and psychobiotics as innovative therapeutic strategies targeting the gut-brain axis to alleviate neurodegenerative symptoms and slow disease progression. Postbiotics, which are metabolites and cellular components released by probiotic bacteria, and psychobiotics, a class of probiotics with potential mental health benefits, offer novel approaches to neuroprotection. This chapter examines the ways in which postbiotics and psychobiotics modulate inflammation, oxidative stress, neurotrophic factors, and gut barrier integrity to provide neuroprotective effects. We review scientific research that highlights the efficacy of specific microbial strains and their metabolites in enhancing cognitive function and reducing neurodegeneration. In addition, we explore the consequences of diet and specific nutrition on strengthening the therapeutic results of these medications. The purpose of this chapter is to provide a detailed analysis of the existing data supporting the use of postbiotics and psychobiotics in both the prevention and management of neurological diseases. By integrating perspectives from microbiology, neurology, and clinical nutrition, we highlight the potential of these interventions to enhance patient outcomes and quality of life. In addition, we discuss the translational limitations and future research approaches required to successfully transition these microbiome-based treatments from the laboratory to clinical practice, emphasizing the importance of a holistic and personalized approach in combating neurodegenerative diseases.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Phage vB_LZ 2044 Deriving from K1-Type Hypervirulent Klebsiella pneumoniae Efficient Against Liver Infection Mice Model. 高效抗肝脏感染小鼠模型肺炎克雷伯菌k1型噬菌体vb_lz2044的研究

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-25 DOI: 10.1007/s12602-025-10633-z

Lingrui Shi, Yuanhui Su, Zhen Li, Li Xiang, Siyuan Liu, Xingming Li, Li Fu, Yingshun Zhou

{"title":"Characterization of a Phage vB_LZ 2044 Deriving from K1-Type Hypervirulent Klebsiella pneumoniae Efficient Against Liver Infection Mice Model.","authors":"Lingrui Shi, Yuanhui Su, Zhen Li, Li Xiang, Siyuan Liu, Xingming Li, Li Fu, Yingshun Zhou","doi":"10.1007/s12602-025-10633-z","DOIUrl":"https://doi.org/10.1007/s12602-025-10633-z","url":null,"abstract":"In order to address the issue of antimicrobial resistance posed by hypervirulent Klebsiella pneumoniae (hvKp), bacteriophages that selectively target and lyse the bacteria are being investigated as a promising therapeutic option for the treatment of clinical infections associated with hvKp. The phage vB_LZ 2044, which was isolated and characterized utilizing hvKp NTUH-K2044 as the host strain, demonstrates significant efficacy against nine distinct K1 serotype Klebsiella pneumoniae strains and exhibits an impressive lysing capacity of 396.7 PFU/cell at burst size. Moreover, it displays remarkable stability across a wide pH range (pH 4-10) and temperature range (4-40 °C), positioning it as a promising antimicrobial agent. Genetic analysis has revealed that vB_LZ 2044 is a 50419 bp double-stranded DNA phage classified under the genus Webervirus within the family Drexlerviridae. The putative Open Reading Frames (ORFs) encoded by the phage, including the perforin-endolysin-spanin system protein (ORF26, ORF27, ORF28) and the peptin lyase folds containing the tailspike protein (ORF10), are crucial for lysing the host bacteria. Furthermore, experiments conducted on mice have demonstrated the effectiveness of vB_LZ 2044 in treating hvKp-induced liver infections without inflicting additional damage to the liver tissues, thus preserving the integrity of the liver structure and mitigating inflammation. The stable biological properties and pronounced antimicrobial effects observed in the mice model suggest that phage vB_LZ 2044 holds significant potential for development as a novel antimicrobial agent.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Active Probiotic Yogurt Against Cyclophosphamide-Induced Immunosuppression in Mice by Regulating Immune Response and Gut Microbiota. 高活性益生菌酸奶通过调节免疫反应和肠道菌群对抗环磷酰胺诱导的小鼠免疫抑制。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-25 DOI: 10.1007/s12602-025-10623-1

Liyuan Yun, Jinpeng Zhang, Tiantian Zhao, Qian Li, Shuguang Fang, Xiaojuan Guo, Yanfeng Wu, YunJiao Zhao, Min Zhang

{"title":"Highly Active Probiotic Yogurt Against Cyclophosphamide-Induced Immunosuppression in Mice by Regulating Immune Response and Gut Microbiota.","authors":"Liyuan Yun, Jinpeng Zhang, Tiantian Zhao, Qian Li, Shuguang Fang, Xiaojuan Guo, Yanfeng Wu, YunJiao Zhao, Min Zhang","doi":"10.1007/s12602-025-10623-1","DOIUrl":"https://doi.org/10.1007/s12602-025-10623-1","url":null,"abstract":"Probiotics play a crucial role in modulating the immune system and maintaining intestinal epithelial barrier integrity. The study investigates the effects of high-activity probiotic yogurt on immunosuppressed mice induced by cyclophosphamide (CTX). ICR male mice (8-week-old) were injected intraperitoneally with CTX (80 mg/kg body weight/day) on days 7, 8, and 9 to establish an immunosuppressive model (n = 10). Mice were fed a normal diet or high-activity probiotic yogurt for consecutive 14 days. The effect of three high-activity probiotic yogurt(109 CFU/250 g, 1010 CFU/250 g, and 1011 CFU/250 g, named as LPY, MPY, and HPY) on immunosuppressed mice was investigated by HE staining, enzyme-linked immunosorbent assay (ELISA), Western blotting, and 16 s rRNA sequencing. Results indicated that high-activity probiotic yogurt significantly increased the immune organ indices, interleukin-6 (IL-6), interleukin-12p70 (IL-12p70), tumor necrosis factor-α (TNF-α), and improved intestinal structure (P < 0.05). Western blotting revealed that high-activity probiotic yogurt improves the expression of toll-like receptor 4 (TLR4), nuclear factor kappa-B-p65 (p65), and TNF receptor associated factor 6 (TRAF6). Furthermore, 16 s rRNA analysis demonstrated that high-activity probiotic yogurt increased the diversity and richness of the gut microbiota, and the effect of LPY performed better than MPY and HPY. These findings indicated that high-activity probiotic yogurt may improve the immune function of mice by improving intestinal homeostasis and activation of the TLR4/NF-κB signaling pathway.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Optimisation of Process Parameters for Development of Probiotic-GABA Enriched Nutri Bar by Response Surface Methodology (RSM) and Modelling Using Artificial Neural Networks (ANN): Characterisation and Sensory Evaluation by Fuzzy Logic Analysis. 修正：利用响应面法（RSM）和人工神经网络（ANN）建模优化富含gaba益生菌营养棒的工艺参数：利用模糊逻辑分析进行表征和感官评价。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-24 DOI: 10.1007/s12602-025-10610-6

Sourav Misra, Sitesh Kumar, Hari Niwas Mishra

引用次数: 0

Recent Insights About Probiotics Related Pharmabiotics in Pharmacology: Prevention and Management of Diseases. 药理学中与益生菌相关的药物研究进展：疾病的预防和管理。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-23 DOI: 10.1007/s12602-025-10613-3

Sena Davran Bulut, H Ali Döndaş, Hasan Ufuk Celebioglu, José M Sansano, Naciye Yaktubay Döndaş

引用次数: 0

Emerging Synbiotics Consisting of Catechin and Probiotic Bacteria: Exploring Aggregation, Adhesion, Antioxidant, and Anticancer Effects. 新兴的由儿茶素和益生菌组成的合成菌：探索聚集、粘附、抗氧化和抗癌作用。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-20 DOI: 10.1007/s12602-025-10631-1

Sena Davran Bulut, Buse Nur Derebası, Sevilay Günay, Yavuz Erden, Hasan Ufuk Celebioglu, Mustafa Ümit Ünal

{"title":"Emerging Synbiotics Consisting of Catechin and Probiotic Bacteria: Exploring Aggregation, Adhesion, Antioxidant, and Anticancer Effects.","authors":"Sena Davran Bulut, Buse Nur Derebası, Sevilay Günay, Yavuz Erden, Hasan Ufuk Celebioglu, Mustafa Ümit Ünal","doi":"10.1007/s12602-025-10631-1","DOIUrl":"10.1007/s12602-025-10631-1","url":null,"abstract":"Probiotic bacteria are microorganisms that confer health benefits on the host, when administered in adequate amounts. Catechin, naturally found in nature and human diet, has strong antioxidant, antibacterial, and anticancer potentials. Phenolic compounds and probiotic bacteria are found together in gastrointestinal tract (GIT), and their potential synbiotic interactions are not fully understood. The present study aims to investigate the probiotic properties effects of catechin on (bacterial growth, auto-aggregation, co-aggregation) on well-known probiotic bacteria Lactobacillus acidophilus LA-5 (LA-5) and Lacticaseibacillus rhamnosus GG (LGG), and in vitro cytotoxic effects of the synbiotic combination of catechin and probiotic bacteria on colon cancer cells Caco-2. Results show that catechin contributed to bacterial growth of LA-5, auto-aggregation of LA-5, co-aggregation of LA-5 with Staphylococcus aureus. Catechin increased the adhesion potential of LA-5 and LGG by 6% and 5%, respectively. In DPPH scavenging activity, LGG grown with catechin exerted 30% more antioxidant potential compared the LGG. Moreover, LA-5 grown in the presence of catechin led to higher cell death of Caco-2 when compared to control while it did not influence the genotoxicity property of LA-5. However, catechin did not promote the cytotoxicity and genotoxicity properties of LGG. In conclusion, catechin enhanced many physiological properties of probiotic bacteria and thus, synbiotic relationship of catechin and probiotics may offer novel insights into the prevention/treatment of colon cancer.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Characterization of Gamma-Aminobutyric Acid (GABA) from E. faecium BS5 and Its Antidiabetic Efficacy in Streptozotocin-Induced Diabetic Rats. 粪肠杆菌BS5中γ -氨基丁酸（GABA）的纯化、表征及其对链脲霉素诱导的糖尿病大鼠的降糖作用。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-19 DOI: 10.1007/s12602-025-10626-y

B S Sabna, Ramasamy Mahendran, Jeyakumar Balakrishnan, Chih-Yang Huang, P J Jerimon, Anto Thomas, Raju Eswaran, P Naveen, Angayarkanni Jayaraman

{"title":"Purification and Characterization of Gamma-Aminobutyric Acid (GABA) from E. faecium BS5 and Its Antidiabetic Efficacy in Streptozotocin-Induced Diabetic Rats.","authors":"B S Sabna, Ramasamy Mahendran, Jeyakumar Balakrishnan, Chih-Yang Huang, P J Jerimon, Anto Thomas, Raju Eswaran, P Naveen, Angayarkanni Jayaraman","doi":"10.1007/s12602-025-10626-y","DOIUrl":"https://doi.org/10.1007/s12602-025-10626-y","url":null,"abstract":"A non-proteinaceous amino acid called GABA is well-known for its physiological uses and its role as an inhibitory neurotransmitter in the brain of mammals. Apart from its neurological function, GABA has been linked to blood pressure reduction and glucose balance modulation. Since β-cell destruction and decreased insulin secretion are hallmarks of diabetes mellitus, the natural production of GABA by pancreatic β-cells increases the possibility of its therapeutic role. The aim of this study was to produce, purify, and characterize GABA from glutamic acid and assess its potential as an antidiabetic, namely to impact β-cell proliferation. GABA was produced from glutamic acid using the enzyme glutamate decarboxylase (GAD). After that, the produced GABA was purified and characterized using different analytical methods. Furthermore, in vivo studies were carried out with diabetic mice to evaluate the effect of GABA treatment on β-cell proliferation and control of insulin levels. GABA treatment improved glycaemic control in diabetic mice by increasing β-cell proliferation and regulating glucagon and insulin production. These results demonstrate the potential of GABA as an antidiabetic drug and provide a safe substitute for traditional insulin injections. Further study is needed to understand its mechanism of action and investigate its therapeutic application in the treatment of diabetes.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics Ameliorate Histological Alterations and Prevent Increases in Cytokine and Toxin Levels in Mice Infected with Various Clostridioides difficile Strains. 益生菌改善小鼠感染不同艰难梭菌菌株的组织学改变并防止细胞因子和毒素水平的增加。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-18 DOI: 10.1007/s12602-025-10586-3

Zohar Hamo, Hen Ninio, Maya Azrad, Dana Binyamin, Mor Zigdon, Halim Roshrosh, Avi Peretz

{"title":"Probiotics Ameliorate Histological Alterations and Prevent Increases in Cytokine and Toxin Levels in Mice Infected with Various Clostridioides difficile Strains.","authors":"Zohar Hamo, Hen Ninio, Maya Azrad, Dana Binyamin, Mor Zigdon, Halim Roshrosh, Avi Peretz","doi":"10.1007/s12602-025-10586-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10586-3","url":null,"abstract":"The severity of C. difficile infection (CDI) varies and can be influenced by bacterial strain, toxin levels, and antibiotic treatment. Current treatments are limited due to high recurrence rates and potential risks. This study aimed to characterize the manifestations of CDIs induced by various bacterial strains in mice and to assess the effects of different probiotic strains on these manifestations, with a focus on intestinal alterations, immune response, and toxin concentrations. The tested C. difficile strains induced significant intestinal damage and disrupted intestinal epithelial integrity, as indicated by reduced ZO-1 levels, with responses varying across strains. In addition, IL-6, TNF-α, and secreted toxin levels varied across the animal groups, with the highest levels seen in mice infected with sequence type 42 or 104. Administration of Lactobacillus acidophilus, Bifidobacterium bifidum, or Lactobacillus paracasei following CDI induction reduced intestinal damage, with the degree of rescue varying between probiotic strains. Lactobacillus paracasei demonstrated the most notable potential in alleviating CDI, marking its potential as an adjunct to conventional therapies for CDI.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0