Probiotics and Antimicrobial Proteins最新文献

{"title":"Clostridium dalinum: A Novel Lactic Acid Bacterium with Excellent Antioxidant and Anti-inflammatory Functions Through NF-κB, Keap1-Nrf2-ARE Signaling Pathways.","authors":"Zheng-Qiang Li, Rui Li, Wei Deng, Rui An, Ping Ma, Jun Li, Rong She, Xiao-Yan Yang, Xu Yang, Wen Xiao","doi":"10.1007/s12602-025-10655-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10655-7","url":null,"abstract":"<p><p>The health benefits of probiotics are widely acknowledged, and the development of probiotics with antioxidant and anti-inflammatory properties is expected to further advance the probiotic industry and enhance human health. This study reports a novel lactic acid strain, Clostridium dalinum, isolated from soil. As a member of the Clostridium genus, this strain demonstrates strong coagulation properties and a high safety profile. Notably, C. dalinum exhibits exceptional gastrointestinal tolerance, retaining viability under acidic conditions (pH 3.0 for 2 h) and high bile stress (3% bile for 3 h). Evaluation using Caco-2 cells confirmed that both the strain and the yogurt fermented with it exhibit antioxidant and anti-inflammatory functions mediated through the NF-κB and Keap1-Nrf2-ARE signaling pathways. Metabolomics analysis identified significantly elevated levels of antioxidant and anti-inflammatory metabolites including Chavicol, Neocnidilide, Procaine, and 6-Phosphogluconic acid in the C. dalinum group, functionally linked to amino acid, lipid, and cofactor metabolism pathways. Pathway enrichment analysis further demonstrated specific activation of butanoate metabolism by C. dalinum. In the D-yogurt group, key metabolites were similarly upregulated, while purine metabolism was uniquely downregulated. These findings confirm that C. dalinum is a safe probiotic with notable antioxidant and anti-inflammatory properties, demonstrating potential for immune modulation and therapeutic applications in intestinal damage and inflammation. Importantly, this discovery offers a novel perspective for probiotic development.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotics: Modulation of the Gut Microbiota and Potential for Association with Nanotechnology.","authors":"Ana Júlia Rocha Cardoso, Suzana Gonçalves Carvalho, Vitória Ribeiro Mantovanelli, Leonardo de Freitas Marinho, Amanda Letícia Polli Silvestre, Tânia Forster-Carneiro, Maria Palmira Daflon Gremião, Marlus Chorilli","doi":"10.1007/s12602-025-10675-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10675-3","url":null,"abstract":"<p><p>Postbiotics are preparations containing inanimate microorganisms or their components that have a direct or indirect beneficial effect on the host and can include many different components, such as short-chain fatty acids, bacterial lysates, cell-free culture supernatants, cell wall fragments, enzymes, and exopolysaccharides. Despite the numerous advantages of postbiotics for the body, their use is limited due to unfavorable pharmacokinetics, low bioavailability, non-selective pharmacological action, poor palatability, and unpleasant odor. The development of nanotechnology-based delivery systems could be an important industrial tool to enhance the selective action of these agents and improve their therapeutic efficacy, particularly in the pharmaceutical and food sectors. In the pharmaceutical industry, several nanotechnology-based delivery systems have already been approved by the FDA and successfully marketed, demonstrating their clinical and industrial viability, such as Abraxane®, Doxil® (or Caelyx®), Onivyde®, Marqibo®, and Vyxeos®. In the food industry, products such as Driphorm® (nanostructured lipid emulsion for the efficient delivery of vitamin D in food) illustrate how nanotechnology has been applied in concrete ways to the development of functional foods and nutraceuticals, contributing to technological innovation, added value, and advances in health promotion. However, although nanotechnology in the delivery of postbiotics is an emerging and innovative field with promising preclinical studies, the lack of consolidated clinical or commercial data means that this topic needs to be expanded in order to achieve standardization and clinical validation. This review aims to highlight the importance of postbiotics in the modulation of gut microbiota and in the prevention/treatment of disease, as well as the potential of using nanotechnology to facilitate the targeting of these compounds and the limitations of using these systems for such applications.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum GUANKE Alleviates Intestinal Barrier Damage Caused by Influenza Infection in Mice.","authors":"Kun Yue, Simin Lu, Tao Yang, Jielan Mi, Siqin He, Hanyu Ma, Yujia He, Zhihan Yang, Yuanming Huang, Liqiong Song, Zhihong Ren, Lili Ren, Jianguo Xu","doi":"10.1007/s12602-025-10665-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10665-5","url":null,"abstract":"<p><p>Influenza, a highly transmissible acute respiratory illness caused by influenza viruses, is responsible for annual seasonal epidemics and poses a substantial global disease burden. While primarily recognized for its respiratory manifestations, influenza infection exhibits significant extrapulmonary effects, particularly through disrupting gut microbiota composition and compromising intestinal barrier integrity. This study investigates the protective efficacy of Lactiplantibacillus plantarum strain GUANKE against influenza-associated intestinal pathology in a murine model. Building on GUANKE's established safety profile and immunomodulatory capabilities, we employed a multi-faceted approach examining three key defense mechanisms: (1) maintenance of intestinal epithelial integrity, (2) preservation of chemical mucosal secretions, and (3) modulation of microbial homeostasis. Our findings establish crucial scientific evidence for probiotic-mediated protection of gastrointestinal function during influenza infection, potentially informing novel therapeutic strategies for virus-induced gut dysbiosis.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144643247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating Clostridium perfringens-Induced Gut Lesionsin Broiler Chickens by Orally Administered Bovine Intestinal Alkaline Phosphatase.","authors":"Wangli Ji, Daoxin Yang, Wenjing Zhang, Yuan Wang, Zhenzhen Hao, Kairui Guo, Xianhua Sun, Shuai Wang, Shuyan Yang, Jianshuang Ma, Huiying Luo, Bin Yao, Meiling Zhang, Honglian Zhang, Huoqing Huang, Xiaoyun Su","doi":"10.1007/s12602-025-10664-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10664-6","url":null,"abstract":"<p><p>Intestinal alkaline phosphatase (AP) detoxifies lipopolysaccharides, which has been exploited in treating infection of Gram-negative bacterial pathogens such as Escherichia coli. Orally administered AP has the potential to modulate the gut microbiota, which is in part ascribed to its ability to degrade ATP, a well-known inhibitor of gut commensal bacteria. In addition, AP can fortify the gut barrier. Therefore, we hypothesized that the enzyme might also be used to control intestinal Gram-positive pathogens such as Clostridium perfringens. Herein, broiler chickens were challenged with 3 × 10⁸ colony-forming units of C. perfringens daily from 14 to 20 days. Low (1000 U/kg of feed) and high (5000 U/kg of feed) doses of a recombinant bovine intestinal AP (bIAP) were orally administered to the chickens throughout the study. bIAP could indeedalleviate the gut lesion and diarrhea symptom in chickens challenged with C. perfringens and reversed the decline in their growth performance. 16S rRNA gene sequencing and non-targeted metabolomics analyses revealed that bIAP could modulate the gut microbiota, which was accompanied with a change of the gut metabolites profile, the improved intestinal integrity and immunity, and an ultimate protection of the animals from C. perfingens infection. Strikingly, the RT-qPCR assay showed that the transcript levels of key tight junction proteins zonula occludens-1, Mucin 2, claudin-1, and occludin in the duodenum, jejunum, and ileum were even superior in the high dose group than those in the unchallenged group, suggestive of enhanced integrity of the intestinal barrier. Enzymes such as bIAP are, therefore, a powerful tool in modulating the gut microbiota for better health of the host animals.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144643246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriocin-Mediated Silver Nanoconjugate: Synthesis, Characterization, and Application as an Antibiofilm Agent Against Two Common Pathogenic Bacteria.","authors":"Bandita Dutta, Ujjal Das, Sedevino Ltu, Sreejita Ghosh, Rina Rani Ray","doi":"10.1007/s12602-025-10656-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10656-6","url":null,"abstract":"<p><p>Antibiotic resistance is generated due to rampant misuse of antibiotics, and almost 80% of persistent and hard-to-treat disease-causing bacteria are difficult to treat with antibiotics because of the presence of biofilm. Hence, a different approach must be implemented in such a way that these biofilm-forming recalcitrant infectious agents can be treated without causing antibiotic resistance to develop. Bacteriocins from lactic acid bacteria, although they offer a safer therapeutic option but are limited by their narrow antimicrobial scope and the need for high doses. To address these constraints, this study focused on synthesizing bacteriocin-capped nanoparticles and evaluating their antibiofilm activities. A purified bacteriocin extracted from a strain of Enterococcus faecalis BDR22 was used for biogenic synthesis of silver nanoparticles (AgNPs). The efficacies of the biogenic nanoparticle (Bac-AgNP) against two nosocomial disease-causing bacteria, Pseudomonas aeruginosa ATCC 10145 and Staphylococcus aureus ATCC 23235, were evaluated, which indicated a significant reduction in bacterial biofilm 81.82667 ± 0.03163% (S. aureus) and 78.43 ± 0.03796% (P. aeruginosa) at a concentration of 1.7 μg/mL. It has the ability to disrupt the EPS matrix within the bacterial biofilm, which was further confirmed through SEM and FTIR analyses. The flow cytometric analysis clearly depicts the intracellular ROS generation and death of bacterial cells after treatment with Bac-AgNP. This synthesized nanoparticle was found as non-cytotoxic against the HaCat cell line, approving the candidacy of these particles as an effective therapeutic strategy to treat nosocomial infections.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation with Recombinant Bacillus subtilis Expressing Insulin-Like Growth Factor 1a (IGF1a) Promotes Muscle Hypertrophy in Zebrafish (Danio rerio).","authors":"Jade Riet, Bruna Félix Nornberg, João Costa-Filho, Raiza Dos Santos Azevedo, Arthur Cardoso, Laura Dall'Agno, Ana Miguel Magalhães, Iuri Salim Abou Anni, Marcela Meirelles, Mirna Enríquez Reyes, Arthur Zanetti Nunes Fernandes, Tony Silveira, Izani Bonnel Acosta, Antonio Sergio Varela Junior, Vinicius Farias Campos, Luis Fernando Marins","doi":"10.1007/s12602-025-10658-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10658-4","url":null,"abstract":"<p><p>Aquaculture is a key sector for animal protein production that has shown significant growth, which can be further improved in terms of carcass yield and fillet quality. Enhancing skeletal muscle growth in fish through biotechnological strategies is a promising alternative with substantial commercial application potential. Fish growth regulation occurs via the somatotropic axis, where Insulin-Like Growth Factor 1 (IGF1) plays a critical role in skeletal muscle hyperplasia and hypertrophy. In this study, a strain of the probiotic bacterium Bacillus subtilis was genetically modified to express zebrafish (Danio rerio) IGF1a, which was used as a dietary supplement for zebrafish. The experiment lasted 60 days, during which zootechnical performance, muscle morphometry, and the gene expression of the IGF system, as well as muscle development genes, were evaluated. The results demonstrated that supplementation with the genetically modified probiotic improved zootechnical performance and induced muscle hypertrophy in the fish. Additionally, it promoted the induction of IGF1Rb receptor and Myf5 expression in skeletal muscle. These findings suggest that IGF1a produced and secreted by B. subtilis in the zebrafish intestine was able to trigger an endocrine action, interacting with the IGF1Rb receptor in the muscle and promoting hypertrophy. The results provide evidence of the potential of B. subtilis as a biofactory for growth factors capable of impacting the host and inducing tissue-specific growth, which could enhance carcass yield in fish used in commercial aquaculture.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Postbiotics in the Biodegradation of Antinutrients in Foods.","authors":"Nader Khani, Amir Hessam Shakeri, Mansour Rabie Ashkezary, Baharak Aghapour, Roya Abedi Soleimani, Negin Hosseinzadeh, Negin Rezaei-Savadkouhid, Ramin Khorrami, Seyed Mohamad Javad Shkouhian, Aziz Homayouni-Rad","doi":"10.1007/s12602-025-10649-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10649-5","url":null,"abstract":"<p><p>Antinutrients, frequently called as anti-nutritional factors (ANFs), comprise a class of compounds prevalent in numerous plant-derived foodstuffs that can impede the bioavailability of essential nutrients or serve as precursors to harmful substances. The impact of ANFs on human health remains a topic of contention, primarily contingent upon their concentrations. While the beneficial ramifications of these compounds are extensively documented, the associated risks they present and the methodologies to mitigate such risks have not received equivalent scholarly attention. It is unequivocal that various ANFs detrimentally influence the absorption of vitamins, minerals, and proteins, inhibiting certain enzymatic activities, thereby adversely affecting the bioavailability of nutrients within the human organism. The principal aim of this investigation is to examine the potential for modulating the levels of ANF in food products via fermentation. This review focuses on possibly controlling ANF levels in food through biological methods. The use of probiotics and their metabolites (postbiotics) is a novel approach to ANF control. Postbiotics are defined as soluble entities (either direct products or metabolic byproducts) that are secreted by living bacteria or released after bacterial lysis; these include enzymes, peptides, teichoic acids, muropeptides derived from peptidoglycan, polysaccharides, cell surface proteins, and organic acids. The interest in these postbiotics is attributable to their well-defined chemical structures, established safety dose thresholds, prolonged shelf lives, and the presence of diverse signaling molecules that may exhibit nutraceutical properties (such as cholesterol assimilation, the degradation of undesirable or anti-nutritional compounds, and the production or enhanced bioavailability of antioxidants). This review highlights the role of postbiotics in the degradation of ANFs.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Hydrogel Formulation Combining Phycocyanin and Probiotic for Enhancing Skin Regeneration and Accelerated Wound Healing: A Preclinical Investigation in Wistar Rats.","authors":"Asmaa Negm El-Dein, Tarek N Soliman, Asmaa Ezzat, Marwa Adel Abd El-Fattah, Hanan Farouk Aly, Eman A Younis, Nasser S Flefil","doi":"10.1007/s12602-025-10635-x","DOIUrl":"https://doi.org/10.1007/s12602-025-10635-x","url":null,"abstract":"<p><p>Skin repair is a global concern that requires multifunctional wound healing platforms that accelerate healing and shield the wound from external contamination. Phycocyanin (C-PC) is a natural protein that shows favorable characteristics for skin repair. The present study aims to investigate the synergistic wound healing potential of probiotic Pediococcus pentosaceus and C-PC hydrogel formulation. C-PC whey protein nanofibrils (WPINF) were first prepared and evaluated for particle size, % entrapment efficiency, and zeta potential. Selected WPINF was used to prepare several hydrogel formulations which were further evaluated for physical properties and in vitro C-PC release. Formulation with acceptable physical properties and C-PC release was studied for oxidative stress markers, antioxidant biomarkers, and wound healing effect in animal models. WPINF had acceptable particle size (26.59-153.5 nm), % EE (65.76-95.98%), and zeta potential (22.3-26.1 mV). The developed hydrogels exhibited neutral pH values (6.97-7.10), optimal spreadability (5.60-7.50 cm), and viscosity (123-209 Pa). HEC-based hydrogel (F1) showed superior C-PC release which was found to follow Higuchi diffusion. In vivo, hydrogel formulations (with and without probiotic) showed significantly reduced oxidative stress markers, with MDA reduced by 54.76-98.58% and antioxidant biomarkers (GSH and TAC) ensuring successful delivery of therapeutic compounds. Histological analysis showed improved re-epithelialization, reduced inflammation, and well-formed granulation tissue in treated groups, with probiotic-C-PC group demonstrating additional immunomodulatory effects. The study highlights promising potential of C-PC-probiotic hydrogels as a safe and effective biotherapeutics for wound healing, supporting their advancement into further clinical investigations to validate their efficacy in humans.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Functional Characterization of an Ancestral Hepcidin-Like Antimicrobial Peptide in the Lamprey (Lethenteron camtschaticum).","authors":"Jiayi Qiao, Xiaxia Wang, Chaoyue Zhou, Chennan Li, Yue Pang, Qingwei Li, Yimu Luan, Meng Gou","doi":"10.1007/s12602-025-10654-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10654-8","url":null,"abstract":"<p><p>Hepcidin, also known as LEAP-1 (liver-expressed antimicrobial peptide), is a cysteine-rich, cationic antimicrobial peptide found in vertebrates that plays a key role in iron transport and immune response. Although hepcidin has been characterized in various vertebrates, including fish and mammals, its evolutionary origin remains unclear. In this study, the ancestral hepcidin gene (named Lc-HAMP) was cloned and characterized from the liver of the primitive jawless vertebrate Lethenteron camtschaticum (lamprey). The gene encodes a 25-amino-acids signal peptide and a mature 23-amino- acids peptide. Despite relatively low sequence similarity with other species, the mature Lc-HAMP peptide retains eight conserved cysteine residues that form a core structure of four disulfide bonds. Lc-HAMP expression is significantly upregulated upon multiple immune challenges, and the mature peptide exhibits dose-dependent in vitro antimicrobial activity against a wide range of bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, and Aeromonas, but shows no activity against Escherichia coli and Pseudomonas aeruginosa. Lc-HAMP induces bacterial membrane damage and triggers reactive oxygen species (ROS) bursts in bacteria. Transcriptomic and metabolomic analyses indicate that overexpression of Lc-HAMP in HEK293T cells affects endoplasmic reticulum stress and glutathione metabolism, similarly to observations in higher vertebrates. These findings shed light on the evolutionary origin of hepcidin, a key antimicrobial peptide, and suggest potential strategies for preventing and controlling immunosuppression in lower vertebrates such as fish.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Adipogenic Effects of Probiotic Lactiplantibacillus pentosus THF59 via Inhibition of Differentiation and Promotion of Apoptosis in 3T3-L1 Cells.","authors":"Qiwen Zheng, Trang Thi Minh Nguyen, Xiangji Jin, Gyeong-Seon Yi, Tae-Hoo Yi","doi":"10.1007/s12602-025-10663-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10663-7","url":null,"abstract":"<p><p>This study investigated the probiotic properties and anti-adipogenic activities of Lactiplantibacillus pentosus THF59, isolated from blueberries. THF59 exhibited several probiotic traits, including antimicrobial activity, tolerance to simulated gastric juice and bile salts, non-pathogenicity, and strong adhesion to intestinal epithelial Caco-2 cells. To evaluate its anti-adipogenic potential, MDI-induced 3T3-L1 preadipocytes were treated with THF59 cell-free supernatant (CFS). At the highest tested concentration (100 μg/mL), THF59 CFS significantly reduced intracellular lipid accumulation by 68.40%, as evidenced by Oil Red O staining. It also decreased intracellular reactive oxygen species (ROS) levels by 27.45%, potentially suppressing adipogenic differentiation by disrupting ROS-mediated early signaling events. Moreover, THF59 alleviated MDI-induced S-phase cell cycle arrest, suggesting interference with early-stage preadipocyte differentiation. At this concentration, THF59 CFS significantly downregulated key adipogenic transcription factors involved in various stages of differentiation, including SREBP-1 (68.91%), PPARγ (50.75%), C/EBPα (41.99%), and AP-2 (37.44%). These findings indicate that THF59 suppressed adipogenesis through multi-level regulatory interference, from early commitment to terminal differentiation. Notably, THF59 also promoted late-stage apoptosis in mature adipocytes by 6.55%, representing an additional lipid-lowering mechanism through the removal of lipid-storing cells. In conclusion, THF59 exerted anti-adipogenic effects in MDI-induced 3T3-L1 preadipocytes by inhibiting differentiation and promoting apoptosis, supporting its potential as a functional probiotic candidate for obesity prevention.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}