Probiotics and Antimicrobial Proteins最新文献

{"title":"A Study of Bioactivities and Composition of a Cocktail of Supernatants Derived from Lactic Acid Bacteria for Potential Food Applications.","authors":"Xiaohui Huang, Franks Kamgang Nzekoue, Junbiao Wang, Anna Rita Attili, Maria Magdalena Coman, Maria Cristina Verdenelli, Dennis Fiorini, Giacomo Rossi, Cristina Marchini, Cristina Miceli, Stefania Silvi","doi":"10.1007/s12602-024-10442-w","DOIUrl":"https://doi.org/10.1007/s12602-024-10442-w","url":null,"abstract":"<p><p>Growing interests in replacing conventional preservatives and antibiotics in food and pharmaceutical industries have driven the exploration of bacterial metabolites, especially those from strains with generally recognized as safe (GRAS) status, such as lactic acid bacteria (LAB). In this study, a supernatant cocktail derived from multiple LAB strains was prepared and its bioactivities-antimicrobial, antibiofilm, antioxidant, cytotoxicity, and stability-were thoroughly investigated. The cocktail's main components were identified using thermal and protease treatments, gas chromatography coupled to mass spectrometry (GC-MS), and flame ionization detection (GC-FID). The results demonstrated that the supernatant cocktail had a broad inhibition spectrum and was effective against food-related bacterial indicators with the highest activity observed on Bacillus cereus ATCC9634 (inhibition zone sizes 12.33 mm) and the lowest on Enterococcus faecium DSM 13590 (3.31 mm). It showed dose- and time-dependent delaying effects on the growth of tested fungi. Regarding the antibiofilm activity, it was more effective in preventing biofilm formation (40% biofilm mass reduction) than in degrading preformed biofilm (20% reduction). Additionally, the cocktail showed antioxidant capacity of 10.1 ± 0.3 g Trolox equivalent (TE)/kg and dose-dependent cytotoxicity on HEK-293 and HT-29 cell lines. The main bioactive compounds in this cocktail are organic acids (particularly acetic acid), volatiles, and bacteriocin-like compounds. The antimicrobial capacity of this supernatant cocktail was highly reproducible across different fermentation batches, and it remained highly stable at 4 °C. Overall, these findings provided novel insights into the functional potentials of LAB metabolites, broadening their application as customizable biopreservatives for food and pharmaceutical industry.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Peptides Against Arboviruses: Mechanisms, Challenges, and Future Directions.","authors":"Iman Owliaee, Mehran Khaledian, Ali Shojaeian, Hamid Madanchi, Reza Yarani, Armin Khaghani Boroujeni, Mohammad Shoushtari","doi":"10.1007/s12602-024-10430-0","DOIUrl":"https://doi.org/10.1007/s12602-024-10430-0","url":null,"abstract":"<p><p>This review delves into the potential of antimicrobial peptides (AMPs) as promising candidates for combating arboviruses, focusing on their mechanisms of antiviral activity, challenges, and future directions. AMPs have shown promise in preventing arbovirus attachment to host cells, inducing interferon production, and targeting multiple viral stages, illustrating their multifaceted impact on arbovirus infections. Structural elucidation of AMP-viral complexes is explored to deepen the understanding of molecular determinants governing viral neutralization, paving the way for structure-guided design. Furthermore, this review highlights the potential of AMP-based combination therapies to create synergistic effects that enhance overall treatment outcomes while minimizing the likelihood of resistance development. Challenges such as susceptibility to proteases, toxicity, and scalable production are discussed alongside strategies to address these limitations. Additionally, the expanding applications of AMPs as vaccine adjuvants and antiviral delivery systems are emphasized, underscoring their versatility beyond direct antiviral functions.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Supplements of Combined Lactobacillus plantarum and Asparagus officinalis Modulate Gut Microbiota and Alleviate High-Fat Diet-Induced Cognitive Deficits and Neurodegeneration in Rats.","authors":"Nancy N Shahin, Omar A Ahmed-Farid, Ebtehag A E Sakr, Enas A Kamel, Maha M Mohamed","doi":"10.1007/s12602-024-10429-7","DOIUrl":"https://doi.org/10.1007/s12602-024-10429-7","url":null,"abstract":"<p><p>High-fat diet (HFD) consumption disrupts the gut microbiome, instigating metabolic disturbance, brain pathology, and cognitive decline via the gut-brain axis. Probiotic and prebiotic supplementation have been found to improve gut microbiome health, suggesting they could be effective in managing neurodegenerative disorders. This study explored the potential benefits of the probiotic strain Lactobacillus plantarum 20174 (L. plantarum), prebiotic Asparagus officinalis (A. officinalis) extract, or their synbiotic combination against HFD-induced cognitive dysfunction and neurodegeneration in rats. Male Sprague-Dawley rats were fed either a normal diet or an HFD for 24 weeks. Starting from week 13, rats on either diet were divided into vehicle-, prebiotic-, probiotic-, and synbiotic-treated subgroups. Rats received their assigned intervention for 12 more weeks. Prebiotic, probiotic, or synbiotic treatment reverted HFD-instigated alterations in hippocampal amyloid beta, p-tau, α-synuclein, and BDNF levels, leading to restored cognitive function. The tested therapies also improved the HFD-disrupted lipid profile. Interestingly, probiotic and synbiotic therapies attenuated oxidative stress and inflammation, reinstated neurotransmitter balance, and mitigated the energy deficit in HFD-fed rats. Furthermore, L. plantarum and Asparagus administration modulated gut microbiota composition by raising Lactobacillus species and reducing Coliform and Staphylococci bacteria as well as fungi populations. These findings suggest that the oral consumption of A. officinalis prebiotics and/or L. plantarum probiotics alleviates HFD-induced cognitive deficit and neurodegeneration through modulation of the gut-brain axis with superior restorative effects being achieved by synbiotic treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-Bioprinted Urinary Catheters Enable Sustained Probiotic Recovery Under Flow and Improve Bladder Colonization In Vivo.","authors":"Anthony J Kyser, Arielle Greiner, Victoria Harris, Rudra Patel, Hermann B Frieboes, Nicole M Gilbert","doi":"10.1007/s12602-024-10428-8","DOIUrl":"https://doi.org/10.1007/s12602-024-10428-8","url":null,"abstract":"<p><p>Catheter-associated urinary tract infections (CAUTIs) account for a large proportion of healthcare-associated infections. CAUTIs, caused by colonization of the catheter surface by uropathogens, are challenging to treat, especially when compounded by antibiotic resistance. One prophylactic strategy that could reduce pathogen colonization is bacterial interference, whereby the catheter surface is coated with non-pathogenic bacteria. Current challenges include identifying appropriate bacterial interference strains that maintain stable association with the catheter and are viable, but not pathogenic, in the urinary tract environment. This study evaluated the stability of probiotic Lactobacillus rhamnosus in 3D bioprints mimicking urinary catheter tubing under urine flow and assessed viability and safety in an in vivo mouse model. Bioprints underwent hydraulic flow testing in vitro with artificial urine media (AUM), followed by evaluation of catheter structure, L. rhamnosus recovery, and biofilm formation. Mice were inoculated with free L. rhamnosus bacteria or implanted with L. rhamnosus-containing bioprints to measure urinary tract colonization and assess effects on the bladder tissue. Bioprinted segments exhibited minimal mass change while maintaining an intact shape and demonstrated viable L. rhamnosus recovery throughout 7 days. L. rhamnosus formed biofilms on the bioprint surface that were not disrupted by urinary flow conditions. Encouragingly, L. rhamnosus viability was maintained in bioprints in a mouse urinary tract catheterization model. Bioprints released L. rhamnosus in vivo and did not cause histological inflammation beyond that generated by standard silicone catheters. In summary, L. rhamnosus bioprints exhibited key desirable characteristics, including maintenance of probiotic viability, probiotic growth on the catheter surface, and enhanced probiotic colonization of the bladder. This study supports the development of bioprinted probiotic catheters as a new strategy to prevent CAUTI.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, Delivery, and Regulatory Aspects for Application of Plant-Based Anti-microbial Peptides: a Comprehensive Review.","authors":"Praveen Nagella, Balamuralikrishnan Balasubramanian, Sungkwon Park, Udisha Singh, Arpita Jayan, Saptadeepa Mukherjee, Aatika Nizam, Arun Meyyazhagan, Manikantan Pappuswamy, Joseph Kadanthottu Sebastian, Vasantha Veerappa Lakshmaiah, Amin Mousavi Khaneghah","doi":"10.1007/s12602-024-10421-1","DOIUrl":"https://doi.org/10.1007/s12602-024-10421-1","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Antimicrobial peptides (AMPs) are small, positively charged biomolecules produced by various organisms such as animals, microbes, and plants. These AMPs play a significant role in defense mechanisms and protect from adverse conditions. The emerging problem of drug resistance in microbes poses a global health challenge in treating diseases. This plant-based antimicrobial peptide is a promising candidate for fighting against drug-resistant microbes. The PAMPs process specific key properties, proving their efficacy as antimicrobial agents against a broad spectrum of microbes such as Gram-positive, Gram-negative, and fungi. Extensive research on PAMPs has explored their potential as plant growth regulators and therapeutic agents. Their diverse mode of action on microbes encouraged their application in food industries. The PAMPs are isolated and purified from various plant species' organs such as roots, shoots, leaves, flowers, and seeds. These are bioactive molecules with significant stability, and low toxicity has encouraged their application as food additives. Furthermore, to meet the consumer demand, mass production of AMPs was possible with recombinant DNA technology. The advanced and nanotechnology-based delivery system has significantly improved the efficacy and bioavailability of PAMPs as food preservatives for improved shelf-life and prevent spoilage of food products. The PAMPs are of green origin and can be used as natural bio preservatives that do not alter the sensory properties of food and are harmless to consumers. Plants being the rich resource of AMPs to support their quick identification, and retrieval for commercial applications there is a need to integrate the omics approach with databases. The AMPs are small, positively charged biomolecules produced by various organisms such as animals, microbes, and plants. These AMPs play a significant role in defense mechanisms and protect from adverse conditions. The emerging problem of drug resistance in microbes poses a global health challenge in treating diseases. This plant-based antimicrobial peptide is a promising candidate for fighting against drug-resistant microbes. The PAMPs process specific key properties, proving their efficacy as antimicrobial agents against a broad spectrum of microbes such as Gram-positive, Gram-negative, and fungi. Extensive research on PAMPs has explored their potential as plant growth regulators and therapeutic agents. Their diverse mode of action on microbes encouraged their application in food industries. The PAMPs are isolated and purified from various plant species' organs such as roots, shoots, leaves, flowers, and seeds. These are bioactive molecules with significant stability, and low toxicity has encouraged their application as food additives. Furthermore, to meet the consumer demand, mass production of AMPs was possible with recombinant DNA technology. The advanced and nanotechnology-based delivery system has significantly improved the efficacy ","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics Exert Gut Immunomodulatory Effects by Regulating the Expression of Host miRNAs.","authors":"Wenjing Li, Yongwei Zeng, Jiayu Zhong, Youyu Hu, Xia Xiong, Yingshun Zhou, Li Fu","doi":"10.1007/s12602-024-10443-9","DOIUrl":"https://doi.org/10.1007/s12602-024-10443-9","url":null,"abstract":"<p><p>Probiotics exert a diverse range of immunomodulatory effects on the human gut immune system. These mechanisms encompass strengthening the intestinal mucosal barrier, inhibiting pathogen adhesion and colonization, stimulating immune modulation, and fostering the production of beneficial substances. As a result, probiotics hold significant potential in the prevention and treatment of various conditions, including inflammatory bowel disease and colorectal cancer. A pivotal mechanism by which probiotics achieve these effects is through modulating the expression of host miRNAs. miRNAs, non-coding RNA molecules, are vital regulators of fundamental biological processes like cell growth, differentiation, and apoptosis. By interacting with mRNAs, miRNAs can either promote their degradation or repress their translation, thereby regulating gene expression post-transcriptionally and modulating the immune system. This review provides a comprehensive overview of how probiotics modulate gut immune responses by altering miRNA expression levels, both upregulating and downregulating specific miRNAs. It further delves into how this modulation impacts the host's resistance to pathogens and susceptibility to diseases, offering a theoretical foundation and practical insights for the clinical utilization of probiotics in disease prevention and therapy.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum P101 Alleviates Liver Toxicity of Combined Microplastics and Di-(2-Ethylhexyl) Phthalate via Regulating Gut Microbiota.","authors":"Jiajun Guo, Tao You, Xiaoyan Feng, Hengyi Xu","doi":"10.1007/s12602-024-10439-5","DOIUrl":"https://doi.org/10.1007/s12602-024-10439-5","url":null,"abstract":"<p><p>Microplastics (MPs) and Di-(2-ethylhexyl) phthalate (DEHP) as emerging contaminants, have caused increasing concern due to their co-exposure risks and toxicities to humans. Lactic acid bacteria have been demonstrated to play a significant role in the mitigation of organismal damage. Probiotic intervention is widely recognized as a safe and healthy therapeutic strategy for targeting the mitigation of organic damage. This study explored the effectiveness and underlining mechanism of an excellent probiotic property Lactiplantibacillus plantarum P101 (L. plantarum P101) to the combined hepatotoxicity of MPs and DEHP. In this study, mice were exposed to DEHP and MPs via free drinking water, followed by intervention with L. plantarum P101. Results showed that co-exposure to DEHP and MPs caused severe oxidative stress and inflammation in the liver and intestines, which was reversed after probiotic intervention. Moreover, the intervention reshaped the structure of gut microbiota and alleviated the liver damage after the combined exposure. Together, we found the intervention of L. plantarum P101 effectively mitigated the toxic effects on the liver system caused by the co-exposure to MPs and DEHP, offering a promising strategy for reducing the combined toxicity of these substances.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Bacteriological Quality and Antibiotic Resistance in Locally and Industrially Produced Ice Creams: A Study on Probiotic Incorporation for Enhanced Safety and Health Benefits in Kushtia City, Bangladesh.","authors":"Muntaha Binte Mukhles, Abdullah Al Mamun, Md Khasrul Alam, Md Amdadul Huq, Md Abul Kalam Azad, Md Rasul Karim, M Mizanur Rahman","doi":"10.1007/s12602-024-10446-6","DOIUrl":"https://doi.org/10.1007/s12602-024-10446-6","url":null,"abstract":"<p><p>Ice creams are consumable foods that have the potential to be used as probiotic carriers. The purpose of this study was to evaluate the bacteriological quality (i.e., antibiotic response, bacterial identification, and fermentation using bacterial culture) of ice creams. The 16S rDNA sequence analysis was used to identify the isolates, and the disc diffusion method was used to measure antibiotic responses. The total viable counts (TVC) of all the branded ice cream samples were within the permitted limits (1.0 × 10⁵ cfu/ml) stipulated by Bangladesh guidelines. On the other hand, locally produced ice cream was not within permitted limits, exceeding these criteria and suggesting possible health hazards. Six (6) antibiotic-resistant bacteria were identified by 16S rDNA sequencing, namely Brevundimonas sp., Exiguobacterium sp., Brevundimonas terrae-IARI-ABR-33., Pseudomonas geniculate, Stenotrophomonas sp., and Brevundimonas terrae. Lactobacillus acidophillus had antagonistic activity against test pathogens, exhibiting varied degrees of inhibition. To make probiotic ice cream, a standard ice cream mix was fermented using L. acidophillus bacteria. After the fermentation mixture was frozen for storage, there was a one log cycle decline in Lactobacillus viable counts. So, it may be concluded that probiotic ice cream created with L. acidophillus will be safer, healthier, and more hygienic than industrial or locally produced ice cream.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saccharomyces boulardii Ameliorates LPS-Induced Amyloidogenesis in Rats.","authors":"Ghazaleh Mohammadi, Fatemeh Babaei, Faezeh Golpour, Fatemeh Sadat Rashidi, Shiva Ghafghazi, Leila Dargahi, Marjan Nassiri-Asl","doi":"10.1007/s12602-024-10445-7","DOIUrl":"https://doi.org/10.1007/s12602-024-10445-7","url":null,"abstract":"<p><p>Gut brain axis can affect the incidence of Alzheimer's disease (AD). Probiotics restore the homeostasis of gut dysbiosis and prevent AD. Here, we evaluated the impact of Saccharomyces boulardii on rats with lipopolysaccharide (LPS)-induced amyloidogenesis. Rats were classified into four groups: (1) Control (saline), (2) LPS 250 µg/kg (saline + LPS), (3) S. boulardii (10¹⁰ CFU/mL/rat), and (4) S. boulardii (10¹⁰ CFU/mL/rat) + LPS (250 μg/kg). The passive behavioral test, Western blotting, and immunohistochemistry were done using the animal hippocampi. Step-through latency (STL) indicated that the LPS-treated group had decreased memory retrieval compared to the control group. The LPS group had increased hippocampal levels of amyloid-β peptide, amyloid-β precursor protein (APP), and β-secretase (BACE). Administration of the S. boulardii before LPS prolonged STL which has been shortened in the LPS group (P < 0.05). In the LPS + S group, S. boulardii reduced the levels of APP significantly compared to the LPS group (P < 0.01). S. boulardii mitigated Aβ buildup and memory dysfunction caused by LPS through modulating the APP, BACE1, and Aβ pathways. Future studies are required to explain the neuroprotective effects of S. boulardii, since it could be a novel therapy or prevention strategy for AD.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Characterization, Biological Activities, and Potential Applications of the Antimicrobial Peptides Derived from Bacillus spp.: A Comprehensive Review.","authors":"Jiaqi Zhang, Xinmiao Yang, Jiajia Qiu, Wen Zhang, Jie Yang, Jinzhi Han, Li Ni","doi":"10.1007/s12602-024-10447-5","DOIUrl":"https://doi.org/10.1007/s12602-024-10447-5","url":null,"abstract":"<p><p>This paper provides a comprehensive review of antimicrobial peptides (AMPs) derived from Bacillus spp. The classification and structure of Bacillus-derived AMPs encompass a diverse range. There are 89 documented Bacillus-derived AMPs, which exhibit varied sources, amino acid sequences, and molecular structures. These AMPs can be categorized into classes I, Ia, IIa, IIb, IIc, and IId. The synthesis pathway of the AMPs primarily involves either ribosomally synthesized or non-ribosomally synthesized approaches. Additionally, the antimicrobial activity of these AMPs is versatile, targeting bacteria, fungi, and viruses, through disrupting intracellular DNA and the cell wall and membrane, as well as modulating immune responses. Moreover, the Bacillus-derived AMPs demonstrate promising application in the pharmaceutical industry, environmental protection, food preservation, and bio-control in agriculture. The commonly employed strategies for enhancing the production of Bacillus-derived AMPs involve optimizing cultivation conditions, implementing systems metabolic engineering, employing genome shuffling techniques, optimizing promoters, and improving expression host optimization. This review can provide a valuable reference for comprehending the current research status on advancements and sustainable production of Bacillus-derived AMPs.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}