Probiotics and Antimicrobial Proteins最新文献

{"title":"High Throughput Meta-analysis of Antimicrobial Peptides for Characterizing Class Specific Therapeutic Candidates: An In Silico Approach.","authors":"Anwesh Pandey, Raji Rajesh Lenin, Sumeet Patiyal, Piyush Agrawal","doi":"10.1007/s12602-025-10596-1","DOIUrl":"https://doi.org/10.1007/s12602-025-10596-1","url":null,"abstract":"<p><p>The increasing incidence of antimicrobial resistance is becoming a serious concern worldwide and requires newer drugs. Recent evidence has shown growing interest in peptide-based therapeutics. Here, we performed a meta-analysis of nearly 867,000 predicted antimicrobial peptides and assessed their antibacterial (ABPs), antifungal (AFPs), and antiviral (AVPs) activity. We created high-quality, class-specific datasets and performed several computational analyses. Composition analysis revealed enrichment of aliphatic (V, A, I, and L) and positively charged (K and R) amino acids in ABPs: aliphatic (G, I), basic (K and R), and aromatic amino acids (F) in AFPs and sulfur containing (M) and aliphatic amino acids (V, I, and L) in AVPs. We observed significant differences in the molecular weight, charge, isoelectric point, and instability index of the peptides among three classes. We observed AFPs possessing the highest molecular weight and ABPs showing the highest charge and isoelectric point, whereas instability index was found to be comparable among the three classes. Motif analysis shows enrichment of unique motifs such as \"VRVR\" and \"AKKPA\" in ABPs, \"DFFAI\" and \"FFAI\" in AFPs, and \"VVV\" and \"IM\" in AVPs. We further developed seven distinct machine learning models to predict peptide activity where ExtraTree model achieved the highest AUROC of 0.98 in classifying ABPs and non-ABPs, 0.99 for classifying AFPs and non-AFPs, and 0.99 for classifying AVPs and non-AVPs on an independent dataset. To assist scientific community, we have provided the dataset and models at our GitHub page ( https://github.com/agrawalpiyush-srm/AMP_MetaAnalysis ). Subsequent filtering of peptides based on moonlighting properties (toxicity, allergenicity, cell-penetrating ability, half-life, and secondary structure) yielded a list of peptides that exhibit substantial therapeutic potential. We further selected the top ten peptides in each class, predicted their 3D structures using ColabFold embedded in ChimeraX1.8 software and performed molecular docking analysis with a pathogenic protein selected from an organism in each class using HDOCK webserver. Docking studies demonstrated strong interaction between peptides and the proteins. Lastly, we proposed list of peptides with high therapeutic potential in each class.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Fecal Bacteroides Strains and Discovery of Bacteroides eggerthii S13-F8 with Protective Effects Against Chemotherapy-Induced Diarrhea.","authors":"Yi Zhao, Zhengqun Dai, Yanmei Lang, Rui Li, Hongyu Zheng, Jiajia Mi, Xiu He, Jin Liu, Rong Xiang, Xueran Mei, Yang Liu, Yantang Wang, Huijie Guo, Qian Yang, Ke Ren, Tai Yang","doi":"10.1007/s12602-025-10595-2","DOIUrl":"https://doi.org/10.1007/s12602-025-10595-2","url":null,"abstract":"<p><p>Chemotherapy-induced diarrhea (CID) is a frequent gastrointestinal side effect in cancer patients, particularly associated with the use of 5-fluorouracil (5-FU). This study aimed to isolate multiple Bacteroides strains from the feces of healthy individuals and identify Bacteroides eggerthii (B. eggerthii) S13-F8 as the optimal candidate for alleviating CID. Whole-genome sequencing of B. eggerthii S13-F8 was conducted to uncover its functional characteristics and explore the potential mechanisms underlying its protective effects against CID. The anti-CID efficacy of B. eggerthii S13-F8 was assessed using multiple parameters, including diarrhea severity, food intake, and body weight changes. Comprehensive analyses, including blood tests, intestinal histopathology, colon transcriptomics, and fecal metagenomics, were performed to elucidate its underlying mechanisms. In a 5-FU-induced mouse model, B. eggerthii S13-F8 significantly alleviated weight loss and diarrhea. Histological examination revealed that B. eggerthii S13-F8 preserved the villus height-to-crypt depth (V/C) ratio and protected goblet cells in colonic tissues. Gene expression analysis showed that B. eggerthii S13-F8 upregulated protective markers, such as Aqp8, Slc26a3, and mucin-related genes (TFF3, FCGBP, and Muc2), while downregulating pro-inflammatory mediators, including IL-1α, IL-22, and Cxcl2. Furthermore, B. eggerthii S13-F8 modulated gut microbiota composition by suppressing pathogenic bacteria (Pseudomonas aeruginosa, Salmonella, γ-Proteobacteria, and Shigella) and enriching beneficial taxa, such as Lactobacillus and Akkermansia muciniphila. In conclusion, B. eggerthii S13-F8 demonstrates significant potential in mitigating severe diarrhea caused by 5-FU chemotherapy, providing a strong foundation for its development as a live biotherapeutic for CID treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactic Acid Bacteria Strains Isolated from Jerusalem Artichoke (Helianthus tuberosus L.) Tubers as Potential Probiotic Candidates.","authors":"Carolina Iraporda, Irene A Rubel, Ana A Bengoa, Guillermo D Manrique, Graciela L Garrote, Analía G Abraham","doi":"10.1007/s12602-025-10594-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10594-3","url":null,"abstract":"<p><p>The search for probiotic candidates is an area that accompanies the world trend of development of novel probiotic strains and new products. In recent years, unconventional sources of potential probiotic bacteria have been studied. Furthermore, there has been an increasing interest in non-dairy and plant-based probiotic foods, currently being considered as a priority for the food industry. The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from Jerusalem artichoke tubers. These strains were characterized by in vitro tests for their biochemical and probiotic properties and safety aspects. The results demonstrated that the LAB strains isolated exhibited a survival rate after acid exposure exceeding 90%, maintained viability above 88% under simulated gastrointestinal conditions, and the autoaggregation capacity ranged from 61 to 81%. Additionally, the strains showed no hemolytic activity and were sensitive to antibiotics. The isolates also downregulated the proinflammatory responses and showed antimicrobial activity against E. coli and B. cereus. The bacilli strains showed a high similarity with Lentilactobacillus kosonis and Lentilactobacillus curieae. Hence, these strains revealed potential probiotic in vitro characteristics that position them to be used in plant-based functional food. This strategic exploration of probiotic bacteria sourced from vegetables not only enhances the diversity of available strains-both taxonomically and functionally-but also fosters the development of sustainable, plant-based probiotic applications.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics Mixture, Prohep: a Potential Adjuvant for Low-Dose Sorafenib in Metabolic Dysfunction-Associated Steatotic Liver Disease-Associated Hepatocellular Carcinoma Suppression Through Modulating Gut Microbiota.","authors":"Fangfei Zhang, Emily Kwun Kwan Lo, Congjia Chen, Jetty Chung-Yung Lee, Felicianna, Marsena Jasiel Ismaiah, Hoi Kit Matthew Leung, Dorothy Hin Lam Tsang, Hani El-Nezami","doi":"10.1007/s12602-025-10593-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10593-4","url":null,"abstract":"<p><p>Targeting gut microbiota is an innovative approach to mitigate the development of metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma (MASLD-HCC). This study aims to investigate the effects of Prohep, a probiotic mixture, both as a prophylactic measure and as an adjuvant therapy for low-dose sorafenib. A MASLD-HCC mice model was established by diethylnitrosamine (DEN) injection with feeding of a high-fat high-cholesterol (HFHC) diet. Gut microbiome profiles were later identified through shotgun sequencing. Our findings demonstrated that Prohep supplementation effectively suppressed MASLD-HCC development in mice. This protective effect was attributed to the modulation of gut microbiota and the increased production of short-chain fatty acids (SCFAs), propionate, and valerate. Prohep also activated AMPK, which decreased lipogenesis, reduced lipid uptake, and enhanced antioxidant enzyme expressions. Additionally, the cancer proliferation pathway PI3K/mTOR was inhibited in response to Prohep treatment. As an adjuvant therapy, Prohep improved the efficacy of low-dose sorafenib, as indicated by reduced tumor counts, alleviated inflammation, and increased hepatic superoxide dismutase (SOD) expression. The combination led to enhanced butyrate production, contributing to the overall therapeutic effects, thanks to the gut microbiota modulatory effects of Prohep. These results underscore Prohep's anti-tumorigenic properties and its potential to enhance the therapeutic outcomes of low-dose sorafenib in MASLD-HCC treatment. The study highlights the importance of gut microbiota modulation for developing effective neoadjuvant therapies and long-term management strategies for MASLD-HCC.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Vegetables as a Source of Psychobiotics: A Review of the Evidence for Mental Health Benefits.","authors":"Eman Shawky, Shelini Surendran, Rasha M Abu El-Khair","doi":"10.1007/s12602-025-10592-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10592-5","url":null,"abstract":"<p><p>The human gut microbiome, comprised of trillions of microorganisms, plays a pivotal role in both physical and mental health. Recent research underscores the intriguing connection between gut bacteria and mental well-being, leading to the emergence of psychobiotics-microbes with mental health benefits. This review aims to explore fermented vegetables, a traditional dietary staple experiencing renewed interest, as a potential source of psychobiotics. Fermentation alters the microbial composition of vegetables, enriching them with beneficial bacteria such as Lactobacillus and Bifidobacterium. Various fermented vegetables, including kimchi, sauerkraut, and tempeh, host distinct bacterial communities. The review investigates how these psychobiotics may impact mental health through the gut-brain axis, a communication network between the gut and the central nervous system. Possible mechanisms encompass neurotransmitter modulation (e.g., serotonin, GABA), inflammation reduction and immunity modulation, and stress response enhancement through the hypothalamic-pituitary adrenal (HPA) axis. Clinical studies exploring the influence of fermented vegetables on mental health outcomes, including anxiety, depression, and cognitive function, are critically evaluated. The review assesses the efficacy of different fermented vegetables and probiotic strains while recognizing limitations in existing research and the necessity for further investigation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The vital role of Lactobacillus sp. in vaginal health: Implications for enhanced prophylactic research.","authors":"Shreaya Das, Bolin Kumar Konwar","doi":"10.1007/s12602-025-10574-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10574-7","url":null,"abstract":"<p><p>Genital opening or vaginal canal (VC) in women has different embryological origin than the entire female reproductive system. The microenvironment of the VC post puberty is everchanging due to the hormonal fluctuations in a woman's body. However, the vaginal canal maintains a defined microbiota with higher population of inherent lactic acid bacteria (LAB) species under healthy conditions. The preservation of the beneficial flora in the genital area is dependent on genetic, social, and habitual factors. The understanding and practice of these factors prevents the commencement of various vaginal infections/vaginitis. Common vaginal infections have known antibiotic treatments; however, treatments of few infections are still unavailable. The study of sexual health is scarce in India due to social and economic factors, with less number of studies from various parts of the country. Thus, there is a necessity of new-age prophylactic solutions in such developing countries. This review highlights the origin of the female reproductive system and describes how the hormonal change initiates unique microenvironment development. Later, we have elaborately discussed the precautionary contribution of lactic acid bacteria and their unexplored commercial utilization, which in turn would help in various ways to improve the standards of reproductive hygiene products.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus acidophilus-Derived Cell-Free Supernatant Augments Methylene Blue-Mediated Photodynamic Inactivation Efficacy Against Methicillin Resistant Staphylococcus aureus Planktonic Suspension and Biofilms.","authors":"Sai Sarbani Mund, Sourabrata Chakraborty, Chandrachur Mukherjee, Khageswar Sahu, Shovan Kumar Majumder","doi":"10.1007/s12602-025-10558-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10558-7","url":null,"abstract":"<p><p>Infections caused by antimicrobial drug-resistant (AMR) pathogens like methicillin-resistant Staphylococcus aureus (MRSA) have triggered global scale search for innovative, clinically viable and cost affordable alternatives that do not generate resistance. Antimicrobial photodynamic therapy (aPDT) and postbiotics are innovative alternatives to circumvent AMR pathogens. However, MRSA biofilms reduce microbial susceptibility to aPDT and postbiotics. Integrating both modalities can be a novel MRSA anti-biofilm therapeutics avenue, but further studies are needed to validate the efficacy and applicability. We investigated the potentiality of methylene blue (MB)-mediated aPDT (MB-aPDT) followed by Lactobacillus acidophilus-derived cell-free supernatant (LA-CFS) treatment on MRSA planktonic culture, pre-formed biofilms, biofilm initiation, and maturation. Compared to aPDT or LA-CFS monotherapy, MB-aPDT followed by LA-CFS (20 and 40% v/v) treatment for 2 h leads to augmentation in inactivation of MRSA planktonic culture and CFU data correlates with flow cytometry data (R² = 0.87). Atomic force microscopy (AFM) unravels different pattern of morphological alterations elicited by aPDT or LA-CFS, but extensive cellular damage and cell fragmentation results from the combinatorial treatment. The determinants of LA-CFS-based augmented action seem to be pH labile. Further, destruction of preformed biofilm shows the following trend: aPDT < LA-CFS < aPDT + LA-CFS. Furthermore, MRSA surviving LA-CFS or aPDT can either adhere to the substratum or form loose biofilms, respectively, but the combinatorial regimen remarkably inhibits adherence and subsequent biofilm initiation, as well as maturation. The augmentation of MRSA inactivation using aPDT-postbiotics can be a new therapeutic avenue for the management of MRSA biofilm-associated infections.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Chemically Modified Antimicrobial Peptides and Their Sub-functional Activities Using Hybrid Features.","authors":"Yujie Yao, Daijun Zhang, Henghui Fan, Ting Wu, Yansen Su, Yannan Bin","doi":"10.1007/s12602-025-10575-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10575-6","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) demonstrate a broad spectrum of activities against various pathogens, thereby offering a promising strategy to mitigate the urgent challenge of antimicrobial resistance. Recent studies indicate that chemically modified AMPs (cmAMPs), which contain chemically modified amino acids, have the potential to alleviate the adverse effects commonly associated with conventional AMPs. Nevertheless, there remains a notable deficiency in computational methods specifically designed for the analysis and prediction of cmAMPs and their sub-function predictions. In this study, we proposed a two-layer model, termed as iCMAMP, aimed for the identification of cmAMPs and their sub-functional activities. The first layer, referred to as iCMAMP-1L, integrates three categories encompassing seven distinct groups of features, in conjunction with an ensemble method designed at enhancing predictive accuracy for cmAMPs. This ensemble approach effectively extracts relevant insights from a heterogeneous array of features sets while addressing potential dimensionality challenges. On the test dataset, iCMAMP-1L achieved an ACC of 0.934 and an MCC of 0.868, representing improvements of 3.4% and 6.8%, respectively, over AntiMPmod, which is the sole existing method for predicting cmAMPs. A comparative analysis between cmAMPs and their corresponding AMPs revealed that chemical modifications can significantly reduce hemolysis and toxicity associated with AMPs, while the functional characteristics of the peptides are primarily determined by their sequences. The second layer of our model, designated as iCMAMP-2L, employed a multi-label classification approach to predict the sub-functional activities of cmAMPs, with a specific focus on the dipeptide composition-based features. On the test dataset, iCMAMP-2L achieved an Accuracy of 0.390 and an Absolute true of 0.621. The data and Python code used in the iCMAMP model are available at https://github.com/swicher123/iCMAMP/tree/master .</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico bioprospecting of the Neotropical Plant Mandacaru (Cereus) for antimicrobial properties.","authors":"João A Teodoro, Marcus V X Senra, Danilo T Amaral","doi":"10.1007/s12602-025-10580-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10580-9","url":null,"abstract":"<p><p>The mandacaru is a cactus species complex widely known in Brazil, with extensive applications in medicinal, food, and agricultural fields. Although it is used medicinally by traditional populations, to treat several diseases, knowledge about its biomolecules of biotechnological potential is still limited, specifically regarding antimicrobial and healing properties. The bacterial resistance to conventional antibiotics presents a significant challenge in modern medicine. In light of this scenario, bioprospecting mandacaru for biotechnological applications as an antimicrobial has emerged as a new and promising research area. In this study, transcriptomic data from three Cereus species (C. fernambucensis, C. hildmannianus, and C. jamacaru) were combined with bioinformatic approaches, including protein modeling, molecular docking, and molecular dynamics simulations, to identify proteins with therapeutic potential for treating wound infections. Our findings highlighted peptides as particularly promising antimicrobial agents, demonstrating efficacy against a range of pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi. Those peptides showed strong interactions with the streptolydigin and sodium ligands, with the streptolydigin ligand emerging as the most promising for enhancing antimicrobial activity. Molecular dynamics revealed that while CF15 exhibited limited stability, CF267, CF48, CH167, and CH176 displayed superior stability, positioning them as the most promising candidates for further investigation. Future work will focus on synthesizing these peptides and evaluating their antimicrobial properties through in vitro and in vivo analyses, to develop them into potent therapeutic agents.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the Microbiome: CRISPR-Based Gene Editing and Antimicrobial Peptides in Combating Antibiotic Resistance and Cancer.","authors":"Radwa A Amen, Yaser M Hassan, Rawan A Essmat, Rana H Ahmed, Marwan M Azab, Nadia R Shehata, Mariam M Elgazzar, Wael M El-Sayed","doi":"10.1007/s12602-025-10573-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10573-8","url":null,"abstract":"<p><p>The growing crisis of antibiotic resistance and the increasing incidence of cancer have prompted the exploration of innovative approaches, such as gene editing and antimicrobial peptides (AMPs). The human microbiome is integral to various aspects of health, disease, and therapeutic development, influencing metabolic pathways, immune function, and pathogen resistance. Recent advances in gene editing technologies, particularly CRISPR (clustered regularly interspaced short palindromic repeats), have opened new avenues for leveraging the microbiome to address complex medical challenges, including combating multidrug-resistant pathogens and cancer. The microbiome plays a crucial role in combating antibiotic resistance by modulating microbial communities, influencing pathogen survival and susceptibility to treatments. This review explores the microbiome's dynamic role in metabolic regulation, its contribution to cancer management, and how AMPs help maintain homeostasis and exhibit emerging anticancer properties, supported by both preclinical findings and clinical evidence. Additionally, CRISPR-based microbiome engineering offers potential to enhance host-microbiome interactions, optimizing therapeutic outcomes. The integration of microbiome metagenomics and proteomics has led to the discovery of novel AMPs with targeted anticancer effects. Innovative strategies, such as engineered probiotics and CRISPR-based microbiome engineering, present exciting prospects for next-generation therapies. Despite these advances, the translation of microbiome-based therapies into clinical settings remains challenging due to ethical, regulatory, and ecological hurdles. This review underscores the transformative potential of microbiome-based interventions, emphasizing the role of personalized medicine in maximizing therapeutic efficacy. Furthermore, we also address critical research gaps, limitations, and future directions, including optimizing AMP stability, delivery, and bioavailability, as well as overcoming the regulatory and ethical challenges in clinical translation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}