Probiotics and Antimicrobial Proteins最新文献_第10页

Kefir and Its By-Products Supplementation Reduces Inflammation and Oxidative Stress, Improves Intestinal Barrier Integrity, and Modulates the Gut Microbiota in Animal Models of Inflammatory Bowel Disease: A Systematic Review. 在炎症性肠病动物模型中，补充开非尔及其副产物可减少炎症和氧化应激，改善肠道屏障完整性，调节肠道微生物群：一项系统综述

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-03 DOI: 10.1007/s12602-026-10948-5

Maria Alice Spadarotto Neves, Rayanne Santos de Paulo, Josefina Bressan, Solange Silveira Pereira, Ana Claudia Pelissari Kravchychyn, Helen Hermana Miranda Hermsdorff

引用次数: 0

A Bioinformatics Pipeline for Screening Nodule-Specific Cysteine-Rich (NCR) Like Peptides from Trigonella foenum-graecum and Medicago truncatula Genomes. 利用生物信息学途径筛选三角Trigonella foenum-graecum和Medicago truncatula基因组中的结节特异性富半胱氨酸（NCR）样肽。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-03 DOI: 10.1007/s12602-026-10940-z

Sneha Bidvi, Rajendra Choure, Manohar Padul, Rupendra Jadhav, Sayli Mandavkar, Akshada Bhadane, Mrunal Posam

引用次数: 0

GK-11: a Novel Cationic Peptide with Antibiofilm Activity against Staphylococcus aureus and Pseudomonas aeruginosa. GK-11：一种具有抗金黄色葡萄球菌和铜绿假单胞菌活性的阳离子肽。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-03 DOI: 10.1007/s12602-026-10963-6

Simay Aldağ, Güler Tuba Buğdacı, Şeymanur Çobanoğlu, Erdem Erkengez, Mehmet Enes Arslan, Abdurrahim Kadı, Serkan Örtücü, Mesut Taşkın, Ayşenur Yazıcı

引用次数: 0

The Role of Functional Feed in Modulating Fish Gut Microbiome to Enhance Resistance Against Aquaculture Pathogens. 功能饲料在调节鱼类肠道微生物群增强对水产养殖病原体抗性中的作用。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-07-22 DOI: 10.1007/s12602-025-10660-w

Eswar Marcharla, A Vishnuprasadh, Lalitha Gnanasekaran, Saranya Vinayagam, Thanigaivel Sundaram, Swamynathan Ganesan

{"title":"The Role of Functional Feed in Modulating Fish Gut Microbiome to Enhance Resistance Against Aquaculture Pathogens.","authors":"Eswar Marcharla, A Vishnuprasadh, Lalitha Gnanasekaran, Saranya Vinayagam, Thanigaivel Sundaram, Swamynathan Ganesan","doi":"10.1007/s12602-025-10660-w","DOIUrl":"10.1007/s12602-025-10660-w","url":null,"abstract":"The gut microbiome, comprising of diverse microbial species, plays a critical role in the immunological responses and physiological functions of fish. Functional feed components such as probiotics, prebiotics, immunostimulants (e.g. β-glucans), and bioactive compounds (e.g. phenolic compounds and terpenes) enhance disease resistance and overall health. This review highlights the diversity and composition of the fish gut microbiome and its significant role in immune modulation. It examines the ability of functional feed components, microbiome-associated metabolites, including antimicrobial peptides, bile acids, and short-chain fatty acids (SCFAs), to influence the fish immune system. Also, it focuses on the role of extracellular vesicles and quorum-sensing molecules in modulating gut health. Furthermore, high-throughput metabolomics techniques, such as gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy, are utilised to analyse gut microbiome metabolites and optimise functional feeds. These characterisation techniques effectively detect the metabolites released in the microbiota for better feed utilisation. Functional feeds enhance growth performance by helping the fish to maintain stable gut microbiota, thus reducing the dependency on antibiotics. This review clearly establishes the transformative potential of different functional feeds for enhancing and promoting sustainable aquaculture practices. However, challenges such as horizontal gene transfer and long-term ecological impacts of microbiome alterations persist. Also, economic feasibility, regulations, and biosafety considerations may affect the widespread use of these functional feeds. Future studies should focus on refining feed formulations, understanding host-microbiome interactions, and leveraging advanced omics technologies to ensure ecological and economic sustainability in aquaculture systems.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"3010-3039"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Adipogenic Effects of Probiotic Lactiplantibacillus pentosus THF59 via Inhibition of Differentiation and Promotion of Apoptosis in 3T3-L1 Cells. 益生菌乳酸杆菌THF59通过抑制3T3-L1细胞分化和促进细胞凋亡的抗脂肪作用

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-07-11 DOI: 10.1007/s12602-025-10663-7

Qiwen Zheng, Trang Thi Minh Nguyen, Xiangji Jin, Gyeong-Seon Yi, Tae-Hoo Yi

{"title":"Anti-Adipogenic Effects of Probiotic Lactiplantibacillus pentosus THF59 via Inhibition of Differentiation and Promotion of Apoptosis in 3T3-L1 Cells.","authors":"Qiwen Zheng, Trang Thi Minh Nguyen, Xiangji Jin, Gyeong-Seon Yi, Tae-Hoo Yi","doi":"10.1007/s12602-025-10663-7","DOIUrl":"10.1007/s12602-025-10663-7","url":null,"abstract":"This study investigated the probiotic properties and anti-adipogenic activities of Lactiplantibacillus pentosus THF59, isolated from blueberries. THF59 exhibited several probiotic traits, including antimicrobial activity, tolerance to simulated gastric juice and bile salts, non-pathogenicity, and strong adhesion to intestinal epithelial Caco-2 cells. To evaluate its anti-adipogenic potential, MDI-induced 3T3-L1 preadipocytes were treated with THF59 cell-free supernatant (CFS). At the highest tested concentration (100 μg/mL), THF59 CFS significantly reduced intracellular lipid accumulation by 68.40%, as evidenced by Oil Red O staining. It also decreased intracellular reactive oxygen species (ROS) levels by 27.45%, potentially suppressing adipogenic differentiation by disrupting ROS-mediated early signaling events. Moreover, THF59 alleviated MDI-induced S-phase cell cycle arrest, suggesting interference with early-stage preadipocyte differentiation. At this concentration, THF59 CFS significantly downregulated key adipogenic transcription factors involved in various stages of differentiation, including SREBP-1 (68.91%), PPARγ (50.75%), C/EBPα (41.99%), and AP-2 (37.44%). These findings indicate that THF59 suppressed adipogenesis through multi-level regulatory interference, from early commitment to terminal differentiation. Notably, THF59 also promoted late-stage apoptosis in mature adipocytes by 6.55%, representing an additional lipid-lowering mechanism through the removal of lipid-storing cells. In conclusion, THF59 exerted anti-adipogenic effects in MDI-induced 3T3-L1 preadipocytes by inhibiting differentiation and promoting apoptosis, supporting its potential as a functional probiotic candidate for obesity prevention.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2025-2036"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Genetics and Probiotic Mechanisms of Saccharomyces cerevisiae var. boulardii. 酿酒酵母博氏变种的分子遗传学及益生菌机制。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-07-24 DOI: 10.1007/s12602-025-10634-y

Tiew-Yik Ting, Wei-Jing Lee, Hoe-Han Goh

{"title":"Molecular Genetics and Probiotic Mechanisms of Saccharomyces cerevisiae var. boulardii.","authors":"Tiew-Yik Ting, Wei-Jing Lee, Hoe-Han Goh","doi":"10.1007/s12602-025-10634-y","DOIUrl":"10.1007/s12602-025-10634-y","url":null,"abstract":"Saccharomyces cerevisiae var. boulardii (Sb) is a S. cerevisiae (Sc) strain that has been widely used in the treatment of gastrointestinal diseases due to its unique probiotic properties. The key genomic differences that distinguish Sb from Sc include the tetrasomy of chromosome XII, the absence of intact transposon-yeast (Ty) elements, and variations in the copy number of specific genes. These genomic variations may contribute to enhanced thermotolerance, increased acid resistance, and elevated acetate production, collectively supporting its probiotic functions. The probiotic mechanisms of Sb are mediated through luminal actions, mucosal actions, and trophic effects. Its luminal activity involves neutralizing pathogen toxins via the secretion of proteins and inhibiting pathogen growth through the production of short-chain fatty acids (SCFAs). Additionally, Sb modulates gut microbiota composition by fostering symbiotic relationships, thereby increasing the abundance of beneficial microbes and SCFA levels to promote gut health. The mucosal action of Sb promotes anti-inflammatory responses by regulating the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. Meanwhile, its trophic effects, driven by polyamine production, enhance the function of intestinal epithelial cells. Recent findings further suggest that Sb may serve as a potential adjuvant therapy for brain disorders by modulating the gut-brain axis (GBA) to attenuate neuroinflammation. With continued multidisciplinary research, Sb is well-positioned to advance the biotherapeutic landscape. This review aims to synthesize recent advances in the genetics and probiotic mechanisms of Sb, with particular emphasis on its modulatory effects on the GBA.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"3040-3054"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic Acid Bacteria-Derived Secondary Metabolites: Emerging Natural Alternatives for Food Preservation. 乳酸菌衍生的次生代谢物：新兴的食品保存天然替代品。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-08-02 DOI: 10.1007/s12602-025-10672-6

Riza Jane S Banicod, Nazia Tabassum, Aqib Javaid, Young-Mog Kim, Fazlurrahman Khan

{"title":"Lactic Acid Bacteria-Derived Secondary Metabolites: Emerging Natural Alternatives for Food Preservation.","authors":"Riza Jane S Banicod, Nazia Tabassum, Aqib Javaid, Young-Mog Kim, Fazlurrahman Khan","doi":"10.1007/s12602-025-10672-6","DOIUrl":"10.1007/s12602-025-10672-6","url":null,"abstract":"Lactic acid bacteria (LAB) are extensively utilized in the food industry to improve nutritional content, sensory attributes, and shelf life of various food products through the synergistic action of several antimicrobial metabolites and ecological processes. The synthesis of organic acids lowers the pH of the food matrix, destabilizing microbial membranes and rendering them vulnerable to antimicrobial agents such as bacteriocin, hydrogen peroxide, diacetyl, peptides, and fatty acids. These create an environment that is inhospitable to foodborne pathogens and spoilage microorganisms, which are amplified by the ability of LAB to rapidly colonize diverse ecological niches through efficient competition for nutrients and space. Despite their promising applications, challenges remain in optimizing LAB-based preservation systems for large-scale food production, including strain variability, stability concerns during storage, and regulatory obstacles. The application of LAB-derived secondary metabolites in food preservation, particularly biosynthesis, underlying action mechanisms, inhibitory factors, and impacts on food safety, quality, and stability, was eloquently explained in detail in this review paper. These secondary metabolites have also been employed with other bioactive agents and processing technologies as a synergistic approach to control food spoilage pathogens. Moreover, the challenges and limitations associated with their industrial adoption, synergistic application with emerging food processing technologies, and future prospects in the food industry have also been discussed. Harnessing the full potential of LAB in food preservation presents promising opportunities for developing innovative and sustainable substitutes for traditional chemical preservatives, which corresponds with the increasing consumer preference for safer, clean-label, and eco-friendly food products.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"3113-3150"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Antimicrobial Efficacy of Nisin-Loaded Particles with Different Surface Charges. 不同表面电荷负载乳酸链球菌素颗粒的抗菌效果评价。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-08-27 DOI: 10.1007/s12602-025-10737-6

Rūta Gruškienė, Alisa Gricajeva, Jolanta Sereikaitė

{"title":"Evaluation of the Antimicrobial Efficacy of Nisin-Loaded Particles with Different Surface Charges.","authors":"Rūta Gruškienė, Alisa Gricajeva, Jolanta Sereikaitė","doi":"10.1007/s12602-025-10737-6","DOIUrl":"10.1007/s12602-025-10737-6","url":null,"abstract":"Nisin is a well-known bacteriocin used for food preservation. Encapsulation technologies can help increase its stability and protect its interaction with food components. The surface charge of nisin-loaded particles usually depends on that of the carrier. However, the effect of the positive and negative charge of the particles on the expression of antimicrobial activity is not fully understood. This study aims to determine how the surface charge of nisin-loaded particles influences their antimicrobial activity against Staphylococcus aureus and Streptococcus pyogenes. Nisin-loaded particles were fabricated using three types of pectin, such as high methoxyl, low methoxyl pectin, and pectic acid, at the final concentrations of pectin and nisin of 0.2 mg/mL and 0.1 mg/mL, respectively, followed by coating with chitooligosaccharides at 0.025 mg/mL and 0.3 mg/mL. Nisin release from particles was analysed at pH values 2, 6, and 8. The Korsmeyer-Peppas model provided the best description of nisin release. The antibacterial effect of nisin-loaded low methoxyl pectin particles, uncoated and coated with chitooligosaccharides, was investigated on Gram-positive bacterial strains S. pyogenes ATCC 12384 and S. aureus ATCC 25932. The zeta-potentials of nisin-loaded particles uncoated and coated with 0.025 mg/mL of chitooligosaccharides were - 20.6 ± 5.8 and - 18.1 ± 0.7 mV, respectively. The particles coated with 0.3 mg/mL of chitooligosaccharides were positively charged, with a zeta potential of 20.0 ± 5.0 mV. The study showed that there was no significant difference (p > 0.05) in the antibacterial effect of particles with the same but opposite charge, that is, negative and positive. This finding broadens the possibilities for nisin formulations and the design of new delivery systems.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2776-2785"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Three Microbiome-Based Therapies on Loperamide-Induced Constipation in Mice by Restoring Intestinal Motility and Modulating the Intestinal Microbiota. 三种基于微生物组的治疗方法通过恢复肠道动力和调节肠道微生物群对洛哌丁胺诱导的小鼠便秘的影响。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-08-27 DOI: 10.1007/s12602-025-10732-x

Xinfeng Bai, Lin Xu, Kai Wang, Lei Chen, Tianyu Bai, Peipei Li, Weihua Chu, Chao Song

{"title":"Effects of Three Microbiome-Based Therapies on Loperamide-Induced Constipation in Mice by Restoring Intestinal Motility and Modulating the Intestinal Microbiota.","authors":"Xinfeng Bai, Lin Xu, Kai Wang, Lei Chen, Tianyu Bai, Peipei Li, Weihua Chu, Chao Song","doi":"10.1007/s12602-025-10732-x","DOIUrl":"10.1007/s12602-025-10732-x","url":null,"abstract":"Constipation is a common gastrointestinal disorder, often associated with dysbiosis of the intestinal microecology. The treatments for constipation are various, and microbiome-based therapies provide supplementary or alternative options for its management. This study compared the therapeutic effects of three microbiome-based therapies, including probiotics, prebiotics, and traditional Chinese medicine (TCM), with the agonist mosapride on loperamide-induced constipation in mice, focusing on their restorative effects on intestinal motility and intestinal microecology. The results showed that mosapride had the most significant improvement in gut motility and key motility hormones, and also significantly restored gut microbiota. Probiotics significantly improved the levels of the motility hormones motilin (MTL) and substance P (SP) and significantly altered the composition of the microbiota. TCM significantly increased gut motility and was most effective in improving gut microbiota composition and short-chain fatty acid (SCFA) content. Inulin, on the other hand, had the least effect on defecation phenotype, intestinal motility, and restoration of intestinal microecology. Overall, this preclinical study in mice provides experimental insights into the therapeutic efficacy and potential mechanisms of these methods, which may contribute to the appropriate selection of candidate therapies for human constipation subtypes characterized by motility-microbiota axis dysfunction. In addition, the research highlights the critical importance of gut microbiota and their metabolites in the treatment of constipation and their potential as therapeutic indicators.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2759-2775"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Synbiotics Consisting of Catechin and Probiotic Bacteria: Exploring Aggregation, Adhesion, Antioxidant, and Anticancer Effects. 新兴的由儿茶素和益生菌组成的合成菌：探索聚集、粘附、抗氧化和抗癌作用。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2026-03-01 Epub Date: 2025-06-20 DOI: 10.1007/s12602-025-10631-1

Sena Davran Bulut, Buse Nur Derebası, Sevilay Günay, Yavuz Erden, Hasan Ufuk Celebioglu, Mustafa Ümit Ünal

{"title":"Emerging Synbiotics Consisting of Catechin and Probiotic Bacteria: Exploring Aggregation, Adhesion, Antioxidant, and Anticancer Effects.","authors":"Sena Davran Bulut, Buse Nur Derebası, Sevilay Günay, Yavuz Erden, Hasan Ufuk Celebioglu, Mustafa Ümit Ünal","doi":"10.1007/s12602-025-10631-1","DOIUrl":"10.1007/s12602-025-10631-1","url":null,"abstract":"Probiotic bacteria are microorganisms that confer health benefits on the host, when administered in adequate amounts. Catechin, naturally found in nature and human diet, has strong antioxidant, antibacterial, and anticancer potentials. Phenolic compounds and probiotic bacteria are found together in gastrointestinal tract (GIT), and their potential synbiotic interactions are not fully understood. The present study aims to investigate the probiotic properties effects of catechin on (bacterial growth, auto-aggregation, co-aggregation) on well-known probiotic bacteria Lactobacillus acidophilus LA-5 (LA-5) and Lacticaseibacillus rhamnosus GG (LGG), and in vitro cytotoxic effects of the synbiotic combination of catechin and probiotic bacteria on colon cancer cells Caco-2. Results show that catechin contributed to bacterial growth of LA-5, auto-aggregation of LA-5, co-aggregation of LA-5 with Staphylococcus aureus. Catechin increased the adhesion potential of LA-5 and LGG by 6% and 5%, respectively. In DPPH scavenging activity, LGG grown with catechin exerted 30% more antioxidant potential compared the LGG. Moreover, LA-5 grown in the presence of catechin led to higher cell death of Caco-2 when compared to control while it did not influence the genotoxicity property of LA-5. However, catechin did not promote the cytotoxicity and genotoxicity properties of LGG. In conclusion, catechin enhanced many physiological properties of probiotic bacteria and thus, synbiotic relationship of catechin and probiotics may offer novel insights into the prevention/treatment of colon cancer.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"1797-1810"},"PeriodicalIF":4.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13013179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0