Probiotics and Antimicrobial Proteins最新文献

{"title":"Potential of Synbiotics and Probiotics as Chemopreventive Agent.","authors":"Kah Wei Chin, Shing Ching Khoo, Richard Paul Merisha Paul, Vijitra Luang-In, Su Datt Lam, Nyuk Ling Ma","doi":"10.1007/s12602-024-10299-z","DOIUrl":"10.1007/s12602-024-10299-z","url":null,"abstract":"<p><p>Cancer remains a global problem, with millions of new cases diagnosed yearly and countless lives lost. The financial burden of cancer therapy, along with worries about the long-term safety of existing medicines, necessitates the investigation of alternative approaches to cancer prevention. Probiotics generate chemopreventive compounds such as bacteriocins, short-chain fatty acids (SCFA), and extracellular polymeric substances (EPS), which have demonstrated the ability to impede cancer cell proliferation, induce apoptosis, and bolster the expression of pro-apoptotic genes. On the other hand, prebiotics, classified as non-digestible food ingredients, promote the proliferation of probiotics within the colon, thereby ensuring sustained functionality of the gut microbiota. Consequently, the synergistic effect of combining prebiotics with probiotics, known as the synbiotic effect, in dietary interventions holds promise for potentially mitigating cancer risk and augmenting preventive measures. The utilization of gut microbiota in cancer treatment has shown promise in alleviating adverse health effects. This review explored the potential and the role of probiotics and synbiotics in enhancing health and contributing to cancer prevention efforts. In this review, the applications of functional probiotics and synbiotics, the mechanisms of action of probiotics in cancer, and the relationship of probiotics with various drugs were discussed, shedding light on the potential of probiotics and synbiotics to alleviate the burdens of cancer treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"2085-2101"},"PeriodicalIF":4.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of Saccharomyces spp. for Use as Probiotic in Food and Feed: Systematic Review and Meta-analysis.","authors":"Isabel Marie Grambusch, Caroline Schmitz, Cláudia Schlabitz, Rodrigo Gay Ducati, Daniel Neutzling Lehn, Claucia Fernanda Volken de Souza","doi":"10.1007/s12602-024-10331-2","DOIUrl":"10.1007/s12602-024-10331-2","url":null,"abstract":"<p><p>Probiotics, particularly yeasts from the genus Saccharomyces, are valuable for their health benefits and potential as antibiotic alternatives. To be effective, these microorganisms must withstand harsh environmental conditions, necessitating advanced protective technologies such as encapsulation to maintain probiotic viability during processing, storage, and passage through the digestive system. This review and meta-analysis aims to describe and compare methods and agents used for encapsulating Saccharomyces spp., examining operating conditions, yeast origins, and species. It provides an overview of the literature on the health benefits of nutritional yeast consumption. A bibliographic survey was conducted following the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. The meta-analysis compared encapsulation methods regarding their viability after encapsulation and exposure to the gastrointestinal tract. Nineteen studies were selected after applying inclusion/exclusion criteria. Freeze drying was found to be the most efficient for cell survival, while ionic gelation was best for maintaining viability after exposure to the gastrointestinal tract. Consequently, the combination of freeze drying and ionic gelation proved most effective in maintaining high cell viability during encapsulation, storage, and consumption. Research on probiotics for human food and animal feed indicates that combining freeze drying and ionic gelation effectively protects Saccharomyces spp.; however, industrial scalability must be considered. Reports on yeast encapsulation using agro-industrial residues as encapsulants offer promising strategies for preserving potential probiotic yeasts, contributing to the environmental sustainability of industrial processes.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":"1979-1995"},"PeriodicalIF":4.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics and Prebiotics Intervention in Respiratory and Digestive Infections Linked to Covid-19.","authors":"Tahar Amrouche, Sarah Lammi, Djamel Drider","doi":"10.1007/s12602-024-10404-2","DOIUrl":"https://doi.org/10.1007/s12602-024-10404-2","url":null,"abstract":"<p><p>Probiotics and prebiotics have been suggested as natural agents against viral infections and dysbiosis and may encourage clinical applications. This review aims to analyze the main and recent advances related to viral infections such as Covid-19 and its gastrointestinal complications, antiviral immunity generated and possible preventive role that probiotics and/or prebiotics can play in controlling and promoting antiviral immunity. The literature search was performed through a critical analysis of relevant publications reported in PubMed and Scopus databases on clinical trials and assays conducted in vitro on colon cells and in vivo on mice. Some studies using probiotics and prebiotics for the prevention of viral infection in different age groups are discussed. Covid-19 patients have been shown to suffer from gastrointestinal complications in addition to respiratory symptoms due to interactions between the respiratory system and the gastrointestinal tract infected with SARS-CoV-2. Unfortunately, therapies used to prevent (or treat) symptoms of Covid-19 have proven to be of limited effectiveness. In addition, the lack of access to coronavirus vaccines around the world and vaccine hesitancy continue to hamper control of Covid-19. It is therefore crucial to find alternative methods that can prevent disease symptoms. Evidence-based efficacy of certain probiotics (Lactobacillus and Bifidobacterium) that may be useful in viral infections was shown with immunomodulatory properties (pro-inflammatory mediators reduction), promoting antiviral immunity (antibodies production, virus titers) and controlling inflammation (anti-inflammatory effect), as well as viral clearance and antimicrobial potential against opportunistic bacteria (anti-dysbiosis effect). But, available data about clinical application of probiotics in Covid-19 context remain limited and relevant scientific investigation is still in its early stages. Also, evidence for prebiotics potential in this field is limited, since the exact mechanism involved in systemic immune modulation by these compounds is till now unknown. Thus, further research is necessary to explore in the viral infection context the mechanism by which gut and lung interact in the presence of probiotics and prebiotics through more animal and clinical experiments.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Spores and Vegetative Forms of Bacillus subtilis and Bacillus licheniformis on Probiotic Functionality In Vitro and In Vivo.","authors":"Hua Xie, Tianfei Yu, Qiwen Zhou, Kai Na, Shuang Lu, Li Zhang, Xiaohua Guo","doi":"10.1007/s12602-024-10407-z","DOIUrl":"https://doi.org/10.1007/s12602-024-10407-z","url":null,"abstract":"<p><p>The probiotic effects of Bacillus are strain-specific and dependent on both spore and vegetative forms, but the distinct contributions of these forms to probiotic functionality are not well understood. This study aimed to evaluate and compare the impacts of vegetative forms and spores of Bacillus subtilis and Bacillus licheniformis on probiotic functions in vitro and in vivo. We systematically assessed the anaerobic metabolic capabilities and the potential to enhance the intestinal barrier function of four Bacillus strains, leading to the selection of Bacillus subtilis X22 and Bacillus licheniformis N-3 for detailed investigation. Utilizing in vitro fermentation with murine fecal microbiota, we observed that the spores form of Bacillus licheniformis N-3 noticeably positively regulated the gut microbiota under anaerobic conditions. Concurrently, both spore and vegetative forms of Bacillus licheniformis N-3 and Bacillus subtilis X22 demonstrated the ability to prevent pathogen adhesion, reduce inflammation, combat oxidative stress, and promote cellular autophagy to reduce apoptosis in response to enterotoxigenic Escherichia coli (ETEC) infection in the IPEC-J2 cell model. As a facultative anaerobe, Bacillus licheniformis N-3 exhibited a tendency toward superior regulatory capacity in enhancing the anti-infective activity of IPEC-J2 cells in vitro. In the pathogens challenge mouse model, B. licheniformis N-3 effectively preserved the integrity of jejunal tissue and enhanced the expression of glycoproteins in goblet cells. Moreover, B. licheniformis N-3 strengthened the epithelial barrier by increasing the levels of Occludin and Claudin-1 in the jejunum, thus promoting overall intestinal health. This research offers new insights into strain selection and the life cycle utilization of Bacillus probiotics.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Tryptophan Metabolism on the Protective Effect of Weissella paramesenteroides WpK4 in a Murine Model of Chemotherapy-Induced Intestinal Mucositis.","authors":"Gabriele Moreira Guimarães, Karen Costa, César da Silva Santana Moura, Sarah Elisa Diniz Moreira, Joana Mozer Marchiori, Anna Clara Paiva de Menezes Santos, Rafaela Ribeiro Alvares Batista, Celso Martins Queiroz-Junior, Juliana Divina Almeida Raposo, Fernão Castro Braga, Marcelo Vidigal Caliari, Álvaro Cantini Nunes, Caio Tavares Fagundes, Elisabeth Neumann","doi":"10.1007/s12602-024-10413-1","DOIUrl":"https://doi.org/10.1007/s12602-024-10413-1","url":null,"abstract":"<p><p>Dysbiosis is a notable marker of intestinal mucositis, an inflammatory condition induced by antineoplastic chemotherapy. Scientific evidence supports the effectiveness of probiotics in managing dysbiosis associated with intestinal mucositis. It is known that tryptophan metabolism is a regulatory component in the multifactorial phenomenon of mucosal homeostasis. In the face of that, we aimed to investigate if oral administration of Weissella paramesenteroides WpK4, a probiotic candidate strain, has a protective effect in a murine model of intestinal mucositis induced by 5-fluorouracil (5-FU) and if tryptophan metabolism plays any role in this effect. Gavage with viable cells of W. paramesenteroides WpK4 increased intestinal mucus production, regeneration of villi, as well as control of dysbiosis in mice submitted to 5-FU chemotherapy, and resulted in 100% survival, unlike the control saline-treated group, which resulted in 60% survival of mice after mucositis induction. Weissella paramesenteroides WpK4 genome harbors sequences encoding enzymes for tryptophan production and catabolism and can synthesize tryptophan, tryptamine, and indole acetic acid in vitro. Besides, oral administration of WpK4 induced increased expression of molecules involved in tryptophan metabolism in mouse ileum and serum. Notably, simultaneous treatment with alfa-naphthoflavone, an aryl hydrocarbon receptor (AhR) inhibitor, abolished the protective effects exerted by W. paramesenteroides Wpk4, as manifested by a significant decline in body weight, suggesting that treatment with the probiotic strain modulates AhR activation. Our results suggest that tryptophan metabolism is potentially involved in the protective effects caused by oral administration of W. paramesenteroides WpK4 to mice during gut inflammatory conditions induced by 5-FU.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotic Effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on a Murine Model of 5-FU-induced Intestinal Mucositis.","authors":"Rafael de Assis Glória, Tales Fernando da Silva, Tomás Andrade Magalhães Gomes, Kátia Duarte Vital, Simone Odília Antunes Fernandes, Valbert Nascimento Cardoso, Ênio Ferreira, Jean-Mark Chatel, Philippe Langella, Claire Cherbuy, Yves Le Loir, Gwénaël Jan, Éric Guédon, Vasco Ariston de Carvalho Azevedo","doi":"10.1007/s12602-024-10414-0","DOIUrl":"https://doi.org/10.1007/s12602-024-10414-0","url":null,"abstract":"<p><p>Probiotics are live microorganisms that, when administered in adequate amounts, can bring health benefits to the host. Most of these organisms are found naturally in the human gastrointestinal tract. Escherichia coli strains Nissle 1917 (EcN), and CEC15 have shown beneficial effects in murine models of intestinal inflammation, such as colitis and mucositis. The present study evaluated the effects as postbiotic of heat-inactivated and cell-free supernatant preparations of EcN and CEC15 in attenuating 5-fluorouracil (5-FU)-induced intestinal mucositis in mice and compared them with the probiotic effects of the live preparations. BALB/c mice were fed, by daily gavage, with 10¹⁰ CFU of live or inactivated bacteria or with 300 µL of cell-free supernatant for 12 days. On the 10th day, all animals, except for the control group, received an intraperitoneal injection of 5-FU (300 mg/kg). After 72 h of 5-FU administration, animals were euthanized, and the ileum and blood were collected for analysis. Treatments with live and heat-inactivated CEC15 mitigated weight loss, preserved intestinal length, reduced histological damage, maintained goblet cells, decreased neutrophil infiltration, and modulated expression of inflammatory and barrier genes when compared to 5-FU mucositis controls. EcN showed more limited effects. CEC15 upregulated mRNA expression of the mucin MUC2 and tight junction protein TJP1. CEC15 demonstrated protective effects against 5-FU-induced mucositis, whether administered with live, heat-inactivated, or cell-free supernatant. This suggests that CEC15 mediates a protective response via secreted metabolites and does not require viability. The postbiotic forms of CEC15 present advantages for use in immunocompromised patients. This study elucidates the anti-inflammatory and barrier-protective effects of CEC15 against intestinal mucositis.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-Free Supernatant of Vaginal Viridans Streptococcus Induces Membrane Permeabilization and Transcriptional Regulation in Methicillin-Resistant Staphylococcus aureus.","authors":"Jake Adolf V Montecillo, Heon Jong Yoo, Yoo-Young Lee, Chulmin Park, Angela Cho, Hyunsu Lee, Nora Jee-Young Park, Hyung Soo Han, Gun Oh Chong, Incheol Seo","doi":"10.1007/s12602-024-10412-2","DOIUrl":"https://doi.org/10.1007/s12602-024-10412-2","url":null,"abstract":"<p><p>The genus Streptococcus is a heterogenous group of commensal and pathogenic bacteria that are normal inhabitants of the human body, including the female genital tract (FGT). In the FGT microbiome, streptococci represent two major groups: the pathogenic group B Streptococci (GBS) and the commensal viridans group streptococci (VGS). Though members of the VGS are frequently detected from the FGT, their role in the FGT microbiome remains underexplored. Here, we report the characterization of Streptococcus sp. K0074, isolated from the vaginal swab of an endometrial cancer patient admitted to the hospital, with no evidence of bacterial vaginosis. Phylogenetic analysis revealed that the strain is a member of the commensal VGS and possibly represents a novel species in the mitis subgroup. The strain demonstrated the production of low molecular weight bacteriocin-like substance with narrow-spectrum antagonistic activity, affecting the growth, biofilm formation, and colonization of aerobic vaginitis (AV)-causing bacterium methicillin-resistant Staphylococcus aureus (MRSA). The putative bacteriocin exhibited cell membrane-permeabilizing activity and exerted negative regulatory effect on the accessory gene regulator and SaeRS two-component systems of MRSA. Collectively, our results suggest that the isolate may modulate the FGT microbiome by inhibiting or displacing specific pathogen. Furthermore, the results presented here highlight new perspectives regarding the existence of VGS in the FGT microbiome and in particular pinpoint the potential clinical significance of the isolated VGS strain Streptococcus sp. K0074 for the treatment of AV caused by MRSA.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the Probiotic Supplement Landscape: Market Offerings, Sales Patterns, and Future Forecasts Using Machine Learning Approach - A Case Study of Montenegro.","authors":"Martinović Anđela, Martinović Ivan, Diego Mora, Stefania Arioli","doi":"10.1007/s12602-024-10400-6","DOIUrl":"https://doi.org/10.1007/s12602-024-10400-6","url":null,"abstract":"<p><p>Global increasing awareness about the health benefits of probiotics resulted to explorational growth in probiotic food supplement market. However, in some countries such as Montenegro, specific probiotic supplement regulation and comprehensive market analysis are absent, hampering the understanding of consumer preferences, market trends, and potential economic impacts of this industry. This article aims to delve into the Montenegrin market of probiotic food supplements, thoroughly examining various product types and their key characteristics. Using the case study of a pharmacy chain, as an example of organizational level, the sales, sale patterns, and trends are examined. Furthermore, we developed and employed a machine learning model for forecasting future sales. The market analysis highlighted the importance of setting national probiotic supplement regulations to enhance Montenegrin consumer understanding and trust, ensuring product efficacy and safety. Our study clearly showed increased interest in probiotic supplements as well as a constant positive trend in probiotic supplement sales. Furthermore, we found the correlation between foreign tourist visits in Montenegro and the yearly seasonality of probiotic supplement sales. Developed support vector regression machine learning model on time series data showed a good forecasting accuracy, clearly indicating that the same could be used for national sales forecasting. The insights from this study could promote the establishment of national probiotic supplement regulations, enhancing consumer protection and market credibility. Additionally, developed machine learning model provides the industry with valuable predictive tool, enabling companies to optimize their supply chains, effectively meet demand, and make data-driven decisions that could support sustainable market growth.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Heat-Killed Probiotic Strains on Biofilm Formation, Transcription of Virulence-Associated Genes, and Prevention of UTIs in Mice.","authors":"Yueh-Ying Chen, Zhen-Shu Liu, Bo-Yuan Chen, Hon-Man-Herman Tam, Wei-Yau Shia, Hsin-Hsuan Yu, Po-Wen Chen","doi":"10.1007/s12602-024-10399-w","DOIUrl":"https://doi.org/10.1007/s12602-024-10399-w","url":null,"abstract":"<p><p>Urinary tract infections (UTIs) pose a substantial healthcare challenge, exacerbated by the biofilm-forming abilities and antibiotic resistance of uropathogens. This study investigated the inhibition of biofilm formation (anti-biofilm) and dispersion of pre-established biofilm properties of 18 heat-killed probiotics and their supernatants against four antibiotic-resistant uropathogens: UPEC, Klebsiella pneumoniae (KP), Methicillin-resistant Escherichia coli (MREC), and Methicillin-resistant Staphylococcus pseudintermedius (MRSP). Supernatants from 14 probiotic strains significantly (P < 0.001) inhibited UPEC biofilm formation, reducing it by 20-80%, and also showed promise in removing existing biofilms by 10-60% (P < 0.001). Eight strains significantly (P < 0.05 to < 0.001) inhibited MREC biofilm formation, with four strains achieving 50-80% dispersion. Seventeen strains of heat-killed probiotics directly inhibited UPEC biofilm formation by 10-60% (P < 0.05 to < 0.001), but were less effective against MREC and MRSP (10-50% reduction; P < 0.05 to < 0.001) and had limited impact on KP (10% reduction; P < 0.05 to < 0.001). Notably, heat-killed probiotic like LGA, LGC, LGD, TP-8, and TP-4 showed the most significant inhibitory and dispersion of biofilm activity. RT-qPCR analysis further revealed these inactivated probiotics downregulated genes associated with pili and biofilm formation (fimA, csgA) and upregulated genes linked to quorum sensing (luxS, qseBC, sdiA). Therefore, these findings suggest that paraprobiotic treatment could inhibit the formation of pili and biofilms and promote biofilm dispersion. In an animal model, mice given paraprobiotic formulations I (16 strains) and II (a specific mixture) for 2 weeks showed reduced urinary bacterial load (P < 0.05). Paraprobiotic I notably reduced morbidity from bacteriuria (> 10⁵ CFU/ml) by 5 to 30% within the first 5 days post-infection compared to placebo. These findings highlight the potential of specific heat-killed probiotics in combating biofilms and preventing UTIs.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement and Recovery of Intestinal Flora Disorder Caused by Ciprofloxacin Using Lactic Acid Bacteria.","authors":"Xiumin Su, Li Su, Mengyuan Cao, Yulu Sun, Jinghan Dai, Yuanjie He, Wei Li, Wupeng Ge, Xin Lv, Qiang Zhang, Shenghui Cui, Jia Chen, Baowei Yang","doi":"10.1007/s12602-024-10401-5","DOIUrl":"https://doi.org/10.1007/s12602-024-10401-5","url":null,"abstract":"<p><p>In this study, four lactic acid bacteria (LAB) strains demonstrating ciprofloxacin, bile salt, gastric fluid, and intestinal fluid tolerance as well as adhesion ability to Caco-2 and HT-29 cells were used to improve and recover the intestinal flora disorders caused by ciprofloxacin, among which, Lactobacillus brevis 505 exhibited excellent adhesion ability to two kinds of cells and colonization ability to mouse intestinal. After ciprofloxacin treatment, certain recovery effect on cecum caused by ciprofloxacin in the mice was found during natural recovery (group 5C2), but it was challenging to fully restore the intestinal integrity to the initial level. After L. brevis 505 intervention (group 5C5), the intestinal damage to the colon and ileum caused by ciprofloxacin in mice was significantly alleviated; the recovery effect was better than that of natural recovery. Additionally, L. brevis 505 could effectively regulate INF-γ, sIgA, and RegIIIγ increase induced by ciprofloxacin. Shannon and Simpson index of the intestinal flora of mice in 5C5 group were higher than those in other group, the relative abundance of Bifidobacterium and Lactobacillus in the mice in 5C5 group was increased, indicating that LAB can better restore the structure and abundance of intestinal microflora. Consequently, L. brevis 505 shows promise as a probiotic for gut microbiota restoration and rebuilding during antibiotic therapy.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}