Probiotics and Antimicrobial Proteins最新文献

{"title":"Exploring the Impact of Selected Functional Feed Additives on Growth Performance, Nutrient Utilization, Enzyme Activities and Immune Gene Expression of Striped Murrel (Channa striata) Juveniles.","authors":"Govindharaj Sathishkumar, Nathan Felix, Amit Ranjan, Arumugam Uma, Kalaivanan Rajalakshmi","doi":"10.1007/s12602-025-10598-z","DOIUrl":"https://doi.org/10.1007/s12602-025-10598-z","url":null,"abstract":"<p><p>Incorporating selected functional feed additives such as β-glucan, mannan oligosaccharides (MOS), Saccharomyces cerevisiae and Lactobacillus acidophilus into fish diets enhances immunity, gut health, growth and stress tolerance. These natural supplements offer sustainable alternatives to antibiotics, addressing disease outbreaks and poor feed efficiency common in intensive aquaculture. Their use supports eco-friendly practices while improving fish performance and overall farm productivity. The impacts of incorporating varied nutritional supplements into the diet, including β-glucan, MOS, yeast (Saccharomyces cerevisiae) and Lactobacillus acidophilus on growth performance, nutrient utilization, digestive and antioxidant enzyme activities, hemato-biochemical profile and immune gene expression in striped murrel (Channa striata) were examined. Five experimental diets were formulated and prepared as follows: Control, D1 (0.2% β-glucan), D2 (0.3% MOS), D3 (1% Saccharomyces cerevisiae) and D4 (0.02% L. acidophilus). A total of 450 striped murrel (average weight: 15.12 ± 0.26 g per fish) were randomly assigned to 15 experimental cages (1 m × 1 m × 1.5 m; 30 fish per cage) and fed the respective diets three times a day (08.00, 13.00 and 18.00 h) to apparent satiety for 60 days. The findings of the experimental feeding trial elucidated that significantly higher (p < 0.05) growth performance and nutrient utilization were observed in fish fed with the D4 diet, compared to other diets. The dietary supplementation of different probiotic and prebiotic supplemented diets did not significantly affect the whole-body proximate composition and antioxidant enzyme activity in striped murrel. However, fish fed the D4 diet showed significantly higher (p < 0.05) digestive enzyme activity and immune gene (TGF-β1 and NF-κB) expression, compared to other diets. In conclusion, dietary supplementation with 0.02% L. acidophilus (D4 diet) resulted in improved growth performance, digestive enzyme activities and immune gene expression in striped murrel (C. striata).</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum WB4201, WB4202, and WB4203 Modulated Adipogenesis, Lipogenesis, and Fatty Acid β-oxidation in 3T3-L1 Cells.","authors":"Su Yeon Song, Jong-Woo Kim, Na-Kyoung Lee, Hyun-Dong Paik","doi":"10.1007/s12602-025-10587-2","DOIUrl":"https://doi.org/10.1007/s12602-025-10587-2","url":null,"abstract":"<p><p>Obesity is a growing public health concern characterized by excessive lipid accumulation and metabolic dysfunction. In this study, Lactiplantibacillus plantarum WB4201, WB4202, and WB4203 were isolated from kimchi and evaluated for their probiotic properties and anti-obesity effects. These strains exhibited strong probiotic potential, demonstrating high gastric tolerance, intestinal adhesion, and safety profiles. Notably, heat-treated L. plantarum strains significantly reduced triglyceride content and inhibited lipid accumulation by modulating metabolic pathways involved in adipogenesis, lipogenesis, and fatty acid β-oxidation. The heat-treated L. plantarum strains downregulated key regulators of adipocyte differentiation, including CCAAT/enhancer-binding protein α, peroxisome proliferator-activated receptor γ, and RAC-β serine/threonine-protein kinase. In addition, they suppressed lipid synthesis by downregulating the expression of fatty acid synthase, sterol regulatory element-binding protein 1c, glycerol-3-phosphate acyltransferase, and acetyl-CoA carboxylase 1. Furthermore, they promoted fatty acid oxidation by increasing the expression of carnitine palmitoyltransferase 1 while reducing cluster of differentiation 36. These findings demonstrate that heat-treated L. plantarum WB4201, WB4202, and WB4203 can effectively modulate lipid metabolism and may serve as novel ingredients for the development of functional foods targeting obesity prevention and metabolic health improvement.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Probiotics: Exploring the Role of the Gut Microbiome in Combating Obesity.","authors":"Riyasdeen Anvarbatcha, Faisal Kunnathodi, Amr A Arafat, Sarfuddin Azmi, Mohammad Mustafa, Ishtiaque Ahmad, Haifa F Alotaibi","doi":"10.1007/s12602-025-10605-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10605-3","url":null,"abstract":"<p><p>Obesity has become a global health crisis driven by genetic, environmental, and lifestyle factors, often linked to gut microbiome imbalances. Probiotics, particularly Lactobacillus and Bifidobacterium strains, have shown promise in clinical trials by promoting weight loss, improving lipid profiles, and addressing gut dysbiosis associated with obesity. This review surveys the literature on the microbiome and obesity, emphasizing the clinical relevance of probiotics in treatment strategies. Our comprehensive PubMed search highlights the mechanisms through which probiotics influence metabolic health, including their effects on inflammation and appetite regulation. We also explore promising future research directions and the potential for integrating probiotics into clinical practice. While results are encouraging, the evidence is limited by strain variability, small sample sizes, short trial durations, and individual differences in microbiota composition. More extensive, long-term studies with standardized methods are crucial to confirm the effectiveness of probiotics as viable anti-obesity treatments.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-Driven Visualization of the Dynamics of Antimicrobial Peptides in Cell Death.","authors":"Dan Hou, Jiatong Zhao, Mingshi Guo, Xinran Zhang, Shuiqing Yu, Jiayue Li, Tymour Forouzanfar, Qing Zhang, Janak L Pathak","doi":"10.1007/s12602-025-10578-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10578-3","url":null,"abstract":"<p><p>This study explores the current status, research hotspots, and emerging trends in AMP-induced cell death through bibliometric and data-driven visual analysis. The findings aim to provide researchers and clinical professionals with new insights and potential research directions. A total of 1,897 articles and reviews published between 2006 and 2024 were retrieved from the Web of Science Core Collection. Bibliometric and visual analyses were conducted using CiteSpace, VOSviewer, Scimago Graphica, Origin 2022, and WordClouds. The analysis focused on publication trends, contributing institutions, journals, authors, cited references, and keywords. China contributed the largest share of publications (28.15%). The Chinese Academy of Sciences emerged as the most collaborative institution, demonstrating the highest centrality. The author with the highest composite index was Chen, Jyh-Yih (2,985.27). Recent research hotspots have centered on elucidating the mechanisms of AMP-induced cell death and exploring the potential applications of AMPs in cancer therapy. Keywords such as anticancer peptides, mechanism, design, and antibiotic resistance currently dominate the field, reflecting its evolving focus. Research on the application of AMPs in cancer treatment is gaining momentum. The forefront of this field involves modifying and designing AMPs to address antibiotic-resistant bacterial infections and advance cancer therapeutics. However, further investigation is needed to uncover the specific molecular mechanisms underlying AMP-induced cell death, including necrosis, pyroptosis, and ferroptosis.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiome Interventions: From Dysbiosis to Next-Generation Probiotics (NGPs) for Disease Management.","authors":"Mandeep Kumar Gupta, Rajnish Srivastava","doi":"10.1007/s12602-025-10582-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10582-7","url":null,"abstract":"<p><p>The gut microbiome, sometimes referred to as the \"second brain,\" the \"lost organ,\" the \"identification card of the individual,\" and the \"fingerprint of the host,\" possesses diverse traits and functions that influence health. The impact of gut commensal bacteria on health, as opposed to environmental pathogenic factors, has generated increasing interest in recent years, culminating in a substantial body of study. Research indicates that dysbiosis of the intestinal microbiota is commonly observed in chronic inflammatory diseases, including colitis, obesity/metabolic syndrome, diabetes mellitus, liver infections, allergic conditions, cardiovascular diseases, COVID-19, cancers, and neurodegenerative disorders. The International Scientific Association for Probiotics and Prebiotics has recently refined the theory of complementary and synergistic synbiotics. In recent years, the field of microbiome research has been significantly advanced by technological developments such as massive culturomics, gnotobiotics, metabolomics, parallel DNA sequencing, and RNA sequencing. This review article examined the potential next generation probiotics (NGPs) and explored some of them, Faecalibacterium prausnitzii, Bacteroides thetaiotaomicron, Akkermansia muciniphila, Parabacteroides goldsteinii, Bacteroides fragilis, Eubacterium hallii, Roseburia intestinalis, Christensenella minuta, Prevotella copri, and Oscillospira guilliermondii. In addition to these useful probiotic strains, psychobiotics, members of the families of Lactobacilli, Streptococci, Bifidobacteria, Escherichia, and Enterococci, have extended applicability in the use for neurodevelopmental and neurodegenerative disorders. The article also reviewed current trends and limitations in NGPs to enhance our comprehensive understanding of key concepts associated with the consumption of probiotics and proposed necessary initiatives for researchers to engage in collaborative translational research as future therapeutic solutions.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Modes of Action of a Recombinant Antimicrobial Peptide, Hepcidin (rGf-Hep), from Gerres filamentosus Against Pathogenic Vibrios: Membrane Disintegration and Reactive Oxygen Species Generation Leading to Cell Death.","authors":"Anooja V V, Anju M V, Athira P P, Archana K, Neelima S, Muhammed Musthafa S, Dhanya Kesavan, Rosamma Philip","doi":"10.1007/s12602-025-10591-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10591-6","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are essential components of the innate immune response, which play a significant role in combating pathogenic infections. Hepcidin, a peptide hormone predominantly synthesized in the liver, has been identified to exhibit dual functions in iron metabolism and antimicrobial activity across various organisms. In this study, we describe the molecular characteristics, anti-vibrio activity, and mechanisms of action of a novel hepcidin isoform from the commercially important estuarine fish, Whipfin silver-biddy (Gerres filamentosus). The open reading frame of hepcidin cDNA sequence was 273 base pairs in length, encoding a peptide of 90 amino acids. The active region Gf-Hep contains eight well-conserved cysteine residues which form disulfide bridges stabilizing the antiparallel beta sheet conformation of the peptide. Featuring a C-terminal furin cleavage site (RXXR) within the prodomain and an N-terminal 'QSHI/LS' motif in the mature region, Gf-Hep is classified with the HAMP1 group of fish hepcidins. Recombinantly expressed Gf-Hep exhibited robust antimicrobial activity against Vibrio parahaemolyticus, Vibrio fluvialis, Vibrio cholerae, and Vibrio alginolyticus. The modes of action of rGf-Hep included membrane depolarization, membrane permeabilization, and ROS production. With its potent antibacterial properties, direct killing mechanisms, and non-cytotoxic effects on normal cells, rGf-Hep holds promise to be developed as an effective anti-vibrio agent for aquaculture applications.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune-Enhancing Effects of Two Potential Probiotic Strains Latilactobacillus curvatus WiKim0169 and Pediococcus acidilactici WiKim0170 in a Cyclophosphamide-Induced Immunosuppression Rat Model.","authors":"Eun-Jung Park, You-Suk Lee, Eun-Min Jun, Byung Wook Lee, Sang Min Park, Hae-Jeung Lee","doi":"10.1007/s12602-025-10585-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10585-4","url":null,"abstract":"<p><p>Emerging evidence suggests that probiotics are beneficial for immunity and play a crucial role in regulating gut microbiota. However, the immune-enhancing effects of specific bacterial species remain uncertain. This study investigated the effects of two potential probiotic strains, Latilactobacillus curvatus WiKim0169 (Wilac L004, W4) and Pediococcus acidilactici WiKim0170 (Wilac L002, W2) isolated from fermented cabbage, on immune enhancement and gut microbiota changes in a cyclophosphamide-induced immunosuppression rat model. The results revealed that W4 and W2 improved natural killer cell activity, serum nitrite, and immunoglobulin levels. They also increased cytokine levels and activated the nuclear factor-κB pathway, substantiating the underlying mechanism of the immune-enhancing effects. Additionally, both strains altered gut microbiota composition by increasing bacteria that are being studied for their potential association with immune function. Taken together, both strains improved immune-related biomarkers and the gut microbiota. These findings suggest W4 and W2 as promising probiotics with immune-enhancing properties.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating Cadmium Toxicity: The Therapeutic Potential of Lactobacillus acidophilus Pro4 in Mice.","authors":"Ahmed M Darwish, Abd El-Nasser A Khattab, Abdulrahman A Almehizia, Hamad M Alkahtani, Hazem A Ghabbour, Atef Kalmouch","doi":"10.1007/s12602-025-10581-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10581-8","url":null,"abstract":"<p><p>Cadmium (Cd) is a highly toxic heavy metal known to negatively impact multiple bodily systems. This study investigated the effectiveness of Lactobacillus acidophilus Pro4, a probiotic strain isolated from natural pickles, as an anti-cadmium toxicity supplement. Forty male albino mice were divided into five groups: the control group (C), the probiotic group (Pro4), the cadmium group (Cd), the protective group (Pr), and the therapy group (Th). The body weight, liver functions, and tissue pathology of liver and the expression of IGF1, GH, GH1, and GHR1 genes were assessed. Cadmium exposure significantly impacted the mice, reducing their daily weight gain and causing liver damage. This was evident by elevated levels of GOT and GPT enzymes in the serum, along with signs of degeneration in the liver tissue, and downregulation of the expression of IGF1, GH, GH1, and GHR1 genes compared to the control group. Mice in the Pro4 group displayed increased daily weight gain and the expression of IGF1, GH, GH1, and GHR1 genes compared to other groups. While GOT levels decreased significantly compared to the control group, GPT levels remained unchanged. Liver tissue appeared mostly normal, with a slightly dilated central vein and some inflammatory cells. In the Th group, the results were encouraging but not a complete reversal of damage. Levels of GOT and GPT enzymes improved compared to the cadmium-only group, and the liver tissue showed signs of repair. Therefore, the novel Lactobacillus acidophilus Pro4 offers a promising approach to mitigate the adverse effects of cadmium on growth in mice by improving weight gain and regulating biochemical markers.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synbiotic Supplementation Mitigates Antibiotic-Associated Diarrhea by Enhancing Gut Microbiota Composition and Intestinal Barrier Function in a Canine Model.","authors":"Haoyi Shen, Yiran Zhao, Sizhe Zhang, Jiahui Xu, Jinjin Tong, Hua Yao, Hua Zhang","doi":"10.1007/s12602-025-10584-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10584-5","url":null,"abstract":"<p><p>Antibiotic-associated diarrhea (AAD) remains a significant clinical challenge, with current treatments often inadequate for fully restoring gut health. This study aimed to evaluate the effectiveness of synbiotics, including chitosan oligosaccharides, Bifidobacterium, Clostridium butyricum, and Lactiplantibacillus plantarum, in mitigating AAD in a canine model. Sixteen Beagle dogs were randomly assigned to an AAD model group or a synbiotic treatment group. Synbiotic supplementation significantly reduced diarrhea severity, improved intestinal morphology, and enhanced gut microbiota diversity. Furthermore, synbiotics upregulated the expression of tight junction proteins (ZO-1, occludin, claudin-1), thereby reducing mucosal permeability. A significant decrease in proinflammatory cytokines (IL-1β, TNF-α) further underscored the anti-inflammatory effects of the treatment. These findings suggest that synbiotics may offer a promising approach to restoring gut barrier integrity and modulating immune responses in cases of AAD. Further research is warranted to investigate the long-term impacts and underlying mechanisms of synbiotics in gut health restoration.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A Novel Synbiotic Protects Against DSS-Induced Colitis in Mice via Anti-inflammatory and Microbiota-Balancing Properties.","authors":"Yong Yang, Yuyu Qiao, Ge Liu, Weihao Chen, Ting Zhang, Jing Liu, Weiping Fan, Mingwei Tong","doi":"10.1007/s12602-025-10583-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10583-6","url":null,"abstract":"","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}