Antibiofilm Activity of the Marine Probiotic Bacillus subtilis C3 Against the Aquaculture-Relevant Pathogen Vibrio harveyi.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2025-06-01 Epub Date: 2024-02-08 DOI:10.1007/s12602-024-10229-z

Coraline Petit, Flore Caudal, Laure Taupin, Alain Dufour, Carine Le Ker, Fanny Giudicelli, Sophie Rodrigues, Alexis Bazire

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引用次数: 0

Abstract

V. harveyi is a well-known pathogen-inducing vibriosis, especially for shrimp, fish, and invertebrates. Its virulence is related to biofilm formation and this negatively impacts the aquaculture industry. Therapeutic strategies such as the utilization of probiotic bacteria may slow down Vibrio infections. In this study, we investigated the potential antibiofilm activity of the probiotic Bacillus subtilis C3 for aquaculture. First, B. subtilis C3 biofilm was characterized by confocal laser scanning microscopy (CLSM) before testing its bioactivities. We demonstrated antibiofilm activity of B. subtilis C3 culture supernatant, which is mainly composed-among other molecules-of lipopeptidic surfactants belonging to the surfactin family as identified by ultra-high-performance liquid chromatography (UHPLC)-MS/MS. Their antibiofilm activity was confirmed on V. harveyi ORM4 (pFD086) biofilm by CLSM. These findings suggest that the marine probiotic B. subtilis C3 might inhibit or reduce Vibrio colonization and thus decrease the associated animal mortalities.

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海洋益生菌枯草芽孢杆菌 C3 对水产养殖相关病原体 Harveyi 弧菌的抗生物膜活性

哈维氏弧菌（V. harveyi）是一种众所周知的致病性弧菌病，尤其是对虾、鱼类和无脊椎动物。它的毒性与生物膜的形成有关，这对水产养殖业造成了负面影响。利用益生菌等治疗策略可减缓弧菌感染。在这项研究中，我们调查了水产养殖中益生菌枯草芽孢杆菌 C3 的潜在抗生物膜活性。首先，在测试枯草芽孢杆菌 C3 的生物活性之前，我们用激光共聚焦扫描显微镜（CLSM）对其生物膜进行了表征。我们证明了枯草芽孢杆菌 C3 培养上清液的抗生物膜活性，经超高压液相色谱（UHPLC）-质谱/质谱（MS/MS）鉴定，其主要成分是属于表面活性剂家族的脂肽类表面活性剂。它们的抗生物膜活性通过 CLSM 在 V. harveyi ORM4 (pFD086) 生物膜上得到了证实。这些研究结果表明，海洋益生菌枯草杆菌 C3 可抑制或减少弧菌的定植，从而降低相关动物的死亡率。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.