Probiotics and Antimicrobial Proteins最新文献

{"title":"Promising Antidepressant Potential: The Role of Lactobacillus rhamnosus GG in Mental Health and Stress Response.","authors":"Musab Işık, Fadime Köse, Cansu Özbayer, Özcan Budak, Rumeysa Keleş Kaya, Derya Güzel Erdoğan, Mehmet Arif Demirci, Songül Doğanay, Cahit Bağcı","doi":"10.1007/s12602-025-10470-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10470-0","url":null,"abstract":"<p><p>Chronic stress is linked to changes in brain physiology and functioning, affects the central nervous system (CNS), and causes psychiatric diseases such as depression and anxiety. In this study, antidepressant effects of the probiotic bacterium Lactobacillus rhamnosus GG (ATCC 53103) (LGG) (15 × 10⁸ cfu/ml/day) on the mechanisms playing a role in the pathophysiology of depression were investigated, and the results were compared with the effects of bupropion (20 mg/kg/day) and venlafaxine (20 mg/kg/day). A total of 56 male Wistar Albino rats were used in control, stress, bupropion, venlafaxine, LGG, bupropion + stress, venlafaxine + stress, LGG + stress groups, n = 7 each. Changes in the body weight of the rats during the experiment were determined by weight measurement. Gene expression levels were determined by the RT-PCR method. Four different behavioral tests were performed to evaluate depressive behaviors (sucrose preference test, three-chamber sociability test (social interaction test), elevated plus maze test, forced swim test). LGG treatment was effective in reducing depressive-like behaviors, increased BDNF level, 5-HT1A, DRD1, ADRA-2A, GABA-A α1, CNR1 expression levels in the hippocampus and NOD1 receptor expression level in the small intestine (p < 0.05), and also decreased neurodegeneration level, glial cell activity, and intestinal permeability in depressed rats. As a result, it was revealed in this study for the first time that the LGG probiotic bacterium has antidepressant properties and was found to be more effective than the antidepressant drugs bupropion and venlafaxine. Our results suggest that LGG is a potential psychobiotic bacterium and can be useful to treat depression. It may be an effective and useful option in combating depression.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immuno-oncological Challenges and Chemoresistance in Veterinary Medicine: Probiotics as a New Strategic Tool.","authors":"Muhammad Farrukh Nisar, Tingdong Yan, Yi Cai, Chunpeng Wan","doi":"10.1007/s12602-025-10468-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10468-8","url":null,"abstract":"<p><p>Cancer has the highest death rates due to increased immuno-oncological (IO) challenges and chemoresistance caused by gut dysbiosis, whereas administration of probiotics may reverse these responses against anticancer therapies. Recently, immunotherapeutics have extensively been focused for significant advancements in pharmacological drug discovery and clinical outcomes. Mammals have intestinal epithelial cells, mucosal immune cells, and indigenous gut microbiota which may reshape immunotherapeutics efficacy. These include use of T-cell immune checkpoint inhibitors (ICPI), genetically engineered T-cells, tumor vaccines, monoclonal antibodies (mAbs), and anti-B- and T-cell antibodies. Immunotherapeutics for cancer treatment became popular in both veterinary and human health care systems due to their strong inhibitory actions against PD-1 and CTLA-4 to check tumorigenesis. IO issues in animals also need special attention, where caninized mAbs targeting CD-20 and CD-52 have been clinically used in treating canine B-cell and T-cell lymphomas, respectively. Probiotics appeared as strong immunotherapeutics that might be shaping the epigenetics of the organisms specifically in animal breeding practices for desired features, but limited literature regarding the immunomodulatory effects in humans and animals is available. In addition, considering the important role of probiotics in humans and veterinary medicine, a new perspective on the probiotic-mediated modulation of ncRNAs (miRNAs, lncRNAs, circRNAs) is also highlighted and would be a new therapeutic tool. This review provides insight into the cellular processes and pharmacological activities for treating veterinary infectious diseases and covers small drug molecules as ncRNA-modulators in veterinary medicine.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics: A New Approach for the Prevention and Treatment of Cervical Cancer.","authors":"Hui Li, Zhen Xu, Chaoqi Liu, Jinfang Deng, Ziqin Li, Yongqin Zhou, Zhiying Li","doi":"10.1007/s12602-025-10479-5","DOIUrl":"https://doi.org/10.1007/s12602-025-10479-5","url":null,"abstract":"<p><p>Cervical cancer is closely linked to high-risk human papillomavirus (HR-HPV) infections which could crosstalk with the diversity of microorganisms within the cervicovaginal microbiota (CVM). Conventional treatments can disrupt the host microbial communities, which in turn affect the efficacy and side effects of these interventions, potentially jeopardizing the lives and health of patients. Therefore, there is a need to develop new approaches for the prevention and treatment of cervical cancer, and probiotics have shown significant potential as effective agents against cervical cancer. Studies have demonstrated that probiotics induce anticancer action by inducing apoptosis in tumor cells, inhibiting metastasis, reducing inflammation, enhancing immune functions, and modifying the effect of chemo/radiation therapy. Recent reports showed that probiotics had the ability to restore the CVM, clear HPV infections in cervical epithelial cells, and modulate immune responses. Despite these promising findings, the efficacy and safety of probiotic formulations require further validation through comprehensive research. This review focused on elucidating the mechanism behind the anticancer action and the importance of addressing the challenges associated with the clinical application of probiotics to realize their full potential as a novel and complementary approach in the prevention and treatment of cervical cancer.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum ZJ316 Alleviates Helicobacter pylori-Induced Intestinal Inflammation by Sustaining Intestinal Homeostasis.","authors":"Shiying Wu, Fangtong Wei, Yongqiang Chen, Ziqi Chen, Yuenuo Luo, Jiayi Fan, Yang Xu, Mingyang Hu, Ping Li, Qing Gu","doi":"10.1007/s12602-025-10474-w","DOIUrl":"https://doi.org/10.1007/s12602-025-10474-w","url":null,"abstract":"<p><p>Helicobacter pylori (H. pylori) infection poses significant risks for gastric cancer and intestinal inflammation, yet effective prevention strategies for intestinal inflammation remain elusive. Here, we aimed to investigate the protective effects and underlying mechanisms of Lactiplantibacillus plantarum ZJ316 (L. plantarum ZJ316) in a mouse model of H. pylori-induced intestinal inflammation. The reverse transcription‑quantitative polymerase chain reaction (RT-qPCR) analysis showed that treatment with L. plantarum ZJ316 significantly upregulated the mRNA levels of tight junction proteins, including Zonula occludens-1 (ZO-1), Occludin, and Claudin-1, while simultaneously decreasing the expression levels of pro-inflammatory cytokines interleukin-1β (IL-1β), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α). Additionally, 16S rDNA sequencing analysis revealed that administration of L. plantarum ZJ316 reduced relative abundance of pathogenic bacteria, Staphylococcus and Desulfovibrio by 69% and 42%, respectively, while enhancing beneficial bacteria including Ligilactobacillus, Akkermansia, and Lactobacillus associated with short-chain fatty acids (SCFAs) synthesis by 88%, 85%, and 16%, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis confirmed L. plantarum ZJ316 restored SCFA levels reduced by H. pylori infection. In vitro, L. plantarum ZJ316 inhibited the IκBα/NF-κB pathway, thereby reducing TNF-α and IL-8 production in HT-29 cells following H. pylori infection. These findings collectively suggest that L. plantarum ZJ316 ameliorates H. pylori-induced intestinal inflammation by enhancing gut barrier function, improving flora structure, increasing SCFA levels, and mitigating inflammation through NF-κB pathway inhibition, offering promise for therapeutic development.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Type I Crustin Isoform from Scylla olivacea and its Antibacterial Potential: Membrane Depolarization, Disruption, and Induction of Reactive Oxygen Species as Modes of Action.","authors":"S Neelima, M V Anju, K Archana, V V Anooja, P P Athira, M R Revathy, M Dhaneesha, A Muneer, T P Sajeevan, S Muhammed Musthafa, I S Bright Singh, S Muraleedharan Nair, Rosamma Philip","doi":"10.1007/s12602-025-10469-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10469-7","url":null,"abstract":"<p><p>Availability of novel antimicrobial agents is an urgent necessity to combat the growing threat posed by multidrug-resistant bacteria, prompting exploration of marine antimicrobial peptides (AMPs) as potential solutions. Crustacean AMPs are equitably diverse in terms of structure and function, making them consistent templates for novel antimicrobials. From Scylla olivacea gill transcriptome cDNA, a putative Crustin AMP sequence of 333 nucleotides, encoding a 111 amino acid Crustin Type-I isoform was identified. The mature peptide encoding region was cloned and recombinantly expressed in E. coli using Luria Bertani (LB) broth, yielding approximately 0.9 mg/L peptide. This cationic (+6.25) peptide with amphipathic properties (34% hydrophobicity) exhibited antibacterial effects against Gram-positive and Gram-negative strains, with MIC 16 μM against Vibrio spp. The identified modes of actions included disruption of bacterial membranes, membrane potential dissipation, and induction of ROS. Scanning electron microscopy (SEM) analysis revealed bacterial lysis and structural damage. Being non-toxic to mammalian cells (CHO-K1) and non-haemolytic, So-Crustin qualifies to be safe for therapeutic applications. It was quite stable under different physio-chemical/biological conditions, including temperature, pH, NaCl concentrations and proteases like trypsin and proteinase K. This study emphasizes So-Crustin's potential as a safe and effective antibacterial agent.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Bioactive Compounds Enriched Functional Yogurt: Impact on the Probiotic Bacteria and Its Potential Health Benefits.","authors":"Moumika Rahman Brishti, Gopinath Venkatraman, Ahmad Salihin Bin Hj Baba, Noor Liana Mat Yajit, Saiful Anuar Karsani","doi":"10.1007/s12602-025-10461-1","DOIUrl":"https://doi.org/10.1007/s12602-025-10461-1","url":null,"abstract":"<p><p>Recently, there has been a high demand for the development of yogurt-based nutraceuticals and functional medications. This surge is primarily driven by the increasing global need for pharmaceutical and nutraceutical products, arising from widespread nutrient deficiencies and the emergence of various communicable and non-communicable diseases (NCDs), including respiratory infections, cancer, gastrointestinal, diabetes, obesity, and cardiovascular diseases. Probiotic yogurt provides an effective medium for delivering essential nutrients to the human body. Additionally, various prebiotic combinations, such as bioactive compounds from plants, animals, and microbes, can enrich the viability of probiotics, nutritional value, and efficacy. However, the gastric environment can significantly impact the viability of probiotic microorganisms as well as the absorption of nutrients and bioactive molecules. Therefore, utilizing biopolymer-based encapsulation for functional nutrients, metal nanostructures, and medications can improve the bioavailability of these compounds, protect the probiotics from gastric enzymes, increase nutrient and microbial absorption in colonic fluids, and enhance the antioxidant level in the body. This review investigates various methods for producing yogurt enriched with prebiotic and probiotic combinations alongside techniques such as microencapsulation, emulsification, and the incorporation of metal nanoparticles. Key factors such as viability, texture, and syneresis are examined to optimize yogurt-based nutraceuticals and functional medications.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus delbrueckii Alleviate Oxidative Stress and Intestinal Injuries by Activating TLR2 and TLR4 Expressions in IPEC-J2 Cells.","authors":"Fengming Chen, Shu Wu, Xiaoyu Wang, Yinghui Li, Binsheng He, Yafen Fu, Xingguo Huang, Jiayi Chen","doi":"10.1007/s12602-025-10466-w","DOIUrl":"https://doi.org/10.1007/s12602-025-10466-w","url":null,"abstract":"<p><p>Reducing the negative impact of oxidative stress (OS) on weaned piglets is crucial for the further development of the pig industry. Our previous studies found that Lactobacillus delbrueckii (LAB) can alleviate OS and protect intestinal health by activating TLR2 and TLR4 expressions in weaned piglets. This research aimed to verify whether LAB can alleviate OS and intestinal barrier injuries in IPEC-J2 cells by activating TLR2 and TLR4. IPEC-J2 cells were first pretreated with 10¹⁰ CFU/mL LAB for 12 h, and then were induced to OS by 0.8 mmol/L H₂O₂ for another 12 h. The results showed that LAB significantly alleviated OS induced by H₂O₂, indicated by the downregulation of MDA (P < 0.05), along with upregulation of GSH-Px, CAT, T-SOD, and T-AOC (P < 0.05). Besides, LAB significantly alleviated intestinal barrier injuries induced by H₂O₂, indicated by the upregulation of Claudin-1, Occludin, and ZO-1 expressions. Subsequently, the IPEC-J2 cells were pretreated with inhibitors of TLR2 (TLR2-IN-C29) and TLR4 (MD2-TLR4-IN-1) in the absence of LAB for 12 h, and then treated with 0.8 mmol/L H₂O₂ for another 12 h. The results showed that both inhibitors significantly prevented the protection effects of LAB on H₂O₂-induced OS, indicated by the downregulation of CAT, SOD1, HO-1, and GSH-Px1 expressions (P < 0.05). Taken together, LAB treatment can alleviate OS and intestinal injuries induced by H₂O₂ by activating TLR2 and TLR4 expressions in IPEC-J2 cells, thus providing novel theoretical support for LAB in the prevention and treatment of oxidative damage.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory Effects of Multi-species/Multi-strain Synbiotic and L-carnitine Concomitant Supplementation on Atherogenic-Indices, Body Composition, Visceral Obesity, and Appetite in Metabolically Healthy Women with Obesity: A Double-Blind Randomized Controlled Clinical Trial.","authors":"Farnoush Fallah, Reza Mahdavi","doi":"10.1007/s12602-025-10460-2","DOIUrl":"https://doi.org/10.1007/s12602-025-10460-2","url":null,"abstract":"<p><p>Obesity, a chronic disease with pandemic proportions, is recognized as a major risk factor for cardiometabolic disorders due to its association with atherogenic dyslipidemia, a common characteristic attributed to visceral adiposity in patients with obesity. Atherogenic and visceral-obesity indices have been conceded as surrogate cardiovascular diseases (CVD) indicators surpassing the conventional markers due to stronger predictive power for obesity-induced cardiometabolic risk and CVD mortality rate. Nutraceuticals have been suggested as emerging approaches to counteract obesity-associated cardiometabolic disorders. Considering the evidence addressing the ameliorating effects of either L-carnitine or biotics on metabolic indices, also the reports addressing higher efficacy of concomitant supplementation versus single-therapies, this clinical trial was conducted to assess the effects of L-carnitine + multi-species/multi-strain synbiotic combined supplementation compared to L-carnitine mono-therapy on atherogenic-indices, body composition, visceral obesity, and appetite sensations in 46 metabolically healthy women with obesity, randomly assigned to co-supplementation (L-carnitine-tartrate (2 × 500 mg/dl) + synbiotic (one capsule/day)) or mono-therapy (L-carnitine-tartrate (2 × 500 mg/dl) + maltodextrin (one capsule/day)) groups for 8 weeks. L-carnitine + synbiotic co-supplementation led to a significantly greater reduction in atherogenic-indices including atherogenic-index-of-plasma (AIP), Castelli's-risk-index-I (CRI-I), Castelli's-risk-index-II (CRI-II), atherogenic-coefficient (AC), lipoprotein-combine index (LCI), systolic blood pressure (SBP), fat-mass (FM) weight/percent, visceral-adiposity index (VAI), waste-to-height ratio (WHtR), body-adiposity index (BAI), and appetite sensation scores compared to L-carnitine mono-therapy. L-carnitine + synbiotic combined supplementation was more efficient in improving atherogenic-indices as cardiovascular risk markers, body composition, visceral obesity, and appetite sensations in metabolically healthy women with obesity. Therefore, simultaneous supplementation of L-carnitine + synbiotic might be considered a promising approach to ameliorate cardiometabolic risk factors in healthy individuals with obesity. Further longer period studies are required to confirm these findings. (Iranian Registry of Clinical Trials (IRCT; https://irct.behdasht.gov.ir/trial/28048 ).</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms and Therapeutic Potential of Key Anti-inflammatory Metabiotics: Trans-Vaccenic Acid, Indole-3-Lactic Acid, Thiamine, and Butyric Acid.","authors":"Muñoz-Olivos Cristina, Bautista-Rodriguez Elizabeth, Rivas-Arreola María Jose, Palacios-Gonzalez Berenice, Zacapa Diego, Cortez-Sanchez Jose Luis","doi":"10.1007/s12602-025-10475-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10475-9","url":null,"abstract":"<p><p>Identifying metabolites produced by probiotic bacteria, also known as metabiotics, is becoming increasingly common due to their anti-inflammatory, anti-obesogenic, and immunomodulatory effects. Postbiotics alongside diet, regulate both physical and mental health, as the microbiota members can interact physically with host cells or through secretion of nutrients and metabiotics. These metabiotics also reduce the severity of certain metabolic disorders and support the proper functioning of various organs and systems. In this review, we describe the mechanisms of action of trans-vaccenic acid (TVA), indole-3-lactic acid (ILA), thiamine (vitamin B1), and butyric acid metabolites produced or induced by probiotics such as Lactobacillus and/or Bifidobacterium, among others and previously identified using analytical techniques such as mass spectrometry (LC-MS). Within their mechanisms of action, Trans-vaccenic acid exerts anti-inflammatory effects and helps alleviate complications associated with metabolic diseases. Indole metabolites promote IL-22 production and regulate epithelial cell proliferation and antimicrobial peptide production. Thiamin is essential for energy metabolism regulation, and butyric acid regulates the brain-gut axis and also regulates immune response. This review expands our understanding of the potential therapeutic use of metabiotics.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lacticaseibacillus rhamnosus: An Overview of the Viability in Fruit and Vegetable Juices and Their Potential Effects on Human Health.","authors":"Alessandra Peres Guimarães, Daniel Sgrancio Uliana, Marcella Ramos Sant'Ana, Jackline Freitas Brilhante de São José","doi":"10.1007/s12602-025-10454-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10454-0","url":null,"abstract":"<p><p>Consumer demand for health-enhancing foods is growing, with plant-based probiotic foods gaining prominence. Fruit and vegetable juices offer promising matrices for delivering probiotic strains due to their rich bioactive compound content, which holds nutritional and technological significance. Lacticaseibacillus rhamnosus is commonly employed in viability studies, offering both technological benefits and potential health advantages for consumers. The main databases were searched to bring together a compilation of studies that assessed the feasibility and technological parameters of fruit and vegetable juices enriched with Lacticaseibacillus rhamnosus. In addition, this review aims to analyze promising points, gaps, and challenges in the development of these products, such as the relationship between the technological aspect of manufacturing this type of food and impacts on human health. The strain most used in the studies was Lacticaseibacillus rhamnosus GG. More than half of the formulations maintained viability above 8 log CFU/mL. Low pH, high dissolved oxygen concentrations, and low free amino acid concentrations can all be obstacles to preserving viability in fruit and vegetable juices. Various techniques, including microencapsulation, prebiotic supplementation, and pH adjustments, have been employed by researchers to achieve more favorable results. Despite numerous meta-analyses on supplementation alone, human studies on the consumption of probiotic-enriched juices remain scarce, which highlight the need for more research.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}