Probiotics and Antimicrobial Proteins最新文献

{"title":"Biological Profiling of Postbiotics of Lactobacillus plantarum: Antibacterial, Antioxidant, and Cytotoxic Properties Under In Vitro and Food Circumstances.","authors":"Negin Hosseinzadeh, Amin Abbasi, Marjan Bazdar, Hossein Samadi Kafil, Vahideh Sarabi-Aghdam, Aziz Homayouni-Rad","doi":"10.1007/s12602-025-10752-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10752-7","url":null,"abstract":"<p><p>The objective of this study was to analyze the chemical profile (gas chromatography-mass spectrometry), cell-based safety (cell viability, SOD and GSH-Px activity, NO production, and ROS activity), antioxidant properties (hydroxyl-, DPPH-radical scavenging activity, and linoleic acid peroxidation inhibitory activity), biofilm and auto-aggregation inhibitory activity, and antibacterial effects (minimum inhibitory concentration, minimum bactericidal concentration and minimum effective concentration) of Lactobacillus plantarum postbiotics (LPPs) in relation to Escherichia coli and Staphylococcus aureus. LPPs demonstrated notable Radical scavenging activity, with values of 72.30 ± 3.41% for hydroxyl RSA, 95.76 ± 3.72% for DPPH, and 20.98 ± 1.67% for linoleic acid peroxidation inhibition. Additionally, they demonstrated antibacterial effects against S. aureus (29.45 mm inhibition zone, minimum inhibitory concentration (MIC) of 32 µg/mL, minimum bactericidal concentration (MBC) of 50 µg/mL, minimal effective concentration (MEC) of 30 mg/mL for whole milk and 55 mg/mL for meat) and E. coli (21.17 mm inhibition zone, MIC 80 µg/mL, MBC 120 µg/mL, MEC 65 mg/mL milk, 85 mg/mL meat) with statistical significance noted (P < 0.05). LPPs demonstrated a positive effect on KDR cell viability, resulting in a significant enhancement of SOD and GSH-Px functions in these cells. Additionally, LPPs resulted in a decrease in NO production and a reduction in ROS levels (P < 0.05). Consequently, LPPs present a promising strategy for addressing the proliferation of S. aureus and E. coli, and they hold potential for application in the food industry to mitigate safety concerns associated with pathogenic microbes.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synbiotic Effect of Saccharomyces boulardii and Fructans from Agave salmiana on the Modulation of Intestinal Microbiota in Children Under Antibiotics.","authors":"M Álvarez-Zapata, M J García-Soto, A Salazar-Martínez, R Soria-Guerra, D Portales-Pérez, C Leija-Cuevas, C Godínez-Hernández, B Juárez-Flores, H Bach, F Martinez-Gutierrez","doi":"10.1007/s12602-025-10735-8","DOIUrl":"https://doi.org/10.1007/s12602-025-10735-8","url":null,"abstract":"<p><p>Upper respiratory tract infections (URTI) in children are a problem with a remarkably diverse etiology. In most cases, the treatment requires oral antibiotics that will alter the intestinal microbiota, causing dysbiosis and diarrhea associated with such prescription. Ingesting probiotics during the therapy is recommendable to aid in modulating the microbiota. Prebiotics enhance these beneficial microorganisms. We evaluated the synbiotic effect between the probiotic yeast Saccharomyces boulardii CNCM I-745 and fructans from Agave salmiana of proven prebiotic activity on 3- to 10-year-old children diagnosed with URTI and treated with amoxicillin/clavulanic acid. First, we extracted fructans from A. salmiana, completed food safety and inflammatory response analyses, and evaluated their effect in vitro on S. boulardii under anaerobic conditions. Then, we formulated a synbiotic that was (a) included in the diet of children with URTI and taking the antibiotic, (b) excluded in a control group with URTI and under antibiotic, and (c) included in a reference group of healthy children. The extracted fructans from A. salmiana were safe for human consumption and stimulated the growth in vitro of S. boulardii, with parameters, biomass yields, and short-chain fatty acids (SCFA) comparable with inulin. The children consuming the synbiotic ingested it once daily for 1 week and tolerated it well. They had higher concentrations of beneficial microorganisms and secretory immunoglobulin A (sIgA) in stool samples with more acidic pH. Compared to those without the synbiotic, these positive effects were significant in children taking the antibiotic and the synbiotic, contributing to their favorable evolution without diarrhea.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus plantarum 1-2-3 Ameliorates the Corticosterone-Induced Fatty Liver Hemorrhagic Syndrome in Laying Hens by Biosynthesizing Gamma-Aminobutyric Acid.","authors":"Shuo Zhang, Peichao Gao, Qunxin Wang, Shuhui Liu, Sheila Okoth, Meihua Zhang, Helong Ji, Liping Yan, Wentao Fan, Suquan Song","doi":"10.1007/s12602-025-10759-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10759-0","url":null,"abstract":"<p><p>Fatty liver hemorrhagic syndrome (FLHS) has become a common and serious disease in intensive laying hen farming, seriously endangering the productivity and health of laying hens. Although probiotics have been recognized as an emerging candidate therapeutic strategy for liver disease, their effective substances and mechanisms for alleviating FLHS remain to be elucidated. In this study, corticosterone (Cort) was applied to induce FLHS in laying hens, and Lactobacillus plantarum (L. plantarum 1-2-3) was used for the intervention. The results showed that L. plantarum 1-2-3 treatment significantly ameliorated Cort-induced hepatic steatosis and liver damage in laying hens, as evidenced by decreased levels of triglyceride (TG), total cholesterol (TC), aspartate aminotransferase, and alanine aminotransferase. Importantly, we found that L. plantarum 1-2-3 could biosynthesize gamma-aminobutyric acid (GABA) by expressing glutamic acid decarboxylase. Surprisingly, feeding GABA to laying hens replicated the effect of L. plantarum 1-2-3 in alleviating Cort-induced FLHS. GABA significantly down-regulated the mRNA expression of Acc, Fasn, Cd36, and Gpam while upregulating the levels of Ppara and Cpt1a. An in vitro verification study showed that GABA decreased TG and TC content in Leghorn male hepatoma cells. Moreover, GABA significantly enhanced antioxidant enzyme activity, inhibited oxidative stress in hepatocytes, regulated the AMPK/NLRP3 signaling pathway, and reduced the protein expression of NF-κB, TNFα, and IL-1β. In conclusion, our study demonstrated that L. plantarum 1-2-3 could effectively alleviate FLHS in laying hens, while GABA was an important postbiotic for L. plantarum 1-2-3 to exert lipid-lowering and anti-inflammatory effects.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Properties of Lactic Acid Bacteria Postbiotics Isolated from Tarhana Samples.","authors":"Ecem Aydin, Çisem Bulut Albayrak, Olcay Boyacioğlu","doi":"10.1007/s12602-025-10748-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10748-3","url":null,"abstract":"<p><p>This study aimed to characterize postbiotics derived from lactic acid bacteria (LAB) isolated from tarhana, a traditional fermented Turkish soup consumed after cooking. LAB isolates from tarhana were used to prepare postbiotics through two methods: In the first of these, cell-free supernatants (CFS) were obtained by centrifugation method. In the second method, due to the fact that tarhana is consumed by cooking, heat-killed (HK) cell fragments of LAB isolates were obtained. The antimicrobial activity of the postbiotics against Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella Typhimurium was evaluated by well diffusion. A total of 150 postbiotic samples were obtained from 75 LAB isolates, with 41 inhibiting S. aureus and 18 inhibiting L. monocytogenes growth. The antimicrobial activity diminished after pH neutralization, indicating acidic compounds as the active agents. Ten postbiotic samples with the highest antimicrobial activity were selected and were identified as Pediococcus pentosaceus via 16S rRNA sequencing. DPPH Radical scavenging activity ranged from 8 and 75 µg/ml. Cytotoxicity assays showed that 24-h treatment of HT-29 cells with 20 mg/ml of P1-CFS, P2-CFS, P1-HK, and P2-HK reduced cell viability by 66%, 78%, 75%, and 72%, respectively, with IC₅₀ values ranging from 8.52 to 9.20 mg/ml. HPLC and FT-IR analyses reveled no significant differences in activity or structure between the CFS and HK forms of postbiotics (p > 0.05). In conclusion, postbiotics from tarhana demonstrated significant potential for a broad range of applications in the food and health industries, offering advantages over probiotics.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Molecular Insights into the Response of Multidrug-Resistant Helicobacter pylori to Human Neutrophil Peptide 1 (HNP-1).","authors":"Zarith Nameyrra Md Nesran, Alfizah Hanafiah, Asif Sukri, Nur Azlina Mohd Fahami, Raja Affendi Raja Ali, Bruno Silvester Lopes","doi":"10.1007/s12602-025-10747-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10747-4","url":null,"abstract":"<p><p>Multidrug-resistant (MDR) H. pylori infections present significant challenges in treatment, driving the need for novel therapeutic agents. Human neutrophil peptide 1 (HNP-1), an antimicrobial peptide, has shown potential activity against antibiotic-resistant pathogens, yet its specific efficacy and mechanisms against MDR H. pylori remain unexplored. The objective of this study was to evaluate the antibacterial activity of HNP-1 against multidrug-resistant (MDR) H. pylori. This study investigated the effects of HNP-1 on MDR H. pylori through a combination of in vitro and in silico approaches, including minimum inhibitory concentration (MIC) assays, molecular docking, and RNA sequencing. The MIC assay revealed that H. pylori strains exhibited high resistance to HNP-1 at 512 μg/mL, highlighting the need to understand this interaction at a molecular level. Molecular docking analysis identified key protein targets, RdxA, 23S rRNA, GyrA, and GyrB with varying binding affinities to HNP-1, suggesting potential pathways impacted by the peptide. RNA sequencing further revealed significant transcriptomic changes, with the ribosomal pathway and other metabolic pathways significantly upregulated upon treatment with HNP-1. These findings provide insights into H. pylori's adaptive responses to HNP-1, enhance our understanding of its interactions with MDR H. pylori strains, and highlight pathways that may serve as future therapeutic targets, underscoring the need for continued research into AMPs as complementary therapeutic strategies.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo and In Vitro Evaluation of Probiotic and Postbiotic Strategies Against Foulbrood in Honeybees.","authors":"Clémentine Mathien, Laura Fourel, Juliette Gilbert, Christophe Portelli, Khaled Fadhlaoui, Marie Diogon, Frédéric Delbac, Catherine Texier, Eric Peyretaillade, Pascale Goupil, Jean Yves Berthon, Assia Dreux-Zigha, Hicham El Alaoui","doi":"10.1007/s12602-025-10739-4","DOIUrl":"https://doi.org/10.1007/s12602-025-10739-4","url":null,"abstract":"<p><p>American and European foulbroods, which are among the most severe honeybee diseases, are caused by Paenibacillus larvae and Melissococcus plutonius, respectively. Both bacteria pose a significant threat to larval health and colony survival. Due to the lack of safe and effective treatments, there is growing interest in using beneficial bacterial supplements as a promising alternative to antibiotics. This study evaluated both the probiotic and postbiotic potential of selected bacterial strains against foulbrood pathogens with the aim of gaining a better understanding of their modes of action. An initial screening of 25 strains for anti-foulbrood activity using agar diffusion techniques was conducted to select the most active candidates for further investigation. We investigated the role of their cell-free supernatants (CFS) and initiated the characterisation of their activity and mechanisms of action. The minimal inhibitory dose of the CFS, the nature of the active compounds, and their antibacterial effect were determined. Finally, the probiotic and postbiotic properties were assessed using P. larvae-infected larvae reared under laboratory conditions. Five lactic acid bacteria exhibited antagonistic activity against one or both pathogens, with two of them showing particularly strong inhibitory effects through their CFS. The CFS of Lactobacillus crispatus and Lactiplantibacillus plantarum achieved complete inhibition of P. larvae at a concentration of 12.5% (v/v). Subsequent characterisation suggested that their activity was primarily bacteriostatic, likely mediated by lactic acid. In vivo assays demonstrated a significant 42.5% increase in larval survival when supplemented with live L. plantarum, whereas CFS treatments failed to rescue infected larvae. These results emphasise the potential of probiotic- and postbiotic-based strategies as a sustainable approach to managing foulbrood in beekeeping.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Synthetic Probiotic Bacteria for In Situ Delivery of Anti-SARS-CoV-2 Nanobodies.","authors":"Carolina E Portero, Claire Smith, Yuxi Zhou, M Raquel Marchán-Rivadeneira, Shiyong Wu, Yong Han","doi":"10.1007/s12602-025-10758-1","DOIUrl":"https://doi.org/10.1007/s12602-025-10758-1","url":null,"abstract":"<p><p>SARS-CoV-2 viral infection can be inhibited by blocking the interaction between the viral spike protein and the human receptor angiotensin-converting enzyme 2 (hACE2). The development of specific spike inhibitors using nanobodies, the antigen-binding region of llamas' antibodies, arose as a promising therapeutic method against SARS-CoV-2. However, one limitation of nanobodies is that they cannot be used directly in the human body due to their susceptibility to degradation. Bacteria-based delivery systems provide site-specific targeted action that can circumvent nanobody degradation. Here, we report the development of a genetically modified bacterium expressing anti-SARS-CoV-2 nanobodies that can inhibit the interaction between the hACE2 receptor and the receptor-binding domain (RBD) of the spike protein. Lactococcus lactis, a human symbiont probiotic bacterium, was selected to express nanobodies attached to their cell surface. Our data shows that FLAG-tagged anti-SARS-CoV-2 nanobodies were detected on the cell surface of recombinant L. lactis strains by flow cytometry and immunofluorescence without permeabilization. Furthermore, nanobodies are functional and can bind the RBD region from the spike protein in a dose-dependent manner. Inhibition of the hACE2-RBD interaction in cellular assays was quantified using a pseudotype lentivirus that mimics SARS-CoV-2 in an adaptation of the neutralization assay. Our results suggested that the recombinant bacteria can inhibit viral infectivity in more than 50% compared with a control without bacteria in a neutralization assay. These outcomes suggest that the engineered strain can be used in the future as a new therapeutic tool in COVID-19 prevention.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genesis, Health Benefits, and Future Perspectives of Probiotics: Exploring Endogenous and Exogenous Classes, Innovations, and Research Gaps.","authors":"Alice Njolke Mafe, Obiekezie Obumneme Smart, Great Iruoghene Edo, Patrick Othuke Akpoghelie, Tayser Sumer Gaaz, Emad Yousif, Endurance Fegor Isoje, Ufuoma Augustina Igbuku, Shams A Ismael, Arthur Efeoghene Athan Essaghah, Dina S Ahmed, Dilber Uzun Ozsahin, Huzaifa Umar","doi":"10.1007/s12602-025-10756-3","DOIUrl":"https://doi.org/10.1007/s12602-025-10756-3","url":null,"abstract":"<p><p>Probiotics are live beneficial microorganisms that confer health benefits to the host when administered in adequate amounts, have gained considerable scientific and commercial interest for their ability to support gut health, strengthen immunity, and reduce disease risk. This review traces the genesis of probiotic science from its origins in traditional fermented foods to contemporary clinical applications, offering a conceptual understanding of its evolution. A clear distinction is drawn between endogenous probiotics, naturally resident in the human microbiome, and exogenous probiotics, introduced via dietary supplements and functional foods. The broad spectrum of documented health benefits is examined, encompassing digestive, immune, metabolic, neurological, and dermatological outcomes. Recent innovations are highlighted, including bioengineered probiotic strains with targeted therapeutic functions, the integration of probiotics with prebiotics as synbiotics, and advanced delivery systems such as microencapsulation and nanotechnology. Special attention is given to regulatory frameworks, with global comparisons and a focused case study on Argentina, alongside a structured roadmap for translating research into market-ready products. The review also addresses inclusivity in probiotic use, emphasizing safety considerations across diverse populations, and underscores the strain-specific nature of probiotic effects. Current challenges such as commercialization gaps, regulatory inconsistencies, and underexplored applications in non-digestive health domains are critically discussed. The conclusion calls for interdisciplinary collaboration among microbiologists, nutritionists, clinicians, and technologists to accelerate innovation, ensure equitable access, and maximize the potential of probiotics in promoting health and preventing disease.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects and Mechanisms of Lactiplantibacillus plantarum G83 on Enterotoxigenic Escherichia coli (ETEC)-Induced Intestinal Inflammation.","authors":"Chunjia Li, Miao Wang, Lixiao Duan, Jinge Xin, Xueqin Ni, Dong Zeng, Bangyuan Wu","doi":"10.1007/s12602-025-10712-1","DOIUrl":"https://doi.org/10.1007/s12602-025-10712-1","url":null,"abstract":"<p><p>Enterotoxigenic Escherichia coli (ETEC) is a prevalent intestinal pathogen that significantly impacts both human and animal health. G83, isolated from giant panda feces, has demonstrated notable probiotic properties. In this study, C57BL/6 J mice were randomly divided into Control, ETEC, and G83 groups. Experimental included monitoring body weight, assessing fecal occult blood, histopathological examination of ileal tissues, and quantification of antioxidant markers (SOD, T-AOC, MDA) in ileal tissues. Furthermore, real-time quantitative PCR was utilized to determine mRNA expression levels of inflammatory cytokines (TNF-α, IL-17, IL-10), tight junction proteins (Claudin, ZO-1, Occludin), mucin (Muc2), and lysozyme (Lyz-1). Transcriptomic bioinformatics analysis and 16S rRNA sequencing were integrated to characterize host gene expression profiles and gut microbial compositional dynamics, respectively. The results revealed that G83 alleviated ETEC-induced weight loss, reduced fecal occult blood, and mitigated ileal structural injuries. Additionally, G83 significantly enhanced intestinal antioxidant capacity by increasing T-AOC and SOD levels. Mechanistically, G83 downregulated pro-inflammatory cytokines TNF-α and IL-17 and the levels of Muc2 and Lyz1, while upregulating the expression of tight junction proteins ZO-1, Claudin, and Occludin. Transcriptomic analysis suggests that ETEC triggers inflammasome activation and initiates inflammatory responses by significantly upregulating Aim2. Conversely, G83 exerts protective effects by modulating the immune regulatory network-specifically, by significantly downregulating C3 expression to activate the complement system and participating in mucosal immune remodeling. Enrichment analysis reveals that G83 alleviates ETEC-induced intestinal inflammation primarily by inhibiting the NF-κB pathway and enhancing the intestinal IgA immune network. Additionally, 16S rRNA analysis indicates that G83 may improve ETEC-induced alterations in microbial community structure by increasing the abundance of beneficial bacteria (e.g., Lactobacillus), thereby further ameliorating impairment of intestinal microbial barrier function in mice. These findings provide a scientific basis for using G83 to ameliorate ETEC-mediated intestinal inflammation.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trp-containing Peptides with Therapeutic Potential for Cutibacterium acnes Infection.","authors":"Mengmiao Li, Zhuyun Liu, Jialu He, Jiahuan Jiang, Dejing Shang, Weibing Dong","doi":"10.1007/s12602-025-10749-2","DOIUrl":"https://doi.org/10.1007/s12602-025-10749-2","url":null,"abstract":"<p><p>Cutibacterium acnes (C. acnes, formerly classified as Propionibacterium acnes) is a Gram-positive bacterium that contributes to the development of acne vulgaris, resulting in inflammation and pustule formation on the skin. In this study, we developed and synthesized a series of antimicrobial peptides (AMPs) that are derived from the skin secretion of Rana chensinensis. Screening by MIC assay, Trp-containing peptides had potent anti-bacterial activity against C. acnes. The effect of Trp-containing peptides on the morphology was detected by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The cell surface of C. acnes that was treated with Trp-containing peptides appeared rough and irregular. Further investigations into the peptides effects on cell membranes were conducted via zeta potential, Disc3(5) and Live/Dead assays. The results revealed that the AMPs bound to the cell surface, induced the disruption of the cell membranes and ultimately led to cell death. The leakage of cellular contents like nucleic acid, K⁺ and Ca²⁺ also confirmed the membrane-damaging effect of Trp-containing peptides. In addition, Trp-containing peptides suppressed biofilm formation and disrupted mature biofilms of C. acnes. Among the Trp-containing peptides, I4WL5W with lowest cytotoxic effect displayed dual anti-bacterial and anti-inflammatory effects in acne mice; it decreased the bacterial load and inhibited the levels of TNF-α, IL-6 and IL-1β in the infected skin. This study demonstrated that Trp-containing peptides might be promising candidates for treating acne.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}