Three Characterized Probiotics from Dairy Sources-Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), and Leuconostoc lactis (RM 2.1)-Exhibit Potential for Antidiarrheal Effects.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-24 DOI:10.1007/s12602-025-10775-0

Jannatul Ferdous, Md Saddam Hossain, Palash Kumar Sarker, Md Shimul Bhuia, Md Sakib Al Hasan, Raihan Chowdhury, Md Sarafat Ali, Md Robiul Auwal, Keshob Chandra Das, Muhammad Torequl Islam, Abu Hashem, Md Salimullah

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Abstract

Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.

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从乳制品中提取的三种特性益生菌——猪囊菌（ProY 5.1）、乳酸肠球菌（ProY 5.3）和乳酸白杆菌（RM 2.1）——显示出潜在的止泻作用。

益生菌是有益的活微生物，可以提供安全的管理几种疾病，如腹泻。目前的研究旨在通过体外和体内方法评估潜在益生菌的止泻活性。从乳制品中分离菌株并进行益生菌鉴定，采用体外培养法筛选出4株菌株，分别为：Weissella confusa （ProY 5.1）、lactoenterococcus （ProY 5.3）、Leuconostoc lactis （RM 2.1）和Bacillus subtilis （ProY 11.3）。这些菌株表现出非溶血特性，耐酸性，对胆盐和酚的耐受性，强粘附率，益生菌活性和对六种肠道病原体的抗菌活性。利用分子技术MALDI-TOF和全基因组测序对潜在菌株进行鉴定。蓖麻油致腹泻瑞士白化小鼠的体内实验显示，与洛哌丁胺和次水杨酸铋作为阳性对照相比，蓖麻油对腹泻瑞士白化小鼠有积极的影响，而PBS + MRS肉汤作为阴性对照。筛选的ProY 5.1、ProY 5.3和RM 2.1显著延长了潜伏期，减轻了腹泻症状(p

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.