{"title":"从乳制品中提取的三种特性益生菌——猪囊菌(ProY 5.1)、乳酸肠球菌(ProY 5.3)和乳酸白杆菌(RM 2.1)——显示出潜在的止泻作用。","authors":"Jannatul Ferdous, Md Saddam Hossain, Palash Kumar Sarker, Md Shimul Bhuia, Md Sakib Al Hasan, Raihan Chowdhury, Md Sarafat Ali, Md Robiul Auwal, Keshob Chandra Das, Muhammad Torequl Islam, Abu Hashem, Md Salimullah","doi":"10.1007/s12602-025-10775-0","DOIUrl":null,"url":null,"abstract":"<p><p>Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Three Characterized Probiotics from Dairy Sources-Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), and Leuconostoc lactis (RM 2.1)-Exhibit Potential for Antidiarrheal Effects.\",\"authors\":\"Jannatul Ferdous, Md Saddam Hossain, Palash Kumar Sarker, Md Shimul Bhuia, Md Sakib Al Hasan, Raihan Chowdhury, Md Sarafat Ali, Md Robiul Auwal, Keshob Chandra Das, Muhammad Torequl Islam, Abu Hashem, Md Salimullah\",\"doi\":\"10.1007/s12602-025-10775-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.</p>\",\"PeriodicalId\":20506,\"journal\":{\"name\":\"Probiotics and Antimicrobial Proteins\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Probiotics and Antimicrobial Proteins\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s12602-025-10775-0\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Probiotics and Antimicrobial Proteins","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12602-025-10775-0","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Three Characterized Probiotics from Dairy Sources-Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), and Leuconostoc lactis (RM 2.1)-Exhibit Potential for Antidiarrheal Effects.
Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.
期刊介绍:
Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.