Poultry Science最新文献

{"title":"Modulation of early life gut microbiota through inclusion of earthworms and vermicompost in broiler diets","authors":"Muhammad Zeeshan Akram ,&nbsp;Oyekunle John Oladosu ,&nbsp;Nadia Everaert ,&nbsp;Cornelia C. Metges ,&nbsp;Gürbüz Daş","doi":"10.1016/j.psj.2025.105121","DOIUrl":"10.1016/j.psj.2025.105121","url":null,"abstract":"<div><div>We previously showed that providing earthworms (<strong>EW</strong>) to broilers during early life can mitigate dietary challenges induced by soluble non-starch polysaccharides (<strong>NSP</strong>). However, whether the positive effects of providing EW are associated with changes in the gut microbial communities of broilers was not studied. This follow-up study investigated the influence of providing EW and vermicompost (<strong>VC</strong>) on gut microbiota diversity in broilers fed either a standard corn-soy based diet as positive control (<strong>CON+</strong>) or a diet rich in NSP as negative control (<strong>CON-</strong>). A total of 120 newly hatched male birds of Cobb-500 genotype were examined in two periods (<strong>P</strong>), each lasting 8 days. In P1, birds were divided into four groups: two groups received the CON+ diet (n = 30 each), the third group received CON+ plus 1 % EW (<strong>CON+EW</strong>, n = 30), and the last group received CON+ supplemented with 1 % VC (<strong>CON+VC</strong>, n = 30). Half of the birds in each group were euthanized at the end of P1, and ileal digesta were collected for microbiota analysis. In P2, one of the CON+ groups from P1 continued the same diet, while the remaining groups were switched to dietary challenge either NSP supplemented negative control CON- (n = 15), or <strong>CON-EW</strong> (n = 15) or <strong>CON-VC</strong> (n = 15). At the end of P2, the remaining birds in all groups were euthanized for ileal digesta collection. Microbial composition was assessed using 16S rRNA gene sequencing. In P1, the CON+VC exhibited a significantly higher Chao1 index compared to the CON+ and CON+EW (<em>P</em> &lt; 0.05). In P2, α-diversity metrics remained unchanged across groups (<em>P</em> &lt; 0.05), although the Chao1 index in the CON+ showed a trend toward an increase (<em>P</em> = 0.078). Analysis of β-diversity highlighted significant differences between dietary groups in P2 (<em>P</em> = 0.001). Further analysis identified differentially enriched genera, revealing that <em>Enterococcus</em> was prominent in the CON+ during P1, while <em>Lactobacillus</em> was significantly higher in the CON+EW group. In P2, the CON-EW group exhibited increased <em>Lactobacillus</em> abundance while <em>Escherichia-Shigella</em> was overrepresented in the CON- group. Functional analysis showed that the CON-EW and CON-VC diets enriched the pathways related to Nicotinamide Adenine Dinucleotide biosynthesis and fermentation to acetate and lactate, whereas the CON- increased the biosynthesis of enterobactin and aerobactin. In conclusion, dietary earthworm supplementation positively influenced gut microbiota composition and predicted functions in response to dietary challenges.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105121"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of energy density of diets and dietary Pediococcus acidilactici supplementation on productive performance, egg quality, and intestinal function in laying hens","authors":"Airong Dong ,&nbsp;Xuemei Ding ,&nbsp;Jianping Wang ,&nbsp;Qiufeng Zeng ,&nbsp;Shiping Bai ,&nbsp;Yue Xuan ,&nbsp;Shanshan Li ,&nbsp;Sharina Qi ,&nbsp;Xiaojuan Bi ,&nbsp;Chao He ,&nbsp;Keying Zhang","doi":"10.1016/j.psj.2025.105118","DOIUrl":"10.1016/j.psj.2025.105118","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of supplementing <em>Pediococcus acidilactici</em> CNCM I-4622 MA 18/5 M (<strong>PA</strong>) into diets with two energy levels on the production performance and intestinal function of Lohmann pink laying hens from 49 to 65 weeks of age. A total of 1400 hens were used in this study. A 2 × 2 + 1 experimental design with 2 metabolic energy levels (2700.00 kcal/kg, <strong>NE</strong>; 2625.00 kcal/kg, <strong>LE</strong>), two PA levels (0 mg/kg, 110 mg/kg), and one group with intermittent PA supplementation (<strong>LE-0/PA</strong>). The experiment comprised 5 treatments with 14 replications each, and each replication had 20 hens. The results showed that reducing the dietary energy level tended to decrease body weight (<em>P</em> = 0.056). However, supplementing PA in low-energy diets tended to increase body weight in 16 weeks (<em>P</em> = 0.063). The LE-0/PA group had higher laying rates and feed utilization rates compared to other groups (<em>P</em> &gt; 0.05), maybe resulting in greater economic benefits. The LE group had significantly lower eggshell strength, Haugh unit, trypsin activity, lipase activity, and tibia breaking strength compared to the NE group (<em>P</em> &lt; 0.05). Dietary supplementation with PA significantly increased eggshell strength (<em>P</em> &lt; 0.05), tibia breaking strength, villus height/crypt depth ratio (<strong>VH/CD</strong>), lipase activity, and secretory immunoglobulin A (<strong>SIgA</strong>) content in the jejunum (<em>P</em> &lt; 0.05). Additionally, PA supplementation significantly reduced the expression of interleukin 8 (<strong><em>IL-8</em></strong>), tumor necrosis factor-α (<strong><em>TNF-α</em></strong>), toll-like receptor 4 (<strong><em>TLR-4</em></strong>), and nuclear factor kappa-B (<strong><em>NF-κB</em></strong>) mRNA (<em>P</em> &lt; 0.05). In conclusion, reducing the dietary energy level of hens can reduce the body weight and feed utilization efficiency. However, dietary PA supplementation improved body weight, reduced the expression of intestinal inflammation-related factors, and enhanced intestinal health, particularly in low-energy diets. Intermittent PA supplementation may enhance performance benefits, suggesting its potential as a strategy to optimize production performance and intestinal health in hens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105118"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Butyric acid from ligilactobacillus animalis 2020MB acts on membrane BamA to control avian pathogenic escherichia coli","authors":"Haichang Yin ,&nbsp;Zunhe Du ,&nbsp;Xinjie Jiang ,&nbsp;Yao Zhou ,&nbsp;Zhenhua Jin ,&nbsp;Feng Cong","doi":"10.1016/j.psj.2025.105119","DOIUrl":"10.1016/j.psj.2025.105119","url":null,"abstract":"<div><div>Avian pathogenic <em>Escherichia coli</em> can cause high morbidity, mortality, and serious economic losses to the global poultry industry. Lactic acid bacteria inhibit the growth of many pathogens, including <em>E. coli</em>, but the underlying mechanism remains unclear. In this study, we investigated the effect of the cell-free supernatant of <em>Ligilactobacillus animalis</em> 2020MB isolated from the intestinal tract of chickens on specific pathogen-free chickens infected with <em>E. coli</em>. The cell-free supernatant-induced inhibition of <em>E. coli</em> infection was determined through clinical symptom observation, pathological analysis, and qPCR. Protease and heat treatments did not affect the antibacterial activity of cell-free supernatant, suggesting that an organic acid was the antibacterial substance. Liquid chromatography–mass spectrometry and non-targeted metabolomics identified antibacterial activity for eight <em>L. animalis</em> 2020MB cell-free supernatant metabolites, including butyric, valeric, and succinic acids. The inhibitory activity of butyric acid was quantified by determining the minimal inhibitory concentration. Scanning electron microscopy, laser confocal microscopy, and proteomic analysis revealed that butyric acid altered the morphology and impaired the cell envelope integrity of target bacteria, leading to leakage of intracellular contents. BamA was identified as the membrane protein target for butyric acid. The findings reveal the molecular mechanism of action of <em>L. animalis</em> 2020MB in the chicken intestine against <em>E. coli</em>.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105119"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Pyrococcus furiosus Argonaute coupled with RPA for highly sensitive and specific detection of Mycoplasma gallisepticum","authors":"Yanli Zhao ,&nbsp;Dehai Su ,&nbsp;Min Li ,&nbsp;Qin Deng ,&nbsp;Shiyu Xiong ,&nbsp;Youshu Feng ,&nbsp;Hongxia Jiang ,&nbsp;Huanzhong Ding","doi":"10.1016/j.psj.2025.105116","DOIUrl":"10.1016/j.psj.2025.105116","url":null,"abstract":"<div><div><em>Mycoplasma gallisepticum</em> (<strong>MG</strong>) is the pathogen that causes chronic respiratory disease (<strong>CRD</strong>) in chickens and infectious sinusitis in turkeys. Clinical symptoms such as cough and dyspnea cause a decrease in the egg production rate and carcass quality of chickens, which results in considerable economic losses to the poultry industry. Thus, it is urgent to establish a fast, accurate and sensitive method for detecting MG. In this study, the detection of MG by combining recombinase polymerase amplification (<strong>RPA</strong>) with argonaute from <em>Pyrococcus furiosus</em> (<strong><em>Pf</em>Ago</strong>) was established that allows visual observation under blue and UV light. The sensitivity of RPA-<em>Pf</em>Ago reached 1 copy/<em>µ</em>L. After the chicken infection model, it was found that tracheal inhalation was superior to eye and nose drips and sprays, the results of RPA-<em>Pf</em>Ago and qPCR were completely consistent. This study provides a reference tool for the monitoring of MG in the clinic and contributes to the early diagnosis of the disease.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105116"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell","authors":"Ying-ying Yang,&nbsp;Dong Dai,&nbsp;Hai-jun Zhang,&nbsp;Shu-geng Wu,&nbsp;Guang-hai Qi,&nbsp;Jing Wang","doi":"10.1016/j.psj.2025.105111","DOIUrl":"10.1016/j.psj.2025.105111","url":null,"abstract":"<div><div>This study investigated the differences of calcium transport-related proteins and metabolites in the uterus of hens with different breaking strength eggshell during the eggshell calcification. A total of 200 Hy-Line Brown laying hens, aged 75 weeks, were selected and categorized into two groups based on the eggshell breaking strength: a high-strength group (HS, &gt; 42 N) and a low-strength group (LS, &lt; 32 N). Laying hens were sampled at 1 h, 7 h (the initiation stage of eggshell calcification), and 17 h (the growth stage of eggshell calcification) post-oviposition (PO). The LS group showed a decreased thickness, weight and weight ratio of eggshells, accompanied with ultrastructural deterioration and total Ca reduction. The expression levels of <em>ATP2A3, ATP2B2, SLC8A1</em>, and <em>SLC8A3</em> were significantly increased in the HS at 17 h PO when compared to 1 h and 7 h PO, while no significant changes were observed in the LS. At 7 h PO, the LS group had lower uterine mucosa calcium levels, higher TRPV6 protein expression, and lower CALB1 protein expression. In the HS group, uterine metabolites showed a significant increase in glutathione, citrulline, and proline at 7 h PO, whereas, at 17 h PO, the tricarboxylic acid cycle pathway was significantly enriched. These findings suggest that uterine calcium transport activity is relatively subdued during the initiation stage of eggshell calcification, focusing on redox repair activities to maintain homeostasis for mammillary knobs formation. Subsequently, uterine calcium transport activity becomes highly active during the growth stage of eggshell calcification, primarily supporting rapid calcium transport through enhanced energy metabolism. In aged laying hens, the lower eggshell breaking strength may be attributed to decreased calcium levels during the initiation stage and imbalanced redox during the growth stage, which could affect calcium transport and lead to a weak ultrastructure during the calcification period.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105111"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The landscape of RNA 5-methylcytosine modification during chicken embryonic myogenesis","authors":"Hongxia Jiang ,&nbsp;Jing Liu ,&nbsp;Yuxiang Wang ,&nbsp;Zurong Liao ,&nbsp;Xiaoyun Xiao ,&nbsp;Zhenxvan Ding ,&nbsp;Xvwen Huang ,&nbsp;Wenwei Li ,&nbsp;Zikun Wang ,&nbsp;Jingzhan Liang ,&nbsp;Jiguo Xu ,&nbsp;Xiaolong Hu ,&nbsp;Huirong Mao ,&nbsp;Sanfeng Liu ,&nbsp;Biao Chen","doi":"10.1016/j.psj.2025.105109","DOIUrl":"10.1016/j.psj.2025.105109","url":null,"abstract":"<div><div>Skeletal muscle is a vital protein source for human diets, making its development a significant focus in poultry research. This study examines the effects of RNA 5-methylcytosine (m5C) modifications on chicken skeletal muscle development at two critical embryonic stages: before myoblast differentiation (E10) and after differentiation (E19). By employing MeRIP-seq, we analyzed the dynamic distribution of m5C modifications within the leg muscle transcriptome, uncovering notable differences in modification states between these two stages. Our results indicate that m5C modifications are widespread in chicken skeletal muscle transcriptome and present a unique distribution pattern. Unlike N6-methyladenosine modifications, which typically show a negative correlation with RNA expression, m5C modifications exhibited a weak positive correlation in our findings. Additionally, we identified multiple m5C peaks on important epigenetic regulators, including <em>DNMT3A, DNMT3B, DNMT1, TET3</em>, and <em>METTL3</em>. This observation suggests that RNA m5C may interact with these genes to jointly influence chicken skeletal muscle development. Furthermore, we identified key genes linked to m5C peaks that are enriched in pathways associated with cell cycle regulation, muscle growth, and lipid metabolism. This study provides valuable insights into the role of m5C modifications in the development of chicken skeletal muscle and highlights their potential for coordinating myogenesis and lipid metabolic processes.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105109"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From gut microbiota to host genes: A dual-regulatory pathway driving body weight variation in dagu chicken (Gallus gallus domesticus)","authors":"Xiaohan Li ,&nbsp;Xueru Han ,&nbsp;Huan Yan ,&nbsp;Hongyan Zhu ,&nbsp;Hongcai Wang ,&nbsp;Desheng Li ,&nbsp;Yumin Tian ,&nbsp;Yuhong Su","doi":"10.1016/j.psj.2025.105067","DOIUrl":"10.1016/j.psj.2025.105067","url":null,"abstract":"<div><div>During the growth and development of animals, there is an interaction between the gut microbiota and the host genotype. The host genotype can regulate the microbiota, and in turn, the microbiota can influence host gene expression, thereby affecting the animal's production performance. This study explored the dynamic interplay between the gut microbiota and host gene expression in body weight variation in Dagu chicken, an indigenous poultry genetic resource in China. We characterized mucosa-associated microbiota across four gastrointestinal segments (duodenum, jejunum, ileum, cecum) and ileocecal chyme microbiota in 12-week-old Dagu chickens stratified by divergent body weight phenotypes, while simultaneously quantifying region-specific intestinal epithelial transcriptional regulation. 16S rDNA sequencing was employed to identify <em>Firmicutes</em> as the predominant bacterial phylum, with notable differences in the abundance of specific genera (e.g., <em>Ligilactobacillus</em> and <em>Lactobacillus</em>) being observed between the high- or low-body-weight groups. Enhanced biosynthesis pathways were functionally predicted in heavier roosters, whereas reduced nutrient metabolism pathways were contrasted. A conserved functional concordance was observed between regionally predominant differential microbiota and the physiological specialization of corresponding intestinal niches. Functional analysis revealed that the high-body-weight group demonstrated superior capabilities in microbial biosynthesis, whereas the low-body-weight group exhibited enhanced microbial metabolic activity. <em>NAA80</em> was identified as the common differentially expressed gene across all intestinal epithelial tissues. The Gene Ontology and KEGG pathway analyses revealed elevated nutrient absorption efficiency in the high-body-weight group, while the low-body-weight group demonstrated accelerated cellular renewal rates and shorter cycles. Correlation analysis identified significant associations between gut microbiota and host genes expression profiles, with the majority of correlations being positive. These results suggest a coordinated interaction between microbial communities and host genetic regulation, potentially driving phenotypic differences in body weight performance.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105067"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drying methods affected the fatty acids oxidation and bacterial community of traditional dry-cured ducks during processing and its relation with the aroma formation","authors":"Linyue Qi ,&nbsp;Liangwei Xu ,&nbsp;Hongbing Chen ,&nbsp;Haixing Li ,&nbsp;Yikui Lai ,&nbsp;Xiaohua Liu","doi":"10.1016/j.psj.2025.105110","DOIUrl":"10.1016/j.psj.2025.105110","url":null,"abstract":"<div><div>The formation of volatile compounds in dry-cured ducks produced by hot and cold air circular drying (HCAD), hot air drying (HAD), and traditional sun drying (TSD) were analyzed by E-nose and GC-MS. The HCAD exhibited higher alcohols, aldehydes, esters, and furans than that of the other two. Six, five, and three key compounds were identified by OAV in HCAD, HAD, and TSD, respectively. Nonanal was the highest relative content of aldehydes in all groups. (<em>E</em>)-2-octenal was detected only in HCAD. HAD accelerated the oxidation of fatty acids. The POV and AV of HAD were 0.24 g/100 g and 2.02 mg/g when stored for 15 days, which were higher than that of HCAD and TSD. <em>Proteobacteria, Actinobacteriota</em> and <em>Firmicutes</em> were the most abundant phylum, <em>Psychrobacter, Kocuria</em>, and <em>Macrococcus</em> were the primary dominant genus. The hightest abundance of <em>Firmicutes</em> and <em>Macrococcus</em> in the HCAD-15d might contribute to the aroma formation. HCAD showed a better quality and flavor than TSD and HAD, indicating it is an innovative method for producing dry-cured duck.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105110"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spleen proteome profile reveals immune responses induced by dietary spray-dried plasma against Salmonella Enteritidis infection in broiler chicks","authors":"Tunde E. Ogundare ,&nbsp;Theresa M. Casey ,&nbsp;Uma K. Aryal ,&nbsp;Yewande O. Fasina","doi":"10.1016/j.psj.2025.105091","DOIUrl":"10.1016/j.psj.2025.105091","url":null,"abstract":"<div><div><em>Salmonella</em> infection severely impacts poultry health, productivity, and food safety. Spray dried plasma (SDP) shows good efficacy as an alternative to in-feed antibiotics for broiler chickens. Our objective was to identify potential mechanisms of SDP benefits by analyzing effects on spleen proteome and in response to <em>Salmonella</em> Enteritidis challenge (SE). A 2 × 2 factor study of broilers (n = 240) were assigned to CON (control) or SDP (30g/kg) diet at 1 day of age, and gavaged with tryptic soy or 7.46 × 10⁸ CFU SE /mL, resulting in 4 groups CON (n=60), SDP (n=60), CON-SE (n=60) and SDP-SE (n=60). On d 2 and d 5 post-challenge, chicks (n=4/treatment) were euthanized, and spleen collected to isolate proteins, and measure using label-free liquid chromatography tandem mass spectrometry. Data were mapped to <em>Gallus gallus</em> Uniprot database, and differentially abundant proteins (DAP; <em>P</em>&lt;0.05) identified using ANOVA. Across all samples, 4,300 proteins were identified, and SDP treatment resulted 116 DAP. Proteins (56) that decreased were associated with positive regulation of I-kappaß kinase/NF- kappaß signaling and positive regulation of apoptosis process. Proteins that increased (60) were associated with integral components of endoplasmic reticulum membrane and necroptosis pathway. Analysis of the interaction between SDP and SE challenge found 119 DAP. The proteins that increased (87) were associated with RNA binding, ATP binding, mRNA splicing, and protein phosphorylation. Proteomic signatures of spleen reveals that the actions of SDP in broiler chicks are anti-inflammatory and anti-apoptotic and predicted to inhibit pro-inflammatory response through the inhibition of NF-ĸB. However, in the presence of <em>Salmonella</em> infection, it promotes regulated inflammatory response.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105091"},"PeriodicalIF":3.8,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary non-phytate phosphorus and phytase interaction affects growth performance, mineral transportation and metabolism in broiler chickens","authors":"Bingbing Ma ,&nbsp;Yan Zhang ,&nbsp;Mengyuan Liu ,&nbsp;Chuanxin Shi ,&nbsp;Yu Zhou ,&nbsp;Mengyue Chen ,&nbsp;Yuchun Chang ,&nbsp;Hongxia Qu ,&nbsp;Liao Zhang ,&nbsp;Chunmei Zhang ,&nbsp;Guanhua Chen ,&nbsp;Jincheng Han","doi":"10.1016/j.psj.2025.105108","DOIUrl":"10.1016/j.psj.2025.105108","url":null,"abstract":"<div><div>The interaction effects of dietary non-phytate phosphorus (<strong>NPP</strong>) and phytase on growth performance, mineral metabolism, and gene expressions of mineral transporters in broilers duodenum and kidney (0-3 weeks) were studied. The levels of NPP (0.45%, 0.35%, or 0.25%) and phytase (0 or 2,500 FTU/kg) in the diets were applied in a 3 × 2 factorial arrangement to carry out rearing feeding strategies. Broilers received the diet containing 0.25% NPP showed lower (<em>P</em> &lt; 0.05) body weight gain (<strong>BWG</strong>), feed intake (<strong>FI</strong>), plasma calcium (<strong>Ca</strong>) and phosphorus (<strong>P</strong>) levels, bone attributes, and higher (<em>P</em> &lt; 0.05) mortality rate than those of broilers fed diets containing 0.45% NPP alone or in combination of phytase, 0.35% NPP or 0.25% NPP in combination of phytase. Significant interactions (<em>P</em> &lt; 0.05) of phytase and NPP were found in BWG, FI, bone attributes, and plasma Ca and P levels and mortality rate of broiler. Broilers received the diet containing 0.25% NPP had higher (<em>P</em> &lt; 0.05) mRNA expressions of nuclear vitamin D receptors (<strong>nVDR</strong>), Calbindin-D28k (<strong>CaBP-D28k</strong>) and Plasma membrane Ca ATPase 1b (<strong>PMCA1b</strong>) in duodenum, mRNA expressions of CaBP-D28k and sodium-dependent phosphate cotransporters type IIa (<strong>NaPi-IIa</strong>) in kidney, and liver 25-hydroxylase mRNA expression than those of broilers fed diets containing 0.45% NPP alone or in combination of phytase, 0.35% NPP or 0.25% NPP in combination of phytase. The interaction of NPP and phytase significantly affected (<em>P</em> &lt; 0.05) the mRNA expressions of nVDR, CaBP-D28k and PMCA1b in duodenum, the mRNA expressions of CaBP-D28k and NaPi-IIa in kidney, and liver 25-hydroxylase mRNA expression. Dietary phytase supplementation improved growth performance, bone attributes, and absorption of Ca and P even if dietary NPP level dropped to 0.25%.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105108"},"PeriodicalIF":3.8,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}