Poultry Science最新文献

{"title":"Transcriptional activity and variation analysis of genes critical for long-chain fatty acid (C≥16) elongation and desaturation in Pekin ducks","authors":"Dandan Sun ,&nbsp;Yongtong Liu ,&nbsp;Xiaoqin Li,&nbsp;Mengqi Ge,&nbsp;Meiyi Zhu,&nbsp;Yuqin He,&nbsp;Zhuocheng Hou","doi":"10.1016/j.psj.2024.104437","DOIUrl":"10.1016/j.psj.2024.104437","url":null,"abstract":"<div><div>To deepen our understanding of long-chain fatty acid carbon chain elongation and desaturation in ducks, this study systematically analyzed the transcriptional activities of key gene promoters, including <em>ELOVL</em>s, <em>FADS</em>s, and <em>SCD</em>s. Predictive modeling coupled with statistical analysis revealed a prevalence of binding motifs for transcription factors, particularly those associated with <em>Sp1, NF-1</em>, and <em>C/EBPalpha</em>. Moreover, variation analysis of resequencing data from both wild and domestic ducks, specifically mallards and Pekin ducks, informed targeted mutagenesis within the core promoter regions of <em>ELOVL2, ELOVL5</em>, and <em>ELOVL6</em>. Notably, mutations at positions -56 G&gt;C in <em>ELOVL2</em>, -52 T&gt;C in <em>ELOVL5</em>, and -513 T&gt;C in <em>ELOVL6</em> significantly diminished transcriptional activity. These findings substantially enhance our understanding of the molecular mechanisms regulating the biosynthesis of long-chain fatty acids in ducks and support future genetic selection initiatives aimed at developing Pekin duck breeds with enhanced nutritional value.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104437"},"PeriodicalIF":3.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplement of veratric acid alleviates liver steatosis and reduces abdominal fat deposition in broilers.","authors":"Tingli Pan, Siqi Liu, Qichao Liao, Yu Li, Yang Xiao, Yu Sun, Lei Zhou, Yixing Li","doi":"10.1016/j.psj.2024.104406","DOIUrl":"10.1016/j.psj.2024.104406","url":null,"abstract":"<p><p>Nonalcoholic fatty liver disease (NAFLD) and obesity are nutritional metabolic diseases that are prevalent in the poultry industry, and have a negative impact on its functioning. Veratric acid (VA) is a phenolic acid compound extracted from the Chinese herbal medicine Trollius chinensis Bunge, known for its anti-inflammatory and antioxidant properties. In this study, we used chicken hepatocytes (Leghorn male hepatoma cells) and treated with a mixture of oleic acid and palmitic acid as well as Yellow-feathered broilers fed a high-fat diet to examine the impact of VA on liver-lipid metabolism and deposition of abdominal fat. The results showed that VA (1μM) reduced the triglyceride and total cholesterol levels in the chicken hepatocytes (p < 0.05). In the broiler NAFLD model, VA significantly reduced liver TG levels (p < 0.05) without affecting growth performance. Dietary supplementation with 0.05% or 0.1% VA supplementation also significantly reduced the mRNA expression levels of key genes involved in the synthesis of fatty acids such as sterol regulatory element-binding protein 1c, fatty acid synthase, and acetyl-CoA carboxylase in broiler livers. In addition, 0.1% VA reduced abdominal fat accumulation and improved blood biochemical indexes in broilers. Network pharmacology analysis suggested that VA may participate in regulating fat metabolism in broilers via the proliferator-activated receptor signaling pathway. Taken together, the study results support VA as a candidate feed additive to provide a novel strategy for preventing NAFLD and excessive fat deposition in chickens.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104406"},"PeriodicalIF":3.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic impact of inclusion of black soldier fly products in broiler diets: A comparison between conventional and higher animal welfare production systems in the Netherlands","authors":"Mark Leipertz,&nbsp;Henk Hogeveen,&nbsp;Helmut Saatkamp","doi":"10.1016/j.psj.2024.104411","DOIUrl":"10.1016/j.psj.2024.104411","url":null,"abstract":"<div><div>The primary objectives of this study were to analyze economic feasibility of incorporating black soldier fly products in broiler feed and to compare the viability of alive (alive grown larvae (<strong>AGL</strong>)) or processed (defatted larvae meal (<strong>DLM</strong>) and insect oil) insect inclusion into the two Dutch broiler welfare systems: conventional and better life one star (<strong>BLS</strong>).</div><div>An existing simulation model for insect production was adapted to build an economic model for the insect-fed broiler breast meat supply chain. Amounts, full costs, and breakeven prices of different production setups were compared and the impacts of specific input parameters were tested by sensitivity and breakeven analyses.</div><div>Results indicated a hypothetical demand of 2.15 million tons wet matter of raw substrate to meet the total Dutch breast meat production (BLS broilers and 10% AGL inclusion). In case of 10% processed insect inclusion, the conventional broiler breast meat price increased from 3,947 to 6,069 €/t (53.7%) and the BLS breast meat price from 6,493 to 9,662 €/t (48.8%). Alive insect inclusion resulted in a smaller cost increase (42.2%) by skipping the costly processing step. To become price competitive, DLM or AGL must cost 618 or 801 €/ton dry matter (<strong>tDM</strong>), respectively, requiring a raw substrate mix price of −109 €/tDM.</div><div>High input amounts and costs currently hinder the widespread adoption of insect products in Dutch mass poultry feed. However, in the long run, using negative value substrates or targeting niche markets for broiler welfare with low AGL inclusion levels could become viable business models.</div><div>Research is needed on the impact of negative value substrates on insect production performance and contamination risks. Furthermore, the impact of varying AGL composition requires special attention.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104411"},"PeriodicalIF":3.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of barley processing and enzyme supplementation on broiler performance, gut morphometry, and the occurrence of ascites syndrome in broiler chickens.","authors":"Mina Zohrabi, Fariborz Khajali, Behrouz Dastar","doi":"10.1016/j.psj.2024.104410","DOIUrl":"10.1016/j.psj.2024.104410","url":null,"abstract":"<p><p>An experiment was conducted to evaluate the nutritive value of processed barley grains and their influence upon growth performance, gut function, and the occurrence of ascites syndrome in broiler chickens. Day-old broilers (n=504) were randomly assigned to six treatments (3 levels of grain processing × 2 levels of enzyme supplementation). Results showed that chicks fed with germinated barley significantly gained more weight compared to unprocessed barley in all feeding stages (P = 0.0001). Chicks received roasted barley showed a slowdown in their growth so that their weight gain was significantly lower than the control and germinated barley groups in the finishing period and the entire experiment (P = 0.0001). Enzyme supplementation of barley-based diets improved weight gain so that a significant improvement was observed in the growing (P = 0.0002) and throughout the trial (P = 0.0098) over the unsupplemented diets. Chickens fed with germinated barley had a higher length of jejunal villi whereas they had a lower crypt depth compared to untreated or roasted barley groups. As a result, the ratio of villus length to crypt depth as well as villus absorptive surface were significantly higher in birds received germinated barley than those fed with unprocessed or roasted barley. Barley processing resulted in a lower viscosity of digesta in the jejunum so that both processing methods showed a significantly lower viscosity compared to unprocessed barley. Birds received unprocessed barley showed a significantly lower concentration of total cholesterol (P = 0.0370) and LDL-cholesterol (P < 0.01) than those received processed barley. Plasma uric acid (P = 0.0350) and total protein (P = 0.0050) concentrations were significantly greater in birds fed with germinated barley as opposed to other experimental treatments. The mortality from ascites was lower in broilers fed with germinated barley compared to untreated barley, particularly in the absence of NSP-degrading enzyme supplement. In conclusion, germinated barley could significantly improve the performance, gut morphometry, and ascites indices of broiler chickens when compared to untreated barley.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104410"},"PeriodicalIF":3.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular tool for efficient breeding of DOMINANT Greenshell laying hens and significant refinement of phenotypic selection focused on eggshell color","authors":"Kristina Beranova ,&nbsp;Eva Jozova ,&nbsp;Vojtech Anderle ,&nbsp;Michael Rost ,&nbsp;Lukas Zita ,&nbsp;Pavel Beran ,&nbsp;Helena Tyllerova Landova ,&nbsp;Vladislav Curn ,&nbsp;Milan Tyller","doi":"10.1016/j.psj.2024.104425","DOIUrl":"10.1016/j.psj.2024.104425","url":null,"abstract":"<div><div>In recent decades, interest in non-traditionally colored eggs has increased. For breeders, this market interest means breeding lines of laying hens that lay eggs of varied colors, such as the blue-green eggshells (Dominant Greenshell) in this study. This study presents the results of genotyping the polymorphism of the <em>O</em> locus responsible for shell coloration and photometric measurement of eggshell color based on the CIELAb system, which was carried out on the unique Czech breeding population Dominant Greenshell. The aim was to use a combination of phenotyping using the CIELab System method and genotyping of the <em>O</em> locus using the end-point PCR approach with the main focus on the accuracy of distinguishing shell color genotypes, streamlining the selection of dominant homozygotes in the <em>O</em> locus, optimizing this technology for the most efficient and cost-effective selection procedure in practical hen breeding. The optometric method was able to reliably distinguish only dominant and recessive phenotypes and eliminate from the population only undesirable recessive homozygotes with a white colored shell. The parameter a* (redness/greenness) from the CIELab color space turned out to be absolutely key for distinguishing dominant and recessive phenotypes. Using the CART methodology, a classification tree built on discriminating optometric characteristics a-blunt was obtained, however, for the group of desirable <em>O/O</em> homozygotes, the selection approach would result in incorrect genotyping of 31% of individuals. Therefore, a combined approach based on rapid and simple elimination of recessive homozygotes using phenotyping (CIELab photometric measurement) and molecular identification of the EAV-HP insertion in the <em>SLCO1B3</em> gene in dominant phenotypes, regardless of color intensity affected by laying time/order, and allowing reliable elimination, has proven to be the most effective method to distinguish heterozygotes from the breeding population. The combination of optometric and molecular selection methods then leads to more efficient selection, reduction of overall selection costs. This process led to the stabilization of the breeding population within one generation and the achievement of a pure homozygous line with regard to eggshell color.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104425"},"PeriodicalIF":3.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and antibacterial application of peppermint essential oil nanoemulsions in broiler","authors":"Muhammad Jabbar ,&nbsp;Irfan Baboo ,&nbsp;Hamid Majeed ,&nbsp;Zahid Farooq ,&nbsp;Valiollah Palangi","doi":"10.1016/j.psj.2024.104432","DOIUrl":"10.1016/j.psj.2024.104432","url":null,"abstract":"<div><div>In order to mitigate the risk of antibiotic resistance in poultry, scientists nowadays consider plant secondary metabolites to be a major organic antibacterial substitute. This study aimed to characterize and investigate the in silico, <em>in vitro</em>, and <em>in vivo</em> antibacterial effects of peppermint essential oil (PEO) in the form of a nanoemulsion (NE), termed PEONE. Menthol as a major compound of PEO has been identified by gas chromatography and mass spectroscopy (GCMS) analysis as 32.3 %, while lower droplet size, polydispersity Index (PDI), and optimum zeta potential values depicted the stability of PEONE have been observed and validated by transmission electron microscopy (TEM) micrograph image. In silico antibacterial activity was studied by molecular docking of menthol and enrofloxacin with Topoisomerase IV protein (PDB: <span><span>1s16</span><svg><path></path></svg></span>;) of <em>Escherichia coli</em> K12 MG1655 and this effect was validated by <em>in vitro</em> and <em>in vivo</em> analysis. <em>In vitro</em> analysis, sustained release of PEONE has been observed against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. In this study for <em>in vivo</em> experiments (<em>n</em> = 90) day-old broiler chicks were distributed into 6 dietary treatments with 5 replicates of 3 birds per replication. Dietary treatments included 1) Negative control (basal diet), 2) Positive control (basal diet + 200 µl enrofloxacin), 3) 25 µl PEONE + basal diet, 4) 50 µl PEONE + basal diet, 5) 75 µl PEONE + basal diet, and 6) 100 µl PEONE + basal diet. Analyzed data by different statistical tools confirmed that PEONE significantly affected body weight gain (BWG) with an improved feed conversion ratio (FCR) compared to the control group. A significant increase in cecal <em>Lactobacillus</em> count and a decrease in total coliform was observed. Positive effects on physiological parameters, visceral organs, and meat quality characteristics have been observed. In conclusion, our experiments suggest that PEONE can be used in the broiler industry as a substitute for antibiotics to minimize bacterial resistance.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104432"},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypericum perforatum L. (St. John's Wort) in broilers diet improve growth performance, intestinal microflora and immunity.","authors":"Ziya İlhan, Muhittin Zengin, Oğuz Koray Bacaksız, Ergün Demir, İsmail Hakkı Ekin, Mehmet Ali Azman","doi":"10.1016/j.psj.2024.104419","DOIUrl":"10.1016/j.psj.2024.104419","url":null,"abstract":"<p><p>Hypericum perforatum L. (St. John's Wort) extract (HPE), powdered H. perforatum (PHP), and selenium (Se) on growth, intestinal flora, and immunity of broiler chicks were investigated. In total, 504 one-day-old broiler chicks were randomly allocated into 6 dietary treatments, which were then denoted as negative control (NC) group (basal diet), containing organic Se 0.2% in the starter and grower period as positive control (PC), containing 1% PHP in the starter and grower period, and HPE I, HPE II, and HPE III groups containing respectively, 1.5, 3.0, and 4.5 mL / kg HPE in the starter and grower period. The results on performance showed that a significant (P < 0.05) higher body weight of chickens in the HPE III group was observed when compared with that of the NC and PHP groups. Although average daily weight gain and feed intake are significant in the HPE III group, the difference in terms of total feed conversion rate was insignificant (P > 0.05). The liver weights in PC and HPE III were lower compared to HPE I (P < 0.05). The difference in total lactic acid bacteria count (TLABC) between the NC group and all HPE groups was found to be significant (P ˂ 0.05), in addition to TLABC was higher in the HPE III group than other groups (P = 0.001). The highest serum antibody titers to the Newcastle disease vaccine were determined in the HPE III group on the 24th, 35th, and 42nd days of age. IL-1B and IL-6 were found to be insignificant between the groups in chickens (P ˃ 0.05). TNF-α in the HPE III group was greatly increased than the other groups and significant compared to the NC and HPE I groups (P = 0.018). In conclusion, 4.5 mL / kg HPE, which has a low production cost and is easy to extract and without causing environmental problems, varied significantly in their impact on growth performance, intestinal microflora, and immunity of growing broilers.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104419"},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability","authors":"Ferdinand G. Tchodo ,&nbsp;Hervé B. Dakpogan ,&nbsp;Benjamin Adjei-Mensah ,&nbsp;Ombortime N'nanle ,&nbsp;Simplice Karou ,&nbsp;Wéré Pitala ,&nbsp;Kokou Tona ,&nbsp;Batomayena Bakoma","doi":"10.1016/j.psj.2024.104435","DOIUrl":"10.1016/j.psj.2024.104435","url":null,"abstract":"<div><div>TOXICO-pathological effects of herbal plants have always been a major concern, but little information is provided on the toxico-pathological effects of medicinal plants used against coccidiosis. This study aimed to assess the histopathological effects of <em>Carica papaya</em> seeds (CPS), <em>Azadirachta indica</em> leaves (AIL), and <em>Sarcocephalus latifolius</em> root (SLR) used as coccidiostat in traditional poultry farms with various doses using the chick embryo model. A total of 420 Sasso breeder eggs at ED4 of incubation were inoculated with the extract of these plants following a 3×4 factorial design with 3 plant extracts (CPS, AIL and SLR) and 4 inoculation doses (0, 25, 50 and 100 mg/kg egg-weight). From ED6 to ED19, the weights of the albumen and embryo were recorded, and the weights of ED19 embryo organs such as liver, heart, kidney, and lungs were measured. Additionally, histopathological lesions were examined. The results indicated that the presence of various phytoconstituents such as alkaloids, phenolics, flavonoids, tannins, saponins, coumarins, steroids, and triterpenes with statistically significant free-radical-scavenging ability differed among the plant extracts (p &lt; 0.0001). Toxico-pathological examination revealed a dose-dependent slight toxicity (p &lt; 0.0001) of the <em>Azadirachta indica</em> leaves extract compared to the other plants. Additionally, the relative organ weight showed kidney hypertrophy (p = 0.001) and liver hypertrophy (p = 0.0001), as well as dilation of hepatic and cardiac vessels. The conclusion drawn was that chicken embryos are more susceptible to <em>in ovo</em> inoculation with <em>Azadirachta indica</em> leaves compared to <em>Carica papaya</em> seeds and <em>Sarcocephalus latifolius</em> root.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104435"},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens","authors":"Zewei Li ,&nbsp;Yifei Chen ,&nbsp;Haiping Xie ,&nbsp;Quan Li ,&nbsp;Shifeng Wang ,&nbsp;Huoying Shi","doi":"10.1016/j.psj.2024.104436","DOIUrl":"10.1016/j.psj.2024.104436","url":null,"abstract":"<div><div>Necrotic enteritis (NE) caused by <em>Clostridium perfringens</em> (<em>C. perfringens</em>) has resulted in significant losses for the poultry industry worldwide. Currently, there is no widely promoted vaccine for NE. In this study, immunoprecipitation (IP) was employed to isolate immunogenic proteins of <em>C. perfringens</em>, and 118 potential candidate antigens were identified through liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS). From these, three candidate antigen proteins were selected based on their predicted antigenicity, hydrophilicity, stability, and transmembrane signalling properties: ArcB (an ornithine aminotransferase), TmpC (a probable membrane lipoprotein), and EntB (a possible enterotoxin). These three proteins were successfully produced in large quantities using <em>Escherichia coli</em> (<em>E. coli</em>), with confirmed good solubility. Both in <em>vitro</em> and <em>in vivo</em> research demonstrated that these antigens possess strong immunogenicity, eliciting robust antigen-specific humoral and cellular immune responses in chickens and mitigating NE symptoms caused by <em>C. perfringens</em>. The candidate antigens identified through immunoproteomics hold potential as subunit vaccines against <em>C. perfringens</em> infection.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104436"},"PeriodicalIF":3.8,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocatechuic acid attenuates intestinal inflammation through TLR4/p38 and NF-κB pathways in heat-stressed broilers","authors":"Shuaiyong Wang ,&nbsp;Xin Wang ,&nbsp;Yu Gao ,&nbsp;Yuchen Fu ,&nbsp;Zhaoqing Han ,&nbsp;Ping Xu ,&nbsp;Jianli Tang","doi":"10.1016/j.psj.2024.104424","DOIUrl":"10.1016/j.psj.2024.104424","url":null,"abstract":"<div><div>Protocatechuic acid (<strong>PCA</strong>), a major active component found in <em>Eucommia ulmoides Oliver</em>, is involved in regulating oxidative stress and inflammation. Heat stress poses a significant threat to the poultry industry. In this study, we investigated the protective effect of PCA on intestinal homeostasis under heat stress. Our results indicated that PCA improved the growth performance of broilers during heat stress. Furthermore, PCA mitigated jejunal damage and attenuated the upregulation of inflammatory mediators, including TNF-α, IL-6, and IL-1β, triggered by heat stress. Concurrently, it restored the activity of superoxide dismutase (<strong>SOD</strong>) and total antioxidant capacity (<strong>T-AOC</strong>). Furthermore, PCA maintained the jejunum function by increasing the levels of ZO-1, Claudin-1, and Occludin. Mechanistically, PCA inhibited the activation of TLR4/p38 MAPK and NF-κB pathways, thereby regulating the imbalance in oxidative stress and inflammatory responses caused by heat stress.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104424"},"PeriodicalIF":3.8,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}