Poultry Science最新文献

{"title":"Oregano-based feed additive reduces Salmonella enterica serovar Enteritidis colonization in young broilers","authors":"Christina L. Swaggerty ,&nbsp;M. Dolores Cabrera ,&nbsp;Santiago Sasia ,&nbsp;Gabriel Cabrera ,&nbsp;Kenneth J. Genovese ,&nbsp;J. Allen Byrd II ,&nbsp;Michael H. Kogut ,&nbsp;Rafael A. Cabrera","doi":"10.1016/j.psj.2025.105301","DOIUrl":"10.1016/j.psj.2025.105301","url":null,"abstract":"<div><div><em>Salmonella</em> is one of the leading bacterial foodborne pathogens and is commonly found in poultry. The objective of this study was to determine if feeding an oregano-based antibiotic alternative could reduce the burden of <em>Salmonella</em> in broilers. Day-of-hatch straight-run broiler chicks were provided a basal corn-soymeal diet. After four days on the basal diet, birds were assigned to a dietary treatment group (<em>n</em> = 50 chicks/group/experiment). The groups were control with no oregano feed additive or a diet supplemented with an oregano oil product (300 g/ton). At 7-days-of-age, chicks were challenged via oral gavage (0.5 mL) with <em>Salmonella enterica</em> serovar Enteritidis (1 × 10^4-5 colony forming units [cfu]/chick). Fourteen-days post-challenge (dpc), 50 birds from each group were euthanized. At necropsy, the liver and one cecal pouch were removed and enriched for <em>S</em>. Enteritidis and cecal content was analyzed for <em>S</em>. Enteritidis enumeration. The study was conducted twice. Data were analyzed to determine differences in <em>S</em>. Enteritidis recovered between the groups with <em>P</em> &lt; 0.05 significant. No differences were observed in the percent positive liver samples in the controls compared to those on the oregano diet. There was a reduction (<em>P</em> = 0.003) in the positive ceca between controls (61 %) compared to chickens on the oregano diet (40 %). The <em>S</em>. Enteritidis recovered from cecal content was also reduced (<em>P</em> &lt; 0.001) in chickens on the oregano diet (3.2 × 10³ cfu/g) compared to controls (3.5 × 10⁵ cfu/g). Cytokine (IL-1β, IL-18) and chemokine (CXCL8) mRNA expression was also 1.9-2.2-fold higher (<em>P</em> ≤ 0.04) in the cecal tonsil of chickens on the oregano diet compared to controls indicating immunomodulatory affect. Collectively, these data demonstrate addition of an oregano-based feed additive to a broiler diet reduces <em>S</em>. Enteritidis colonization and invasion in the ceca and may be an important pre-harvest intervention strategy to reduce the burden of this highly important foodborne pathogen.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105301"},"PeriodicalIF":3.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary grape pomace ameliorates intestinal damage and oxidative stress by modulating MAPK-Nrf2/ARE pathways in coccidia-challenged broilers (Gallus gallus)","authors":"Milan K. Sharma,&nbsp;Eliot M. Dugan,&nbsp;Melissa Y. Huang,&nbsp;Cydney Jackson,&nbsp;Matthew J. Pataki,&nbsp;Peter R. Gracey,&nbsp;Chloe J. McGovern,&nbsp;Elad Tako","doi":"10.1016/j.psj.2025.105364","DOIUrl":"10.1016/j.psj.2025.105364","url":null,"abstract":"<div><div>Grape pomace (<strong>GP</strong>) is an agricultural byproduct rich in bioactive compounds with antimicrobial, antioxidant, and immunomodulatory properties that have proven to have strong antioxidant and immunomodulatory properties in chickens. As a part of a defense mechanism, coccidiosis initiates oxidative bursts, generating large amounts of free radical species that negatively affect the gut health of chickens. This study aimed to evaluate the effect of dietary inclusion of GP in broilers infected with coccidiosis on physiological and molecular markers of gut health, immune response, and oxidative status. A total of 120 male broiler chickens were randomly assigned to one of the four treatments. The four treatment groups were i) non-challenged control without any dietary feed additives (<strong>NCC</strong>), ii) challenged control without any dietary feed additives (<strong>CC</strong>), iii) CC with 0.5 % dietary inclusion of concord grape pomace (<strong>0.5GP</strong>), and iv) CC with 0.75 % dietary inclusion of concord grape pomace (<strong>0.75GP</strong>). On d 14, broilers grouped in CC, 0.5GP, and 0.75GP were orally inoculated with 20 × doses of commercial live coccidiosis vaccine to induce coccidiosis. Results indicated that 0.75 % inclusion of GP reduced the occurrence and severity of the intestinal lesions associated with <em>Eimeria</em> at d 20 (<em>P</em>&lt;0.05). The inclusion of either 0.5 or 0.75 % of GP improved the intestinal histomorphology in all three sections of the small intestine during the acute and recovery phases of coccidial infection (<em>P</em>&lt;0.05). At d 20, 0.75GP tended to maintain the same level of IL-1β as that of NCC and downregulated the expression of IL-10 (<em>P</em>&lt;0.1). Additionally, during the recovery phase, the CD8+ T-cell population was lower in the 0.75GP group compared to CC and 0.5GP (<em>P</em>=0.059). At d 20, the MAPK-Nrf2/ARE pathway was activated due to oxidative burst by coccidial challenge; however, 0.75 % GP inclusion was able to inactivate the path (<em>P</em>&lt;0.05). These findings suggest that grape pomace possesses the potential as a sustainable feed additive to improve gut health, immune response, and oxidative stability in broilers infected with coccidiosis.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105364"},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics and lipidomics analysis of the breast muscles to investigate the effect of dietary copper level on the meat quality of pigeons","authors":"Bo Zhang,&nbsp;Wenli Liu,&nbsp;Li Shen,&nbsp;Yusheng Gao,&nbsp;Yuxin Shao,&nbsp;Yipu Li,&nbsp;Yangyang Wang,&nbsp;Dongdong Zhao,&nbsp;Jing Li,&nbsp;Zheng Wang","doi":"10.1016/j.psj.2025.105352","DOIUrl":"10.1016/j.psj.2025.105352","url":null,"abstract":"<div><div>Pigeon meat, known for its high nutritional value and tender texture, can be influenced by various dietary factors. Copper, an essential trace mineral, plays a vital role in growth, development, and overall meat quality. However, its impact on pigeon meat has not been well studied. This study evaluated the effects of dietary copper supplementation on production performance, carcass traits, meat quality, fatty acid composition, and protein profiles in squabs. A total of 120 pairs of 60-week-old White King pigeon breeders (one male and one female per pair) were randomly assigned to five dietary treatment groups, each receiving diets containing 5.66, 9.66, 13.66, 17.66, or 21.66 mg/kg of copper. Feeding by parent pigeons, the squabs were reared to 28 days of age and then used for sampling and analyses. The result showed that copper deficiency led to increased lipid accumulation in the breast muscle, raising levels of total lipids, triglycerides, cholesterol, and free fatty acids, which impaired shear force and meat texture. Copper supplementation reduced saturated fatty acids and increased beneficial ω-3 polyunsaturated fatty acids (methyl linolenate). Proteomic analysis revealed that copper enhanced tubulin expression, which is crucial for muscle cell integrity, and influenced enzymes involved in fatty acid metabolism. Regional variation in copper supplementation highlighted the importance of optimizing copper levels in pigeon diets. Statistical analyses included one-way ANOVA and quadratic regression analysis, which identified the optimal dietary copper level for improving meat quality as 17.2∼18.4 mg/kg. These findings suggest that adequate copper supplementation can significantly improve both the texture and nutritional quality of pigeon meat, providing valuable insights into the pigeon industry and consumer health.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105352"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technical note: Sustainable snail meal enhances duck egg production and health","authors":"Yunqian He ,&nbsp;Saikun Pan ,&nbsp;Shengjun Wu","doi":"10.1016/j.psj.2025.105353","DOIUrl":"10.1016/j.psj.2025.105353","url":null,"abstract":"<div><div>This study evaluated the effects of replacing soybean meal with snail meal on laying performance, egg quality, and blood parameters in laying ducks. Five experimental diets with graded levels of soybean meal replacement by snail meal were formulated: T0 (0% replacement), T1 (25% replacement), T2 (50% replacement), T3 (75% replacement), and T4 (100% replacement). Ducks fed the T2 (50% replacement) diet showed the highest egg production rate (87%), average egg weight (68.0 g), and egg mass (59.2 g/day), along with the lowest feed conversion ratio (2.18). Egg quality also improved, with increased yolk color score (10.0), Haugh unit (86.0), and eggshell strength (4.1 kg/cm²). Serum analysis revealed higher superoxide dismutase (SOD) (85.48 U/mL), glutathione peroxidase (GPX) (148.59 U/mL), and IgG (2.34 g/L) levels in the 50% group, indicating enhanced antioxidant and immune functions. These findings suggest that replacing 50% of soybean meal with snail meal optimizes laying performance and physiological health in ducks, and offers a promising strategy for sustainable protein substitution in poultry diets.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105353"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary phytogenic inclusion level affects performance and expression of heat shock, cytoprotective, inflammatory and apoptotic genes in the duodenum and the liver of cyclic heat-challenged broilers","authors":"Eugenia Iliopoulou ,&nbsp;Ioannis Brouklogiannis ,&nbsp;Vasileios V. Paraskeuas ,&nbsp;Eirini Griela ,&nbsp;Evangelos C. Anagnostopoulos ,&nbsp;George Kefalas ,&nbsp;Konstantinos C. Mountzouris","doi":"10.1016/j.psj.2025.105348","DOIUrl":"10.1016/j.psj.2025.105348","url":null,"abstract":"<div><div>This study was conducted to investigate the inclusion level effect of a phytogenic blend (PB) on performance and the expression of genes relevant to heat shock response, detoxification, antioxidant capacity, inflammation and apoptosis in the duodenum and the liver of broilers under heat challenge conditions. The PB consisted of bioactive substances derived from oregano, thyme and olive oil polyphenols. Depending on PB inclusion level (i.e., 0, 500, 1,000, 1,500 and 2000 mg/kg diet) in the basal diet, 490 male broilers Ross 308, 1-<span>d</span>-old were assigned into 5 treatments: CON, PB500, PB1000, PB1500, and PB2000, with 7 replicates of 14 broilers, each. Birds were reared for 35 days under thermoneutral conditions and then subjected to 7 h cyclic heat stress (32 ± 1°C; RH 55 ± 5 %) for 5 consecutive days. Performance responses were closely monitored per growth phase (starter 1-10d, grower 11-24, finisher 25-40) and overall. At the end of the experiment (d40), duodenal and liver samples from 7 broilers per treatment were appropriately collected, snap frozen and subsequently stored at -80°C until gene expression analysis. Results revealed that PB inclusion level, improved (<em>P</em> &lt; 0.05) Feed Conversion Ratio, Protein Efficiency Ratio, Energy Efficiency Ratio and the Nutritional Efficiency in a quadratic manner, compared to CON. At the molecular level, PB inclusion down-regulated (<em>P</em> &lt; 0.05) most of the heat shock and inflammation-related genes, both in the duodenum and liver, in a linear manner with PB inclusion level. In addition, PΒ cytoprotective capacity was demonstrated via beneficial changes (<em>P</em> &lt; 0.05) seen for the majority of the antioxidant and detoxification-related genes assessed, with the PB1500 displaying most significant differences, compared to CON. Conclusively, in heat challenged broilers, the multi-pathway nutrigenomic analysis provided new mechanistic support behind the improved broiler performance responses with PB inclusion.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105348"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune responses and recovery from spotty liver disease in layer birds","authors":"Sarah Eastwood ,&nbsp;Timothy B. Wilson ,&nbsp;Jiongrui Huang ,&nbsp;Bronwyn E. Campbell ,&nbsp;Peter C. Scott ,&nbsp;Robert J. Moore ,&nbsp;Thi Thu Hao Van","doi":"10.1016/j.psj.2025.105351","DOIUrl":"10.1016/j.psj.2025.105351","url":null,"abstract":"<div><div>Spotty Liver Disease (SLD), caused by <em>Campylobacter hepaticus</em>, greatly impacts the health and egg production of affected layer hens and is a disease of concern in the poultry industry. This study aimed to enhance the understanding of the immune response in chickens to <em>C. hepaticus</em> infection and their ability to resist reinfections. One hundred- and twenty-layer chickens were allocated to 10 groups and challenged, from one to three times, with <em>C. hepaticus</em> HV10^T, with six weeks between each challenge. Blood and cloacal swabs were collected every three weeks to assess antibody responses and <em>Campylobacter</em> presence. Upon necropsy, bile, spleen, jejunum, and blood samples were collected for <em>C. hepaticus</em> detection and host gene expression analysis using qPCR and RNA sequencing. We found that most birds challenged with <em>C. hepaticus</em> for the second or third time did not develop liver lesions even with the presence of <em>C. hepaticus</em> in their bile, suggesting that birds were resistant to disease development following repeated exposure. Anti-<em>C. hepaticus</em> antibodies increased significantly six weeks after a single challenge but reduced from nine weeks. Quantitative PCR demonstrated that <em>C. hepaticus</em> could be recovered from the bile six weeks after a single challenge and increased significantly after a secondary challenge. RNA-seq and qPCR data demonstrate an elevation in pro-inflammatory cytokines, such as interleukin 6 (IL-6) and interleukin 1ß (IL-1ß), after a secondary challenge and down-regulation during a third challenge. Expression of many genes encoding barrier-supporting proteins genes were differentially expressed, with increased expression following a third challenge compared to expression after a single challenge. Comparison of gene expression in tissues of triple to a single challenged birds demonstrated that many genes involved in cytokine activity and the JAK-STAT cascade were down-regulated whereas other immune system pathways were up-regulated. Altogether, the results indicate that over time, immune memory, enhanced barrier function, and a balanced immune response developed, resulting in reduced impact of infection on the birds. These findings show that the impact of <em>C. hepaticus</em> infection can be ameliorated by immune responses and hence indicate that vaccines that induce appropriate protective immune responses should provide an effective tool to reduce SLD in poultry.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105351"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of early feeding technologies providing methionine supplementation on performance, lipid oxidation, and some immune-related gene expression in broiler chicken","authors":"Virág Ács ,&nbsp;Nóra Szeli ,&nbsp;József Nagy ,&nbsp;Szilvia Áprily ,&nbsp;Annamária Tischler ,&nbsp;Orsolya Csötönyi ,&nbsp;Ildikó Jócsák ,&nbsp;Ildikó Benedek ,&nbsp;Örs Petneházy ,&nbsp;Janka Turbók ,&nbsp;Judit Enyezdi ,&nbsp;Veronika Halas","doi":"10.1016/j.psj.2025.105335","DOIUrl":"10.1016/j.psj.2025.105335","url":null,"abstract":"<div><div><em>In ovo</em> administration of DL-methionine and post-hatch Hydrogel® supplements were tested to examine the impact of early feeding on performance and immune-related traits in a commercial broiler stock. One thousand one hundred and twenty Ross 308 eggs were incubated and assigned to seven treatment groups: intact (no <em>in ovo</em> administration) and immediate feed access (<strong>C1</strong>), <em>in ovo</em> saline treatment and immediate feed access (<strong>C2)</strong>, intact and delayed feeding (<strong>ID</strong>), <em>in ovo</em> saline treatment and delayed feeding (<strong>IoS</strong>), <em>in ovo</em> DL-Methionine treatment and delayed feeding (<strong>IoM</strong>), intact and delayed access to feed, but immediate access to commercial Hydrogel® without (<strong>Hyd</strong>) or with 5mg/kg (<strong>HydM</strong>) DL-methionine post-hatch. The results showed, that the <em>in-ovo</em> methionine may have positive effects on the weight gain of the birds (<em>p</em> &lt; 0.001) compared to the commercial Hydrogel® however, it cannot compete with the immediate feeding. The number of heterophils decreased significantly (<em>p</em> &lt; 0.001) by day 21 in <strong>ID</strong> and <strong>IoS</strong> compared to the immediately fed control (<strong>C1</strong>). The number of lymphocytes, monocytes, and eosinophils, increased in treatments supplemented with methionine (<em>p</em> &lt; 0.05) <em>(</em><strong><em>IoM</em></strong><em>,</em> <strong><em>HydM)</em></strong> indicating enhanced immune protection.</div><div>There were no differences in the total antioxidant capacity (<strong>FRAP</strong>) and malonaldehyde concentration (<strong>MDA</strong>) (<em>p</em> = 0.07) in the examined groups. The Cytochrome P450 H1 (<strong>CYP2H1</strong>) gene was downregulated in all treatment groups (on days 21 and 35) indicating a slower metabolism, particularly in the ID group compared to C1 and C2 (<em>p</em> &lt; 0.001). The <strong>HydM</strong> treatment could upregulate the <strong>IL2</strong> expression as the immediate feeding, while only <strong>IoM</strong> treatment resulted in significant downregulation by day 35 (<em>p</em> &lt; 0.001). <strong>IL6</strong> was upregulated in all treatment groups (<em>p</em> &lt; 0.001) except for <strong>HydM,</strong> where the gene expression did not differ from the housekeeping gene. Early administration of dietary methionine has a positive effect on performance and the immune system, however, none of the early feeding methods can compete with immediate feed access. The possible positive effects of early nutrition and its epigenetic impact should be examined in further studies.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105335"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adrenergic pathways in glycine-mediated feeding behavior: Evidence from layer chickens","authors":"Hamed Zarei ,&nbsp;Fatemeh Kashefi ,&nbsp;Keyvan Hasani","doi":"10.1016/j.psj.2025.105338","DOIUrl":"10.1016/j.psj.2025.105338","url":null,"abstract":"<div><div>Glycinergic and adrenergic systems are integral to the regulation of meal consumption in avian species; however, the interactions between these systems have not been previously documented. This investigation was conducted to explore the interplay between the central glycinergic and adrenergic systems in regulating feed consumption among egg-type chickens. To this end, six separate experiments were performed. The first experiment involved intracerebroventricular (ICV) infusion of normal saline and glycine at different doses (50, 100, and 200 nmol) to determine its effective dose. In experiments 2-6, sub-effective doses of various adrenergic receptor antagonists—prazosin (an antagonist of α1 receptor), yohimbine (an antagonist of α2 receptor), metoprolol (an antagonist of β1 receptor), ICI 118.551 (an antagonist of β2 receptor), and SR 59230R (an antagonist of β3 receptor)—were co-administered with an effective dose of glycine to assess the potential involvement of adrenergic receptors in glycine-mediated feed intake. The cumulative feed consumption was subsequently measured at intervals of 30, 60, and 120 minutes post-administration. The findings indicated that glycine infusion at doses of 100 and 200 nmol significantly reduced meal consumption in layer chickens compared to the saline-treated control group (<em>P</em> &lt; 0.05). Additionally, co-infusion of a β2 receptor antagonist attenuated the hypophagic effects typically induced by glycine (<em>P</em> &lt; 0.05). However, co-administration of α1, α2, β1, and β3 receptor antagonists did not alter glycine-induced hypophagia (<em>P</em> ≥ 0.05). These findings suggest that the role of glycine in decreasing feed consumption in layer chickens could be facilitated by β2 adrenergic receptors<strong>.</strong></div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105338"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of external morphological malformations induced by hyperthermia exposure during the blastula stage in an ex-ovo (shell-less) culture of Gallus gallus domesticus embryos","authors":"Osvaldo Macias-Marin ,&nbsp;Alma L. Guerrero-Barrera ,&nbsp;Arturo G. Valdivia-Flores ,&nbsp;Teodulo Quezada-Tristan ,&nbsp;Flor Y. Ramirez-Castillo ,&nbsp;Adriana C. Moreno-Flores ,&nbsp;Fabiola Galindo-Guerrero ,&nbsp;Francisco J. Avelar-Gonzalez ,&nbsp;Adriana D. Rodriguez-Padilla","doi":"10.1016/j.psj.2025.105341","DOIUrl":"10.1016/j.psj.2025.105341","url":null,"abstract":"<div><div>The chicken is a valuable model to study embryonic development, genetic manipulation, regenerative medicine, tumorigenesis, vaccine development, toxicology, and teratology research. Different methods have been described for incubating chicken embryos outside the eggshell (ex-ovo or shell-less incubation). To date, no studies have focused on using ex-ovo incubation as a model to study the effects of hyperthermia as a teratogen. In this work, a total of 350 fertile chicken eggs were used to study the development of congenital malformations in an ex-ovo and in-ovo incubation system exposed to normal (37.5°C) and hyperthermia conditions (40°C). In the ex-ovo hyperthermia test, all ex-ovo embryos (<em>n</em> = 50) developed malformations; only 0.02 % reached 5 days of development. In the ex-ovo normal temperature group, none of the ex-ovo embryos developed malformations, and 48 % reached 21 days of development; there was no significant morphological difference between the ex-ovo normal temperature and the in-ovo normal temperature group. The time of exposure to hyperthermia conditions is crucial for the development of malformations, with the blastula stage (0 h) being the most susceptible. Blastula stage malformations, neural tube defects, microphthalmia, amelia, gastroschisis, caudal regression, and the development of twinning were identified. The ex-ovo incubation system does not increase the risk of the development of malformations, and it is a viable model for studying the effects of teratogens, as well as the morphology and physiology of the embryo.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105341"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duck plague virus US3 kinase phosphorylates and induces STING degradation to inhibit innate immune responses","authors":"Bin Tian ,&nbsp;Yanming Tian ,&nbsp;Dongjie Cai ,&nbsp;Huanhuan Cao ,&nbsp;Liping Wu ,&nbsp;Mingshu Wang ,&nbsp;Qiao Yang ,&nbsp;Ying Wu ,&nbsp;Renyong Jia ,&nbsp;Dekang Zhu ,&nbsp;Mafeng Liu ,&nbsp;Shun Chen ,&nbsp;Xinxin Zhao ,&nbsp;Shaqiu Zhang ,&nbsp;Juan Huang ,&nbsp;Di Sun ,&nbsp;Xumin Ou ,&nbsp;Zhen Wu ,&nbsp;Anchun Cheng","doi":"10.1016/j.psj.2025.105336","DOIUrl":"10.1016/j.psj.2025.105336","url":null,"abstract":"<div><div>Duck plague virus (DPV) causes the highest mortality rate among aquatic birds; however, its antago nistic mechanism against antiviral innate immune responses remains elusive. In this study, we systematically screened and found that most DPV genes have inhibitory potential for duck cyclic guanosine monophosphate-adenosine monophosphate synthetase (cGAS)/stimulator of interferon (IFN) gene (STING) pathway-mediated antiviral responses, with the DPV US3 kinase showing the strongest inhibitory activity. Co-immunoprecipitation and immunoblotting assays demonstrated that DPV US3 interacted with STING and induced its degradation. Further mutagenesis experiments revealed that DPV US3 kinase activity was essential for phosphorylating STING, reducing STING dimerization, and inhibiting STING-mediated antiviral responses. Sequence alignment and mutagenesis studies have demonstrated that DPV US3 phosphorylates STING at serine 86, near the Endoplasmic reticulum (ER) retention sequence (R₈₂YRGS₈₆), disrupting its association with tank-binding kinase 1 (TBK1) and inducing STING degradation. Finally, US3 knockout attenuated DPV replication by activating higher levels of IFN and ISGs <em>in vitro</em> and <em>in vivo</em>. These results demonstrate that DPV promotes viral infection and pathogenicity by inducing STING degradation through the encoded US3 kinase, providing new insights into the mechanism of DPV immune evasion.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 8","pages":"Article 105336"},"PeriodicalIF":3.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}