{"title":"山东省食用动物沙门氏菌发生及分子特征调查。","authors":"Fengzhi Liu, Peng Duan, Huaping Xiao, Hui Zhang, Hongmei Guo, Ruihua Zhang, Shijin Jiang","doi":"10.1016/j.psj.2025.105628","DOIUrl":null,"url":null,"abstract":"<p><p>Salmonella is a significant zoonotic pathogen affecting both humans and animals. Between April 2023 and May 2024, we obtained 124 Salmonella strains from 1,413 samples (8.78 %) collected from food animals in Weifang, Zibo, Heze, Taian, Linyi, Binzhou, and Jining regions of Shandong Province, China. This study aimed to analyze serovar distribution, multilocus sequence typing (MLST), antimicrobial susceptibility, and plasmid typing of isolated strains. The 124 Salmonella isolates were serotyped into 5 distinct serovars, and S. Typhimurium (58/124, 46.77 %) was the predominant serotype. MLST revealed six distinct sequence types, among which ST19 (40/124, 32.26 %) was the most common. Of the 124 isolates, 83 (66.94 %) displayed multidrug resistance, with ampicillin resistance (88/124, 70.97 %) being the most prevalent phenotype. The bla<sub>TEM</sub> gene (57/124, 45.97 %) was the most common resistance gene, followed by bla<sub>CTX-M</sub> (45/124, 36.29 %), qnrS (39/124, 31.45 %), aph(3')-II (36/124, 29.03 %), oqxA (29/124, 23.39 %), and oqxB (28/124, 22.58 %). These Salmonella strains carried a total of six plasmid incompatibility groups, with IncFII (35/124, 28.23 %) being the most prevalent. Conjugation experiments demonstrated successful transfer of the bla<sub>TEM</sub> gene from 36 donors (36/57, 63.16 %) to Escherichia coli J53. The spread of resistance genes is largely driven by antibiotic misuse. In conclusion, controlling Salmonella prevalence and limiting antibiotic use in pigs and ducks are crucial for safeguarding public health.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105628"},"PeriodicalIF":4.2000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356472/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigation into the occurrence and molecular characteristics of Salmonella from food animals in Shandong, China.\",\"authors\":\"Fengzhi Liu, Peng Duan, Huaping Xiao, Hui Zhang, Hongmei Guo, Ruihua Zhang, Shijin Jiang\",\"doi\":\"10.1016/j.psj.2025.105628\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Salmonella is a significant zoonotic pathogen affecting both humans and animals. Between April 2023 and May 2024, we obtained 124 Salmonella strains from 1,413 samples (8.78 %) collected from food animals in Weifang, Zibo, Heze, Taian, Linyi, Binzhou, and Jining regions of Shandong Province, China. This study aimed to analyze serovar distribution, multilocus sequence typing (MLST), antimicrobial susceptibility, and plasmid typing of isolated strains. The 124 Salmonella isolates were serotyped into 5 distinct serovars, and S. Typhimurium (58/124, 46.77 %) was the predominant serotype. MLST revealed six distinct sequence types, among which ST19 (40/124, 32.26 %) was the most common. Of the 124 isolates, 83 (66.94 %) displayed multidrug resistance, with ampicillin resistance (88/124, 70.97 %) being the most prevalent phenotype. The bla<sub>TEM</sub> gene (57/124, 45.97 %) was the most common resistance gene, followed by bla<sub>CTX-M</sub> (45/124, 36.29 %), qnrS (39/124, 31.45 %), aph(3')-II (36/124, 29.03 %), oqxA (29/124, 23.39 %), and oqxB (28/124, 22.58 %). These Salmonella strains carried a total of six plasmid incompatibility groups, with IncFII (35/124, 28.23 %) being the most prevalent. Conjugation experiments demonstrated successful transfer of the bla<sub>TEM</sub> gene from 36 donors (36/57, 63.16 %) to Escherichia coli J53. The spread of resistance genes is largely driven by antibiotic misuse. In conclusion, controlling Salmonella prevalence and limiting antibiotic use in pigs and ducks are crucial for safeguarding public health.</p>\",\"PeriodicalId\":20459,\"journal\":{\"name\":\"Poultry Science\",\"volume\":\"104 10\",\"pages\":\"105628\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356472/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.psj.2025.105628\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.psj.2025.105628","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/5 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Investigation into the occurrence and molecular characteristics of Salmonella from food animals in Shandong, China.
Salmonella is a significant zoonotic pathogen affecting both humans and animals. Between April 2023 and May 2024, we obtained 124 Salmonella strains from 1,413 samples (8.78 %) collected from food animals in Weifang, Zibo, Heze, Taian, Linyi, Binzhou, and Jining regions of Shandong Province, China. This study aimed to analyze serovar distribution, multilocus sequence typing (MLST), antimicrobial susceptibility, and plasmid typing of isolated strains. The 124 Salmonella isolates were serotyped into 5 distinct serovars, and S. Typhimurium (58/124, 46.77 %) was the predominant serotype. MLST revealed six distinct sequence types, among which ST19 (40/124, 32.26 %) was the most common. Of the 124 isolates, 83 (66.94 %) displayed multidrug resistance, with ampicillin resistance (88/124, 70.97 %) being the most prevalent phenotype. The blaTEM gene (57/124, 45.97 %) was the most common resistance gene, followed by blaCTX-M (45/124, 36.29 %), qnrS (39/124, 31.45 %), aph(3')-II (36/124, 29.03 %), oqxA (29/124, 23.39 %), and oqxB (28/124, 22.58 %). These Salmonella strains carried a total of six plasmid incompatibility groups, with IncFII (35/124, 28.23 %) being the most prevalent. Conjugation experiments demonstrated successful transfer of the blaTEM gene from 36 donors (36/57, 63.16 %) to Escherichia coli J53. The spread of resistance genes is largely driven by antibiotic misuse. In conclusion, controlling Salmonella prevalence and limiting antibiotic use in pigs and ducks are crucial for safeguarding public health.
期刊介绍：
First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers.
An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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