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The influence of relative humidity during the first 21 days post-hatch on the production performance, biochemical indices, and meat quality of Pekin ducks. 孵化后前 21 天相对湿度对北京鸭生产性能、生化指标和肉质的影响
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-31 DOI: 10.1016/j.psj.2024.104473
Dongyue Sun, Congcong Xu, Yi Liu, Zichun Dai, Ziyi Pan, Rong Chen, Rihong Guo, Fang Chen, Zhendan Shi, Shijia Ying
{"title":"The influence of relative humidity during the first 21 days post-hatch on the production performance, biochemical indices, and meat quality of Pekin ducks.","authors":"Dongyue Sun, Congcong Xu, Yi Liu, Zichun Dai, Ziyi Pan, Rong Chen, Rihong Guo, Fang Chen, Zhendan Shi, Shijia Ying","doi":"10.1016/j.psj.2024.104473","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104473","url":null,"abstract":"<p><p>This study aims to investigate the effects of different relative humidity (RH) during 4-21d of Beijing ducks on their condition at 42 d. A total of 48 Pekin duck were randomly allotted into 4 treatments (A:RH60 %, B:RH67 %,C:RH74 %,D:RH81 %), each having 3 replicates of 4 ducks. Different humidity treatments were applied from 4 to 21 days. At 42 d, there were no significant differences (P > 0.05) in body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) among the 4 groups. Oblique body length in D was significantly higher than that in A (P < 0.05). Compared with A, the chest depth of the other groups was significantly increased, and the abdominal fat weight of B and C was significantly increased (p < 0.05). Liver weight and liver index in C and D significantly higher than that in B (p < 0.05). Abdominal fat ratio in B was significantly higher than that in A (P < 0.05). Cholesterol (CHOL) in D was significantly higher than that in A and C (P < 0.05), triglyceride (TG) in C was significantly higher than that in A and B (P < 0.05), and low-density lipoprotein cholesterol (LDL-C) in B was significantly higher than that in A, C, and D (P < 0.05). pH in B was significantly higher than that in A (P < 0.05), brightness (L*) in A was significantly higher than that in B and C (P < 0.05), redness (a*) in B and C was significantly higher than that in A and D (P < 0.05), and yellowness (b*) in C was significantly lower than that in A and D (P < 0.05).The results indicate that the RH of environment during the brooding period has no impact on duck production performance but induces some oxidative stress damage and changes in meat quality. Additionally, ducks subjected to different RH treatments during the brooding period exhibit varying adaptability to the same environment as they mature.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects supplementation of novel multi-enzyme on laying performance, egg quality, and intestinal health and digestive function of laying hens. 补充新型多酶对蛋鸡产蛋性能、鸡蛋质量、肠道健康和消化功能的影响
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-31 DOI: 10.1016/j.psj.2024.104461
Qixin Huang, Wuzhou Yi, Jinghui Fan, Rui Chen, Xin Ma, Zhou Chen, Wenzi Wu, Lichun Qian
{"title":"Effects supplementation of novel multi-enzyme on laying performance, egg quality, and intestinal health and digestive function of laying hens.","authors":"Qixin Huang, Wuzhou Yi, Jinghui Fan, Rui Chen, Xin Ma, Zhou Chen, Wenzi Wu, Lichun Qian","doi":"10.1016/j.psj.2024.104461","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104461","url":null,"abstract":"<p><p>This study investigated the effects of multi-enzyme supplementation on various aspects of laying hens, including laying performance, egg quality, intestinal health and digestive function. In total, 384 Jingfen No.6 laying hens at 65-week-age were randomly assigned to four distinct dietary treatments: a basal diet (CON), CON supplemented with 150 g/t multi-enzyme (T1), CON with 300 g/t multi-enzyme (T2), and 600 g/t multi-enzyme (T3). A significant linear (P = 0.044) and quadratic (P = 0.014) increase was observed in the laying rate, while the feed/egg ratio exhibited a linear (P = 0.001) and quadratic (P < 0.001) decrease with increasing multi-enzyme supplementation. Additionally, linear (P < 0.05) and quadratic (P < 0.05) increases were observed in yolk rate and haugh unit with increasing levels of multi-enzyme supplementation. The trypsin activity in the duodenum and crude protein digestibility showed linear (P < 0.05) and quadratic (P < 0.05) increase with the addition of multi-enzyme. Furthermore, lipase and amylase activities in the duodenum increased quadratically (P = 0.041) and linearly (P = 0.040), respectively. Both jejunal and ileal digesta viscosities showed linear (P < 0.05) and quadratic (P < 0.05) decrease with the increasing addition of multi-enzyme. Moreover, multi-enzyme supplementation significantly increased (P < 0.05) the number of goblet cells in the intestinal of the treatment groups. The mRNA expression of Occludin-1, mucin 2 (MUC-2), large neutral amino acids transporter small subunit 1 (LAT-1) in the jejunum were significantly increased (P < 0.05) in the treatment groups (T1, T2 and T3) compared to the CON group. Additionally, the mRNA expression of solute carrier family 6-member 19 (B<sup>0</sup>AT-1) and large neutral amino acids transporter small subunit 4 (LAT-4) were significantly evaluated (P < 0.05) in the T2 and T3 groups, respectively. In conclusion, multi-enzyme supplementation enhanced digestive enzyme activities and intestinal barrier function, reduced intestinal digesta viscosity, and regulated mRNA expression of intestinal amino acid and lipid transporter genes, thereby improving crude protein digestibility and positively affecting laying performance and egg quality in hens.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide. 对补充丙氨酰-谷氨酰胺二肽的生长母鸡回肠适应性和生长反应的转录组分析
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-31 DOI: 10.1016/j.psj.2024.104479
Usman Nazir, Zhenming Fu, Xucheng Zheng, Muhamamd Hammad Zafar, Zhi Yang, Zhiyue Wang, Haiming Yang
{"title":"Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide.","authors":"Usman Nazir, Zhenming Fu, Xucheng Zheng, Muhamamd Hammad Zafar, Zhi Yang, Zhiyue Wang, Haiming Yang","doi":"10.1016/j.psj.2024.104479","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104479","url":null,"abstract":"<p><p>The growing phase of laying hens is crucial for growth and development due to its direct impact on their productivity during laying phase. During initial growth phase, intestinal tract undergoes rapid development which requires plenty of nutrients to help laying hens grow and mature. This study investigated the effect of Alanyl-Glutamine (Aln-Gln) levels on growth performance, ileal morphology and transcriptomic analysis of growing Hy-line brown hens. A total of 480 day old Hy-line brown chicks having similar body weight (BW) were randomly divided to be fed diets having 0%, 0.1%, 0.2% and 0.3% Aln-Gln for 6-wks (8 replicates/group, 15 birds/replicate). One bird from every pen was slaughtered and morphological parameters of ileum were evaluated. Results taken on day 42 revealed an improved average daily gain (ADG), final body weight (FBW) and feed-to-gain ratio (F/G) in the birds that consumed 0.2% and 0.3% Aln-Gln supplemented diet (P < 0.05). Ileal morphological assays showed that villus height, villus width and villus to crypts ratio (V/C) were significantly increased at 42 days of age in birds fed diets with 0.2% Aln-Gln (P<0.05). The RNA sequencing (RNA-Seq) was executed to identify differentially expressed genes (DEGs) among groups that found 2265 DEGs (1256 up-regulated; 1009 down-regulated) in ileum tissue. According to the Kyoto Encyclopedia of Genes (KEGG) and Genomic Pathway Enrichment Analysis, majority of DEGs indicated change in metabolic pathways. Genes related to growth factors, intestinal morphology and protein metabolism were up-regulated in test groups as compared to control group. In conclusion, addition of Aln-Gln to the diet improved growth performance and ileum development in growing hens; transcriptomic analysis revealed up-regulation of genes related to growth and intestinal morphology.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of the regulatory network and pathways in duodenum affecting chicken abdominal fat deposition. 十二指肠中影响鸡腹部脂肪沉积的调控网络和途径的特征。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-31 DOI: 10.1016/j.psj.2024.104463
Zhijie Liu, Sibei Cheng, Xing Zhang, Miaomiao Yang, Jixiang Wei, Fei Ye, Zheng Ma, Huimin Kang, Zhengfen Zhang, Hua Li, Hai Xiang
{"title":"Characterization of the regulatory network and pathways in duodenum affecting chicken abdominal fat deposition.","authors":"Zhijie Liu, Sibei Cheng, Xing Zhang, Miaomiao Yang, Jixiang Wei, Fei Ye, Zheng Ma, Huimin Kang, Zhengfen Zhang, Hua Li, Hai Xiang","doi":"10.1016/j.psj.2024.104463","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104463","url":null,"abstract":"<p><p>The excessive accumulation of abdominal fat in chickens has resulted in a reduction in both the feed conversion efficiency and the slaughter yield. To elucidate the regulatory mechanisms and metabolic pathways affecting abdominal fat deposition in the context of broiler breeding, a cohort of 400 Qingyuan partridge chickens with varying abdominal fat deposition was established. Whole transcriptome sequencing analyses were conducted on the duodenum of 20 representative chickens to ascertain the regulatory networks at this vital digestive and absorptive organ. Consequently, 116 differentially expressed genes were identified, exhibiting a trend of increasing or decreasing expression in correlation with the accumulation of abdominal fat. A total of 36 DEmRNAs, 170 DElncRNAs, 92 DEcircRNAs and 88 DEmiRNAs were identified as differentially expressed between chickens with extremely high and low abdominal fat deposition. The functional enrichment analyses demonstrated that the differentially expressed RNA in the duodenum were involved in the regulation of chicken abdominal fat deposition by mediating a series of metabolic pathways, including the Wnt signaling pathway, the PPAR signaling pathway, the Hippo signaling pathway, the FoxO signaling pathway, the MAPK signaling pathway and other signaling pathways that are involved in fatty acid metabolism and degradation. The construction of putative interaction pairs led to the suggestion of two lncRNA-miRNA-mRNA ceRNA networks comprising two mRNAs, two miRNAs, and 29 lncRNAs, as well as two circRNA-lncRNA-miRNA-mRNA ceRNA networks comprising 26 mRNAs, 12 miRNAs, 17 lncRNAs, and nine circRNAs, as core regulatory networks in the duodenum affecting chicken abdominal fat deposition. The aforementioned genes including TMEM150C, REXO1, PIK3C2G, ppp1cb, PARP12, SERPINE2, LRAT, CYP1A1, INSR and APOA4, were proposed as candidate genes, while the miRNAs, including miR-107-y, miR-22-y, miR-25-y, miR-2404-x and miR-16-x, as well as lncRNAs such as ENSGALT00000100291, TCONS_00063508, TCONS_00061201 and TCONS_00079402 were the candidate regulators associated with chicken abdominal fat deposition. The findings of this study provide a theoretical foundation for the molecular mechanisms of mRNAs and non-coding RNAs in duodenal tissues on abdominal fat deposition in chickens.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge. 通过水线给药的双菌株益生菌能在肉鸡面临菌群失调挑战时,有效调节回肠和盲肠微生物群、sIgA 和急性期蛋白水平以及肉鸡的生长性能。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-30 DOI: 10.1016/j.psj.2024.104462
S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs
{"title":"A dual strain probiotic administered via the waterline beneficially modulates the ileal and cecal microbiome, sIgA and acute phase protein levels, and growth performance of broilers during a dysbacteriosis challenge.","authors":"S A S van der Klein, S S Arora, S Haldar, A K Dhara, K Gibbs","doi":"10.1016/j.psj.2024.104462","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104462","url":null,"abstract":"<p><p>Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 10<sup>8</sup> colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 10<sup>8</sup> CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 10<sup>9</sup> CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of antibiotic growth promoters (AGPs) on feed conversion ratio (FCR) of broiler chickens: A meta-analysis. 抗生素生长促进剂(AGPs)对肉鸡饲料转化率(FCR)的影响:荟萃分析。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-30 DOI: 10.1016/j.psj.2024.104472
Mohamed El-Fateh, Muhammad Bilal, Xin Zhao
{"title":"Effect of antibiotic growth promoters (AGPs) on feed conversion ratio (FCR) of broiler chickens: A meta-analysis.","authors":"Mohamed El-Fateh, Muhammad Bilal, Xin Zhao","doi":"10.1016/j.psj.2024.104472","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104472","url":null,"abstract":"<p><p>Antibiotics have been used for a long time as growth promoters in the poultry industry for growth and health benefits. However, recent studies reflected little, no, or negative impacts of using antibiotics for growth promotion purposes. This study was designed to examine the overall effect of antibiotic supplementation on the feed conversion ratio (FCR) in broiler chickens as a performance indicator and assess different covariates for explaining heterogeneity in response to the use of antibiotics as growth promoters. In this regard, FCRs obtained from articles that compared diets with and without antibiotics for broiler chickens were extracted from electronic databases (PubMed, Web of Science, and Google Scholar) as per PRISMA guidelines. The database comprised 42 scientific articles containing 74 experiments totaling 19,562 chickens. A basic meta-analysis and subgroup analysis were performed to assess the overall impact of antibiotic growth promoters (AGPs) on FCR of broiler chickens while a meta-regression analysis was used to assess different covariates (breed, antibiotic dose, location, time, presence of anticoccidial in the feed, antibiotic type, and housing type) to explain variations in true effect size of the studies. The basic meta-analysis of these studies revealed an overall improvement in FCR by 2.8 % (p < 0.05) due to addition of AGPs than the control group with the confidence interval range from 1.3 to 4.3 % and the prediction interval ranging between -10 and 16 %. The covariates, including breed, dose and location, provided the optimum model fit and explained 53 % of variations in the FCR across different studies. In summary, an overall marginal improvement has been observed in mean FCR of broiler chickens along with a wide range of variations in response to use of AGPs. The variations in true effects among these studies are subject to several confounding effects, particularly breed, dose and location. Considering the marginal positive effect of antibiotic usage in chicken performance, we recommend against using of AGP.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Balancing act: Studying the effect of perch space allowance on welfare in Canadian laying strain pullets raised in floor pens with access to a single-tier perch system to 18 wk of age. 平衡行为:研究栖架空间对加拿大产蛋品系小母鸡福利的影响,这些小母鸡在18周龄前在地面鸡舍饲养,可使用单层栖架系统。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-30 DOI: 10.1016/j.psj.2024.104457
Carolin A B Adler, Tory Shynkaruk, Samantha McPhee, Kailyn Buchynski, Adelle Herr, Eugenia Herwig, Karen Schwean-Lardner
{"title":"Balancing act: Studying the effect of perch space allowance on welfare in Canadian laying strain pullets raised in floor pens with access to a single-tier perch system to 18 wk of age.","authors":"Carolin A B Adler, Tory Shynkaruk, Samantha McPhee, Kailyn Buchynski, Adelle Herr, Eugenia Herwig, Karen Schwean-Lardner","doi":"10.1016/j.psj.2024.104457","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104457","url":null,"abstract":"<p><p>The rearing environment for pullets should mirror their later production environment as closely as possible. However, existing perch space recommendations are based on data for laying hens rather than pullets. This study explores the impact of perch space allowances on the welfare of Canadian laying strain pullets raised to 18 wk. Two trials were conducted with 1,032 Lohmann Brown-Lite (LB) and LSL-Lite (LW) pullets each. A randomized complete block (trial) design was used with a 4 (perch space) × 2 (strain) factorial arrangement. Birds were raised in 16 floor pens (3 × 3 m each; 897.67 cm<sup>2</sup> per bird). Wooden perches were provided from d 1, allowing 6, 9, 12, or 15 cm perch space per pullet. Data were tested for normality, and log+1 transformed if necessary. Significance was declared at P≤0.05. Data were collected for basic health and functioning (body weight, mortality, pullet width, keel bone damage, and tibia bone parameters), affective states (comb damage, heterophil-to-lymphocyte (H/L) ratios, and behavior), and natural living (perch usage and jumping success). Perch space did not affect mortality, keel bone damage, tibia breaking strength, comb damage, or H/L ratios. At 16 and 18 wk, LB body weight slightly increased while the LW body weight decreased with increasing perch space (P=0.05 and 0.02). At 3 wk, pullets spent a higher percentage of time wing flapping on the perch when provided 15 cm compared to 6 cm (P=0.04). During the scotoperiod at 18 wk, a higher percentage of birds perched with 12 and 15 cm perch space than 6 cm (P<0.01). Pullet width at 18 wk was 12.52 cm (sitting) and 11.66 cm (standing) for the LB and 13.85 cm (sitting) and 12.94 cm (standing) for the LW. Overall, perch space allowance had minor effects on the measured welfare indicators. Based on pullet width, a minimum of 12.5 cm for LW and 13.9 cm for LB pullets should be provided to allow all pullets to sit on the perch simultaneously.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and evaluation of three multienzyme isothermal rapid amplification assays for fowl adenovirus serotype 4. 针对鸡腺病毒血清 4 型的三种多酶等温快速扩增检测方法的开发与评估。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-30 DOI: 10.1016/j.psj.2024.104452
Yin Dai, Yueyi Zhong, Fazhi Xu, Siqin Gu, Huiqin Zhou, Jieru Wang, Dongdong Yin, Lei Yin, Xuehuai Shen, Xiaocheng Pan, Xuelan Liu
{"title":"Development and evaluation of three multienzyme isothermal rapid amplification assays for fowl adenovirus serotype 4.","authors":"Yin Dai, Yueyi Zhong, Fazhi Xu, Siqin Gu, Huiqin Zhou, Jieru Wang, Dongdong Yin, Lei Yin, Xuehuai Shen, Xiaocheng Pan, Xuelan Liu","doi":"10.1016/j.psj.2024.104452","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104452","url":null,"abstract":"<p><p>Fowl adenovirus serotype 4 (FAdV-4) is the main causative agent of hydropericardium hepatitis syndrome (HHS), which has resulted in huge economic losses to the poultry industry in recent years. Hence, a rapid and simple visual detection method is needed for identification of FAdV-4. In this study, three multienzyme isothermal rapid amplification (MIRA) assays, basic MIRA, MIRA-qPCR and MIRA-LFD were developed for detection of FAdV-4. The amplification primers and reaction conditions were optimized, and the specificity and sensitivity of the assays were evaluated. The MIRA assays were specific for FAdV-4 with no cross-reaction with novel goose astrovirus, H9 subtype avian influenza virus, duck enteritis virus, Muscovy duck reovirus, or duck circovirus. The basic MIRA assay required only one primer pair and the reaction can be completed within 30 min at 36 °C. The MIRA-qPCR and MIRA-LFD assays were completed in 20 min with a minimum detection limit of 1 × 10<sup>1</sup> copies/μL and 1 × 10<sup>2</sup> copies/μL, respectively. The results of the MIRA-LFD assay can be observed directly with the naked eye, omitting the need for specialized instruments. The positive rate of three proposed MIRA assays were consistent with that of the conventional PCR assay. The MIRA assays are simple, rapid, and effective diagnostic tools for field detection of FAdV-4.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supplementation of precision biotic leads to improved growth performance by modulating the microbiome of broiler chickens fed corn or wheat-based diets 通过调节以玉米或小麦为基础日粮的肉鸡的微生物群,补充精准生物制剂提高生长性能
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-29 DOI: 10.1016/j.psj.2024.104451
C Bortoluzzi , M Segura-Wang , R Aureli , A Leduc , MA Iuspa , AJ Cowieson
{"title":"Supplementation of precision biotic leads to improved growth performance by modulating the microbiome of broiler chickens fed corn or wheat-based diets","authors":"C Bortoluzzi ,&nbsp;M Segura-Wang ,&nbsp;R Aureli ,&nbsp;A Leduc ,&nbsp;MA Iuspa ,&nbsp;AJ Cowieson","doi":"10.1016/j.psj.2024.104451","DOIUrl":"10.1016/j.psj.2024.104451","url":null,"abstract":"<div><div>The objective of the present study was to evaluate the effect of a precision biotic (PB; Symphiome®, dsm-firmenich) on the growth performance, nutrient digestibility, litter quality, blood uric acid (UA) concentration, cecal short-chain fatty acids (SCFA) concentration, and ileal and cecal microbiome of broiler chickens fed corn or wheat-based diets. One-day-old broiler chicks were placed in a completely randomized design (2 × 2 factorial scheme) in 4 treatments, 8 replicates/treatment, and 16 birds/replicate. The treatments were a corn or wheat and soybean meal-based diet without or with supplementation of 0.9 kg/MT of PB. All the birds were vaccinated against coccidiosis at the time of placement (Paracox 5). Growth performance was evaluated weekly and at the end of the experiment. At d 35, blood was collected to determine UA concentration and cecal content for SCFA concentration analysis. Ileal digesta was also collected for nutrient digestibility analysis, and ileal digesta and cecal content were collected for microbiome analysis. The data were submitted to two-way ANOVA (<em>P</em> ≤ 0.05), and LSM was used to separate the means in case of interaction. During the starter and grower phases of the study, a better body weight gain (<em>P</em> = 0.0008 and <em>P</em> = 0.04, respectively) was observed in birds fed wheat compared to corn-based diets. From 28 to 36 d of age, the supplementation of PB increased feed intake (<em>P</em> = 0.05), and tended (<em>P</em> = 0.06) to improve the feed conversion ratio by 4.3 points vs non-supplementated birds. Birds fed wheat-based diets had higher (<em>P</em> = 0.02) blood UA than corn based-diets fed birds, and the supplementation of PB led to a reduction (<em>P</em> = 0.02) of blood UA compared to non-supplemented birds. The supplementation of PB changed the abundance of core metabolic pathways of the microbiome, mostly related to protein metabolism, which led to a reduction in blood UA concentration and increase of cecal SCFA concentrations. In conclusion, by beneficially modulating the microbiome, the supplementation of PB was translated into improved growth performance of broiler chickens fed corn or wheat-based diets.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research note: Changes in chicken egg yolk metabolome during its spray drying and storage. 研究说明:鸡蛋黄代谢组在喷雾干燥和储存过程中的变化。
IF 3.8 1区 农林科学
Poultry Science Pub Date : 2024-10-29 DOI: 10.1016/j.psj.2024.104453
Beibei Wang, Xialei Liu, Shugang Li, Shijian Dong, Putri Widyanti Harlina, Jinqiu Wang, Fang Geng
{"title":"Research note: Changes in chicken egg yolk metabolome during its spray drying and storage.","authors":"Beibei Wang, Xialei Liu, Shugang Li, Shijian Dong, Putri Widyanti Harlina, Jinqiu Wang, Fang Geng","doi":"10.1016/j.psj.2024.104453","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104453","url":null,"abstract":"<p><p>The differences in metabolites between fresh egg yolk (FEY), spray-dried egg yolk powder (SEY), and stored egg yolk powder (S-SEY) were quantitatively compared through metabolomic analysis. Total of 1004 metabolites were identified in the three groups of egg yolk samples. In pairwise group analysis, 242 differential metabolites were identified in FEY and SEY, 311 differential metabolites were identified in FEY and S-SEY, and 157 differential metabolites were identified in SEY and S-SEY. The analysis of differential metabolites with the highest abundance showed that amino acids, carbohydrates and lipids in FEY would undergo oxidation reactions after spray drying and storage and thus led to significant changes in the type and abundance of metabolites. The representative differential metabolites were then screened out for judging the freshness of egg yolk powder. Therefore, the results are highly important for evaluating the quality of egg yolk powder and provide important information for understanding the nutritional changes of egg yolk after spray drying and storage.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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