Poultry Science最新文献

{"title":"Wnt signaling pathway and retinoic acid signaling pathway involved in delamination and migration of chicken trunk NCCs and contributing to HVP phenotype","authors":"Zhengyang Chen ,&nbsp;Changbin Zhao ,&nbsp;Rong Fu ,&nbsp;Chengyue Yuan ,&nbsp;Ke Zhang ,&nbsp;Xiquan Zhang","doi":"10.1016/j.psj.2025.105114","DOIUrl":"10.1016/j.psj.2025.105114","url":null,"abstract":"<div><div>Hyperpigmentation of the visceral peritoneum (<strong>HVP</strong>) is a hereditary trait that significantly affects the carcass quality in bearded chickens, yet its molecular mechanisms remain unclear. This study utilized data-independent acquisition proteomics to analyze the protein expression profiles of black peritoneum (<strong>B</strong>), faded peritoneum (<strong>F</strong>), and normal peritoneum (<strong>N</strong>) in bearded chickens at 40 and 120 d of age. Combined with histopathological and functional enrichment analyses, we revealed the regulatory network underlying HVP formation. Results indicated that the melanin content was significantly elevated in HVP samples, without accompanying inflammatory responses or tumor characteristics, suggesting that its formation is driven by developmental abnormalities. A total of 9,375 high-confidence proteins were identified through proteomics, with differentially abundant proteins at 40 d of age (219 proteins) primarily enriched in ribosomal function, tyrosine metabolism, and melanin synthesis pathways. In comparison, at 120 d of age (246 proteins), they were enriched in transcription regulation and chromatin remodeling pathways. The abnormal expression of key co-expressed proteins DHRS3 and DACT1 suggests that the dysregulation of retinoic acid (<strong>RA</strong>) and the Wnt signaling pathway may promote the directed differentiation of melanocytes by regulating neural crest cells (<strong>NCCs</strong>). The reduced abundance of the chondroitin sulfate proteoglycan, VCAN, weakened the peritoneal barrier function, whereas estradiol accelerated melanin synthesis via hormonal microenvironmental regulation. Furthermore, the formation of HVP led to a reprogramming of energy metabolism, reduced fat deposition, and a downregulation of immune-related molecules, implying that pigment deposition may weaken the chicken immune response. This study systematically elucidates the molecular mechanisms of HVP and provides potential targets for molecular breeding of HVP.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105114"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated omics reveals the regulatory role of PKCα in Sertoli cell proliferation and apoptosis through the MAPK/ERK signaling pathway in goose testis","authors":"Yupu Song ,&nbsp;Qiuyuan Liu ,&nbsp;Jingyun Ma ,&nbsp;Ichraf Mabrouk ,&nbsp;Yuxuan Zhou ,&nbsp;Xinyue Li ,&nbsp;Guizhen Xue ,&nbsp;Xiaoming Ma ,&nbsp;Jing Xu ,&nbsp;Jingbo Wang ,&nbsp;Hongxiao Pan ,&nbsp;Guoqing Hua ,&nbsp;Heng Cao ,&nbsp;Jingtao Hu ,&nbsp;Yongfeng Sun","doi":"10.1016/j.psj.2025.105123","DOIUrl":"10.1016/j.psj.2025.105123","url":null,"abstract":"<div><div>Testicular development is essential for reproductive performance in geese, as the testes are the primary organs for sperm production and play a pivotal role in egg-laying physiology. Despite their importance, genes, proteins, and pathways regulating goose testicular development are poorly understood. This study employed integrative transcriptomic and proteomic analysis methods to identify critical regulators of testicular development in geese across three reproductive periods. Additionally, the role of <em>PKCα</em> in Sertoli cell proliferation via the MAPK/ERK pathway was evaluated at the cellular level. A total of 8,921 differentially expressed genes and 1,866 differentially expressed proteins were identified, revealing key pathways such as FOXO, MAPK, PPAR, and Hedgehog that regulate testicular development. Both omics correlation analysis and signal pathway regulation network results show the importance of MAPK in this process, while cellular experiment revealed that <em>PKCα</em> affects proliferation and apoptosis of Sertoli cells through the MAPK/ERK signaling pathway. The findings revealed that <em>PKCα</em> downregulation reduced the expression of genes associated with both cell proliferation and apoptosis, resulting in a diminished activity of Sertoli cells.</div><div>This study compared testicular transcriptomes and proteomes of Hungarian and Jilin white geese, identifying key genes, proteins, and pathways critical for reproduction. These findings advance our understanding of molecular mechanisms underlying testicular development and provide insights to enhance gander reproductive performance.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105123"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay of oxytocin and dopaminergic system: Effects on food consumption in broiler chickens","authors":"Hamed Zarei ,&nbsp;Mobina Sharafinezhad ,&nbsp;Keyvan Hasani","doi":"10.1016/j.psj.2025.105139","DOIUrl":"10.1016/j.psj.2025.105139","url":null,"abstract":"<div><div>Oxytocin play a crucial physiological role in regulating feed consumption and maintaining energy homeostasis. Numerous studies have highlighted the influence of central dopaminergic systems on appetite control; however, there is a lack of information regarding their interaction with food intake regulation in avian species. Consequently, the present study aimed to investigate the potential interplay between the central oxytocin and dopaminergic systems in modulating food consumption in broiler chicks. In the initial experiment, various doses of oxytocin (2.5, 5, and 10 µg) and saline were administered via intracerebroventricular (ICV) injection to ascertain the effective dosage of oxytocin. The second experiment involved ICV injections of saline, SCH23390 (a D1 receptor antagonist), the identified effective dose of oxytocin, and a co-injection of SCH23390 with oxytocin. Subsequent experiments (3 to 7) followed a similar methodology, substituting SCH23390 with AMI-193 (a D2 receptor antagonist), NGB2904 (a D3 receptor antagonist), <span>l</span>-741,742 (a D4 receptor antagonist), 6-hydroxydopamine (a dopaminergic neurotoxin), and <span>l</span>-DOPA (a dopamine precursor), respectively. Cumulative food intake was measured over a period of 120 min post-injection. The findings revealed that ICV administration of oxytocin significantly reduced feed consumption in broilers compared to the control group (<em>P</em> &lt; 0.05). Additionally, the co-infusion of SCH23390 and 6-hydroxydopamine alongside oxytocin significantly mitigated the hypophagic effect induced by oxytocin (<em>P</em> &lt; 0.05). In contrast, AMI-193, NGB2904, <span>l</span>-741,742, and <span>l</span>-DOPA did not exhibit any significant influence on oxytocin-induced hypophagia (<em>P</em> ≥ 0.05). These findings suggest that the anorexigenic effect of oxytocin may be primarily mediated through D1 dopaminergic receptors in broilers.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105139"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residue of allocryptopine and protopine in tissues and eggs of laying hens using ultra-high performance liquid chromatography–tandem mass spectrometry","authors":"Mengting Liu ,&nbsp;Jiaxin Xu ,&nbsp;Yidan Jing ,&nbsp;Kailan Yao ,&nbsp;Zhaoshan Tang ,&nbsp;Jianguo Zeng ,&nbsp;Zihui Yang","doi":"10.1016/j.psj.2025.105124","DOIUrl":"10.1016/j.psj.2025.105124","url":null,"abstract":"<div><div>The residues of allocryptopine (ALL) and protopine (PRO) in the eggs and tissues of laying hens from the veterinary drug Bopp Total Alkali Powder® were evaluated. UPLC-MS/MS technology was used to accurately determine these components. The study found that at a dose of 400 mg/kg, PRO in eggs reached a peak of 2.8 µg/kg on the seventh day of administration and was undetectable five days after stopping the medication. ALL was not detected throughout the study. At a dose of 2000 mg/kg, one day after stopping the medication, the residues of PRO and ALL reached 16.72 µg/kg and 8.61 µg/kg, respectively. Seven days after stopping the medication, PRO was undetectable, but there was still a residue of ALL. Tissue samples showed that two hours after stopping the medication, the concentrations of both components significantly increased in all tissues but rapidly declined within 24 h. Seven days after stopping the medication, no residues were detected in all tissues. The study suggests that the risk of residue is low when using Bopp Total Alkali Powder® at a dose of 400 mg/kg. However, using a dose of 2000 mg/kg may increase the risk of residue, so it is recommended to stop the medication for at least seven days to ensure food safety.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105124"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of early life gut microbiota through inclusion of earthworms and vermicompost in broiler diets","authors":"Muhammad Zeeshan Akram ,&nbsp;Oyekunle John Oladosu ,&nbsp;Nadia Everaert ,&nbsp;Cornelia C. Metges ,&nbsp;Gürbüz Daş","doi":"10.1016/j.psj.2025.105121","DOIUrl":"10.1016/j.psj.2025.105121","url":null,"abstract":"<div><div>We previously showed that providing earthworms (<strong>EW</strong>) to broilers during early life can mitigate dietary challenges induced by soluble non-starch polysaccharides (<strong>NSP</strong>). However, whether the positive effects of providing EW are associated with changes in the gut microbial communities of broilers was not studied. This follow-up study investigated the influence of providing EW and vermicompost (<strong>VC</strong>) on gut microbiota diversity in broilers fed either a standard corn-soy based diet as positive control (<strong>CON+</strong>) or a diet rich in NSP as negative control (<strong>CON-</strong>). A total of 120 newly hatched male birds of Cobb-500 genotype were examined in two periods (<strong>P</strong>), each lasting 8 days. In P1, birds were divided into four groups: two groups received the CON+ diet (n = 30 each), the third group received CON+ plus 1 % EW (<strong>CON+EW</strong>, n = 30), and the last group received CON+ supplemented with 1 % VC (<strong>CON+VC</strong>, n = 30). Half of the birds in each group were euthanized at the end of P1, and ileal digesta were collected for microbiota analysis. In P2, one of the CON+ groups from P1 continued the same diet, while the remaining groups were switched to dietary challenge either NSP supplemented negative control CON- (n = 15), or <strong>CON-EW</strong> (n = 15) or <strong>CON-VC</strong> (n = 15). At the end of P2, the remaining birds in all groups were euthanized for ileal digesta collection. Microbial composition was assessed using 16S rRNA gene sequencing. In P1, the CON+VC exhibited a significantly higher Chao1 index compared to the CON+ and CON+EW (<em>P</em> &lt; 0.05). In P2, α-diversity metrics remained unchanged across groups (<em>P</em> &lt; 0.05), although the Chao1 index in the CON+ showed a trend toward an increase (<em>P</em> = 0.078). Analysis of β-diversity highlighted significant differences between dietary groups in P2 (<em>P</em> = 0.001). Further analysis identified differentially enriched genera, revealing that <em>Enterococcus</em> was prominent in the CON+ during P1, while <em>Lactobacillus</em> was significantly higher in the CON+EW group. In P2, the CON-EW group exhibited increased <em>Lactobacillus</em> abundance while <em>Escherichia-Shigella</em> was overrepresented in the CON- group. Functional analysis showed that the CON-EW and CON-VC diets enriched the pathways related to Nicotinamide Adenine Dinucleotide biosynthesis and fermentation to acetate and lactate, whereas the CON- increased the biosynthesis of enterobactin and aerobactin. In conclusion, dietary earthworm supplementation positively influenced gut microbiota composition and predicted functions in response to dietary challenges.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105121"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of energy density of diets and dietary Pediococcus acidilactici supplementation on productive performance, egg quality, and intestinal function in laying hens","authors":"Airong Dong ,&nbsp;Xuemei Ding ,&nbsp;Jianping Wang ,&nbsp;Qiufeng Zeng ,&nbsp;Shiping Bai ,&nbsp;Yue Xuan ,&nbsp;Shanshan Li ,&nbsp;Sharina Qi ,&nbsp;Xiaojuan Bi ,&nbsp;Chao He ,&nbsp;Keying Zhang","doi":"10.1016/j.psj.2025.105118","DOIUrl":"10.1016/j.psj.2025.105118","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of supplementing <em>Pediococcus acidilactici</em> CNCM I-4622 MA 18/5 M (<strong>PA</strong>) into diets with two energy levels on the production performance and intestinal function of Lohmann pink laying hens from 49 to 65 weeks of age. A total of 1400 hens were used in this study. A 2 × 2 + 1 experimental design with 2 metabolic energy levels (2700.00 kcal/kg, <strong>NE</strong>; 2625.00 kcal/kg, <strong>LE</strong>), two PA levels (0 mg/kg, 110 mg/kg), and one group with intermittent PA supplementation (<strong>LE-0/PA</strong>). The experiment comprised 5 treatments with 14 replications each, and each replication had 20 hens. The results showed that reducing the dietary energy level tended to decrease body weight (<em>P</em> = 0.056). However, supplementing PA in low-energy diets tended to increase body weight in 16 weeks (<em>P</em> = 0.063). The LE-0/PA group had higher laying rates and feed utilization rates compared to other groups (<em>P</em> &gt; 0.05), maybe resulting in greater economic benefits. The LE group had significantly lower eggshell strength, Haugh unit, trypsin activity, lipase activity, and tibia breaking strength compared to the NE group (<em>P</em> &lt; 0.05). Dietary supplementation with PA significantly increased eggshell strength (<em>P</em> &lt; 0.05), tibia breaking strength, villus height/crypt depth ratio (<strong>VH/CD</strong>), lipase activity, and secretory immunoglobulin A (<strong>SIgA</strong>) content in the jejunum (<em>P</em> &lt; 0.05). Additionally, PA supplementation significantly reduced the expression of interleukin 8 (<strong><em>IL-8</em></strong>), tumor necrosis factor-α (<strong><em>TNF-α</em></strong>), toll-like receptor 4 (<strong><em>TLR-4</em></strong>), and nuclear factor kappa-B (<strong><em>NF-κB</em></strong>) mRNA (<em>P</em> &lt; 0.05). In conclusion, reducing the dietary energy level of hens can reduce the body weight and feed utilization efficiency. However, dietary PA supplementation improved body weight, reduced the expression of intestinal inflammation-related factors, and enhanced intestinal health, particularly in low-energy diets. Intermittent PA supplementation may enhance performance benefits, suggesting its potential as a strategy to optimize production performance and intestinal health in hens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105118"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Butyric acid from ligilactobacillus animalis 2020MB acts on membrane BamA to control avian pathogenic escherichia coli","authors":"Haichang Yin ,&nbsp;Zunhe Du ,&nbsp;Xinjie Jiang ,&nbsp;Yao Zhou ,&nbsp;Zhenhua Jin ,&nbsp;Feng Cong","doi":"10.1016/j.psj.2025.105119","DOIUrl":"10.1016/j.psj.2025.105119","url":null,"abstract":"<div><div>Avian pathogenic <em>Escherichia coli</em> can cause high morbidity, mortality, and serious economic losses to the global poultry industry. Lactic acid bacteria inhibit the growth of many pathogens, including <em>E. coli</em>, but the underlying mechanism remains unclear. In this study, we investigated the effect of the cell-free supernatant of <em>Ligilactobacillus animalis</em> 2020MB isolated from the intestinal tract of chickens on specific pathogen-free chickens infected with <em>E. coli</em>. The cell-free supernatant-induced inhibition of <em>E. coli</em> infection was determined through clinical symptom observation, pathological analysis, and qPCR. Protease and heat treatments did not affect the antibacterial activity of cell-free supernatant, suggesting that an organic acid was the antibacterial substance. Liquid chromatography–mass spectrometry and non-targeted metabolomics identified antibacterial activity for eight <em>L. animalis</em> 2020MB cell-free supernatant metabolites, including butyric, valeric, and succinic acids. The inhibitory activity of butyric acid was quantified by determining the minimal inhibitory concentration. Scanning electron microscopy, laser confocal microscopy, and proteomic analysis revealed that butyric acid altered the morphology and impaired the cell envelope integrity of target bacteria, leading to leakage of intracellular contents. BamA was identified as the membrane protein target for butyric acid. The findings reveal the molecular mechanism of action of <em>L. animalis</em> 2020MB in the chicken intestine against <em>E. coli</em>.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105119"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Pyrococcus furiosus Argonaute coupled with RPA for highly sensitive and specific detection of Mycoplasma gallisepticum","authors":"Yanli Zhao ,&nbsp;Dehai Su ,&nbsp;Min Li ,&nbsp;Qin Deng ,&nbsp;Shiyu Xiong ,&nbsp;Youshu Feng ,&nbsp;Hongxia Jiang ,&nbsp;Huanzhong Ding","doi":"10.1016/j.psj.2025.105116","DOIUrl":"10.1016/j.psj.2025.105116","url":null,"abstract":"<div><div><em>Mycoplasma gallisepticum</em> (<strong>MG</strong>) is the pathogen that causes chronic respiratory disease (<strong>CRD</strong>) in chickens and infectious sinusitis in turkeys. Clinical symptoms such as cough and dyspnea cause a decrease in the egg production rate and carcass quality of chickens, which results in considerable economic losses to the poultry industry. Thus, it is urgent to establish a fast, accurate and sensitive method for detecting MG. In this study, the detection of MG by combining recombinase polymerase amplification (<strong>RPA</strong>) with argonaute from <em>Pyrococcus furiosus</em> (<strong><em>Pf</em>Ago</strong>) was established that allows visual observation under blue and UV light. The sensitivity of RPA-<em>Pf</em>Ago reached 1 copy/<em>µ</em>L. After the chicken infection model, it was found that tracheal inhalation was superior to eye and nose drips and sprays, the results of RPA-<em>Pf</em>Ago and qPCR were completely consistent. This study provides a reference tool for the monitoring of MG in the clinic and contributes to the early diagnosis of the disease.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105116"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The landscape of RNA 5-methylcytosine modification during chicken embryonic myogenesis","authors":"Hongxia Jiang ,&nbsp;Jing Liu ,&nbsp;Yuxiang Wang ,&nbsp;Zurong Liao ,&nbsp;Xiaoyun Xiao ,&nbsp;Zhenxvan Ding ,&nbsp;Xvwen Huang ,&nbsp;Wenwei Li ,&nbsp;Zikun Wang ,&nbsp;Jingzhan Liang ,&nbsp;Jiguo Xu ,&nbsp;Xiaolong Hu ,&nbsp;Huirong Mao ,&nbsp;Sanfeng Liu ,&nbsp;Biao Chen","doi":"10.1016/j.psj.2025.105109","DOIUrl":"10.1016/j.psj.2025.105109","url":null,"abstract":"<div><div>Skeletal muscle is a vital protein source for human diets, making its development a significant focus in poultry research. This study examines the effects of RNA 5-methylcytosine (m5C) modifications on chicken skeletal muscle development at two critical embryonic stages: before myoblast differentiation (E10) and after differentiation (E19). By employing MeRIP-seq, we analyzed the dynamic distribution of m5C modifications within the leg muscle transcriptome, uncovering notable differences in modification states between these two stages. Our results indicate that m5C modifications are widespread in chicken skeletal muscle transcriptome and present a unique distribution pattern. Unlike N6-methyladenosine modifications, which typically show a negative correlation with RNA expression, m5C modifications exhibited a weak positive correlation in our findings. Additionally, we identified multiple m5C peaks on important epigenetic regulators, including <em>DNMT3A, DNMT3B, DNMT1, TET3</em>, and <em>METTL3</em>. This observation suggests that RNA m5C may interact with these genes to jointly influence chicken skeletal muscle development. Furthermore, we identified key genes linked to m5C peaks that are enriched in pathways associated with cell cycle regulation, muscle growth, and lipid metabolism. This study provides valuable insights into the role of m5C modifications in the development of chicken skeletal muscle and highlights their potential for coordinating myogenesis and lipid metabolic processes.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105109"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drying methods affected the fatty acids oxidation and bacterial community of traditional dry-cured ducks during processing and its relation with the aroma formation","authors":"Linyue Qi ,&nbsp;Liangwei Xu ,&nbsp;Hongbing Chen ,&nbsp;Haixing Li ,&nbsp;Yikui Lai ,&nbsp;Xiaohua Liu","doi":"10.1016/j.psj.2025.105110","DOIUrl":"10.1016/j.psj.2025.105110","url":null,"abstract":"<div><div>The formation of volatile compounds in dry-cured ducks produced by hot and cold air circular drying (HCAD), hot air drying (HAD), and traditional sun drying (TSD) were analyzed by E-nose and GC-MS. The HCAD exhibited higher alcohols, aldehydes, esters, and furans than that of the other two. Six, five, and three key compounds were identified by OAV in HCAD, HAD, and TSD, respectively. Nonanal was the highest relative content of aldehydes in all groups. (<em>E</em>)-2-octenal was detected only in HCAD. HAD accelerated the oxidation of fatty acids. The POV and AV of HAD were 0.24 g/100 g and 2.02 mg/g when stored for 15 days, which were higher than that of HCAD and TSD. <em>Proteobacteria, Actinobacteriota</em> and <em>Firmicutes</em> were the most abundant phylum, <em>Psychrobacter, Kocuria</em>, and <em>Macrococcus</em> were the primary dominant genus. The hightest abundance of <em>Firmicutes</em> and <em>Macrococcus</em> in the HCAD-15d might contribute to the aroma formation. HCAD showed a better quality and flavor than TSD and HAD, indicating it is an innovative method for producing dry-cured duck.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 6","pages":"Article 105110"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}