Poultry Science最新文献

{"title":"Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection.","authors":"Thi Hao Vu, Chaeeun Kim, Anh Duc Truong, Jun-Mo Kim, Hyun S Lillehoj, Yeong Ho Hong","doi":"10.1016/j.psj.2024.104524","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104524","url":null,"abstract":"<p><p>Avian influenza viruses (AIVs) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of immune responses, yet their roles in AIV-infected chickens remain poorly understood. This study aimed to investigate the expression profiles of lncRNAs and their targets in Vietnamese Ri chickens infected with the highly pathogenic AIV (HPAIV) H5N1. Through RNA sequencing, we identified novel lncRNAs and analyzed differentially expressed (DE) transcripts at 1 and 3 days post-infection (dpi) in chicken lung tissue. Our results revealed a higher number of DE lncRNAs and mRNAs at 1 dpi and 3 dpi, respectively, compared to control, with resistant chickens exhibiting a notably stronger immune response than susceptible chickens at 3 dpi. Functional analysis implicated these lncRNAs in immune-related pathways crucial for host responses to H5N1 viral infection. Furthermore, we identified lncRNA-mRNA interactions associated with antiviral responses and immune function. Notably, several genes involved in antiviral resistance and immune responses showed higher expression in resistant chickens, confirming their stronger antiviral response. Overall, our study provides insights into the role of lncRNAs in the host's response to HPAIV H5N1 infection in chickens and highlights potential candidates for further investigation into host-pathogen interactions. These findings could drive the development of novel control strategies for AIVs, significantly enhancing poultry health and biosecurity.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"104524"},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low protein diet influences mineral absorption and utilization in medium-growing yellow-feathered broilers from 1 to 30 days of age.","authors":"Xiaoran Li, Chong Wang, Shunying Li, Liyang Zhang, Xiudong Liao, Lin Lu","doi":"10.1016/j.psj.2024.104512","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104512","url":null,"abstract":"<p><p>Reduced-protein diet can save protein ingredients and reduce nitrogen (N) losses. However, the effect of low protein diet on the mineral uptake and utilization in broilers needs to be explored. The aim of this study was to investigate the effect of low-protein diet on the growth performance, N deposition, mineral contents in serum, tissues and excreta, and the activities and gene expression of related enzymes in tissues of medium-growing yellow-feathered broilers, so as to elucidate the relationship between dietary protein level and the absorption and utilization of minerals in broilers. A total of 72 1-d-old Spotted-Brown male broilers were randomly allotted to 1 of 2 treatments with 6 replicate cages of 6 birds per cage for each treatment. The dietary crude protein (CP) levels for the two treatments were 21 % (the control treatment) and 19 % (low protein treatment), respectively. The experimental period was 30 d. The results showed that no differences (P > 0.05) were detected in average daily gain, average daily feed intake and feed: gain ratio of broilers during 1 to 30 d between the two treatments. However, low protein intake increased (P < 0.05) N retention rate, serum P, Cu and Mn, and excreta Cu, Mn and Zn, and decreased (P < 0.05) liver P and excreta P. In addition, birds fed low protein diet had higher (P < 0.05) manganese superoxide dismutase, and total superoxide dismutase activities in liver, and total antioxidant capacity and malondialdehyde content in heart, and lower (P < 0.05) copper-zinc superoxide dismutase (CuZnSOD) and succinate dehydrogenase activities in liver and CuZnSOD mRNA level in heart. In conclusion, the reduction of dietary CP content from 21 % to 19 % improved N retention, the absorption of P, Cu and Mn, as well as the antioxidant ability of liver and heart, and influenced metabolic utilization of P, Cu, Zn, Fe and Mn in medium-growing yellow-feathered broilers from 1 to 30 d of age.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104512"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Simultaneous detection of GPV, H5 AIV, and GoAstV via TaqMan probe-based multiplex qPCR","authors":"Xiaoquan Wang ,&nbsp;Miao Cai ,&nbsp;Xiaolong Lu ,&nbsp;Qianqian Xu ,&nbsp;Yanhong Wang ,&nbsp;Wenhao Yang ,&nbsp;Kaituo Liu ,&nbsp;Ruyi Gao ,&nbsp;Yu Chen ,&nbsp;Jiao Hu ,&nbsp;Min Gu ,&nbsp;Shunlin Hu ,&nbsp;Xiufan Liu ,&nbsp;Xiaowen Liu","doi":"10.1016/j.psj.2024.104511","DOIUrl":"10.1016/j.psj.2024.104511","url":null,"abstract":"<div><div>The endemic status of goose parvovirus (GPV), H5 subtype avian influenza virus (AIV), and goose astrovirus (GoAstV) infections continues to devastate the poultry industry in China. Despite this, there exists a notable gap in the application of molecular diagnostic techniques. This investigation described the development of a multiplex qualitative polymerase chain reaction (qPCR) assay capable of concurrently detecting GPV, H5 AIV, and GoAstV, with no cross-reactivity observed with other avian viral pathogens. The assay exhibited a detection threshold of 10 copies/μL for both GPV and GoAstV, and 1 copy/μL for H5 AIV. The intra- and inter-assay coefficients of variation were &lt; 3.0%, signifying high repeatability within and across assay batches. Utilizing this multiplex qPCR assay, a batch of 60 clinical samples was analyzed to assess its practical utility. The detected prevalence rates for GoAstV, GPV, and H5 AIV were 35.0% (21/60), 21.7% (13/60), and 15.0% (9/60), respectively. Concurrent infections were also identified, with rates for GPV + GoAstV, GPV + H5 AIV, GoAstV + H5 AIV, and GPV + GoAstV + H5 AIV being 6.7% (4/60), 3.3% (2/60), 3.3% (2/60), and 3.3% (2/60), respectively. The developed multiplex qPCR assay exhibited a diagnostic concordance rate equivalent to that of traditional PCR techniques. This novel assay serves as a rapid, efficient, specific, and sensitive tool for the detection of prevalent goose viruses, thereby enhancing disease management strategies and epidemiological monitoring efforts.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104511"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A PLIN1 polymorphism is associated with fat production in male emus","authors":"Yuichi Koshiishi ,&nbsp;Ryo Takahashi ,&nbsp;Michiko Murata-Okubo ,&nbsp;Yuichi Kameyama ,&nbsp;Kousaku Souma ,&nbsp;Hiroki Hirayama ,&nbsp;Kenta Wada","doi":"10.1016/j.psj.2024.104513","DOIUrl":"10.1016/j.psj.2024.104513","url":null,"abstract":"<div><div>The emu (<em>Dromaius novaehollandiae</em>) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (<strong><em>PLIN1</em></strong>) polymorphisms in the emus. We determined the partial complementary DNA (<strong>cDNA</strong>) sequence of the <em>PLIN1</em>, which is involved in lipid droplet formation. We identified four nucleotide substitution sites (c.270C&gt;T, c.321T&gt;C, c.587A&gt;T, and c.639C&gt;T) in the <em>PLIN1</em> gene of emus. Of these, c.587A&gt;T is a non-synonymous substitution that converts lysine to methionine at the 196th codon (p.K196M). Although p.K196M was predicted to affect the production traits of emus, a large deflection in genotype frequency was observed in this study; thus, we could not investigate the relationship between genotypes and phenotypes. In males, the fat yields of the CC, CT, and TT genotypes in c.270C&gt;T were 0.25 ± 0.06, 0.22 ± 0.06, and 0.21 ± 0.07 kg, respectively, while the meat yields of the CC, CT, and TT genotypes in c.270C&gt;T were 0.15 ± 0.01, 0.16 ± 0.02, and 0.16 ± 0.03 kg, respectively. These results indicate that male emus with the CC genotype had a significantly higher fat content and lower meat productivity than male emus with the other genotypes (<em>P</em> &lt; 0.05). Therefore, c.270C&gt;T in <em>PLIN1</em> affects fat and meat production in males. Our findings may contribute to the effective genetic improvement of the emus.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104513"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin nuclear receptors mediate monochromatic light-induced T-lymphocyte proliferation of thymus through the AKT/GSK3β/β-catenin pathway in chick","authors":"Juanjuan Xiong ,&nbsp;Zixu Wang ,&nbsp;Yulan Dong ,&nbsp;Jing Cao ,&nbsp;Yaoxing Chen","doi":"10.1016/j.psj.2024.104507","DOIUrl":"10.1016/j.psj.2024.104507","url":null,"abstract":"<div><div>Based on previous research, it's unclear about the signaling pathway involved in the negative regulation of T-lymphocyte proliferation in thymus by monochromatic red light. Newly hatched chicks were randomly assigned divided into white (<strong>WL</strong>), red (<strong>RL</strong>), green (<strong>GL</strong>), and blue (<strong>BL</strong>) light treatments. Three days later, each light treatment group was further divided into intact, sham operation, and pinealectomy groups. The findings revealed that RL led to an increase in the expression of RORα and RORγ, while p-AKT/p-GSK3β/β-catenin/CyclinD1 expression in the thymus of chicks were decreased. Conversely, GL showed opposite results compared to RL. After pinealectomy, accompanied with the expression of RORα and RORγ increased under four light, p-AKT/ p-GSK3β/ β-catenin/ CyclinD1 expression were decreased. In vitro, exogenous melatonin increased the p-AKT/β-catenin/CyclinD1 expression in the thymic lymphocytes of chick reared under RL. The stimulative effect of melatonin was enhanced by SR3335 (RORα antagonist) or GSK298 (RORγ antagonist), while it was attenuated by SR1078 (RORα/RORγ agonist), LY-294 (PI3K antagonist) and HY-102 (AKT antagonist). These results demonstrate that RORα/RORγ negatively regulate monochromatic red light induced-T-lymphocyte proliferation in the thymus, possibly through the PI3K/AKT/p-GSK3β (Ser9) signaling pathway.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104507"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Note: Proteases effect on soybean meal: enzyme product and area of production differences on standardized ileal amino acids digestibility of broiler chickens.","authors":"Hong Liu, Murtala Umar Faruk, Levy Teixeira, André Fávero, Sergio L Vieira","doi":"10.1016/j.psj.2024.104493","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104493","url":null,"abstract":"<p><p>This study evaluated the effects of two protease products added to feeds having two soybean meal (SBM) sources (South and North of Brazil). A total of five hundred and sixty, 21 d male broiler chickens were allocated into 7 dietary treatments: a nitrogen-free diet (NFD) and other six diets prepared with a 40% replacement of the NFD with each SBM source. Two commercial protease products (RONOZYME® ProAct and ProAct 360^TM) were added at 15,000 PROT and 30,000 NFP per kg feed, respectively. After 5-day diet adaptation, all birds were euthanized for ileal digesta collection. Results show that the North SBM sample was inherently higher (P < 0.05) in apparent ileal digestibility (AID) of dry matter (DM), nitrogen (N), and the standardized ileal amino acid digestibility (SIAAD) compared to South SBM sample. The DM digestibility and SIAAD of North SBM sample were not different from the un-treated control and protease treatments (P > 0.05, except Val and Ile, which had higher digestibility with ProAct 360). The DM digestibility and SIAAD from South SBM sample were increased (P < 0.05, except Met, Asp and Cys) with both supplemental proteases. Correlation analysis showed that AA digestibility of the two SBM samples were negatively associated with the effect of protease products. These data demonstrated that the SIAAD of SBM was influenced by SBM source with the South SBM sample being lower than that from the North while the protease effects were more evident in the SBM sample from South; responses of ProAct 360 were superior to ProAct. Exogenous proteases can enhance nutritional value of SBM, particularly for those with inherently low amino acid digestibility.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104493"},"PeriodicalIF":3.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of black soldier fly (Hermetia illucens) larvae meal and oil on the performance, biochemical profile, intestinal health and gut microbial dynamics in laying hens.","authors":"Samiullah Khan, Xiaoli Shi, Renlian Cai, Zhao Shuai, Wei Mao, Ibrar Muhammad Khan, Ayman A Swelum, Jianjun Guo","doi":"10.1016/j.psj.2024.104460","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104460","url":null,"abstract":"<p><p>This study investigated the effect of incorporating black soldier fly (BSF) larvae meal and oil on laying hens' performance, egg quality, serum profile, intestinal structure, and gut health. A total of 378 Lohmann laying hens (age 48 wk) were randomly assigned to 6 treatments with 3 replicates of 21 hens each. Following 7 d acclimation, the trail was conducted for 8 weeks. The dietary groups include: basal corn-soybean meal diet (S) without BSF (BSO) oil (S+BSO 0), S with BSF oil (S+BSO 100), BSF meal (9 %) without BSF oil (BSF 9+BSO 0), BSF meal (9 %) with BSF oil (BSF 9+BSO 100), BSF meal (18 %) without BSF oil (BSF 18+BSO 0), and BSF meal (18 %) with BSF oil (BSF 18+BSO 100). The results showed that the BSF 18 + BSO 100 diet significantly reduced egg weight (P < 0.001) compared to other dietary treatments. The addition of BSF meal reduced feed intake (P < 0.001) and the Haugh units (P < 0.05) in hens fed 18 % BSF meal with and without BSO. The jejunum villus area, crypt depth, and intestinal wall thickness increased with the increase in the inclusion of BSF larvae meal (P < 0.001). The ileum villus height, crypt depth and intestinal wall thickness increased (P < 0.001) at 9 % BSF meal and then decreased at 18 % BSF meal with and without BSF oil. The bacteria genera Ruminococcus, Clostridiales, Bacteroidales, Ruminococcus torques, and Intestinimonas were positively associated with the dietary treatments, while Prevotellaceae UCG-001, Clostridium, and Faecalibacterium were negatively associated with the dietary treatments. The inclusion of BSF meal and oil enriched the functional network of several pathways, including ascorbate and aldarate metabolism, D-arginine and D-ornithine metabolism, and fatty acid metabolism, highlighting the positive effects of BSF larvae meal and oil on the chicken gut microbiota. In conclusion, BSF meal at 9 % with BSF oil and BSF meal at 18 % without BSF oil can be incorporated into the diet without impairing the performance and gut health of laying hens.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104460"},"PeriodicalIF":3.8,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The proteome and metabolome changes distinguish the effect of dietary energy levels on the development of ovary in chicken during sexual maturity.","authors":"Lingqian Yin, Xinyu Wang, Donghao Zhang, Zhongzhen Lin, Yan Wang, Chunlin Yu, Hang Jie, Feng Xu, Chaowu Yang, Yiping Liu","doi":"10.1016/j.psj.2024.104495","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104495","url":null,"abstract":"<p><p>To deeply understanding the impact of peripheral energy level on the development of ovaries during the sexual maturation of chicken, in this study, the ovaries and serum of sexually mature and immature chickens at the same age from different energy level groups were collected, and the proteome and metabolome were detected. The results of ovarian and serum metabolomics revealed that dietary energy levels affected the energy metabolism and fatty acid oxidation of ovary in chicken, including the up-regulated expression of dihydroacetone phosphate and α-linolenic acid in high energy level groups. The results of proteomics showed that peripheral energy levels affected the catecholamine biosynthesis and metabolism in ovary before sexual maturation. The integrating analysis revealed that increased energy flux may influence ovarian development by regulating cholesterol reserves and steroid hormone synthesis in the ovaries. In vitro, the cultivation of chicken primary granulosa cells showed that sterol carrier protein 2 played a role in fatty acid synthesis and metabolism but did not significantly affect progesterone synthesis. Overall, dietary energy levels may be involved in the development of the ovaries during sexual maturation by influencing energy metabolism, biosynthesis of unsaturated fatty acids and steroid hormone within the ovaries.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104495"},"PeriodicalIF":3.8,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1","authors":"Longxiao Hu ,&nbsp;Dandan Li ,&nbsp;Qingqing Wei ,&nbsp;Li Kang ,&nbsp;Yi Sun ,&nbsp;Yunliang Jiang","doi":"10.1016/j.psj.2024.104501","DOIUrl":"10.1016/j.psj.2024.104501","url":null,"abstract":"<div><div>Insulin-like growth factor binding protein-2 (<strong>IGFBP-2</strong>), a binding protein of insulin-like growth factor (<strong>IGF</strong>) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel <em>IGFBP-2</em> transcript and the effect of reproductive hormones and insulin-like growth factor 1 (<strong>IGF1</strong>) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of <em>IGFBP-2</em> was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (<strong>Post-GCs</strong>) than in pre-hierarchical granulosa cells (<strong>Pre-GCs</strong>). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (<strong>P4</strong>) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of <em>IGFBP-2</em> was inhibited by estradiol (<strong>E2</strong>) and follicle-stimulating hormone (<strong>FSH</strong>), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of <em>IGFBP-2</em> synergistically. Knockdown of <em>IGFBP-2</em> attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (<strong><em>CYP11A1</em></strong>). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104501"},"PeriodicalIF":3.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles.","authors":"Xinyue Hu, Yali Liu, Bincheng Tang, Jiwei Hu, Hua He, Hehe Liu, Liang Li, Shenqiang Hu, Jiwen Wang","doi":"10.1016/j.psj.2024.104498","DOIUrl":"https://doi.org/10.1016/j.psj.2024.104498","url":null,"abstract":"<p><p>Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated. Therefore, the purpose of the present study was to conduct transcriptome sequencing in pectoral muscles of both Landes (LD) and Sichuan White (SW) geese at 6, 10, and 30 weeks of age to reveal the molecular mechanisms regulating pectoral muscle hypertrophy through intra-breed and inter-breed bioinformatics analyses. Phenotypically, the pectoral muscle weight/index of LD and SW geese increased from 6 to 30 weeks of age, and except for the pectoral muscle index at 10 weeks of age (P = 0.962), at the same age, the pectoral muscle weight/index of LD geese were significantly higher than that of SW geese (P < 0.05). In transcriptional regulation, intra-breed bioinformatics analysis identified 3331 genes whose expression levels were opposite to the trend of pectoral muscle hypertrophy both in LD and SW geese, and the 3331 genes were mainly enriched into abundant KEGG pathways related to lipid metabolism, proliferation/apoptosis, and immune response. Moreover, 23 genes (including SLC2A10, TNFRSF1A, PRKAA1, SLC27A4, ITGB2, THY1, RHOA, MYL10, ACTB, PRKCB, PIK3R2, RAC2, DMD, LATS2, YAP1, WWTR1, SMAD7, CTGF, FGF1, AXIN2, GLI2, ID2, and CCND2) who were enriched in 6 crosstalk pathways named viral myocarditis, insulin resistance, sphingolipid signaling pathway, hippo signaling pathway, chemokine signaling pathway, and leukocyte transendothelial migration were identified as the key candidate genes regulating the hypertrophy of goose pectoral muscles. In inter-breed bioinformatics analysis, abundant different expression genes (DEGs) related to lipid metabolism, immune response, and proliferation/apoptosis were identified between LD and SW geese too, and compared with SW geese, the expression level of MYL10 in LD geese was lower, while the expression levels of GLI2/CTGF/SMAD7 in LD geese were higher. These results suggested that the hypertrophy of goose pectoral muscles might be achieved through more lipid deposition and less leukocyte infiltration to promote the proliferation of cells within the muscles, and the low expression of MYL10 and high expressions of GLI2/CTGF/SMAD7 might the keys to induce the pectoral muscle hypertrophy of LD geese from 6 to 30 weeks of age over that of SW geese. All data the present study obtained will provide new insights into the molecular mechanisms regulating the hypertrophy of goose pectoral muscles.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104498"},"PeriodicalIF":3.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}