Poultry Science最新文献

{"title":"HVT-delivered chicken IGF-1 improves growth performance in broilers.","authors":"Panpan Yang, Mengjia Xiang, Minghe Xu, Yanfang Cong, Xingyu Li, Yan Li, Junjie Liu, Yutao Zhu, Luyao Qiu, Qilong Qiao, Jianli Li, Baiyu Wang, Jun Zhao","doi":"10.1016/j.psj.2025.105630","DOIUrl":"10.1016/j.psj.2025.105630","url":null,"abstract":"<p><p>The domesticated poultry is invaluable as a global protein source. Growth performance in poultry, particularly broilers, plays a critical role in production efficiency. Insulin-like growth factor-1 (IGF-1) is a multifunctional hormone which shows promotive effects on growth and development. Traditional IGF-1 delivery methods, such as injections or dietary supplements, face practical limitations including high cost, multiple dosages and rapid degradation. To explore a more efficient IGF-1 delivery method for promoting chicken growth, we generated a recombinant herpesvirus of turkey expressing chicken IGF-1, rHVT-chIGF-1. A single dose of rHVT-chIGF-1 was administered intramuscularly to broilers at day 1 of age. Growth performance parameters, including body weight (BW) and average daily weight gain (ADG) was assessed over a 42-day period. The results showed that, at both 35 and 42 days of age, the rHVT-IGF-1 group showed significantly higher BW and ADG compared to the HVT group (35d BW: p = 0.0063; 42d BW: p < 0.0001; 35d ADG: p < 0.0001; 42d ADG: p < 0.0001) and the blank control group (35d BW: p = 0.0189; 42d BW: p = 0.0032; 35d ADG: p < 0.0001; 42d ADG: p < 0.0001). The cross-sectional area (CSA) of pectoral and leg muscle fibres was significantly greater in chickens from the rHVT-chIGF-1 group compared to those in the HVT vector (p = 0.0078) and blank control (p = 0.025) groups. Additionally, the mRNA expression of muscle development-related genes was significantly upregulated in the rHVT-chIGF-1 group. These findings suggest that rHVT-chIGF-1 is a novel and effective in vivo delivery system for promoting chicken growth, offering an alternative to traditional IGF-1 delivery methods.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105630"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter regarding \"Taqman-probe-based multiplex real-time RT-QPCR for simultaneous detection of duck tembusu virus, novel duck reovirus, duck hepatitis A type 1 virus and duck hepatitis A type 3 virus\".","authors":"Dr Parth Aphale, Himanshu Shekhar, Shashank Dokania","doi":"10.1016/j.psj.2025.105662","DOIUrl":"10.1016/j.psj.2025.105662","url":null,"abstract":"","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105662"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capacity enhancement on biosecurity for line supervisors working in commercial broiler farming Gujarat.","authors":"Binal Prajapati, Akash Golaviya, Khushbu Rana, Prakash Koringa, Fiona Tomley, Ayona Silva-Fletcher, Haidaruliman Paleja","doi":"10.1016/j.psj.2025.105596","DOIUrl":"10.1016/j.psj.2025.105596","url":null,"abstract":"<p><p>Poultry farming in Gujarat faces persistent challenges from infectious diseases, foodborne pathogens, and the growing threat of antimicrobial resistance (AMR), necessitating the adoption of stringent biosecurity measures encompassing isolation, traffic control, and sanitation. The study aimed to evaluate the knowledge and effectiveness of biosecurity practices among line supervisors involved in contractual commercial broiler farming in Gujarat. This study evaluated the socio-economic characteristics and biosecurity knowledge of 33 line supervisors through a one-day capacity-building training program. Pre- and post-training assessments, including structured questionnaires and Likert scales, were analyzed using descriptive statistics, and Factor Analysis of Mixed Data (FAMD) in R (v4.0.2). Results revealed that most participants were young (mean age 33.94), married males with higher secondary or graduate education and over a decade of poultry farming experience. Post-training evaluations showed a significant improvement in biosecurity knowledge and practices, with 75.76 % rating the training as excellent and was recognized as useful by 90.91 % of respondents. Furthermore, for the non-implementation of comprehensive biosecurity measures by the farmers, lack of awareness was the major attribute given by line supervisors (57.58 %) for the farmers, followed by inadequate control (51.52 %) and insufficient knowledge (48.48 %). Additionally, FAMD identified 76 epidemiological points across breeding farms, commercial broiler farms, and markets, offering insights into zoonotic risk and poultry distribution network (PDN) structure. The findings investigated the need for structured training and advisory support, along with government-led financial incentives, to strengthen biosecurity implementation in poultry systems.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105596"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Epidemiology and genotypic diversity of duck tembusu virus in geese in Partial Areas of Guangdong Province, Southern China.","authors":"Zhi-Hang Lv, Chen-Chen Yang, Xue-Lian Zhang, Jian-Wei Shao","doi":"10.1016/j.psj.2025.105625","DOIUrl":"10.1016/j.psj.2025.105625","url":null,"abstract":"<p><p>Since emerging in China in 2010, Duck Tembusu Virus (DTMUV) has caused substantial economic losses to the poultry industry. Guangdong province, a major hub for waterfowl farming and consumption in China, has been severely impacted by DTMUV. Investigating the epidemiology and genetic diversity of DTMUV in Guangdong is essential for developing effective prevention and control strategies. In this study, we collected 427 goose liver samples from five geographically distinct regions in Guangdong province. DTMUV RNA was detected in 69 samples, with positive rates ranging from 12.9 % to 18.6 % in the regions. Eight complete genome sequences were subsequently amplified, and genetic analyses revealed that these strains clustered into three distinct subclades (2.1.1, 2.2, and 3.2), indicating significant genetic heterogeneity among DTMUV circulating in Guangdong's goose populations. Furthermore, a statistically supported recombination event was identified in one strain, underscoring the role of genetic recombination in driving viral diversity. These findings enhance our understanding of DTMUV's genetic diversity and evolutionary dynamics in goose populations, thereby providing critical insights into its transmission patterns and evolutionary trends in Guangdong province, China.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105625"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision optimization of standardized ileal digestible lysine:methionine: Threonine ratios in low-protein male broiler diets using central composite design.","authors":"Chenghe Chang, Yifan Feng, Shen Wang, Anrong Zhang, Zhenlong Wu, Ying Yang","doi":"10.1016/j.psj.2025.105607","DOIUrl":"10.1016/j.psj.2025.105607","url":null,"abstract":"<p><p>This study aimed to determine the optimal standardized ileal digestible (SID) ratios of lysine (dLys), methionine (dMet), and threonine (dThr) in low-protein diets (17 % crude protein, CP) to optimize growth performance, feed efficiency, and economic viability in Arbor Acres Plus male broilers aged 22-42 days. Using a 3-factor, 5-level central composite design (CCD) within a response surface methodology (RSM) framework, 960 male broilers were randomly divided into 16 treatments, each comprising 6 replicates with 10 birds per replicate. The treatments included a control group (Con) fed a 19 % CP diet, and 15 dietary treatments with graded levels of dLys (0.97-1.23 %), dMet (0.43-0.53 %), and dThr (0.64-0.84 %). Results indicated that dLys had the strongest linear effect on body weight gain (BWG; P < 0.001). The optimal SID ratios were determined as dMet/dLys = 0.41 (0.468 %/1.145 %) and dThr/dLys = 0.64 (0.730 %/1.145 %) for BWG, and dMet/dLys = 0.38 (0.454 %/1.180 %) and dThr/dLys = 0.64 (0.757 %/1.180 %) for FCR. These optimizations enabled a 10.5 % reduction in dietary CP level without compromising growth performance, along with a 32.3 % decrease in nitrogen (N) excretion compared with the Con group. Furthermore, apparent total tract digestibility (ATTD) of amino acids (AA) improved significantly compared with the Con group (P < 0.001). In summary, the findings suggest that precise AA balancing, with a priority on lysine (Lys) sufficiency, could sustain broiler productivity in low-protein systems while reducing feed costs and environmental footprint.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105607"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Chicken seminal plasma metabolome and fertility-enhancing effects of l-carnitine supplementation in semen.","authors":"Yunlei Li, Lijun Jiang, Yunhe Zong, Aixin Ni, Xintong Han, Adamu Mani Isa, Jingwei Yuan, Jilan Chen, Yanyan Sun","doi":"10.1016/j.psj.2025.105642","DOIUrl":"10.1016/j.psj.2025.105642","url":null,"abstract":"<p><p>Sperm motility is crucial for poultry reproductive efficiency. Seminal plasma is an essential component of semen, and its constituents are linked to sperm motility. This study investigated metabolomics in chicken seminal plasma associated with sperm motility and explored the fertility-enhancing potential of l-carnitine. Semen samples were collected from 16 individual chickens, including 8 with high sperm motility and 8 with low sperm motility. Each sample was divided into two portions: fresh or incubated at 37°C for 30 minutes. A total of 32 seminal plasma samples were obtained and investigated using a nontargeted metabolomics approach. Results indicated that the differential metabolites could be categorized into carnitine-related compounds, choline-related phospholipids, nucleotide derivatives, amino acid-related metabolites and other lipid mediators. More abundance of choline-related, nucleotide derivatives, and lipid mediators in low sperm motility group and incubated groups were identified. A significant decrease in the short-chain acylcarnitine 2-methylbutyroylcarnitine was associated with low sperm motility. Meanwhile, medium- and long-chain acyl-carnitines were elevated in incubated seminal plasma compared to fresh samples. Since acyl-carnitines are formed from carnitine and fatty acids, the potential function of l-carnitine in semen was further determined. An artificial insemination was performed using semen samples either fresh or incubated, and with or without additional of l-carnitine. After in-vitro incubation, samples supplemented with l-carnitine showed significantly improved sperm fertilizing capacity than the unsupplemented group. In conclusion, this study concluded that carnitine compounds play a crucial role in sperm motility, and l-carnitine supplementation enhanced sperm fertility and prolong sperm viability during in vitro storage. These findings suggest potential benefits for both rooster breeding and artificial insemination practices.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105642"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12356462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary DL-methionine supplementation improves growth performance of Linwu ducks by regulating redox state and intestinal mucosal morphology.","authors":"Biguang Lv, Simin Peng, Cong Li, Jingmeng Zhang, Ke Jin, Xin Wang, Xinyu Lei, Xuan Cheng, Fengming Chen, Yang Liu, Guitao Jiang, De-Xing Hou, Xi He, Qiuzhong Dai, Qian Lin","doi":"10.1016/j.psj.2025.105604","DOIUrl":"10.1016/j.psj.2025.105604","url":null,"abstract":"<p><p>This study was designed to investigate the effects of dietary supplementation with different levels of DL-methionine (DL-Met) on growth performance, intestinal mucosal morphology and antioxidant capacity of Linwu ducks. The experiment was conducted to classify 330 healthy 49 days of age female Linwu ducks into 5 treatment groups with 6 replicates per treatment and fed diets with DL-Met levels of 2.5 g/kg, 3.0 g/kg, 3.5 g/kg, 4.0 g/kg, and 4.5 g/kg, respectively, for 21 days. The results showed that compared to the 2.5 g/kg group, the average daily weight gain (ADG) of Linwu ducks in the 3.5 g/kg group was significantly higher (P < 0.05), while the feed-to-gain ratio (FCR) of ducks in the 4.0 g/kg and 3.5 g/kg groups was similarly significantly decreased (P < 0.05). Additionally, the duodenal, jejunal, and ileum mucosal morphology was more structurally intact in the 3.5 g/kg and 4.0 g/kg groups, and the ratio of villus height to crypt depth (V/C) was significantly improved in the duodenum and ileum (P < 0.01). On the other hand, serum triglyceride (TG) and uric acid (BUN) levels were significantly (P < 0.05) decreased in the 3.5 g/kg group. Moreover, serum malondialdehyde (MDA) levels were significantly decreased (P < 0.05) in the 3.5 g/kg and 4.0 g/kg groups, while hepatic glutathione peroxidase (GPX) activity, GPX1, and glutathione S-transferase ɑ3 (GSTα3) mRNA expression levels were significantly increased (P < 0.05). Furthermore, hepatic total antioxidant capacity (T-AOC) activity was also significantly (P < 0.05) increased in the 3.5 g/kg group. In conclusion, dietary DL-Met supplementation could improve the growth performance of Linwu ducks by improving their redox status and intestinal mucosal morphology. Under the conditions of this experiment, the appropriate level of dietary Met supplementation for 49-70 days of age Linwu ducks is between 3.5 g/kg and 4.0 g/kg.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105604"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and computer-assisted validation of a radio frequency identification system for tracking individual chicken visits to functional areas.","authors":"Serge Alindekon, T Bas Rodenburg, Jan Langbein, Birger Puppe, Olaf Wilmsmeier, Sebastian Wille, Helen Louton","doi":"10.1016/j.psj.2025.105627","DOIUrl":"10.1016/j.psj.2025.105627","url":null,"abstract":"<p><p>Understanding how laying hens interact with functional resources-such as drinkers, feeders, perches, nest boxes, and wintergardens-is essential for meeting their physiological needs and enabling species-specific behaviors. This knowledge is crucial for poultry welfare assessments and precision livestock management. However, traditional ethological data collection methods, including direct observation and manual video analysis, are labor-intensive, prone to observer bias, and impractical for individual-level tracking. To overcome these challenges, we developed and validated an RFID-based system for automated, non-invasive tracking of individual hens' visits to key resources, using an established ArUco-based video annotation system as the reference standard. For validation, twenty-one laying hens were fitted with RFID leg bands and 3D-ArUco markers and monitored over five days in a mobile barn setup equipped with ultra-high-frequency RFID antennas. Alignment between data from the RFID and 3D-ArUco systems allowed calculation of performance metrics such as the F1-score-defined as the harmonic mean of precision and sensitivity-for visit durations and event detections (i.e., entries and exits), and the coefficient of determination (r²) for visit counts. Wintergarden showed the highest performance (84 % F1-score, 93 % r²). Metal perch achieved F1-scores of 79 % and 86 % for access and leaving events. Nest boxes showed intermediate performance (78 % F1-score, 77 % r²), while drinkers and feeders were lower (64 % F1-score each; r² values of 69 % and 49 %). These findings confirm RFID's potential for tracking visits to wintergardens, perches, and nest boxes-demonstrating sufficient performance for practical use, though further optimization through antenna positioning remains possible. For feeders and drinkers, however, accurate tracking remains challenging, and complementary technologies may be required, as rapid movements reduce tag dwell time, overcrowding causes signal interference, and open areas increase misreads from nearby surrounding movement. This study highlights RFID's value for behavioral research at the individual level in poultry and supports research-driven innovation in housing equipment design. It also demonstrates how a computer-assisted approach can facilitate validation across diverse behavioral contexts.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105627"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of phloretin on antioxidant status, inflammatory cytokines, blood and biochemical indices, cecal microbial load, and growth performance in broiler chickens subjected to pulmonary arterial hypertension.","authors":"Mokhtar Fathi, Kianoosh Zarrinkavyani, Zahra Biranvand, Morteza Aghil Al Abd","doi":"10.1016/j.psj.2025.105265","DOIUrl":"10.1016/j.psj.2025.105265","url":null,"abstract":"<p><p>This experiment was conducted to investigate the effects of dietary phloretin on broiler chickens under pulmonary arterial hypertension (PAH) conditions. A total of 500 one-day-old male broiler chickens were randomly allocated into five treatments with five replicate pens containing 20 birds per pen. The negative control group was reared at normal temperature and fed with basal diet while the PAH-treatments (positive control, F-200, F-400, and F-600) were exposed to low temperatures and received drinking water supplemented with excess salt to induce PAH and fed with basal diet containing 0, 200, 400, and 600 mg of phloretin per kilogram, respectively. Blood samples were collected from broilers at 42 days of age. Results showed that PAH decreased body weight gain (BWG), antioxidant capacity, immunoglobulin G (IgG), hematological indices, and cecal lactic acid bacteria population (CLBP), but increased feed intake (FI), PAH-related mortality, feed conversion ratio (FCR), PAH index, and pro-inflammatory cytokines, alanine transaminase (ALT), aspartate transaminase (AST), triglyceride (TG), and total cholesterol (TC) contents in serum (P<0.001). Among the PAH treatments, supplementary phloretin improved growth performance indices and reduced PAH index, and PAH-related mortality (P < 0.01). The activities of superoxide dismutase, catalase, and glutathione peroxidase, and IgG, and IgM in serum were increased, and malondialdehyde was reduced by phloretin supplementation (P <0.01). The PAH-induced effect on contents of pro-inflammatory cytokines were reduced by dietary phloretin supplementation (P <0.01). Moreover, supplementation of diets with phloretin alleviated the adverse effect of PAH as reflected by a reduction in ALT, AST, TG, and TC (P <0.01). The red blood cell count, hemoglobin, hematocrit, and heterophil levels reduced by phloretin supplementation. Phloretin increased the population of CLBP. In conclusion, phloretin supplementation during PAH may mitigate PAH-associated physiological and biochemical alterations in broiler chickens.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 10","pages":"105265"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the B-cell immune landscape in duck hepatitis A virus type 3 through single-cell genomics.","authors":"Saisai Zhao, Yaru Fan, Dalin He, Mian Wu, Xinyuan Xu, Xinhong Man, Siming Zhu, Ruiqi Shi, Yafei Qin, HongYu Ma, Qiang Feng, Yi Tang, Youxiang Diao","doi":"10.1016/j.psj.2025.105929","DOIUrl":"https://doi.org/10.1016/j.psj.2025.105929","url":null,"abstract":"<p><p>Antibodies protect the organism against many diseases. How the protective antibody memory response against Duck Viral Hepatitis type 3 (DHAV-3) activates transcriptional programs, cell proliferation, and antigen-specific antibody production is unknown. To explore the mechanisms of DHAV-3 immunity and identify potential therapeutic targets, we performed single-cell RNA sequencing (scRNA-seq) and B cell receptor (BCR) library analyses to characterize the cellular landscape of splenic B-cells from mice immunized with DHAV-3. We observed significant changes in the number and proportion of B cell populations induced by DHAV-3. Through integrating single-cell gene expression profiling and BCR analysis, we have creatively identified a novel IGHV1 germline clone, IGHV1-20, which has not been previously described in the literature. Interestingly, a trend of enhanced cellular communication was observed between a novel B-cell subset, Gm37915+ B cells, and Nuggc+ B cells. Furthermore, we identified two specifically upregulated integrin subunits, ITGAL and ITGB2, in the experimental group (EG), which possibly correlated with the activation status of B cells and enhanced immune-cell interactions. Our findings not only shed light on the immunological characteristics of splenic B cells in the context of DHAV-3 immunity but also provide important advances in our understanding of targeted antibodies against this virus.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 12","pages":"105929"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}