Poultry Science最新文献

{"title":"First establishment of a Duck Model for in vivo and in vitro studies of West nile virus (Kunjin subtype)","authors":"Zhen Wu ,&nbsp;Tao Hu ,&nbsp;Zhou Zhou ,&nbsp;Yu He ,&nbsp;Tao Wang ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Dekang Zhu ,&nbsp;Mafeng Liu ,&nbsp;Xinxin Zhao ,&nbsp;Qiao Yang ,&nbsp;Ying Wu ,&nbsp;Shaqiu Zhang ,&nbsp;Juan Huang ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Bin Tian ,&nbsp;Anchun Cheng ,&nbsp;Shun Chen","doi":"10.1016/j.psj.2025.105214","DOIUrl":"10.1016/j.psj.2025.105214","url":null,"abstract":"<div><div>We present a method for constructing a Kunjin virus (KUNV) infectious clone capable of stable proliferation in bacterial systems. This KUNV infectious clone successfully produces infectious viral particles within host cells. In vitro, the recombinant virus demonstrates the ability to replicate in primary duck cells and induces 100 % mortality in duck embryos. In vivo, the recombinant virus exhibits virulence in our established duckling model, leading to clinical signs such as hepatic haemorrhage, splenomegaly, and pancreatic haemorrhage. Furthermore, the recombinant rKUNV induced a mortality rate of 20 % in ducklings, thereby providing a robust model for studying the virus’s pathogenicity in horses or human. To expand upon this foundation, we have developed KUNV replicons tagged with luciferase and mCherry, which confirm successful KUNV replication in primary duck cells and hold potential for future antiviral drug screening. The KUNV reverse genetics system, alongside the in vitro and in vivo duck infection models, offers a valuable platform for identifying pathogenic factors relevant to KUNV infection in humans, horses, and avian species. Additionally, this system could be instrumental in advancing vaccine development and antiviral drug research against KUNV.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105214"},"PeriodicalIF":3.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary valine affects Japanese quails’ sperm parameters and testis histology","authors":"Zahra Dinari ,&nbsp;Abouzar Najafi ,&nbsp;Seyed Davood Sharifi ,&nbsp;Leila Rashki Ghaleno ,&nbsp;AliReza Alizadeh ,&nbsp;Morteza Pashaei ,&nbsp;Ali Rashidi","doi":"10.1016/j.psj.2025.105181","DOIUrl":"10.1016/j.psj.2025.105181","url":null,"abstract":"<div><div>Valine is an essential amino acid that participates in various physiological and metabolic activities. The objective of the present study was to investigate the influence of different valine levels in the diet on the reproductive performance of adult male Japanese quails. Forty male quail chicks (14-24 week old) were employed in a completely randomized design with 5 treatments (0.65, 0.75, 0.85, 0.95, and 1.05 % valine in the diet), 4 replicates and 2 birds in each replicate over a period of 35 days following a 14-day adaptation period. Semen was collected weekly, and its characteristics were analyzed. The birds were then ethically slaughtered, and histological analyses were performed on testicular samples. Results showed that the different valine levels had no significant effect on testicle volume, length and relative weight, although testicle diameter tended to decrease with increasing valine level in the diet in a linear and quadratic manner (p &lt; 0.05). The total motility and progressive motility of sperm decreased with an increase in dietary valine (p &lt; 0.05). Abnormal sperm morphology increased with increasing valine level in the diet and the difference between treatments was significant. Different valine levels in the diet significantly affected the height of seminiferous tubule epithelia, diameter and number of tubules as well as the tubular differentiation index of the testis; but the spermatogenic index was not affected by the treatments. The findings of the study indicate that when valine levels in the diet exceed 0.75 %, there is an adverse impact on testicular volume and diameter, as well as the qualitative properties of sperm in adult quails. It is advisable to pursue additional research in this area.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105181"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated 16S rRNA and metagenomic sequencing reveals the distribution of key antibiotic resistance genes in duck gut microbiota","authors":"Hongbo Zeng ,&nbsp;Hua Yang ,&nbsp;Zixian Fu ,&nbsp;Lingyan Ma ,&nbsp;Lizhi Lu ,&nbsp;Tao Zeng ,&nbsp;Yingping Xiao ,&nbsp;Wentao Lyu","doi":"10.1016/j.psj.2025.105206","DOIUrl":"10.1016/j.psj.2025.105206","url":null,"abstract":"<div><div>The duck gut microbiota is essential for host health and is considered a potential reservoir for antibiotic resistance genes (ARGs). However, research on ARGs in the duck gut microbiota is limited. This study collected 120 intestinal content samples from five segments (duodenum, jejunum, ileum, cecum, and colorectum) of ducks raised under two rearing conditions (with or without an open-air swimming pool). We compiled a comprehensive inventory of microbial genes in the duck gut and conducted an analysis of microbial composition and function across all intestinal segments using 16S rRNA gene sequencing combined with metagenomics. The findings revealed that Firmicutes were the most prevalent microbes in all intestinal segments. In the foregut (duodenum, jejunum, and ileum), microbial functions were mainly related to genetic information processing such as transcription, translation, replication, and glycosynthesis/gluconeogenesis. Conversely, in the hindgut (cecum and colorectum), microbial functions were primarily associated with the biosynthesis of secondary metabolites and various metabolic pathways. The analysis of ARGs indicated a higher relative abundance of ARGs in the cecum and colorectum (<em>P</em> &lt; 0.05) of ducks in the presence of an open-air swimming pool compared to the absence of one. Furthermore, through co-occurrence network analysis, we identified <em>Bacteroides, Roseburia, Ruminococcus</em>, and <em>Blautia</em> as potential hosts of ARGs such as <em>tetQ, tet32, tet37, vanR, vanG</em>, and <em>acrB</em> in the hindgut. This study provides new insights into the complex relationship between ARGs and the microbial community in duck intestines, laying a theoretical groundwork for understanding the transmission dynamics of ARGs in these ecosystems.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105206"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic transcriptomic and metabolomic analyses in Zi geese ovaries with different clutch lengths","authors":"Shengjun Liu ,&nbsp;Jiaxin Yin ,&nbsp;Kexin Cong ,&nbsp;Shan Yue ,&nbsp;Yuanliang Zhang ,&nbsp;Jinyan Sun ,&nbsp;Xiaofang Ren ,&nbsp;Ke Jiang ,&nbsp;Yunuo Liu ,&nbsp;Xiuhua Zhao","doi":"10.1016/j.psj.2025.105210","DOIUrl":"10.1016/j.psj.2025.105210","url":null,"abstract":"<div><div>The clutch is defined as consecutive days of oviposition. Clutch length is an index that reflects ovulation persistence, and is highly correlated with egg production in birds. To identify the genetic markers associated with clutch length in geese, two consecutive experiments were conducted. In the first experiment, 200 Zi geese were selected, all 230 days old, were selected from the same batch and raised individually in the same environment. Data of egg-laying and clutch traits were recorded. After the laying period, three geese with the longest clutch lengths were selected to form the length clutch group (<strong>LCG</strong>) and three geese with the shortest clutch lengths were formed the short clutch group (<strong>SCG</strong>). In the second experiment, the ovaries of six geese were collected for transcriptomic and metabolomic analyses. The results showed that large clutch length (<strong>LCL</strong>) and average clutch length (<strong>ACL</strong>) were positively correlated with egg number (<strong>EN</strong>) (<em>P</em> &lt; 0.01; <em>r</em> = 0.63 and 0.60, respectively). Large clutch number (<strong>LCN</strong>) was significantly correlated with the peak egg number (<strong>PEN</strong>) (<em>r</em> = 0.58, <em>P</em> &lt; 0.01) and EN (<em>r</em> = 0.60, <em>P</em> &lt; 0.01). EN, LCN, LCL, and ACL showed significant differences (<em>P</em> &lt; 0.01) between the two clutch length groups. Transcriptomic analysis identified 424 differentially expressed genes (<strong>DEGs</strong>). Functional enrichment analysis revealed that these DEGs were mainly involved in neuroactive ligand-receptor interactions, ovarian steroidogenesis, and calcium signaling pathways. Further, <em>AVPR1A, FGF14</em>, and <em>LHCGR</em> were predicted as the key genes regulating LCL. Metabolomic analysis identified 349 differential metabolites (<strong>DMs</strong>) in both the positive and negative ion modes. Pyruvate, isocitric acid, D/L‑serine, 3-phospho-<span>d</span>-glycerate, succinate, glycine, and glutamic acid were identified as the key metabolites mainly enriched in the signaling pathways of the TCA cycle. Integration of transcriptomic and metabolomic data revealed critical gene-metabolite pairs, including <em>ACSL4</em>–phosphoenolpyruvate, implicated in LCL regulation. In summary, this study provides new insights into the genes and molecular markers affecting LCL in Zi geese.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105210"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Nutritional supplementation of breeding hens may promote embryonic development through the growth hormone-insulin like growth factor axis” [POULTRY SCIENCE 2024 Aug;103(8):103945./ 38905758]","authors":"Guoxia Wang,&nbsp;Haochu Deng,&nbsp;Taiping Wang,&nbsp;Xin Zheng","doi":"10.1016/j.psj.2025.105148","DOIUrl":"10.1016/j.psj.2025.105148","url":null,"abstract":"","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105148"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of inclusion of black soldier fly larvae on growth performance, relative organ weight, and meat quality of broiler chickens","authors":"H.N. Lee ,&nbsp;K.H. Yum ,&nbsp;G.L. Yeom ,&nbsp;Y.B. Kim ,&nbsp;J.Y. Park ,&nbsp;S. Park ,&nbsp;G. Park ,&nbsp;Y. Choi ,&nbsp;J. Choi ,&nbsp;J.H. Kim","doi":"10.1016/j.psj.2025.105208","DOIUrl":"10.1016/j.psj.2025.105208","url":null,"abstract":"<div><div>The objective of this experiment was to investigates effects of inclusion of black soldier fly larvae (BSFL) on growth performance, relative organ weight, and meat quality of broiler chickens. A total of 180 1-<span>d</span>-old broiler chickens were randomly allotted to 1 of 3 dietary treatments with 5 replicates. Each replicate consisted of 12 birds. Experimental diets were formulated to contain full-fat BSFL at inclusion levels of 0 %, 1 %, and 2 %. These diets were provided on an ad libitum basis for 5 wk. Results indicated that birds fed diet containing 1 % BSFL had greater (<em>P</em> &lt; 0.05) feed efficiency than those fed diets containing 2 % BSFL. However, BW gain and feed intake of broiler chickens were not affected by increasing inclusion levels of BSFL in diets. Increasing inclusion levels of BSFL in diets showed a quadratic relationship (<em>P</em> &lt; 0.05<em>)</em> with relative thymus weight. For meat color, values for redness (a*) and yellowness (b*) were decreased (linear, <em>P</em> &lt; 0.05) as BSFL inclusion level of diets increased. Thiobarbituric acid reactive substance decreased (quadratic, <em>P</em> &lt; 0.05) with increasing inclusion levels of BSFL in diets. Melanin concentrations in breast meat and liver characteristics were not affected by increasing inclusion levels of BSFL in diets. Increasing concentrations of BSFL in diets increased myristic acid concentrations (linear, <em>P</em> &lt; 0.01) and eicosapentaenoic acid concentrations (linear, <em>P</em> &lt; 0.05) in breast meat. These results suggest that dietary supplementation of BSFL has positive effects on immune organ weight, breast meat quality, and fatty acid compositions in breast meat. Therefore, BSFL can be used as a feed ingredient for broiler chickens. Also, we suggest that dietary supplementation of 1 % BSFL improves the performance and health of broiler chickens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105208"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and regulation of adrenomedullin 2 gene in ovarian follicles and its effect on follicular granulosa cells in chicken","authors":"Lihong Li ,&nbsp;Dandan Li ,&nbsp;Qingqing Wei ,&nbsp;Li Kang ,&nbsp;Yi Sun ,&nbsp;Yunliang Jiang","doi":"10.1016/j.psj.2025.105204","DOIUrl":"10.1016/j.psj.2025.105204","url":null,"abstract":"<div><div>Adrenomedullin 2 (<strong>ADM2</strong>) is a member of the calcitonin/calcitonin gene-related peptide family and is expressed in various tissues including uterus and ovary. ADM2 is reported to play an important role in ovarian follicle development and steroidogenesis in mammals. In this study, the mRNA expression, the effects of key reproductive hormones on its expression and its effect on follicular granulosa cells were investigated. We found that the expression of <em>ADM2</em> mRNA was dynamic during follicle development and was lower in the granulosa and theca cells after follicle selection. After treatment with follicle stimulating hormone (<strong>FSH</strong>), the expression of <em>ADM2</em> mRNA decreased in the theca cells of pre-hierarchical follicles (<strong>Pre-TCs</strong>) and hierarchical follicles (<strong>Post-TCs</strong>), but increased in the granulosa cells of pre-hierarchical follicles (<strong>Pre-GCs</strong>) and hierarchical follicles (<strong>Post-GCs</strong>). After treatment with progesterone (<strong>P4</strong>), the expression of <em>ADM2</em> mRNA decreased in Pre-TCs, Pre-GCs and Post-GCs, but increased in Post-TCs. After treatment with estradiol (<strong>E2</strong>), the expression of <em>ADM2</em> mRNA increased in Post-TCs and Post-GCs. <em>ADM2</em> suppressed the viability and proliferation of both Pre-GCs and Post-GCs, while facilitated their apoptosis. In both Pre-GCs and Post-GCs, <em>ADM2</em> promoted the expression of genes involved in P4 synthesis (steroidogenic acute regulatory protein, <strong><em>StAR</em></strong>; cytochrome P450 family 11 subfamily A member 1, <strong><em>CYP11A1</em></strong>; 3β-hydroxysteroid dehydrogenase, <strong><em>3β-HSD</em></strong>) and P4 secretion. These data suggest that <em>ADM2</em> plays an important role by decreasing the proliferation and enhancing P4 secretion of granulosa cells around chicken follicle selection.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105204"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chickens perceive humans as social buffers and may follow human-given cues: A pilot study","authors":"Vitor Hugo Bessa Ferreira ,&nbsp;Elise Calesse ,&nbsp;Lucille Dumontier ,&nbsp;Fabien Cornilleau ,&nbsp;Julie Lemarchand ,&nbsp;Auriane Foreau ,&nbsp;Maxime Quentin ,&nbsp;Léa Lansade ,&nbsp;Céline Tallet ,&nbsp;Xavier Boivin ,&nbsp;Ludovic Calandreau","doi":"10.1016/j.psj.2025.105203","DOIUrl":"10.1016/j.psj.2025.105203","url":null,"abstract":"<div><div>Positive perception of humans, extensively documented in domestic mammals, remains comparatively underexplored in domestic birds like chickens, with existing studies largely focusing on fear reduction. This research evaluated whether chickens perceive humans positively, accounting for interaction types and breed differences. Two breeds (Lohmann LSL Classic, Brown Classic) experienced physical contact (PC), visual-only contact (VC), or minimal human contact (MC) over 13 days (Days 35–51; PC and VC: 1–2 min/day). Birds were assessed using three behavioral tests. During the separation–reunion test (Days 52–53), individuals underwent two 3-min separations (experimenter absent) and reunions (experimenter present) in an open-field setting. Subsequently, the experimenter attempted up to six standardized capture attempts to catch the birds (the capture test). Lastly, the local enhancement test (Days 120–137) assessed birds' ability to use human presence/gestures to locate food. In the separation-reunion test, PC birds exhibited calmer/positive behaviors, such as reduced vigilance and increased foraging, in the presence of the experimenter compared to being alone. Conversely, MC birds displayed fewer calm behaviors and greater withdrawal when the experimenter was present. Brown birds showed more calm behaviors, reduced movement, and spent more time near the experimenter than white birds. The capture test supported these results, with PC and brown birds being easier to capture. In the local enhancement test, two PC individuals and the brown PC group as a whole successfully used human-given cues to locate food rewards. These results indicate that, as observed in other species, chickens—especially those with positive human experiences—can associate humans with rewarding outcomes. Positive interactions may also lead chickens to perceive humans as social buffers—that is, as factors that help mitigate stress in challenging situations. Although fundamental, this study highlights the potential of breed-sensitive approaches to improve poultry welfare and opens the discussion on adapting human–animal interactions to breed-specific characteristics. These insights can inform welfare-enhancing practices and provide practical tools for on-farm management that benefit both animals and farmers.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105203"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Compound-specific isotopic analysis in 34S-labelled chicken tissues using high resolution gas chromatography/mass spectrometry","authors":"Julie Lalande ,&nbsp;Jean-Baptiste Domergue ,&nbsp;Yves Mercier ,&nbsp;Francis A. Eugenio ,&nbsp;Gonzalo Cantalapiedra-Hijar ,&nbsp;Sophie Tesseraud ,&nbsp;Guillaume Tcherkez","doi":"10.1016/j.psj.2025.105162","DOIUrl":"10.1016/j.psj.2025.105162","url":null,"abstract":"<div><div>Many food supplements include sulphur (S)-containing additives such as methionine or synthetic compounds like 2‑hydroxy-<em>S</em>-methyl-thiobutyric acid (HMTBA). However, monitoring the metabolic use efficiency of S-containing additive is challenging, and requires specific methods, based on isotopic labelling. The most direct route is the utilisation of ³⁴S-enriched material and subsequent measurement of ³⁴S-abundance in tissues. While this can be carried out routinely using elemental analysis coupled with isotope ratio mass spectrometry (IRMS) for bulk S from raw tissue material, there is currently no IRMS-based method adapted to compound-specific isotopic analysis for sulphur. Here, we present a method based on gas chromatography coupled to high resolution mass spectrometry (GC-MS) to measure ³⁴S-abundance in both free and protein-bound S containing amino acids. This method elaborates on metabolomics based on GC-MS analysis of trimethylsilylated extracts. Specific ion fragments comprising a sulphur atom could be identified and their isotopic pattern was used to compute % ³⁴S. The high resolution was useful to avoid the confounding effect of natural carbon (¹³C₂) or (¹⁸O) isotopologues but required a correction for silicium (Si) isotope because the mass excess of ³⁰Si (+ 1.9968 a.m.u.) was close to that of ³⁴S (+ 1.9957 a.m.u.) and therefore the ³⁰Si and ³⁴S isotopologues could not be separated. This technique was applied to broilers fed with ³⁴S-labelled methionine and showed that ³⁴S could be easily traced in different organs. ³⁴S-methionine redistributed mostly to homocysteine with little ³⁴S-enrichment in cysteine and taurine, due to the isotopic dilution by food cysteine supply. The results show that our method can be implemented to follow the metabolic incorporation of S-containing additives such as methionine in broilers.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105162"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Music intervention mitigates LPS-induced gut barrier disruption and immune stress in broilers via TLR4/NF-κB regulation","authors":"Shengzi Jin ,&nbsp;Haowen Wang ,&nbsp;Haiyue Gong ,&nbsp;Lu Guo ,&nbsp;Haoran Zhang ,&nbsp;Jiaqi Zhang ,&nbsp;Qingqing Chang ,&nbsp;Jianhong Li ,&nbsp;Runxiang Zhang ,&nbsp;Jun Bao","doi":"10.1016/j.psj.2025.105189","DOIUrl":"10.1016/j.psj.2025.105189","url":null,"abstract":"<div><div>Immune stress induced by harsh environment in intensive farming can impair broiler intestinal health. Although music as an environmental intervention can alleviate short-term stress injury, its long-term regulatory mechanism on intestinal inflammation has not been clarified. In this study, we investigated the effects of a music-enriched environment on growth performance, intestinal barrier function, and inflammatory responses in lipopolysaccharide (LPS)-induced immunostressed broilers. AA broilers were randomly divided into four groups: control group (<strong>CON</strong>), music-enriched environment group (<strong>MUC</strong>), LPS-induced immune stress group (<strong>LPS</strong>) and music-enriched environment + LPS group (<strong>MUC+LPS</strong>). On the 14th, 16th and 18th days, the LPS and MUC+LPS groups were injected intraperitoneally with 500 μg of LPS to construct an immune stress model, and the CON and MUC groups were injected with an equal amount of saline. On day 28, the birds were sacrificed to detect the indicators associated with intestinal barrier and inflammation. The LPS group showed a significant decrease in performance from 14 to 28 days, with elevated serum levels of CORT, ACTH, DAO, and <span>d</span>-LA, and a decrease in the activity of intestinal mucosal SOD/GSH-Px, and impaired gut morphology. impaired; music remission significantly alleviated the decline in production performance, reduced the levels of stress hormones and markers of intestinal barrier damage, while elevating jejuno-ileal GSH-Px activity and improving intestinal morphology. Significant inflammatory gene expression characteristics were observed in jejunum and ileum tissues after LPS injection: upregulation of TLR4, NF-κB, TNF-α, IL-1β, and IL-6, and significant suppression of jejunal IL-10 expression. Notably, IL-10 and IFN-γ expression in the ileum did not show statistical differences. Inflammation-related gene expression showed an overall down-regulation trend after the music intervention, but was still significantly different from the control group. Music intervention on the regulation of jejunal MYD88 and ileal TNF-α - the LPS group did not show statistically significant differences in the expression of these two key inflammatory nodes with the LPS+MUS group. Mechanistic studies have shown that LPS triggers an oxidative stress cascade through activation of the TLR4/NF-κB signaling axis, leading to disruption of intestinal barrier integrity. In contrast, music exposure exerts a protective effect through a dual mechanism: on the one hand, it helps to enhance the expression of the tight junction protein ZO-1/Occludin to repair the physical barrier; on the other hand, it inhibits the activation of the TLR4/NF-κB pathway, which can effectively alleviate LPS-induced immunopathological damage.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105189"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}