Poultry Science最新文献

{"title":"Synergistic effects of copper and bile acids on cholesterol reduction in eggs of laying hens","authors":"Han Chen,&nbsp;Yifan Li,&nbsp;Shoukang Cao,&nbsp;Mingyang Wang,&nbsp;Yuqing Mu,&nbsp;Xuezhuang Wu","doi":"10.1016/j.psj.2025.105202","DOIUrl":"10.1016/j.psj.2025.105202","url":null,"abstract":"<div><div>This study investigates the combined effects of copper (Cu) and bile acids (BA) on production performance, lipid metabolism, and antioxidant capacity in laying hens. During a 67-day trial, a total of 300 Jingfen 8 laying hens aged 160 days were randomly allocated into four treatment groups: Control (basal diet), Cu (25 mg/kg Cu), BA (500 mg/kg BA), and Mix (a combination of 25 mg/kg Cu and 500 mg/kg BA). The results indicated that the Mix group experienced a significant decrease in egg cholesterol levels, particularly in the yolk cholesterol content, along with an enhancement in the total antioxidant capacity. Additionally, the liver cholesterol metabolism was modulated by upregulation of CYP7A1 and ABCG5, alongside downregulation of SREBP-2 and HMGCR. While Cu and BA individually showed minimal effects on production, their combination yielded a synergistic impact on lipid metabolism and antioxidant defense. However, the Mix group showed reduced liver zinc content, suggesting a Antagonistic relationship between Cu and Zn. These findings suggest that Cu and BA supplementation may enhance egg quality and metabolic health in laying hens, highlighting the importance of maintaining balanced trace element ratios in poultry diets.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105202"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing high school students' interest, motivation, and knowledge through poultry science workshops","authors":"Elizabeth L. Karcher,&nbsp;Dea M. Bogdanic,&nbsp;Sara E. Cloft","doi":"10.1016/j.psj.2025.105200","DOIUrl":"10.1016/j.psj.2025.105200","url":null,"abstract":"<div><div>There is a general lack of interest among undergraduate students in pursuing careers in the poultry industry. Although currently limited, more opportunities to enhance understanding and awareness of the industry should be made available to secondary school students. This study evaluated the impact of short-term poultry science workshops on high school students’ situational interest, motivation, and knowledge. A total of 45 students participated in one of five 90-minute workshops, each consisting of four stations focused on poultry science and animal health. Pre- and post-assessments were administered to measure student interest in the poultry industry, motivation, and content knowledge. Overall, content knowledge increased as a result of participation. Additionally, participants exhibited high levels of intrinsic motivation and identified regulation. Participants with 4-H experience reported lower amotivation compared to non-4-H participants. These findings emphasize the importance of adapting agricultural education to diverse backgrounds, focusing on relevance to increase engagement and maximize learning outcomes.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105200"},"PeriodicalIF":3.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD","authors":"Hangbo Yu ,&nbsp;Guodong Wang ,&nbsp;Wenying Zhang ,&nbsp;Ziwen Wu ,&nbsp;Xinxin Niu ,&nbsp;Mengmeng Huang ,&nbsp;Yulong Zhang ,&nbsp;Runhang Liu ,&nbsp;Jinze Han ,&nbsp;Mengmeng Xu ,&nbsp;Jingzhe Han ,&nbsp;Dan Ling ,&nbsp;Erjing Ke ,&nbsp;Suyan Wang ,&nbsp;Hongyu Cui ,&nbsp;Yanping Zhang ,&nbsp;Yuntong Chen ,&nbsp;Yongzhen Liu ,&nbsp;Yulu Duan ,&nbsp;Yulong Gao ,&nbsp;Xiaole Qi","doi":"10.1016/j.psj.2025.105195","DOIUrl":"10.1016/j.psj.2025.105195","url":null,"abstract":"<div><div>Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), threatens the global poultry industry with acute immunosuppression. The non-lethal novel variant IBDV (nVarIBDV) has been identified as the primary cause of a new outbreak of atypical IBD in China. However, since 2023, several poultry farms in China have continuously reported the detection of vvIBDV in chicken flocks exhibiting atypical IBD characteristics. To analyze this abnormal problem, the prevalence of IBDV in major poultry farming areas in China from 2023 to 2024 was monitored. Our epidemiological survey revealed four coexisting IBDV strains in China, nVarIBDV (69.9 %, 65/93) ranked the first, followed by vvIBDV (16.1 %, 15/93), attIBDV (8.6 %, 8/93), and cIBDV (5.4 %, 5/93). Notably, all these 15 vvIBDV strains detected in this study harbored single or combination mutations in the VP2 hypervariable region (HVR) including residue 279: D279N (seven strains), G254D/I256L/D279N (one strain), or A222T/G254D/I256L/D279N (seven strains). Furthermore, one representative strain of IBDV-NMG23-1701 which contained the D279N mutation was isolated. In the phylogenetic tree constructed based on the IBDV genome, IBDV-NMG23-1701 was located in the vvIBDV branch. However, IBDV-NMG23-1701 did not kill chickens directly but induced severe subclinical symptoms of atypical IBD. We named these strains mutated vvIBDV (mvvIBDV), which genotypically belonged to vvIBDV but exhibited atypical IBD symptoms. The mvvIBDV has been detected in several major poultry farming areas in China. This is the first study to identify mvvIBDV as a new pathogen causing atypical IBD, providing a scientific basis for optimizing prevention and control strategies.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105195"},"PeriodicalIF":3.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis of goose epididymis reveals molecular markers associated with sperm mobility","authors":"Xiaofang Ren ,&nbsp;Ke Jiang ,&nbsp;Jiaxin Yin ,&nbsp;Zhigang Ma ,&nbsp;Zhifeng Chen ,&nbsp;Kun Yang ,&nbsp;Shengjun Liu","doi":"10.1016/j.psj.2025.105180","DOIUrl":"10.1016/j.psj.2025.105180","url":null,"abstract":"<div><div>The low fertility of geese has long constrained the development of the geese industry. Sperm quality plays a critical role in fertility, and sperm mobility (<strong>SM</strong>) serves as a key indicator of sperm quality. However, the molecular mechanisms underlying SM remain largely unexplored. The objective of this study was to identify molecular markers associated with SM in the epididymis of Zi geese (Anser cygnoides L.). The SM of 40 one-year-old ganders was assessed. Based on SM values, six ganders were selected: three with the highest SM (H group: <em>n</em> = 3, SM = 0.43 ± 0.02) and three with the lowest SM (L group: <em>n</em> = 3, SM = 0.10 ± 0.01, <em>P</em> &lt; 0.001). Semen quality parameters, fertility, and hormone levels were measured in both groups. Epididymal tissues from the six ganders were subjected to transcriptomic and metabolomic analyses. Results identified 438 differentially expressed genes (<strong>DEGs</strong>) between the groups, primarily associated with transmembrane transport of proteins and ions. These DEGs were enriched in pathways such as “alanine, aspartate and glutamate metabolism,” “butanoate metabolism,” and the “PPAR signaling pathway.” Among these, <em>ATP12A, ATP1B4</em>, and <em>CNDP1</em> were identified as key genes regulating SM. Additionally, 486 metabolites showed significant differences between the groups in both positive and negative ion modes. Integration of transcriptomic and metabolomic data revealed critical gene-metabolite pairs, including <em>CNDP1</em>–citric acid, implicated in SM regulation. Notably, the “arginine biosynthesis” pathway was significantly enriched by both DEGs and differential metabolites. In conclusion, this study provides novel insights into the molecular mechanisms regulating SM in the epididymis and lays a theoretical foundation for geese breeding programs.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105180"},"PeriodicalIF":3.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of mediterranean fruit fly rearing residues and biological supplementation on performance of gimmizah chicks","authors":"Mahmoud H. Hatab ,&nbsp;Nashaat S. Ibrahim ,&nbsp;Waheed A.A. Sayed ,&nbsp;Aml M.M. Badran ,&nbsp;Birgit A. Rumpold","doi":"10.1016/j.psj.2025.105198","DOIUrl":"10.1016/j.psj.2025.105198","url":null,"abstract":"<div><div>For a transformation of the global food system towards sustainability, circular approaches and nutrient-rich side-stream valorization are mandatory. Moreover, affordable and sustainable alternatives to corn, soy, and fish meal are needed in poultry production. Recently, insects and their derived products have gained research interest as alternative sources of conventional feed ingredients in poultry nutrition. The Mediterranean fruit fly (medfly; <em>Ceratitis capitata</em>) production industry using sterile insect technology amasses thousands tonnes of rearing residues annually. This study is the first to shed light on the potential use of medfly rearing residues (<strong>MFRR</strong>), as a partial replacement for corn and soybean in Gimmizah chicks’ diets, with or without biological supplementation (<strong>BS</strong>). It evaluates their effects on growth, carcass characteristics, blood indices, serum biochemical and histological changes in internal organs. A 7-week trial was conducted using 240, 15-day-old Gimmizah chicks, which were randomly divided into four groups (6 replicates, 10 birds each): the first group (T1) was fed a corn-soybean control diet, the 2nd group (T2) fed the control diet enriched with 1 ml BS/kg diet. The 3rdgroup (T3) received the control diet after replacing 10 % of corn and soybean with <strong>MFRR</strong> meal, while the 4th group (T4) fed the 10 % replacement by <strong>MFRR</strong> combined with 1 ml of BS. All groups received isoenergetic and isoprotienic diets with free access to feed and water for 49 days trial period. Compared to the control, both BS and <strong>MFRR</strong> inclusion with or without BS (T4 and T3, respectively) positively improved body weight, feed consumption, feed conversion, performance index and carcass yield. Blood analysis showed increased red blood cells, hemoglobin, packed cell volume, total protein, albumin, globulins, triglycerides, cholesterol, thyroxine hormone, uric acid and creatinine, with no adverse histological alteration in the bursa or intestine. In conclusion, the study suggests that MFRR can effectively replace 10 % of traditional feed ingredients, with or without BS, enhancing chicks' performance and health. Further future studies are recommended for broader application of MFRR in poultry nutrition.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105198"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics-based analysis of key genes, metabolites and pathways unveils mechanism associated with social rank in Chickens","authors":"Mengqiao Zhao,&nbsp;Limin Xing,&nbsp;Yushan Li,&nbsp;Jiaxin Zhang,&nbsp;Yinghui Liu,&nbsp;Fei Ye,&nbsp;Siyu Chen","doi":"10.1016/j.psj.2025.105192","DOIUrl":"10.1016/j.psj.2025.105192","url":null,"abstract":"<div><div>Social rank plays a crucial role in shaping physiological, psychological and immune responses in animals. Our previous study found that gut microbes and short-chain fatty acids as well as brain neurotransmitters are involved with the mechanism of social hierarchy. Nevertheless, how these gut and brain metabolites interact to affect the mechanism of social hierarchy in chickens is not yet known. In this study, 40 hens were randomly divided into four groups at the age of 49 days, and social rank of each hen was determined by 6 times according to the feeding competition tests. Then hens ranked 1/2 were named High Social Rank (HSR, n = 8) and hens ranked 9/10 were named Low Social Rank (LSR, n = 8). We use multiomics to explore gut metabolites, neurotransmitters, and brain transcriptome, neurotransmitters and related gene expression in 91-day-old chickens, so as to better understand the underpinning mechanism that regulates the social hierarchy. We found that the pro-inflammatory genes were significantly lower while anti-inflammatory factor in spleen was higher in HSR chickens than in LSR chickens (<em>P</em>&lt;0.05). Besides, seven immune-related genes were significantly different in the amygdala between HSR and LSR (<em>P</em> &lt; 0.05). In addition, <em>AVP, RXFP3, VIP</em> and <em>NKX2-1</em> were associated with the social rank through GABAergic neurons and neuroactive ligand-receptor pathways, with the up-regulation of <em>5-HT</em> in the amygdala of LSR. Genes <em>SLC11A2</em> and <em>HMOX1</em> in ferroptosis pathway influenced the cecum metabolite l-glutamate and tyrosine. While fumaric, l-glutamic and 4-Oxoproline were found enriched in “alanine, aspartate and glutamate metabolism” and “arginine biosynthesis”. In conclusion, social rank affects the immunity, in which higher ranking hens show better anti-inflammatory and lower pro-inflammatory than lower ranking hens. Genes <em>TAP2, PLD4, P2RX7, ALDH9A1, SLC11A2, ADM, C3, AVP, RXFP3, VIP, NKX2-1, SLC11A2</em> and <em>HMOX1</em> may play an important regulatory role on GABAergic neurons, neuroactive ligand-receptor and ferroptosis pathways related to neuron, immune, and stress behaviour, and in turn affects social rank. Fumaric, l-glutamic and 4-Oxoproline, may regulate social rank through the “alanine, aspartate and glutamate metabolism” and “arginine biosynthesis”.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105192"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effect of heat stress on egg production traits in Thai native chickens (Lueng Hang Kao Kabin) as determined by the temperature-humidity index","authors":"Krittaphak Buranawit ,&nbsp;Nalinee Imboonta ,&nbsp;Siriporn Tongsiri ,&nbsp;Yutaka Masuda ,&nbsp;Praopilas Phakdeedindan","doi":"10.1016/j.psj.2025.105196","DOIUrl":"10.1016/j.psj.2025.105196","url":null,"abstract":"<div><div>Heat stress is a considerable challenge for the poultry industry, impacting even native breeds well-known for their adaptability. This study aimed to examine the effect of heat stress, defined as a temperature-humidity index (<strong>THI</strong>), on egg production traits in Thai native chickens (Lueng Hang Kao Kabin breed, <strong>LHKK</strong>). The THI values were calculated using three different equations based on the weather data on the same day (day 0) as the observation and up to seven days (day -7) prior to the observation. The models were preliminarily tested to identify the significant effects on egg production traits based on adjusted R², residual error variance, and Akaike Information Criterion (<strong>AIC</strong>) values. The most impactful day of each THI was determined based on the absolute value of the greatest regression coefficient. These days were then analyzed to identify the THI breakpoint associated with a decline in egg production traits. Heat stress had a significantly negative effect on egg production traits in LHKK chickens. THI values effectively captured the impact of heat stress on these traits, with day -3 and day -1 prior egg laying exhibiting the strongest influence on egg weight at first egg and egg weight, respectively. The THI breakpoints for significant declines in egg weight at first egg were found to be higher than those for egg weight. The current findings suggest that heat stress should be considered as a significant variable in models of egg production traits in native chickens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105196"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide comparative analysis of variability and population structure between autochthonous Turkish chicken breeds and commercial hybrid lines","authors":"Eymen Demir ,&nbsp;Bahar Argun Karsli ,&nbsp;Demir Özdemir ,&nbsp;Umit Bilginer ,&nbsp;Huriye Doğru ,&nbsp;Sarp Kaya ,&nbsp;Veli Atmaca ,&nbsp;Nimet Tufan ,&nbsp;Ebru Demir ,&nbsp;Taki Karsli","doi":"10.1016/j.psj.2025.105193","DOIUrl":"10.1016/j.psj.2025.105193","url":null,"abstract":"<div><div>Next-generation sequencing (NGS) technologies have revolutionized livestock genomics by enabling rapid, high-resolution genotyping of local populations with thousands of single nucleotide polymorphisms (SNPs), offering unprecedented accuracy and cost efficiency. This study presents the first comprehensive genomic assessment of the Denizli (DNZ) and Gerze (GRZ) chicken breeds, comparing them to commercial broiler and layer hybrid lines using the double digest restriction-site associated DNA sequencing (ddRADseq) technique. A total of 94,208 bi-allelic SNPs were common between DNZ and GRZ, while 33,284 SNPs were retained among all populations after the quality filtering process. Genetic diversity parameters were higher in native Turkish chicken breeds compared to hybrid lines in which minor allele frequency (MAF) was higher than 0.3 in DNZ and GRZ while it was lower than this value in commercial hybrid lines. Notably, DNZ displayed the highest observed (0.386) and expected (0.375) heterozygosity, whereas the broiler hybrid line showed the lowest heterozygosity (0.254), suggesting inbreeding depression (FIS = 0.241). The negative inbreeding coefficient values occurring due to random mating were observed in DNZ and GRZ chicken breeds, while this value was estimated at 0.118 in the layer hybrid line. Population structure analyses such as principal component analyses (PCA), genetic distance-based neighbor-joining (NJ) tree, ADMIXTURE, and TreeMix algorithm revealed that DNZ and GRZ were genetically distinct from both each other and commercial hybrid lines. The results of this study confirm that comprehensive conservation strategies are efficient approaches to keeping genetic variability at an optimal level without inbreeding. Moreover, this study demonstrates the efficacy of ddRADseq in generating high-throughput genotypic data, providing a cost-effective framework for genomic diversity and population structure studies in indigenous chicken breeds.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105193"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cost of amino acid catabolism for energy utilization in broiler chickens","authors":"Shemil P Macelline ,&nbsp;Peter H Selle ,&nbsp;Mehdi Toghyani ,&nbsp;Sonia Y Liu","doi":"10.1016/j.psj.2025.105199","DOIUrl":"10.1016/j.psj.2025.105199","url":null,"abstract":"<div><div>This review highlights that utilization of dietary amino acids for energy metabolism in broiler chickens imposes a metabolic cost, as their primary role is to support body protein synthesis. This issue becomes more critical in reduced-crude protein (CP) diets. When amino acids are used as fuel for enterocytes or undergo catabolism in the liver, they are diverted from body protein accretion. Catabolism of amino acids for energy generates α-keto acids and ammonia. α-Keto acids can be fully oxidized to produce ATP or converted into pyruvate, ketone bodies, and intermediates of the tricarboxylic acid cycle. Meanwhile, ammonia must be detoxified through the uric acid cycle, a process that requires energy, glycine, and aspartic acid. Derivatives of α-keto acids can contribute to gluconeogenesis and <em>de novo</em> lipogenesis, leading to glucose and fatty acid synthesis, respectively. The α-keto acid derivatives are more likely to undergo <em>de novo</em> lipogenesis in broilers, as evidenced by consolidated data in this review. However, <em>de novo</em> lipogenesis is also an energy-intensive process. Therefore, enhancing the efficiency of dietary amino acid conversion to body protein requires reducing their utilization for energy metabolism. This may be achieved through dietary manipulations, as previous studies indicate that amino acid catabolism in enterocytes and the liver is influenced by starch and protein digestive dynamics, dietary amino acid compositions, and the primary feed grain used in diets. In reduced-CP broiler diets, supplementation of glutamic acid and potentially glutamine, aspartic acid, and proline could mitigate the catabolism of essential amino acids in enterocytes. Additionally, moderating starch digestion rates may reduce amino acid catabolism in enterocytes. Moreover, optimizing the balance of dietary protein-bound and non-bound amino acids could minimize amino acid catabolism in the liver. In summary, reducing the contribution of amino acids to energy metabolism in broiler chickens is particularly beneficial in reduced-CP diets, ultimately supporting more sustainable chicken meat production.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105199"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From lab to field: Innovative RPA‒CRISPR/Cas12a platform for early short-beak and dwarfism syndrome virus nucleic acids detection","authors":"Xiuqin Chen ,&nbsp;Shizhong Zhang ,&nbsp;Su Lin ,&nbsp;Meiqing Huang ,&nbsp;Shilong Chen ,&nbsp;Shao Wang","doi":"10.1016/j.psj.2025.105191","DOIUrl":"10.1016/j.psj.2025.105191","url":null,"abstract":"<div><div>Short beak and dwarfism syndrome virus (<strong>SBDSV</strong>) is the causative agent of short beak and dwarfism syndrome (<strong>SBDS</strong>), which is characterized by beak atrophy and dwarfism. SBDS has caused substantial economic losses for waterfowl husbandry industries. To address the urgent need for rapid and accurate SBDSV diagnostics, the study developed a dual-mode detection platform integrating recombinase-aided amplification (<strong>RPA</strong>) with CRISPR/Cas12a-mediated fluorescence and lateral flow strip readouts. The optimized assay achieved a detection limit of 10 copies/μL. Notably, the platform demonstrated superior specificity to distinguish SBDSV from genetically related Muscovy duck-origin goose parvovirus (<strong>GPV</strong>) and classical GPV, a distinction unachievable by qPCR. Clinical validation using 36 field samples confirmed 100% concordance with qPCR and indirect immunofluorescence assays, with no cross-reactivity against other common duck pathogens. This innovative detection system provides a robust toolkit for field-deployable SBDSV surveillance and lays a solid foundation for the development of novel diagnostic methodologies applicable to various waterfowl-related pathogens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 7","pages":"Article 105191"},"PeriodicalIF":3.8,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}