Poultry Science最新文献

{"title":"Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment","authors":"Sunisa Khongthong ,&nbsp;Pipat Piewngam ,&nbsp;Natthrit Roekngam ,&nbsp;Prapot Maliwan ,&nbsp;Sonsawan Kongpuckdee ,&nbsp;Juthatip Jeenkeawpleam ,&nbsp;Prawit Rodjan","doi":"10.1016/j.psj.2024.104518","DOIUrl":"10.1016/j.psj.2024.104518","url":null,"abstract":"<div><div>Heat stress <strong>(HS)</strong> has become a major concern for the poultry industry in many countries. HS impacts gut health by causing damaged mucosal microstructures, increased oxidative stress, weakened immunity, and heightened permeability to toxins and poultry pathogens. We investigated the potential benefits to broiler chickens subjected to HS of dietary supplementation with <em>Bacillus subtilis</em> 14823. Growth performance, gut barrier integrity, and expressions of inflammatory cytokines were analyzed. The results indicated that dietary supplementation with <em>B. subtilis</em> spores at concentrations of 1 × 10⁶ CFU/g of feed (<strong>BS6</strong> group) and 1 × 10⁷ CFU/g of feed (<strong>BS7</strong> group) improved body weight and body weight gain during d 0–42 (<em>P</em> &lt; 0.05), while the feed intake of the BS7 group was highest (<em>P</em> &lt; 0.05). Additionally, the BS6 group showed a better feed conversion ratio than the control (<strong>CON)</strong> group (<em>P</em> &lt; 0.05). The BS7 group showed the lowest serum fluorescein isothiocyanate-dextran levels (<em>P</em> &lt; 0.05), and both the BS6 and BS7 groups showed lower corticosterone levels than the CON group (<em>P</em> &lt; 0.05). Additionally, both the BS6 and BS7 groups demonstrated increased villi height and villus height/crypt depth ratio, along with decreased crypt depth in the duodenum and ileum (<em>P</em> &lt; 0.05). However, only the BS7 group exhibited greater improvements than the CON group in the jejunum at d 35. Furthermore, at d 14 and 35, mRNA expressions of occludin, claudin-1, and tight junction protein-1 in the jejunum were upregulated (<em>P</em> &lt; 0.05), and expression levels of five inflammatory cytokine genes were downregulated in the ileum (<em>P</em> &lt; 0.05). Our findings provide new insights and evidence supporting the application of <em>B. subtilis</em> 14823 for enhancing growth performance, gut barrier integrity, and modulating inflammatory cytokines in broilers.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104518"},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens","authors":"Daqing Dai,&nbsp;Xiqiong Wang,&nbsp;Kexin Wu,&nbsp;Fangren Lan,&nbsp;Jiaming Jin,&nbsp;Wenxin Zhang,&nbsp;Chaoliang Wen,&nbsp;Junying Li,&nbsp;Ning Yang,&nbsp;Congjiao Sun","doi":"10.1016/j.psj.2024.104526","DOIUrl":"10.1016/j.psj.2024.104526","url":null,"abstract":"<div><div>Proteins in egg whites play vital roles in embryonic development. Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects the structure and function of proteins. Here, we measured the phenotypes of eggs at different time points during storage and incubation and used 4D label-free quantitative proteomics technology and liquid chromatography/tandem mass spectrometry (<strong>LC-MS/MS</strong>)-technique to identify the differential proteins and N-glycosylation sites in egg whites during storage and incubation. We found that the differential N-glycoproteins in the early stage of storage were mainly related to protein structure changes, antibacterial activity, and cell proliferation, and that there were more protease inhibitors in egg whites, which decreased in the later stage of storage. Finally, eleven possible protein markers and N-glycosylation sites were identified to significantly change during storage and may exert an effect on hatchability, including the proteins involved in antibacterial activity (OVOA-N855, CLU-N154, ogchi-N82, PIGR-N290, WFDC2-N120), protein structure (LOC776816), and cell proliferation (ASAH1-N173). This study provides substantial insights into the physical and molecular compositional changes in egg whites under different storage times and revealed their potential effect on chick embryo development.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104526"},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzoic Acid potentiates intestinal IgA response in broiler chickens against Salmonella enterica Serovar Typhimurium infection","authors":"Junpeng Jia,&nbsp;Ruiling Liu,&nbsp;Rongfeng Tang,&nbsp;Jian Lin,&nbsp;Qian Yang","doi":"10.1016/j.psj.2024.104505","DOIUrl":"10.1016/j.psj.2024.104505","url":null,"abstract":"<div><div>As a feed additive, Benzoic Acid (BA) has been demonstrated to significantly enhance feed conversion efficiency, regulate gastrointestinal pH, and improve overall animal health. Young animals, highly susceptible to <em>S.</em> Typhimurium infection, suffer from high mortality rates and substantial economic losses due to this pathogen. Despite promising indications of BA's immunomodulatory potential in boosting intestinal immunity, its underlying mechanisms remain insufficiently understood. This study investigates how BA strengthens intestinal anti-infection defenses in young animals via immunomodulatory pathways, focusing on its impact on macrophage polarization and IgA-mediated immune responses. Employing in vitro cell experiments and animal models, we examined the macrophage phenotypic alterations following BA treatment. We assessed the expression of immune-related genes in the intestine through immunofluorescence staining, Western blotting, and quantitative real-time PCR. The results demonstrate that BA promotes M2 macrophage polarization by activating the mTOR/PPAR-γ/STAT3 signaling pathways. Furthermore, BA enhances the intestinal expression of the polymeric immunoglobulin receptor (<em>PIgR</em>), B-cell activating factor (<em>BAFF</em>) from the TNF family, and activation-induced cytidine deaminase (<em>AID</em>), thereby enhancing IgA production by B-cells. These results underscore the potential of BA to bolster innate immune functions in young chickens, mitigate intestinal damage caused by <em>S.</em> Typhimurium infection, and ultimately promote both animal health and food safety.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104505"},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection","authors":"Thi Hao Vu ,&nbsp;Chaeeun Kim ,&nbsp;Anh Duc Truong ,&nbsp;Jun-Mo Kim ,&nbsp;Hyun S. Lillehoj ,&nbsp;Yeong Ho Hong","doi":"10.1016/j.psj.2024.104524","DOIUrl":"10.1016/j.psj.2024.104524","url":null,"abstract":"<div><div>Avian influenza viruses (<strong>AIVs</strong>) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (<strong>lncRNAs</strong>) have emerged as crucial regulators of immune responses, yet their roles in AIV-infected chickens remain poorly understood. This study aimed to investigate the expression profiles of lncRNAs and their targets in Vietnamese Ri chickens infected with the highly pathogenic AIV (<strong>HPAIV</strong>) H5N1. Through RNA sequencing, we identified novel lncRNAs and analyzed differentially expressed (<strong>DE</strong>) transcripts at 1 and 3 days post-infection (dpi) in chicken lung tissue. Our results revealed a higher number of DE lncRNAs and mRNAs at 1 dpi and 3 dpi, respectively, compared to control, with resistant chickens exhibiting a notably stronger immune response than susceptible chickens at 3 dpi. Functional analysis implicated these lncRNAs in immune-related pathways crucial for host responses to H5N1 viral infection. Furthermore, we identified lncRNA–mRNA interactions associated with antiviral responses and immune function. Notably, several genes involved in antiviral resistance and immune responses showed higher expression in resistant chickens, confirming their stronger antiviral response. Overall, our study provides insights into the role of lncRNAs in the host's response to HPAIV H5N1 infection in chickens and highlights potential candidates for further investigation into host–pathogen interactions. These findings could drive the development of novel control strategies for AIVs, significantly enhancing poultry health and biosecurity.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104524"},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low protein diet influences mineral absorption and utilization in medium-growing yellow-feathered broilers from 1 to 30 days of age.","authors":"Xiaoran Li, Chong Wang, Shunying Li, Liyang Zhang, Xiudong Liao, Lin Lu","doi":"10.1016/j.psj.2024.104512","DOIUrl":"10.1016/j.psj.2024.104512","url":null,"abstract":"<p><p>Reduced-protein diet can save protein ingredients and reduce nitrogen (N) losses. However, the effect of low protein diet on the mineral uptake and utilization in broilers needs to be explored. The aim of this study was to investigate the effect of low-protein diet on the growth performance, N deposition, mineral contents in serum, tissues and excreta, and the activities and gene expression of related enzymes in tissues of medium-growing yellow-feathered broilers, so as to elucidate the relationship between dietary protein level and the absorption and utilization of minerals in broilers. A total of 72 1-d-old Spotted-Brown male broilers were randomly allotted to 1 of 2 treatments with 6 replicate cages of 6 birds per cage for each treatment. The dietary crude protein (CP) levels for the two treatments were 21 % (the control treatment) and 19 % (low protein treatment), respectively. The experimental period was 30 d. The results showed that no differences (P > 0.05) were detected in average daily gain, average daily feed intake and feed: gain ratio of broilers during 1 to 30 d between the two treatments. However, low protein intake increased (P < 0.05) N retention rate, serum P, Cu and Mn, and excreta Cu, Mn and Zn, and decreased (P < 0.05) liver P and excreta P. In addition, birds fed low protein diet had higher (P < 0.05) manganese superoxide dismutase, and total superoxide dismutase activities in liver, and total antioxidant capacity and malondialdehyde content in heart, and lower (P < 0.05) copper-zinc superoxide dismutase (CuZnSOD) and succinate dehydrogenase activities in liver and CuZnSOD mRNA level in heart. In conclusion, the reduction of dietary CP content from 21 % to 19 % improved N retention, the absorption of P, Cu and Mn, as well as the antioxidant ability of liver and heart, and influenced metabolic utilization of P, Cu, Zn, Fe and Mn in medium-growing yellow-feathered broilers from 1 to 30 d of age.</p>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"104512"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Simultaneous detection of GPV, H5 AIV, and GoAstV via TaqMan probe-based multiplex qPCR","authors":"Xiaoquan Wang ,&nbsp;Miao Cai ,&nbsp;Xiaolong Lu ,&nbsp;Qianqian Xu ,&nbsp;Yanhong Wang ,&nbsp;Wenhao Yang ,&nbsp;Kaituo Liu ,&nbsp;Ruyi Gao ,&nbsp;Yu Chen ,&nbsp;Jiao Hu ,&nbsp;Min Gu ,&nbsp;Shunlin Hu ,&nbsp;Xiufan Liu ,&nbsp;Xiaowen Liu","doi":"10.1016/j.psj.2024.104511","DOIUrl":"10.1016/j.psj.2024.104511","url":null,"abstract":"<div><div>The endemic status of goose parvovirus (GPV), H5 subtype avian influenza virus (AIV), and goose astrovirus (GoAstV) infections continues to devastate the poultry industry in China. Despite this, there exists a notable gap in the application of molecular diagnostic techniques. This investigation described the development of a multiplex qualitative polymerase chain reaction (qPCR) assay capable of concurrently detecting GPV, H5 AIV, and GoAstV, with no cross-reactivity observed with other avian viral pathogens. The assay exhibited a detection threshold of 10 copies/μL for both GPV and GoAstV, and 1 copy/μL for H5 AIV. The intra- and inter-assay coefficients of variation were &lt; 3.0%, signifying high repeatability within and across assay batches. Utilizing this multiplex qPCR assay, a batch of 60 clinical samples was analyzed to assess its practical utility. The detected prevalence rates for GoAstV, GPV, and H5 AIV were 35.0% (21/60), 21.7% (13/60), and 15.0% (9/60), respectively. Concurrent infections were also identified, with rates for GPV + GoAstV, GPV + H5 AIV, GoAstV + H5 AIV, and GPV + GoAstV + H5 AIV being 6.7% (4/60), 3.3% (2/60), 3.3% (2/60), and 3.3% (2/60), respectively. The developed multiplex qPCR assay exhibited a diagnostic concordance rate equivalent to that of traditional PCR techniques. This novel assay serves as a rapid, efficient, specific, and sensitive tool for the detection of prevalent goose viruses, thereby enhancing disease management strategies and epidemiological monitoring efforts.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104511"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Whole-genome sequencing revealed genomic diversity dynamics in duck conserved populations","authors":"Yu-Ze Yang ,&nbsp;Zhong-Tao Yin ,&nbsp;Xiao-Ran Lin ,&nbsp;Chun-Ying Zhao ,&nbsp;Fang-Xi Yang ,&nbsp;Fei-Fan Chen ,&nbsp;Zhuo-Cheng Hou","doi":"10.1016/j.psj.2024.104509","DOIUrl":"10.1016/j.psj.2024.104509","url":null,"abstract":"<div><div>The purpose of this study was to comprehensively analyze the genetic diversity of the Pekin duck conserved population with five generations and to evaluate the effectiveness of the current conservation strategy. In total, 277 Pekin duck conserved individuals and 40 Mallards as ancestral controls were collected. Each duck was sequenced at about 10X whole-genome coverage, while over 7.4 million single nucleotide polymorphisms (SNPs) in total were detected for genetic diversity analysis. Both the expected heterozygosity and observed heterozygosity values exceeded 0.3. The genetic differentiation (F_ST) values ranged from 0.007 to 0.039, and the Polymorphism information content (PIC) values ranged from 0.29 to 0.34. These results indicate no significant differentiation between generations, and the genetic diversity remains high. In particular, the inbreeding coefficient has been strictly controlled and has not increased rapidly during the conservation. Overall, the inbreeding coefficient of the conserved Pekin duck population was higher than that of its wild ancestors, indicating that domestication has resulted in reduced genetic diversity. This is the first report using whole genome resequencing data to systematically evaluate the genomic dynamics across several generations in ducks. The results show that the strategy of free mating and random seed retention within sire families is effective for maintaining the genetic diversity of the conserved Pekin duck population.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104509"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A PLIN1 polymorphism is associated with fat production in male emus","authors":"Yuichi Koshiishi ,&nbsp;Ryo Takahashi ,&nbsp;Michiko Murata-Okubo ,&nbsp;Yuichi Kameyama ,&nbsp;Kousaku Souma ,&nbsp;Hiroki Hirayama ,&nbsp;Kenta Wada","doi":"10.1016/j.psj.2024.104513","DOIUrl":"10.1016/j.psj.2024.104513","url":null,"abstract":"<div><div>The emu (<em>Dromaius novaehollandiae</em>) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (<strong><em>PLIN1</em></strong>) polymorphisms in the emus. We determined the partial complementary DNA (<strong>cDNA</strong>) sequence of the <em>PLIN1</em>, which is involved in lipid droplet formation. We identified four nucleotide substitution sites (c.270C&gt;T, c.321T&gt;C, c.587A&gt;T, and c.639C&gt;T) in the <em>PLIN1</em> gene of emus. Of these, c.587A&gt;T is a non-synonymous substitution that converts lysine to methionine at the 196th codon (p.K196M). Although p.K196M was predicted to affect the production traits of emus, a large deflection in genotype frequency was observed in this study; thus, we could not investigate the relationship between genotypes and phenotypes. In males, the fat yields of the CC, CT, and TT genotypes in c.270C&gt;T were 0.25 ± 0.06, 0.22 ± 0.06, and 0.21 ± 0.07 kg, respectively, while the meat yields of the CC, CT, and TT genotypes in c.270C&gt;T were 0.15 ± 0.01, 0.16 ± 0.02, and 0.16 ± 0.03 kg, respectively. These results indicate that male emus with the CC genotype had a significantly higher fat content and lower meat productivity than male emus with the other genotypes (<em>P</em> &lt; 0.05). Therefore, c.270C&gt;T in <em>PLIN1</em> affects fat and meat production in males. Our findings may contribute to the effective genetic improvement of the emus.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104513"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring poultry social dynamics using colored tags: Avian visual perception, behavioral effects, and artificial intelligence precision","authors":"Florencia B. Rossi ,&nbsp;Nicola Rossi ,&nbsp;Gabriel Orso ,&nbsp;Lucas Barberis ,&nbsp;Raul H. Marin ,&nbsp;Jackelyn M. Kembro","doi":"10.1016/j.psj.2024.104464","DOIUrl":"10.1016/j.psj.2024.104464","url":null,"abstract":"<div><div>Artificial intelligence (<strong>AI</strong>) in animal behavior and welfare research is on the rise. AI can detect behaviors and localize animals in video recordings, thus it is a valuable tool for studying social dynamics. However, maintaining the identity of individuals over time, especially in homogeneous poultry flocks, remains challenging for algorithms. We propose using differentially colored “backpack” tags (black, gray, white, orange, red, purple, and green) detectable with computer vision (eg. YOLO) from top-view video recordings of pens. These tags can also accommodate sensors, such as accelerometers. In separate experiments, we aim to: (i) evaluate avian visual perception of the different colored tags; (ii) assess the potential impact of tag colors on social behavior; and (iii) test the ability of the YOLO model to accurately distinguish between different colored tags on Japanese quail in social group settings. First, the reflectance spectra of tags and feathers were measured. An avian visual model was applied to calculate the quantum catches for each spectrum. Green and purple tags showed significant chromatic contrast to the feather. Mostly tags presented greater luminance receptor stimulation than feathers. Birds wearing white, gray, purple, and green tags pecked significantly more at their own tags than those with black (control) tags. Additionally, fewer aggressive interactions were observed in groups with orange tags compared to groups with other colors, except for red. Next, heterogeneous groups of 5 birds with different color tags were videorecorded for 1 h. The precision and accuracy of YOLO to detect each color tag were assessed, yielding values of 95.9% and 97.3%, respectively, with most errors stemming from misclassifications between black and gray tags. Lastly using the YOLO output, we estimated each bird's average social distance, locomotion speed, and the percentage of time spent moving. No behavioral differences associated with tag color were detected. In conclusion, carefully selected colored backpack tags can be identified using AI models and can also hold other sensors, making them powerful tools for behavioral and welfare studies.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 1","pages":"Article 104464"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin nuclear receptors mediate monochromatic light-induced T-lymphocyte proliferation of thymus through the AKT/GSK3β/β-catenin pathway in chick","authors":"Juanjuan Xiong ,&nbsp;Zixu Wang ,&nbsp;Yulan Dong ,&nbsp;Jing Cao ,&nbsp;Yaoxing Chen","doi":"10.1016/j.psj.2024.104507","DOIUrl":"10.1016/j.psj.2024.104507","url":null,"abstract":"<div><div>Based on previous research, it's unclear about the signaling pathway involved in the negative regulation of T-lymphocyte proliferation in thymus by monochromatic red light. Newly hatched chicks were randomly assigned divided into white (<strong>WL</strong>), red (<strong>RL</strong>), green (<strong>GL</strong>), and blue (<strong>BL</strong>) light treatments. Three days later, each light treatment group was further divided into intact, sham operation, and pinealectomy groups. The findings revealed that RL led to an increase in the expression of RORα and RORγ, while p-AKT/p-GSK3β/β-catenin/CyclinD1 expression in the thymus of chicks were decreased. Conversely, GL showed opposite results compared to RL. After pinealectomy, accompanied with the expression of RORα and RORγ increased under four light, p-AKT/ p-GSK3β/ β-catenin/ CyclinD1 expression were decreased. In vitro, exogenous melatonin increased the p-AKT/β-catenin/CyclinD1 expression in the thymic lymphocytes of chick reared under RL. The stimulative effect of melatonin was enhanced by SR3335 (RORα antagonist) or GSK298 (RORγ antagonist), while it was attenuated by SR1078 (RORα/RORγ agonist), LY-294 (PI3K antagonist) and HY-102 (AKT antagonist). These results demonstrate that RORα/RORγ negatively regulate monochromatic red light induced-T-lymphocyte proliferation in the thymus, possibly through the PI3K/AKT/p-GSK3β (Ser9) signaling pathway.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104507"},"PeriodicalIF":3.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}