Dietary DL-methionine supplementation improves growth performance of Linwu ducks by regulating redox state and intestinal mucosal morphology.

IF 4.2 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-10-01 Epub Date: 2025-07-25 DOI:10.1016/j.psj.2025.105604

Biguang Lv, Simin Peng, Cong Li, Jingmeng Zhang, Ke Jin, Xin Wang, Xinyu Lei, Xuan Cheng, Fengming Chen, Yang Liu, Guitao Jiang, De-Xing Hou, Xi He, Qiuzhong Dai, Qian Lin

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引用次数: 0

Abstract

This study was designed to investigate the effects of dietary supplementation with different levels of DL-methionine (DL-Met) on growth performance, intestinal mucosal morphology and antioxidant capacity of Linwu ducks. The experiment was conducted to classify 330 healthy 49 days of age female Linwu ducks into 5 treatment groups with 6 replicates per treatment and fed diets with DL-Met levels of 2.5 g/kg, 3.0 g/kg, 3.5 g/kg, 4.0 g/kg, and 4.5 g/kg, respectively, for 21 days. The results showed that compared to the 2.5 g/kg group, the average daily weight gain (ADG) of Linwu ducks in the 3.5 g/kg group was significantly higher (P < 0.05), while the feed-to-gain ratio (FCR) of ducks in the 4.0 g/kg and 3.5 g/kg groups was similarly significantly decreased (P < 0.05). Additionally, the duodenal, jejunal, and ileum mucosal morphology was more structurally intact in the 3.5 g/kg and 4.0 g/kg groups, and the ratio of villus height to crypt depth (V/C) was significantly improved in the duodenum and ileum (P < 0.01). On the other hand, serum triglyceride (TG) and uric acid (BUN) levels were significantly (P < 0.05) decreased in the 3.5 g/kg group. Moreover, serum malondialdehyde (MDA) levels were significantly decreased (P < 0.05) in the 3.5 g/kg and 4.0 g/kg groups, while hepatic glutathione peroxidase (GPX) activity, GPX1, and glutathione S-transferase ɑ3 (GSTα3) mRNA expression levels were significantly increased (P < 0.05). Furthermore, hepatic total antioxidant capacity (T-AOC) activity was also significantly (P < 0.05) increased in the 3.5 g/kg group. In conclusion, dietary DL-Met supplementation could improve the growth performance of Linwu ducks by improving their redox status and intestinal mucosal morphology. Under the conditions of this experiment, the appropriate level of dietary Met supplementation for 49-70 days of age Linwu ducks is between 3.5 g/kg and 4.0 g/kg.

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饲粮中添加dl -蛋氨酸通过调节氧化还原状态和肠黏膜形态改善临武鸭生长性能。

本试验旨在研究饲粮中添加不同水平dl -蛋氨酸（DL-Met）对临武鸭生长性能、肠黏膜形态和抗氧化能力的影响。试验选用健康的49日龄临武鸭母鸭330只，分为5个处理组，每个处理6个重复，分别饲喂DL-Met水平为2.5 g/kg、3.0 g/kg、3.5 g/kg、4.0 g/kg和4.5 g/kg的饲粮，试验期21 d。结果表明：与2.5 g/kg组相比，3.5 g/kg组临武鸭的平均日增重（ADG）显著提高（P < 0.05）， 4.0 g/kg和3.5 g/kg组临武鸭的料重比（FCR）同样显著降低（P < 0.05）。此外，3.5 g/kg和4.0 g/kg组十二指肠、空肠和回肠粘膜形态结构较为完整，十二指肠和回肠绒毛高度/隐窝深度（V/C）显著提高（P < 0.01）。另一方面，3.5 g/kg组血清甘油三酯（TG）和尿酸（BUN）水平显著（P < 0.05）降低。3.5 g/kg和4.0 g/kg组血清丙二醛（MDA）水平显著降低（P < 0.05），肝脏谷胱甘肽过氧化物酶（GPX）活性、GPX1和谷胱甘肽s -转移酶3 (GSTα3) mRNA表达量显著升高（P < 0.05）。3.5 g/kg组肝脏总抗氧化能力（T-AOC）活性也显著（P < 0.05）提高。由此可见，饲粮中添加DL-Met可通过改善临武鸭氧化还原状态和肠黏膜形态来改善其生长性能。本试验条件下，49 ~ 70日龄临武鸭饲粮蛋氨酸适宜添加水平为3.5 ~ 4.0 g/kg。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.