Plant Physiology and Biochemistry最新文献

{"title":"DsGGCT2-1 involved in the detoxification of Cd and Pb through the glutathione catabolism in Dianthus spiculifolius","authors":"Binbin Liu ,&nbsp;Qi Wang ,&nbsp;Jing Guan ,&nbsp;Shufang Gong ,&nbsp;Tuanyao Chai ,&nbsp;Jingang Wang ,&nbsp;Kun Qiao","doi":"10.1016/j.plaphy.2025.109976","DOIUrl":"10.1016/j.plaphy.2025.109976","url":null,"abstract":"<div><div>Toxic heavy metals seriously affect plant growth and human health. Among the heavy metals, cadmium (Cd) and lead (Pb) are serious pollutants. <em>Dianthus spiculifolius</em> has strong tolerance to, and an ability to accumulate, heavy metals. Therefore, it has potential applications as a heavy metal hyperaccumulator. Gamma glutamylcyclotransferase (GGCT) is a key enzyme in maintaining glutathione homeostasis, and it plays a role in plant growth and development and in responses to various stresses. Previously, <em>DsGGCT2-1</em> was identified as a gene showing significantly increased transcript levels in response to Cd and Pb by transcriptome analysis. In this study, DsGGCT2-1 was confirmed to increase the Cd and Pb tolerance of transgenic yeast, <em>Arabidopsis,</em> and <em>Dianthus</em>, decrease the their accumulation in <em>Dianthus</em>. Overexpression of <em>DsGGCT2-1</em> in <em>D. spiculifolius</em> plants resulted in increased GGCT activity, higher glutamate (Glu), and glutathione (GSH) content. The results suggest that more Glu is synthesized to maintain GSH homeostasis through the activity of GGCT2-1 in the glutamyl cycle, and the generated GSH is used to chelate with toxic heavy metals, and reduce the toxicity of heavy metals in the cytoplasm. These findings will be useful for devising strategies to remediate heavy metal-polluted soils, and for breeding plants that tolerate heavy metals.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109976"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the spatiotemporal strategies of plants in response to biotic and abiotic stresses:A comprehensive review","authors":"Guo Yu ,&nbsp;Jingyu Xiang ,&nbsp;Caixing Lai ,&nbsp;Xiaoming Li ,&nbsp;Geoffrey I. Sunahara ,&nbsp;Fujin Mo ,&nbsp;Xuehong Zhang ,&nbsp;Jie Liu ,&nbsp;Hua Lin ,&nbsp;Gang Liu","doi":"10.1016/j.plaphy.2025.109967","DOIUrl":"10.1016/j.plaphy.2025.109967","url":null,"abstract":"<div><div>Plant functions are governed by complex regulatory mechanisms that operate across diverse cell types in various tissues. However, the challenge of dissecting plant tissues has hindered the widespread application of single-cell technologies in plant research. Recent advancements in single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have propelled the field forward. scRNA-seq enables the examination of gene expression at the single-cell level, while ST preserves the spatial context of cellular organization. While previous reviews have discussed the breakthroughs of scRNA-seq and ST in plants, none have comprehensively addressed the use of these technologies to study plant responses to environmental stress at the cellular level. This review provides an in-depth analysis of the development, advantages, and limitations of single-cell and spatial transcriptomics, highlighting their critical role in unraveling plant strategies for coping with biotic and abiotic stresses. We also explore the challenges and future prospects of integrating scRNA-seq and ST in plant research. Understanding cell-specific responses and the complex interactions between cellular entities within the plant under stress is essential for advancing our knowledge of plant biology.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109967"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel auxin methyltransferase of the SABATH family for phenylacetic acid methylation is conserved in potato and tomato","authors":"Weijiao Wang ,&nbsp;Chi Zhang ,&nbsp;Hong Guo ,&nbsp;Feng Chen","doi":"10.1016/j.plaphy.2025.109972","DOIUrl":"10.1016/j.plaphy.2025.109972","url":null,"abstract":"<div><div>The SABATH family of methyltransferases is known for methylating a wide range of substrates, including hormones and secondary metabolites. A notable member of this family is the auxin methyltransferase IAMT which uses indole-3-acetic acid (IAA) as the substrate. This study aims to determine whether methyl phenylacetate (MePAA), the methyl ester of another auxin, phenylacetic acid (PAA), is synthesized by SABATH methyltransferases. Potato (<em>Solanum tuberosum</em> L. cv. Désirée) was chosen as the primary model because it produces MePAA exclusively in flowers. Based on the structural similarity of IAA and PAA, our initial hypothesis was that MePAA is synthesized by an IAMT-like enzyme. The potato genome contains two <em>IAMT-like</em> genes. However, their recombinant enzymes expressed in <em>Escherichia coli</em> were shown to catalyze the methylation of IAA but not PAA, thus rejecting our initial hypothesis. Among the 23 potato <em>SABATH</em> genes, two exhibited flower-specific expression. One was excluded because it had already been identified as an IAMT. <em>In vitro</em> assays of the enzyme encoded by the other gene, <em>StSABATH6</em>, confirmed its catalytic activity against PAA. Consequently, this enzyme was renamed StPAAMT. Notably, StPAAMT has an ortholog in both cultivated and wild tomatoes. The gene from tomato (<em>Solanum lycopersicum</em>), <em>SlPAAMT</em>, was verified to encode PAA methyltransferase. Further genomic and phylogenetic analyses of five <em>Solanum</em> species showed that the <em>PAAMT</em> gene was likely absent in eggplant (<em>Solanum melongena</em>), implying its origin in the common ancestor of potato and tomato. The structural analysis identified key amino acids associated with the substrate specificity of PAAMT. This work provides new insights into the evolution of auxin methyltransferases, particularly PAAMT, as members of the SABATH family.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109972"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterisation of the gibberellin-inactivating enzyme, IbCYP714A1, in sweetpotato","authors":"Meng Xing ,&nbsp;Minhong He ,&nbsp;Shulin Deng ,&nbsp;Yi Zhang ,&nbsp;Hongbo Zhu","doi":"10.1016/j.plaphy.2025.109973","DOIUrl":"10.1016/j.plaphy.2025.109973","url":null,"abstract":"<div><div>Bioactive gibberellins (GAs) are key hormones that regulate plant growth and development, playing a central role in agronomic traits such as plant height. Cytochrome <em>P450</em> genes have emerged as important regulators of GA metabolism. In rice, the <em>ELONGATED UPPERMOST INTERNODE1 (EUI1)</em> gene, and in Arabidopsis, <em>ELA1 (CYP714A1)</em> and <em>ELA2 (CYP714A2)</em>, encode P450 monooxygenases with gibberellin inactivation functions. This study aimed to identify GA-inactivating <em>P450</em> genes in sweetpotato and evaluate their functional impact on plant architecture and storage root development, which is a critical yield-related trait. In this study, we successfully identified a cytochrome <em>P450</em> gene, <em>IbCYP714A1</em>, from the sweetpotato variety Jishu26. The <em>IbCYP714A1</em> gene was highly expressed in pencil root, mature leaf, and flower tissues, and its expression was upregulated by GA treatment. We generated the <em>IbCYP714A1</em> overexpression (OE) Arabidopsis and sweetpotato, which showed similar dwarf phenotype. Additionally, the overexpression of <em>IbCYP714A</em> gene resulted in a significant inhibition of storage root expansion. Further analyses revealed that the levels of bioactive GA (GA₄ and GA₇) were significantly reduced in <em>IbCYP714A1 OE</em> sweetpotato plants. In addition, the levels of GA intermediates such as GA₉, GA₁₅, GA₂₄ and GA₅₃ were significantly reduced in overexpressing plants. This trend of changes in active gibberellin levels coincided with that of plant height changes, further confirming their close association. Meanwhile, the expression of gibberellin metabolism-related genes was decreased in <em>IbCYP714A1 OE</em> sweetpotato plants, which contributed to the reduced gibberellin levels. Taken together, our results conclude that <em>IbCYP714A1</em> play a critical role in regulating plant height and storage root development by regulating gibberellin signaling pathway in sweet potato.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"226 ","pages":"Article 109973"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous coumarin improves cell wall and plasma membrane stability and function by maintaining copper and calcium homeostasis in citrus roots under copper excess","authors":"Wei-Lin Huang ,&nbsp;Wei-Tao Huang ,&nbsp;Xu-Feng Chen ,&nbsp;Ti Wu ,&nbsp;Liang-Yuan Tong ,&nbsp;Tian-Tian Xia ,&nbsp;Bi-Sha Wu ,&nbsp;Fei Lu ,&nbsp;Ning-Wei Lai ,&nbsp;Lin-Tong Yang ,&nbsp;Li-Song Chen","doi":"10.1016/j.plaphy.2025.109949","DOIUrl":"10.1016/j.plaphy.2025.109949","url":null,"abstract":"<div><div>Most citrus trees are planted in acidic soil with high availability of copper (Cu). Little is known about the mechanisms by which coumarin (COU) reduces Cu excess in plants. ‘Xuegan’ (<em>Citrus sinensis</em>) seedlings were treated with 0.5 (Cu0.5) or 400 (Cu excess or Cu400) CuCl₂ and 0 (COU0) or 100 (COU100) μM COU for 24 weeks. COU100 alleviated Cu400-induced alterations in gene expression and metabolite profiles, cell wall (CW) materials (CWMs), CW components (CWCs), and Fourier transform infrared (FTIR) spectra of CWMs in roots; increase in Cu concentration in roots, root CWMs (RCWMs), root CWCs (RCWCs), Cu and Ca fractions in RCWMs, and Cu fraction in CW pectin; and decrease in Ca concentrations in roots, RCWMs, and RCWCs. In addition, COU100 mitigated Cu400-induced increase in electrolyte leakage and concentrations of total coumarins, total phenolics, total falvonoids, and nonstructural carbohydrates (NCs) and decrease in total free amino acid concentration in roots, as well as impairment in root system architecture (RSA) and root growth. Our results corroborated the hypothesis that the alleviation of root Cu excess by COU was caused by the combination of following several aspects: (<em>a</em>) reduced impairment to root growth and RSA; (<em>b</em>) upregulated ability to maintain CW and plasma membrane stability and function by maintaining Cu and calcium homeostasis; (<em>c</em>) elevated adaptability of primary metabolism to Cu excess; and (<em>d</em>) upregulated biosynthesis and catabolism (turnover) of secondary metabolites (SMs) and less upregulation of SMs. COU0-treated roots underwent some physiological and molecular adaptations to Cu excess.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109949"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VvNAC33 functions as a key regulator of drought tolerance in grapevine by modulating reactive oxygen species production","authors":"Na Xu ,&nbsp;Songlin Zhang ,&nbsp;Xiaoming Zhou ,&nbsp;Xiaoxuan Ma ,&nbsp;Mohabaiti Ayiguzeli ,&nbsp;Haixia Zhong ,&nbsp;Fuchun Zhang ,&nbsp;Chuan Zhang ,&nbsp;Vivek Yadav ,&nbsp;Xinyu Wu ,&nbsp;Xindi Mei","doi":"10.1016/j.plaphy.2025.109971","DOIUrl":"10.1016/j.plaphy.2025.109971","url":null,"abstract":"<div><div>Grapevine (<em>Vitis vinifera</em> L. and other <em>Vitis</em> spp.) is an important economic crop, but its yield and quality are severely affected by drought stress. NAC transcription factors, which play key roles in plant stress responses, have remained largely unexplored in grapevine drought tolerance. This study identified <em>VvNAC33</em> as a drought-responsive candidate gene through transcriptomic analysis and demonstrated its role as a positive regulator of drought tolerance. <em>VvNAC33</em> expression was significantly upregulated under drought stress. Subcellular localization and transcriptional activity analyses confirmed its nuclear localization and transcriptional activation potential. Overexpression of <em>VvNAC33</em> in <em>Arabidopsis thaliana</em> and transient overexpression in grapevine enhanced drought tolerance, whereas virus-induced gene silencing increased drought sensitivity. This enhanced tolerance was associated with the activation of the antioxidant defense system, including superoxide dismutase, peroxidase, and catalase, which promoted reactive oxygen species scavenging and alleviated oxidative damage. The enhanced expression of <em>VvCAT1</em>, <em>VvCu/ZnSOD</em>, and <em>VvPOD4</em> by <em>VvNAC33</em> highlights its crucial role in regulating antioxidant gene expression under drought stress. These findings strongly support the role of <em>VvNAC33</em> in drought tolerance and identify it as a potential molecular target for enhancing drought resistance in grapevine.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109971"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppressed OsPsbS1 expression triggers rice leaf senescence mediated by reactive oxygen species","authors":"Quanxiu Wang,&nbsp;Haolin Gao,&nbsp;Xiujie Li,&nbsp;Xinya Xu,&nbsp;Zijin Chen,&nbsp;Hualin Xu,&nbsp;Jiayi Wang,&nbsp;Si Cheng,&nbsp;Wei Zhou,&nbsp;Jinhui Zhao,&nbsp;Bo Peng","doi":"10.1016/j.plaphy.2025.109960","DOIUrl":"10.1016/j.plaphy.2025.109960","url":null,"abstract":"<div><div>Premature leaf senescence is an important factor affecting rice growth, development, and fitness. Although rice photosystem II subunit S (<em>OsPsbS1</em>) is a major gene controlling nonphotochemical quenching capacity (NPQ) in the photoprotective process, the role it plays in rice leaf senescence has not been explored yet. In this study, we use CRISPR/Cas9 technology to edit the <em>OsPsbS1</em> gene, resulting in stable homozygous lines with premature leaf senescence. The <em>Ospsbs1</em> mutant lines have pale-yellow leaves, reduced chlorophyll content, and show accelerated chloroplast degradation. Reactive oxygen species, malondialdehyde, superoxide dismutase, and peroxidase activity were significantly increased in the mutants, whereas ascorbate peroxidase and catalase activity, as well as chlorophyll content and photosynthetic rate, were markedly decreased. Furthermore, they showed increased expression of genes involved in senescence, ROS, and chlorophyll degradation. The <em>Ospsbs1</em> mutant plants were found to have severe DNA degradation and programmed cell death through TUNEL and staining, suggesting that excess ROS may cause leaf senescence. RNA sequencing analysis revealed that <em>OsPsbS1</em> is involved in the regulation of multiple biological processes, such as glutathione (GSH), starch and sucrose, and nitrogen metabolism pathways. Our results demonstrate that disruption of <em>OsPsbS1</em> can accelerate leaf senescence as a result of over-accumulation of ROS. The discovery of <em>OsPsbS1</em>'s function in controlling leaf aging in rice provides further genetic insights for understanding the molecular pathways that govern premature leaf senescence.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109960"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-application of zinc and oligosaccharides enhances zinc bioavailability, yield and nutritional quality of rice","authors":"Sajjad Ahmad,&nbsp;Xin Ouyang,&nbsp;Songpo Duan,&nbsp;Zaid Khan,&nbsp;Hong Shen","doi":"10.1016/j.plaphy.2025.109964","DOIUrl":"10.1016/j.plaphy.2025.109964","url":null,"abstract":"<div><div>Zinc (Zn) deficiency is a major abiotic factor impacting crop performance and human health. The co-application of oligosaccharides (Olg) and Zn (Olg-Zn) is an effective approach in improving Zn bioavailability, crop yield and nutritional quality. The current findings demonstrate that Olg-Zn application enhances photosynthesis, root-shoot biomass, grain yield, Zn uptake and Zn dissolution in gastric and gastrointestinal juices while reducing phytic acid and increasing Zn bioavailability. We conducted hydroponics and soil culture studies to investigate the synergy of Olg-Zn on rice growth, yield and grain quality. We found that the most effective treatments in hydroponics and soil cultures were Olg-Zn3 and Olg-ZnS2, which improved several morphological indices, such as root-shoot length and root-shoot fresh and dry weight. The findings reveal that higher photosynthesis traits and chlorophyll contents were recorded in Olg-Zn3 and Olg-ZnS2 treatments in hydroponics and soil cultures, respectively. Furthermore, compared to single Zn and Olg treatments, the Olg-Zn combination enhanced the uptake of Zn in roots, shoots and grains, resulting in higher grain yield in hydroponics (6.8 %–11.4 %) and soil culture (4.6 %–9.1 %). The application of Olg-Zn reduced phytic acid concentration by 4.7–15.3 % in hydroponics and 5.6–12.3 % in soil culture, improving Zn bioavailability by 2.2–16.6 % and 11.1–15.8 % by upregulating the expression level of Zn transporter genes, ultimately enhancing the nutritional quality of rice. Additionally, Olg-Zn improved Zn dissolution in gastric juice by 3.1–21.4 % and 3.5–19.6 %, and Zn dissolution in gastrointestinal juice was boosted by 3.7–19.7 % and 5.9–17.2 %, facilitating better Zn absorption and bioavailability in humans. However, treatments like Olg-ZnS4 and Olg-ZnS5 in soil culture slightly reduced rice yield and nutritional quality by hindering Zn bioavailability and increasing phytic acid concentration. In summary, this study highlights that an appropriate Olg-Zn combination enhances Zn uptake, leading to improved rice yield and quality, thus potentially benefitting human health.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109964"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a small secreted protein, PlSSP, that contributes to the symbiotic association of Phomopsis liquidambaris with rice under nitrogen starvation","authors":"Hao-Ming Wang ,&nbsp;Jun Zhou ,&nbsp;Chen-Yu Ma ,&nbsp;Xiao-Han Wu ,&nbsp;Yaseen Ullah ,&nbsp;Zi-Hao Zhang ,&nbsp;Yan Li ,&nbsp;Xing-Xiang Wang ,&nbsp;Chuan-Chao Dai","doi":"10.1016/j.plaphy.2025.109969","DOIUrl":"10.1016/j.plaphy.2025.109969","url":null,"abstract":"<div><div>Endophytic fungi are crucial for enhancing plant growth and stress tolerance. <em>Phomopsis liquidambaris</em> B3, a broad-spectrum endophytic fungus, significantly improves plant nitrogen uptake and growth under nitrogen-limited conditions. In this study, we identified a small secreted protein, PlSSP, which localizes to the cytoplasmic matrix of host cells and modulates plant immune responses. Using proteomic and transcriptomic approaches, we found that PlSSP upregulates key defense-related genes, including members of the PR and WRKY families, as well as genes involved in reactive oxygen species scavenging and nitrogen assimilation. Structural analysis revealed PlSSP's secondary and thermal stability features, which likely contribute to its functional interaction with host cellular components. Functional analyses demonstrated that PlSSP expression correlates with increased fungal colonization and rice biomass accumulation under nitrogen-starved conditions. These results advance our understanding of how <em>P. liquidambaris</em> promotes plant resilience and nutrient uptake, providing insights with potential applications in sustainable agriculture.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109969"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterization of prolyl oligopeptidase involved in the biosynthesis of heterophyllin B in Pseudostellaria heterophylla","authors":"Guoping Shu ,&nbsp;Wei Zheng ,&nbsp;Lanping Guo ,&nbsp;Yang Yang ,&nbsp;Changgui Yang ,&nbsp;Pengfei Li ,&nbsp;Hua he ,&nbsp;Jiao Xu ,&nbsp;Tao Zhou","doi":"10.1016/j.plaphy.2025.109970","DOIUrl":"10.1016/j.plaphy.2025.109970","url":null,"abstract":"<div><div>As a popular medicinal plant, <em>Pseudostellaria heterophylla</em> has been attached to great importance in herbal medicine. Heterophyllin B (HB), isolated from <em>P. heterophylla</em>, is a strongly pharmacologically active cyclic peptide. However, the key enzymes that catalyze the HB biosynthesis remain unidentified. In this study, we firstly screened a prolyl oligopeptidase (PhPOP1)-coding sequences through transcriptome and gene expression profile analysis, which encodes a prolyl oligopeptidase gene essential for HB biosynthesis. <em>PhPOP1</em> is highly expressed in <em>P. heterophylla</em> tuberous root phloem and xylem tissues, and localized in the endoplasmic reticulum. Heterologous transient expression assays indicated that PhPOP1 was capable of catalyzing the cyclization of the linear precursor peptide prePhHB into HB. Furthermore, overexpression of <em>PhPOP1</em> in <em>P. heterophylla</em> root cultures significantly enhanced HB production. Homologous modeling, molecular docking, and site-directed mutagenesis analysis were also conducted and it is discovered that S136, E139, A255, R369, R405, N496, D586, H604 and R664 in the binding pocket regions are vital for the activity of PhPOP1. Taken together, these findings demonstrate that <em>PhPOP1</em> plays a vital role in HB biosynthesis and therefore is a valuable candidate for increasing HB production in <em>P. heterophylla</em>. The study is the first to conduct a frontier exploration of the HB biosynthesis in <em>P. heterophylla</em> and will provide new insights into the biosynthesis of plant cyclic peptides.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109970"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}