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Cultivar-specific response of a root-associated microbiome assembly of rice to cadmium pollution 水稻根相关微生物群对镉污染的品种特异性响应
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-06-03 DOI: 10.1016/j.plaphy.2025.110128
Feng Zhang , Rui Peng , Yunhe Xie , Xionghui Ji , Saihua Liu , Huidan Jiang
{"title":"Cultivar-specific response of a root-associated microbiome assembly of rice to cadmium pollution","authors":"Feng Zhang ,&nbsp;Rui Peng ,&nbsp;Yunhe Xie ,&nbsp;Xionghui Ji ,&nbsp;Saihua Liu ,&nbsp;Huidan Jiang","doi":"10.1016/j.plaphy.2025.110128","DOIUrl":"10.1016/j.plaphy.2025.110128","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) cadmium (Cd) contamination is a serious threat to global food security and human health. However, the response of rice root-associated microbiomes to Cd pollution remains unclear. This study investigate the interactions between the root-associated microbiome and plant metals using environmental and microbial analysis methods, to reveal the potential mechanisms of the root microbiomes regulating the Cd accumulation in rice. The results showed that the grain Cd concentrations of the two low-Cd accumulation (LA) cultivars were 34–46 % lower than that of the high-Cd accumulation (HA) cultivars, whereas the iron (Fe) content in the LA roots was significantly higher than that in the HA roots. The root Fe content was significantly negatively correlated with the Cd concentration of grain (R = −0.681, <em>p</em> &lt; 0.05). 16S rRNA amplicon sequencing showed that rice planting significantly changed the diversity of the root-associated bacterial community and formed a unique core endophytic microbiome (such as Xanthobacteraceae and <em>Sphingomonas</em>) under Cd stress. LA cultivars assembled more root core microbial taxa, which promoted siderophore secretion and root Fe uptake, thereby inhibiting Cd uptake by rice. Chrome azurol S plate detection confirmed that the LA rhizosphere was enriched with 1–9-fold more siderophore-secreting microorganisms than the HA rhizosphere. This study provides new insights into the effects of root-associated microbiomes on Cd accumulation in plant and will help develop new strategies for the safe production of rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110128"},"PeriodicalIF":6.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The ZAT17-PRL1 module engages in the miRNA synthesis in Arabidopsis ZAT17-PRL1模块参与拟南芥miRNA的合成
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-06-02 DOI: 10.1016/j.plaphy.2025.110077
Qiuling Feng , Qianjin Li , Xinyu Chen , Hanyi Wu , Yixun Chen , Shaolong Jiang , Wei Yang , Shuxin Zhang
{"title":"The ZAT17-PRL1 module engages in the miRNA synthesis in Arabidopsis","authors":"Qiuling Feng ,&nbsp;Qianjin Li ,&nbsp;Xinyu Chen ,&nbsp;Hanyi Wu ,&nbsp;Yixun Chen ,&nbsp;Shaolong Jiang ,&nbsp;Wei Yang ,&nbsp;Shuxin Zhang","doi":"10.1016/j.plaphy.2025.110077","DOIUrl":"10.1016/j.plaphy.2025.110077","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) affect the expression of target mRNAs post-transcriptionally or inhibit their translation, thus controlling plant growth and stress response. Pleiotropic Regulatory Locus 1 (PRL1) plays a crucial role in the process of miRNA synthesis. However, the regulators of PRL1 downstream signaling remain largely unknown. A previous study reported that sup<em>8</em> (suppressor of <em>prl1-2</em>) can partially compensate for the development and cadmium (Cd) sensitivity of <em>prl1-2</em> mutant. The mutant gene in sup<em>8</em> encodes C2H2-type zinc finger protein ZAT17. Our research revealed that sup<em>8</em> restored the defect in miRNA expression in <em>prl1-2</em>. Through RNA-seq analysis, we discovered that ZAT17 negatively modulated the transcription of specific <em>MIR</em> genes and might affect specific biological processes, such as starch and sucrose metabolism, circadian rhythm, glycerolipid metabolism, and amino acid metabolism, via modulating the accumulation levels of particular miRNAs. Furthermore, we characterized the alternative splicing (AS) defects of <em>MIR171b</em> and <em>MIR172b</em> in <em>prl1-2</em> that were restored by <em>ZAT17</em>. Consistent with ZAT17's involvement in miRNA production, we found that ZAT17 interacts with the components of the pri-miRNA Dicer complex. These findings broaden our understanding of miRNA biosynthetic pathways and provide promising candidate targets for breeding programs aimed at enhancing plant growth and stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110077"},"PeriodicalIF":6.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significance of glutathione in the hormesis effect: a case study of the relationship between heavy metal Cd and monitoring plant Tillandsia ionantha 谷胱甘肽在激效效应中的意义:以重金属镉与监测植物蓝莲为例
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-06-02 DOI: 10.1016/j.plaphy.2025.110130
Guiling Zheng , Jingyi Zhang , Yuanyuan Liu, Shuo Han, Peng Li
{"title":"Significance of glutathione in the hormesis effect: a case study of the relationship between heavy metal Cd and monitoring plant Tillandsia ionantha","authors":"Guiling Zheng ,&nbsp;Jingyi Zhang ,&nbsp;Yuanyuan Liu,&nbsp;Shuo Han,&nbsp;Peng Li","doi":"10.1016/j.plaphy.2025.110130","DOIUrl":"10.1016/j.plaphy.2025.110130","url":null,"abstract":"<div><div>With the impact of human activities on the global environment intensifies, how different organisms adapt to environmental stressors has become a critical issue, especially to the most toxic heavy metals. Hormesis effect is an effective way to adapt to the harsh environmental changes, and glutathione (GSH) has long been considered to play an important role in the generation of hormesis, but the underlying mechanisms have not been thoroughly investigated. This experiment selected heavy metal monitoring plant <em>Tillandsia ionantha</em> as the experimental material and subjected it to cadmium (Cd) stress with different time gradients. The activity of 5 GSH-related enzymes, the expression of 5 GSH-related functional genes, and the subcellular distribution of GSH were measured. The results showed that among all 13 biomarkers, 8 exhibited hormesis effects, accounting for 62 %, significantly higher than the frequencies of other dose-response types. With prolonged Cd treatment duration, the increase in GSH content was more pronounced in leaf mitochondria, and only mitochondrial GSH content showed a significant positive correlation with Cd content, suggesting high and stable GSH levels in mitochondria appear to be critical under environmental stress conditions. Notably, the expression of the four GSH-related functional genes peaked on the second day. Factor analysis also indicated that these genes played a positive regulatory role in hormesis, suggesting that GSH-related genes may respond more rapidly to Cd stress and play a more sensitive regulatory role in the formation of hormesis. The early activation of GSH functional genes and the optimization of GSH distribution at the subcellular level are likely the core drivers of hormesis formation, which has important implications when designing strategies aiming to promote beneficial hormesis effects in organisms.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110130"},"PeriodicalIF":6.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional study of PtPTA and PtDLO1 in disease resistance of Pinellia ternata to soft rot by an efficient Agrobacterium tumefaciens-mediated transformation system 利用农杆菌介导的高效转化体系研究PtPTA和PtDLO1在半夏抗软腐病中的作用
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110097
Ming Luo , Rong Xu , Mingxing Wang , Jingyi Zhang , Binbin Liao , Xinyao Li , Yuhuan Miao , Dahui Liu
{"title":"Functional study of PtPTA and PtDLO1 in disease resistance of Pinellia ternata to soft rot by an efficient Agrobacterium tumefaciens-mediated transformation system","authors":"Ming Luo ,&nbsp;Rong Xu ,&nbsp;Mingxing Wang ,&nbsp;Jingyi Zhang ,&nbsp;Binbin Liao ,&nbsp;Xinyao Li ,&nbsp;Yuhuan Miao ,&nbsp;Dahui Liu","doi":"10.1016/j.plaphy.2025.110097","DOIUrl":"10.1016/j.plaphy.2025.110097","url":null,"abstract":"<div><div><em>Pinellia ternata</em> (Thunb.) Breit, belonging to the genus <em>Pinellia</em> in the Araceae family, is globally distributed. The dried tuber of <em>P. ternata</em> has significant medicinal value in traditional Chinese medicine, with a history of usage spanning approximately 2000 years, particularly for alleviating cough and vomiting. However, soft rot disease caused by <em>Pectobacterium carotovorum</em> poses a severe threat to <em>P</em>. <em>ternata</em> plants. This study screened 44 germplasms and identified the disease-resistant <em>Pinellia ternata</em> P-9 and disease-susceptible <em>Pinellia ternata</em> P-15. A <em>de novo</em> transcriptome analysis was conducted to explore the key genes associated with disease resistance. Among these, a mannose-binding lectin PTA gene (<em>PtPTA</em>) and a DMR6-like oxygenase 1 gene (<em>PtDLO1</em>) were identified as resistance-related genes through transient expression assays. Subsequently, a highly efficient genetic transformation system for <em>Pinellia ternata</em>, achieving a positive rate exceeding 70%, was optimized to validate the function of candidate genes. Overexpression of <em>PtPTA</em> significantly enhanced the resistance of <em>Pinellia ternata</em> to <em>Pectobacterium carotovorum</em>, while overexpression of <em>PtDLO1</em> increased susceptibility to <em>Pectobacterium carotovorum</em>. These findings highlight the pivotal roles of <em>PtPTA</em> and <em>PtDLO1</em> in conferring disease resistance in <em>Pinellia ternata</em> against soft rot, contributing to the functional analysis of key genes and resistance molecular breeding of <em>Pinellia ternata</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110097"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PbARP1 enhances salt tolerance of ‘Duli’ pear (Pyrus betulifolia Bunge) through abscisic acid signalling pathway PbARP1通过脱落酸信号通路增强‘ Duli ’梨(Pyrus betulifolia Bunge)耐盐性
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110075
Rui Liu , Ziyi Li , Huaixuan Wang , Haixia Zhang , Jianfeng Xu , Yuxing Zhang
{"title":"PbARP1 enhances salt tolerance of ‘Duli’ pear (Pyrus betulifolia Bunge) through abscisic acid signalling pathway","authors":"Rui Liu ,&nbsp;Ziyi Li ,&nbsp;Huaixuan Wang ,&nbsp;Haixia Zhang ,&nbsp;Jianfeng Xu ,&nbsp;Yuxing Zhang","doi":"10.1016/j.plaphy.2025.110075","DOIUrl":"10.1016/j.plaphy.2025.110075","url":null,"abstract":"<div><div>Pears are a widely cultivated fruit crop; however, the distribution and sustainable production are limited by salt stress. In this study, we identified 35 salt-tolerant genes by means of constructing a yeast expression library. Among the 35 genes, the transcription level of <em>PbARP1</em> in ‘Duli’ pear (<em>Pyrus betulaefolia</em> Bunge) plants is upregulated under saline stress conditions. Next, we conducted a functional analysis of the <em>PbARP1</em>, the over-expression of <em>PbARP1</em> in the transgenic pear calli lines exhibited improved tolerance to salt stresses. Conversely, the suppression of <em>PbARP1</em> resulted in enhanced sensitivity to salt stress. The transcription levels of <em>PbPYL4, PbPYL9, PbPYL8, PbSRK2I,</em> and <em>PbABI5</em> in <em>PbARP1</em> silenced pear calli and pear plants presented significant changes, which indicated these genes may be involved in the salt stress response mechanism and regulated by the <em>PbARP1</em> gene. Besides, we identified an interacting protein of PbARP1: the ABA receptor PbPYL8, highlighting the critical role of PbARP1 and the ABA signalling pathway in regulating pear response to salt stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110075"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The study on the function of exogenous NO-mediated miR166e-5p in regulating the drought resistance of alfalfa 外源no介导的miR166e-5p在苜蓿抗旱性调控中的作用研究
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110083
Xiaolin Zhu , Bochuang Wei , Baoqiang Wang , Xiaohong Wei
{"title":"The study on the function of exogenous NO-mediated miR166e-5p in regulating the drought resistance of alfalfa","authors":"Xiaolin Zhu ,&nbsp;Bochuang Wei ,&nbsp;Baoqiang Wang ,&nbsp;Xiaohong Wei","doi":"10.1016/j.plaphy.2025.110083","DOIUrl":"10.1016/j.plaphy.2025.110083","url":null,"abstract":"<div><div>The \"king of forage,\" alfalfa (<em>Medicago sativa</em> L.), is not only a high-quality forage crop but also addresses challenges arising in agricultural production and environmental governance. However, its production and application are severely impacted by drought. Physiological phenotype analysis has revealed that exogenous nitric oxide (NO) can enhance the drought tolerance of alfalfa. This study utilizes \"Sandeli\" alfalfa as the material to investigate the function of exogenous nitric oxide (NO)-mediated miR166 in regulating drought tolerance in alfalfa through the application of AmiRNA (Artificial microRNA) technology and STTM (Short Tandem Target Mimic) technology. The regulation of drought resistance in miR166-transformed alfalfa by exogenous nitric oxide (NO) indicates that silencing miR166e-5p leads to the serration of alfalfa leaf margins and delayed wilting in detached leaves. The content of proline, an osmoregulatory substance, increases to 1.58 times that of the wild type. The activities of antioxidant enzymes increase to 1.39 to 1.59 times those of the wild type, while the contents of H<sub>2</sub>O<sub>2</sub> (Hydrogen Peroxide) and MDA (Malondialdehyde) decrease to 61 % and 72 % of the wild type, respectively. Additionally, the expression of target genes and NO synthase genes is upregulated, with the NR2 gene showing a 2.27-fold upregulation. Overexpression of miR166e-5p exacerbates the damage to the antioxidant system and reduces osmoregulatory substances. In summary, silencing miR166e-5p enhances the drought tolerance of alfalfa. The application of exogenous NO causes the physiological indicators and gene expression levels in the overexpressing lines to trend towards those of the wild type, effectively reducing the drought sensitivity caused by miR166e-5p overexpression. This study screens out miR166-5p related to drought tolerance, laying a theoretical foundation for the rapid breeding of drought-tolerant alfalfa.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110083"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of non-destructively measured wheat traits sensitive to nitrogen application and closely related to wheat yields 对施氮敏感且与产量密切相关的小麦无损检测性状的选择
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110088
Feng Li , Yang Yang , Xiaoyu Ni , Ye Yang , Sinan Li , Jiading Yuan , Qing Ma , Na Li
{"title":"Selection of non-destructively measured wheat traits sensitive to nitrogen application and closely related to wheat yields","authors":"Feng Li ,&nbsp;Yang Yang ,&nbsp;Xiaoyu Ni ,&nbsp;Ye Yang ,&nbsp;Sinan Li ,&nbsp;Jiading Yuan ,&nbsp;Qing Ma ,&nbsp;Na Li","doi":"10.1016/j.plaphy.2025.110088","DOIUrl":"10.1016/j.plaphy.2025.110088","url":null,"abstract":"<div><div>Rapid and non-destructive detection of crop growth traits can guide nitrogen (N) diagnosis and management. However, there is a lack of comprehensive studies on multiple non-destructively measured crop traits under different N inputs. The study aimed to investigate the responses of 36 non-destructively measured wheat traits to N application rates, and to assess which traits are sensitive to N application and closely related to wheat yields. The 36 traits included plant shape traits, physiological traits, physical traits, and leaf color traits. Field experiment included 24 treatments, i.e., the combination of eight N application rates (0, 50, 100, 150, 200, 250, 300, and 350 kg N ha<sup>−1</sup>) and three farmlands (farmland converted from wasteland for 2, 5, and 8 years, denoted as Farmland<sub>2yr</sub>, Farmland<sub>5yr</sub>, and Farmland<sub>8yr</sub>). Results showed that the N application rate for the greatest grain yield was 350.0 (Farmland<sub>2yr</sub>), 286.7 (Farmland<sub>5yr</sub>), and 217.6 kg N ha<sup>−1</sup> (Farmland<sub>8yr</sub>). Nitrogen application tended to increase most plant shape, physiological, and physical traits; while it tended to reduce most leaf color traits. Among the 36 traits studied, 19 traits were sensitive to N application and closely related to wheat yields. The 19 traits were plant height, stem diameter, mean leaf length, mean leaf width, mean leaf area, mean leaf volume, single stem leaf area, leaf area index, leaf SPAD, leaf quantum yield, leaf N content, leaf surface moisture, leaf surface temperature, canopy radiation transmittance, leaf NBI, RDBI, GDBI, BDBrI, and RDLI. These traits can be considered for the establishment of rapid N diagnosis systems in wheat production. The findings are expected to deepen our understanding of the responses of multiple non-destructively measured plant traits to N levels, and provide essential information for the establishment of rapid N diagnosis systems and grain yield prediction models.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110088"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved water-use strategies in oilseed rape transformed with Rhizobium rhizogenes under soil drought 土壤干旱条件下根瘤菌转化油菜改良水分利用策略
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110117
Xuefei Chen , Bruno Trevenzoli Favero , Xizi Wang , Ellen de Keyser , Fulai Liu , Henrik Lütken
{"title":"Improved water-use strategies in oilseed rape transformed with Rhizobium rhizogenes under soil drought","authors":"Xuefei Chen ,&nbsp;Bruno Trevenzoli Favero ,&nbsp;Xizi Wang ,&nbsp;Ellen de Keyser ,&nbsp;Fulai Liu ,&nbsp;Henrik Lütken","doi":"10.1016/j.plaphy.2025.110117","DOIUrl":"10.1016/j.plaphy.2025.110117","url":null,"abstract":"<div><div>Root-inducing (Ri) lines are plants that have undergone transformation with <em>Rhizobium rhizogenes</em> in which the transfer (T)-DNA of the bacterium has been incorporated into their genomes. Two Ri lines of <em>Brassica napus</em> (A11 and B3), developed in our lab, demonstrated improved tolerance to osmotic stress compared to the wild-type (WT) plants grown in hydroponics. However, these responses have not been confirmed for plants grown under soil drought. In the current study, A11 and B3 exhibited better maintenance of hydraulic integrity under conditions of severe soil drought, as exemplified by higher leaf water potential and delayed decline of daily plant water consumption in response to drought. In respect to the higher water maintenance, both Ri lines exhibited lower stomatal conductance (<em>g</em><sub>s</sub>) and transpiration rate (<em>T</em><sub>r</sub>) (0.4 times lower than WT) under drought, which could be attributed to their significantly higher concentrations of leaf abscisic acid (ABA). Therefore, the improved water-use strategies in the Ri lines under severe soil drought could be attributed to ABA-mediated fine-tuned control of <em>g</em><sub>s</sub> and thus <em>T</em><sub>r</sub> compared to WT. We furthermore analyzed the T-DNA insertion events in the two Ri lines and found that a high copy number of T<sub>L</sub>-DNA genes (<em>rolA</em>, <em>rolB</em>, <em>rolC</em>, <em>rolD,</em> and ORF13) and complete T-DNA insertion (both T<sub>L</sub>-DNA and T<sub>R</sub>-DNA) seemed to be correlated with a more severe Ri phenotype (e.g., dwarfing). Finally, the potential association of morphological modifications after T-DNA insertion with drought resilience in Ri oilseed rape was discussed. These findings provide evidence that <em>R. rhizogenes</em>-mediated transformation can be beneficial for developing pre-breeding oilseed rape lines coping better with drought stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110117"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CiBBX19 negatively regulates the flowering time of Chrysanthemum indicum by recruiting CiBBX5 to inhibit the expression of CiFTL3 CiBBX19通过募集CiBBX5抑制CiFTL3的表达,负向调控菊花开花时间
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110115
Chenlu Yang , Shuaiwei Zhang , Qingjun Zou, Chang Liu, Peng Chen, Tao Wang, Qiaosheng Guo
{"title":"CiBBX19 negatively regulates the flowering time of Chrysanthemum indicum by recruiting CiBBX5 to inhibit the expression of CiFTL3","authors":"Chenlu Yang ,&nbsp;Shuaiwei Zhang ,&nbsp;Qingjun Zou,&nbsp;Chang Liu,&nbsp;Peng Chen,&nbsp;Tao Wang,&nbsp;Qiaosheng Guo","doi":"10.1016/j.plaphy.2025.110115","DOIUrl":"10.1016/j.plaphy.2025.110115","url":null,"abstract":"<div><div>Flowering is a critical stage in plant growth and reproduction. <em>Chrysanthemum indicum</em>, a species with both ornamental and medicinal value, has its flowering time directly affecting the quality of inflorescences. This study elucidates the molecular mechanism by which the BBX transcription factor family members CiBBX19 and CiBBX5 coordinately regulate the flowering time of <em>Chrysanthemum indicum</em>. Phylogenetic analysis reveals that CiBBX19 is the ortholog of AtBBX19, with its expression significantly downregulated during the vegetative-to-reproductive transition in leaves. Functional validation demonstrates that overexpression of CiBBX19 delays flowering, while its silencing accelerates this developmental transition, confirming its biological role in maintaining vegetative growth. Through qPCR time-course analysis and genetic transformation experiments, we identified the short-day responsive florigen gene <em>CiFTL3</em> in <em>Chrysanthemum indicum</em>. The expression of <em>CiFTL3</em> surges during the flower bud differentiation stage, and its overexpression advances the flowering time. The expression of <em>CiFTL3</em> significantly decreases in CiBBX19-overexpressing lines, but yeast one-hybrid and LUC assays confirm that CiBBX19 does not directly bind to the <em>CiFTL3</em> promoter. Protein interaction analysis reveals that CiBBX19 forms a heterodimer with CiBBX5. Although CiBBX5 exhibits stable expression during the developmental transition period, it specifically binds to the G-box cis-element (TACGTG) of the <em>CiFTL3</em> promoter, thereby suppressing the expression of <em>CiFTL3</em> and delaying the flowering in <em>Chrysanthemum indicum</em>. Through dual-luciferase reporter assays, we established that CiBBX19-CiBBX5 heterodimerization synergistically enhances transcriptional repression of <em>CiFTL3</em>, exceeding the inhibitory effect mediated by CiBBX5 alone. Our experiment showed that CiBBX19 can interact with CiBBX5 to indirectly inhibit the transcription of <em>CiFTL3</em>. This synergistic regulatory mechanism provides new insights into unraveling the dynamic balance between vegetative growth and reproductive development in Asteraceae plants and lays a molecular foundation for precise regulation of <em>Chrysanthemum indicum</em> flowering time.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110115"},"PeriodicalIF":6.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide identification of the PYL gene family and functional validation of SiPYL3 in foxtail millet (Setaria italica) 谷子PYL基因家族的全基因组鉴定及SiPYL3基因的功能验证
IF 6.1 2区 生物学
Plant Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1016/j.plaphy.2025.110118
Xiaoqian Ma , Jiafan Qin , Bing Guo , Meng Jia , Guozhang Li , Longjie Li , Na Li , Shutao Dai , Cancan Zhu , Zanping Han , Xiaoyan Li , Zhongling Liu , Chentong Yu , Na Qin , Junxia Li
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