Plant Physiology and Biochemistry最新文献_第8页

Functional characterization of TrGSTF15, a glutathione S-transferase gene family member, on the transport and accumulation of anthocyanins and proanthocyanidins in Trifolium repens 谷胱甘肽 S-转移酶基因家族成员 TrGSTF15 对三叶草花青素和原花青素的运输和积累的功能表征

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-15 DOI: 10.1016/j.plaphy.2024.109038

{"title":"Functional characterization of TrGSTF15, a glutathione S-transferase gene family member, on the transport and accumulation of anthocyanins and proanthocyanidins in Trifolium repens","authors":"","doi":"10.1016/j.plaphy.2024.109038","DOIUrl":"10.1016/j.plaphy.2024.109038","url":null,"abstract":"<div>Anthocyanins and proanthocyanidins (PAs) are important secondary metabolites in plants, high contents of which are an important goal for quality breeding of white clover (Trifolium repens). However, the involvement of glutathione S-transferase (GST) in the transport of anthocyanins and PAs remains unexplored in white clover. This study identified 153 different TrGSTs in white clover. At the transcriptional level, compared to other TrGSTFs, TrGSTF10 and TrGSTF15 are highly expressed in the ‘Purple’ white clover, and they may work with the anthocyanin biosynthesis structural genes CHS and CHI to contribute to pigment buildup in white clover. Subcellular localization confirmed that TrGSTF10 and TrGSTF15 are located in the cytoplasm. Additionally, molecular docking experiments showed that TrGSTF10 and TrGSTF15 have similar binding affinity with two flavonoid monomers. Overexpression of TrGSTF15 complemented the deficiency of anthocyanin coloring and PA accumulation in the Arabidopsis tt19 mutant. The initial findings of this research indicate that TrGSTF15 encodes an important transporter of anthocyanin and PA in white clover, thus providing a new perspective for the further exploration of related transport and regulatory mechanisms.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid: An emerging player in plant-virus interactions 脱落酸：植物与病毒相互作用中的新角色

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-14 DOI: 10.1016/j.plaphy.2024.109046

{"title":"Abscisic acid: An emerging player in plant-virus interactions","authors":"","doi":"10.1016/j.plaphy.2024.109046","DOIUrl":"10.1016/j.plaphy.2024.109046","url":null,"abstract":"<div>In the evolutionary arm race between plants and viral pathogens, the plant hormone abscisic acid (ABA) has surfaced as a crucial player. This review accumulates substantial evidence that portrays ABA as a crucial regulatory hub, coordinating the complex network of plant antiviral immunity. It is capable of synchronizing resistance pathways, yet it can also be exploited as a susceptibility factor by viral effectors. ABA fortifies multi-layered defenses on one hand, by activating RNA silencing mechanisms that precisely degrade viral genomes, strengthening plasmodesmal gateways with callose barriers, and priming the transcriptional programs of resistance genes. On the other hand, ABA can augment susceptibility by counteracting other defense hormones, dampening oxidative bursts, and inhibiting antiviral defence proteins. Interestingly, a variety of viruses have independently evolved strategies to manipulate ABA signalling pathways. This fascinating paradigm of hormonal conflicts unveils ABA as an important regulatory handle that determines infection trajectories. Future studies should carefully explore the multifaceted impacts of ABA modulation on plant immunity and susceptibility to diverse pathogens before considering practical applications in viral resistance strategies.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progresses in biosynthesis pathway, regulation mechanism and potential application of 2-acetyl-1-pyrroline in fragrant rice 香稻中 2-乙酰基-1-吡咯啉的生物合成途径、调控机制及潜在应用研究进展

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-14 DOI: 10.1016/j.plaphy.2024.109047

引用次数: 0

Enhancing garlic propagation through functional biopolymer-based propagules coatings: A bio-nanotechnological strategy 通过基于功能性生物聚合物的繁殖体涂层提高大蒜繁殖能力：生物纳米技术战略

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-14 DOI: 10.1016/j.plaphy.2024.109049

{"title":"Enhancing garlic propagation through functional biopolymer-based propagules coatings: A bio-nanotechnological strategy","authors":"","doi":"10.1016/j.plaphy.2024.109049","DOIUrl":"10.1016/j.plaphy.2024.109049","url":null,"abstract":"<div>Integrating agricultural, chemical, and technological knowledge is crucial for developing bio-nanotechnologies to improve agricultural production. This study explores the innovative use of biopolymeric coatings, based on sodium alginate and sodium alginate + Laponite® (nanoclay), containing biostimulants (tryptophol and thymol) or not, on garlic cloves. These coatings were analyzed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR-ATR), and scanning electron microscopy (SEM). Greenhouse bioassays showed improvements in garlic shoot plant biomass with both treatments: sodium alginate biopolymer and sodium alginate biopolymer plus Laponite®. In the field experiment, garlic plants treated with sodium alginate, in combination with conventional pesticide treatments, resulted in better quality garlic bulbs, where larger garlics were harvested in this treatment, reducing commercial losses. In tropical garlic crops, obtaining plants with greater initial vigor is essential. Our results highlight the potential of these bio-nanotechnological strategies to enhance garlic propagation, ensuring environmental protection and food security.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light green leaf sectors of variegated Dracaena fragrans plants show similar rates of oxygenic photosynthesis tо that of normal, dark green leaf sectors 变色龙脑香科植物的浅绿色叶片与正常深绿色叶片的含氧光合作用速率相似

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-13 DOI: 10.1016/j.plaphy.2024.109040

{"title":"Light green leaf sectors of variegated Dracaena fragrans plants show similar rates of oxygenic photosynthesis tо that of normal, dark green leaf sectors","authors":"","doi":"10.1016/j.plaphy.2024.109040","DOIUrl":"10.1016/j.plaphy.2024.109040","url":null,"abstract":"<div>Adaptation and functional significance of chlorophyll deficit in the light green leaf sectors of variegated plants are little known. Efficiency of photosystem II for dark and light adapted states (Fv/Fm and ΔF/Fm’) and fluorescence decrease rates (Rfd) of light green leaf sectors of Dracaena fragrans L. were studied by methods of PAM-fluorometry and video registration. In addition, white light reflectance and transmittance of these leaf sectors were measured using an integrating sphere. Absorption was calculated from reflectance and transmittance. Net CO2 assimilation rates (PN) were measured using a flow chamber and photolytic O2 evolution rates (PAYO2) were studied by a novel method of Fourier photoacoustics which is insensitive to respiration, photorespiration and other processes of O2 uptake. All the photosynthetic parameters (Fv/Fm, ΔF/Fm’, PN and PAYO2) were found to be very close between light green and normal green leaf sectors, whereas chlorophyll content and light absorption were 7.5-fold and 1.47-fold different respectively. Contradiction between low chlorophyll absorption and high (as in normal green sectors) rate of oxygenic photosynthesis in light-green sectors was proposed to be a consequence of different contribution of cyclic electron transport around PSII (CET-PSII) and/or around PSI (CET-PSI) in the total photosynthesis occurring in these sectors. Particularly, it cannot be excluded, that some part of CET activity occurring in normal green leaf sectors may be lost in the light green sectors retaining the same linear (non-cyclic) electron transport (LET) activity as in normal green sectors.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristic of epicotyl dormancy and its hormonal regulation in Chinese cork oak (Quercus variabilis) 中国栓皮栎（Quercus variabilis）外胚轴休眠的特征及其激素调控

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-13 DOI: 10.1016/j.plaphy.2024.109041

{"title":"Characteristic of epicotyl dormancy and its hormonal regulation in Chinese cork oak (Quercus variabilis)","authors":"","doi":"10.1016/j.plaphy.2024.109041","DOIUrl":"10.1016/j.plaphy.2024.109041","url":null,"abstract":"<div>Emergence heterogeneity caused by epicotyl dormancy contributes to variations in seedling quality during large-scale breeding. However, the mechanism of epicotyl dormancy release remains obscure. We first categorized the emergence stages of Chinese cork oak (Quercus variabilis) using the BBCH-scale. Subsequently, we identified the key stage of the epicotyl dormancy process. Our findings indicated that cold stratification significantly released epicotyl dormancy by increasing the levels of gibberellic acid 3 (GA3) and GA4. Genes associated with GA biosynthesis and signaling also exhibited altered expression patterns. Inhibition of GA biosynthesis by paclobutrazol (PAC) treatment severely inhibited emergence, with no effect on seed germination. Different concentrations (50 μM, 100 μM, and 200 μM) of GA3 and GA4+7 treatments of germinated seeds demonstrated that both can promote the emergence, with GA4 exhibiting a more pronounced effect. In conclusion, this study provides valuable insights into the characterization of epicotyl dormancy in Chinese cork oak and highlights the critical role of GA biosynthesis in seedling emergence. These findings serve as a basis for further investigations on epicotyl dormancy and advancing large-scale breeding techniques.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142049812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring phytoremediation potential of willow NJU513 for cadmium-contaminated soil with and without epibrassinolide treatment 探索柳树 NJU513 在经过或未经过表紫草内酯处理的情况下对镉污染土壤进行植物修复的潜力

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-13 DOI: 10.1016/j.plaphy.2024.109044

{"title":"Exploring phytoremediation potential of willow NJU513 for cadmium-contaminated soil with and without epibrassinolide treatment","authors":"","doi":"10.1016/j.plaphy.2024.109044","DOIUrl":"10.1016/j.plaphy.2024.109044","url":null,"abstract":"<div>There has been a growing concern over soil cadmium (Cd) pollution, underscoring the importance of finding effective remediation strategies. Willow trees have emerged as promising candidates for phytoremediation of Cd-contaminated soils. Nevertheless, the specific potential of a novel willow genotype, NJU513, in remediating Cd-polluted soil remains unexplored. Hence, the primary objectives of this study were twofold: firstly, to ascertain the suitability of the willow genotype NJU513 for remediating Cd-contaminated soil; and secondly, to elevate its remediation efficciency with the application of epibrassinolide (Brs). In the pot-culture experiment without Brs, its leaf and stem Cd concentrations were 203 mg kg−1 and 65.1 mg kg−1, with a bioaccumulation factor (BCF) of 20.8 and 6.68, respectively. In the pot-culture experiment with Brs, the corresponding Cd concentrations were 226 mg kg−1 and 59.2 mg kg−1, with a BCF of 23.1 and 6.06, respectively. In addition, the extracted Cd contents were higher in the Brs treatments (1.11–1.37 mg plant−1) than in the no-Brs treatments (0.78–0.96 mg plant−1) because Brs increased the plant biomass and leaf BCF. The mechanism underlying the Cd accumulation of NJU513 leaves with and without Brs was revealed by a transcriptome analysis. The expression levels of genes related to metal ion binding, channel activity, and transporters in leaves were up-regulated, which contributed to the high Cd accumulation and stress tolerance. Analyses of soil metabolites and bacteria in the presence and absence of Brs spraying on willow leaves indicated that soil organic compounds with carboxyl and amino groups may induce Cd activation and passivation, respectively. This study provides valuable insights for developing woody plant varieties that can be used for remediating Cd-contaminated soil.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142039636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of ascorbate redox turnover in iron toxicity tolerance 抗坏血酸氧化还原在铁毒性耐受性中的作用

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-13 DOI: 10.1016/j.plaphy.2024.109045

{"title":"The role of ascorbate redox turnover in iron toxicity tolerance","authors":"","doi":"10.1016/j.plaphy.2024.109045","DOIUrl":"10.1016/j.plaphy.2024.109045","url":null,"abstract":"<div>Iron (Fe) toxicity is a major abiotic stress in lowland rice production. Breeding tolerant varieties has proven challenging due to the complex genetic architecture of Fe toxicity tolerance and the strong genotype-by-environment interactions. Additionally, conventional methods for phenotyping visible stress symptoms are often inaccurate, inconsistent, and lack reproducibility.In our previous work, we identified that ascorbate redox regulation, mediated by the activities of dehydroascorbate reductase (DHAR) and ascorbate oxidase (AO), contributed to high tolerance in an indica rice genotype across various environments. To explore whether this mechanism is common among other rice genotypes, we selected ten genotypes with contrasting stress symptoms under Fe-toxic conditions to examine the roles of DHAR and AO in regulating Fe toxicity tolerance. Additionally, we aimed to develop objective and accurate image-based phenotyping methods to replace the traditional leaf bronzing scoring method.Among the ten genotypes we tested, we found significant positive correlations between DHAR activity and stress symptoms in plants grown under both Fe toxicity and control conditions, suggesting a general link between ascorbate redox regulation and Fe toxicity tolerance. Using RGB signals from leaf images of plants exposed to 1000 mg/L Fe2+, we evaluated 36 different color indices to quantify stress symptoms. We identified the normalized green‒red difference index as most significant in quantifying stress symptoms under Fe toxicity conditions.Our findings suggest that DHAR activity could be potentially employed as a biomarker in the screening of rice germplasms and breeding tolerant cultivars to Fe toxicity.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlCIPK9 regulates pollen tube elongation in tomato plants via a K+-independent mechanism SlCIPK9 通过与 K+ 无关的机制调节番茄花粉管的伸长

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-12 DOI: 10.1016/j.plaphy.2024.109039

{"title":"SlCIPK9 regulates pollen tube elongation in tomato plants via a K+-independent mechanism","authors":"","doi":"10.1016/j.plaphy.2024.109039","DOIUrl":"10.1016/j.plaphy.2024.109039","url":null,"abstract":"<div>Potassium (K+) is an essential macronutrient which contributes to osmotic- and turgor-related processes in plants. Calcineurin-B like Interacting Protein Kinases (CIPKs) play crucial roles in plants under low-K+ supply since they activate root K+ uptake transport systems such as AKT1 and AtHAK5. In Arabidopsis, AtCIPK9 is important for low-K+ tolerance since atcipk9 plants exhibited poor growth and leaf chlorosis when K+ was scarce. Part of these phenotypes could be ascribed to the activation of AtHAK5 by AtCIPK9. It has been reported that important differences exist between Arabidopsis and other plant species such as tomato with respect to the regulation of K+ uptake systems. Thus, our aim was to evaluate the contribution of SlCIPK9, the homologous protein of AtCIPK9 in tomato, to K+ nutrition. Unexpectedly, phenotyping experiments carried out with slcipk9 loss-of-function mutants revealed that SlCIPK9 did not play a clear role in tomato K+ homeostasis. By contrast, it was found that SlCIPK9 contributed to pollen tube elongation, but not to pollen germination, via a K+-independent mechanism. Therefore, our results highlight the remarkable differences that exist in Ca2+ signaling pathways between plant species and encourage the realization of more comparative studies as the one presented here.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007071/pdfft?md5=17f970ccbd99b7a4336359a3a580b4c6&pid=1-s2.0-S0981942824007071-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JrPPO1/2 play distinct roles in regulating walnut fruit browning by different spatiotemporal expression and enzymatic characteristics JrPPO1/2在调控核桃果实褐变的过程中发挥着不同的作用，具有不同的时空表达和酶学特征。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-08-12 DOI: 10.1016/j.plaphy.2024.109018

{"title":"JrPPO1/2 play distinct roles in regulating walnut fruit browning by different spatiotemporal expression and enzymatic characteristics","authors":"","doi":"10.1016/j.plaphy.2024.109018","DOIUrl":"10.1016/j.plaphy.2024.109018","url":null,"abstract":"<div>Polyphenol oxidase (PPO) activity drives walnut fruit browning, but the roles of its only two-family genes, JrPPO1 and JrPPO2, remain unclear. This study explores the spatiotemporal expression and enzymatic characteristics of JrPPO1 and JrPPO2 in walnut. Treatment with the PPO activator CuSO4 and H2O2 accelerated fruit browning and up-regulated JrPPO1/2 expression, whereas treatment with the PPO inhibitor ascorbic acid delayed browning, down-regulating JrPPO1 and up-regulating JrPPO2 expression. Compared to mJrPPO1, mJrPPO2 can exhibited better enzyme activity at higher temperatures (47 °C) and in more acidic environments (pH 4.25). mJrPPO2 exhibited a higher substrate specificity over mJrPPO1, and the preferred substrates are catechol, chlorogenic acid, and epicatechin. Additionally, mJrPPO2 adapted better to low concentration of oxygen (as low as 1.0% O2) and slightly elevated CO2 levels compared to mJrPPO1. Subcellular localization and spatiotemporal expression patterns showed that JrPPO1 is only expressed in green tissues and located in chloroplasts, while JrPPO2 is also located in chloroplasts, partly associated with membranes, and is expressed in both green and non-green tissues. Silencing JrPPO1/2 with virus-induced gene silencing (VIGS) reduced fruit browning, maintained higher total phenols, and decreased MDA production. Notably, silencing JrPPO1 had a greater impact on browning than JrPPO2, indicating JrPPO1's greater contribution to PPO activity and fruit browning in walnut fruits. Consequently, JrPPO1 can be effectively regulated both at the molecular level and by manipulating environmental conditions, to achieve the objective of controlling fruit browning.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0