Plant Physiology and Biochemistry最新文献_第8页

Genome-wide identification, phylogeny, evolutionary expansion, and expression analyses of ABC gene family in Castanea mollissima under temperature stress. 温度胁迫下板栗ABC基因家族的全基因组鉴定、系统发育、进化扩展及表达分析

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-24 DOI: 10.1016/j.plaphy.2024.109450

Liyang Yu, Yujuan Tian, Xiangyu Wang, Fei Cao, Haifen Wang, Ruimin Huang, Chunlei Guo, Haie Zhang, Jingzheng Zhang

{"title":"Genome-wide identification, phylogeny, evolutionary expansion, and expression analyses of ABC gene family in Castanea mollissima under temperature stress.","authors":"Liyang Yu, Yujuan Tian, Xiangyu Wang, Fei Cao, Haifen Wang, Ruimin Huang, Chunlei Guo, Haie Zhang, Jingzheng Zhang","doi":"10.1016/j.plaphy.2024.109450","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109450","url":null,"abstract":"The ATP-binding cassette (ABC) gene family comprises some of the most critical transporter proteins in plants, playing vital roles in maintaining cellular homeostasis and adapting to environmental changes. While ABC transporters have been extensively characterized in various plant species, their profile in C. mollissima remains less understood. In this study, 164 ABC genes were identified and characterized within the C. mollissima genome, and subsequently classified into eight subfamilies. Collinear analysis suggested that dispersed duplication was the primary mechanism driving the expansion of the CmABC gene family. The study also examined morphological and physiological changes in C. mollissima under temperature stress, highlighting significant decreases in photosynthetic indicators and SOD enzyme activity, while other indicators varied. Transcriptome analysis revealed distinct expression patterns of various CmABC genes under temperature stress, identifying CmABCG29c and CmABCB11e as key candidates for responding to temperature stress. This was based on their expression patterns, correlation with physiological indicators, and WGCNA analysis. The expression levels of CmABC genes measured in RT-qPCR experiments were consistent with those observed in RNA-seq analysis. This research provides a theoretical foundation for understanding the physiological and gene expression responses of C. mollissima to temperature stress.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109450"},"PeriodicalIF":6.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mowing facilitated Pb accumulation in bermudagrass by mediating root radial transport. 刈割通过调节根径向运输促进了百慕大草Pb的积累。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-24 DOI: 10.1016/j.plaphy.2024.109443

Xinyi Cai, Yongjun Yue, Yike Wang, Liyin Zhang, Mingyan Jiang, Xiaofang Yu, Lingxia Sun, Zhuo Huang, Baimeng Guo, Donglin Zhang, Xi Li

{"title":"Mowing facilitated Pb accumulation in bermudagrass by mediating root radial transport.","authors":"Xinyi Cai, Yongjun Yue, Yike Wang, Liyin Zhang, Mingyan Jiang, Xiaofang Yu, Lingxia Sun, Zhuo Huang, Baimeng Guo, Donglin Zhang, Xi Li","doi":"10.1016/j.plaphy.2024.109443","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109443","url":null,"abstract":"Moderate mowing of the shoot is an effective strategy for improving Pb-contaminated soil remediation using bermudagrass. However, the mechanisms by which mowing facilitates Pb uptake and accumulation remain insufficiently understood. Root radial transport is critical in efficient heavy metal uptake and translocation in plants and is influenced by root physiological-biochemical characteristics. Herein, radial transport in roots and its effect on root-shoot Pb transport in bermudagrass under mowing were explored. Results revealed that mowing decreased Pb in apoplasts and increased Pb in symplasts, altering Pb radial transport pathways in roots. In the apoplastic pathway, mowing pretreatment intensified the inhibitory effects of a transpiration inhibitor on Pb uptake, resulting in a reduced contribution of the apoplastic pathway. Mowing induced lateral root endodermis thickening, early suberin lamellar development and increased suberin deposition, effectively preventing Pb from entering the stele through the apoplastic pathway. Conversely, in the symplastic pathway, mowing pretreatment alleviated the inhibitory effects of a metabolic inhibitor and ion channel inhibitor on Pb uptake and significantly increased net Pb2+influx in lateral root tips, thereby promoting the symplastic pathway. Furthermore, mowing upregulated the relative expression of CdNramp5 and CdHMA2 in roots, increasing Pb translocation to the shoot via the symplastic pathway. Overall, our study provided novel evidence mowing primarily improved Pb uptake and root-to-shoot transport by increasing the efficiency of the symplastic pathway. These findings provide a theoretical foundation for the use of mowing to improve the efficacy of bermudagrass in the remediation of Pb-contaminated soils.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109443"},"PeriodicalIF":6.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The glutathione S-transferase BnGSTU12 enhances the resistance of Brassica napus to Sclerotinia sclerotiorum through reactive oxygen species homeostasis and jasmonic acid signaling. 谷胱甘肽s -转移酶BnGSTU12通过活性氧稳态和茉莉酸信号传导增强甘蓝型油菜对菌核菌的抗性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-24 DOI: 10.1016/j.plaphy.2024.109446

Jinxiong Xu, Huanhuan Jiang, Qing Cao, Yali Li, Xianjue Kuang, Yulun Wu, Yourong Chai, Jiana Li, Kun Lu, Lijuan Wei

{"title":"The glutathione S-transferase BnGSTU12 enhances the resistance of Brassica napus to Sclerotinia sclerotiorum through reactive oxygen species homeostasis and jasmonic acid signaling.","authors":"Jinxiong Xu, Huanhuan Jiang, Qing Cao, Yali Li, Xianjue Kuang, Yulun Wu, Yourong Chai, Jiana Li, Kun Lu, Lijuan Wei","doi":"10.1016/j.plaphy.2024.109446","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109446","url":null,"abstract":"Sclerotinia sclerotiorum is a severe disease that affects rapeseed (Brassica napus), resulting in significant yield losses. In previous study, we identified the candidate GLUTATHIONE S-TRANSFERASE (GST) gene, BnGSTU12, associated with sclerotiorum stem resistance and the expression levels of BnGSTU12 in resistant lines were higher than that in susceptible lines. We analyzed the function of the BnGSTU12 during S. sclerotiorum infection in this study. BnGSTU12 expression was induced by S. sclerotiorum, with a strong increase 24 h after onset of infection. Transgenic functional analysis indicated that overexpression of BnGSTU12 in Arabidopsis thaliana and B. napus enhanced resistance to S. sclerotiorum, whereas BnGSTU12 silencing decreased S. sclerotiorum resistance. The inoculated BnGSTU12-OE A. thaliana and B. napus plants showed higher antioxidant enzyme activity and lower H2O2 contents than the wild type. As BnGSTU12 was rapidly induced by the phytohormones salicylic acid (SA), ethylene, and methyl jasmonate (MeJA), we investigated the involvement of the JA and SA pathways in GSTU12-mediated S. sclerotiorum resistance. JA content was higher in infected BnGSTU12-OE plants than in the wild type, whereas their SA contents were comparable. In addition, the expression levels of JASMONATE RESISTANT (JAR) involved in JA-Ile biosynthesis and those of JA-responsive genes were higher, the expression of JAZ gene repressing JA signaling was less in OE plants than WT after 12 and 24 h inoculation with S. sclerotiorum. Our results show that BnGSTU12 enhances resistance to S. sclerotiorum through ROS homeostasis and JA signaling.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109446"},"PeriodicalIF":6.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thriving in adversity: Understanding how maize seeds respond to the challenge of combined cold and high humidity stress. 逆境中茁壮成长：了解玉米种子如何应对寒冷和高湿双重压力的挑战。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-24 DOI: 10.1016/j.plaphy.2024.109445

Xiangzeng Meng, Denglong Chen, Yanjie Lv, Wenhua Xu, Yongjun Wang, Lichun Wang

{"title":"Thriving in adversity: Understanding how maize seeds respond to the challenge of combined cold and high humidity stress.","authors":"Xiangzeng Meng, Denglong Chen, Yanjie Lv, Wenhua Xu, Yongjun Wang, Lichun Wang","doi":"10.1016/j.plaphy.2024.109445","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109445","url":null,"abstract":"Extreme conditions, such as cold and high humidity in northeast China's high-latitude maize region, can hinder crop yield and stability during the vegetative stage. However, there is a paucity of research examining the effects of simultaneous cold and high humidity stress on plant responses. In this study, we characterized the acclimation of JD558 (cold- and high humidity-sensitive hybrid) and JD441 (cold- and high humidity-tolerant hybrid) to stress at sowing caused by cold (4 °C), high humidity (25%), and their combined stress for five days, using physiological measurements and metabolomics during the stress treatments and recovery stages. Cold, high humidity, and their combined stress prolonged seed development and restricted material transport, with high humidity harming seed survival more than cold. Combined stress exhibited a more significant inhibitory effect on growth than individual stress. Individual and combined stress reduced α-amylase activity, disrupted antioxidants levels, increased malondialdehyde content, disturbed the oxidative balance within seeds, and impeded seed growth and development. Most carboxylic acids and their derivatives were downregulated caused by combined stress. In JD558, sucrose, D-glucose, glucose-1-phosphate, and fructose-1,6-bisphosphate were downregulated, while these metabolites were upregulated in JD441, leading to a blockage of glycolysis in JD558. After eliminating stress, JD441 showed greater α-amylase activity and a smaller decrease in MDA levels, resulting in a smaller reduction in root growth and transport rate than JD558. In summary, the different responses of the cold and high humidity sensitive hybrid and the tolerant hybrid to combined stress are related to the recovery ability after stress elimination.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109445"},"PeriodicalIF":6.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics analysis reveals crucial effects of arbuscular mycorrhizal fungi on the metabolism of quality compounds in shoots and roots of Camellia sinensis L. 代谢组学分析揭示了丛枝菌根真菌对茶树芽和根中优质化合物代谢的重要影响。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-22 DOI: 10.1016/j.plaphy.2024.109426

Shibei Ge, Zheng Zhang, Qiang Hu, Qiuhong Wang, Xuejiao Gong, Fan Huang, Lan Zhang, Wenyan Han, Fan Luo, Xin Li

{"title":"Metabolomics analysis reveals crucial effects of arbuscular mycorrhizal fungi on the metabolism of quality compounds in shoots and roots of Camellia sinensis L.","authors":"Shibei Ge, Zheng Zhang, Qiang Hu, Qiuhong Wang, Xuejiao Gong, Fan Huang, Lan Zhang, Wenyan Han, Fan Luo, Xin Li","doi":"10.1016/j.plaphy.2024.109426","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109426","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are known as plants' mutualists to enhance plant growth, but their impact on the quality-related metabolites in Camellia sinensis still needs to be studied. In this study, the 2-year-old potted C. sinensis cv. 'Longjing 43' was inoculated with AMF Rhizophagus irregularis to examine the effect of AMF colonization for 3 months on plant growth, photosynthesis, and changes in metabolomics and associated gene expression in the shoots and roots of tea plants. The results showed that AMF not only promoted the growth of tea plants but also significantly up-regulated the total contents of flavonoids and free amino acids, especially the anthocyanins, flavanols, GABA, and arginine. Consistently, the expression of genes such as F3H, DFR, LAR, ANR, UFGT, GDH, and GS in tea shoots was induced by AMF. Further studies found that transcription factors MYBs and HY5, as well as phytohormone strigolactones, were induced by AMF, which may participate in the regulatory mechanism controlling the metabolism of tea-quality compounds. These findings revealed regulatory mechanisms through which AMF affected tea quality and provided a theoretical basis for the application of AMF in tea gardens to improve the economic value and health benefits of tea.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109426"},"PeriodicalIF":6.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Metals and metalloids toxicity at the plant-soil interface and novel strategies to mitigate their effects. 社论：金属和类金属在植物-土壤界面的毒性和减轻其影响的新策略。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-21 DOI: 10.1016/j.plaphy.2024.109438

M Nasir Khan, Manzer H Siddiqui, Izabela Natalia Jośko, M Naeem

引用次数: 0

Protective effect of Ocimum basilicum L. essential oil on Lactuca sativa L. treated with cadmium. 罗勒精油对镉胁迫下芥蓝的保护作用。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-21 DOI: 10.1016/j.plaphy.2024.109437

Simone Landi, Flavio Polito, Sergio Esposito, Sergio Sorbo, Piergiorgio Cianciullo, Alessia Postiglione, Vincenzo De Feo, Adriana Basile, Viviana Maresca

{"title":"Protective effect of Ocimum basilicum L. essential oil on Lactuca sativa L. treated with cadmium.","authors":"Simone Landi, Flavio Polito, Sergio Esposito, Sergio Sorbo, Piergiorgio Cianciullo, Alessia Postiglione, Vincenzo De Feo, Adriana Basile, Viviana Maresca","doi":"10.1016/j.plaphy.2024.109437","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109437","url":null,"abstract":"In recent years, essential oils (EO) are a sustainable and effective alternative to conventional chemical treatments in response to heavy metals in plants. These natural molecules can increase the resilience of plants under stress conditions. In the present work, the ability of EOs from the aerial parts of Ocimum basilicum L. cv 'Prospera' to improve plant response to heavy metals in Lactuca sativa L. grown hydroponically and subjected to Cd stress was investigated. The chemical profile of the essential oil (EO) was analyzed by GC-MS. Essential oil-induced tolerance to different Cd concentrations (36 μM and 72 μM) was studied by analyzing ultrastructural damage by TEM observations, antioxidant response by spectrophotometric analysis, and changes in gene expression by qRT-PCR involved in abiotic stress response. Our results indicated that exogenous EO application of basil helps preserve plastid ultrastructure and ameliorates Cd-induced damage. In addition, there was a reduction in ROS production and beneficial regulation of the activities and molecular expression of antioxidant enzymes. In conclusion, these results clearly indicate the protective ability of basil EO on cytological organization and in modulating the redox state through the antioxidant pathway, reducing Cd-induced oxidative stress.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109437"},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput identification of Prunus mume freezing-tolerance genes based on yeast screening system and functional verification of PmRCI2-3 in Arabidopsis. 基于酵母筛选系统的拟南芥李抗冻基因高通量鉴定及PmRCI2-3功能验证

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-20 DOI: 10.1016/j.plaphy.2024.109431

Lichen Yang, Xue Li, Ke Chen, Zhiyuan Ma, Like Qiu, Chongyang Yuan, Jia Wang, Tangren Cheng, Qixiang Zhang, Tangchun Zheng

{"title":"High-throughput identification of Prunus mume freezing-tolerance genes based on yeast screening system and functional verification of PmRCI2-3 in Arabidopsis.","authors":"Lichen Yang, Xue Li, Ke Chen, Zhiyuan Ma, Like Qiu, Chongyang Yuan, Jia Wang, Tangren Cheng, Qixiang Zhang, Tangchun Zheng","doi":"10.1016/j.plaphy.2024.109431","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109431","url":null,"abstract":"Prunus mume tops the ten most famous flowers of China with high ornamental value, and low temperature is the main factor limiting its northward migration. Cold resistance improvement is one of the important breeding directions of Rosaceae ornamental plants, especially the Prunus mume. Here, 29 genes from P. mume were screened by yeast screening system under -20 °C for 96 h. Based on GO and KEGG analysis, rare cold-inducible 2 family gene member PmRCI2-3 was first cloned for functional verification. Subcellular localization results showed the PmRCI2-3 was located in the membrane structure, and GUS staining showed that the activity of the PmRCI2-3 promoter was spatiotemporally specific. Overexpression PmRCI2-3 in Arabidopsis thaliana can reduce plant damage at low temperatures. The expression levels of endogenous genes (AtCBF1, AtCBF2, AtCBF3, AtCOR15A, and AtRD29A) related to cold response were all up-regulated, except AtKIN was down-regulated. These results lay the foundation for further providing key candidate genes for cold resistance breeding of P. mume and other Prunus species.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109431"},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing mRNA-meth-miRNA single-sample networks to reveal the molecular interaction patterns induced by lunar orbital stressors in rice (Oryzasativa). 构建mrna -甲基- mirna单样本网络，揭示月轨胁迫诱导水稻分子相互作用模式。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-20 DOI: 10.1016/j.plaphy.2024.109430

Yan Zhang, Xiaohui Du, Meng Zhang, Yeqing Sun

{"title":"Constructing mRNA-meth-miRNA single-sample networks to reveal the molecular interaction patterns induced by lunar orbital stressors in rice (Oryzasativa).","authors":"Yan Zhang, Xiaohui Du, Meng Zhang, Yeqing Sun","doi":"10.1016/j.plaphy.2024.109430","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109430","url":null,"abstract":"To explore the bio-effects during Moon exploration missions, we utilized the Chang'E 5 probe to carry the seeds of Oryza. Sativa L., which were later returned to Earth after 23 days in lunar orbit and planted in an artificial climate chamber. Compared to the control group, rice seeds that underwent spaceflight showed inhibited growth and development when planted on the ground. Then we collected samples and employed RNA sequencing (RNA-Seq) and whole-genome bisulfite sequencing (WGBS) in the tillering and heading stages of rice. To gain a comprehensive understanding of the dysregulation in molecular interaction patterns during Moon exploration, a bioinformatics pipeline based on mRNA-meth-miRNA Single-Sample Networks (SSNs) was developed. Specifically, we constructed four SSNs for each sample at the mRNA, DNA methylation (promoter and gene bodies), and miRNA levels. By combining with the Protein-Protein Interaction (PPI) network, SSNs can character individual-specific gene interaction patterns. Under spaceflight conditions, distinct interaction patterns emerge across various omics levels. However, the molecules driving changes at each omics level predominantly regulate consistent biological functions, such as metabolic processes, DNA damage and repair, cell cycle, developmental processes, etc. In the tillering stage, pathways such as ubiquitin mediated proteolysis, nucleotide excision repair, and nucleotide metabolism are significantly enriched. Moreover, we identified 18 genes that played key/hub roles in the dysregulation of multi-omics molecular interaction patterns, and observed their involvement in regulating the above biological processes. As aforementioned, our multi-omics SSNs method can reveal the molecular interaction patterns under deep space exploration.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109430"},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PsbHLH58 positively regulates sucrose accumulation by modulating Sucrose synthase 4 in 'Fengtang' plum (Prunus salicina Lindl.). pbhlh58通过调节丰塘李蔗糖合酶4正向调节蔗糖积累。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-20 DOI: 10.1016/j.plaphy.2024.109435

Qiyu Wang, Xiaoshuang Nie, Kangze Luo, Hong Chen, Min Lu, Huaming An

{"title":"PsbHLH58 positively regulates sucrose accumulation by modulating Sucrose synthase 4 in 'Fengtang' plum (Prunus salicina Lindl.).","authors":"Qiyu Wang, Xiaoshuang Nie, Kangze Luo, Hong Chen, Min Lu, Huaming An","doi":"10.1016/j.plaphy.2024.109435","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109435","url":null,"abstract":"Sugar content is an important factor that largely determines fruit quality. 'Fengtang' plum (Prunus salicina Lindl.) is recognized for its high soluble sugar content, and the Sucrose synthase 4 (PsSUS4) functions as the controlling step in sucrose accumulation. Nevertheless, the transcriptional mechanism of PsSUS4 underlying sucrose production in this high-sugar plum remains unclear. In this work, a bHLH transcription factor PsbHLH58 was identified to be positively correlated with PsSUS4 expression and fruit sucrose content based on the transcriptome data and qRT-PCR validation. Further yeast one-hybrid and dual-luciferase assays confirmed that PsbHLH58 acts as a transcriptional activator of PsSUS4 via specifically binding to its promoter. Manipulation of the PsbHLH58 expression in fruits through transient overexpression and gene silencing resulted in significant corresponding changes in PsSUS4 expression and sucrose accumulation. In addition, it was also found that the expression levels of PsbHLH58 and PsSUS4 are positively regulated by ethylene, accompanied by an increased sugar accumulation in the fruits. Consequently, these results demonstrated a novel mechanism involving the PsbHLH58-PsSUS4 module that mediates the sugar accumulation in 'Fengtang' plum and provided a new insight into the future fruit quality improvement.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109435"},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0