Plant Physiology and Biochemistry最新文献

{"title":"Chlorophyll fluorescence characteristics and lipid metabolism in endangered Cycas panzhihuaensis exposed to drought, high temperature and their combination1","authors":"Jiao Yu ,&nbsp;Fang Wang ,&nbsp;Aiguo Jiang,&nbsp;Miaomiao Hu,&nbsp;Yanling Zheng","doi":"10.1016/j.plaphy.2025.109858","DOIUrl":"10.1016/j.plaphy.2025.109858","url":null,"abstract":"<div><div><em>Cycas panzhihuaensis</em>, an endangered species distributed in the dry-hot valleys of southwestern China, faces drought (D), heat (H), and their combination (DH) under current and future climatic conditions. To explore the responses of <em>C. panzhihuaensis</em> to D, H, and DH, chlorophyll fluorescence and the lipid and fatty acid profiles were determined. Leaf water loss and leaf damage only occurred following DH treatment. The photochemical activity was least impacted by D stress and most severely impacted by DH stress. D treatment reduced the levels of most lipid categories and total fatty acids. Both the H and DH treatments led to a significant decrease in the levels of saccharolipids, lysophospholipids, sphingolipids, and fatty acyls, while significantly increasing the levels of neutral glycerolipids and fatty acids. Moreover, odd-numbered fatty acids and <em>trans-</em>fatty acids-C18:2ttn-6 accumulated significantly following both H and DH treatments. However, the levels of both total fatty acids and total lipids were significantly lower after DH stress compared to H stress. The proportion of saturated fatty acids increased after D treatment and that of polyunsaturated fatty acids increased after both H and DH treatments. Following various treatments, the degree of unsaturation in total phospholipids decreased, while that in total saccharolipids remained unchanged. Additionally, the unsaturation levels of diacylglycerol and triacylglycerol showed no change after D stress, but increased after H and DH treatments. In conclusion, <em>C. panzhihuaensis</em> exhibited varying levels of tolerance to D, H, and DH treatments, which may be related to the differential adjustments in lipid composition and unsaturation levels.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109858"},"PeriodicalIF":6.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of alfalfa flavonol synthase genes and functional identification of MsFLS13 in response to cold stress","authors":"Lishuang Zhang ,&nbsp;Yang Ding ,&nbsp;Haimei Dong,&nbsp;Lei Liu,&nbsp;Jinqiang Ji,&nbsp;Changhong Guo","doi":"10.1016/j.plaphy.2025.109871","DOIUrl":"10.1016/j.plaphy.2025.109871","url":null,"abstract":"<div><div>Flavonol synthase (FLS) plays a vital role in flavonol biosynthesis in plants, crucial in their growth, development, and ability to withstand abiotic stress. However, a comprehensive analysis of the FLS gene family and its role in alfalfa (<em>Medicago sativa</em> L.) under cold stress remains unexplored. Therefore, this study aims to employ bioinformatics methods, integrating various databases and computational tools, to systematically investigate the <em>MsFLS</em>s gene family across the entire alfalfa (<em>Medicago sativa</em> L) genome. Furthermore, qRT-PCR experiments were performed to validate expression patterns. Twenty <em>MsFLS</em> genes were identified and classified into five distinct subgroups based on their phylogenetic trees. Gene structure analysis revealed that alfalfa genes contained between one and five introns. The number of introns within members of the same evolutionary branch was generally consistent. The <em>MsFLS</em> promoter region contained a substantial number of hormone-responsive, stress-responsive, light-responsive, and tissue-specific regulatory elements. Additionally, approximately 95 % (19/20) of the alfalfa FLS genes underwent duplication events involving tandem and fragment replications across 47 replication events. Cold stress triggered the expression of the <em>MsFLS</em> gene family, with <em>MsFLS7</em>, <em>MsFLS9</em>, <em>MsFLS10</em>, <em>MsFLS11</em>, <em>MsFLS13</em>, <em>MsFLS16</em>, <em>MsFLS17</em> and <em>MsFLS18</em> showing significant upregulation. The overexpression of <em>MsFLS13</em> significantly improved cold stress tolerance and antioxidant capacity and reduced membrane oxidative damage in alfalfa. These findings offer valuable insights for future research on the functional role of <em>MsFLS</em> genes in response to cold stress in alfalfa.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109871"},"PeriodicalIF":6.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A naturally integrated RolD-like gene in sweet potato mediates stress-responsive pathways","authors":"Yulia Yugay,&nbsp;Tatiana Rusapetova,&nbsp;Elena Vasyutkina,&nbsp;Maria Sorokina,&nbsp;Valeria Grigorchuk,&nbsp;Veronika Degtyareva,&nbsp;Dina Rudenko,&nbsp;Egor Alaverdov,&nbsp;Victor Bulgakov,&nbsp;Yury Shkryl","doi":"10.1016/j.plaphy.2025.109875","DOIUrl":"10.1016/j.plaphy.2025.109875","url":null,"abstract":"<div><div>Sweet potato (<em>Ipomoea batatas</em>), a globally significant staple crop, exhibits remarkable adaptability to various environmental conditions, largely due to its genetic diversity. Recent studies have revealed the presence of naturally integrated <em>Agrobacterium</em> cellular T-DNAs (cT-DNAs) within the sweet potato genome, suggesting their possible role in the evolution and adaptation of sweet potato. In this study, we characterize a newly identified open reading frame (ORF) within the cT-DNA2 region of <em>I. batatas</em>, which encodes a homolog of the <em>A. rhizogenes rolD</em> gene. This ORF encodes a RolD-like protein with ornithine cyclodeaminase (OCD) activity, a key enzyme in proline biosynthesis. Functional assays confirmed that the recombinant RolD-like protein exhibits ornithine-dependent NAD⁺ reduction, similar to the product of the <em>rolD</em> gene. Notably, <em>rolD-like</em> gene expression was strongly up-regulated by methyl jasmonate treatment, as well as in response to abiotic stresses such as heat, cold, salt, drought, high light, and UV radiation. Overexpression of this <em>rolD-like</em> gene in <em>Arabidopsis thaliana</em> resulted in delayed flowering, shortened siliques, and reduced seed production, along with enhanced proline accumulation, indicating its role in stress response mechanisms. These findings suggest that the natural integration of this <em>rolD-like</em> gene may contribute to the sweet potato's resilience to abiotic stresses, offering potential for the development of improved cultivars with enhanced stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109875"},"PeriodicalIF":6.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-methylation modifications in the biosynthetic pathway of dibenzocyclooctadiene lignans","authors":"Meiyu Duan ,&nbsp;Run Yang ,&nbsp;Yina Wang ,&nbsp;Yongkang Zhang ,&nbsp;Guisheng Xiang ,&nbsp;Lei Feng ,&nbsp;Xiangyu Liu ,&nbsp;Fengling Tan ,&nbsp;Feifei Wang ,&nbsp;Yan Zhao ,&nbsp;Bing Hao ,&nbsp;Guanghui Zhang ,&nbsp;Shengchao Yang","doi":"10.1016/j.plaphy.2025.109863","DOIUrl":"10.1016/j.plaphy.2025.109863","url":null,"abstract":"<div><div><em>Schisandra chinensis</em>, a well-known traditional Chinese herb used for hepatitis treatment, contains dibenzocyclooctadiene lignans as its primary active compounds, which undergo extensive multi-site O-methylation. However, <em>O</em>-methyltransferases (OMT) involved in this process have not been previously reported. This study employed transcriptomic analysis of <em>S. chinensis</em> treated with methyl jasmonate, alongside expression profiling, phylogenetic analysis, and heterologous expression to characterize the functional roles of OMTs. The study identified 4 OMTs: SchiOMT4, SchiOMT12, SchiOMT16, and SchiOMT22, which catalyze C-3 O-methylation of caffeic acid and Caffeyl aldehyde to form ferulic acid and coniferyl aldehyde. Additionally, SchiOMT12 and SchiOMT16 methylated gomisin L2 at C-3, while SchiOMT16 also O-methylation schisanhenol at C-14 and performed sequential O-methylation at C-3 and C-12 of gomisin J. Molecular docking further clarified the regioselectivity of SchiOMT16 and SchiOMT12, elucidating the differences in their catalytic activities. This study is the first to identify methyltransferases involved in the subsequent modifications of dibenzocyclooctadiene lignans, underscoring the broad substrate range, selectiv<em>e O</em>-methylation, and regulatory importance of OMTs in their biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109863"},"PeriodicalIF":6.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FvbHLH78 interacts with FvCRY2 to promote flowering in woodland strawberry","authors":"Jinxiang Yao ,&nbsp;Shuo Zhao ,&nbsp;Yuxin Nie,&nbsp;Zhengjia Wu,&nbsp;Junxiang Zhang,&nbsp;Zhihong Zhang","doi":"10.1016/j.plaphy.2025.109856","DOIUrl":"10.1016/j.plaphy.2025.109856","url":null,"abstract":"<div><div>Flowering is a crucial agricultural trait of strawberries. While the bHLH family comprises numerous members in plants, its function in controlling strawberry flowering remains largely unexplored. In this study, <em>FvbHLH78</em> was found to be highly expressed in the shoot apices and ripening fruits of woodland strawberry (<em>Fragaria vesca</em>). FvbHLH78 is localized to the nucleus and exhibits self-activating transcriptional properties. Overexpression of <em>FvbHLH78</em> in woodland strawberry resulted in an early flowering phenotype compared to the control plants. This phenomenon was attributed to FvbHLH78 directly binding to the promoters of the genes associated with flowering, namely <em>FvFT</em>, <em>FvSEP3</em>, <em>FvLFY</em>, and <em>FvAGL42</em>. Moreover, FvbHLH78 interacted with a blue light receptor FvCRY2, which enhances FvbHLH78 promoter-binding affinity to <em>FvFT</em>, <em>FvSEP3</em>, <em>FvLFY</em>, and <em>FvAGL42</em>, thereby accelerating flowering. Collectively, these findings demonstrate that the FvbHLH78-FvCRY2 complex in strawberries acts as an enhancer of genes associated with flowering, thereby accelerating the flowering process. These data offer an understanding for enriching the roles of bHLH78 and accelerating flowering in strawberry.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109856"},"PeriodicalIF":6.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of miRNA-mRNA regulatory network during the germination of soybean seed (Glycine max) and the role of Gma-miR1512a-GmKIN10 interaction","authors":"Yiqiang Han ,&nbsp;Hongyan Zhao ,&nbsp;Yamei Gao ,&nbsp;Haonan Chen ,&nbsp;Jidao Du ,&nbsp;Zheng Hu","doi":"10.1016/j.plaphy.2025.109853","DOIUrl":"10.1016/j.plaphy.2025.109853","url":null,"abstract":"<div><div>Seed germination is a key and complex physiological process in plant life, including soybeans. Here, we explored the miRNA-mRNA transcriptome changes and the key genes in the germination stages of the soybean. Morphological analysis showed that the imbibition of seeds was completed at 12 h, and the embryo broke through the seed coat at 36 h. During seed germination, mRNA and miRNA sequencing identified 20845 differentially expressed mRNAs (DEMs) and 421 differentially expressed miRNAs (DEMIs) at three specific time points: 12 h, 36 h, and 108 h. KEGG enrichment revealed that plant hormone signal transduction, plant-pathogen interaction and MAPK signaling pathway-plant were the crucial biological processes for seed germination. ABA and GA related DEMs on plant hormone signal transduction were abundant. miRNA-mRNA integrated analysis showed that 5170 miRNA-mRNA pairs were found. During germination, 20 significant miRNA-mRNA interactions were identified, involving the top 10 differentially expressed miRNAs (DEMIs) and 198 differentially expressed mRNAs (DEMs). Interestingly, the expression level of <em>Gma-miR1512a</em> increased significantly during soybean seed germination. This miRNA specifically regulates <em>GmKIN10</em>, homologous to <em>AtKIN10</em>, which mediates germination. To verify this interaction, co-agroinjection of <em>GmKIN10</em>-GFP/GUS and <em>Gma-miR1512a</em> into tobacco leaves demonstrated that <em>Gma-miR1512a</em> can inhibit <em>GmKIN10</em> expression by cleaving its target site. Furthermore, the function of <em>Gma-miR1512a-GmKIN10</em> were verified by overexpression transgene. Although Arabidopsis seeds overexpressing <em>Gma-miR1512a</em> (OE-<em>Gma-miR1512a</em>) showed no significant differences in germination indices compared to wild-type (WT) seeds, those overexpressing <em>GmKIN10</em> (OE<em>-GmKIN10</em>) exhibited significantly lower germination indices. The seeds germination index of <em>GmKIN10</em> and <em>Gma-miR1512a</em> double overexpression lines recovered. Additionally, the yeast two-hybrid assay, protein interaction prediction,and molecular docking all showed that GmKIN10 might interact with GmPP2A and GmDSP4. This study identified a complex miRNA-mRNA regulatory network that plays a crucial role in soybean seed germination. Specifically, <em>Gma-miR1512a</em> was found to regulate <em>GmKIN10</em>, significantly influencing germination rates and hormone signaling pathways.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109853"},"PeriodicalIF":6.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CitUNE1 inhibits (+)-valencene synthesis by regulating CsTPS1 in ‘Newhall’ sweet orange","authors":"Shuling Shen ,&nbsp;Yuwei Zhou ,&nbsp;Mengyao Yin ,&nbsp;Sijia Liu ,&nbsp;Hui Sun ,&nbsp;Yue Guan ,&nbsp;Chen Huan ,&nbsp;Xiaolin Zheng","doi":"10.1016/j.plaphy.2025.109854","DOIUrl":"10.1016/j.plaphy.2025.109854","url":null,"abstract":"<div><div>(+)-Valencene is the characteristic volatile compound in ‘Newhall’ sweet orange, and <em>CsTPS1</em> is the gene that codes for the (+)-valencene synthase. Here, four transcription factors, including CitUNE1, CitUNE3, CitSCL1, and CitSCL13, were screened as candidate proteins by yeast one-hybrid (Y1H) library screening with <em>CsTPS1</em> promoter as the bait. Among them, CitUNE1 bound to the G-box on the promoter of <em>CsTPS1</em> and suppressed <em>CsTPS1</em> expression, confirmed by Y1H, dual-luciferase assay, point-mutation experiment and EMSA. The expression pattern of <em>CitUNE1</em> showed a negative correlation with both the content of (+)-valencene and <em>CsTPS1</em> transcripts level, both during fruit development and after ethylene treatment. Furthermore, the role of CitUNE1 in (+)-valencene synthesis was confirmed using the transient over-expression and silencing in ‘Newhall’ sweet orange. Transient over-expression of CitUNE1 inhibited <em>CsTPS1</em> expression and reduced the accumulation of (+)-valencene, while silencing of <em>CitUNE1</em> induced <em>CsTPS1</em> expression and triggered (+)-valencene synthesis in ‘Newhall’ sweet orange.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109854"},"PeriodicalIF":6.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promiscuous and regiospecific Vinca minor hydroxylases for opioid akuammine biosynthesis and monoterpenoid indole alkaloid diversification","authors":"Zhan Mai ,&nbsp;Matthew Bailey Richardson ,&nbsp;Scott Galeung Alexander Mann,&nbsp;Julia Greene,&nbsp;Allyson Audrey Paul,&nbsp;Jacob Owen Perley,&nbsp;Ghislain Deslongchamps,&nbsp;Yang Qu","doi":"10.1016/j.plaphy.2025.109841","DOIUrl":"10.1016/j.plaphy.2025.109841","url":null,"abstract":"<div><div>The medicinal plant <em>Vinca minor</em> produces vincamine, a compound used for neurodegenerative diseases, along with a diverse array of monoterpenoid indole alkaloids (MIAs) primarily within the aspidosperma and akuammiline subclasses. While recent studies have elucidated the core biosynthetic pathways for these subclasses, the transformations of key intermediates into the vast diversity of naturally occurring alkaloids remain poorly understood. In this study, we identify and characterize two promiscuous cytochrome P450 monooxygenases (CYPs) in <em>V. minor</em>: vincaminoreine/pericyclivine 10-hydroxylase (VmV10H) and pseudoakuammigine 10-hydroxylase (VmPs10H), both exhibiting high substrate versatility. VmV10H catalyzes the hydroxylation of structurally diverse MIAs, including vincaminoreine, pericyclivine, apparicine, and akuammidine, while VmPs10H demonstrates a preference for akuammiline type MIAs such as pseudoakuammigine, picrinine, and strictamine. Homology modeling and substrate docking reveal active site architecture of these enzymes, suggesting a consistent mechanism for C10 hydroxylation across all substrates. The discovery of VmV10H and VmPs10H not only broadens our understanding of MIA biosynthesis but also expands the enzymatic toolkit for the metabolic engineering of pharmaceutical MIAs, including akuammine, a μ-opioid receptor agonist with analgesic properties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109841"},"PeriodicalIF":6.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pumpkin heat shock factor CmHSF30 positively regulates thermotolerance in transgenic plants","authors":"Bobo Liu ,&nbsp;Long Li ,&nbsp;Ganxiyu Cheng ,&nbsp;Fengmei Li ,&nbsp;Shuxia Zhang","doi":"10.1016/j.plaphy.2025.109834","DOIUrl":"10.1016/j.plaphy.2025.109834","url":null,"abstract":"<div><div>Heat shock factors (HSFs) play a central role in regulating the responses of plants to various stresses. However, the function and regulation of HSFs in pumpkins remains largely unknown. In this study, an HSF, <em>CmHSF30</em> was identified in <em>C</em><em>ucurbia</em> <em>moschata</em>, which belongs to the HSFA subfamily. The expression level of <em>CmHSF30</em> was significantly upregulated in response to heat stress and exogenous phytohormone treatments, including ABA, GA, IAA, and SA. The <em>Cm</em>HSF30 was localized in the nucleus and functions as a transcriptional activator. By overexpressing <em>CmHSF30</em> in <em>Arabidopsis</em> and pumpkin, the function and regulation of <em>CmHSF30</em> in response to heat stress were studied. The overexpression of <em>CmHSF30</em> in <em>Arabidopsis</em> enhanced plant thermotolerance by increased germination rate and survival rate under heat stress, as evidenced by the elevated of contents chlorophyll and GSH, and SOD activity, and decreased contents of H₂O₂ and MDA. Furthermore, the overexpression of <em>CmHSF30</em> in pumpkins also enhanced the thermotolerance of transgenic pumpkins by reducing cell death. In contrast, CRISPR/Cas9 mediated knockout of <em>CmHSF30</em> decreased pumpkin thermotolerance. Besides, RT-qPCR analysis revealed that <em>CmHSF30</em> plays a positive role in regulating the expression of stress-related genes, including <em>AtHSP18.2</em>, <em>AtHSP20</em>, <em>AtHSP70</em>, <em>AtPP2C</em>, and <em>AtMYB82</em> from <em>Arabidopsis</em> and <em>CmHSP18.2</em>, <em>CmHSP20</em>, <em>CmHSP70</em>, <em>CmPP2C</em>, and <em>CmMYB46</em> from pumpkin. Yeast two-hybrid showed that <em>Cm</em>HSF30 interacts with <em>Cm</em>MYB46. The results indicate that <em>Cm</em>HSF30 functions as a positive regulator, enhancing plant thermotolerance by regulating target genes and reducing ROS accumulation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109834"},"PeriodicalIF":6.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating effects of Methyl Jasmonate on photosynthetic inhibition and oxidative stress of pepper (Capsicum annuum L) seedlings under low temperature combined with low light","authors":"Kaiguo Pu,&nbsp;Nenghui Li,&nbsp;Yanqiang Gao,&nbsp;Tiantian Wang,&nbsp;Miao Zhang,&nbsp;Wenli Sun,&nbsp;Jing Li,&nbsp;Jianming Xie","doi":"10.1016/j.plaphy.2025.109843","DOIUrl":"10.1016/j.plaphy.2025.109843","url":null,"abstract":"<div><div>Low temperature combined with low light (LL) is a critical abiotic stress that restricting plant growth and yield of pepper (<em>Capsicum annuum</em> L.). Methyl jasmonate (MeJA) is considered with potential benefits for improving plant stress resistance; however, the physiological mechanisms underlying the adaptation of pepper to LL stress have not been explored. This study aimed to investigate the potential mitigating effects of foliar MeJA (200 μmol L⁻¹) application on pepper seedlings subjected to LL stress (10/5 °C, 100 μmol m⁻² s⁻¹) for 168 h. Our results indicated that the application of exogenous MeJA reduced the negative effect on growth inhibition of pepper seedlings caused by LL stress, significantly increased chlorophyll contents and photosynthetic capacity as a result of improved photosynthesis rate. In addition, MeJA reduced the accumulation of reactive oxygen species and malondialdehyde contents induced by LL stress, while enhancing the activities of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase as a result of upregulated expression levels of antioxidant enzyme genes (<em>CaSOD, CaPOD, CaCAT, CaAPX, CaGR, CaDHAR</em>, and <em>CaMDHAR</em>). Additionally, it increased the ascorbic acid and reduced glutathione content, while reducing oxidized glutathione content, thereby preventing membrane lipid peroxidation and protecting plants from oxidative damage under LL stress. Furthermore, seedlings treated with MeJA exhibited significantly enhanced soluble sugar and soluble protein contents in leaves. Taken together, present findings indicate that MeJA application may serve as an effective strategy for mitigating LL-induced oxidative stress by maintaining plant growth, enhancing chlorophyll fluorescence, upregulating the antioxidant defence system, optimizing ascorbate-glutathione cycle, and osmotic adjustment.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109843"},"PeriodicalIF":6.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}