Plant Physiology and Biochemistry最新文献

{"title":"Metabolic profiling of Achillea millefolium from the Chernobyl exclusion zone reveals the adaptive strategies to low-dose chronic radiation exposure.","authors":"Sofia Bitarishvili, Gilles Clement, Christian Meyer, Polina Volkova","doi":"10.1016/j.plaphy.2025.109551","DOIUrl":"10.1016/j.plaphy.2025.109551","url":null,"abstract":"<p><p>The radionuclide contamination of the environment is an abiotic stress factor that influences biological systems. Plants growing in contaminated areas for many generations provide a unique opportunity to study adaptive strategies aimed at maintaining homeostasis under elevated radiation levels. Using non-targeted metabolomics approaches, we investigated the metabolomic profiles of Achillea millefolium L. plants from the Chernobyl exclusion zone. Amino acid biosynthesis pathways (arginine, glycine, serine, threonine, and proline) and metabolites associated with nitrogen mobilization, cell wall response to injury, photosynthetic efficiency, and defence responses were highly affected in plants from contaminated plots. Our results suggest that these changes may be involved in the adaptive strategies of A. millefolium plant to chronic radiation exposure.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109551"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth-promoting effects and possible mechanisms of a plant endophytic fungus Aureobasidium sp. JRF1","authors":"Zexuan Jiang ,&nbsp;Fangren Peng ,&nbsp;Jinping Yu ,&nbsp;Qi Li","doi":"10.1016/j.plaphy.2025.109724","DOIUrl":"10.1016/j.plaphy.2025.109724","url":null,"abstract":"<div><div>Endophytic fungi can establish symbiosis with host plant and promote plant growth in a sustainable way. In this study, a previously-isolated plant growth-promoting endophytic fungus JRF1 was deeply studied. JRF1 could colonize <em>Arabidopsis</em> and tomato seedlings and promote their growth. Through sequencing the internal transcribed spacer (ITS) region and <em>18S rRNA</em> gene, JRF1 was identified as an <em>Aureobasidium</em> sp. strain. Transcriptome analysis indicated that JRF1 treatment up-regulated a majority of genes related to calcium signal and genes annotated as peroxidase and glutathione S- transferase. In addition, treatment with JRF1 activated the Aux/IAA (auxin/indole acetic acid) and cytokinin signaling, while down-regulated genes involved in JA/ETH (jasmonic acid/ethylene) pathways. Split co-culture assay not only demonstrated that JRF1 significantly promoted the <em>Arabidopsis</em> growth by direct contacting the seedlings, but also suggested JRF1 could exhibit positive effects in a non-contact manner. Subsequently, metabolome analysis revealed that JRF1 produced many soluble metabolites which might be responsible for plant growth-promoting, and the releasing volatile organic compounds (VOCs) of JRF1 was also isolated and detected. Finally, we found that both cell-free supernatant (CFS) of JRF1 and its fermentation solution could outstandingly promote the plant growth, suggesting its possible role as a microbial fertilizer. Our results uncovered the interaction mode between JRF1 and host plant, proposing that the combined action of JRF1 with its metabolites resulted in the enhanced plant growth<strong>.</strong></div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109724"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel study on enhancing ascorbic acid colorimetric detection: Green synthesis-driven crystallinity, stability, and catalytic performance of iron oxide nanoparticles in Mo(VI)/FeNPs-based biosensors","authors":"Kaouthar Ahmouda ,&nbsp;Mohammed Tayeb Oucif Khaled","doi":"10.1016/j.plaphy.2025.109723","DOIUrl":"10.1016/j.plaphy.2025.109723","url":null,"abstract":"&lt;div&gt;&lt;div&gt;The goal of this study is to investigate how the green synthesis influences the crystallinity, stability, and catalytic performance of green-synthesized iron oxide nanoparticles (FeNPs) in Mo(VI)/FeNPs-based biosensors for ascorbic acid (AA) colorimetric detection. By examining the correlation between total antioxydant capacity (TAC) and FeNPs' structural properties, phase composition, and defect levels, the study aims to establish how plant-mediated synthesis drives FeNPs' catalytic efficiency, ultimately enhancing biosensor sensitivity and lowering detection limits (LOD and LOQ). Statistical analyses, including ANOVA and Pearson correlation, are applied to validate the relationship between TAC and FeNPs' characteristics, reinforcing the role of green synthesis in enhancing biosensor performance. In this study, FTIR spectroscopy was employed to analyze unoxidized free AA groups, offering detailed insights into oxidation preferences across various Mo(VI)/FeNPs pairs. The results showed that AA was preferentially oxidized at the four biosensors with a consistent oxidation peak at 820 nm across all Mo(VI)/FeNPs pairs, with a linear correlation to AA concentrations from 0.05 to 100 mM. FTIR analysis of unoxidized &lt;span&gt;AA&lt;/span&gt; supported these findings, revealing that &lt;span&gt;AA&lt;/span&gt; oxidation was most efficient at Mo(VI)/ROS-FeNPs and Mo(VI)/ARM-FeNPs biosensors compared to Mo(VI)/JUN-FeNPs and Mo(VI)/MAT-FeNPs. Likewise, the highest sensitivity, reflected by the lowest LOD (0.01183 ± 0.00116 mM and 0.01521 ± 0.00187) and LOQ (0.0393 ± 0.00386 mM and 0.0506 ± 0.00623), was observed in Mo(VI)/ROS-FeNPs and Mo(VI)/ARM-FeNPs, whereas Mo(VI)/JUN-FeNPs and Mo(VI)/MAT-FeNPs exhibited higher LOD (0.03237 ± 0.00318 mM and 0.03550 ± 0.00348) and LOQ (0.107887 ± 0.01058 mM and 0.11834 ± 0.01159), confirming the impact of FeNPs' catalytic performance on detection sensitivity. One-way ANOVA analysis confirmed that these variations in LOD (F = 42.7, &lt;em&gt;p&lt;/em&gt; &lt; 0.0001) and LOQ (F = 58.3, &lt;em&gt;p&lt;/em&gt; &lt; 0.0001) were statistically significant (&lt;em&gt;p&lt;/em&gt; &lt; 0.05), indicating that intrinsic properties of FeNPs strongly influence the catalytic performance of the biosensors. Post-hoc Tukey's test revealed that FeNPs synthesized with extracts of higher TAC, such as Rosmarinus officinalis and Artemisia herba-alba, achieved significantly lower LOD and LOQ values compared to those prepared with Juniperus phoenicia and Matricaria pubescens extracts, signifying superior catalytic performance. The catalytic performances of FeNPs in AA oxidation are closely linked to their stability and crystallinity. XRD analysis revealed that higher-TAC extracts, like Rosmarinus officinalis and Artemisia herba-alba, yielded FeNPs with minor defects, with a greater percentage of the &lt;em&gt;γ&lt;/em&gt; − &lt;em&gt;Fe&lt;/em&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;em&gt;O&lt;/em&gt;&lt;sub&gt;3&lt;/sub&gt; phase, indicating enhanced stability and crystallinity. In contrast, extracts with lower TAC, such as Juniperus phoenicia and Matrica","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109723"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of copper-containing nanoparticles and their influence on plant growth and development.","authors":"A I Perfileva, B G Sukhov, T V Kon'kova, E I Strekalovskaya, K V Krutovsky","doi":"10.1016/j.plaphy.2025.109575","DOIUrl":"10.1016/j.plaphy.2025.109575","url":null,"abstract":"<p><p>Copper (Cu) is an important microelement for plants, but in high concentrations it can be toxic. Cu-containing nanoparticles (Cu NPs) are less toxic, their use for plants is safer, more effective and economical than the use of Cu salts. This review presents detailed information on the chemical diversity of Cu NPs and various methods of their synthesis. The mechanisms of the effect of Cu NPs on plants are described in detail, and examples of research in this area are given. The main effects of Cu NPs on plants are reviewed including on their growth and development (organogenesis, mitosis, accumulation of biomass), biochemical processes (intensity of photosynthesis, antioxidant status and intensity of lipid peroxidation processes), gene expression, plant resistance to abiotic and biotic stress factors. The prospects of using Cu NPs as mineral fertilizers are shown by describing their stimulation effects on seed germination, plant growth and development, and on increase of plant resistance to stress factors. The protective effect of Cu NPs is often explained by their antioxidant activity. At the same time, there are a number of studies demonstrating the negative impact of Cu NPs on plant growth, development and the intensity of photosynthesis, depending on their concentration. Cu NPs have a pronounced antibacterial effect on bacterial phytopathogens of cultivated plants, as well as on a number of phytopathogenic fungi and nematodes. Thus, Cu NPs are promising agents for agriculture, while their effect on plants requires careful selection of optimal concentrations and comprehensive studies to avoid a toxic effect.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109575"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function of key ion channels in abiotic stresses and stomatal dynamics.","authors":"Yuanyuan Zuo, Asad Abbas, Seidat Oluwadamilola Dauda, Chen Chen, Jayakumar Bose, Michelle Donovan-Mak, Yuanyuan Wang, Jing He, Peng Zhang, Zehong Yan, Zhong-Hua Chen","doi":"10.1016/j.plaphy.2025.109574","DOIUrl":"10.1016/j.plaphy.2025.109574","url":null,"abstract":"<p><p>Climate changes disrupt environmental and soil conditions that affect ionic balance in plants, presenting significant challenges to their survival and productivity. Membrane transporters are crucial for maintaining ionic homeostasis and regulating the movement of substances across plasma and organellar membranes, particularly under abiotic stresses. Among these abiotic stress-responsive mechanisms, stomata are critical for regulating water loss and carbon dioxide uptake, reflecting a plant's ability to respond and adapt to abiotic stresses effectively. This review highlights the role of ion transporters, including both anion and cation transporters in plant abiotic stress responses. It explores the interplay between different ion channels and regulatory components that enable plants to withstand key abiotic stresses such as drought, salinity, and heat. Moreover, we emphasized the contributions of three essential types of ion channels - potassium, anion, and calcium to abiotic stress-related stomatal regulation. These ion channels orchestrate complex signaling networks that allow plants to modulate stomatal behavior and maintain physiological balance under adverse conditions. This article provides valuable molecular and physiological insights into the mechanisms of ion transport and regulation for plants to adapt to environmental challenges. Thus, this review offers a useful foundation for developing innovative strategies to enhance crop resilience and performance in an era of increasingly unpredictable and harsh climates.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109574"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waterlogging stress mechanism and membrane transporters in soybean (Glycine max (L.) Merr.).","authors":"Ambika Rajendran, Ayyagari Ramlal, Amooru Harika, Sreeramanan Subramaniam, Dhandapani Raju, S K Lal","doi":"10.1016/j.plaphy.2025.109579","DOIUrl":"10.1016/j.plaphy.2025.109579","url":null,"abstract":"<p><p>An excess of water is more harmful to plant growth, root growth and the uniformity of the plant population than a water deficit. Water is a crucial factor in the three basic stages of soybean development: germination, emergence and flowering/seed filling. Waterlogging is one of the biggest constraints to crop production and productivity in India and can occur at any stage in soybean. However, seeds and seedlings are damaged by waterlogging resulting in a significant reduction in grain yield. Seed yield and growth are significantly correlated at the seedling stage. In addition, the plant is under constant pressure due to changing environmental conditions and has difficulty withstanding these harsh, unpredictable and difficult situations. Membrane transporters are essential and play fundamental roles during waterlogging thereby facilitating cellular homeostasis and gaseous exchange, which support plant growth and development. This review highlights the genetic basis and mechanism of waterlogging tolerance in soybean and the role of climate in influencing the genetic makeup of the crop, paving the way for further development of improved soybean varieties. Simultaneously, the article highlights membrane transporters' importance in water-mediated stress in soybeans.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109579"},"PeriodicalIF":6.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of transcriptome, proteome, and phosphoproteome reveals the complexity of early nitrogen responses in poplar roots","authors":"Ruhui Chang ,&nbsp;Fengbo Xue ,&nbsp;Zhaoyin Hou ,&nbsp;Hongye Guo ,&nbsp;Lina Cao ,&nbsp;Shuang Zhang ,&nbsp;Wenjie Wang ,&nbsp;Chunpu Qu ,&nbsp;Chuanping Yang ,&nbsp;Guanjun Liu ,&nbsp;Zhiru Xu","doi":"10.1016/j.plaphy.2025.109703","DOIUrl":"10.1016/j.plaphy.2025.109703","url":null,"abstract":"<div><div>Nitrogen (N) availability is a key factor in plant growth, but the molecular mechanisms underlying the early responses of poplar (<em>Populus</em> × <em>xiaohei</em> T. S. Hwang &amp; Liang) roots to nitrogen are not well understood. The primary objective of this study was to elucidate these early molecular responses by integrating transcriptome, proteome, and phosphoproteome under low-nitrogen (LN, 0.2 mM NH₄NO₃) and high-nitrogen (HN, 2 mM NH₄NO₃) conditions. Specifically, the objectives of this study were: (i) to identify key metabolic pathways involved in nitrogen responses in poplar roots; (ii) to explore the relationship between differentially expressed genes (DEGs) and transcription factors (TFs) within these pathways; and (iii) to construct co-expression networks to uncover the regulatory mechanisms of nitrogen signaling. KEGG pathway enrichment analysis indicated that nitrogen metabolism and phenylpropanoid metabolism were key pathways in RNA-seq and proteome, while starch and sucrose metabolism were crucial in transcriptome and phosphoproteome. Plant hormone signal transduction was a key pathway in transcriptome, and gluconeogenesis/glycolysis was essential in proteome. WGCNA revealed three key modules (MEgreenyellow, MEblack, and MEblue) significantly associated with physiological indices, including NO₃⁻, soluble sugar, and sucrose contents. Co-expression networks highlighted TFs as central regulators of nitrogen-responsive pathways, with distinct expression patterns between LN and HN treatments. These findings elucidate the complexity of nitrogen-regulated metabolic networks in poplar roots and reveal potential links between nitrogen signaling, carbohydrate metabolism, and secondary metabolism. This study provides a foundation for improving nitrogen-use efficiency in forest trees, with implications for sustainable forestry and ecosystem management.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109703"},"PeriodicalIF":6.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutional, expressional and functional analysis of WRKY gene family reveals that PbeWRKY16 and PbeWRKY31 contribute to the Valsa canker resistance in Pyrus betulifolia","authors":"Chenglong Du ,&nbsp;Hongqiang Yu ,&nbsp;Huanhuan Hu ,&nbsp;E. Sun ,&nbsp;Minrui Cai ,&nbsp;Zhiqi Dou ,&nbsp;Han Dong ,&nbsp;Cunwu Zuo","doi":"10.1016/j.plaphy.2025.109719","DOIUrl":"10.1016/j.plaphy.2025.109719","url":null,"abstract":"<div><div>The WRKY transcription factor family plays a crucial role in regulating plant growth and stress responses. However, there are few studies on the regulation of resistance to Valsa canker. In this study, a comprehensive analysis of WRKY genes across 19 plant species was conducted. The potential members of Valsa canker resistance regulation were identified via functional validation. A total of 1641 WRKY genes could be categorized into seven groups. WRKY family members show subfamily- and species-specific expansions. In Rosaceae, Group II-d and II-e were rapidly expanded, which mainly originated based from whole genome duplication (WGD). Cis-element analysis and protein interaction network prediction underscored that most WRKYs respond to stress signals. Based on expressional investigation and Weighted Gene Co-expression Network Analysis (WGCNA), 9 WRKY genes in <em>Pyrus betulaefolia</em> were screened as candidates for Valsa canker resistance regulation. Functional analysis further demonstrated that PbeWRKY16 and PbeWRKY31 regulate the expression of genes involved in salicylic acid (SA) biosynthesis and transport, thereby enhancing resistance to Valsa canker and activating immune responses. Our results provide a foundation for understanding the evolutionary mechanisms of the WRKY gene family and screened potential family members on Valsa canker resistance regulation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109719"},"PeriodicalIF":6.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulation of UV-B - Triggered ABA signal on isoflavones synthesis in soybean suspension cells","authors":"Mian Wang ,&nbsp;Yiting Wang ,&nbsp;Chong Xie ,&nbsp;Pei Wang ,&nbsp;Runqiang Yang","doi":"10.1016/j.plaphy.2025.109728","DOIUrl":"10.1016/j.plaphy.2025.109728","url":null,"abstract":"<div><div>Isoflavones are abundant antioxidant components in soybeans. UV-B radiation can induce the biosynthesis of isoflavones in soybean suspension cells, but the underlying molecular mechanism remains unclear. The transcriptome analysis revealed that UV-B radiation influenced the biosynthesis and signal transduction of the phytohormone abscisic acid (ABA) during the biosynthesis of isoflavones in soybean suspension cells. This suggests that ABA may be a key mediator of isoflavones synthesis in soybean suspension cells exposed to UV-B. Therefore, this study investigated the critical role of ABA on isoflavones synthesis in soybean suspension cells exposed to UV-B. The content of endogenous ABA increased significantly by 97% in suspension cells exposed to UV-B. Moreover, the application of exogenous ABA led to a rise in intracellular ABA levels. The elevated intracellular ABA triggered the ABA receptor PYL/PYR, thereby initiating ABA signal transduction. This activation, in turn, up-regulated the expression of seven key enzymes involved in isoflavones synthesis, including phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). Consequently, there was a significant increase of 73% and 49% in the total isoflavones content in UV-B and ABA groups, respectively. Additionally, the ABA synthesis inhibitor fluridone suppressed the synthesis of isoflavones in suspension cells. These findings collectively highlight the pivotal role of ABA, a plant signaling molecule, in response to UV-B radiation, culminating in the enhanced accumulation of isoflavones in soybean suspension cells.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109728"},"PeriodicalIF":6.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 1R-MYB transcription factor SlMYB1L modulates drought tolerance via an ABA-dependent pathway in tomato","authors":"Zhouyuan Liu,&nbsp;Jianan Li,&nbsp;Shuang Li,&nbsp;Qianqian Song,&nbsp;Min Miao,&nbsp;Tingting Fan,&nbsp;Xiaofeng Tang","doi":"10.1016/j.plaphy.2025.109721","DOIUrl":"10.1016/j.plaphy.2025.109721","url":null,"abstract":"<div><div>The MYB transcription factor family is one of the biggest transcription factors in plants, playing key roles in regulating many biological processes, including growth and development, responses to biotic and abiotic stresses and hormone signaling. In this study, we identified and characterized an 1R-MYB transcription factor, <em>SlMYB1L</em>, which is involved in regulating drought tolerance in tomato. <em>SlMYB1L</em>-RNAi transgenic plants displayed more severe dehydration phenotype with elevated malondiadehyde (MDA) and hydrogen peroxide (H₂O₂), as well as reduced proline content and antioxidant enzyme activities compared to wild-type under drought stress. Additionally, <em>SlMYB1L</em> influenced drought-induced stomatal closure and modulated endogenous ABA levels, leading to a decrease in the expression of ABA-related genes in <em>SlMYB1L</em>-RNAi transgenic plants. A dual-luciferase reporter assay further confirmed that <em>SlMYB1L</em> represses the expression of ABA catabolism gene <em>SlCYP707A3</em>. In conclusion, our findings suggest that <em>SlMYB1L</em> is a stress-responsive transcription factor that positively regulates drought tolerance and may serve as a candidate gene for developing drought-resistant crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109721"},"PeriodicalIF":6.1,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}