Plant Physiology and Biochemistry最新文献

{"title":"Exogenous application of melatonin and chitosan mitigate simulated microgravity stress in the Rocket (Eruca sativa L.) plant","authors":"Hilda Amiripour ,&nbsp;Alireza Iranbakhsh ,&nbsp;Sara Saadatmand ,&nbsp;Fateme Mousavi ,&nbsp;Zahra Oraghi Ardebili","doi":"10.1016/j.plaphy.2024.109294","DOIUrl":"10.1016/j.plaphy.2024.109294","url":null,"abstract":"<div><div>Starting life in space and implementing spaceflight missions requires raising of plants in special conditions, where various stresses, including microgravity, are applied to plant. The use of stimulants is known as a promising effective approach that enhances plant resistance encountered a variety of abiotic stresses. In this study, the impact of two stimulants, melatonin and chitosan, in reducing negative effects of clinorotation on Rocket (<em>Eruca sativa</em> L.) seedlings was investigated from a physiological and biochemical point of view. For this purpose, a completely randomized experiment was designed where the treatments included control (without stimulants and normal gravity), melatonin (100 μM), chitosan (230 M), microgravity, microgravity + melatonin, and microgravity + chitosan. The results disclosed that the microgravity significantly impaired the plant growth and morphology, while exogenous application of melatonin and chitosan improved the plant growth parameters under stress conditions. Under microgravity, there was a reduction of 46.15% in shoot length (4.9 mm) and 41.44% in root length (4.7 mm) compared with the control (9.1 mm; 8.03 mm), respectively. Clinorotation led to a marked increment in the enzymes activity, wherein the POD, SOD and CAT activities increased by 75.13%, 72.67%, and 53.42%, respectively, compared with the control seedlings. In addition, supply of these two stimulants strengthened the scavenging of radial oxygen species and helped the plant to tolerate stress conditions, by activated the enzymatic and non-enzymatic systems. These results can pave the road for more studies and broad application of biological stimuli to overcome the space harsh environmental conditions by plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"218 ","pages":"Article 109294"},"PeriodicalIF":6.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of pyrabactin resistance 1-like (PYL) family genes revealed AhPYL6 confer the resistance to Ralstonia solanacearum in peanut","authors":"Zenghui Cao ,&nbsp;Zhan Li ,&nbsp;Lin Meng,&nbsp;Di Cao,&nbsp;Kai Zhao,&nbsp;Sasa Hu,&nbsp;Yanzhe Li,&nbsp;Kunkun Zhao,&nbsp;Qian Ma,&nbsp;Yaoyao Li,&nbsp;Yi Fan,&nbsp;Xingli Ma,&nbsp;Fangping Gong,&nbsp;Zhongfeng Li,&nbsp;Ding Qiu,&nbsp;Lin Zhang,&nbsp;Xingguo Zhang,&nbsp;Rui Ren,&nbsp;Dongmei Yin","doi":"10.1016/j.plaphy.2024.109295","DOIUrl":"10.1016/j.plaphy.2024.109295","url":null,"abstract":"<div><div>Bacterial wilt (BW) caused by <em>Ralstonia solanacearum</em> severely impacts the yield and quality of peanut (<em>Arachis hypogaea</em> L.), a globally cultivated industrial crop. Despite the abscisic acid (ABA) signaling pathway have been identified as key factors in peanut resistance to BW, the molecular mechanism remains unclear. Through systematic identification, it was discovered that the peanut genome contains 18 ABA receptor pyrabactin resistance 1-like (PYL) family genes, which show conservation with other plant species. Among these <em>PYL</em> genes in peanut (referred to as <em>AhPYL</em>), <em>AhPYL6</em> and <em>AhPYL16</em> showed significant up-regulation in response to salicylic acid, jasmonic acid, ABA treatments, and <em>R. solanacearum</em> infection. Subsequently, the full-length <em>AhPYL6</em> was cloned and functionally characterized. The fusion protein AhPYL6-YFP was predominantly expressed in the cytoplasm and nucleus of tobacco leaves, and overexpression of <em>AhPYL6</em> notably enhanced resistance against <em>R. solanacearum</em>. Expression analysis revealed that the expression levels defense -related genes including <em>NbNPR1</em>, <em>NbPR2</em>, <em>NbPR3</em>, <em>NbHRS203</em>, <em>NbEFE26</em>, and <em>NbNDR1</em> were significantly up-regulated by the overexpression of <em>AhPYL6</em>, which suggested that <em>AhPYL6</em> confers the resistance to <em>R. solanacearum</em> through promoting expression of defense -related genes. These findings highlight the potential roles of PYL ABA receptors in the plant defense response to bacterial pathogens.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109295"},"PeriodicalIF":6.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MaMPK19, a key gene enhancing cold resistance by activating the CBF pathway in banana","authors":"Fan Zhengyang ,&nbsp;Zhao Bianbian ,&nbsp;Zeng Yuhan ,&nbsp;Lai Ruilian ,&nbsp;Zhao Xiaobing ,&nbsp;Chen Yukun ,&nbsp;Lin Yuling ,&nbsp;Du Yinggang ,&nbsp;Lai Zhongxiong","doi":"10.1016/j.plaphy.2024.109290","DOIUrl":"10.1016/j.plaphy.2024.109290","url":null,"abstract":"<div><div><em>MPKs</em> play an essential part role in the process of plant low temperature stress. In this study, the specific inhibitor SB203580 of <em>MPK</em> was used to spray banana leaves and <em>MaMPK19</em> was overexpressed in <em>N.benthamiana</em> and banana to explore the effect of <em>MaMPK19</em> on cold resistance and the regulation mode of downstream genes. Additionally, we optimized the method of genetic transformation of banana laying the foundation for the establishment of an efficient genetic transformation system. The results showed that 40 μmol L⁻¹ SB203580 could significantly reduce the expression of <em>MaMPK19</em> and <em>MaCBFs</em>, as well as weaken the cold resistance of banana at 4 °C. After <em>agrobacterium tumefaciens</em> infection, the regeneration rates of adventitious buds in ‘Tianbao’, ‘Brazilian’ and‘Indonesia’ (<em>Musa</em> spp. AAA Group, Cavendish) reached 10.43%, 15.81% and 14.23%, respectively. And the positive rates reached 10.71%, 2.25% and 6.94%, respectively. Overexpression of <em>MaMPK19</em> enhanced the cold resistance of <em>N.benthamiana</em> and bananas. <em>MaMPK19</em> promoted the expression of <em>MaICE1</em>, <em>MaDREB1D</em> and <em>MaCOR413</em>. Furthermore, <em>MaMPK19</em> increased POD activity and the content of ABA and JA. Our study highlights the importance of <em>MaMPK19</em> in improving the cold resistance of bananas and provides a reference for biological breeding.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109290"},"PeriodicalIF":6.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of ferric reductase oxidase (FRO) genes in Gossypium spp. reveal their crucial role in iron homeostasis under abiotic and biotic stress","authors":"Kavita Nanda ,&nbsp;Maninder Singh ,&nbsp;Tikshana Yadav ,&nbsp;Vipin Kumar Tiwari ,&nbsp;Varsha Singh ,&nbsp;Vijay Pratap Singh ,&nbsp;Samir V. Sawant ,&nbsp;Surendra Pratap Singh","doi":"10.1016/j.plaphy.2024.109281","DOIUrl":"10.1016/j.plaphy.2024.109281","url":null,"abstract":"<div><div>Ferric Reductase Oxidase (FRO) genes are pivotal in iron uptake and homeostasis in plants, yet they are not studied in cotton. Here, we identify and analyze 65 FRO homologs (21 <em>GhFRO</em>, 21 <em>GbFRO</em>, 11 <em>GaFRO</em>, 12 <em>GrFRO</em>) across four <em>Gossypium</em> species (<em>G. hirsutum</em>, <em>G. barbadense</em>, <em>G. arboreum</em>, <em>G. raimondii</em>). FRO exhibit conserved ferric reductase activity and conserved domain structures; Ferric_reduct (PF01794), FAD_binding_8 (PF08022), and NAD_binding_6 (PF08030) across species. Physicochemical properties and subcellular localization analysis provided insights into FRO proteins' functional characteristics, mainly localized to the plasma membrane. Phylogenetic analysis delineates 11 groups, indicating both conserved and divergent evolutionary patterns. Gene structure analysis unveils varying exon-intron compositions. Chromosomal localization shows distribution across A and D genomes, suggesting evolutionary dynamics. Synteny analysis reveals paralogous and orthologous gene pairs subjected to purifying selection. The cis-regulatory elements analysis implicates diverse regulatory mechanisms. Expression profiling highlights dynamic regulation across developmental stages, abiotic and biotic stress conditions. GhFRO interacts with Ca⁺⁺-dependent protein kinases-10/28-like (CDPKs10/28-like) and metal transporter Natural resistance-associated macrophage protein 6 (Nramp6) to regulate metal ion transport and iron homeostasis. The three-dimensional protein structure prediction suggests potential ligand-binding sites in FRO proteins. Moreover, qRT-PCR analysis of selected eight <em>GhFROs</em> in leaves treated with stress elicitors, MeJA, SA, NaCl, and PEG for 1h, 2h, 4h, and 6h revealed significant downregulation. Overall, this comprehensive study provides insights into FRO gene diversity, evolution, structure, regulation, and function in cotton, with implications for understanding plant iron homeostasis and stress responses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109281"},"PeriodicalIF":6.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and molecular evidence of HvMORF8 conferring drought-tolerance in barley","authors":"Shou-Heng Shi ,&nbsp;Muhammad Zeeshan ,&nbsp;Wu-Nian Shan ,&nbsp;Cheng-Wei Qiu ,&nbsp;Zhong-Hua Chen ,&nbsp;Feibo Wu","doi":"10.1016/j.plaphy.2024.109289","DOIUrl":"10.1016/j.plaphy.2024.109289","url":null,"abstract":"<div><div>Drought is one of the most devastating abiotic stresses worldwide, which severely limits crop yield. Tibetan wild barley is a treasure trove of useful genes for crop improvement including drought tolerance. Here, we detected large-scale changes of gene expression in response to drought stress with a substantial difference among contrasting Tibetan barley genotypes XZ5 (drought-tolerant), XZ54 (drought-sensitive) and <em>cv</em>. Tadmor (drought-tolerant). Drought stress led to upregulations of 142 genes involved in transcription, metabolism, protein synthesis, stress defense, transport and signal transduction in XZ5, but those genes were down-regulated or unchanged in XZ54 and Tadmor. We identified and functionally characterized a novel <em>multiple organellar RNA editing factors 8</em> (<em>HvMORF8</em>), which was up-regulated by drought stress in XZ5, but unchanged in XZ54 and Tadmor under drought stress. Phylogenetic analysis showed that orthologues of HvMORF8 can be traced back to the closest gymnosperm species such as <em>Cycas micholitzii</em>, implicating a potential evolutionary origin for MORF8 from a common ancestor in early seed plants. Virus-induced <em>HvMORF8</em> silencing in XZ5 led to hypersensitivity to drought stress, demonstrating it is a positive regulator of drought tolerance in barley. RNA sequencing of BSMV:HvMORF8 and control plants reveals that silencing of <em>HvMORF8</em> suppresses genes involved in osmolytes transport, cell wall modification and antioxidants, resulting in water metabolism disorder and overaccumulation of reactive oxygen species (ROS) under drought stress. Therefore, we propose <em>HvMORF8</em>-mediated regulatory drought tolerance mechanisms at transcriptomic level in XZ5, providing new insight into the genetic basis of plastid RNA editing function of <em>HvMORF8</em> for barley drought tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109289"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of phenylpropanoid pathway in genetic regulation and physiological adaptation in arsenic stressed rice genotypes","authors":"Himanshu Saini ,&nbsp;Medha Panthri ,&nbsp;Priyanka Bhatia ,&nbsp;Meetu Gupta","doi":"10.1016/j.plaphy.2024.109291","DOIUrl":"10.1016/j.plaphy.2024.109291","url":null,"abstract":"<div><div>This study investigates the role of the phenylpropanoid pathway in arsenic (As) contaminated rice genotypes under natural conditions, exploring the intricate relationship between genetic regulation and physiological adaptation. Differential approaches adapted by rice genotypes to counteract As exposure are elucidated here through analysis of enzyme activities and related gene expression patterns, docking simulations, and nutrient dynamics. Enzymatic analysis from the phenylpropanoid pathway revealed significant variations across rice genotypes, with Mini mansoori exhibiting notably higher activity levels of key enzymes (PAL, C4H, 4CL, CHI, DFR and F3H) compared to Sampoorna and Pioneer. Additionally, the gene expression profiling unveiled differential responses, with Mini mansoori and Pioneer demonstrating higher expression of genes (<em>OsPAL, OsCHS, OsCHI, OsF3H, OsF3′H, OsFLS, OsDFR</em>, and <em>OsLAR</em>) associated with As resistance and tolerance, compared to Sampoorna. Enrichment analysis emphasized the involvement of cinnamic acid biosynthesis and related pathways. Molecular docking depicted certain proteins, such as Os4CL, OsFLS, OsDFR, and OsLAR susceptible to As binding, potentially affecting enzymatic activity. Ionomic analysis unveiled that Mini mansoori maintained higher levels of essential nutrients such as Na, Ca, P, Mn, Mg, and Zn in grains. However, this contrasted with Pioneer and Sampoorna, which experienced nutrient imbalance likely due to higher As accumulation. Chlorophyll fluorescence analysis depicted that Mini mansoori and Pioneer maintained better photosynthetic efficiency under As toxicity compared to Sampoorna. Moreover, network analysis highlights the critical role of Mg and Na interaction with essential phenolics and flavonoids, in combating the stress. Harnessing this understanding, targeted breeding effort could yield As-resistant rice varieties with enhanced nutrient and flavonoid contents, addressing both food safety and malnutrition in affected regions.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109291"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced bacoside synthesis in Bacopa monnieri plants using seed exudates from Tamarindus indica","authors":"Titir Guha ,&nbsp;Ekta Bhattacharya ,&nbsp;Madhurima Dutta ,&nbsp;Anisha Dutta ,&nbsp;Moumita Dandapat ,&nbsp;Rahul Bose ,&nbsp;Suparna Mandal Biswas","doi":"10.1016/j.plaphy.2024.109287","DOIUrl":"10.1016/j.plaphy.2024.109287","url":null,"abstract":"<div><div>Diverse allelochemicals are released from different plant parts via leaching, exudation, volatilization, etc., which can induce either stimulatory or inhibitory effects depending on the target plant species. Very few reports provide details about allelopathic interaction through seed exudates. Since <em>Tamarindus indica</em> L. seed exudate (TSE) has been known to exhibit growth stimulatory effect on lettuce, radish, and sesame, in the present study we have evaluated its role in regulating the secondary metabolism of an over-exploited medicinal herb, <em>Bacopa monnieri</em> (L.) Pennel. The bacoside biosynthesis rate of <em>B. monnieri</em> is quite low in comparison to its increasingly high demands in the pharmaceutical industry. Currently, researches are aimed towards enhancing the biosynthesis of this secondary metabolite <em>in planta</em> by utilizing external stress factors. Presently, 7-day-old <em>B. monnieri</em> seedlings were treated with 1:16, 1:8, 1:4, 1:3, and 1:2 (seed weight: water) TSE. Maximum upregulation of secondary metabolite contents was found in the 1:4 (seed weight: water) TSE treatment set. This TSE treatment also enhanced H₂O₂ and salicylic acid production leading to the upregulation of the genes related to the MVA pathway (<em>BmAACT</em>, <em>BmHMGR</em>, <em>BmMDD</em>, <em>BmSQS, and BmBAS</em>) which are responsible for bacoside biosynthesis and 1.7-fold higher bacoside level was found in TSE treated set compared to control. LC-HRMS analysis of TSE confirmed the presence of alkaloid (lupanine), phenol (chlorogenic acid), and organic acid (mucic acid), which are identified as potential allelochemicals responsible for modulating the secondary metabolism of <em>B. monnieri</em>. Thus, this study highlights a sustainable approach towards enhancing bacoside production <em>in planta</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109287"},"PeriodicalIF":6.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do different wheat ploidy levels respond differently against stripe rust infection: Interplay between reactive oxygen species (ROS) and the antioxidant defense system?","authors":"Farkhandah Jan, Parthiban M, Satinder Kaur, Mohd Anwar Khan, Farooq Ahmad Sheikh, Fehim Jeelani Wani, A A Saad, Yogita Singh, Upendra Kumar, Vikas Gupta, Mahendar Thudi, Dinesh K Saini, Sundeep Kumar, Rajeev Kumar Varshney, Reyazul Rouf Mir","doi":"10.1016/j.plaphy.2024.109259","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109259","url":null,"abstract":"<p><p>Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most damaging wheat disease, causing substantial losses in global wheat production and productivity. Our study aimed to unravel the complex reciprocity between reactive oxygen species and the antioxidant defense system as a source of resistance against stripe rust in diploid, tetraploid and hexaploid wheat genotypes. The significant genetic variability for stripe rust in the materials under study was evident as the genotypes showed contrasting responses during both the adult and seedling stages. Our thorough perspective on the biochemical responses of wheat genotypes to stripe rust infection revealed distinct patterns in oxidative damage, antioxidant enzymes and photosynthetic pigments. Principal component analysis revealed inverse correlations between antioxidants and ROS, underscoring their key function in maintaining the cellular redox balance and protecting plants against oxidative damage. Diploid (Ae. tauschii) wild wheat exhibited a better biochemical defense system and greater resistance to stripe rust than the tetraploid (T. durum) and hexaploid (Triticum aestivum) wheat genotypes. The antioxidant enzyme activity of durum wheat was moderate compared to diploid and hexaploid wheat genotypes. The hexaploid wheat genotypes exhibited increased ROS production, reduced antioxidant enzyme activity and decreased photosynthetic pigment levels. This study enhances understanding of the antioxidant defense system across different wheat ploidies facing stripe rust, serving as a valuable strategy for improving crop disease resistance. This study validated the biochemical response of stripe rust-resistant and susceptible candidate genotypes, which will be used to develop genetic resources for discovering stripe rust resistance genes in wheat.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109259"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated quality, physiological and transcriptomic analysis reveals mechanisms of kiwifruit response to postharvest transport vibrational stress","authors":"Chenxu Zhao ,&nbsp;Linlin Cheng ,&nbsp;Yurong Guo ,&nbsp;Wei Hui ,&nbsp;Junpeng Niu ,&nbsp;Shujie Song","doi":"10.1016/j.plaphy.2024.109285","DOIUrl":"10.1016/j.plaphy.2024.109285","url":null,"abstract":"<div><div>The ‘Xuxiang’ kiwifruit, a leading cultivar in China known for its high quality and yield, experiences quality degradation due to vibration stress during postharvest transportation. This study simulated the postharvest transportation vibrations of ‘Xuxiang’ kiwifruits to investigate the effects on the fruit quality and physiology. Different vibration intensities (0.26, 0.79, and 1.5 m s⁻²) and durations (0, 24, 48, 72, and 96 h) were applied to analyze the quality, physiological and transcriptomic changes of fruits after vibration stress, as well as the association between quality deterioration, gene networks, and key genes. Results indicated that vibration stress significantly accelerated the deterioration of fruit quality and induced physiological changes. As vibration intensity and duration increased, there was a rapid decrease in fruit firmness and an increase in weight loss, soluble solid content, relative conductivity, ethylene production, respiratory rate, and malondialdehyde levels. The most severe deterioration in fruit quality occurred at a vibration intensity of 1.5 m s⁻². Transcriptome sequencing analysis was conducted on samples from different durations of exposure to the 1.5 m s⁻² vibration intensity. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses identified key genes associated with ethylene metabolism and softening. Weighted Gene Co-Expression Network Analysis (WGCNA) and correlation analysis further determined that 24 of these genes were regulated by vibrational stress, impacting ethylene metabolism and cell wall degradation. Vibration stress induced changes in genes related to ethylene metabolism and cell wall degradation, promoting lipid peroxidation and respiratory changes, which compromise cell membrane integrity and lead to quality deterioration. Compared with untreated fruits, vibration stress caused the quality deterioration, physiological changes and transcriptional regulation of kiwifruits, indicating that kiwifruits respond to vibration stress through multiple aspects. It proposes a fresh outlook on the understanding of the mechanism of transport vibration stress and further illustrates the importance of monitoring vibration intensity and duration as well as reducing vibration.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109285"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB-1 regulates anthocyanin biosynthesis in Magnolia wufengensis","authors":"Xian-ping Liu ,&nbsp;Min Luo ,&nbsp;Xiu-qi Liu ,&nbsp;Liu-yan Hao ,&nbsp;Chen Zhu ,&nbsp;Li Wang ,&nbsp;Lv-yi Ma","doi":"10.1016/j.plaphy.2024.109283","DOIUrl":"10.1016/j.plaphy.2024.109283","url":null,"abstract":"<div><div>Anthocyanin is an essential pigment in all major horticultural crops especially in ornamental trees. <em>Magnolia wufengensis</em> (new species of <em>Magnolia</em>) with red color flower was recently found as a popular species for ornamental use, but anthocyanin synthesis and regulation in <em>M</em>. <em>wufengensis</em> are poorly understood. Herein, transcriptome analysis was used to decipher the gene network associated with anthocyanin biosynthesis. An R2R3-like MwMYB-1 transcription factor was found. MwMYB-1 overexpression resulted in anthocyanin accumulation in tobacco and Arabidopsis. MwMYB-1 worked independently rather than forming a protein complex with bHLH or WD40 protein. According to MwMYB-1 DAP-seq analysis in Arabidopsis, the MwMYB-1 transcription factor preferred to bind the “AAGAGAG” motif (DREME-5) in the third exon of the <em>AtMYB75</em> gene. The yeast one hybrid assay and transcription activity assay further confirmed this. Thus, MwMYB-1 activated <em>AtMYB75</em> gene expression and conducted cascade amplification of anthocyanin biosynthesis. Taken together, our findings provide a novel understanding of anthocyanin biosynthesis regulation in <em>M. wufengensis</em> and can be used to promote agronomic trait improvement in tree species.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109283"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}