Plant Physiology and Biochemistry最新文献

{"title":"Comprehensive analysis of sweet cherry UDP-glycosyltransferases and functional validation of PavUGT10 for improving submergence tolerance","authors":"Muhammad Usman ,&nbsp;Muhammad Aamir Manzoor ,&nbsp;Li Wang ,&nbsp;Wanxia Sun ,&nbsp;Xiaojuan An ,&nbsp;Zixing Sun ,&nbsp;Fei Yu ,&nbsp;Ruie Liu ,&nbsp;Caixi Zhang","doi":"10.1016/j.plaphy.2025.110503","DOIUrl":"10.1016/j.plaphy.2025.110503","url":null,"abstract":"<div><div>Sweet cherry (<em>Prunus avium</em> L.) is an economically significant fruit crop, and improving its resilience to abiotic stress is crucial for sustainable production and industrial value. UDP-glycosyltransferases (UGTs) modulate hormone homeostasis, secondary metabolism, and stress responses in plants, yet their roles in sweet cherry remain largely uncharacterized. Here, we report a comprehensive genome-wide analysis of the PavUGT family, identifying 235 genes unevenly distributed across eight chromosomes and classified into 18 phylogenetic subfamilies. Promoter analysis revealed enrichment of stress-responsive cis-elements, and comparative synteny with multiple dicots including <em>Arabidopsis</em>, strawberry, apple, peach, and pear highlighted both conserved genomic blocks and lineage-specific expansions. Transcriptome profiling under waterlogging stress revealed a large set of PavUGTs with significant induction; twelve representative genes were selected for RT-qPCR validation, and <em>PavUGT10</em> was further characterized functionally. Subcellular localization of PavUGT10–GFP in <em>Nicotiana</em> leaves confirmed nuclear localization, consistent with predictions. Overexpression (OE) lines of <em>PavUGT10</em> in <em>Arabidopsis</em> significantly enhanced tolerance to 60 h of dark submergence followed by fifteen days of recovery; OE3 and OE7 achieved survival rates of 70 % and 75 %, respectively, compared to 25 % in WT. This enhanced tolerance was associated with elevated antioxidant enzyme activities (SOD, POD, CAT), increased proline accumulation, reduced MDA content, and reduced ROS accumulation, collectively indicating improved ROS scavenging and stress adaptation. These findings establish the <em>PavUGT</em> gene family as key players in cherry rootstocks’ adaptive responses to waterlogging and identify <em>PavUGT10</em> as a promising candidate for molecular breeding of flood-tolerant cultivars.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110503"},"PeriodicalIF":5.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into metabolic profile and redox adjustment during ammonium-induced salt acclimation in sorghum plants","authors":"Stelamaris de Oliveira Paula-Marinho ,&nbsp;Rafael de Souza Miranda ,&nbsp;Gyedre dos Santos Araújo ,&nbsp;Isabelle Mary Costa Pereira ,&nbsp;Rosilene Oliveira Mesquita ,&nbsp;Sergimar Kennedy de Paiva Pinheiro ,&nbsp;Emílio de Castro Miguel ,&nbsp;Lineker de Sousa Lopes ,&nbsp;Humberto Henrique de Carvalho ,&nbsp;Enéas Gomes-Filho","doi":"10.1016/j.plaphy.2025.110502","DOIUrl":"10.1016/j.plaphy.2025.110502","url":null,"abstract":"<div><div>The role of external nitrogen source (NO₃⁻ or NH₄⁺) against salinity-promoted damage on photosynthetic machinery and primary metabolism was investigated in <em>Sorghum bicolor</em> L. Sorghum growth was severely decreased by salinity, but the damage was less pronounced in NH₄⁺-fed plants. Closely, NH₄⁺ nutrition promoted better CO₂ uptake rate, associated with higher phosphoenolpyruvate carboxylase activity and maintenance of photosystem II efficiency, as well as better ionic regulation in comparison to NO₃⁻ nutrition. In parallel, although NH₄⁺ nutrition induced high basal H₂O₂ content, minor damage to chloroplast integrity was observed compared to NO₃⁻ after saline stress. In non-saline conditions, NH₄⁺-fed plants exhibited more connected network than NO₃⁻ nutrition, which led to decreased salt impact in network parameters after salt stress. This may be related to previous changes during acclimatization to NH₄⁺, allowing quick responses to secondary stresses, such as salinity. A metabolite set was significantly modulated by N source under salinity, including amino acids, sugar, and organic acids metabolism that displayed important contribution in response to salt stress. The asparagine amino acid was considered a key metabolite in alleviating NH₄⁺ toxicity. Despite the unchanged antioxidant enzymes system, NH₄⁺ nutrition increased the content of ascorbic acid, which may contribute to redox homeostasis and protect the chloroplasts against oxidative damage under salinity. Therefore, NH₄⁺ nutrition was able to activate mechanisms involved in photosynthetic efficiency and regulation of important metabolites, which attenuated the deleterious effects of salinity on sorghum plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110502"},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and physiological responses of wheat plants to polyphosphate and orthophosphate fertilizers supply under salt stress","authors":"Aicha Loudari ,&nbsp;Houssameddine Mansouri ,&nbsp;Gilles Colinet ,&nbsp;Abdallah Oukarroum","doi":"10.1016/j.plaphy.2025.110509","DOIUrl":"10.1016/j.plaphy.2025.110509","url":null,"abstract":"<div><div>Phosphorus (P) availability is significantly reduced under salt-stress conditions. Appropriate P fertilization can effectively alleviate its negative impact on plant growth and development. This study examines the impact of two P fertilizer forms (Ortho-B and Poly-B) on durum wheat tolerance to salinity (S1 = 3 dS m⁻¹ and S2 = 6 dS m⁻¹) under hydroponic conditions. A completely randomized design with six treatments and five replicates per treatment was used. At S2 salinity level, Poly-B outperformed Ortho-B (p &lt; 0.05) by increasing shoot and root dry weights by 867 % and 127 %, respectively. It also improved root morphology (length, surface area, and crossings) by up to 353 %, enhanced chlorophyll content by 41 %, and raised shoot phosphorus content by 20 %, indicating superior stress resilience. These findings suggest that polyphosphate fertilizers are more effective in mitigating salt stress effects in wheat. Future long-term field studies are recommended to confirm these results and assess their agronomic applicability at scale.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110509"},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible regulation of photosynthesis to low temperature in alfalfa contributed to spring rejuvenation","authors":"Meijun Liu ,&nbsp;Zheng Zhang ,&nbsp;An Yan ,&nbsp;Yuxiang Wang ,&nbsp;Miao Yu ,&nbsp;Wenjing Zhao","doi":"10.1016/j.plaphy.2025.110501","DOIUrl":"10.1016/j.plaphy.2025.110501","url":null,"abstract":"<div><div>Alfalfa (<em>Medicago sativa</em> L.) often suffers from low temperature during spring rejuvenation. Photosynthetic cold resistance, which is associated with fall dormancy (FD), is crucial for successful rejuvenation. Different dormancy levels of alfalfa (‘Xinmu No. 4’, FD = 3 and ‘Ganong No. 5’, FD = 8) were investigated to study the contribution of photosynthesis to spring rejuvenation of alfalfa, and elucidate the relationship between FD level and cold resistance. The results showed that lower photosynthesis which contributed to the yield and nutritive value of alfalfa, including destroyed chloroplast ultrastructure, limited enzyme activity of Calvin cycle, and the photoinhibition of both PSI and PSII, was showed in alfalfa during the rejuvenation than that in alfalfa aftrer rejuvenation. But cold-induced damage was lighter in low FD alfalfa during rejuvenation. The yield and nutritive value of the first crop were higher in low FD alfalfa. In low FD alfalfa during the rejuvenation, more stable photosynthesis was indicated by the higher stomatal opening, Rubisco activity, Rubisco activating enzyme activity, and net photosynthetic rate, as well as more stable chloroplast ultrastructure. Morover, ETR(I) and ETR (II) were decreased, while the expression of Lhcb2 and D1 proteins were reduced, NPQ and Y(NO) were up-regulated, which indicated electron transfer from PSII to PSI was decreased. Y(ND) and CEF were up-regulated, and the expression of Lhca1 protein was reduced which would reduce the accumulation of excess electrons in PSI. The reduced electron flow from PSII to PSI and increased energy dissipation on the donor side of PSI, as well as a relatively efficient Calvin cycle alleviated PSI photoinhibition. In a word, stable chloroplast ultrastructure, light PSI photoinhibition and relatively efficient Calvin cycle were contributed to cold resistance of photosynthsis in low FD alfalfa during spring rejuvenation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110501"},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokinin, gibberellin and auxin regulate parthenocarpy in cucumber via modulating downstream auxin signaling network","authors":"Li Su,&nbsp;Jiayi Chen,&nbsp;Yu Wu,&nbsp;Songtao Huang,&nbsp;Qingmin Xie,&nbsp;Xuewen Xu,&nbsp;Xuehao Chen,&nbsp;Xiaohua Qi","doi":"10.1016/j.plaphy.2025.110492","DOIUrl":"10.1016/j.plaphy.2025.110492","url":null,"abstract":"<div><div>Parthenocarpy, the development of fruit without pollination and fertilization, provides a solution for ensuring yield stability in adverse environmental conditions. The application of phytohormones in inducing parthenocarpic fruits is an important strategy for horticulture fruit production; however, its inner physiological and molecular mechanisms are less understood. Here, we investigated the hormonal regulation of parthenocarpic fruit formation in cucumber. Auxin, gibberellic acid (GA), and cytokinin treatments successfully induced fruit formation in cucumber. Significant increase in cell size began 1 day after anthesis, indicating that 1 day after anthesis is the critical stage for fruit set. Inner indole-3-acetic acid (IAA) and GA levels were enhanced in parthenocarpic fruit, while no significant changes were observed in cytokinin, abscisic acid, salicylic acid, and jasmonic acid levels. Hormone-induced parthenocarpic fruit showed elevated expression of both auxin signaling and GA biosynthesis genes, and inhibited levels of ABA signaling genes. These findings indicate that auxin and GA play central roles in parthenocarpic fruit formation. These findings also reveal the possibility of improving fruit parthenocarpy through gene editing of auxin signaling and GA biosynthesis genes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110492"},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticles amplify mercury toxicity in rice: Impacts on germination, growth, and cellular integrity","authors":"Huijuan Yu ,&nbsp;Penggang Pei ,&nbsp;Chao Wang ,&nbsp;Tao Sun ,&nbsp;Jianwei Chang ,&nbsp;Xingru Wang ,&nbsp;Shihang Wu ,&nbsp;Zhipeng Hu ,&nbsp;Xiaojia Zhou ,&nbsp;Qingqing Huang ,&nbsp;Yuebing Sun","doi":"10.1016/j.plaphy.2025.110493","DOIUrl":"10.1016/j.plaphy.2025.110493","url":null,"abstract":"<div><div>This study investigates the synergistic co-effects of mercury (Hg) and silver nanoparticles (AgNPs) on rice seed germination and early seedling development. Hg were applied at concentrations of 0, 0.33, 1.65, and 8.25 mg kg⁻¹ while AgNPs were administered at 0, 50, 100, and 500 mg kg⁻¹. Results demonstrate that single low Hg concentrations enhanced germination rate from 79.63 % to 89.51 %, and stimulated α-amylase activity from 0.26 to 0.34 μg g⁻¹ FW min⁻¹. Co-exposure with AgNPs significantly modulated these responses, particularly under 50 and 500 mg kg⁻¹ AgNPs treatments where α-amylase activity reached from 0.26 to 0.49 and 0.25 μg g⁻¹ FW min⁻¹, respectively. The combined treatment with high concentrations of both contaminants further substantially inhibited seedling growth, reducing plant height by 39 % and roots length by 13 %. AgNPs further enhanced Hg accumulation in plant tissues and intensified oxidative stress, as indicated by elevated peroxidase (POD) activity and malondialdehyde (MDA) levels. Ultrastructural and DNA damage analyses confirmed severe cellular disruption and dose-dependent genotoxicity. These findings provide critical insights into the synergistic toxicity mechanisms of Hg and AgNPs co-contamination in rice seedlings, with important implications for agricultural safety under heavy metal and nano-product combined contamination.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110493"},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The plantacyanin gene family in tomato: genome-wide identification, expression analysis, and regulation of salt stress tolerance","authors":"Ruirui Yang ,&nbsp;Zhiyuan Xue ,&nbsp;Jiaxuan Zhu ,&nbsp;Ruili Lv,&nbsp;Yan Li,&nbsp;Xiaoxiao Zhang,&nbsp;Yushi Luan","doi":"10.1016/j.plaphy.2025.110485","DOIUrl":"10.1016/j.plaphy.2025.110485","url":null,"abstract":"<div><div>Phytocyanins (PCs) are a class of plant-specific blue copper proteins that play critical roles in plant development and responses to environmental stresses. Although the <em>PC</em> gene family has been characterized in several plant species, it has not been systematically investigated in tomato, and its role in salt stress tolerance remains largely unknown. In this study, we identified 49 <em>PC</em> genes in tomato. Phylogenetic analysis clustered <em>SlPC</em> gene family into five distinct clades. Collinearity analysis within the <em>SlPC</em> gene family identified five collinear gene pairs. Interspecific collinearity analysis revealed 25 and 53 homologous gene pairs between tomato and <em>Arabidopsis</em>, and tomato and potato, respectively. Intron-exon structure analysis showed that only SlENODL6 is intronless. Promoter analysis indicated the presence of cis-elements associated with growth, development, hormone and stress responses. Expression profiling and qRT-PCR analysis showed that <em>SlSC6</em> expression was induced by salt and abscisic acid treatments. Silencing <em>SlSC6</em> resulted in reduced salt tolerance, evidenced by elevated malondialdehyde and proline content, increased relative electrolyte leakage, and inhibited root growth. Additionally, the transcript levels of <em>SOS1</em>, <em>SOS2</em>, <em>DREB2A</em> and <em>CYP707A2</em> were significantly downregulated, while <em>CYP707A1</em> was significantly upregulated in <em>SlSC6</em>-silenced lines. Silencing also impaired antioxidant enzyme activity and led to increased accumulation of reactive oxygen species (ROS) under salt stress. Overall, these findings suggest that SlSC6 plays a role in conferring salt stress tolerance in tomato. This study lays a foundation for future functional studies of <em>SlPC</em> genes and provides new insights into the molecular mechanisms underlying tomato salt stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110485"},"PeriodicalIF":5.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced glutamine synthetase activity improves grain nitrogen assimilation and low-nitrogen tolerance in sweet corn","authors":"Zexun Yu ,&nbsp;Ang Zhang ,&nbsp;Wei Gao ,&nbsp;Yanfang Sun ,&nbsp;Xiaoyang Yue ,&nbsp;Guangbo Luo ,&nbsp;Da Su ,&nbsp;Liangquan Wu ,&nbsp;Delian Ye","doi":"10.1016/j.plaphy.2025.110499","DOIUrl":"10.1016/j.plaphy.2025.110499","url":null,"abstract":"<div><div>While reducing nitrogen (N) input is essential for sustainable agriculture, maintaining yield under low-N conditions remains a major challenge, particularly for N-sensitive crops such as sweet corn. However, the physiological mechanisms underlying N uptake, allocation, and assimilation in low-N-tolerant sweet corn genotypes remain poorly understood. A two-year field experiment (2022–2023) was conducted using two sweet corn cultivars (MT6855 and TYH6) under four N application rates (0, 90, 180, and 270 kg N ha⁻¹). Low N input significantly reduced the low-N tolerance index, agronomic traits, N accumulation, and N assimilation in both cultivars. Across two years, MT6855 exhibited a higher low-N tolerance index than TYH6 by 164.2 %, 30.8 %, −2.5 %, and 0 % under 0, 90, 180, and 270 kg N ha⁻¹, respectively. MT6855 also showed significantly greater N concentration and accumulation in the ear, together with higher N harvest index and partial factor productivity, despite its comparatively lower plant and ear heights. Notably, MT6855 consistently maintained greater grain free amino acid concentration, as well as higher nitrate reductase (NR) and glutamine synthetase (GS) activities across all N levels and both years. Random forest analysis identified GS (26.6 %) and NR (24.3 %) activities as the primary contributors to low-N tolerance. These findings demonstrate that MT6855 achieves improved low-N tolerance through enhanced N assimilation and preferential allocation to the ear, providing valuable insights for breeding and cultivation strategies to improve low-N tolerance in sweet corn.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110499"},"PeriodicalIF":5.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl jasmonate seed treatment enhances Norway spruce seedling resistance to Botrytis cinerea via a multitude of defense responses","authors":"Ngan Bao Huynh ,&nbsp;Axel Schmidt ,&nbsp;Taina Pennanen ,&nbsp;Jonathan Gershenzon ,&nbsp;Melissa H. Mageroy","doi":"10.1016/j.plaphy.2025.110497","DOIUrl":"10.1016/j.plaphy.2025.110497","url":null,"abstract":"<div><div>Methyl jasmonate (MeJA) is known to effectively protect Norway spruce (<em>Picea abies</em>) against pests and pathogens. However, MeJA application to spruce saplings can significantly reduce growth and is not feasible to use in protecting older trees due to cost. Seed treatment with MeJA or other priming stimulants with fewer negatives could be a practical solution to enhance Norway spruce resistance. Therefore, we assessed the potential of Norway spruce seed treatment with MeJA, pipecolic acid (PipA), lignan (Li), and chitosan (Chi) in enhancing the resistance of the emerged seedlings against <em>Botrytis cinerea</em>. For the first time, MeJA seed treatment was shown to reduce the mortality of the seedlings effectively after <em>B. cinerea</em> infection, with a growth reduction as a side effect. To understand the mechanisms underlying this phenomenon, we quantified phenolics, defense hormones, and differential transcript expressions. MeJA seed treatment increased the concentration of the flavan-3-ols catechin and proanthocyanidin B1. Transcriptomic data suggested an increase in oxidative stress protection, cell wall reinforcement, and pathogenesis-related protein production. Our data also suggested an antagonistic relationship in hormonal signaling between abscisic acid (ABA) and jasmonic acid (JA)/ethylene (ET). Overall, our findings indicated MeJA seed treatment enhanced resistance of young seedlings against <em>B. cinerea</em> via a multitude of defense responses, modulated by complex regulatory systems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110497"},"PeriodicalIF":5.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB gene family in Magnolia biondii: Identification and functional roles in waterlogging stress response","authors":"Yao Chen ,&nbsp;Minglu Yin ,&nbsp;Liyong Sun ,&nbsp;Lina Xu ,&nbsp;Zengfang Yin","doi":"10.1016/j.plaphy.2025.110505","DOIUrl":"10.1016/j.plaphy.2025.110505","url":null,"abstract":"<div><div><em>Magnolia biondii</em>, a deciduous tree of the Magnoliaceae family, is economically important for landscaping and medicine. However, its fleshy roots are highly sensitive to waterlogging, restricting expansion of cultivation area. MYB transcription factors play critical roles in plant abiotic stress response, while their detailed functions in <em>M. biondii</em> have not been systematically investigated. In this study, 185 MbMYBs were identified in <em>M. biondii</em> genome. Transcriptomic data of waterlogged roots revealed that most of <em>MbMYB</em>s were down-regulated and severe waterlogging induced more differentially expressed <em>MbMYB</em>s than moderate waterlogging. Notably, 113 <em>MbMYB</em>s were identified in <em>M. biondii</em> roots as waterlogging-responsive <em>MbMYB</em>s, including 42 <em>Mb1R-MYB</em>s, 68 <em>MbR2R3-MYB</em>s, and 3 <em>Mb3R-MYB</em>s. In particular, five consistently up-regulated <em>MbMYB</em>s were considered as key regulators in response to waterlogging stress. Collinearity analysis revealed that 113 waterlogging-responsive <em>MbMYB</em>s exhibited closer relationship with <em>MYB</em>s in basal angiosperms, and 45 of them were generated by gene duplication events under purifying selection. In addition, multiple stress- and hormone-responsive c<em>is</em>-elements were identified in the promoter regions of 113 waterlogging-responsive <em>MbMYB</em>s, which may confer their potential roles in stress response. Functional validation demonstrated that, under waterlogging stress, transgenic plants overexpressing <em>MbMYB146</em> and <em>MbMYB155</em> exhibited lower levels of H₂O₂ and MDA, along with more stable antioxidant enzyme activities. These findings suggest that <em>MbMYB146</em> and <em>MbMYB155</em> enhance waterlogging tolerance by maintaining ROS homeostasis through enzymatic antioxidant systems, thereby alleviating lipid peroxidation. Overall, our results provide preliminary insights into the role of MbMYBs in waterlogging stress responses and lay a foundation for the molecular breeding and genetic improvement of <em>M. biondii</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110505"},"PeriodicalIF":5.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}