Plant Physiology and Biochemistry最新文献

{"title":"Integrative analysis of the R2R3-MYB gene family revealed that BsMYB36 and BsMYB51 significantly regulate the accumulation of flavonoids in Bletilla striata (Orchidaceae)","authors":"Ruohan Huang ,&nbsp;Yuanqing Chang ,&nbsp;Siting Zheng,&nbsp;Jiaxin Li,&nbsp;Shuai Liu,&nbsp;Junfeng Niu","doi":"10.1016/j.plaphy.2025.109733","DOIUrl":"10.1016/j.plaphy.2025.109733","url":null,"abstract":"<div><div>The R2R3-MYB transcription factors constitute a critical family involved in a variety of biological processes. They have been found to be essential participants in flavonoid biosynthesis in various plants. <em>Bletilla striata</em> (Thunb.) Reichb. f. is an orchid species rich in flavonoid compounds, with anti-inflammatory and antioxidant properties. In this study, we identified 94 R2R3-MYB genes, 89 of them were classified into 22 subgroups, and 92 were mapped to 16 chromosomes. The S5 and S7 subfamilies contained three and four members, respectively which might play roles in the biosynthesis of anthocyanin, proanthocyanidin, and flavonoid. Additionally, BsR2R3-MYBs exhibited tissue-specific expression. There were 36 genes, and 35 genes exhibited high expression in roots and pseudobulbs, respectively. The 25 R2R3-MYB genes from different subfamilies showed varying responses to drought, low temperature, and MeJA treatments. Furthermore, the S5 subfamily member <em>BsMYB51</em> and the S7 subfamily member <em>BsMYB36</em> were heterologous expressed in <em>A.thaliana</em>. Phenotypic observations of <em>A</em>.<em>thaliana</em> showed that <em>BsMYB36</em> and <em>BsMYB51</em> could compensate for the growth differences caused by the <em>atmyb12</em> and <em>atmyb123</em> mutations, respectively. Moreover, the overexpression of <em>BsMYB36</em> increased flavonoid content, while decreasing the accumulation of anthocyanin and proanthocyanidin in <em>A</em>.<em>thaliana</em>. The overexpression of <em>BsMYB51</em> promoted the accumulation of flavonoid, anthocyanin, and proanthocyanidin. Overexpression of <em>BsMYB36</em> and <em>BsMYB51</em> significantly upregulated relative genes in the phenylpropanoid and flavonoid biosynthesis pathways, such as <em>PAL, CHS, F3′H,</em> and <em>DFR</em>. This study provids the foundation for exploring the regulation of flavonoid content by BsMYBs in <em>B.striata</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109733"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOUSLED KINASE INTERACTING PROTEIN 1 (TKI1) interacts with SIN3-LIKES (SNLs) to promote flowering in Arabidopsis","authors":"Chengcheng Zhu ,&nbsp;Chuanyou Chen ,&nbsp;Xia Gong,&nbsp;Haitao Li,&nbsp;Yan Li,&nbsp;Biaoming Zhang,&nbsp;Haitao Zhang,&nbsp;Wenya Yuan","doi":"10.1016/j.plaphy.2025.109761","DOIUrl":"10.1016/j.plaphy.2025.109761","url":null,"abstract":"<div><div>TOUSLED KINASE INTERACTING PROTEIN 1 (TKI1) is a SANT/Myb domain-containing protein, which binds DNA and may function as a transcription factor, and is characterized as an interacting protein with TOUSLED (TSL) in <em>Arabidopsis</em>. However, it remains largely unknown what biological functions of <em>TKI1</em> for few reports about <em>TKI1</em> in the literature. Here we first identified that TKI1 interacts with SIN3-LIKEs (SNLs) and the responsible interaction domains are the C-terminal domain of TKI1 and the PAH (Paired Amphipathic Helix) domains of SNLs respectively in yeast. Then, we further confirmed the interactions between TKI1 and SNLs (SNL1–SNL6) <em>in vitro</em> or <em>in vivo</em> using multiple different protein-protein interaction methods. In addition, <em>TKI1</em> and <em>SNL3</em> are co-expressed in all the examined tissues here, and TKI1 and SNL3 are co-localized in the nucleus, indicating they may function together in plant. Furthermore, Genetic analysis with knockout mutants showed that both <em>TKI1</em> and <em>SNLs</em> promote flowering with an additive effect in long days (LDs), however <em>TKI1</em> induces flowering but <em>SNLs</em> inhibit flowering in short days (SDs). Finally, the flowering repressor <em>FLOWERING LOCUS C</em> (<em>FLC</em>) and its homolog <em>MADS AFFECTING FLOWERING 4</em> (<em>MAF4</em>) were up-regulated, and the flowering activator <em>FLOWERING LOCUS T</em> (<em>FT</em>) and <em>CONSTANS</em> (<em>CO</em>) were down-regulated in <em>tki1</em>, <em>snl1/2/3/4/5</em> and <em>snl1/2/3/4/5 tki1</em> mutants, compared with Col-0. Therefore, our results increase our understanding of the biological functions of <em>TKI1</em>, and reveal that TKI1 physically interacts with SNLs and they both induce flowering in LDs, and indicate that <em>TKI1</em> and <em>SNLs</em> may function together to regulate flowering gene expression to promote flowering in <em>Arabidopsis.</em></div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109761"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies for plant-microbe symbiosis: Mycorrhizal fungi and helper bacteria to improve cold tolerance in rice","authors":"Feng Shi ,&nbsp;Xue He ,&nbsp;Meng Cao ,&nbsp;Ruotong Wu ,&nbsp;Bo Zhang ,&nbsp;Tianle Xu ,&nbsp;Mingguo Jiang ,&nbsp;Fuqiang Song","doi":"10.1016/j.plaphy.2025.109741","DOIUrl":"10.1016/j.plaphy.2025.109741","url":null,"abstract":"<div><div>Cold stress is a limiting factor for rice yield. Empirical evidence has demonstrated that arbuscular mycorrhizal fungi (AMF) can bolster the cold resilience of plants. In barren environments, AMF can promote host plant growth and resistance. However, whether the addition of mycorrhizal helper bacteria (MHB) can further enhance AMF's ability to improve cold tolerance in plants remains unclear. In this study, we set up an uninoculated group, a separately inoculated group, and a compound inoculated group and incubated rice at 25 °C until the three-leaf stage, and then each group was equally divided into four portions for treatment at 25 °C, 12 °C, 8 °C, and 4 °C, respectively. The results showed that: (1) Under cold stress conditions, the biomass of rice plants inoculated with AMF was significantly higher than that of the non-inoculated group; (2) AMF and MHB effectively activated the antioxidant enzyme system in rice plants and improved their osmoregulatory capacity under cold stress; (3) The presence of AMF and MHB stimulated and modulated the upregulation of genes related to photosynthesis and cold tolerance in rice plants, thereby enhancing their resilience against cold stress. Our findings corroborate that MHB can further enhance the cold tolerance of rice by promoting the functions of AMF. This study lays the foundation for expanding rice cultivation areas, and ensuring food production security.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109741"},"PeriodicalIF":6.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the two-component system gene family and the positive role of CsRR5 in cold stress response in tea plants","authors":"Xiaobin Dong ,&nbsp;Changqing Ding ,&nbsp;Xuening Zhang ,&nbsp;Lei Lei ,&nbsp;Yao Chen ,&nbsp;Qianyuan Fu ,&nbsp;Ying Yang ,&nbsp;Yuwan Hao ,&nbsp;Meng Ye ,&nbsp;Jianming Zeng ,&nbsp;Xinchao Wang ,&nbsp;Wenjun Qian ,&nbsp;Jianyan Huang","doi":"10.1016/j.plaphy.2025.109739","DOIUrl":"10.1016/j.plaphy.2025.109739","url":null,"abstract":"<div><div>The two-component system (TCS), a ubiquitous signaling network consisting of histidine kinases (HKs), phosphotransfers (HPs), and response regulator proteins (RRs), participates in various functions, including responses to abiotic stresses. However, a comprehensive identification of <em>TCS</em> genes in tea plants is still lacking. Here, we identified 60 CsTCS members in tea plants, including 23 HKs, 10 HPs, and 27 RRs. The analysis of promoter <em>cis</em>-regulatory elements indicated that <em>CsTCS</em> genes are involved in phytohormone signaling, stress responses, and growth and development. The expression of <em>CsETR1</em>, <em>CsETR2b</em>, <em>CsERS1b</em>, and <em>CsEIN4b</em> from the HK subfamily was down-regulated by ethylene, whereas <em>CsHK2b</em> and <em>CsHK3a</em> were down-regulated by cytokinin. Conversely, <em>CsHK4a</em> and <em>CsHK4b</em> were up-regulated by cytokinin. <em>CsTCS</em> genes were widely expressed in various tissues, with the majority associated with multiple stresses. Furthermore, we demonstrated that suppressing the type-A response regulator <em>CsRR5</em> in tea plants reduced cold tolerance and the expression of <em>CBF</em>-<em>COR</em> pathway genes, indicating that <em>CsRR5</em> positively regulates the cold stress response through the <em>CBF</em> pathway. Therefore, our study establishes a connection between the two-component system and its downstream regulation in tea plant cold stress response.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109739"},"PeriodicalIF":6.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular ATP receptors P2Ks are involved in regulating systemic calcium of Arabidopsis in response to local NaCl stress","authors":"Yuejing Zhang ,&nbsp;Xin Wang ,&nbsp;Mengting Da ,&nbsp;Hetian Sang ,&nbsp;Wenliang Li ,&nbsp;Yao Guo ,&nbsp;Hailong Pang ,&nbsp;Lingyun Jia ,&nbsp;Kun Sun ,&nbsp;Ji Zhang ,&nbsp;Hanqing Feng","doi":"10.1016/j.plaphy.2025.109734","DOIUrl":"10.1016/j.plaphy.2025.109734","url":null,"abstract":"<div><div>Extracellular ATP (eATP) and its receptors play important roles in the regulation of the responses of plants to environmental stimulation. In the present work, application of NaCl to the roots of Arabidopsis (<em>Arabidopsis thaliana</em>) seedlings triggered an increase of Ca²⁺ level not only in the stressed root but also in the systemic organs distant from the root, including the hypocotyl and cotyledon, and the eATP level also was increased in the stressed root and those systemic organs under such local salt stress. Application of exogenous ATP to the root also evoked local and systemic increase of Ca²⁺ level in the seedlings. The lectin receptors kinase I.9 (P2K1) and I.5 (P2K2) are known as the receptors of eATP in Arabidopsis. By comparison of wild-type and P2Ks mutant plants (including <em>p2k1</em> single-mutant, <em>p2k2</em> single-mutant, and <em>p2k1p2k2</em> double-mutant plants), it was found that the local and systemic increases of Ca²⁺ level in the response to local salt stress were weakened by the mutation of P2Ks. In addition, the mutation of P2K1 and P2K2 also attenuates the systemic resistance of cotyledon to salt stress, which was induced by the application of NaCl to the root. These results indicate that the local and systemic Ca²⁺ response to local salt stress is associated with eATP/P2Ks signaling in the plant.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109734"},"PeriodicalIF":6.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic plasticity of Eurohypnum leptothallum in degraded karst ecosystems: Adaptative mechanisms and ecological functions driven by warming temperatures","authors":"Yan Lu ,&nbsp;Dong Chen ,&nbsp;Minghao Deng ,&nbsp;Guanting Guo ,&nbsp;Yifei Wu ,&nbsp;Hengbin Zhang ,&nbsp;Xiaona Li","doi":"10.1016/j.plaphy.2025.109745","DOIUrl":"10.1016/j.plaphy.2025.109745","url":null,"abstract":"<div><div>Phenotypic plasticity is a critical mechanism for plants to adapt to rapid climate change and other global change drivers. <em>Eurohypnum leptothallum</em> is widely distributed in fragile subtropical karst ecosystems, exhibiting strong drought tolerance, water retention, and soil stabilization capabilities, playing a vital ecological role in nutrient cycling and ecological restoration. Our study investigated the specific manifestations of phenotypic plasticity in epilithic <em>E. leptothallum</em> within degraded karst ecosystems. Results showed that <em>E. leptothallum</em> exhibited high phenotypic plasticity in the heterogeneous environments of degraded karst ecosystems. In the temperature range of 21.5 °C–59.5 °C, <em>E. leptothallum</em> developed a set of adaptive mechanisms in response to warming temperatures through the trade-offs and combinations in most morphological traits (increasing in shoot height, stem cortical ratio and leaf middle cell lumen area, decreasing in stem diameter and stem central strand ratio, making leaf shape, cell shape and lumen shape tend to ellipse) and physiological traits (increasing in C, Ca, C:N, C:P, N:P, Fv/Fm and Y(NO), decreasing in qP). Furthermore, these phenotypic variations may confer certain ecological benefits to the degraded karst ecosystems and are expected to contribute to the maintenance and sustainable development of structural stability and species diversity in degraded karst ecosystems and even global ecosystems in the early stages of global warming. The findings provide a new perspective for exploring the response of bryophytes to environmental changes, a theoretical basis for predicting the adaptive strategies of <em>E. leptothallum</em> and its potential ecological functions to degraded karst ecosystems under global warming.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109745"},"PeriodicalIF":6.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the transcriptional regulatory mechanisms of basic helix–loop–helix transcription factors for fine-tuning target genes in rice","authors":"Sunok Moon ,&nbsp;Xu Jiang ,&nbsp;Heebak Choi,&nbsp;Sun-Hwa Ha,&nbsp;Ki-Hong Jung","doi":"10.1016/j.plaphy.2025.109696","DOIUrl":"10.1016/j.plaphy.2025.109696","url":null,"abstract":"<div><h3>Background</h3><div>In the intricate landscape of gene regulation, basic helix-loop-helix (bHLH) transcription factors (TFs) play a pivotal role in controlling gene expression across various biological processes in plants. The bHLH domain, about 60 amino acids long, consists of a DNA-binding basic region and a dimerization helix-loop-helix region. In rice, 188 bHLH proteins are encoded and more than 90 functionally characterized. To finely regulate the expression of various target genes, bHLH TFs engage multiple transcriptional regulatory mechanisms.</div></div><div><h3>Aim of review</h3><div>The aim of this review is to provide a comprehensive understanding of the diverse transcriptional regulatory mechanisms of bHLH TFs in rice.</div></div><div><h3>Key scientific concepts of review</h3><div>bHLH TFs engage the diverse transcriptional regulatory mechanisms, including spatiotemporal expression, the formation of inhibitory complexes, and the integration multiple signaling pathways. Additionally, the ability to switch interaction partners provides flexibility in target site recognition, allowing bHLH proteins regulate a wide range of biological processes, from basic cellular functions to complex developmental pathways. Understanding of multiple transcriptional regulatory mechanisms of bHLH TFs can provide key insights for improving crop traits, such as stress resistance and growth efficiency, which are crucial for enhancing agricultural productivity in the future.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109696"},"PeriodicalIF":6.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The salt and ABA inducible transcription factor gene, SlAITR3, negatively regulates tomato salt tolerance","authors":"Xinsheng Wang ,&nbsp;Zechun Huo ,&nbsp;Li Ma ,&nbsp;Siying Ou ,&nbsp;Meng Guo","doi":"10.1016/j.plaphy.2025.109735","DOIUrl":"10.1016/j.plaphy.2025.109735","url":null,"abstract":"<div><div>It was of great significance for genetic improvement of salt-tolerant crops and increasing the yield of saline-alkali land to excavate salt stress response genes and clarify their molecular mechanism of regulating salt tolerance. Plant-specific transcription factor (TF) ABA-induced transcription repressors (AITRs) played important roles in salt stress. Nevertheless, the underlying mechanisms of how tomato AITRs (SlAITRs) regulate salt stress remain to be elucidated. In this study, we systematically described the characteristics of tomato SlAITR3 and its function in regulating salt tolerance. SlAITR3 was a transcriptional repressor located in nucleus, and <em>SlAITR3</em> gene was induced by salt stress and abscisic acid (ABA). Tomato <em>SlAITR3</em> silencing and knockout improved the salt tolerance. Compared with wide-type (WT) plants, the Na⁺/K⁺ ratio, cell membrane permeability and reactive oxygen species (ROS) were lower in <em>SlAITR3</em> silencing or knockout mutants under salt stress conditions, while the antioxidant enzyme activities were higher. Conversely, the <em>SlAITR3</em>-overexpressing tomato plants showed sensitivity to salt stress. RNA-seq analysis suggested that SlAITR3 might function by regulating stress response genes, especially key genes involved in ion homeostasis and ROS metabolism. In summary, the nuclear localization TF SlAITR3 protein negatively regulated tomato salt tolerance. Our results provided a potential target and a new theoretical reference for the genetic improvement of tomato salt tolerance by biotechnology.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109735"},"PeriodicalIF":6.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of citrus remorin genes identifies an atypical remorin CsREM1.1 responsible for fruit disease resistance","authors":"Jiaming Lei ,&nbsp;Chan Xu ,&nbsp;Rui Li ,&nbsp;Xiaoyan Chen ,&nbsp;Zhengyang Fu ,&nbsp;Juanni Yao ,&nbsp;Zhengguo Li ,&nbsp;Yulin Cheng","doi":"10.1016/j.plaphy.2025.109731","DOIUrl":"10.1016/j.plaphy.2025.109731","url":null,"abstract":"<div><div>Remorins (REMs) are plant-specific proteins associated with plasma membrane (PM) and exhibit diverse biological functions. However, the roles of remorins in fruit crops and fruit disease resistance remain unexplored. Here, we performed a genome-wide characterization of <em>remorin</em> genes from citrus (<em>Citrus sinensis</em>) and identified an atypical remorin CsREM1.1 responsible for fruit resistance to <em>Penicillium digitatum</em> (<em>Pd</em>), a notorious postharvest fungal pathogen of citrus. Ten <em>remorin</em> genes were identified in the <em>C. sinensis</em> genome and they were categorized into five groups. A lot of <em>cis</em>-elements involved in hormone and stress responsiveness were exhibited in the promoter regions of citrus <em>remorin</em> genes. Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the majority of citrus <em>remorin</em> genes, especially <em>CsREM1.1</em>, were significantly induced in citrus fruit upon <em>Pd</em> infection. Unlike typical remorins, CsREM1.1 exhibited nuclear localization in addition to its traditional PM localization. Transient expression of <em>CsREM1.1</em> in the model plant <em>Nicotiana benthamiana</em> suppressed plant cell death triggered by BAX, an important pro-apoptotic factor, and enhanced plant resistance to <em>Botrytis cinerea</em>. Moreover, transient overexpression or silencing of <em>CsREM1.1</em> in citrus fruit indicated the important contribution of CsREM1.1 in fruit resistance to <em>Pd</em>. Our study increases the understanding of plant remorins and provides valuable insights for future research on fruit disease resistance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109731"},"PeriodicalIF":6.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PnOPR6 from Antarctic moss mediates JA-ABA crosstalk and enhances abiotic stress tolerance in Arabidopsis","authors":"Jing Wang ,&nbsp;Fenghua Fan ,&nbsp;Yu Zhao ,&nbsp;Han Li ,&nbsp;Shenghao Liu ,&nbsp;Guangyao Li ,&nbsp;Pengying Zhang","doi":"10.1016/j.plaphy.2025.109730","DOIUrl":"10.1016/j.plaphy.2025.109730","url":null,"abstract":"<div><div>Jasmonates (JAs) and abscisic acid (ABA) are vital plant hormones that are integral to the plant's response mechanisms against various abiotic stresses. These hormones also function in an antagonistic manner to regulate seed germination and dormancy. However, little is known about the molecular mechanism underlying the interaction between ABA and JA signaling. Here, seven 12-oxo-phytodienoic acid reductase genes (<em>PnOPR1-7</em>), a key enzyme in the JA biosynthesis pathway, were identified in the Antarctic moss <em>Pohlia nutans</em> transcriptome, and their expressions in response to abiotic stress were examined. Among these, <em>PnOPR6</em> expression levels rose most under cold and UV-B stresses. Transgenic <em>Arabidopsis</em> overexpressing <em>PnOPR6</em> demonstrated increased tolerance to salt, cold, dehydration, glucose, and ABA, but also greater sensitivity to methyl jasmonate (MeJA) during seed germination or early root growth. Furthermore, in the transgenic <em>Arabidopsis</em>, <em>PnOPR6</em> suppressed the expression of genes involved in the ABA pathway and ABI3/5-responsive JA receptor <em>COI1</em>. Additionally, phytohormone metabolomics investigations revealed a significant rise in JA precursor (OPDA, OPC-6, and OPC-4), JA, and its derivative 12-OH-JA in <em>PnOPR6</em>-overexpressing line. Moreover, the accumulation of flavonoid in <em>Arabidopsis</em> was enhanced by heterologous expression of <em>PnOPR6.</em> These findings imply that <em>PnOPR6</em> functions as a signaling regulator, improving plant resistance to abiotic stress through flavonoid accumulation and JA-ABA antagonistic crosstalk, therefore aiding <em>P. nutans</em> in adjusting to polar climates.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109730"},"PeriodicalIF":6.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}