Plant Physiology and Biochemistry最新文献

{"title":"Exogenous application of wood vinegar improves rice yield and quality by elevating photosynthetic efficiency and enhancing the accumulation of total soluble sugars","authors":"Ghulam Hur ,&nbsp;Maleeha Ashraf ,&nbsp;Muhammad Yousaf Nadeem ,&nbsp;Rao Saad Rehman ,&nbsp;Hla Myo Thwin ,&nbsp;Khubaib Shakoor ,&nbsp;Mahmoud F. Seleiman ,&nbsp;Majed Alotaibi ,&nbsp;Bao-Zhong Yuan","doi":"10.1016/j.plaphy.2024.109306","DOIUrl":"10.1016/j.plaphy.2024.109306","url":null,"abstract":"<div><div>Rice is an important cereal crop for over half of the world population and essential for food security, especially in developing countries where it constitutes a substantial part of daily caloric intake. Wood vinegar, obtained from biomass pyrolysis and rich in organic acids, phenols, esters, sugars, and alcohols, promotes crop growth and productivity. Its diverse composition offers an eco-friendly and cost-effective growth promoter and improves stress tolerance in different crops; however, its optimal application timings in rice cultivation remain underexplored. Our study investigated the effects of wood vinegar on rice growth, photosynthetic efficiency, chlorophyll contents, soluble sugars, phenolics, grain chalkiness, and yield at different stages and combinations such as tillering (T), jointing (J), flowering (F), grain filling (G), T + J, T + F, T + G, J + F, J + G, F + G, T + J + F, T + J + G, T + F + G, and J + F + G. Our results indicate that wood vinegar application at T + J + F stages significantly enhanced the photosynthetic rate by promoting gaseous exchange, chlorophyll contents, and SPAD index when compared with control. Additionally, total soluble sugars (9.71 mg g⁻¹) and phenolics (2.4 mg g⁻¹) levels were noticeably induced during wood vinegar application as compared with corresponding control. Besides, this treatment also elevated the yield by up to 32.4%, reduced grain chalkiness, and enhanced head rice percentage. Taken together, these findings suggest that wood vinegar supports sustainable agriculture by improving crop yield and quality, offering considerable benefits to farmers.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"218 ","pages":"Article 109306"},"PeriodicalIF":6.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic efficiency of three C4 species is maintained under low light and high vapour pressure deficit","authors":"Ashraf Muhammad Arslan ,&nbsp;Yi Ning Xu ,&nbsp;Xuming Wang ,&nbsp;Bo Ya Liu ,&nbsp;Lei Li ,&nbsp;Huixing Kang ,&nbsp;Xiao Ying Gong","doi":"10.1016/j.plaphy.2024.109304","DOIUrl":"10.1016/j.plaphy.2024.109304","url":null,"abstract":"<div><div>The photosynthetic efficiency of C₄ plants could be impaired in environments with low light and high vapour pressure deficit (VPD). However, the interactive effect of low light and high VPD on C₄ photosynthetic efficiency remains unexplored. We grew three C₄ species, <em>Setaria viridis</em> L., <em>Sorghum sudanense</em> (Piper) Stapf., and <em>Zea mays</em> L., under controlled high and low irradiance and VPD conditions in growth chambers. Gas exchange and chlorophyll fluorescence parameters were measured to estimate apparent maximum quantum yield (QY_max), and ¹³C discrimination of leaf dry mass (Δ_DM) was measured to estimate bundle-sheath leakiness (BS-leakiness). Averaged over VPD levels, net CO₂ assimilation rate at saturating light (<em>A</em>_sat) decreased in <em>S</em>. <em>viridis</em> and <em>S</em>. <em>sudanense</em> but not <em>Z</em>. <em>mays</em> under low irradiance. High VPD decreased <em>A</em>_sat of <em>S</em>. <em>viridis</em> but increased <em>A</em>_sat of <em>Z</em>. <em>mays</em>. QY_max was not significantly affected by low light, high VPD or their interaction, which was associated with the unchanged photochemical efficiency of photosystem II. Low light did not significantly influence BS-leakiness, while high VPD only increased BS-leakiness of <em>Z</em>. <em>mays</em> by 0.08 compared with low VPD at low irradiance. The insignificant interaction of low light and high VPD on QY_max and BS-leakiness indicates that these C₄ species could maintain photosynthetic efficiency under these unfavourable conditions.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109304"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of SWEET gene family and the sugar transport function of three candidate genes during female flower bud induction stage of Juglans sigillata Dode","authors":"Wenwen Li ,&nbsp;Jinyan Chen ,&nbsp;Chunxiang Li ,&nbsp;Dong Huang ,&nbsp;Yuanqi Huang ,&nbsp;Wen'e Zhang ,&nbsp;Xuejun Pan","doi":"10.1016/j.plaphy.2024.109288","DOIUrl":"10.1016/j.plaphy.2024.109288","url":null,"abstract":"<div><div>Sugar Will Eventually be Exported Transporter (SWEET) transports sugar to sink organs and regulates intercellular sugar transport to provide energy for plant growth and development. In this study, twenty-two <em>SWEET</em> genes were identified and distributed on eleven chromosomes. Phylogenetic tree analysis showed that these genes could be divided into four subfamilies. Metabolism and transcriptome analysis showed that sucrose and fructose were accumulated in female flower buds at physiological differentiation stage (PDS). The third branch of <em>JsSWEET1</em> and the fourth branch of <em>JsSWEET9</em> and <em>JsSWEET17</em> were highly expressed in female flower buds at undifferentiated stage (UDS) and PDS, which promoted sugar accumulation in female flower bud differentiation, so these three candidate <em>SWEET</em> genes were considered to be involved in the accumulation of sugar in the flower bud differentiation of <em>Juglans sigillata</em>. The subcellular localization showed that all three candidate genes were located on the cell membrane, and <em>JsSWEET17</em> was also expressed and functioned in the vacuolar membrane. Through overexpression in callus and silencing in female flower buds at UDS and PDS, it was found that <em>JsSWEET1</em> positively regulated the accumulation of sucrose in female flower buds, and <em>JsSWEET9</em> and <em>JsSWEET17</em> are involved in the transport and accumulation of fructose during flower bud differentiation. These results could provide a comprehensive understanding of the <em>JsSWEETs</em> gene family and provide theoretical guidance for further study of the function of <em>SWEET</em>-induced sugar accumulation in plant flower development.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109288"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZnO nanoparticles enhances cadmium tolerance by modulating N6-methyladenosine (m6A) level of stress-responsive genes NRT1 and GM35E in vegetable soybean","authors":"Bin Wang ,&nbsp;Ruiqiu Fang","doi":"10.1016/j.plaphy.2024.109303","DOIUrl":"10.1016/j.plaphy.2024.109303","url":null,"abstract":"<div><div>Cadmium (Cd) is a hazardous heavy metal pollutant that poses significant risks to agricultural production and human health. Nanoparticles (NPs) can alleviate the effects of cadmium on crops by regulating the expression of stress-responsive genes, however, the mechanism of regulation is unknown. N6-methyladenosine (m6A) is a prevalent RNA modification, which determines the expression level of RNA. In this study, we performed m6A methylome analysis and gene editing to investigate the regulation of stress-responsive genes by m6A under Cd stress with ZnO nanoparticles (ZnO NPs). Firstly, we identified 16 differentially expressed genes (DEGs) with differential m6A-modification by m6A methylome seq, which included 6 stress-responsive genes. ZnO NPs treatment reduced the m6A level and stabilized the mRNAs of these stress-responsive genes. Then, we utilized the <em>in vivo</em> m6A modification tool, Plant m6A Editors (PMEs), specifically reduced the m6A level of <em>NRT1</em> and <em>GM35E</em> in transgenic plants. The expression of <em>NRT1</em> and <em>GM35E</em> was increased, and plants carrying PMEs-NRT1 and PMEs-GM35E showed stronger Cd tolerance than control. Therefore, we can conclude that NPs enhance Cd tolerance in plants by regulating the m6A methylation level of stress-responsive genes such as <em>NRT1</em> and <em>GM35E</em>, which ultimately affects the expression of these genes. This study proposed a post-transcriptional regulatory network in vegetable soybean roots responding to Cd with ZnO NPs, potentially offering new insights into gene manipulation for controlling low Cd accumulation in crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109303"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Transcriptome and Mass Spectrometry Analysis Reveals Novel Cyclotides with Antimicrobial and Cytotoxic Activities from Viola arcuata","authors":"Xue Tang ,&nbsp;Qiongyan Zou ,&nbsp;Yujiao Yan ,&nbsp;Fawei He ,&nbsp;Yunfei Cui ,&nbsp;Yuanyuan Lian ,&nbsp;Dongting Zhangsun ,&nbsp;Yong Wu ,&nbsp;Sulan Luo","doi":"10.1016/j.plaphy.2024.109300","DOIUrl":"10.1016/j.plaphy.2024.109300","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109300"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction effect of water deficit stress and nanosilicon on phytochemical and physiological characteristics of hemp (Cannabis sativa L.)","authors":"Ayyub Rezghiyan ,&nbsp;Hassan Esmaeili ,&nbsp;Mohsen Farzaneh ,&nbsp;Hassan Rezadoost","doi":"10.1016/j.plaphy.2024.109298","DOIUrl":"10.1016/j.plaphy.2024.109298","url":null,"abstract":"<div><div>Different practical approaches have been employed to attenuate the destructive impacts of water deficit stress on plants, such as utilization of humic acid, salicylic acid, algae extract, mulching, and microorganisms, as well as silicon application. Nanosilicon significantly moderates the ruinous effects of abiotic and biotic stress in plants through some physiological processes. In this study, the interaction effect of drought stress and nanosilicon on phytochemical and physiological characteristics of hemp (<em>Cannabis sativa</em> L.) was investigated, wherein the four-week-old seedlings were subjected to irrigation treatments at four levels, including 100% (control), 80% (mild stress), 60% (moderate stress), and 40% (severe stress) of field capacity and nanosilicon at three concentrations (0, 0.5, and 1.5 mM) was foliar applied every 10 days in a factorial completely randomized design experiment with three replications for 30 days. Phytochemical and physiological analyses such as photosynthetic pigments, total phenolic and flavonoid content, and antioxidant enzyme activities were conducted. The results indicated that the highest content of Cannabidiol and Tetrahydrocannabinol was achieved using 1.5 mM (1.89%) and 0.5 mM (0.63%) nanosilicon treatments, respectively, under moderate stress. The plants subjected to severe drought stress without nanosilicon application displayed the lowest values of chlorophyll <em>a</em> (0.50 mg/g FW) and <em>b</em> (0.20 mg/g FW). The use of nanosilicon excited the activation of antioxidant enzymes, wherein the plants treated with nanosilicon and drought stress exhibited significantly higher SOD, POD, and APX activities compared to the control. Under all drought stress levels, foliar application of nanosilicon at the highest concentration decreased proline content. The results proposed that the application of 1.5 mM nanosilicon, as a more efficient concentration, improved drought tolerance in hemp plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109298"},"PeriodicalIF":6.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize yield is associated with abscisic acid and water potential under reduced soil water supply but with indoleacetic acid in genotypic renewal","authors":"Qi Liao ,&nbsp;Xukai Liang ,&nbsp;Ruopu Wang ,&nbsp;Taisheng Du ,&nbsp;Xiao Zhao ,&nbsp;Shaozhong Kang ,&nbsp;Ling Tong ,&nbsp;Risheng Ding","doi":"10.1016/j.plaphy.2024.109299","DOIUrl":"10.1016/j.plaphy.2024.109299","url":null,"abstract":"<div><div>Irrigation and breeding are important practices for improving yield and water use efficiency of maize (<em>Zea mays</em> L.) in arid regions. However, the physiological mechanisms of yield under varying water supplies and genotypes remain unclear. Here, we examine the different physiological mechanisms underlying maize yield responses to varying soil water supplies and three genotypes (MC670, ZD958, and ZD2#) cultivated in northwestern China over the past five decades. The declining water supply significantly reduced maize leaf hydraulic transport, stomatal conductance (<em>g</em>_s), net photosynthetic rate (<em>A</em>), yield, kernel number, biomass, and evapotranspiration (<em>ET</em>). Conversely, it led to an increase in abscisic acid (ABA), hydrogen peroxide, intrinsic water use efficiency, and water productivity. Interestingly, there was no significant impact on indoleacetic acid (IAA), thousand kernel weight, or harvest index (<em>HI</em>). Breeding efforts increased leaf IAA levels, biomass, thousand kernel weight, yield, <em>HI</em>, and water productivity without altering physiological traits or <em>ET</em>. The superior yield of MC670 could be attributed to a simultaneous enhancement in both kernel number and thousand kernel weight, while ZD958 exhibited greater yield stability. ABA and hydraulic traits (predawn leaf water potential, leaf water potential, and whole-plant hydraulic conductance) coordinated <em>g</em>_s under reduced soil water supply, while ABA and predawn leaf water potential regulated yield by modulating <em>g</em>_s to affect both <em>A</em> and <em>ET</em>. Breeding for yield gains was associated with IAA-induced enhancements in biomass and <em>HI</em>, independent of key physiological traits (e.g., <em>g</em>_s and <em>A</em>) and <em>ET</em>. The observed increase in water productivity primarily stemmed from notable yield improvements rather than alterations in <em>ET</em>. Hence, the selection of high-yielding genotypes under water-limited and well-watered conditions requires consideration of water-related physiological traits and IAA levels, respectively.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109299"},"PeriodicalIF":6.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating physiological and transcriptomic analyses explored the regulatory mechanism of cold tolerance at seedling emergence stage in upland cotton (Gossypium hirsutum L.)","authors":"Jingyu Zhang ,&nbsp;Ruihua Liu ,&nbsp;Siping Zhang ,&nbsp;Changwei Ge ,&nbsp;Shaodong Liu ,&nbsp;Huijuan Ma ,&nbsp;Chaoyou Pang ,&nbsp;Qian Shen","doi":"10.1016/j.plaphy.2024.109297","DOIUrl":"10.1016/j.plaphy.2024.109297","url":null,"abstract":"<div><div>Cold stress is one of the major abiotic stressor that profoundly impacts plant growth. Cotton, a widely cultivated variety, is particularly susceptible to cold stress. Unraveling the responses to cold stress is critical for cotton demand. In this investigation, we conducted comparative physiological and transcriptomic analyses of the cold-tolerant variety XLZ16 and cold-sensitive variety XLZ84 at seedling emergence stage under cold stress. Following exposure to cold stress, XLZ16 exhibited a markedly higher growth phenotype and increased activity of antioxidant enzymes, while simultaneously showing reduced cellular oxidative damage and apoptosis. Furthermore, the levels of auxin (IAA), cytokinin (CTK), and salicylic acid (SA) significantly increased during cold stress, whereas the contents of catendorsterol (TY), brassinosterone (CS), and jasmonic acid (JA) significantly decreased. Integrated with stoichiometric analysis, these findings definitively demonstrated significant differences in antioxidant capacity and hormone content between the two varieties during their response to cold stress. A total of 6207 potential cold-responsive differentially expressed genes (DEGs) were identified through transcriptome sequencing analysis. Enrichment analyses of these DEGs revealed that pathways related to “hormones biosynthesis and signaling” as well as “circadian rhythm” were associated with cold response. Notably, the hub gene <em>Gh_D12G2567</em> (<em>GhJAZ3</em>), encoding jasmonate ZIM-domain (JAZ) proteins, was found to influence the JA signal transduction pathway and regulate cotton growth under cold stress within the MEred module network. Furthermore, suppressing the expression level of <em>GhJAZ3</em> by virus-induced gene silencing led to the reduction of cold resistance, implying <em>GhJAZ3</em> as a positive regulator of cold tolerance. This study provides valuable insights into the response mechanisms of cotton under cold stress. It also serves as a reference and foundation for further enhancing cold tolerance of new cotton varieties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109297"},"PeriodicalIF":6.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl acetate extract of Artemisia argyi improves the resistance of cotton to Verticillium dahliae by activating the immune response","authors":"Jia Zhou ,&nbsp;Yunhan Wang ,&nbsp;Qiaohuan Chen ,&nbsp;Rong Xu ,&nbsp;Bisheng Huang ,&nbsp;Dahui Liu ,&nbsp;Yuhuan Miao","doi":"10.1016/j.plaphy.2024.109296","DOIUrl":"10.1016/j.plaphy.2024.109296","url":null,"abstract":"<div><div>Verticillium wilt, a significant pathogen affecting cotton, has historically been challenging to control, posing a substantial threat to the sustainable development of the cotton industry. This study demonstrates that resistance to <em>Verticillium dahliae</em> in cotton can be enhanced by treating the roots with an ethyl acetate extract (EAAA) extracted from <em>Artemisia argyi</em>. The mechanisms by which EAAA activates immunity in cotton were elucidated by examining the expression levels of resistance genes post-treatment, evaluating salicylic acid (SA) and jasmonic acid (JA) levels, analyzing transcriptome data, and employing virus-induced gene silencing (VIGS) technology. Additionally, pot experiments were conducted to validate the efficacy of EAAA in controlling Verticillium wilt. The flavonoid content in EAAA was qualitatively analyzed using Ultra-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (UPLC-MS/MS), identifying three specific flavonoids that were further screened to verify their roles in activating cotton immunity. Cotton plants treated with EAAA exhibited reduced leaf chlorosis and browning in the vascular bundles. Genes involved in SA and JA synthesis and signaling in the root system were highly expressed, resulting in increased levels of SA and JA. Transcriptome analysis revealed that most upregulated differentially expressed genes were primarily enriched in the Mitogen-Activated Protein Kinase (MAPK) signaling pathway. Two specific genes, <em>RLK</em> and <em>MAPKKK18</em>, were identified through VIGS technology as key regulators of the immune pathway in cotton. The flavonoid monomer activation experiment demonstrated that eupatilin, hispidulin, jaceosidin, and a mixture of these three could induce the expression of cotton-related resistance genes. Collectively, these findings provide a research basis for the development of EAAA as a natural plant immune-inducing agent against cotton Verticillium wilt.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109296"},"PeriodicalIF":6.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Euonymus maximowiczianus aril-derived long-term suspension-cultured cells: Light and methyl jasmonate impact in the anthocyanin and VLCFA accumulation","authors":"Alexander V. Nosov ,&nbsp;Artem A. Fomenkov ,&nbsp;Roman A. Sidorov ,&nbsp;Sergei V. Goriainov","doi":"10.1016/j.plaphy.2024.109293","DOIUrl":"10.1016/j.plaphy.2024.109293","url":null,"abstract":"<div><div>The genus <em>Euonymus</em> (L.) consists of shrubs and woody plants, distributed mainly in the Northern Hemisphere. Several hundred of secondary metabolites have been isolated from <em>Euonymus</em> spp. In addition, fatty oil was found in the fruits of some <em>Euonymus</em> spp., which accumulates not only in the seeds but also in the arils. This study presents the research of unique over ten-year-old suspension cell cultures of the endemic plant <em>Euonymus maximoviczianus</em> Prokh., obtained from the aril tissue of unripe capsules. The suspension cells retain the ability to form oil droplets containing neutral lipids. Both cells growing in the dark (Em-D culture) and cells growing in the light (Em-L culture) can synthesize very-long-chain fatty acids (VLCFAs) as well as cyanidin-3-<em>O</em>-hexoside, delphinidin-3-<em>O</em>-hexoside, and peonidin-3-<em>O</em>-hexoside. Here, we researched the VLCFA and anthocyanin accumulation dynamics during subcultivation, as well as the influence of methyl jasmonate (MeJA) and light on these processes. In the darkness, the formation of VLCFAs was more intense, while the biosynthesis of anthocyanins was significantly activated in the light. In Em-L cells, more than 76% of anthocyanins were represented by cyanidin-3-<em>O</em>-hexoside, and in Em-D cells delphinidin-3-<em>O</em>-hexoside was more actively synthesized (45%). MeJA substantially enhanced the accumulation of anthocyanins (especially in the light) and, surprisingly, the formation of VLCFAs in both Em-L and Em-D cell cultures. The possible competition between the biosynthetic pathways of VLCFAs and anthocyanins is discussed in connection with the commonality of the cytosolic pool of their precursor, malonyl-CoA.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109293"},"PeriodicalIF":6.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}