Plant Physiology and Biochemistry最新文献_第9页

Understanding salinity tolerance mechanisms in finger millet through metabolomics

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-07 DOI: 10.1016/j.plaphy.2025.109742

Saptarshi Mondal , David Jespersen

{"title":"Understanding salinity tolerance mechanisms in finger millet through metabolomics","authors":"Saptarshi Mondal , David Jespersen","doi":"10.1016/j.plaphy.2025.109742","DOIUrl":"10.1016/j.plaphy.2025.109742","url":null,"abstract":"<div><div>Finger millet (<em>Eleusine coracana</em> Gaertn L.) is an underutilized but nutritionally rich climate resilient food crop that is generally cultivated on marginal lands. Soil salinization is a major abiotic stress that leads to a reduction in growth and yield by affecting various physiological and metabolic processes in plants. The existence of genotypic variation for salt tolerance in finger millet indicates the possibility of crop improvement via plant breeding. The overall objective of the study was to identify metabolic changes associated with improved salt tolerance in finger millet. Understanding tolerance mechanisms plays a pivotal role in the development of elite cultivars. Based on the consensus of several phenotypic data at the germination and seedling stages, we further evaluated two accessions (IE 518 and IE 405) with morphophysiological parameters and metabolomics to dissect the salinity tolerance mechanisms in finger millet. Significant phenotypic separation of IE 518 and IE 405 for salt tolerance was reflected through differences in several physiological processes such as maximum quantum yield of photosystem II (F<sub>V</sub>/F<sub>M</sub>), net photosynthesis rate (P<sub>n</sub>), shoot Na<sup>+</sup> ion accumulation, and oxidative stresses (electrolyte leakage and malondialdehyde content). However, both accessions showed retention of K<sup>+</sup> ions, which underscores the role of ion homeostasis in finger millet. Pathway enrichment analysis with the uniquely salt regulated metabolites identified key metabolic pathways such as stress signaling, biotin metabolism, energy metabolism, amino acid biosynthesis, and sugar metabolism in IE 518. An enhanced accumulation of reducing sugars (mannose and melibiose) and amino acids (L-Proline and GABA) in IE 518 under salinity suggests maintaining osmotic balance as a key tolerance mechanism in finger millet.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109742"},"PeriodicalIF":6.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GmMYB1-GmbHLHA-GmCPC-like module regulates light-induced anthocyanin production in soybean sprouts

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-06 DOI: 10.1016/j.plaphy.2025.109738

Mengyang Niu , Youyou Guo , Gang Hu , Liuyi Li , Yaping Lu , Yanwu Lu , Xingxing Yuan , Zhenguo Shen , Nana Su

{"title":"The GmMYB1-GmbHLHA-GmCPC-like module regulates light-induced anthocyanin production in soybean sprouts","authors":"Mengyang Niu , Youyou Guo , Gang Hu , Liuyi Li , Yaping Lu , Yanwu Lu , Xingxing Yuan , Zhenguo Shen , Nana Su","doi":"10.1016/j.plaphy.2025.109738","DOIUrl":"10.1016/j.plaphy.2025.109738","url":null,"abstract":"<div><div>Soybean (<em>Glycine</em> max L.) is an important economic crop, flavonoids (such as anthocyanins) and some other nutrients of which were significantly promoted after germination. The accumulation of anthocyanin is influenced by many kinds of factors in plants, the regulatory mechanism of which is relatively complex. Here, soybean double mutant <em>stf1/2</em> was utilized and found that GmSTF1/2 participated in light-mediated anthocyanin production in soybean. GmMYB1 was considered as a direct target of GmSTF1/2. Expressing <em>GmMYB1</em> in soybean hair roots and tobacco significantly promoted anthocyanin content. GmMYB1 could directly bind to the promoters of <em>GmDFR</em>, <em>GmANS</em>, and <em>GmUFGT</em>, thereby promoting their transcriptions. In addition, GmMYB1 interacted with GmbHLHA, and their interaction could enhance the functions of GmMYB1 in positively regulating anthocyanin accumulation. R3-MYB <em>GmCPC-like</em> was activated by GmMYB1 when anthocyanin was abundant. Expressing <em>GmCPC-like</em> significantly inhibited anthocyanin contents in soybean hair roots and tobacco. GmCPC-like inhibited anthocyanin accumulation mainly through interacting with GmMYB1 and GmbHLHA, and then decreased their positive roles in anthocyanin production. Taken together, the GmMYB1-GmbHLHA-GmCPC-like module finely regulates anthocyanin production in soybean sprouts.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109738"},"PeriodicalIF":6.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epiphytic bacterial consortia drive growth regulation in potato under methyl jasmonate elicitation: A leaf surface multi-omics perspective

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-06 DOI: 10.1016/j.plaphy.2025.109737

Xiaoting Fang , Peihua Li , Chao Luo , Chao Wu , Xinxin Shi , Jianbin Lan , Fei Zhao , Fang Wang , Kaiqin Zhang , Cuiqin Yang , Zhitong Ren , Shunlin Zheng

{"title":"Epiphytic bacterial consortia drive growth regulation in potato under methyl jasmonate elicitation: A leaf surface multi-omics perspective","authors":"Xiaoting Fang , Peihua Li , Chao Luo , Chao Wu , Xinxin Shi , Jianbin Lan , Fei Zhao , Fang Wang , Kaiqin Zhang , Cuiqin Yang , Zhitong Ren , Shunlin Zheng","doi":"10.1016/j.plaphy.2025.109737","DOIUrl":"10.1016/j.plaphy.2025.109737","url":null,"abstract":"<div><div>Methyl jasmonate (MeJA), a lipid-derived signaling molecule widely reported as a plant growth regulator, was revealed in this study to coordinate oxidative stress adaptation and delay senescence in potato through metabolite-microbe interactions, ultimately improving yield. MeJA triggered leaf oxidative stress while integrating rapid enzymatic scavenging, sustained osmoprotectant accumulation, and membrane stabilization, effectively delaying senescence initiation. Metabolic reprogramming under MeJA suppressed endogenous jasmonic acid synthesis while promoting saturated fatty acid biosynthesis, altering leaf surface lipid composition. These lipid changes, combined with MeJA-induced alkaloids, drove functional restructuring of phyllosphere epiphytic bacteria through fatty acid-mediated niche specialization, enhancing bacterial metabolism and enriching stress-resistant Proteobacteria. Notably, the enrichment of saturated fatty acids correlated with microbial taxa exhibiting specialized lipid metabolism. Field trials demonstrated that 200 μmol/L MeJA optimized redox homeostasis and photosynthetic longevity in early-maturing cultivar 'Favorita', translating delayed senescence into significant yield increases. This study proposes a \"metabolite-guided microbial niche construction\" model, where host lipid metabolism and secondary metabolites jointly shape stress-adapted microbial communities, providing new strategies for precision agrochemical design targeting phyllosphere microbiome engineering.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109737"},"PeriodicalIF":6.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated metabolomic and transcriptomic analyses revealed the overlapping response mechanisms of banana to cold and drought stress

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-06 DOI: 10.1016/j.plaphy.2025.109766

Junchao Xing , Xiaoxue Ye , Kaisen Huo , Zehong Ding , Weiwei Tie , Zhengnan Xie , Chaochao Li , Fanjuan Meng , Wei Hu

{"title":"Integrated metabolomic and transcriptomic analyses revealed the overlapping response mechanisms of banana to cold and drought stress","authors":"Junchao Xing , Xiaoxue Ye , Kaisen Huo , Zehong Ding , Weiwei Tie , Zhengnan Xie , Chaochao Li , Fanjuan Meng , Wei Hu","doi":"10.1016/j.plaphy.2025.109766","DOIUrl":"10.1016/j.plaphy.2025.109766","url":null,"abstract":"<div><div>Banana (<em>Musa</em> spp.), a vital tropical fruit and food crop, faces significant challenges from cold and drought stress, which threaten its productivity. Uncovering the overlapping mechanisms of crop responses to abiotic stresses is essential for the development of multi-resistant crop varieties. This study investigates the overlapping response mechanisms of banana to cold and drought stress through integrated metabolomic and transcriptomic analyses. We conducted physiological assessments alongside these analyses to elucidate shared mechanisms. Our results showed that both cold and drought stress disrupted cell membrane stability and reduced relative water content and chlorophyll content in banana leaves. Metabolomic analysis identified 1800 annotated metabolites, with 636 and 405 differentially accumulated metabolites (DAMs) under cold and drought stress, respectively, and flavonoids represented the most abundant metabolite class. Transcriptomic analysis revealed that 5687 differentially expressed genes (DEGs) were induced under both stress conditions, with significant enrichment in pathways related to ascorbic acid, arginine, and proline metabolism. Integrating metabolomic and transcriptomic data highlighted carbohydrate, amino acid, and flavonoid metabolism as the central pathways shared in response to cold and drought stresses. Notably, while these pathways were common, specific structural genes and accumulated metabolites varied between stress types. Additionally, our results suggest that GDP-mannose is the primary ascorbate synthesis route under cold stress, whereas myo-inositol and galacturonic acid pathways dominate under drought stress. These findings enhance our understanding of banana's adaptive responses and provide a foundation for breeding multi-stress-resistant crop varieties in an era of climate change.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109766"},"PeriodicalIF":6.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural basis for the dual roles of DPW in lipid and UDP-sugar metabolism during rice anther development

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109762

Shanshan Qu , Jin Wang , Gang Li , Chen Miao , Liming Yan , Wei Wang

{"title":"Structural basis for the dual roles of DPW in lipid and UDP-sugar metabolism during rice anther development","authors":"Shanshan Qu , Jin Wang , Gang Li , Chen Miao , Liming Yan , Wei Wang","doi":"10.1016/j.plaphy.2025.109762","DOIUrl":"10.1016/j.plaphy.2025.109762","url":null,"abstract":"<div><div>Fatty acids and uridine diphosphate (UDP)-sugars are essential metabolites involved in the biosynthesis of polysaccharides and lipids, both of which are critical for anther development in plants. Our previous study identified Defective Pollen Wall (DPW), a rice fatty acyl carrier protein reductase (FAR), as a key factor in pollen wall formation. In this study, we demonstrate that the structure of DPW in complex with its cofactor NADP<sup>+</sup> exhibits structural similarities to that of UDP-glucose epimerase (UGE). In <em>vitro</em> enzymatic assays utilizing recombinant DPW confirmed its ability to interconvert UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal) in an NADP(H)-dependent manner. Mutations in conserved NADP(H)-binding residues abolished both DPW's FAR and UGE activities. <em>In vivo</em> assays showed that the <em>dpw</em> mutation causes UDP-Glc accumulation, disrupting the balance between UDP-Glc and UDP-Gal in rice anthers. Taken together, our findings provide insights into the dual roles of DPW in lipid and UDP-sugar metabolism during rice anther development, shedding light on how plants integrate metabolic pathways through multifunctional enzymes to regulate male reproductive development.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109762"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developed BL-EF to acquire plant growth-promoting functions under salt stress by introducing the ACC deaminase gene

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109764

Xinyu Jia , Linlin Sun , Junsong Yue , Xiaohu Zhou , Zihe Deng , Xiaochen Liu , Zhansheng Wu

{"title":"Developed BL-EF to acquire plant growth-promoting functions under salt stress by introducing the ACC deaminase gene","authors":"Xinyu Jia , Linlin Sun , Junsong Yue , Xiaohu Zhou , Zihe Deng , Xiaochen Liu , Zhansheng Wu","doi":"10.1016/j.plaphy.2025.109764","DOIUrl":"10.1016/j.plaphy.2025.109764","url":null,"abstract":"<div><div>The application of plant growth-promoting rhizobacteria (PGPR) is a novel and effective strategy to ameliorate soil salinity and increase agricultural productivity. ACC deaminase (ACCD) in PGPR plays a key role in alleviating salt stress and promoting plant growth. This study aimed to investigate the potential of ACCD-producing strain BL-EF to mitigate salt stress in tomato plants. The ACCD gene was introduced into the non-PGPR <em>Escherichia coli</em> to successfully construct to construct BL-EF and produce catalytically active ACCD. The results showed that strain BL-EF significantly increased the height of tomato plants by 30.94% and 44.63%, under both normal and salt stress conditions, respectively. Strain BL-EF also modulated the photosynthetic pigmentation process in plants, promoting plant growth and increasing tomato tolerance to salt stress. The osmoregulatory system improved and the antioxidant enzyme activities increased to counteract reactive oxygen species-induced activities inoculated with BL-EF compared with those not inoculated with BL-EF. In addition, the inoculation with BL-EF strains increased soil enzyme activities and enhanced nutrients availability in the soil for plants uptake. In conclusion, the inoculation of ACC deaminase-producing strain BL-EF holds immense potential to alleviate salt stress in tomato plants, offering significant benefits to the agricultural sector.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109764"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Source ability is regulated by THOUSAND-GRAIN WEIGHT 6 in rice

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109760

Tatsuki Akabane , Shinichiro Kawawa , Masahiro Noguchi , Genki Horiguchi , Etsuko Katoh , Ken Ishimaru , Naoki Hirotsu

{"title":"Source ability is regulated by THOUSAND-GRAIN WEIGHT 6 in rice","authors":"Tatsuki Akabane , Shinichiro Kawawa , Masahiro Noguchi , Genki Horiguchi , Etsuko Katoh , Ken Ishimaru , Naoki Hirotsu","doi":"10.1016/j.plaphy.2025.109760","DOIUrl":"10.1016/j.plaphy.2025.109760","url":null,"abstract":"<div><div>An indole-3-acetic acid (IAA)-glucose hydrolase, THOUSAND-GRAIN WEIGHT 6 (TGW6), negatively regulates rice grain weight and starch accumulation before heading. A 1-bp deletion in <em>tgw6</em> results in loss of function and enhances grain size and yield. Thus, <em>TGW6</em> has been a target for breeding strains with increased rice yield. Although the effect of loss of <em>TGW6</em> function on sink size has been well understood, its impact on source ability (the ability to produce carbohydrates from leaves and supply to sink organs, referred to as shoot carbohydrate accumulation here) has been unclear. Here, we investigated the starch content of leaves, gene expression and carbohydrate translocation using cv. Koshihikari and a near-isogenic line carrying <em>tgw6</em> (NIL(<em>TGW6</em>)). We found that NIL(<em>TGW6</em>) accumulated more starch in lower leaf sheaths than cv. Koshihikari. Gene analysis of lower leaf sheaths from both lines indicated that the expression of starch synthesis-related genes was up-regulated, and those involved with starch degradation were down-regulated in the NIL(<em>TGW6</em>) line. Measurements of changes in carbohydrate accumulation indicated that the loss of <em>TGW6</em> function activated carbohydrate translocation and that starch accumulation in the leaf sheath contributed directly to the increase in starch uploaded to the panicles. These results provide new insights into <em>TGW6</em> function and how it affects the source ability of rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109760"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional diversity of two apple paralogs MADS5 and MADS35 in regulating flowering and parthenocarpy

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109763

Yanfang Yan , Peiyi Dang , Bingning Tian , Ying Chen , Xiaoning Li , Fengwang Ma , Jia-Long Yao , Pengmin Li

{"title":"Functional diversity of two apple paralogs MADS5 and MADS35 in regulating flowering and parthenocarpy","authors":"Yanfang Yan , Peiyi Dang , Bingning Tian , Ying Chen , Xiaoning Li , Fengwang Ma , Jia-Long Yao , Pengmin Li","doi":"10.1016/j.plaphy.2025.109763","DOIUrl":"10.1016/j.plaphy.2025.109763","url":null,"abstract":"<div><div>MADS-box genes play important roles in plant development, especially flowering and fruiting. In this study, we identified 54 type I and 69 type II MADS-box genes from the apple reference genome ‘GDDH13’. The type II MADS-box genes were further divided into 12 closely related subgroups, each exhibiting similar gene structures and conserved domains. Among these, two genes, <em>MADS35</em> and <em>MADS5,</em> belonging to APETALA1 (AP1) subfamily, were found to be predominantly expressed in apple fruit. To explore their functions, transgenic apple plants with altered expression of these genes were produced. Overexpression of <em>MADS35</em> induced early flowering, while overexpression of <em>MADS5</em> induced both early flowering and parthenocarpy. Transcriptome analysis suggested that the parthenocarpy observed in the transgenic apple plants might be associated with changes of gene expression within the auxin, GA, ABA, and ethylene signaling pathways. MADS5 and MADS35, although paralogs, differ by one amino acid in the MADS-domain and six amino acids in the K-domain, which could account for their function diversity in regulating apple fruiting. In summary, the present study provides a comprehensive analysis of MADS-box genes in apple and lays the foundation for future efforts to shorten the juvenile stage and enhance parthenocarpy-related traits in apple plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109763"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole plant transpiration responses of common bean (Phaseolus vulgaris L.) to drying soil: Water channels and transcription factors

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109759

H. Cordoba-Novoa , B. Zhang , Y. Guo , M.M. Aslam , F.B. Fritschi , V. Hoyos-Villegas

{"title":"Whole plant transpiration responses of common bean (Phaseolus vulgaris L.) to drying soil: Water channels and transcription factors","authors":"H. Cordoba-Novoa , B. Zhang , Y. Guo , M.M. Aslam , F.B. Fritschi , V. Hoyos-Villegas","doi":"10.1016/j.plaphy.2025.109759","DOIUrl":"10.1016/j.plaphy.2025.109759","url":null,"abstract":"<div><div>Common bean (<em>Phaseolus vulgaris</em> L.) is the main legume crop for direct human consumption worldwide. Among abiotic factors affecting common bean, drought is the most limiting. This study aimed at characterizing genetic variability and architecture of transpiration, stomatal regulation and whole plant water use within the Mesoamerican germplasm. A critical fraction of transpirable soil water (FTSWc) was estimated as the inflection point at which NTR starts decreasing linearly. Genome-wide association (GWA) analyses for mean NTR and FTSWc were performed. High variation on mean NTR and FTSWc was found among genotypes. Unreported genomic signals controlling the variation of these traits were identified on <em>Pv</em>01 and <em>Pv</em>07 some located in intergenic, intronic and exonic regions. A set of novel candidate genes and putative regulatory elements located in these QTL were identified. Some of the genes have been previously reported to be involved in abiotic tolerance in model species, including some of the five transcription factors (TF) identified. Four candidate genes, one with potential water transportation activity and three TFs were validated. The gene <em>Phvul.001G108800,</em> an aquaporin SIP2-1 related gene, showed water channel activity through oocyte water assays. Mutant <em>Arabidopsis thaliana</em> (<em>Ath</em>) lines for the homologous genes of common bean were evaluated in transpiration experiments. Two of the three evaluated TFs, UPBEAT1 and C2H2-type ZN finger protein, were involved in the control of transpiration responses to drying soil. Our results provide evidence of novel genes to accelerate the drought tolerance improvement in the crop and study the physiological basis of drought response in plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109759"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plastidial metabolites and retrograde signaling: A case study of MEP pathway intermediate MEcPP that orchestrates plant growth and stress responses

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-03-05 DOI: 10.1016/j.plaphy.2025.109747

Shagun Bali , Sumanta Mohapatra , Rahul Michael , Rashmi Arora , Vivek Dogra

{"title":"Plastidial metabolites and retrograde signaling: A case study of MEP pathway intermediate MEcPP that orchestrates plant growth and stress responses","authors":"Shagun Bali , Sumanta Mohapatra , Rahul Michael , Rashmi Arora , Vivek Dogra","doi":"10.1016/j.plaphy.2025.109747","DOIUrl":"10.1016/j.plaphy.2025.109747","url":null,"abstract":"<div><div>Plants are frequently exposed to environmental stresses. In a plant cell, chloroplast acts as machinery that rapidly senses changing environmental conditions and coordinates with the nucleus and other subcellular organelles by exchanging plastidial metabolites, proteins/peptides, or lipid derivatives, some of which may act as retrograde signals. These specific plastidial metabolites include carotenoid derivatives, isoprenes, phosphoadenosines, tetrapyrroles, phytohormone (like salicylic acid), and reactive electrophile species (RES), which mediate retrograde communications to sustain stress conditions. The methylerythritol phosphate (MEP) pathway is an essential and evolutionarily conserved isoprenoid biosynthetic pathway operating in bacteria and plastids, synthesizing metabolites such as terpenoids, gibberellins, abscisic acid, phytol chain of chlorophyll, carotenoids, tocopherols, and glycosides. The MEP pathway is susceptible to oxidative stress, which results in the overaccumulation of its intermediates, such as methylerythritol cyclodiphosphate (MEcPP). Recent studies revealed that under stress conditions, leading to its accumulation, MEcPP mediates retrograde signaling that alters the nuclear gene expression, leading to growth inhibition and acclimation. This review covers aspects of its generation, signaling, mechanism of action, and interplay with other factors to acquire adaptive responses during stress conditions. The review highlights the importance of plastids as sensors of stress and plastidial metabolites as retrograde signals communicating with nucleus and other sub-cellular organelles to regulate plants’ response to different stress conditions.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109747"},"PeriodicalIF":6.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0