Plant Physiology and Biochemistry最新文献_第9页

Planting resilience: One-Carbon metabolism and stress responses 植物恢复力：单碳代谢和胁迫响应

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-28 DOI: 10.1016/j.plaphy.2025.109966

Liliana E. García-Valencia , Sara M. Garza-Aguilar , Perla A. Ramos-Parra , Rocío I. Díaz de la Garza

{"title":"Planting resilience: One-Carbon metabolism and stress responses","authors":"Liliana E. García-Valencia , Sara M. Garza-Aguilar , Perla A. Ramos-Parra , Rocío I. Díaz de la Garza","doi":"10.1016/j.plaphy.2025.109966","DOIUrl":"10.1016/j.plaphy.2025.109966","url":null,"abstract":"<div><div>One-carbon (1C) metabolism is a central biochemical pathway that plays a crucial role in methylation reactions, amino acid synthesis, and nucleotide production, making it essential for plant growth. Recent advances in omics technologies, including transcriptomics, proteomics, and metabolomics, have provided comprehensive insights into the regulation of 1C metabolism in wheat, one of the world's main crops, and in the model plant Arabidopsis. Genetic manipulation through overexpression and loss-of-function studies has further revealed the roles of specific genes in modulating 1C fluxes and regulating key intermediates, such as methionine, S-adenosyl methionine, and folates. These studies have also demonstrated changes in methylation patterns as well as disruptions in growth and nutrient homeostasis. The integration of these analyses has highlighted complex feedback mechanisms within 1C metabolism that coordinate responses to environmental and developmental signals. Notably, enzymes such as serine hydroxymethyltransferase and S-adenosylmethionine synthetase have emerged as critical nodes, linking 1C metabolism with broader metabolic networks, including nitrogen and sulfur metabolism. This review synthesizes findings from recent omics and genetic studies to outline the dynamic regulation of 1C metabolism, offering a comprehensive framework for exploring its potential applications in crop improvement.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109966"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sucrose transporter systems in cotyledons (or pre-existing leaves), as integrators of multiple signals, regulate stomatal development of all leaves 子叶中的蔗糖转运系统作为多种信号的整合者，调节着所有叶片的气孔发育

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-28 DOI: 10.1016/j.plaphy.2025.109968

Zi-Meng Yao , Hu-Hui Chen , Chen Wang

{"title":"Sucrose transporter systems in cotyledons (or pre-existing leaves), as integrators of multiple signals, regulate stomatal development of all leaves","authors":"Zi-Meng Yao , Hu-Hui Chen , Chen Wang","doi":"10.1016/j.plaphy.2025.109968","DOIUrl":"10.1016/j.plaphy.2025.109968","url":null,"abstract":"<div><div>The dynamic optimization of photosynthetic production, which includes the synthesis of sucrose and glucose, is crucial for maintaining the balance between source and sink organs. This balance, in turn, determines plant growth, development, acclimation, and stress responses. The optimization of photosynthetic efficiency largely depends on the efficient transport of sugars produced through photosynthesis from the leaves. Stomata are pores found in the epidermis of stems or leaves that modulate both plant gas exchange and water/nutrient uptake. It has been investigated that the molecular mechanisms by which the stomatal development of systemic leaves is synergistically controlled by sucrose transporter systems enhance plant acclimation and stress tolerance. In this review, we summarize the current knowledge concerning the regulation of sugar signaling-mediated stomatal development and sucrose transport, focusing on the model species <em>Arabidopsis thaliana</em> and crop plants. This review provides novel insights into how sucrose transporter systems within cotyledons (or pre-existing leaves), as integrators of multiple signals, control the stomatal development of all leaves (including cotyledons or pre-existing leaves) under diverse exogenous and endogenous signals, to elevate plant acclimation and stress responses. This is achieved by integrating both exogenous and endogenous signals to modulate the process.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109968"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the interplay between plant miRNAs and plant secondary metabolites: A new frontier in cross- kingdom regulatory mechanisms 揭示植物mirna与植物次生代谢物之间的相互作用：跨王国调控机制的新前沿

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-28 DOI: 10.1016/j.plaphy.2025.109965

Yamini Agarwal , Pammi Gauba , Vibha Rani

{"title":"Unravelling the interplay between plant miRNAs and plant secondary metabolites: A new frontier in cross- kingdom regulatory mechanisms","authors":"Yamini Agarwal , Pammi Gauba , Vibha Rani","doi":"10.1016/j.plaphy.2025.109965","DOIUrl":"10.1016/j.plaphy.2025.109965","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are also known as single-stranded RNAs with 18–24 nucleotides and exhibit substantial conservation. They represent a class of innate RNAs that are essential for plant cell development, division, differentiation, proliferation, and death. The reported pharmacological effects of plant-derived secondary metabolites contribute to their therapeutic potential. Plant-derived miRNAs have drawn considerable interest as a result of their active involvement in these plant secondary metabolites (PSM). PSMs can be absorbed via diet, and exert a wide range of their therapeutic potential, via exogenous and endogenous interactions. The recent identification of plant miRNAs in controlling the expression of certain genes in mammals has attracted a lot of attention and created new opportunities for studying cross-kingdom regulatory mechanisms in biological research. This review discusses the role of miRNAs in plants, with focus on PSMs via cross-kingdom. The aim is to provide a conceptual theoretical framework based on the involvement of plant miRNA with secondary metabolites and being used as a transfer molecule for cross-kingdom gene regulation. Plant miRNAs' diverse expression patterns and ability to affect several physiological and developmental processes make them promising candidates for advancing preclinical research.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109965"},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling a century-old mystery: The role of Ophiostoma quercus in oak decline 揭开一个世纪的谜团：栎树在栎树衰亡中的作用

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-27 DOI: 10.1016/j.plaphy.2025.109948

Ivan Milenković , Dragan Karadžić , Slobodan Milanović , Vesna Golubović Ćurguz , Katarzyna Sikora , Zlatan Radulović , Vladimír Račko , František Kačík , Ján Kováč , Tomáš Toma , Martin Černý , Jaroslav Ďurkovič

{"title":"Unraveling a century-old mystery: The role of Ophiostoma quercus in oak decline","authors":"Ivan Milenković , Dragan Karadžić , Slobodan Milanović , Vesna Golubović Ćurguz , Katarzyna Sikora , Zlatan Radulović , Vladimír Račko , František Kačík , Ján Kováč , Tomáš Toma , Martin Černý , Jaroslav Ďurkovič","doi":"10.1016/j.plaphy.2025.109948","DOIUrl":"10.1016/j.plaphy.2025.109948","url":null,"abstract":"<div><div>The role of <em>Ophiostoma quercus</em> in oak decline, a significant threat to European oak ecosystems, has been debated for nearly a century. This long-term field experiment assessed the aggressiveness of <em>O. quercus</em> on <em>Quercus petraea</em> and monitored both fungal spread and tree defense responses, combining pathology, microscopy, X-ray tomography, FTIR, HPLC and proteome analyses. Fifty-nine months post-inoculation, 30 % of trees exhibited decline symptoms, while 70 % displayed extensive cankers and lesions, 28.3 times larger than those on controls. Infected trees responded by forming tyloses, blocking water transport around the inoculation site. Following infection, increased deposition of polyphenolic compounds was observed in both barrier and reaction zones. Histopathological observations and FTIR measurements revealed enhanced local deposition of suberin, lignin, lignin-related compounds, and tannins within the lumens of ray parenchyma cells, and the cell walls of both libriform fibers and vessels. Proteomic analyses suggest that host trees are employing a salicylic acid-based defense strategy. At the tissue level, these analyses indicate a shift in metabolic pathways, with downregulation of lignin biosynthesis and upregulation of flavonoid and stilbenoid biosynthesis, as evidenced by increased chalcone synthase abundance. Our groundbreaking use of submicron-computed X-ray tomography on woody tissues could pave the way for the widespread adoption of non-destructive 3D scanning technology in plant-fungal interaction research. The findings of this study demonstrated the aggressiveness of <em>O. quercus</em> towards adult <em>Q. petraea</em> and its contribution to the widespread syndrome of oak decline.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109948"},"PeriodicalIF":6.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated analyses of the transcriptome and metabolome revealed the coloring mechanism of red-pericarp wampee (Clausena lansium) 转录组学和代谢组学的综合分析揭示了红皮黄皮的着色机制。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-26 DOI: 10.1016/j.plaphy.2025.109959

Shujun Peng, Xiaoyue Zhu, Jiaxuan Chen, Jiayi Chen, Xinglong Hu, Jiale Wu, Zhike Zhang, Jietang Zhao, Guibing Hu, Irfan Ali Sabir, Yonghua Qin

{"title":"Integrated analyses of the transcriptome and metabolome revealed the coloring mechanism of red-pericarp wampee (Clausena lansium)","authors":"Shujun Peng, Xiaoyue Zhu, Jiaxuan Chen, Jiayi Chen, Xinglong Hu, Jiale Wu, Zhike Zhang, Jietang Zhao, Guibing Hu, Irfan Ali Sabir, Yonghua Qin","doi":"10.1016/j.plaphy.2025.109959","DOIUrl":"10.1016/j.plaphy.2025.109959","url":null,"abstract":"<div><div>Wampee (<em>Clausena lansium</em>), a tropical evergreen fruit from the Rutaceae family renowned for its rich nutrient profile and bioactive compounds, presents a fascinating case study in fruit coloration. However, changes in anthocyanins, and expressions of metabolism-associated genes during fruit maturation of red-pericarp wampee (‘ZR’) are not documented. In this study, metabolic and gene expression profiles of anthocyanin across different fruit developmental stages of red and yellow-pericarp wampees were analyzed. A total of 38 distinct anthocyanins were identified from the comparison of ‘ZR3’ and ‘JX3’ wampees and categorized into 17 differential anthocyanin metabolites (DAMs). Among these DAMs, fifteen were up-regulated in ‘ZR3’, while two were down-regulated compared with ‘JX3’. The delphinidin 3-[6-(4-(caffeoylrhamnosyl)glucoside)] was the predominant anthocyanins in ‘ZR’ wampee. A total of 1135 metabolics mainly including amino acid metabolites and flavonoids were detected in the ‘ZR’ and ‘JX’ wampees. Significant differences were mainly concentrated in the biosynthesis of secondary metabolites in terms of flavonoid biosynthesis, ABC transporters, and anthocyanin biosynthesis. According to the combined analyses of qRT-PCR and transcriptome, the transcript levels of <em>PAL1</em>, <em>PAL2</em>, <em>CHS1</em> and <em>UFGT1</em> in ‘ZR’ wampee were two to eight-fold higher than those in ‘JX’ wampee during fruit pigmentation. Our study offers valuable insights into the mechanisms of anthocyanin accumulation in the red pericarp of wampee which is helpful to regulate fruit coloration of wampee.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109959"},"PeriodicalIF":6.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAM5, WRKY53, and TGA5 regulate defense gene expression mediated by the volatile organic compound ethyl vinyl ketone in Arabidopsis CAM5、WRKY53和TGA5调控挥发性有机化合物乙基乙烯酮介导的拟南芥防御基因表达

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.plaphy.2025.109952

Junqing Gong , Jinchao Han , Xinyi Chen , Chunyang Jiao , Mian Zhang

{"title":"CAM5, WRKY53, and TGA5 regulate defense gene expression mediated by the volatile organic compound ethyl vinyl ketone in Arabidopsis","authors":"Junqing Gong , Jinchao Han , Xinyi Chen , Chunyang Jiao , Mian Zhang","doi":"10.1016/j.plaphy.2025.109952","DOIUrl":"10.1016/j.plaphy.2025.109952","url":null,"abstract":"<div><div>Plants produce ethyl vinyl ketone (evk) in response to biotic stress; however, the downstream defense responses triggered by evk remain unclear. Our research utilizing reverse-transcription quantitative PCR showed that evk treatment antagonized the effects of salicylic acid (SA) and jasmonic acid (JA) and that CALMODULIN 5 (CAM5) positively regulated <em>WRKY53</em> expression, which, in turn, positively regulated the expression of SA-related genes. Additionally, the GST-pulldown assay, yeast two-hybrid assays, and luciferase complementation test all suggested that CAM5 and WRKY53 physically interacted and formed a complex that was unlocked by Ca<sup>2+</sup> to release WRKY53. Electrophoretic mobility shift and dual-luciferase reporter assays demonstrated that WRKY53 enhanced the binding of TGA5 to the <em>PATHOGENESIS-RELATED 1</em> (<em>PR1</em>) promoter, thereby enhancing the expression of the defense gene <em>PR1</em>. Conversely, WRKY53 diminishes the binding of TGA5 to the PLANT DEFENSIN 1.2 (<em>PDF1.2</em>) promoter and inhibits <em>PDF1.2</em> expression. As the expression of <em>WRKY53</em> decreases in later stages, this inhibitory effect weakens, allowing TGA5 to positively activate <em>PDF1.2</em> expression. In this study, we propose a framework in which evk, as a resistance-inducing substance, facilitates the plant’s ‘rescue' through the regulation of defense-related gene expressions in Arabidopsis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109952"},"PeriodicalIF":6.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and characterization of CONSTANS-like transcription factors reveal that three CsCOLs regulate the cannabinoid biosynthesis in Cannabis constans样转录因子的全基因组鉴定和表征表明，三种CsCOLs调节大麻素的生物合成

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-24 DOI: 10.1016/j.plaphy.2025.109942

Maolun Gao , Shanshan Chen , Lingzhe Kong , Liwei Wang , Xiangxiao Meng , Ziyan Xie , Zhichao Xu , Yaolei Mi

{"title":"Genome-wide identification and characterization of CONSTANS-like transcription factors reveal that three CsCOLs regulate the cannabinoid biosynthesis in Cannabis","authors":"Maolun Gao , Shanshan Chen , Lingzhe Kong , Liwei Wang , Xiangxiao Meng , Ziyan Xie , Zhichao Xu , Yaolei Mi","doi":"10.1016/j.plaphy.2025.109942","DOIUrl":"10.1016/j.plaphy.2025.109942","url":null,"abstract":"<div><div>Cannabis (Cannabis <em>sativa</em> L.) has been cultivated as a versatile industrial crop for millennia, serving for food, fiber, and medicine. Cannabinoids are characteristic medicinal active compounds in cannabis, mainly being rich in female inflorescences. CONSTANS-like (COL) transcription factors are primarily involved in the photoperiod process of flowering plants. However, knowledge about their regulatory mechanism for secondary metabolites is limited. Eleven CsCOLs were identified from the cannabis genome based on the phylogenetic relationship and conserved domains in this study. The number of CsCOLs in cannabis showed apparent contraction and their functional divergence. <em>CsCOL1, CsCOL5,</em> and <em>CsCOL7</em> exhibited high expression in flowers and bracts and their overexpression elevated the content of CBDA and CBGA by promoting the expression of related structural genes involved in cannabinoid biosynthesis. In addition, CsCOL7 was bound to the promoters of four structural genes, including <em>CsAAE</em>, <em>CsOLS</em>, <em>CsPT4</em>, and <em>CsCBDAS</em>, to regulate their gene expression in the manner of repressing the upstream and activating the downstream genes. CsCOL1 positively regulated the expression of <em>CsPT4</em> and <em>CsCBDAS</em>. In addition, CsCOL5 positively regulated the expression of <em>CsOLS</em> and <em>CsPT4</em> via binding of their promoters. Here, we explored the potential transcription regulation mechanism of CsCOLs in cannabinoid biosynthesis in <em>C. sativa</em> for the first time, even though a more profound investigation should be conducted in cannabis plants in the future. The findings expanded the knowledge of COLs in regulating secondary metabolites and provided insights into CsCOLs in cannabinoid biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109942"},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationships between leaf morphologies and stomatal traits of Achnatherum splendens under different soil moistures in semiarid Northern China 华北半干旱地区不同土壤湿度下芨芨草叶片形态与气孔性状的关系

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-24 DOI: 10.1016/j.plaphy.2025.109947

Yuting Shen , Yuxuan Liu , Yang Gao , Yuhan Liu , Tianxiao Ma , Chunwang Xiao

{"title":"Relationships between leaf morphologies and stomatal traits of Achnatherum splendens under different soil moistures in semiarid Northern China","authors":"Yuting Shen , Yuxuan Liu , Yang Gao , Yuhan Liu , Tianxiao Ma , Chunwang Xiao","doi":"10.1016/j.plaphy.2025.109947","DOIUrl":"10.1016/j.plaphy.2025.109947","url":null,"abstract":"<div><div>The interplay between leaf and stomata is fundamental for the exchange of carbon and water between plants and the atmosphere. This relationship is pivotal for comprehending the connections between the physical structure and physiological metabolism of plant leaves under various conditions. A study examining the relationship between leaf morphological traits and stomatal traits of the <em>Achnatherum splendens</em> under varying natural soil moisture conditions was conducted along the southern shore of Hulun Lake in Inner Mongolia. In our study, stomatal density (SD) and stomatal relative area (SRA) significantly decreased, while stomatal width (SW), leaf length (LL), leaf area (LA) and leaf dry weight (LDW) increased correspondingly with rising soil moisture. A significant positive correlation between the stomatal traits and leaf morphological traits was observed through principal component analysis (PCA) and permutation test, indicating that the functional traits of leaf morphologies and stomata of <em>A</em>. <em>splendens</em> exhibit a positive relationship. The results reveal the resource utilization strategies of <em>A</em>. <em>splendens</em> in response to varying soil water conditions, offering a scientific data foundation and theoretical support for the management of <em>A</em>. <em>splendens</em> grassland, ecological restoration, and the strategic planning of animal husbandry in Inner Mongolia.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109947"},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of PcHD8 from Pogostemon cablin related to the regulation of trichome development 广藿香中与毛状体发育调控相关的PcHD8的鉴定与表征

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-23 DOI: 10.1016/j.plaphy.2025.109944

Xiaoqi Peng , Jin Huang , Yaru Lu , Yingying Liang , Lu Yang , Yuwei Zhang , Qixuan Huang , Huili Lai , Wenru Wu

{"title":"Identification and characterization of PcHD8 from Pogostemon cablin related to the regulation of trichome development","authors":"Xiaoqi Peng , Jin Huang , Yaru Lu , Yingying Liang , Lu Yang , Yuwei Zhang , Qixuan Huang , Huili Lai , Wenru Wu","doi":"10.1016/j.plaphy.2025.109944","DOIUrl":"10.1016/j.plaphy.2025.109944","url":null,"abstract":"<div><div><em>Pogostemon cablin</em>, a herbaceous plant of the <em>Lamiaceae</em> family, is widely recognized for the medicinal and industrial applications of its dried aerial parts. The plant's surface is densely populated with trichomes, which are believed to be the primary sites for the biosynthesis and accumulation of patchouli essential oil. However, the molecular mechanisms underlying the development of these trichomes in <em>P</em>. <em>cablin</em> remain largely unexplored. This study employed transcriptomic sequencing to identify and characterize genes co-regulated in trichome development and essential oil biosynthesis in <em>P. cablin</em>. Notably, we report the first identification of <em>PcHD8</em>, a member of the HD-ZIP gene family. Homologous genes, such as <em>AaHD8</em> in <em>Artemisia annua</em> and <em>SlHD8</em> in <em>Solanum lycopersicum</em>, have been shown to promote both trichome formation and secondary metabolite synthesis. We cloned <em>PcHD8</em> and overexpressed it in <em>Nicotiana tabacum</em>, which resulted in a significant increase in trichome density compared to the control. Conversely, silencing <em>PcHD8</em> via virus-induced gene silencing (VIGS) in <em>P. cablin</em> led to a marked reduction in trichome density. GC-MS analysis revealed a significant decline in the key biomarkers of patchouli essential oil, patchouli alcohol and pogostone, following <em>PcHD8</em> silencing. These findings establish <em>PcHD8</em> as a key positive regulator of trichome development and essential oil biosynthesis. This study offers critical insights into the genetic regulation of plant traits affecting medicinal quality. Our findings highlight <em>PcHD8</em> as a promising candidate gene for molecular breeding in <em>P. cablin</em> and may provide a reference for research on other trichome-rich, volatile oil-producing plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109944"},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation 百香果SWEET基因家族的鉴定和特征揭示了PeSWEET3参与可溶性糖积累

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-04-23 DOI: 10.1016/j.plaphy.2025.109943

Rui Ren , Yuxuan Chen , Xiao Yu , Xianrui Peng , Lihui Zeng , Ting Fang

{"title":"Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation","authors":"Rui Ren , Yuxuan Chen , Xiao Yu , Xianrui Peng , Lihui Zeng , Ting Fang","doi":"10.1016/j.plaphy.2025.109943","DOIUrl":"10.1016/j.plaphy.2025.109943","url":null,"abstract":"<div><div>The sugars will eventually be exported transporters (SWEET) family is a class of sugar transporters that play crucial roles in plant growth, reproduction, and stresses response. The characteristics of 21 <em>PeSWEETs</em>, identified in passion fruit (<em>Passiflora edulis</em> Sims) and divided into four clades, were evaluated. Structural feature analysis showed that exon numbers ranged from 4 to 12, while motif 1, 2, and 3 were highly conserved. Analysis of RNA-seq from different tissues revealed that <em>PeSWEETs</em> showed tissue-specificity. Additionally, <em>Cis</em>-acting regulatory element analysis indicated that abundant hormone-response and stress response elements were enriched in promoters of <em>PeSWEETs</em>. Exogenous abscisic acid (ABA) treatment led to an increase in the soluble sugar accumulation and up-regulated the expression level of <em>PeSWEET3</em>. The PeSWEET3 protein localizes on the plasma membrane and exhibits transport activity of fructose, glucose, and mannose. Furthermore, ectopic expression of <em>PeSWEET3</em> significantly enhanced soluble sugar accumulation in leaves of transgenic tobacco. Collectively, these results lay a solid foundation for further exploration on the role of <em>PeSWEETs</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109943"},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0