Plant Physiology and Biochemistry最新文献

{"title":"The essential role of the hickory StMADS11 subfamily in flower organogenesis and flowering time in Arabidopsis","authors":"Caiyun Li ,&nbsp;Zhengfu Yang ,&nbsp;Zhichao Sun,&nbsp;Di Wu,&nbsp;Bo Zhang,&nbsp;Hongmiao Jin,&nbsp;Kean-Jin Lim,&nbsp;Zhengjia Wang","doi":"10.1016/j.plaphy.2024.109402","DOIUrl":"10.1016/j.plaphy.2024.109402","url":null,"abstract":"<div><div>The StMADS11 subfamily genes play a crucial role in regulating flowering time, flower development, and bud dormancy in plants. These genes exhibit functional differences between annual and perennial woody plants. In hickory (<em>Carya cathayensis</em> Sarg.), the specific roles of these genes in flowering regulation have not been elucidated. In this study, we identified five StMADS11 subfamily genes in the hickory genome, designated as <em>CcSVP-like</em>, <em>CcAGL24-like1</em>, <em>CcAGL24-like2</em>, <em>CcJOINTLESS-like1</em>, and <em>CcJOINTLESS-like2</em>, based on their clustering characteristics. Sequence analyses revealed distinct structural features in this subfamily, including differences in intron length, C domain, and conserved motifs. Transcript analysis indicated high expression levels of these genes in female flower buds, along with a notable seasonal expression pattern. Overexpression studies on <em>Arabidopsis</em> have demonstrated that the StMADS11 subfamily genes lead to various floral organ and pod anomalies. Specifically, overexpression of <em>CcSVP-like</em> resulted in delayed flowering, while overexpression of <em>CcAGL24-like1</em>, <em>CcAGL24-like2</em>, <em>CcJOINTLESS-like1</em>, and <em>CcJOINTLESS-like2</em> promoted flowering. Protein interaction studies have shown that the StMADS11 subfamily proteins bind to the CcFUL-like protein. Notably, CcFUL-like, CcSVP-like, CcJOINTLESS-like1, and CcJOINTLESS-like2 proteins were able to bind to the <em>CcSOC1-like</em> promoter and suppress its expression. Our findings elucidate the distinct roles of the StMADS11 subfamily genes in flower development and timing, contribute to developing the current understanding of flowering regulation in hickory, and offer a foundation for further studies in perennial woody plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109402"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis and functional identification of transfer RNA-derived fragments in grape leaves exposed to UV-C radiation","authors":"Yang Li ,&nbsp;Lingchao Kong ,&nbsp;Huayuan Mu ,&nbsp;Jiayu Wang ,&nbsp;Furui Li ,&nbsp;Yangfu Kuang ,&nbsp;Wei Duan ,&nbsp;Peige Fan ,&nbsp;Ling Yuan ,&nbsp;Zhenchang Liang ,&nbsp;Lijun Wang","doi":"10.1016/j.plaphy.2024.109425","DOIUrl":"10.1016/j.plaphy.2024.109425","url":null,"abstract":"<div><div>Transfer RNA-derived fragments (tRFs) are noncoding small RNAs derived from transfer RNAs (tRNAs) in microorganisms, animals and plants. In plants, tRFs are known to respond to environmental stimuli, including heat, oxidative stress and UV radiation; however, their specific functions in horticultural plants, such as grapevine, remain poorly understood. In this study, we used RNA-seq to identify differentially expressed genes (DEGs) in grape leaves exposed to UV-C radiation. A total of 1329 and 8055 of genes were differentially expression after 1 and 6 h of UV-C treatment, respectively. We identified a large number of secondary metabolism-related genes in the DEGs, including genes involved in stilbene and flavonoid biosynthesis. Noticeably, the stilbene biosynthesis-related gene was induced earlier than the other genes in the phenylalanine metabolic pathway. We also conducted small RNA-seq and identified differentially expressed (DE) miRNAs and their targets. To explore whether the tRFs involved in UV-C response, further analysis of the small RNA-seq data revealed 23 down-regulated and 41 up-regulated DE tRFs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that the target genes of these tRFs are involved in multiple biological processing, including hormone signal transduction and metabolite synthesis. To validate the function of <em>tRFs</em>, <em>tRF39</em> and <em>tRF45</em> were selected and overexpressed in tobacco leaves, and the expression levels of their target genes were inhibited. Our study suggests that the tRFs may regulate multiple biological processes in response to UV-C exposure in grapevine. Our findings provide a foundation for further elucidating the regulatory mechanisms of tRFs in horticultural crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109425"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined analysis of transcriptomics and metabolomics showed that SNAC4 and SNAC9 are negative regulators of the resistance to Botrytis cinerea in tomato.","authors":"Yijie Sun, Zhengyu Huang, Xiaoyang Zhao, Linxiang Qiao, Zhaohui Xue, Ruoyi Gao, Bo Peng, Caie Wu, Xiaohong Kou","doi":"10.1016/j.plaphy.2024.109447","DOIUrl":"10.1016/j.plaphy.2024.109447","url":null,"abstract":"<p><p>NAC (NAM, ATAF1/2 and CUC2) is a transcription factor which contributes to the response for both biotic and abiotic stresses. In this study, the regulatory effects and potential mechanisms of SNAC4/9 on resistance to Botrytis cinerea (B. cinerea) were investigated by the differences in physiological and biochemical indices as well as transcriptional and metabolic profiles between SNAC4/9 overexpressed (OE-SNAC4/9) and wild-type (WT) tomato fruit inoculated with B. cinerea. The results showed that OE-SNAC4/9 accelerated the infection to tomato fruit by B. cinerea. Specifically, OE-SNAC4/9 mediated the differential expression of genes related to defense signaling such as ROS, phytohormones (SA and JA) and MAPK cascade, and inhibited the activities of PAL, CHI and GLU. Additionally, SNAC4/9 altered the metabolic flux redirection in each branch pathway of phenylpropane metabolism by regulating the expression of 4CL, CHS1/2, FLS and F3H, with overexpression of SNAC4/9 leading to a decrease in the accumulation of rutin, quercetin, naringenin chalcone and naringenin in tomato fruit. In conclusion, SNAC4/9 may inhibit tomato fruit resistance to B. cinerea by modulating signaling, inhibiting PRs (pathogenesis related proteins) synthesis, and altering metabolic flux flow.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109447"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of exogenous IBA on root morphology and endogenous hormone Metabolic pathways in castor seedlings under Pb/Zn stress","authors":"Siyu Chen ,&nbsp;Jiayi Liu ,&nbsp;Jinglin Zhang ,&nbsp;Lijuan Jiang ,&nbsp;Qiang Liu ,&nbsp;Xiao Zhou ,&nbsp;Peiwang Li ,&nbsp;Jingzhen Chen ,&nbsp;Yunzhu Chen ,&nbsp;Yan Yang","doi":"10.1016/j.plaphy.2024.109365","DOIUrl":"10.1016/j.plaphy.2024.109365","url":null,"abstract":"<div><div><strong><em>Ricinus communis,</em></strong> a plant of significant industrial value for its oil, is renowned for its robust root system and vigorous growth, qualities that render it an exemplary candidate for the ecological remediation of soils contaminated with heavy metals. The impact of heavy metal stress on root development is characterized by inhibition, a phenomenon whose underlying mechanisms are not fully elucidated. To shed light on this, a study was conducted wherein varying concentrations of the exogenous auxin, IBA, were applied to the roots of Ricinus communis to scrutinize its influence on the endogenous indole-3-acetic acid (IAA) metabolism in seedlings and to delineate the molecular underpinnings of its effects on root morphology. It was observed that IBA significantly amplified the total root surface area by a factor of 1.29 and increased the number of root tips by 40.11% under lead (Pb) stress, and by 32.29% and 91.19%, respectively, under zinc (Zn) stress. These findings underscore the efficacy of IBA in promoting the proliferation of lateral roots in seedlings subjected to stress induced by either Pb or Zn. Further analysis of auxin signaling pathways revealed that the presence of Pb or Zn impedes root growth and lateral root formation by perturbing auxin transporters and signaling molecules. Notably, IBA was found to foster the development of lateral roots by modulating the expression of specific transporters. Post-application of IBA, the endogenous levels of IAA in roots exhibited a 2.80-fold elevation under Pb stress, IBA stimulated the activity of key biosynthetic enzymes, such as <em>RcNIT</em> and <em>RcTAR</em>, culminating in elevated IAA levels. Conversely, under Zn stress, IBA was observed to diminish the levels of <em>RcTAR</em>, which in turn led to reduced IAA levels. These outcomes contribute to a deeper comprehension of the modulatory role of IBA in the context of heavy metal stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109365"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical, micro and ultrastructural changes in vanilla pods (Vanilla planifolia, Andrews) during the curing process","authors":"Alberto Peña-Barrientos ,&nbsp;Gloria Dávila-Ortiz ,&nbsp;Hugo Martínez-Gutiérrez ,&nbsp;María de Jesús Perea-Flores","doi":"10.1016/j.plaphy.2024.109377","DOIUrl":"10.1016/j.plaphy.2024.109377","url":null,"abstract":"<div><div>Vanilla is used in several industries, due to during the artisanal curing process, compounds responsible for a highly demanded aroma are synthesized. This process involves physical, biochemical, microbiological and structural changes, which through their study with high-resolution techniques allowed for deep introspection at the ultra-structural level, to identify cellular structures. Which, under conditions leading to the pod during the process, allow the synthesis, release, and storage of molecules aroma responsible. The integration of the referred changes will allow the strategies generation that lead to better use of the vanilla extract and its residue obtained after the extraction processes (∼95%) to which the sheath could be subjected. The proximal chemical analysis showed an increase in lipids (∼25%) at 10 SS (drying-sweating cycles) associated with oleoresins and aroma responsible compounds. Proteins increased (∼50%), a result of the catalytic activity of the enzymes present and associated with the endophytic flora. β-glucosidase associated with the synthesis of aromatic compounds, increased their activity at 10 SS (196 IU/mL). The mesocarp showed a folding and shrinkage of (∼50%), evaluated by SEM and image analysis. Using CLSM, the major compounds in the bean were located and their relationship with the micro and ultra-structure. Using TEM, plastoglobules responsible for the odorant compounds accumulation and plasmodesmata for their transport and storage were identified. The comprehensive study of the curing process and the phenomena conjunction involved, allowed the process key stage identification, important for the proposal of strategies that lead to the optimal use of the metabolites and their residues.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109377"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of drought stress on the secondary metabolism of Scutellaria baicalensis Georgi and the function of SbWRKY34 in drought resistance","authors":"Tong Zhang ,&nbsp;Caijuan Zhang ,&nbsp;Wentao Wang ,&nbsp;Suying Hu ,&nbsp;Qian Tian ,&nbsp;Yunyun Li ,&nbsp;Langjun Cui ,&nbsp;Lin Li ,&nbsp;Zhezhi Wang ,&nbsp;Xiaoyan Cao ,&nbsp;Donghao Wang","doi":"10.1016/j.plaphy.2024.109362","DOIUrl":"10.1016/j.plaphy.2024.109362","url":null,"abstract":"<div><div>The pharmacological properties of the dried root of <em>Scutellaria baicalensis</em> Georgi, a Chinese medicinal herb, include antioxidant, antibacterial, and antiviral effects. In <em>S. baicalensis</em> quality assessment, concentrations of baicalin, wogonoside, baicalein, and wogonin in the root are crucial. Drought stress commonly affects the biomass and build-up of active compounds in medicinal sections of medicinal plants and thus their quality. The molecular mechanisms underlying the response of <em>S. baicalensis</em> to drought stress remain unexplored. To delve into the impacts of drought stress on the growth and metabolic processes of <em>S. baicalensis</em>, as well as to unravel the underlying molecular mechanisms. We found prolonged and intensified drought treatment causes an initial surge in its fresh weight, plant height, and stem diameter followed by a gradual slowdown, while malondialdehyde (MDA) content rises; while the fresh weight, length, superoxide dismutase (SOD), and catalase (CAT) activities peak before declining, and the root's diameter continuously narrows. In this study, flavonoid index ingredient levels in <em>S. baicalensis</em> initially decreased, then rose as the drought duration extended, followed by a notable post-rehydration increase in baicalin, wogonoside, and baicalein content and decrease in levels of wogonin and oroxylin A. Transcriptome sequencing and KEGG analysis revealed a significant enrichment of DEGs involved in phenylpropanoid biosynthesis and plant hormone signal transduction pathways. The expression levels of <em>SbPAL</em>, <em>SbCCL</em>, <em>Sb4CL</em>, <em>SbCHI</em>, <em>SbFNSII</em>, <em>SbF6H,</em> and <em>SbUGT</em> genes in the flavonoid biosynthetic pathway and <em>PYR</em>/<em>PYL</em>, <em>PP2C</em>, <em>ABF</em>, and <em>SnRK2</em> genes in the abscisic acid signal transduction pathway were significantly changed. Drought responsive <em>SbWRKY34</em> was selected for the subsequent investigation. <em>SbWRKY34</em> showed the highest level in stems, and the encoding protein was localized in the nucleus. Overexpression of <em>SbWRKY34</em> in <em>Arabidopsis thaliana</em> (OE-<em>SbWRKY34</em> lines) resulted in increased sensitivity to drought stress, with considerably reduced MDA content and elevated SOD and CAT activities. Concurrently, the expression levels of <em>AtCAT3</em>, <em>AtDREB</em>, <em>AtRD22</em>, <em>AtRD29A</em>, and <em>AtRD29B</em> were significantly reduced in these lines, suggesting that <em>SbWRKY34</em> functions to negatively regulate drought resistance in <em>A. thaliana</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109362"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-light and nutrient interactions drive carbohydrate and proton pump dynamics in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze","authors":"Khairul Azree Rosli ,&nbsp;Azizah Misran ,&nbsp;Latifah Saiful Yazan ,&nbsp;Puteri Edaroyati Megat Wahab","doi":"10.1016/j.plaphy.2024.109374","DOIUrl":"10.1016/j.plaphy.2024.109374","url":null,"abstract":"<div><div><em>Agastache rugosa</em>, a perennial herb native to temperate and subtropical regions, shows remarkable adaptive strategies when exposed to varying light and nutrient conditions in tropical environments. Our study reveals new insights into the crosstalk mechanisms involving carbohydrate homeostasis, biomass allocation, and nutrient acquisition in <em>A. rugosa</em> under different environmental conditions. Treatments were two light levels; HL (high-light, 0% shade) and LL (low-light, 50% shade), and four nutrient rates; NPK1 (40 mg kg⁻¹), NPK2 (80 mg kg⁻¹), NPK3 (120 mg kg⁻¹) and NPK4 (160 mg kg⁻¹). High-light coupled with increasing nutrient levels (HL-NPK3 and HL-NPK4) promoted biomass production via increased carbon assimilation, associated with higher soluble sugar levels and higher phosphorus and potassium uptake mediated by the upregulation of plasma membrane H⁺-ATPase. Maximum carbohydrate accumulation occurred at high-light and the lowest nutrient levels (HL-NPK1), coinciding with increased nitrogen uptake and the drastically high leaf nitrogen concentration. This response was preceded by the upregulation of acid phosphatase and sucrose phosphate synthase, suggesting a compensatory mechanism to maintain nutrient and carbohydrate reserves for critical metabolic processes. Starch increase was more apparent under low-light and higher nutrient levels (LL-NPK3 and LL-NPK4), relative to invertase downregulation, indicating a shift towards carbohydrate storage rather than utilization. These findings underscore the complex interplay between sugar signaling, nutrient sensing, enzymatic actions, and proton pump activity in modulating plant adaptation to varying environmental conditions. This study also highlights the importance of understanding how non-model medicinal species like <em>A. rugosa</em> reprogram their metabolism and resource allocation in response to environmental changes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109374"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated comparative physiological and transcriptomic analyses of Elymus sibiricus L. reveal the similarities and differences in the molecular mechanisms in response to drought and cold stress.","authors":"Xinrui Li, Lili Chen, Daxu Li, Minghong You, Yingzhu Li, Lijun Yan, Jiajun Yan, Wenlong Gou, Dan Chang, Xiao Ma, Shiqie Bai, Yan Peng","doi":"10.1016/j.plaphy.2024.109459","DOIUrl":"10.1016/j.plaphy.2024.109459","url":null,"abstract":"<p><p>Drought and cold crucially affect plant growth and distribution. Plants have evolved complex molecular mechanisms to adapt to such adverse environmental conditions. This study examines two Elymus sibiricus (Es) germplasms differing in resilience to these stresses. Analyzing physiological responses and gene expression changes under drought and cold, it reveals the similarities and differences in their molecular mechanisms that underlie these responses. The results indicate that both drought stress and cold stress severely damage the integrity of the cell membrane in Es. Notably, under cold stress, the accumulation of osmotic regulation substances in Es is more significant, which may be related to the regulation of carbohydrate metabolism (CM)-related genes in cold environments. Furthermore, the response to oxidative stress triggered by cold stress in Es is partially inhibited. The enrichment analysis showed that the DEGs responsive to drought stress in Es were mainly related to the pathway of photosynthesis, whereas the DEGs responsive to cold stress were more associated with the protein processing in endoplasmic reticulum (PPER), highlighting distinct molecular responses. In addition, we discovered that the abscisic acid (ABA) signaling transduction plays a dominant role in mediating the drought resistance mechanism of Es. We have identified 86 key candidate genes related to photosynthesis, Phst, CM, and PPER, including 5 genes that can respond to both drought and cold stress. This study provides a foundation for the molecular mechanisms underlying cold and drought resistance in Es, with insight into its future genetic improvement for stress resistance.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109459"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytic acid promotes the oxidative stress tolerance of Meyerozyma (Pichia) caribbica enhancing its efficacy against natural decay and retaining the quality of table grapes.","authors":"Joice Meshi Foku, Esa Abiso Godana, Qiya Yang, Hongyin Zhang","doi":"10.1016/j.plaphy.2024.109463","DOIUrl":"10.1016/j.plaphy.2024.109463","url":null,"abstract":"<p><p>The sporogenous yeast Pichia caribbica, a part of the Meyerozyma guilliermondii species complex is found in various environmental niches due to its diverse physiological and metabolic capacities that enhance adaptation and survival. This study examined the application of phytic acid (PA) to improve the biological control efficiency of P. caribbica against natural decay and to preserve the quality of table grape berries. The mechanisms by which PA enhances P. caribbica's biocontrol efficiency were investigated. The yeast cultured in nutrient yeast dextrose broth (NYDB) with (YE) or without (Y) PA was assessed for its ability to produce biofilms and to withstand induced oxidative stress. It was observed that supplementation with 10 μmol/mL PA markedly increased the proliferation and the capacity of P. caribbica to form biofilms (OD₅₉₀ = 0.8) in vitro compared to the non-supplemented yeast (OD₅₉₀ = 0.69). Additionally, P. caribbica cultured with PA (10 μmol/mL) effectively improved tolerance to induced oxidative stress. Phytic acid pretreatment also boosted the activities of defense antioxidant enzymes including β-1,3-glucanase (GLU), catalase (CAT), and superoxide dismutase (SOD) in P. caribbica. The impact of 10 μmol/mL PA on the biocontrol efficacy of P. caribbica led to favourable changes in the physicochemical parameters of table grapes with significantly improved control of the natural decay for fruits stored at ambient and cold conditions. These findings suggest that PA enhances the biocontrol effectiveness of P. caribbica, paving the way for the development of sustainable alternative solutions to combat postharvest fungal phytopathogens. Moreover, consumers preferred table grape bunches treated with YE appreciating the overall cluster appearance, the berry's textural crunchiness, a pronounced berry fragrance, and limited decay. They prioritized fruits stored at 4 °C.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109463"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From intercropping to monocropping: The effects of Pseudomonas strain to facilitate nutrient efficiency in peanut and soil","authors":"Tianqi Wang ,&nbsp;Kunguang Wang ,&nbsp;Nanqi Wang ,&nbsp;Dongming Cui ,&nbsp;Shiqin Li ,&nbsp;Qiaofang Lu ,&nbsp;Yuanmei Zuo","doi":"10.1016/j.plaphy.2024.109378","DOIUrl":"10.1016/j.plaphy.2024.109378","url":null,"abstract":"<div><div>As an oilseed crop, the yield and quality of peanuts are severely constrained by nutrient deficiencies, particularly in calcareous soils in northern China. Maize-peanut intercropping is an effective strategy to enhance mineral nutrient efficiency in peanuts via plant-microbe interaction, but the underlying mechanisms remain elusive. Here, we conducted experiments using a Pseudomonas strain (Pse.IP6) with diverse beneficial characteristics, which was isolated from the rhizosphere of intercropped peanuts. Additionally, Pse.IP6 exhibits high phylogenetic similarity with the Amplicon Sequence Variants 48 (ASV48) which belongs to <em>Pseudomonas</em> and is positively correlated with Fe in plants and soil in intercropping. To confirm the plant growth-promoting potential of Pse.IP6 and its role in intercropping advantage, we constructed pot experiments. Results revealed that Pse.IP6 promoted shoot growth and root development, as well significantly enhanced SPAD value, net photosynthetic rate, stomatal conductance, and transpiration rate of peanut leaves. Moreover, the application of Pse.IP6 resulted in a notable accumulation of nitrogen (N), phosphorus (P), and potassium (K) in shoot and active iron (Fe) in leaves, accompanied by an increased K-N ratio. The primary reason for the nutrient promotion is the enhancement of the bioavailability of nitrate, ammonium, P, K, and Fe in the rhizosphere. Collectively, our findings demonstrate that Pse.IP6, enriched in intercropping peanut, is a plant growth-promoting bacteria, represented by transferring the intercropping advantage on nutrients activation to monocropping peanuts. Our results offer insights into plant-rhizobacteria interaction mechanisms and therefore provide a rhizobacteria-based pathway to improve nutrient efficiency and productivity of crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"Article 109378"},"PeriodicalIF":6.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}