Plant Physiology and Biochemistry最新文献

{"title":"Integrated transcriptomic and metabolomic analyses revealed that AmASMT positively regulates drought tolerance in Agropyron mongolicum by modulating melatonin biosynthesis","authors":"Jing Wang ,&nbsp;Jinqing Zhang ,&nbsp;Shuxia Li ,&nbsp;Shoujiang Sun ,&nbsp;Wenxue Song ,&nbsp;Xing Wang ,&nbsp;Xiaocong Li ,&nbsp;Juhui Yan ,&nbsp;Xueqin Gao ,&nbsp;Bingzhe Fu","doi":"10.1016/j.plaphy.2025.110528","DOIUrl":"10.1016/j.plaphy.2025.110528","url":null,"abstract":"<div><div>Global climate change has exacerbated drought stress episodes, which are emerging as a serious threat to plant growth and productivity worldwide. In this context, melatonin has emerged as a potential signaling molecule for improved drought tolerance in plants, primarily through enhanced antioxidant defenses. Here, physiological, transcriptome, and metabolome analyses were used to investigate the physiological and molecular mechanisms of melatonin in drought stress mitigation in <em>A. mongolicum</em> with both drought-tolerant and drought-sensitive genotypes. Physiological results suggest that melatonin improves drought tolerance in <em>A. mongolicum</em> primarily by enhancing the antioxidant enzyme system. Integrated transcriptomic and metabolomic analyses have demonstrated that the tryptophan metabolic pathway plays a crucial role in melatonin-mediated enhancement of drought resistance. Notably, we report on the drought-related gene <em>AmASMT</em>, which encodes a melatonin biosynthesis enzyme and contributes to drought stress tolerance in <em>A. mongolicum</em>. We found that the <em>AmASMT</em> overexpressing rice lines exhibited higher endogenous melatonin levels and increased tolerance to drought stress by promoting antioxidant systems. Our findings indicate that the <em>AmASMT</em> plays a crucial role in regulating melatonin biosynthesis <em>A. mongolicum</em> while facilitating protection against drought stress. These results shed light on the regulatory mechanism of melatonin biosynthesis related to the drought stress response <em>A. mongolicum</em>, and provides a basis for exploiting melatonin-mediated mechanisms and genetic engineering approaches to enhance plant drought tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110528"},"PeriodicalIF":5.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomic, metabolomic, and physiological analyses reveal the role of strigolactones in enhancing drought resistance in Juglans sigillata","authors":"Qing Shang ,&nbsp;Zhaoyang Shi ,&nbsp;Zhangjun Wei ,&nbsp;Shiwei Li ,&nbsp;Lei Fang ,&nbsp;Dong Huang","doi":"10.1016/j.plaphy.2025.110529","DOIUrl":"10.1016/j.plaphy.2025.110529","url":null,"abstract":"<div><div><em>Juglans sigillata</em>, an economically valuable tree species mainly cultivated in southwest China, is frequently affected by seasonal drought, which significantly impacts its growth and yield. This study investigated the role of strigolactones (SLs) in mitigating drought stress through physiological, transcriptomic, and metabolomic analyses, along with overexpression of SLs synthesis-related gene <em>JsCCD7</em> in <em>Arabidopsis thaliana</em>. Exogenous SLs spraying effectively alleviated drought adverse effects: SPAD value and relative water contents of walnut leaves increased by 47.3 % and 13.9 %, respectively; net photosynthetic rate, stomatal conductance, and transpiration rate rose by 67.5 %, 51.2 %, and 61 %, respectively; whereas malondialdehyde, hydrogen peroxide, and superoxide anion contents decreased by 9.8 %, 17 %, and 11 %, respectively. High-throughput sequencing revealed that SLs enhanced drought resistance by upregulating lignin synthesis genes and their precursors (caffeic acid and Coniferin), as well as elevating rutin and quercetin levels in the flavonoid pathway to alleviate oxidative damage and stress response. Additionally, three Arabidopsis lines overexpressing <em>JsCCD7</em> exhibited higher SLs content, total flavonoid levels, and biomass compared to wild-type under drought conditions, along with reduced reactive oxygen species damage. In summary, SLs enhanced drought resistance and tolerance, alleviated stress, and promoted growth in <em>J. sigillata</em> seedlings. This study provides a theoretical basis for improving drought resistance in <em>J. sigillata</em> through SLs-related pathways.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110529"},"PeriodicalIF":5.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analysis reveals the mechanism of cross-adaptation in Krascheninnikovia arborescens to combined drought and cold stress","authors":"Kai Cheng,&nbsp;Jie Zhang,&nbsp;WeiWei,&nbsp;Lingling Zheng,&nbsp;Yingchun Wang","doi":"10.1016/j.plaphy.2025.110481","DOIUrl":"10.1016/j.plaphy.2025.110481","url":null,"abstract":"<div><div>Cold and drought stress are important environmental factors limiting agricultural production, especially in the northwestern region of China. However, few studies have examined the responses of plants to combined drought and cold stress. Here, we analyzed the cross-adaptation of <em>Krascheninnikovia arborescens</em> to these two stresses based on physiological, comparative transcriptomics, and metabolomics analyses. Drought pretreatment significantly enhanced cold tolerance of the plants. Physiological analyses showed that combined drought and cold stress caused less cellular damage than cold stress alone, as evidenced by lower relative electrical conductivity and reduced ROS accumulation. Plants subjected to combined stress also showed improved photosynthetic parameters during the recovery stage. Transcriptomics analyses identified a large number of differentially expressed genes (DEGs) under different stress conditions. A portion of the DEGs under conditions of combined stress were common to both drought and cold stress, and these genes were involved in processes such as defense and photosynthesis. Metabolomics analysis identified metabolites, and more metabolites from the metabolic pathways of flavonoids, lignin, and carbohydrates were accumulated under combined stress conditions. Integrated transcriptomics and metabolomics analyses revealed significant enrichment of the flavonoid and phenylpropanoid biosynthesis pathways. These findings suggest that drought pretreatment enables <em>K. arborescens</em> to withstand cold stress more effectively by enhancing its antioxidant capacity, accumulating osmoprotectants, and activating specific metabolic pathways. These findings reveal novel molecular and metabolic mechanisms underlying cross-adaptation, providing potential targets for breeding multi-stress–resilient crops suited to harsh environments.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110481"},"PeriodicalIF":5.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen alleviates salt stress in maize: Genotypic variations in ion balance, antioxidant response and nitrogen metabolism","authors":"Shujie Zhang ,&nbsp;Peiyu Zhao ,&nbsp;Guangshun Zhou ,&nbsp;Pengjuan Cui ,&nbsp;Fang Li ,&nbsp;Yanlai Han ,&nbsp;Yi Wang","doi":"10.1016/j.plaphy.2025.110516","DOIUrl":"10.1016/j.plaphy.2025.110516","url":null,"abstract":"<div><div>Soil salinization severely restricts maize growth and productivity. Nitrogen application mitigates salt stress, but the underlying physiological mechanisms and genotypic variations remain unclear. This study investigated how varying N levels (0.1, 2, 10 mM) alleviate salt stress (150 mM NaCl) in two maize inbred lines with contrasting salt tolerance: Mo17 (tolerant) and B73 (sensitive). Salt stress significantly reduced plant biomass, K⁺/Na⁺ ratio, and nitrogen accumulation in both genotypes. However, increasing nitrogen supply enhanced salt tolerance by optimizing root K⁺/Na⁺ homeostasis, enhancing nitrogen metabolism enzyme activities, and improving antioxidant defenses. It is worth noting that genotypic differences dictated nitrogen-dependent response: Mo17 achieved a higher biomass at low nitrogen (2 mM) level, whereas B73 required high nitrogen (10 mM) level to achieve comparable biomass. Nitrogen application also differentially regulated antioxidant enzymes enhancing leaf peroxidase (POD) and catalase (CAT) activities under salt stress, with Mo17 showing stronger low-nitrogen adaptability. Meanwhile, nitrogen supply also promotes the activity of nitrogen metabolism enzymes and nitrogen accumulation. Correlation analysis indicated that root K⁺ contents and leaf CAT activity as key biomarkers positively linked to plant biomass. Our results demonstrate that nitrogen alleviates salt stress in maize by coordinately regulating ion balance, nitrogen metabolism, and antioxidant defense, but efficacy is genotype-dependent. These findings provide physiological insights for optimizing nitrogen management in saline soils and increasing maize yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110516"},"PeriodicalIF":5.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous salicylic acid induces endogenous SA biosynthesis, enhances osmolyte accumulation, antioxidant defense, and free radical scavenging to alleviate chilling injury in red bell pepper fruit","authors":"Amal Kadhim Hrebid ,&nbsp;Ali Asghar Hatamnia ,&nbsp;Meisam Mohammadi ,&nbsp;Mohammad Ebrahim Ranjbar","doi":"10.1016/j.plaphy.2025.110524","DOIUrl":"10.1016/j.plaphy.2025.110524","url":null,"abstract":"<div><div>Chilling injury (CI) is a major postharvest limitation for tropical and subtropical crops such as bell pepper (<em>Capsicum annuum</em> L.), reducing marketability and shelf life under low-temperature storage. This study assessed the effects of preharvest foliar application of salicylic acid (SA) at concentrations of 0, 1, 5, 10, and 15 mM on reducing CI and maintaining the postharvest quality of red bell pepper fruit (cv. ‘Westland, 2090’) stored at 4 °C. Results showed that CI symptoms progressively worsened during storage in untreated fruit, whereas SA treatments, at 5 mM, reduced CI index incidence and preserved superior visual and physicochemical quality. The 5 mM SA treatment lowered the CI index by 76 % (The CI index decreased from 4.60 to 3.06) after 24 d and extended shelf life by 9 d compared to controls. This concentration also reduced weight loss, electrolyte leakage, and the accumulation of reactive oxygen species (hydrogen peroxide and superoxide radicals) and malondialdehyde, indicating improved membrane integrity and reduced oxidative damage. Additionally, 5 mM SA-treated fruit maintained higher firmness, titratable acidity, total soluble solids, vitamin C, antioxidant capacity, and total phenolic content. These benefits correlated with enhanced activities of key antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase, and suppression of lipid-degrading enzymes such as lipoxygenase, phospholipase D, and polyphenol oxidase. Notably, SA treatment stimulated an increase in phenylalanine ammonia-lyase activity and endogenous SA levels, amplifying defense signaling pathways. Increased accumulation of osmoprotectants γ-aminobutyric acid and proline further supported improved cold stress tolerance. Together, these findings demonstrate that preharvest application of 5 mM SA is a practical and effective strategy to mitigate CI and preserve postharvest quality in red bell pepper during cold storage.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110524"},"PeriodicalIF":5.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling ZnO-NPs priming–driven salt tolerance in germinating castor seeds via integrated lipidomic and transcriptomic analyses","authors":"Peilin Han ,&nbsp;Lijuan Dai ,&nbsp;Bing Gao ,&nbsp;Jinji Han ,&nbsp;Yingxin Han ,&nbsp;Yixuan Wang ,&nbsp;Qiuying Pang ,&nbsp;Jixiang Lin ,&nbsp;Jinghong Wang","doi":"10.1016/j.plaphy.2025.110525","DOIUrl":"10.1016/j.plaphy.2025.110525","url":null,"abstract":"<div><div>Castor (<em>Ricinus communis</em>) is a globally important oilseed crop whose germination is highly sensitive to soil salinity. We assessed whether 24 h priming with 1000 mg L⁻¹ ZnO-NPs (Z2) enhances salt tolerance compared to unprimed controls (Z1) under 0–150 mM NaCl (S1–S4). Under 100 mM NaCl (S3), Z2 seeds displayed a cumulative germination percentage nearly 1.5-fold higher than the control and a 26.1 % reduction in malondialdehyde (MDA) content. Lipidomic profiling revealed ZnO-NPs priming stabilized membrane fluidity by elevating phosphatidylcholine (PC), phosphatidic acid (PA), phosphatidyl ethanolamine (PE), phosphatidylglycerol (PG), and lysophosphatidylcholine (LPC) levels and increasing overall lipid unsaturation. Transcriptomic analysis identified 349 differentially expressed genes between (Z2, S3) and (Z1, S3), and integrated pathway analysis demonstrated upregulation of “glyoxylate and dicarboxylate” and “starch and sucrose” metabolism to drive lipid-to-carbohydrate conversion. Key lipase, nonspecific lipid transfer proteins (nsLTPs), and oleosin genes were also induced, and MAPK signaling was enhanced, to sustain germination vigor under salt stress. These dual mechanisms—antioxidant-mediated membrane protection and transcriptome-guided regulation of metabolic pathways—underscore the potential of ZnO-NPs priming to enhance castor seed resilience under salt stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110525"},"PeriodicalIF":5.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of physiological-biochemical index regulation in Chardonnay grape leaf phototaxis and gene hub module mining","authors":"Fanwei Zeng,&nbsp;YanMei Li,&nbsp;Wenfang Li,&nbsp;Zonghuan Ma,&nbsp;Juan Mao,&nbsp;Baihong Chen","doi":"10.1016/j.plaphy.2025.110458","DOIUrl":"10.1016/j.plaphy.2025.110458","url":null,"abstract":"<div><div>Phototropic movement is an evolutionary strategy that increases light capture and photosynthetic efficiency in plants. Despite its importance, the physiological, biochemical, and molecular basis of pulvinus-driven leaf movement in grape species remains poorly understood. To simulate adverse light conditions, Chardonnay grapevine branches were artificially inverted, altering the natural growth orientation of the leaves. In this study, the dynamic characteristics of pulvinus-mediated leaf movement were meticulously documented, and key physiological parameters (gas exchange and water potential) were evaluated. Additionally, the daily fluctuations in H⁺-ATPase activity, electrical conductivity, ions (K⁺, Ca²⁺, Mg²⁺, Cu²⁺, Fe^2+/3+, Zn²⁺, and Mn²⁺), and hormones (IAA, GA₃, ABA, and ZT) in pulvinus were systematically measured. Transcriptomic analysis was employed to identify potential molecular hub regulatory modules involved in the photosynthetic and hormonal pathways. A significant increase in leaf gas exchange and water potential resulted from a change in leaf orientation. During pulvinus movement, H⁺-ATPase activity and extensor conductivity increased significantly, and Fe^2+/3+ exhibited antagonistic and synergistic changes with K⁺ and Mn²⁺, respectively. The IAA content in the flexor decreased gradually, whereas the GA₃ content in the extensor first decreased and then increased. A total of seven hub modules were identified among the differentially expressed genes associated with the photosynthetic and hormonal pathways. Overall, grapevines adjust leaf orientation through strategies that increase leaf gas exchange and water potential and alter the differential distribution of hormones and ions within the pulvinus. This study offers valuable insights into leaf movement's physiological and biochemical mechanisms and provides genetic resources for molecular-level research.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110458"},"PeriodicalIF":5.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous allantoin improves waterlogging tolerance of summer maize by regulating the endogenous allantoin and nitrogen metabolism","authors":"Yu-Long Zhou ,&nbsp;Ya-Wei Wang ,&nbsp;Ling-Ling Cui ,&nbsp;Dou-Dou Jin ,&nbsp;Jin-Liang Zhou ,&nbsp;Yuan Fang ,&nbsp;Yong-Ling Ruan ,&nbsp;Li-Xin Tian","doi":"10.1016/j.plaphy.2025.110507","DOIUrl":"10.1016/j.plaphy.2025.110507","url":null,"abstract":"<div><div>Waterlogging has emerged as one of the main disasters limiting maize production in the Huang-Huai-Hai region. The physiological mechanisms by which allantoin exogenously regulated waterlogging tolerance of maize seedlings remained unclear. Based on the concentration screening test, the optimal allantoin concentration was found to be 5 mM. Then, the endogenous allantoin metabolism, nitrogen metabolism, and transcriptomic profiles of three plant organs were explored. Results showed that 5 mM allantoin significantly boosted peroxidase and superoxide dismutase activities, enhanced reactive oxygen species scavenging, while increasing soluble protein and sugar accumulation across leaves, seminal roots, and adventitious roots under waterlogging stress. Compared with the control treatment, waterlogging (WL) significantly suppressed nitrate metabolism by decreasing the activities of nitrate reductase (NR), glutamate synthase, and glutamine synthetase, while downregulating NR gene expression in all organs and reducing total nitrogen content on the 6th day of waterlogging. The waterlogging plus 5 mM allantoin treatment (WL + ALN5) restored NR activity, increasing it by 46.71 %, 47.91 %, and 34.61 % in the leaves, seminal roots, and adventitious roots, respectively. Furthermore, WL + ALN5 treatment reduced the activities of xanthine dehydrogenase and uric acid oxidase in seminal and adventitious roots, while it stimulated allantoinase activity and enhanced endogenous allantoin and allantoic acid levels. The gene expression of allantoinase was downregulated in leaves but upregulated in seminal roots. This finding provides a theoretical basis for the cultivation of maize with stable waterlogging resistance and yield in the Huang-Huai-Hai region.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110507"},"PeriodicalIF":5.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas syringae infection causes metabolic changes in tomato leaves","authors":"Chunyan Chen,&nbsp;Jiahua Ye,&nbsp;Keke Zhao,&nbsp;Mingyang Hao,&nbsp;Fangfang Ma,&nbsp;Zhilong Bao","doi":"10.1016/j.plaphy.2025.110521","DOIUrl":"10.1016/j.plaphy.2025.110521","url":null,"abstract":"<div><div>Pathogen infection requires the reprogramming of host metabolism to assist the pathogen growth and stimulate host defense responses. Repression of photosynthesis is a common phenomenon during the pathogen infection, while the changes of primary metabolic pathways and carbohydrates are seldomly studied. This study aims to investigate the temporal changes of physiological parameters and primary metabolites in tomato leaves infected with <em>Pseudomonas syringae</em> pv<em>. Tomato</em> DC3000 (<em>Pst</em> DC3000). Pathogen infection triggered the reduction of pigments and starch accumulation. The production of reactive oxygen species and malondialdehyde are enhanced, and the acitvities of antioxidative enzymes are reduced after the pathogen infection. Pathogen induces the leaf cell enlargement and enhanced endoreduplication. Targeted metabolomics using GC-MS and LC-MS/MS reveals that metabolites in photosynthesis, sugar biosynthesis and TCA cycle are significantly increased between 24 and 36-h post inoculation (hpi). Overall, metabolites in photosynthesis, sugar biosynthesis and TCA cycle are more responsive to the pathogen infection than other metabolic pathways. Moreover, RNA-seq data in <em>Pst</em> DC3000-resistant and susceptible plants reveal that majority genes involved in Calvin cycle, photorespiration, sucrose and starch biosynthesis and TCA cycle have opposite transcriptional patterns after the pathogen infection. Our study illustrates drastic fluctuations of host primary metabolism during the bacterial pathogen infection, suggesting that metabolic engineering on photosynthesis, sugar biosynthesis and TCA cycle may enhance plant disease resistance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110521"},"PeriodicalIF":5.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BrRAV8 negatively modulates thermotolerance through suppressing cellulose biosynthesis in flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)","authors":"Dalian Lu,&nbsp;Lingqi Yue,&nbsp;Jiajing Zeng,&nbsp;Dengjin Kang,&nbsp;Kunhua Peng,&nbsp;Juan Li,&nbsp;Taoyu Pan,&nbsp;Min Zhong,&nbsp;Yunyan Kang,&nbsp;Xian Yang","doi":"10.1016/j.plaphy.2025.110506","DOIUrl":"10.1016/j.plaphy.2025.110506","url":null,"abstract":"<div><div>High temperature stress severely impairs the growth and development of flowering Chinese cabbage. RAV transcription factors are well-characterized regulators of plant responses to salt and drought stresses, and cellulose plays a fundamental role in stress adaptation. However, the molecular mechanism by which RAVs regulate thermotolerance through mediating cellulose biosynthesis in flowering Chinese cabbage remains to be elucidated. Herein, we systematically investigated 14 RAV transcription factors and 36 cellulose biosynthesis-related genes in flowering Chinese cabbage seedlings (21 days after sowing) under high temperature stress (42 °C). Notably, <em>BrRAV8</em>, <em>BrCESA7</em>, and <em>BrCSLB3.2</em> exhibited significant induction under heat stress. Transcriptional activation assays demonstrated that BrRAV8 lacked transcriptional activation capacity and likely functioned as a transcriptional repressor. <em>BrRAV8</em> overexpression significantly downregulated <em>BrCESA7</em> and <em>BrCSLB3.2</em> expression, reducing cellulose content and increasing reactive oxygen species (ROS) accumulation, thereby leading to decreased thermotolerance. Conversely, <em>BrRAV8</em> silencing produced the opposite effects. Additionally, silencing <em>BrCESA7</em> or <em>BrCSLB3.2</em> markedly compromised thermotolerance, accompanied by elevated ROS level and diminished cellulose accumulation. Through yeast one-hybrid, dual luciferase, and electrophoretic mobility shift assays, we confirmed that BrRAV8 directly bound to the promoters of <em>BrCESA7</em> and <em>BrCSLB3.2</em> to transcriptionally repress their expression. Collectively, our study uncovered a previously unrecognized regulatory module in which BrRAV8 inhibited the cellulose biosynthesis by suppressing the upregulation of <em>BrCESA7</em> and <em>BrCSLB3.2</em> to attenuate heat tolerance. This work not only significantly expands our understanding of the molecular regulatory network underlying heat stress response, but also identifies critical genetic resources for developing heat-resistant cultivars, ultimately contributing to improved heat tolerance and productivity in heat-stress cultivation of flowering Chinese cabbage.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110506"},"PeriodicalIF":5.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}